calcium cacl2 chlorides  (Millipore)


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    Structured Review

    Millipore calcium cacl2 chlorides
    Calcium Cacl2 Chlorides, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/calcium cacl2 chlorides/product/Millipore
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    calcium cacl2 chlorides - by Bioz Stars, 2020-02
    95/100 stars

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    Related Articles

    Centrifugation:

    Article Title: Separately or Combined, LukG/LukH Is Functionally Unique Compared to Other Staphylococcal Bicomponent Leukotoxins
    Article Snippet: Resuspended cells in 0.9% NaCl were layered over 10 mL Ficoll-Paque (GE Healthcare) and then spun at 1400 rpm at 20°C for 40 min. PMNs were isolated by centrifugation at 1000 rpm at 5°C for 6 min after lysing RBCs by 0.2% NaCl and 1.6% NaCl. .. To examine the role of calcium channels in calcium ion entry, in a set of experiments, methoxyverapamil (Sigma) was added at 100 µM or 400 µM 60 min prior to addition of BCL components.

    Positive Control:

    Article Title: CGA-N12, a peptide derived from chromogranin A, promotes apoptosis of Candida tropicalis by attenuating mitochondrial functions
    Article Snippet: Detection of calcium ion uptake To elucidate Ca2+ uptake under the effect of CGA-N12, Ca2+ probes Fura-2-AM and Rhod-2-AM (Sigma–Aldrich, Shanghai, China) were used to detect changes in the Ca2+ content of the cytosol and mitochondria, respectively [ ]. .. C. tropicalis cells (1 × 106 ) were incubated with 75 µM CGA-N12 for 0, 5, 10 and 15 h at 28°C; 10 mM H2 O2 was used as a positive control.

    Cytometry:

    Article Title: RORγt inhibition selectively targets IL-17 producing iNKT and γδ-T cells enriched in Spondyloarthritis patients
    Article Snippet: Paragraph title: Flow cytometry ... For cytokine stainings, cells were collected and restimulated for 4 h with PMA (25 ng/mL), calcium-ionomycin (1 µg/mL; both from Sigma) and brefeldin A (10 ng/mL; BD).

    Article Title: Amniotic mesenchymal cells from pre‐eclamptic placentae maintain immunomodulatory features as healthy controls
    Article Snippet: .. Therefore, cells were stimulated for 5 hrs at 37°C with phorbol‐12‐myristate‐13‐acetate (50 ng/ml), calcium‐ionomycin (CaI, 1 μg/ml) and Brefeldin A (10 μg/ml) (all from Sigma‐Aldrich), and viable cells were analysed on a flow cytometer. ..

    Article Title: Altered thymic differentiation and modulation of arthritis by invariant NKT cells expressing mutant ZAP70
    Article Snippet: Samples were acquired on a FACS Canto II flow cytometer (BD) and analyzed by means of FlowJo 10.3 software (Treestar). .. For evaluation of IFN-γ production by iNKT cells directly ex vivo, freshly isolated PBMC and SFMC were incubated for 4 h with phorbol 12-myristate 13-acetate (PMA, 25 ng/ml), calcium-ionomycin (CaI, 1 µg/ml; both from Sigma) and brefeldin A (BFA, 10 ng/ml; BD) or BFA alone (negative control).

    Blocking Assay:

    Article Title: RORγt inhibition selectively targets IL-17 producing iNKT and γδ-T cells enriched in Spondyloarthritis patients
    Article Snippet: FcR Blocking Reagent (Miltenyi Biotec) was used to exclude aspecific FcR binding of Abs. .. For cytokine stainings, cells were collected and restimulated for 4 h with PMA (25 ng/mL), calcium-ionomycin (1 µg/mL; both from Sigma) and brefeldin A (10 ng/mL; BD).

    CTL Assay:

    Article Title: Amniotic mesenchymal cells from pre‐eclamptic placentae maintain immunomodulatory features as healthy controls
    Article Snippet: Analysis of different T‐cell subsets The phenotypes of different T‐cell subsets derived from MLC experiments were assessed after 6 days of co‐culturing by FACS analysis using a set of cell surface markers and intracellular cytokines to characterize CD4+ T helper (Th) cells Th1 , , Th2 , , Th17 , , Treg and CD8+ cytotoxic T lymphocytes (CTL) , as reported in Table . .. Therefore, cells were stimulated for 5 hrs at 37°C with phorbol‐12‐myristate‐13‐acetate (50 ng/ml), calcium‐ionomycin (CaI, 1 μg/ml) and Brefeldin A (10 μg/ml) (all from Sigma‐Aldrich), and viable cells were analysed on a flow cytometer.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Altered thymic differentiation and modulation of arthritis by invariant NKT cells expressing mutant ZAP70
    Article Snippet: For evaluation of IFN-γ production by iNKT cells directly ex vivo, freshly isolated PBMC and SFMC were incubated for 4 h with phorbol 12-myristate 13-acetate (PMA, 25 ng/ml), calcium-ionomycin (CaI, 1 µg/ml; both from Sigma) and brefeldin A (BFA, 10 ng/ml; BD) or BFA alone (negative control). .. In separate experiments, 3 × 106 PBMC and SFMC were incubated for 48 h with αGC (100 ng/ml; Laboratory for Medicinal Chemistry, Ghent University) and IFN-γ levels were measured in supernatants of PB and SF cell cultures by means of ELISA (eBioscience).

    Incubation:

    Article Title: RORγt inhibition selectively targets IL-17 producing iNKT and γδ-T cells enriched in Spondyloarthritis patients
    Article Snippet: For flowcytometric analyses of cultured cells, Ab staining was preceded by incubation with viable cell discriminating reagents (LIVE/DEAD® Fixable Violet Dead Cell Stain Kit, ThermoFisher or Fixable Viability Stain 520, BD). .. For cytokine stainings, cells were collected and restimulated for 4 h with PMA (25 ng/mL), calcium-ionomycin (1 µg/mL; both from Sigma) and brefeldin A (10 ng/mL; BD).

    Article Title: Separately or Combined, LukG/LukH Is Functionally Unique Compared to Other Staphylococcal Bicomponent Leukotoxins
    Article Snippet: The leukocyte-rich plasma was separated after 20 min incubation at room temperature and cells were pelleted by centrifugation at 1000 rpm for 10 min at 5°C. .. To examine the role of calcium channels in calcium ion entry, in a set of experiments, methoxyverapamil (Sigma) was added at 100 µM or 400 µM 60 min prior to addition of BCL components.

    Article Title: Astacin Proteases Cleave Dentin Sialophosphoprotein (Dspp) to Generate Dentin Phosphoprotein (Dpp)
    Article Snippet: Two different concentrations of Dpp (1.5 mg/1.5 mL or 1.5 mg/0.5 mL) were incubated with 0.5 µg of rhMEP1B in HEPES buffer (described earlier) at 37°C for 20 hours, respectively. .. To determine the calcium ion concentration, the digests were passed through a Microcon 3 filter (cutoff = 3,500 Da; Millipore, Billerica, MA, USA).

    Article Title: Altered thymic differentiation and modulation of arthritis by invariant NKT cells expressing mutant ZAP70
    Article Snippet: .. For evaluation of IFN-γ production by iNKT cells directly ex vivo, freshly isolated PBMC and SFMC were incubated for 4 h with phorbol 12-myristate 13-acetate (PMA, 25 ng/ml), calcium-ionomycin (CaI, 1 µg/ml; both from Sigma) and brefeldin A (BFA, 10 ng/ml; BD) or BFA alone (negative control). .. Cells were prepared for intracellular cytokine staining as described before using the Cytofix/Cytoperm kit (BD), L/D Fixable Violet Dead Cell Stain Kit (ThermoFisher) and anti-human IFN-γ PERCP-Cy5.5 mAb (45.B3, eBioscience).

    Article Title: CGA-N12, a peptide derived from chromogranin A, promotes apoptosis of Candida tropicalis by attenuating mitochondrial functions
    Article Snippet: Detection of calcium ion uptake To elucidate Ca2+ uptake under the effect of CGA-N12, Ca2+ probes Fura-2-AM and Rhod-2-AM (Sigma–Aldrich, Shanghai, China) were used to detect changes in the Ca2+ content of the cytosol and mitochondria, respectively [ ]. .. C. tropicalis cells (1 × 106 ) were incubated with 75 µM CGA-N12 for 0, 5, 10 and 15 h at 28°C; 10 mM H2 O2 was used as a positive control.

    Article Title: Effects of 3D culturing conditions on the transcriptomic profile of stem-cell-derived neurons
    Article Snippet: Before encapsulation experiments, the following preparations were done: GFR Matrigel was kept on ice, sodium alginate (PRONOVA UP VLVG, NovaMatrix) was first reconstituted at 4% (40 mg/ml) in a medium made of 1:3 mixture of mTeSR and neurobasal medium (without supplements) with 150 mM sodium chloride (NaCl) (Sigma) and this solution was then incubated for 6 hours at 37 °C for further dissolution of alginate. .. Calcium chloride (CaCl2 ) solution (1M in water, Sigma) was diluted in a medium made of 1:3 mixture of mTeSR and neurobasal medium (without supplements) at concentrations of 25 mM, 12.5 mM, 6.25 mM and 3.125 mM.

    Diffusion-based Assay:

    Article Title: A novel self-assembled oligopeptide amphiphile for biomimetic mineralization of enamel
    Article Snippet: A 100 μL aliquot of a 1 M calcium chloride (CaCl2 ) solution (Sigma-Aldrich, St. Louis, MO, USA) was added to 1 mL of the oligopeptide solutions at concentrations of 1, 2.5, 5.0, or 10.0 mg/mL. .. A suspension was formed and mixed thoroughly in a vial after each drop of the CaCl2 solution to promote diffusion of the metal ions.

    Expressing:

    Article Title: RORγt inhibition selectively targets IL-17 producing iNKT and γδ-T cells enriched in Spondyloarthritis patients
    Article Snippet: A minimum of 500 iNKT and 3000 γδ-T cells were acquired on a FACS Canto II or Aria III machine (BD) to guarantee accurate calculations of the expression of differentiation markers/transcription factors by these cells. .. For cytokine stainings, cells were collected and restimulated for 4 h with PMA (25 ng/mL), calcium-ionomycin (1 µg/mL; both from Sigma) and brefeldin A (10 ng/mL; BD).

    Ex Vivo:

    Article Title: Altered thymic differentiation and modulation of arthritis by invariant NKT cells expressing mutant ZAP70
    Article Snippet: .. For evaluation of IFN-γ production by iNKT cells directly ex vivo, freshly isolated PBMC and SFMC were incubated for 4 h with phorbol 12-myristate 13-acetate (PMA, 25 ng/ml), calcium-ionomycin (CaI, 1 µg/ml; both from Sigma) and brefeldin A (BFA, 10 ng/ml; BD) or BFA alone (negative control). .. Cells were prepared for intracellular cytokine staining as described before using the Cytofix/Cytoperm kit (BD), L/D Fixable Violet Dead Cell Stain Kit (ThermoFisher) and anti-human IFN-γ PERCP-Cy5.5 mAb (45.B3, eBioscience).

    Recombinase Polymerase Amplification:

    Article Title: PD-L1 is a critical mediator of regulatory B cells and T cells in invasive breast cancer
    Article Snippet: The following antibodies for Fluorescence Minus One (FMO): CD3-FITC (clone: BW264/56, Miltenyi Biotec, Germany), PD-L1-PE (clone: PD1.3.1.3), CD19-ECD (clone: J3-119), CD4-APC (clone: RPA-T4), CD14-PE 770 (clone: TUK4, Miltenyi Biotec, Germany). .. Lipopolysaccharide, PMA and calcium-ionomycin were obtained from Sigma (St Louis, USA).

    Derivative Assay:

    Article Title: Amniotic mesenchymal cells from pre‐eclamptic placentae maintain immunomodulatory features as healthy controls
    Article Snippet: Analysis of different T‐cell subsets The phenotypes of different T‐cell subsets derived from MLC experiments were assessed after 6 days of co‐culturing by FACS analysis using a set of cell surface markers and intracellular cytokines to characterize CD4+ T helper (Th) cells Th1 , , Th2 , , Th17 , , Treg and CD8+ cytotoxic T lymphocytes (CTL) , as reported in Table . .. Therefore, cells were stimulated for 5 hrs at 37°C with phorbol‐12‐myristate‐13‐acetate (50 ng/ml), calcium‐ionomycin (CaI, 1 μg/ml) and Brefeldin A (10 μg/ml) (all from Sigma‐Aldrich), and viable cells were analysed on a flow cytometer.

    Electron Microscopy:

    Article Title: A novel self-assembled oligopeptide amphiphile for biomimetic mineralization of enamel
    Article Snippet: A 100 μL aliquot of a 1 M calcium chloride (CaCl2 ) solution (Sigma-Aldrich, St. Louis, MO, USA) was added to 1 mL of the oligopeptide solutions at concentrations of 1, 2.5, 5.0, or 10.0 mg/mL. .. The self-assembled oligopeptide was characterized by transmission electron microscopy (TEM) and scanning electron microscopy (SEM) as the following.

    Flow Cytometry:

    Article Title: RORγt inhibition selectively targets IL-17 producing iNKT and γδ-T cells enriched in Spondyloarthritis patients
    Article Snippet: Paragraph title: Flow cytometry ... For cytokine stainings, cells were collected and restimulated for 4 h with PMA (25 ng/mL), calcium-ionomycin (1 µg/mL; both from Sigma) and brefeldin A (10 ng/mL; BD).

    Article Title: Amniotic mesenchymal cells from pre‐eclamptic placentae maintain immunomodulatory features as healthy controls
    Article Snippet: .. Therefore, cells were stimulated for 5 hrs at 37°C with phorbol‐12‐myristate‐13‐acetate (50 ng/ml), calcium‐ionomycin (CaI, 1 μg/ml) and Brefeldin A (10 μg/ml) (all from Sigma‐Aldrich), and viable cells were analysed on a flow cytometer. ..

    Article Title: Altered thymic differentiation and modulation of arthritis by invariant NKT cells expressing mutant ZAP70
    Article Snippet: Samples were acquired on a FACS Canto II flow cytometer (BD) and analyzed by means of FlowJo 10.3 software (Treestar). .. For evaluation of IFN-γ production by iNKT cells directly ex vivo, freshly isolated PBMC and SFMC were incubated for 4 h with phorbol 12-myristate 13-acetate (PMA, 25 ng/ml), calcium-ionomycin (CaI, 1 µg/ml; both from Sigma) and brefeldin A (BFA, 10 ng/ml; BD) or BFA alone (negative control).

    Cell Culture:

    Article Title: RORγt inhibition selectively targets IL-17 producing iNKT and γδ-T cells enriched in Spondyloarthritis patients
    Article Snippet: For flowcytometric analyses of cultured cells, Ab staining was preceded by incubation with viable cell discriminating reagents (LIVE/DEAD® Fixable Violet Dead Cell Stain Kit, ThermoFisher or Fixable Viability Stain 520, BD). .. For cytokine stainings, cells were collected and restimulated for 4 h with PMA (25 ng/mL), calcium-ionomycin (1 µg/mL; both from Sigma) and brefeldin A (10 ng/mL; BD).

    Calcium Assay:

    Article Title: Sustained delivery of calcium and orthophosphate ions from amorphous calcium phosphate and poly(L-lactic acid)-based electrospinning nanofibrous scaffold
    Article Snippet: The release of calcium ion during scaffold degradation was measured with calcium colorimetric assay kit (Sigma-Aldrich, USA). .. A total of 90 μL chromogenic reagent and 60 μL calcium assay buffer were added to 96 well plates containing 50 μL supernatant.

    other:

    Article Title: Highly Efficient Non-Enzymatic Glucose Sensor Based on CuO Modified Vertically-Grown ZnO Nanorods on Electrode
    Article Snippet: Reagents Fluorine doped tin oxide (FTO) coated glass electrode, Zinc nitrate hexahydrate (Zn(NO3 )2 •6H2 O, 98%), hexamethylenetetramine (HMTA; C6 H12 N4 , ≥99.0%), copper(II) sulfate pentahydrate (CuSO4 •5H2 O, ≥98.0%), Nafion (5 wt.% in lower aliphatic alcohol and water mixture), sodium hydroxide (NaOH, ≥97.0%), D-(+)-Glucose (C6 H12 O6 , 99.5%), ascorbic acid (AA), uric acid (UA), dopamine (DA), β-Nicotinamide adenine dinucleotide (NADH), magnesium chloride (MgCl2 , ≥98%), calcium chloride (CaCl2 ) solution, L-cysteine (L-cys), sodium chloride (NaCl, ≥99.5%), lactose, sucrose, maltose, and mannose were purchased from Sigma-Aldrich.

    Transmission Assay:

    Article Title: A novel self-assembled oligopeptide amphiphile for biomimetic mineralization of enamel
    Article Snippet: A 100 μL aliquot of a 1 M calcium chloride (CaCl2 ) solution (Sigma-Aldrich, St. Louis, MO, USA) was added to 1 mL of the oligopeptide solutions at concentrations of 1, 2.5, 5.0, or 10.0 mg/mL. .. The self-assembled oligopeptide was characterized by transmission electron microscopy (TEM) and scanning electron microscopy (SEM) as the following.

    Binding Assay:

    Article Title: RORγt inhibition selectively targets IL-17 producing iNKT and γδ-T cells enriched in Spondyloarthritis patients
    Article Snippet: FcR Blocking Reagent (Miltenyi Biotec) was used to exclude aspecific FcR binding of Abs. .. For cytokine stainings, cells were collected and restimulated for 4 h with PMA (25 ng/mL), calcium-ionomycin (1 µg/mL; both from Sigma) and brefeldin A (10 ng/mL; BD).

    Molecular Weight:

    Article Title: Sustained delivery of calcium and orthophosphate ions from amorphous calcium phosphate and poly(L-lactic acid)-based electrospinning nanofibrous scaffold
    Article Snippet: The molecular weight change of PLLA was tested by GPC (UPLC/Premier, Waters, USA). .. The release of calcium ion during scaffold degradation was measured with calcium colorimetric assay kit (Sigma-Aldrich, USA).

    Fluorescence:

    Article Title: PD-L1 is a critical mediator of regulatory B cells and T cells in invasive breast cancer
    Article Snippet: The following antibodies for Fluorescence Minus One (FMO): CD3-FITC (clone: BW264/56, Miltenyi Biotec, Germany), PD-L1-PE (clone: PD1.3.1.3), CD19-ECD (clone: J3-119), CD4-APC (clone: RPA-T4), CD14-PE 770 (clone: TUK4, Miltenyi Biotec, Germany). .. Lipopolysaccharide, PMA and calcium-ionomycin were obtained from Sigma (St Louis, USA).

    Isolation:

    Article Title: PD-L1 mediated the differentiation of tumor-infiltrating CD19+ B lymphocytes and T cells in Invasive breast cancer
    Article Snippet: .. Inc. USA); CD19-ECD (Beckman Coulter Company, Marseille, France); PD-1-FITC (clone: MIH4) and PD-L1-PE (clone: 29E.2A3) were from Biolegend; CD38-APC (clone: HIT2), CD24-PE-cyanire7 (clone: eBioSN3), CD25-PE-cy7 (clone: BC96), IL-10-FITC (clone: BT-10) and anti-Foxp3-APC (clone: PCH101) were purchased from eBiosciences (San Jose, CA, USA); Anti-CD3e monoclonal antibody (clone: OKT3, Glucan Bio-tech., Changzhou, China); Lipopolysaccharide (Sigma, USA); PMA(Sigma, USA); calcium-ionomycin (Sigma, USA); Foxp3 Fixation /Permeabilization Solution (Biolegend, USA); Cell Fixation/Permeabilization Kits for Intracellular Cytokine Analysis (BD Biosciences, USA); CD4+ T cell and CD19+ B cell positive isolation kit (Miltenyi Biotec, Germany); ChemMate™ Envision/HRP technique (Gene Tech, Shanghai, China). .. Alexa Fluor 488 donkey anti-Mouse IgG (H+ L) antibody (Invitrogen, USA); Cy™3-conjugated donkey anti-Rabbit IgG (H+ L) antibody (Jackson ImmunoResearch Laboratories, Inc.).

    Article Title: Separately or Combined, LukG/LukH Is Functionally Unique Compared to Other Staphylococcal Bicomponent Leukotoxins
    Article Snippet: Resuspended cells in 0.9% NaCl were layered over 10 mL Ficoll-Paque (GE Healthcare) and then spun at 1400 rpm at 20°C for 40 min. PMNs were isolated by centrifugation at 1000 rpm at 5°C for 6 min after lysing RBCs by 0.2% NaCl and 1.6% NaCl. .. To examine the role of calcium channels in calcium ion entry, in a set of experiments, methoxyverapamil (Sigma) was added at 100 µM or 400 µM 60 min prior to addition of BCL components.

    Article Title: Altered thymic differentiation and modulation of arthritis by invariant NKT cells expressing mutant ZAP70
    Article Snippet: .. For evaluation of IFN-γ production by iNKT cells directly ex vivo, freshly isolated PBMC and SFMC were incubated for 4 h with phorbol 12-myristate 13-acetate (PMA, 25 ng/ml), calcium-ionomycin (CaI, 1 µg/ml; both from Sigma) and brefeldin A (BFA, 10 ng/ml; BD) or BFA alone (negative control). .. Cells were prepared for intracellular cytokine staining as described before using the Cytofix/Cytoperm kit (BD), L/D Fixable Violet Dead Cell Stain Kit (ThermoFisher) and anti-human IFN-γ PERCP-Cy5.5 mAb (45.B3, eBioscience).

    Purification:

    Article Title: Lipid Bilayers Are Long-Lived on Solvent Cleaned Plasma-Oxidized poly(dimethyl)siloxane (ox-PDMS)
    Article Snippet: Chloroform (anhydrous, ≥ 99% contains 0.5–1.0% ethanol as stabilizer), 1.0 M calcium chloride (CaCl2 ) solution (BioUltra, for molecular biology) were purchased from Sigma Aldrich (Missouri, MO). .. Ultrapure water (18.2 MΩ cm resistivity) was obtained from an ELGA Purelab Ultra water purification system (Woodridge, IL).1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) (ammonium salt) (Rh-DPPE) were all purchased from Avanti Polar Phospholipids (Alabaster, AL).

    Article Title: Separately or Combined, LukG/LukH Is Functionally Unique Compared to Other Staphylococcal Bicomponent Leukotoxins
    Article Snippet: Purified PMNs were suspended in Hank’s balanced salt solution (HBSS). .. To examine the role of calcium channels in calcium ion entry, in a set of experiments, methoxyverapamil (Sigma) was added at 100 µM or 400 µM 60 min prior to addition of BCL components.

    Article Title: Astacin Proteases Cleave Dentin Sialophosphoprotein (Dspp) to Generate Dentin Phosphoprotein (Dpp)
    Article Snippet: Digestion of Dpp by proteinases Purified Dpp (30 µg) was incubated for 20 hours in 25 mM HEPES, 2 mM CaCl2 , 1 mM ZnCl2 , and 0.01% Brij 35 buffer (pH 7.5) at 37°C with 0.5 µg of rhMMP-2, rhMMP-8, rhMMP-9, rhMT1-MMP, rhMT3-MMP, rhBMP-1, rhMEP1A, or rhMEP1B, with 10 µg of porcine Klk4 or porcine MMP-20, or with 0.1 unit of human placental plasmin. .. To determine the calcium ion concentration, the digests were passed through a Microcon 3 filter (cutoff = 3,500 Da; Millipore, Billerica, MA, USA).

    Diff-Quik:

    Article Title: Separately or Combined, LukG/LukH Is Functionally Unique Compared to Other Staphylococcal Bicomponent Leukotoxins
    Article Snippet: PMN purity ( > 95%) was confirmed with Diff Quick (Thermo Scientific) staining. .. To examine the role of calcium channels in calcium ion entry, in a set of experiments, methoxyverapamil (Sigma) was added at 100 µM or 400 µM 60 min prior to addition of BCL components.

    FACS:

    Article Title: RORγt inhibition selectively targets IL-17 producing iNKT and γδ-T cells enriched in Spondyloarthritis patients
    Article Snippet: A minimum of 500 iNKT and 3000 γδ-T cells were acquired on a FACS Canto II or Aria III machine (BD) to guarantee accurate calculations of the expression of differentiation markers/transcription factors by these cells. .. For cytokine stainings, cells were collected and restimulated for 4 h with PMA (25 ng/mL), calcium-ionomycin (1 µg/mL; both from Sigma) and brefeldin A (10 ng/mL; BD).

    Article Title: Amniotic mesenchymal cells from pre‐eclamptic placentae maintain immunomodulatory features as healthy controls
    Article Snippet: Analysis of different T‐cell subsets The phenotypes of different T‐cell subsets derived from MLC experiments were assessed after 6 days of co‐culturing by FACS analysis using a set of cell surface markers and intracellular cytokines to characterize CD4+ T helper (Th) cells Th1 , , Th2 , , Th17 , , Treg and CD8+ cytotoxic T lymphocytes (CTL) , as reported in Table . .. Therefore, cells were stimulated for 5 hrs at 37°C with phorbol‐12‐myristate‐13‐acetate (50 ng/ml), calcium‐ionomycin (CaI, 1 μg/ml) and Brefeldin A (10 μg/ml) (all from Sigma‐Aldrich), and viable cells were analysed on a flow cytometer.

    Article Title: Altered thymic differentiation and modulation of arthritis by invariant NKT cells expressing mutant ZAP70
    Article Snippet: Samples were acquired on a FACS Canto II flow cytometer (BD) and analyzed by means of FlowJo 10.3 software (Treestar). .. For evaluation of IFN-γ production by iNKT cells directly ex vivo, freshly isolated PBMC and SFMC were incubated for 4 h with phorbol 12-myristate 13-acetate (PMA, 25 ng/ml), calcium-ionomycin (CaI, 1 µg/ml; both from Sigma) and brefeldin A (BFA, 10 ng/ml; BD) or BFA alone (negative control).

    Software:

    Article Title: RORγt inhibition selectively targets IL-17 producing iNKT and γδ-T cells enriched in Spondyloarthritis patients
    Article Snippet: Data analyses and graph presentations were done by means of FlowJo software, v9.8 and 10.2 (Treestar). .. For cytokine stainings, cells were collected and restimulated for 4 h with PMA (25 ng/mL), calcium-ionomycin (1 µg/mL; both from Sigma) and brefeldin A (10 ng/mL; BD).

    Article Title: Altered thymic differentiation and modulation of arthritis by invariant NKT cells expressing mutant ZAP70
    Article Snippet: Samples were acquired on a FACS Canto II flow cytometer (BD) and analyzed by means of FlowJo 10.3 software (Treestar). .. For evaluation of IFN-γ production by iNKT cells directly ex vivo, freshly isolated PBMC and SFMC were incubated for 4 h with phorbol 12-myristate 13-acetate (PMA, 25 ng/ml), calcium-ionomycin (CaI, 1 µg/ml; both from Sigma) and brefeldin A (BFA, 10 ng/ml; BD) or BFA alone (negative control).

    Negative Control:

    Article Title: Altered thymic differentiation and modulation of arthritis by invariant NKT cells expressing mutant ZAP70
    Article Snippet: .. For evaluation of IFN-γ production by iNKT cells directly ex vivo, freshly isolated PBMC and SFMC were incubated for 4 h with phorbol 12-myristate 13-acetate (PMA, 25 ng/ml), calcium-ionomycin (CaI, 1 µg/ml; both from Sigma) and brefeldin A (BFA, 10 ng/ml; BD) or BFA alone (negative control). .. Cells were prepared for intracellular cytokine staining as described before using the Cytofix/Cytoperm kit (BD), L/D Fixable Violet Dead Cell Stain Kit (ThermoFisher) and anti-human IFN-γ PERCP-Cy5.5 mAb (45.B3, eBioscience).

    In Vitro:

    Article Title: Sustained delivery of calcium and orthophosphate ions from amorphous calcium phosphate and poly(L-lactic acid)-based electrospinning nanofibrous scaffold
    Article Snippet: Paragraph title: In vitro hydrolytic degradation ... The release of calcium ion during scaffold degradation was measured with calcium colorimetric assay kit (Sigma-Aldrich, USA).

    Gel Permeation Chromatography:

    Article Title: Sustained delivery of calcium and orthophosphate ions from amorphous calcium phosphate and poly(L-lactic acid)-based electrospinning nanofibrous scaffold
    Article Snippet: The molecular weight change of PLLA was tested by GPC (UPLC/Premier, Waters, USA). .. The release of calcium ion during scaffold degradation was measured with calcium colorimetric assay kit (Sigma-Aldrich, USA).

    Concentration Assay:

    Article Title: Sustained delivery of calcium and orthophosphate ions from amorphous calcium phosphate and poly(L-lactic acid)-based electrospinning nanofibrous scaffold
    Article Snippet: The release of calcium ion during scaffold degradation was measured with calcium colorimetric assay kit (Sigma-Aldrich, USA). .. In this assay, the calcium ion concentration was determined by the chromogenic complex formed by calcium ion and o -cresolphthalein, which was proportional to the concentration of calcium ion.

    Article Title: Astacin Proteases Cleave Dentin Sialophosphoprotein (Dspp) to Generate Dentin Phosphoprotein (Dpp)
    Article Snippet: .. To determine the calcium ion concentration, the digests were passed through a Microcon 3 filter (cutoff = 3,500 Da; Millipore, Billerica, MA, USA). ..

    Article Title: Effects of 3D culturing conditions on the transcriptomic profile of stem-cell-derived neurons
    Article Snippet: Calcium chloride (CaCl2 ) solution (1M in water, Sigma) was diluted in a medium made of 1:3 mixture of mTeSR and neurobasal medium (without supplements) at concentrations of 25 mM, 12.5 mM, 6.25 mM and 3.125 mM. .. HA sodium salt (Sigma, #53747) was dissolved at 1% (10 mg/ml) concentration under sterile conditions in a medium made of 1:3 mixture of mTeSR and neurobasal medium (without supplements) and then incubated for 6 hours at 37 °C for further dissolution of HA and frequently vortexed.

    Transmission Electron Microscopy:

    Article Title: A novel self-assembled oligopeptide amphiphile for biomimetic mineralization of enamel
    Article Snippet: A 100 μL aliquot of a 1 M calcium chloride (CaCl2 ) solution (Sigma-Aldrich, St. Louis, MO, USA) was added to 1 mL of the oligopeptide solutions at concentrations of 1, 2.5, 5.0, or 10.0 mg/mL. .. The self-assembled oligopeptide was characterized by transmission electron microscopy (TEM) and scanning electron microscopy (SEM) as the following.

    Calcium Colorimetric Assay:

    Article Title: Sustained delivery of calcium and orthophosphate ions from amorphous calcium phosphate and poly(L-lactic acid)-based electrospinning nanofibrous scaffold
    Article Snippet: .. The release of calcium ion during scaffold degradation was measured with calcium colorimetric assay kit (Sigma-Aldrich, USA). .. In this assay, the calcium ion concentration was determined by the chromogenic complex formed by calcium ion and o -cresolphthalein, which was proportional to the concentration of calcium ion.

    Staining:

    Article Title: RORγt inhibition selectively targets IL-17 producing iNKT and γδ-T cells enriched in Spondyloarthritis patients
    Article Snippet: Prior to intracellular staining of transcription factors, the cells were fixed and permeabilized using the reagents and following the instructions of the FoxP3 intracellular staining kit (eBioscience). .. For cytokine stainings, cells were collected and restimulated for 4 h with PMA (25 ng/mL), calcium-ionomycin (1 µg/mL; both from Sigma) and brefeldin A (10 ng/mL; BD).

    Article Title: Separately or Combined, LukG/LukH Is Functionally Unique Compared to Other Staphylococcal Bicomponent Leukotoxins
    Article Snippet: PMN purity ( > 95%) was confirmed with Diff Quick (Thermo Scientific) staining. .. To examine the role of calcium channels in calcium ion entry, in a set of experiments, methoxyverapamil (Sigma) was added at 100 µM or 400 µM 60 min prior to addition of BCL components.

    Article Title: Altered thymic differentiation and modulation of arthritis by invariant NKT cells expressing mutant ZAP70
    Article Snippet: Blood and SF samples were diluted in RPMI medium and mononuclear cells (MC) were isolated by density gradient centrifugation using histopaque®-1077 (Sigma-Aldrich). iNKT cells were defined as live CD3+ CD19− TCRVα24Jα18+ TCRVβ11+ cells detected within the lymphocyte gate of PBMC and SFMC samples using the L/D stain 7-AAD (BD) and the following monoclonal antibodies: anti-human CD3 APC-eFluor780 (UCHT1), CD19 PERCP-Cy5.5 (HIB19), TCRVα24Jα18 PE (6B11, all from eBiosciences) and TCRVβ11 FITC (C21, Beckman Coulter). .. For evaluation of IFN-γ production by iNKT cells directly ex vivo, freshly isolated PBMC and SFMC were incubated for 4 h with phorbol 12-myristate 13-acetate (PMA, 25 ng/ml), calcium-ionomycin (CaI, 1 µg/ml; both from Sigma) and brefeldin A (BFA, 10 ng/ml; BD) or BFA alone (negative control).

    Gradient Centrifugation:

    Article Title: Altered thymic differentiation and modulation of arthritis by invariant NKT cells expressing mutant ZAP70
    Article Snippet: Blood and SF samples were diluted in RPMI medium and mononuclear cells (MC) were isolated by density gradient centrifugation using histopaque®-1077 (Sigma-Aldrich). iNKT cells were defined as live CD3+ CD19− TCRVα24Jα18+ TCRVβ11+ cells detected within the lymphocyte gate of PBMC and SFMC samples using the L/D stain 7-AAD (BD) and the following monoclonal antibodies: anti-human CD3 APC-eFluor780 (UCHT1), CD19 PERCP-Cy5.5 (HIB19), TCRVα24Jα18 PE (6B11, all from eBiosciences) and TCRVβ11 FITC (C21, Beckman Coulter). .. For evaluation of IFN-γ production by iNKT cells directly ex vivo, freshly isolated PBMC and SFMC were incubated for 4 h with phorbol 12-myristate 13-acetate (PMA, 25 ng/ml), calcium-ionomycin (CaI, 1 µg/ml; both from Sigma) and brefeldin A (BFA, 10 ng/ml; BD) or BFA alone (negative control).

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