calcein acetoxymethylester am  (Millipore)


Bioz Verified Symbol Millipore is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Name:
    Calcein
    Description:
    Calcein is useful in fitting soft and hybrid contact lenses along with its evaluation It has a molecular weight of 710kDa and is generally preferred over fluorescein Calcein is known to stain devitalized tissue It is not preferred for highly hydrated soft lenses
    Catalog Number:
    c0875
    Price:
    None
    Applications:
    Calcein has been used:. in whole cell staining . in histomorphometry and micro-computed tomography. to label active bone-forming surfaces
    Buy from Supplier


    Structured Review

    Millipore calcein acetoxymethylester am
    Calcein
    Calcein is useful in fitting soft and hybrid contact lenses along with its evaluation It has a molecular weight of 710kDa and is generally preferred over fluorescein Calcein is known to stain devitalized tissue It is not preferred for highly hydrated soft lenses
    https://www.bioz.com/result/calcein acetoxymethylester am/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    calcein acetoxymethylester am - by Bioz Stars, 2020-09
    99/100 stars

    Images

    Related Articles

    Staining:

    Article Title: Polyaniline cryogels: Biocompatibility of novel conducting macroporous material
    Article Snippet: .. Differentiating cells were stained by calcein AM (10 μM) or fixed by 2% formaldehyde, and F-actin was stained by phaloidin-FITC (Sigma-Aldrich). .. Nuclei were counterstained by DAPI (Sigma-Aldrich, 10 ng mL−1 ).

    Article Title: From the Cover: Embryonic Exposure to TCDD Impacts Osteogenesis of the Axial Skeleton in Japanese medaka, Oryzias latipes
    Article Snippet: .. Approximately 12 h prior to imaging, DMSO- and TCDD-treated 20 dpf col10a1: nlGFP and twist: EGFP medaka were counterstained with 0.1% alizarin-3-methylimino-diacetic acid (alizarin complexone or ALC) (Sigma Aldrich, St Louis, Missouri, USA) in ERM for 2 h. Similarly, osx/sp7:mCherry medaka were counter-stained with 0.01% Calcein (Sigma Aldrich, St Louis, Missouri, USA) in ERM (pH 9.0) for 2 h. For both staining procedures, larvae were briefly washed twice with 1× ERM, and remained in 1× ERM overnight to allow equilibration of ALC and Calcein stains. .. A representative sample ( n =4–6) of stained individuals from each treatment were anesthetized in 0.03% (w/v) tricaine in 1× ERM, immobilized in 35 mm glass-bottom petri dishes (MatTek Corporation, Ashland, Massachusetts, USA) in 1.3% low-melting agarose (Apex BioResearch Products, Genesee Scientific, San Diego, California, USA) containing 0.03% tricaine/ERM.

    Injection:

    Article Title: Osteocytic oxygen sensing controls bone mass through epigenetic regulation of sclerostin
    Article Snippet: .. To analyse dynamic bone parameters, calcein (16 mg/kg body weight; Sigma-Aldrich) was administered via intraperitoneal injection 4 days and 1 day prior to sacrifice. .. The mineralizing surface (MS), mineral apposition rate (MAR) and bone formation rate (BFR) were quantified on unstained MMA-sections.

    Article Title: Blockade of Receptor-Activated Gi Signaling in Osteoblasts In Vivo Leads to Site-Specific Increases in Cortical and Cancellous Bone Formation
    Article Snippet: .. Dynamic histomorphometry Mice were injected with 20 mg/kg of calcein (Sigma-Aldrich, St Louis, MO, USA) 21 and 7 days before euthanasia and with 15 mg/kg of demeclocycline (Sigma-Aldrich) 2 days before euthanasia. .. Mice were euthanized at 8 weeks, and femurs were isolated, fixed in 10% NBF, and stored in 70% ethanol.

    Imaging:

    Article Title: From the Cover: Embryonic Exposure to TCDD Impacts Osteogenesis of the Axial Skeleton in Japanese medaka, Oryzias latipes
    Article Snippet: .. Approximately 12 h prior to imaging, DMSO- and TCDD-treated 20 dpf col10a1: nlGFP and twist: EGFP medaka were counterstained with 0.1% alizarin-3-methylimino-diacetic acid (alizarin complexone or ALC) (Sigma Aldrich, St Louis, Missouri, USA) in ERM for 2 h. Similarly, osx/sp7:mCherry medaka were counter-stained with 0.01% Calcein (Sigma Aldrich, St Louis, Missouri, USA) in ERM (pH 9.0) for 2 h. For both staining procedures, larvae were briefly washed twice with 1× ERM, and remained in 1× ERM overnight to allow equilibration of ALC and Calcein stains. .. A representative sample ( n =4–6) of stained individuals from each treatment were anesthetized in 0.03% (w/v) tricaine in 1× ERM, immobilized in 35 mm glass-bottom petri dishes (MatTek Corporation, Ashland, Massachusetts, USA) in 1.3% low-melting agarose (Apex BioResearch Products, Genesee Scientific, San Diego, California, USA) containing 0.03% tricaine/ERM.

    Mouse Assay:

    Article Title: Blockade of Receptor-Activated Gi Signaling in Osteoblasts In Vivo Leads to Site-Specific Increases in Cortical and Cancellous Bone Formation
    Article Snippet: .. Dynamic histomorphometry Mice were injected with 20 mg/kg of calcein (Sigma-Aldrich, St Louis, MO, USA) 21 and 7 days before euthanasia and with 15 mg/kg of demeclocycline (Sigma-Aldrich) 2 days before euthanasia. .. Mice were euthanized at 8 weeks, and femurs were isolated, fixed in 10% NBF, and stored in 70% ethanol.

    Article Title: Induction of periosteal bone formation by intraosseous BMP-2 injection in a mouse model of osteogenesis imperfecta
    Article Snippet: .. Mice were subcutaneously dosed with 10 mg/kg calcein (Sigma, NSW, Australia) at days –10 and –3 before cull. .. Mice were culled two weeks after surgery using a CO2 chamber.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Millipore calcein acetoxymethylester
    Calcein Acetoxymethylester, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/calcein acetoxymethylester/product/Millipore
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    calcein acetoxymethylester - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    99
    Millipore calcein acetoxymethylester calcein am
    Calcein Acetoxymethylester Calcein Am, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/calcein acetoxymethylester calcein am/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    calcein acetoxymethylester calcein am - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    Image Search Results