calcein acetoxymethylester (Millipore)

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Name:

Calcein AM

Description:

Liposomes contain a self quenching fluorescent dye called calcein It is a fluorescent dye with excitation and emission wavelengths of 495 515 nm respectively It self quenches at concentrations above 70mM and is commonly used as an indicator of lipid vesicle leakage The leakage rate is calculated on the basis of the fluorescence increment as the entrapped calcein is leaked out of the liposomal compartment It is used as a complexometric indicator for titration of calcium ions with EDTA and for fluorometric determination of calcium

Catalog Number:

17783

Price:

None

Applications:

Non-fluorescent cell permeable derivative of calcein, becomes fluorescent on hydrolysis. Used as a neutral substrate for multidrug resistance protein, MRP; employed in tumor research.

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Structured Review

Millipore calcein acetoxymethylester
Calcein AM

calcein acetoxymethylester - by Bioz Stars, 2021-01

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Images

Microscopy:

Article Title: Antiangiogenic effects of decorin restored by unfractionated, low molecular weight, and nonanticoagulant heparins
Article Snippet: .. The media was then removed, stained with calcein-AM (Millipore), and visualized under the Olympus BX53 fluorescence microscope (Olympus, Tokyo, Japan). ..

Fluorescence:

Article Title: Mutations resulting in the formation of hyperactive complement convertases support cytocidal effect of anti-CD20 immunotherapeutics
Article Snippet: .. Fluorescence readout obtained for cells loaded with calcein-AM and lysed with 2% NP40 (Sigma) was considered as full lysis. .. Assays measuring complement consumption/complement activity restoration The concept of complement consumption assay was similar to that originally described by Beurskens et al. [ ].

Labeling:

Article Title: Membrane-Bound IL-21 Promotes Sustained Ex Vivo Proliferation of Human Natural Killer Cells
Article Snippet: .. Target cells were labeled with 0.5–5 µg/mL (titrated for each target cell line) of calcein-AM (Sigma-Aldrich) for 1 h at 37 °C with occasional shaking. .. Cells were co-cultured at the indicated effector-to-target (E∶T) ratios and incubated at 37 °C for 4 h. After incubation, 100 µL of the supernatant was harvested and transferred to a new plate.

Concentration Assay:

Article Title: Human Immunodeficiency Virus Protease Inhibitors Serve as Substrates for Multidrug Transporter Proteins MDR1 and MRP1 but Retain Antiviral Efficacy in Cell Lines Expressing These Transporters
Article Snippet: .. After a 2-min incubation, calcein-AM was added to a final concentration of 1 μM and the plates were placed in a cytofluorimeter (Millipore, Bedford, Mass.). .. Fluorescence was measured at regular intervals for 30 to 60 min with 485-nm (bandwidth, 20 nm) excitation and 530-nm (bandwidth, 25 nm) emission filters.

Incubation:

Article Title: Proteome Analysis Identified the PPAR? Ligand 15d-PGJ2 as a Novel Drug Inhibiting Melanoma Progression and Interfering with Tumor-Stroma Interaction
Article Snippet: .. For Calcein staining 12 or 24 h after seeding, cells were washed once with PBS and cells were incubated for 30 minutes at 37°C with 50 µL PBS containing 0.05% Calcein-AM (Sigma Aldrich, Vienna, Austria). .. Micrographs of fluorescent cells were taken using a Nikon Digital Sight DS-Fi1C CCD camera.

Infection:

Article Title: Distinct efficacy of HIV-1 entry inhibitors to prevent cell-to-cell transfer of R5 and X4 viruses across a human placental trophoblast barrier in a reconstitution model in vitro
Article Snippet: .. Human PBMCs, at day-7 post infection with HIV-1 LAI, were labelled with the green cytoplasmic dye calcein-AM (Sigma-Aldrich) at 1.5 μg/ml in PBS. ..

Lysis:

Staining:

Article Title: Surface modification of electrospun silk/AMOX/PVA nanofibers by dielectric barrier discharge plasma: physiochemical properties, drug delivery and in-vitro biocompatibility
Article Snippet: .. Live/dead staining The viable and dead cells seeded on different electrospun nanofibers were visualized using calcein-AM and ethidium homodimer (Sigma-Aldrich) staining after 48 h of culture. .. Briefly, the cell seeded nanofibers were washed with PBS once and incubated at 37 °C and 5% CO2 with 40 nM calcein-AM (stain live cells) and 20 nM ethidium homodimer (stain dead cells) for 20 min. Then, the dye was removed and washed the nanofibers twice with PBS, subsequently visualized underhydrated conditions in fluorescent microscope (EVOS XL digital microscope, Thermo Fisher Scientific, USA) with viable cells appearing green and dead cells appearing red.

Article Title: Glancing-Angle Deposition of Nanostructures on an Implant Material Surface
Article Snippet: .. For quantitative analysis, cells have been stained with calcein-AM and subsequently a calculation of the cell-covered surface was performed [ ]. ..