cacl2 2h2o  (Millipore)


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  • 94

    Structured Review

    Millipore cacl2 2h2o
    Cacl2 2h2o, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cacl2 2h2o/product/Millipore
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cacl2 2h2o - by Bioz Stars, 2020-02
    94/100 stars

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    Related Articles

    In Vitro:

    Article Title: Sustained Delivery of VEGF Maintains Innervation and Promotes Reperfusion in Ischemic Skeletal Muscles Via NGF/GDNF Signaling
    Article Snippet: Alginates were mixed with the 125 I-VEGF165 as described above to quantify VEGF release in vitro . .. The gels were divided into four samples and subsequently incubated in 3 ml of PBS, pH 7.2 (Invitrogen, Carlsbad, CA) with 0.1 g/l of MgCl2 .6H2O and 0.132 g/l of CaCl2 .2H2O (Sigma-Aldrich) in a 37 °C humidified incubator.

    Isolation:

    Article Title: Excitatory and inhibitory synaptic mechanisms at the first stage of integration in the electroreception system of the shark
    Article Snippet: Briefly, the brain stem and the afferent nerve were isolated and incubated in the experimental chamber with continuously superfusion of aerated, 20°C Ringer solution. .. The shark Ringer solution (modified from Hentschel et al., ), contained (in mM) 280 NaCl, 6 KCl, 5 CaCl2-2H2O, 3 MgCL2-6H2O, 0.5 Na2SO4, 1 NaH2PO4-12H2O, 8 NaHCO3, 350 urea, 72 trimethylamine N-oxide dehydrate (TMAO, Sigma, Rehovot, Israel), 5 glucose, 0.75 polyvinylpyrrolidone (PVP-40T, Sigma).

    Activation Assay:

    Article Title: Nociceptive interneurons control modular motor pathways to promote escape behavior in Drosophila
    Article Snippet: .. For activation of presynaptic neurons (Down and Back) with CsChrimson and imaging in Goro neurons, the central nervous system of wandering third instar larvae was dissected in cold physiological saline, Baines solution ( ) containing (in mM) 103 NaCl, 5 KCl, 5 HEPES, 26 NaHCO3 , 1 NaH2PO4 , 5 Trehalose, 6 Sucrose, 2 CaCl2 2H2O, 8 MgCl2 6H2 O, and kept stable by sticking them on poly-L-lysine (SIGMA, P1524) coated cover glass placed in small Sylgard (Dow Corning) plates. .. 620 nm LED (Mightex Systems Inc.) was used for whole CNS CsChrimson activation and 1040 nm laser using Phaser module (Intelligent Imaging Innovations, Inc.) for localized CsChrimson activation.

    Incubation:

    Article Title: Protease signaling through protease activated receptor 1 mediate nerve activation by mucosal supernatants from irritable bowel syndrome but not from ulcerative colitis patients
    Article Snippet: During the endoscopy, 4 biopsies were collected from the descending colon and rapidly immersed in either 1 ml carbogen-aerated (95% O2/5% CO2) 37°C warm Hank’s solution (n = 13), (Sigma, St. Louis, MO) or in 1 ml oxygenated (100% O2 or ambient air) 37°C warm HEPES/Krebs buffer (pH 7.4) containing in mM: 135 NaCl, 5.4 KCl, 1 MgCl2·6H2O, 1.2 NaH2PO4, 3 HEPES, 1.25 CaCl2·2H2O and 12.5 glucose (all from Sigma, Steinheim, Germany) (n = 26). .. After an incubation of 25 min, the supernatants were centrifuged at 200–4000g (for HEPES/Krebs supernatants Spin-X micro tubes (Corning Life Science) were used), aliquoted and stored at -70°C until use.

    Article Title: Sustained Delivery of VEGF Maintains Innervation and Promotes Reperfusion in Ischemic Skeletal Muscles Via NGF/GDNF Signaling
    Article Snippet: .. The gels were divided into four samples and subsequently incubated in 3 ml of PBS, pH 7.2 (Invitrogen, Carlsbad, CA) with 0.1 g/l of MgCl2 .6H2O and 0.132 g/l of CaCl2 .2H2O (Sigma-Aldrich) in a 37 °C humidified incubator. .. At each experimental time point, the radiolabeled growth factor present in the buffer solution was measured using a gamma counter (1470 WIZARD; PerkinElmer).

    Article Title: Excitatory and inhibitory synaptic mechanisms at the first stage of integration in the electroreception system of the shark
    Article Snippet: Briefly, the brain stem and the afferent nerve were isolated and incubated in the experimental chamber with continuously superfusion of aerated, 20°C Ringer solution. .. The shark Ringer solution (modified from Hentschel et al., ), contained (in mM) 280 NaCl, 6 KCl, 5 CaCl2-2H2O, 3 MgCL2-6H2O, 0.5 Na2SO4, 1 NaH2PO4-12H2O, 8 NaHCO3, 350 urea, 72 trimethylamine N-oxide dehydrate (TMAO, Sigma, Rehovot, Israel), 5 glucose, 0.75 polyvinylpyrrolidone (PVP-40T, Sigma).

    Imaging:

    Article Title: Nociceptive interneurons control modular motor pathways to promote escape behavior in Drosophila
    Article Snippet: .. For activation of presynaptic neurons (Down and Back) with CsChrimson and imaging in Goro neurons, the central nervous system of wandering third instar larvae was dissected in cold physiological saline, Baines solution ( ) containing (in mM) 103 NaCl, 5 KCl, 5 HEPES, 26 NaHCO3 , 1 NaH2PO4 , 5 Trehalose, 6 Sucrose, 2 CaCl2 2H2O, 8 MgCl2 6H2 O, and kept stable by sticking them on poly-L-lysine (SIGMA, P1524) coated cover glass placed in small Sylgard (Dow Corning) plates. .. 620 nm LED (Mightex Systems Inc.) was used for whole CNS CsChrimson activation and 1040 nm laser using Phaser module (Intelligent Imaging Innovations, Inc.) for localized CsChrimson activation.

    Modification:

    Article Title: Excitatory and inhibitory synaptic mechanisms at the first stage of integration in the electroreception system of the shark
    Article Snippet: .. The shark Ringer solution (modified from Hentschel et al., ), contained (in mM) 280 NaCl, 6 KCl, 5 CaCl2-2H2O, 3 MgCL2-6H2O, 0.5 Na2SO4, 1 NaH2PO4-12H2O, 8 NaHCO3, 350 urea, 72 trimethylamine N-oxide dehydrate (TMAO, Sigma, Rehovot, Israel), 5 glucose, 0.75 polyvinylpyrrolidone (PVP-40T, Sigma). ..

    MANN-WHITNEY:

    Article Title: Protease signaling through protease activated receptor 1 mediate nerve activation by mucosal supernatants from irritable bowel syndrome but not from ulcerative colitis patients
    Article Snippet: During the endoscopy, 4 biopsies were collected from the descending colon and rapidly immersed in either 1 ml carbogen-aerated (95% O2/5% CO2) 37°C warm Hank’s solution (n = 13), (Sigma, St. Louis, MO) or in 1 ml oxygenated (100% O2 or ambient air) 37°C warm HEPES/Krebs buffer (pH 7.4) containing in mM: 135 NaCl, 5.4 KCl, 1 MgCl2·6H2O, 1.2 NaH2PO4, 3 HEPES, 1.25 CaCl2·2H2O and 12.5 glucose (all from Sigma, Steinheim, Germany) (n = 26). .. The neuroindex evoked by IBS supernatants incubated in Hank´s (n = 8, 270 (218./429)) or in HEPES/Krebs buffer (n = 6, 240 (164/372) did not differ ( P = 0.573, Mann-Whitney Rank Sum Test).

    Software:

    Article Title: Nociceptive interneurons control modular motor pathways to promote escape behavior in Drosophila
    Article Snippet: For activation of presynaptic neurons (Down and Back) with CsChrimson and imaging in Goro neurons, the central nervous system of wandering third instar larvae was dissected in cold physiological saline, Baines solution ( ) containing (in mM) 103 NaCl, 5 KCl, 5 HEPES, 26 NaHCO3 , 1 NaH2PO4 , 5 Trehalose, 6 Sucrose, 2 CaCl2 2H2O, 8 MgCl2 6H2 O, and kept stable by sticking them on poly-L-lysine (SIGMA, P1524) coated cover glass placed in small Sylgard (Dow Corning) plates. .. Image data were processed by ImageJ software (NIH) and analyzed using custom code written in MATLAB (Mathworks).