calcein acetoxymethylester  (Millipore)


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    Name:
    Calcein AM
    Description:
    Liposomes contain a self quenching fluorescent dye called calcein It is a fluorescent dye with excitation and emission wavelengths of 495 515 nm respectively It self quenches at concentrations above 70mM and is commonly used as an indicator of lipid vesicle leakage The leakage rate is calculated on the basis of the fluorescence increment as the entrapped calcein is leaked out of the liposomal compartment It is used as a complexometric indicator for titration of calcium ions with EDTA and for fluorometric determination of calcium
    Catalog Number:
    17783
    Price:
    None
    Applications:
    Non-fluorescent cell permeable derivative of calcein, becomes fluorescent on hydrolysis. Used as a neutral substrate for multidrug resistance protein, MRP; employed in tumor research.
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    Structured Review

    Millipore calcein acetoxymethylester
    Calcein AM
    Liposomes contain a self quenching fluorescent dye called calcein It is a fluorescent dye with excitation and emission wavelengths of 495 515 nm respectively It self quenches at concentrations above 70mM and is commonly used as an indicator of lipid vesicle leakage The leakage rate is calculated on the basis of the fluorescence increment as the entrapped calcein is leaked out of the liposomal compartment It is used as a complexometric indicator for titration of calcium ions with EDTA and for fluorometric determination of calcium
    https://www.bioz.com/result/calcein acetoxymethylester/product/Millipore
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    calcein acetoxymethylester - by Bioz Stars, 2020-08
    99/100 stars

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    Related Articles

    Synthesized:

    Article Title: ROS-mediated iron overload injures the hematopoiesis of bone marrow by damaging hematopoietic stem/progenitor cells in mice
    Article Snippet: .. Reagents Anti-mouse-CD45.1-FITC, CD45.2-PE, Gr-1-PE/CY7, CD45R/B220-PE/CY5.5 CD11b-PE/CY7 and CD3-APCwere purchased from BioLegend (San Diego, CA, USA); anti-mouse-Sca-1-PE, CD117 (c-kit) Alexa Fluor 700, CD4, CD8, CD45R/B220, Gr-1, CD11b, Ter119 and APC/CY7-conjugated streptavidin were purchased from eBioscience (San Diego, CA, USA); iron-dextrin was purchased from Pharmacosmos A/S (Denmark); the ROS staining kit (S0033) and NAC were purchased from the Beyotime Institute of Biotechnology; calcein-AM fluorescent dye was purchased from Sigma-Aldrich (USA); deferasirox was purchased from Novartis; RPMI 1640 was purchased from Gibco (USA); methylcellulose M3434 was purchased from Stem cell (USA); fetal calf serum was purchased from Bioind (Italy); CD117 MicroBeads (130-091-229) were purchased from Miltenyi Biotec (Germany); a RNA PCR Kit (AMV) Ver.3.0 (DRR019A) was purchased from Takara; a RNeasy MicroKit (74004) was purchased from Qiagen (Germany); the NOX4 and GPX1 gene qRT-PCR primers were synthesized by Sangon Biotech (Shanghai, China). .. Animals and treatments Male C57BL/6-Ly-5.1 (Ly45.1) and C57BL/6-Ly5.2 (Ly45.2) mice were purchased from the Institute of Laboratory Animal Sciences (PUMC, Beijing, China) and from Vital River (Beijing, China).

    Infection:

    Article Title: Distinct efficacy of HIV-1 entry inhibitors to prevent cell-to-cell transfer of R5 and X4 viruses across a human placental trophoblast barrier in a reconstitution model in vitro
    Article Snippet: .. Human PBMCs, at day-7 post infection with HIV-1 LAI, were labelled with the green cytoplasmic dye calcein-AM (Sigma-Aldrich) at 1.5 μg/ml in PBS. ..

    Concentration Assay:

    Article Title: MiRNA-296-3p-ICAM-1 axis promotes metastasis of prostate cancer by possible enhancing survival of natural killer cell-resistant circulating tumour cells
    Article Snippet: .. The target cells were labelled with calcein-AM (Sigma-Aldrich) 3 μ g/ml at a concentration of 1 × 106 cells/ml at 37 °C for 30 min. .. If target cells were transfected by lentivirus and expressed GFP, this step should be omitted.

    Article Title: Human Immunodeficiency Virus Protease Inhibitors Serve as Substrates for Multidrug Transporter Proteins MDR1 and MRP1 but Retain Antiviral Efficacy in Cell Lines Expressing These Transporters
    Article Snippet: .. After a 2-min incubation, calcein-AM was added to a final concentration of 1 μM and the plates were placed in a cytofluorimeter (Millipore, Bedford, Mass.). .. Fluorescence was measured at regular intervals for 30 to 60 min with 485-nm (bandwidth, 20 nm) excitation and 530-nm (bandwidth, 25 nm) emission filters.

    Article Title: Effects of FTMT Expression by Retinal Pigment Epithelial Cells on Features of Angiogenesis
    Article Snippet: .. Cells were labeled with calcein-AM (Sigma-Aldrich) at a final concentration of 2 μg/mL for 30 min. .. Cells were removed from culture vessel with 0.125% Trypsin/1 mM EDTA, centrifuged at 200 g/3 min, and resuspended in DMEM/0.2% FBS.

    Incubation:

    Article Title: Human Immunodeficiency Virus Protease Inhibitors Serve as Substrates for Multidrug Transporter Proteins MDR1 and MRP1 but Retain Antiviral Efficacy in Cell Lines Expressing These Transporters
    Article Snippet: .. After a 2-min incubation, calcein-AM was added to a final concentration of 1 μM and the plates were placed in a cytofluorimeter (Millipore, Bedford, Mass.). .. Fluorescence was measured at regular intervals for 30 to 60 min with 485-nm (bandwidth, 20 nm) excitation and 530-nm (bandwidth, 25 nm) emission filters.

    Article Title: Senescent tumor cells lead the collective invasion in thyroid cancer
    Article Snippet: .. Cell detachment induced apoptosis was analysed by Calcein AM/EthD-1 staining (Sigma) after 12 h incubation. .. Immunoblotting Cells were lysed in RIPA buffer (Tris pH 7.5, 20 mM; NaCl,150 mM; 1% Nonidet P-40; 0.5% sodium deoxycholate; EDTA,1 mM; 0.1% SDS) containing protease inhibitor cocktail (K272, Biovision, Milpitas, CA, USA) and phosphatase inhibitor cocktail (K282, Biovision).

    Labeling:

    Article Title: Effects of FTMT Expression by Retinal Pigment Epithelial Cells on Features of Angiogenesis
    Article Snippet: .. Cells were labeled with calcein-AM (Sigma-Aldrich) at a final concentration of 2 μg/mL for 30 min. .. Cells were removed from culture vessel with 0.125% Trypsin/1 mM EDTA, centrifuged at 200 g/3 min, and resuspended in DMEM/0.2% FBS.

    Quantitative RT-PCR:

    Article Title: ROS-mediated iron overload injures the hematopoiesis of bone marrow by damaging hematopoietic stem/progenitor cells in mice
    Article Snippet: .. Reagents Anti-mouse-CD45.1-FITC, CD45.2-PE, Gr-1-PE/CY7, CD45R/B220-PE/CY5.5 CD11b-PE/CY7 and CD3-APCwere purchased from BioLegend (San Diego, CA, USA); anti-mouse-Sca-1-PE, CD117 (c-kit) Alexa Fluor 700, CD4, CD8, CD45R/B220, Gr-1, CD11b, Ter119 and APC/CY7-conjugated streptavidin were purchased from eBioscience (San Diego, CA, USA); iron-dextrin was purchased from Pharmacosmos A/S (Denmark); the ROS staining kit (S0033) and NAC were purchased from the Beyotime Institute of Biotechnology; calcein-AM fluorescent dye was purchased from Sigma-Aldrich (USA); deferasirox was purchased from Novartis; RPMI 1640 was purchased from Gibco (USA); methylcellulose M3434 was purchased from Stem cell (USA); fetal calf serum was purchased from Bioind (Italy); CD117 MicroBeads (130-091-229) were purchased from Miltenyi Biotec (Germany); a RNA PCR Kit (AMV) Ver.3.0 (DRR019A) was purchased from Takara; a RNeasy MicroKit (74004) was purchased from Qiagen (Germany); the NOX4 and GPX1 gene qRT-PCR primers were synthesized by Sangon Biotech (Shanghai, China). .. Animals and treatments Male C57BL/6-Ly-5.1 (Ly45.1) and C57BL/6-Ly5.2 (Ly45.2) mice were purchased from the Institute of Laboratory Animal Sciences (PUMC, Beijing, China) and from Vital River (Beijing, China).

    Polymerase Chain Reaction:

    Article Title: ROS-mediated iron overload injures the hematopoiesis of bone marrow by damaging hematopoietic stem/progenitor cells in mice
    Article Snippet: .. Reagents Anti-mouse-CD45.1-FITC, CD45.2-PE, Gr-1-PE/CY7, CD45R/B220-PE/CY5.5 CD11b-PE/CY7 and CD3-APCwere purchased from BioLegend (San Diego, CA, USA); anti-mouse-Sca-1-PE, CD117 (c-kit) Alexa Fluor 700, CD4, CD8, CD45R/B220, Gr-1, CD11b, Ter119 and APC/CY7-conjugated streptavidin were purchased from eBioscience (San Diego, CA, USA); iron-dextrin was purchased from Pharmacosmos A/S (Denmark); the ROS staining kit (S0033) and NAC were purchased from the Beyotime Institute of Biotechnology; calcein-AM fluorescent dye was purchased from Sigma-Aldrich (USA); deferasirox was purchased from Novartis; RPMI 1640 was purchased from Gibco (USA); methylcellulose M3434 was purchased from Stem cell (USA); fetal calf serum was purchased from Bioind (Italy); CD117 MicroBeads (130-091-229) were purchased from Miltenyi Biotec (Germany); a RNA PCR Kit (AMV) Ver.3.0 (DRR019A) was purchased from Takara; a RNeasy MicroKit (74004) was purchased from Qiagen (Germany); the NOX4 and GPX1 gene qRT-PCR primers were synthesized by Sangon Biotech (Shanghai, China). .. Animals and treatments Male C57BL/6-Ly-5.1 (Ly45.1) and C57BL/6-Ly5.2 (Ly45.2) mice were purchased from the Institute of Laboratory Animal Sciences (PUMC, Beijing, China) and from Vital River (Beijing, China).

    Staining:

    Article Title: ROS-mediated iron overload injures the hematopoiesis of bone marrow by damaging hematopoietic stem/progenitor cells in mice
    Article Snippet: .. Reagents Anti-mouse-CD45.1-FITC, CD45.2-PE, Gr-1-PE/CY7, CD45R/B220-PE/CY5.5 CD11b-PE/CY7 and CD3-APCwere purchased from BioLegend (San Diego, CA, USA); anti-mouse-Sca-1-PE, CD117 (c-kit) Alexa Fluor 700, CD4, CD8, CD45R/B220, Gr-1, CD11b, Ter119 and APC/CY7-conjugated streptavidin were purchased from eBioscience (San Diego, CA, USA); iron-dextrin was purchased from Pharmacosmos A/S (Denmark); the ROS staining kit (S0033) and NAC were purchased from the Beyotime Institute of Biotechnology; calcein-AM fluorescent dye was purchased from Sigma-Aldrich (USA); deferasirox was purchased from Novartis; RPMI 1640 was purchased from Gibco (USA); methylcellulose M3434 was purchased from Stem cell (USA); fetal calf serum was purchased from Bioind (Italy); CD117 MicroBeads (130-091-229) were purchased from Miltenyi Biotec (Germany); a RNA PCR Kit (AMV) Ver.3.0 (DRR019A) was purchased from Takara; a RNeasy MicroKit (74004) was purchased from Qiagen (Germany); the NOX4 and GPX1 gene qRT-PCR primers were synthesized by Sangon Biotech (Shanghai, China). .. Animals and treatments Male C57BL/6-Ly-5.1 (Ly45.1) and C57BL/6-Ly5.2 (Ly45.2) mice were purchased from the Institute of Laboratory Animal Sciences (PUMC, Beijing, China) and from Vital River (Beijing, China).

    Article Title: Senescent tumor cells lead the collective invasion in thyroid cancer
    Article Snippet: .. Cell detachment induced apoptosis was analysed by Calcein AM/EthD-1 staining (Sigma) after 12 h incubation. .. Immunoblotting Cells were lysed in RIPA buffer (Tris pH 7.5, 20 mM; NaCl,150 mM; 1% Nonidet P-40; 0.5% sodium deoxycholate; EDTA,1 mM; 0.1% SDS) containing protease inhibitor cocktail (K272, Biovision, Milpitas, CA, USA) and phosphatase inhibitor cocktail (K282, Biovision).

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  • 99
    Millipore calcein acetoxymethylester
    Calcein Acetoxymethylester, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/calcein acetoxymethylester/product/Millipore
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    calcein acetoxymethylester - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    99
    Millipore calcein acetoxymethylester calcein am
    Calcein Acetoxymethylester Calcein Am, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/calcein acetoxymethylester calcein am/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    calcein acetoxymethylester calcein am - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

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