Structured Review

Merck KGaA cacl2 2h2 o
Cacl2 2h2 O, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cacl2 2h2 o/product/Merck KGaA
Average 99 stars, based on 2 article reviews
Price from $9.99 to $1999.99
cacl2 2h2 o - by Bioz Stars, 2020-02
99/100 stars

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Amplification:

Article Title: Capacity of Nine Thermostable DNA Polymerases To Mediate DNA Amplification in the Presence of PCR-Inhibiting Samples
Article Snippet: .. Different concentrations of CaCl2 · 2H2 O, KCl, MgCl2 · 6H2 O, and NaCl (Merck, Darmstadt, Germany) were prepared, as sterile solutions, to study their effect on the amplification capacity of the nine polymerases. ..

In Vitro:

Article Title: A Predominance of Clade 17 Candida albicans Isolated From Hemocultures in a Tertiary Care Hospital in Thailand
Article Snippet: Paragraph title: In vitro Virulence Study ... One hundred milliliters of medium containing 13.0 g SDA, 11.7 g NaCl (Merck, Germany) SDA, 11.7 g NaCl (Merck, Germany), 1.48 g CaCl2 .2H2 O (Merck, Germany), 2% agar (Oxoid, United Kingdom), and 10% sterile egg yolk (Merck, Germany) was prepared to determine extracellular phospholipase activity ( ).

Modification:

Article Title: A Predominance of Clade 17 Candida albicans Isolated From Hemocultures in a Tertiary Care Hospital in Thailand
Article Snippet: One hundred milliliters of medium containing 13.0 g SDA, 11.7 g NaCl (Merck, Germany) SDA, 11.7 g NaCl (Merck, Germany), 1.48 g CaCl2 .2H2 O (Merck, Germany), 2% agar (Oxoid, United Kingdom), and 10% sterile egg yolk (Merck, Germany) was prepared to determine extracellular phospholipase activity ( ). .. Candida albicans strains’ proteinase activity was analyzed on bovine serum albumin medium by the modified Staib method ( ; ).

Article Title: The 3D Printing of Calcium Phosphate with K-Carrageenan under Conditions Permitting the Incorporation of Biological Components—A Method
Article Snippet: Production of Calcium Phosphate Paste The CaP paste is produced by a precipitation reaction according to a modified version of a coating protocol described earlier [ , ]. .. In brief, a supersaturated CaP solution containing 684 mM NaCl (31434-1KG-M, Sigma-Aldrich, St Louis, MO, USA), 20 mM CaCl2 ·2H2 O (102382, Merck Millipore, Darmstadt, Germany), 10.1 mM Na2 HPO4 ·2H2 O (106580, Merck Millipore, Darmstadt, Germany), and 200 mM HCl (100317, Merck Millipore, Darmstadt, Germany) is made.

Concentration Assay:

Article Title: The Buffer Capacity and Calcium Concentration of Water Influence the Microbial Species Diversity, Grain Growth, and Metabolite Production During Water Kefir Fermentation
Article Snippet: .. This water kefir grain mass was used as inoculum to start eight series of water kefir fermentations , which differed in (i) the buffer capacity, indicated with the letter B preceded by a number representing the concentration of HCO3 – (added as KHCO3 ; Sigma–Aldrich, Saint Louis, MO, United States) relative to a basic concentration of 313 mg l–1 (=1), which corresponds with the buffer capacity of untreated tap water (Brussels, Belgium) (indications 0B, 1B, and 2B) or (ii) the calcium concentration of the water used for fermentation, indicated with the letters Ca preceded by a number representing the concentration of Ca2+ [added as CaCl2 .2H2 O (Merck, Darmstadt, Germany)] relative to a basic concentration of 50 mg l–1 , which corresponds with an average calcium concentration in drinking water ( ) (indications 0Ca, 1Ca, and 4Ca). .. Hereto, ultrapure water (18.2 MΩ⋅cm at 25°C) was used, obtained from a gradient A10 Milli-Q water purification system (EMD Millipore, Billerica, MA, United States), that was supplemented with either KHCO3 (0, 313, or 626 mg l–1 ) or CaCl2 .2H2 O (0, 50, or 200 mg l–1 ), corresponding with the concentrations of the fermentations mentioned above.

Protease Inhibitor:

Article Title: The GPCR-associated sorting protein 1 regulates ligand-induced down-regulation of GPR55
Article Snippet: Complete Protease inhibitor cocktail tablets were from Roche (Vienna, Austria) and PNGaseF from New England Biolabs (Frankfurt, Germany). .. NaOH, KCl and CaCl2 *2H2 O were obtained from Merck (Vienna, Austria), NaCl, Tris and formaldehyde were from Roth (Lactan, Austria).

Reporter Gene Assay:

Article Title: Heteromerization of human cytomegalovirus encoded chemokine receptors
Article Snippet: The white 96-well culture plates and the Steadylite Plus Assay Kit, high sensitivity luminescence reporter gene assay system, [3 H]myo-inositol and EasyTag 35 S-methionine were purchased from PerkinElmer (Austria). .. NaOH, KCl and CaCl2 ·2H2 O were obtained from Merck (Austria), NaCl, Tris and formaldehyde were from Roth (Lactan, Austria).

Cell Culture:

Article Title: Heteromerization of human cytomegalovirus encoded chemokine receptors
Article Snippet: Cell culture media (DMEM), fetal bovine serum (FBS), Dulbecco's phosphate buffered saline (DPBS), Lipofectamine 2000, Alexa Fluor 594 nm conjugated IgG1a, 488 nm conjugated IgG2b and 594 nm conjugated donkey anti rabbit IgG were purchased from Invitrogen (Austria). .. NaOH, KCl and CaCl2 ·2H2 O were obtained from Merck (Austria), NaCl, Tris and formaldehyde were from Roth (Lactan, Austria).

Purification:

Article Title: The Buffer Capacity and Calcium Concentration of Water Influence the Microbial Species Diversity, Grain Growth, and Metabolite Production During Water Kefir Fermentation
Article Snippet: This water kefir grain mass was used as inoculum to start eight series of water kefir fermentations , which differed in (i) the buffer capacity, indicated with the letter B preceded by a number representing the concentration of HCO3 – (added as KHCO3 ; Sigma–Aldrich, Saint Louis, MO, United States) relative to a basic concentration of 313 mg l–1 (=1), which corresponds with the buffer capacity of untreated tap water (Brussels, Belgium) (indications 0B, 1B, and 2B) or (ii) the calcium concentration of the water used for fermentation, indicated with the letters Ca preceded by a number representing the concentration of Ca2+ [added as CaCl2 .2H2 O (Merck, Darmstadt, Germany)] relative to a basic concentration of 50 mg l–1 , which corresponds with an average calcium concentration in drinking water ( ) (indications 0Ca, 1Ca, and 4Ca). .. Hereto, ultrapure water (18.2 MΩ⋅cm at 25°C) was used, obtained from a gradient A10 Milli-Q water purification system (EMD Millipore, Billerica, MA, United States), that was supplemented with either KHCO3 (0, 313, or 626 mg l–1 ) or CaCl2 .2H2 O (0, 50, or 200 mg l–1 ), corresponding with the concentrations of the fermentations mentioned above.

Produced:

Article Title: The 3D Printing of Calcium Phosphate with K-Carrageenan under Conditions Permitting the Incorporation of Biological Components—A Method
Article Snippet: Production of Calcium Phosphate Paste The CaP paste is produced by a precipitation reaction according to a modified version of a coating protocol described earlier [ , ]. .. In brief, a supersaturated CaP solution containing 684 mM NaCl (31434-1KG-M, Sigma-Aldrich, St Louis, MO, USA), 20 mM CaCl2 ·2H2 O (102382, Merck Millipore, Darmstadt, Germany), 10.1 mM Na2 HPO4 ·2H2 O (106580, Merck Millipore, Darmstadt, Germany), and 200 mM HCl (100317, Merck Millipore, Darmstadt, Germany) is made.

Sequencing:

Article Title: Microfluidic chip system for the selection and enrichment of cell binding aptamers
Article Snippet: SB contains oligonucleotides with a randomized sequence region of 49 nucleotides (N) in the center, which is flanked by fixed primer hybridization regions: 5′-FAM-[spacer-C-18]-GCCTGTTGTGAGCCTCCTAAC-N49-CATGCTTATTCTTGTCTCCC-3′. .. To perform the microfluidic chip experiments, a dielectrophoresis buffer with a low conductivity (200 μ S/cm) containing 3 mM sucrose (Sigma-Aldrich, St. Louis, USA), 0.901 mM CaCl2 ·2H2 O (Merck, Darmstadt, Germany), and 0.493 mM MgCl2 (Merck Schuchardt, Hohenbrunn, Germany) was established.

Incubation:

Article Title: A Predominance of Clade 17 Candida albicans Isolated From Hemocultures in a Tertiary Care Hospital in Thailand
Article Snippet: One hundred milliliters of medium containing 13.0 g SDA, 11.7 g NaCl (Merck, Germany) SDA, 11.7 g NaCl (Merck, Germany), 1.48 g CaCl2 .2H2 O (Merck, Germany), 2% agar (Oxoid, United Kingdom), and 10% sterile egg yolk (Merck, Germany) was prepared to determine extracellular phospholipase activity ( ). .. Five microliters of a yeast suspension (108 cells/ml) of the tested and control strains was dropped onto the surface of the egg yolk–containing plate and incubated at 37°C for 48 h to measure a precipitation zone around the colony.

Article Title: The 3D Printing of Calcium Phosphate with K-Carrageenan under Conditions Permitting the Incorporation of Biological Components—A Method
Article Snippet: In brief, a supersaturated CaP solution containing 684 mM NaCl (31434-1KG-M, Sigma-Aldrich, St Louis, MO, USA), 20 mM CaCl2 ·2H2 O (102382, Merck Millipore, Darmstadt, Germany), 10.1 mM Na2 HPO4 ·2H2 O (106580, Merck Millipore, Darmstadt, Germany), and 200 mM HCl (100317, Merck Millipore, Darmstadt, Germany) is made. .. After adjusting the pH of the solution, incubation is performed overnight in a shaking water bath at 37 °C to allow CaP precipitation.

Article Title: Listeria monocytogenes Strains Underrepresented during Selective Enrichment with an ISO Method Might Dominate during Passage through Simulated Gastric Fluid and In Vitro Infection of Caco-2 Cells
Article Snippet: SGF was prepared according to the method described by Barmpalia-Davis et al. ( ) and consisted of the following reagents (per liter): 0.4 g glucose (Riedel de Haën, Switzerland), 3.0 g yeast extract (Lab M Limited, United Kingdom), 1.0 g Bacto peptone (Lab M Limited, United Kingdom), 4.0 g porcine mucin (Sigma-Aldrich Co., USA), 0.5 g cysteine (Sigma-Aldrich Co., USA), 0.08 g NaCl (Merck KGaA, Germany), 0.4 g NaHCO3 (PanReac AppliChem, Spain), 0.04 g K2 HPO4 (Merck KGaA, Germany), 0.008 g CaCl2 -2H2 O (Merck KGaA, Germany), 0.008 g MgSO4 ·7H2 O (Mallinckrodt Pharmaceuticals, Ireland), 1.0 g xylan (Sigma-Aldrich, Co., USA), 3.0 g soluble starch (Merck KGaA, Germany), 2.0 g pectin (Sigma-Aldrich, Co., USA), and 1 ml Tween 80 (Scharlab S.L., Spain). .. The survival of L. monocytogenes strains in SGF was evaluated for single and mixed TSB-Y (10°C) cultures as follows: on days 2, 4, 6, and 8 of incubation, 2-ml volumes of the cultures were centrifuged (10,000 × g for 1 min), resuspended in 2 ml of SGF (37°C), and incubated in a water bath at 37°C for total exposure times of 18, 48, 60, and 90 min, respectively.

Selection:

Article Title: Microfluidic chip system for the selection and enrichment of cell binding aptamers
Article Snippet: A fluorescein-labeled primer with an 18-carbon ethylene glycol spacer (5′ FAM [spacer-C18]-GCCTGTTGTGAGCCTCCTAAC-3′) was used in the PCR to monitor the enrichment of cell binding oligonucleotides during the selection process. .. To perform the microfluidic chip experiments, a dielectrophoresis buffer with a low conductivity (200 μ S/cm) containing 3 mM sucrose (Sigma-Aldrich, St. Louis, USA), 0.901 mM CaCl2 ·2H2 O (Merck, Darmstadt, Germany), and 0.493 mM MgCl2 (Merck Schuchardt, Hohenbrunn, Germany) was established.

Activity Assay:

Article Title: A Predominance of Clade 17 Candida albicans Isolated From Hemocultures in a Tertiary Care Hospital in Thailand
Article Snippet: .. One hundred milliliters of medium containing 13.0 g SDA, 11.7 g NaCl (Merck, Germany) SDA, 11.7 g NaCl (Merck, Germany), 1.48 g CaCl2 .2H2 O (Merck, Germany), 2% agar (Oxoid, United Kingdom), and 10% sterile egg yolk (Merck, Germany) was prepared to determine extracellular phospholipase activity ( ). .. Five microliters of a yeast suspension (108 cells/ml) of the tested and control strains was dropped onto the surface of the egg yolk–containing plate and incubated at 37°C for 48 h to measure a precipitation zone around the colony.

Polymerase Chain Reaction:

Article Title: Capacity of Nine Thermostable DNA Polymerases To Mediate DNA Amplification in the Presence of PCR-Inhibiting Samples
Article Snippet: Different concentrations of CaCl2 · 2H2 O, KCl, MgCl2 · 6H2 O, and NaCl (Merck, Darmstadt, Germany) were prepared, as sterile solutions, to study their effect on the amplification capacity of the nine polymerases. .. Five microliters of the different ion concentrations were added to the PCR mixtures of the nine polymerases containing 1 ng of L. monocytogenes DNA per reaction tube.

Article Title: Microfluidic chip system for the selection and enrichment of cell binding aptamers
Article Snippet: A fluorescein-labeled primer with an 18-carbon ethylene glycol spacer (5′ FAM [spacer-C18]-GCCTGTTGTGAGCCTCCTAAC-3′) was used in the PCR to monitor the enrichment of cell binding oligonucleotides during the selection process. .. To perform the microfluidic chip experiments, a dielectrophoresis buffer with a low conductivity (200 μ S/cm) containing 3 mM sucrose (Sigma-Aldrich, St. Louis, USA), 0.901 mM CaCl2 ·2H2 O (Merck, Darmstadt, Germany), and 0.493 mM MgCl2 (Merck Schuchardt, Hohenbrunn, Germany) was established.

Western Blot:

Article Title: The GPCR-associated sorting protein 1 regulates ligand-induced down-regulation of GPR55
Article Snippet: ECL Western Blotting Substrate was from Pierce (THP, Austria). .. NaOH, KCl and CaCl2 *2H2 O were obtained from Merck (Vienna, Austria), NaCl, Tris and formaldehyde were from Roth (Lactan, Austria).

Binding Assay:

Article Title: Microfluidic chip system for the selection and enrichment of cell binding aptamers
Article Snippet: A fluorescein-labeled primer with an 18-carbon ethylene glycol spacer (5′ FAM [spacer-C18]-GCCTGTTGTGAGCCTCCTAAC-3′) was used in the PCR to monitor the enrichment of cell binding oligonucleotides during the selection process. .. To perform the microfluidic chip experiments, a dielectrophoresis buffer with a low conductivity (200 μ S/cm) containing 3 mM sucrose (Sigma-Aldrich, St. Louis, USA), 0.901 mM CaCl2 ·2H2 O (Merck, Darmstadt, Germany), and 0.493 mM MgCl2 (Merck Schuchardt, Hohenbrunn, Germany) was established.

Chromatin Immunoprecipitation:

Article Title: Microfluidic chip system for the selection and enrichment of cell binding aptamers
Article Snippet: .. To perform the microfluidic chip experiments, a dielectrophoresis buffer with a low conductivity (200 μ S/cm) containing 3 mM sucrose (Sigma-Aldrich, St. Louis, USA), 0.901 mM CaCl2 ·2H2 O (Merck, Darmstadt, Germany), and 0.493 mM MgCl2 (Merck Schuchardt, Hohenbrunn, Germany) was established. ..

Hybridization:

Article Title: Microfluidic chip system for the selection and enrichment of cell binding aptamers
Article Snippet: SB contains oligonucleotides with a randomized sequence region of 49 nucleotides (N) in the center, which is flanked by fixed primer hybridization regions: 5′-FAM-[spacer-C-18]-GCCTGTTGTGAGCCTCCTAAC-N49-CATGCTTATTCTTGTCTCCC-3′. .. To perform the microfluidic chip experiments, a dielectrophoresis buffer with a low conductivity (200 μ S/cm) containing 3 mM sucrose (Sigma-Aldrich, St. Louis, USA), 0.901 mM CaCl2 ·2H2 O (Merck, Darmstadt, Germany), and 0.493 mM MgCl2 (Merck Schuchardt, Hohenbrunn, Germany) was established.

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    Merck KGaA calcium chloride dihydrate
    Calcium Chloride Dihydrate, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/calcium chloride dihydrate/product/Merck KGaA
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    calcium chloride dihydrate - by Bioz Stars, 2020-02
    98/100 stars
      Buy from Supplier

    99
    Merck KGaA cacl2 2h2 o
    Cacl2 2h2 O, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cacl2 2h2 o/product/Merck KGaA
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    cacl2 2h2 o - by Bioz Stars, 2020-02
    99/100 stars
      Buy from Supplier

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