cacl2 h2 o  (Millipore)


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    Structured Review

    Millipore cacl2 h2 o
    Cacl2 H2 O, supplied by Millipore, used in various techniques. Bioz Stars score: 83/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cacl2 h2 o/product/Millipore
    Average 83 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    cacl2 h2 o - by Bioz Stars, 2020-02
    83/100 stars

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    Related Articles

    Isolation:

    Article Title: Loss of DPP4 activity is related to a prothrombogenic status of endothelial cells: implications for the coronary microvasculature of myocardial infarction patients
    Article Snippet: Cell culture Freshly isolated HUVECs were cultured in M199 culture medium containing heat-inactivated human serum (10% v/v), fetal calf serum (10% v/v), 2,500 Units heparin and 100 μg/ml endothelial cell growth factor (cells cultured in this medium served as controls). .. For metabolic inhibition, cells were incubated in 0.9 mM CaCl2 ·H2 O, 20 mM 2-deoxy-d -glucose (Sigma, St. Louis, MO, USA), 106 mM NaCl, 5 mM NaCN, 3.8 mM NaHCO3 , 4.4 mM KCl and 1 mM MgCl2 ·H2 O in H2 O (pH 6.6).

    Embryo Culture:

    Article Title: Changes in the transcriptome of morula-stage bovine embryos caused by heat shock: relationship to developmental acquisition of thermotolerance
    Article Snippet: Materials The fertilization medium SOF-FERT was made by modifying a custom formulation of Synthetic Oviduct Fluid manufactured by Specialty Media (Millipore, Billerica, MA, USA) to create the following formulation: 107.7 mM NaCl, 7.16 mM KCl, 1.19 mM KH2 PO4 , 1.17 mM CaCl2 ·H2 O, 5.3 mM sodium lactate, 0.20 mM sodium pyruvate, 25.07 mM NaHCO3 , 0.49 mM MgCl2 ·6H2 O, 6 mg/ml essentially fatty acid free BSA (Sigma, St. Louis, MO, USA), 5 μg/ml gentamicin, 10 μg/ml heparin, and 1.0 mM caffeine. .. The embryo culture medium was SOF-BE1 and was prepared as described elsewhere [ ].

    Cell Culture:

    Article Title: Loss of DPP4 activity is related to a prothrombogenic status of endothelial cells: implications for the coronary microvasculature of myocardial infarction patients
    Article Snippet: Paragraph title: Cell culture ... For metabolic inhibition, cells were incubated in 0.9 mM CaCl2 ·H2 O, 20 mM 2-deoxy-d -glucose (Sigma, St. Louis, MO, USA), 106 mM NaCl, 5 mM NaCN, 3.8 mM NaHCO3 , 4.4 mM KCl and 1 mM MgCl2 ·H2 O in H2 O (pH 6.6).

    Incubation:

    Article Title: Loss of DPP4 activity is related to a prothrombogenic status of endothelial cells: implications for the coronary microvasculature of myocardial infarction patients
    Article Snippet: .. For metabolic inhibition, cells were incubated in 0.9 mM CaCl2 ·H2 O, 20 mM 2-deoxy-d -glucose (Sigma, St. Louis, MO, USA), 106 mM NaCl, 5 mM NaCN, 3.8 mM NaHCO3 , 4.4 mM KCl and 1 mM MgCl2 ·H2 O in H2 O (pH 6.6). .. Apocynin (100 μM), diprotin A (10 mM), polymyxin B sulfate (2 μg/ml) and TNFα (10 μg/ml) (all Sigma) were incubated for 6 h.

    Article Title: Noninvasive, Quantitative, Spatiotemporal Characterization of Mineralization in Three-Dimensional Collagen Hydrogels Using High-Resolution Spectral Ultrasound Imaging
    Article Snippet: The standard (1×) mineralizing solution has final ion concentrations of 141 mM NaCl, 4.0 mM KCl, 2.5 mM CaCl2 ·H2 O, 1.0 mM MgCl2 , 4.2 mM NaHCO3 , 0.5 mM MgSO4 , and 1.0 mM KH2 PO4 (Sigma Aldrich). .. Collagen hydrogels were fabricated as described previously and were incubated at 37°C in 3.0 mL of either phosphate-buffered saline (PBS), 2.5×SBF, or 5×SBF.

    Inhibition:

    Article Title: Loss of DPP4 activity is related to a prothrombogenic status of endothelial cells: implications for the coronary microvasculature of myocardial infarction patients
    Article Snippet: .. For metabolic inhibition, cells were incubated in 0.9 mM CaCl2 ·H2 O, 20 mM 2-deoxy-d -glucose (Sigma, St. Louis, MO, USA), 106 mM NaCl, 5 mM NaCN, 3.8 mM NaHCO3 , 4.4 mM KCl and 1 mM MgCl2 ·H2 O in H2 O (pH 6.6). .. Apocynin (100 μM), diprotin A (10 mM), polymyxin B sulfate (2 μg/ml) and TNFα (10 μg/ml) (all Sigma) were incubated for 6 h.

    Activity Assay:

    Article Title: Direct Effects of Microalgae and Protists on Herring (Clupea harengus) Yolk Sac Larvae
    Article Snippet: To measure trypsin activity, individual larvae were slowly thawed on ice, dipped in de-ionized distilled water and their SL measured. .. Next, the tissue was homogenized with a micro pestle in Tris-buffer (0.1M) with CaCl2 *H2 O (0.02 M) (Sigma-Aldrich, Hamburg, Germany).

    Modification:

    Article Title: Direct Effects of Microalgae and Protists on Herring (Clupea harengus) Yolk Sac Larvae
    Article Snippet: Next, the tissue was homogenized with a micro pestle in Tris-buffer (0.1M) with CaCl2 *H2 O (0.02 M) (Sigma-Aldrich, Hamburg, Germany). .. Trypsin activity was measured spectrofluorometrically (Safas Monaco, Xenius, Monaco) in 96-well microplates (Nunc plates, VWR, Darmstadt, Germany) with Nα-benzoyl-L-arginin-4-methylcoumarinyl-7-amid as the substrate and 7-amino-4-methylcoumarin (both Bachem AG, Bubendorf, Swiss) as the fluorophore following a modified protocol from Ueberschär [ ].

    Fluorescence In Situ Hybridization:

    Article Title: Direct Effects of Microalgae and Protists on Herring (Clupea harengus) Yolk Sac Larvae
    Article Snippet: It is the most abundant proteolytic enzyme in marine fish larvae [ ] which is present directly after hatching and has been used to monitoring short-term variability in feeding [ ]. .. Next, the tissue was homogenized with a micro pestle in Tris-buffer (0.1M) with CaCl2 *H2 O (0.02 M) (Sigma-Aldrich, Hamburg, Germany).

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    Millipore cacl2 2 h2 o
    Cacl2 2 H2 O, supplied by Millipore, used in various techniques. Bioz Stars score: 96/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cacl2 2 h2 o/product/Millipore
    Average 96 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    cacl2 2 h2 o - by Bioz Stars, 2020-02
    96/100 stars
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