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Ipsen Group ca2 influx
Ca2 Influx, supplied by Ipsen Group, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 86 stars, based on 1 article reviews
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ca2 influx - by Bioz Stars, 2021-06
86/100 stars

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Blocking Assay:

Article Title: Control of neuronal ion channel function by glycogen synthase kinase-3: new prospective for an old kinase
Article Snippet: All of these molecular events are tightly regulated by intracellular Ca2+ concentration ([Ca2+ ]i ) and by the phosphorylation of the synprint sites on N-type and P/Q-type Cav channels (Yokoyama et al., ). .. By phosphorylation of the synprint site, GSK-3 decreases Ca2+ currents and Ca2+ influx in hippocampal neurons (Zhu et al., ), preventing the channel's association with SNAP25, syntaxin (Kim and Catterall, ; Yokoyama et al., , ), and synaptotagmin blocking presynaptic vesicle release (Zhu et al., ). .. This effect is remarkably similar to previously reported Cdk5/p25-dependent phosphorylation of the same II and III (LII−III ) loop of P/Q-type Cav channels, which also resulted in inhibition of the interaction of the channel with the SNARE complex required for neurotransmitter release (Tomizawa et al., ).

Functional Assay:

Article Title: Molecular interactions of the plasma membrane calcium ATPase PMCA2 at pre- and post-synaptic sites in rat cerebellum
Article Snippet: However, we are confident that our results are not false positives for a number of reasons: (i) we carried out immunoprecipitations using magnetic beads rather than agarose or sepharose resins, (ii) if the syntaxin was binding to the beads directly we would have detected it in control immunoprecipitations using IgG in place of the precipitating antibody, and (iii) the reciprocal immunoprecipitation using anti syntaxin to co-precipitate PMCA would have yielded negative results had the syntaxin simply bound to the beads. .. At a functional level we could detect the PMCA2-syntaxin interaction in all regions of the cerebellar cortex characterized by a high density of synaptic terminals, although it was greatest in the granule cell layer. .. Syntaxin-1A binds to L-, N- and P/Q-type voltage-gated Ca2+ channels and influences the location and gating of these important channels; with obvious importance for the regulation of Ca2+ influx and subsequent neurotransmitter release (Sheng et al ., 1994; ; ).

Activity Assay:

Article Title: Genome-wide RNAi screen of Ca2+ influx identifies genes that regulate Ca2+ release-activated Ca2+ channel activity
Article Snippet: The gene products of both Syx5 and Syx1A are t-SNARE proteins involved in vesicle fusion in many cell types. .. We verified the RNAi effects of Syx5 at the single-cell level and demonstrated strong suppression of CRAC channel activity as well as the SOC influx. tsr may regulate SOC influx indirectly by controlling cell metabolism because RNAi of tsr did not significantly influence CRAC current density in whole-cell patch–clamp experiments. .. Membrane trafficking previously was suggested to be important for SOC channel activity in Xenopus oocytes, based on inhibition by botulinum toxin or by a dominant-negative SNAP-25 construct , and our results further suggest a requirement for syntaxins and SNARE-complex formation, possibly to mediate translocation of Stim to the plasma membrane ( , ).

Patch Clamp:

Article Title: Genome-wide RNAi screen of Ca2+ influx identifies genes that regulate Ca2+ release-activated Ca2+ channel activity
Article Snippet: The gene products of both Syx5 and Syx1A are t-SNARE proteins involved in vesicle fusion in many cell types. .. We verified the RNAi effects of Syx5 at the single-cell level and demonstrated strong suppression of CRAC channel activity as well as the SOC influx. tsr may regulate SOC influx indirectly by controlling cell metabolism because RNAi of tsr did not significantly influence CRAC current density in whole-cell patch–clamp experiments. .. Membrane trafficking previously was suggested to be important for SOC channel activity in Xenopus oocytes, based on inhibition by botulinum toxin or by a dominant-negative SNAP-25 construct , and our results further suggest a requirement for syntaxins and SNARE-complex formation, possibly to mediate translocation of Stim to the plasma membrane ( , ).

Purification:

Article Title: Stimulation of Ca2+ influx through ATP receptors on rat brain synaptosomes: identification of functional P2X7 receptor subtypes
Article Snippet: P2X7 receptor protein was identified by Western blotting and immunohistochemical staining. .. Purified preparations were devoid of significant concentrations of GFAP or the microglial marker OX-42 but contained greatly enriched amounts of syntaxin and SNAP 25. .. The various pharmacological and biochemical studies were all consistent with the presence of functional P2X7 receptors.

Marker:

Article Title: Stimulation of Ca2+ influx through ATP receptors on rat brain synaptosomes: identification of functional P2X7 receptor subtypes
Article Snippet: P2X7 receptor protein was identified by Western blotting and immunohistochemical staining. .. Purified preparations were devoid of significant concentrations of GFAP or the microglial marker OX-42 but contained greatly enriched amounts of syntaxin and SNAP 25. .. The various pharmacological and biochemical studies were all consistent with the presence of functional P2X7 receptors.

Expressing:

Article Title: Characterization of Stanniocalcin 2, a Novel Target of the Mammalian Unfolded Protein Response with Cytoprotective Properties
Article Snippet: Given the negative regulation of Ca2+ influx by fish STC and mammalian STC1 , it is very likely that STC2 also functions in a similar manner by regulating Ca2+ homeostasis in cells subject to ER stress, thus promoting cell survival. .. Finally, although STC2 expression is induced by treatment of cells with tunicamycin, siRNA and overexpression experiments showed no correlation between STC2 expression and cell viability in the presence of tunicamycin, suggesting that protein N glycosylation of STC2 or other protein(s) is critical for cytoprotective properties of STC2 during ER stress. .. The manner in which STC2 expression is induced indicates that STC2 function is specifically required during the cellular response to challenge with ER stress agents, oxidative stress, and hypoxia (Fig. ).

Over Expression:

Article Title: Characterization of Stanniocalcin 2, a Novel Target of the Mammalian Unfolded Protein Response with Cytoprotective Properties
Article Snippet: Given the negative regulation of Ca2+ influx by fish STC and mammalian STC1 , it is very likely that STC2 also functions in a similar manner by regulating Ca2+ homeostasis in cells subject to ER stress, thus promoting cell survival. .. Finally, although STC2 expression is induced by treatment of cells with tunicamycin, siRNA and overexpression experiments showed no correlation between STC2 expression and cell viability in the presence of tunicamycin, suggesting that protein N glycosylation of STC2 or other protein(s) is critical for cytoprotective properties of STC2 during ER stress. .. The manner in which STC2 expression is induced indicates that STC2 function is specifically required during the cellular response to challenge with ER stress agents, oxidative stress, and hypoxia (Fig. ).

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    Ipsen Group ca2 influx
    Ca2 Influx, supplied by Ipsen Group, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ca2 influx/product/Ipsen Group
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ca2 influx - by Bioz Stars, 2021-06
    86/100 stars
      Buy from Supplier

    86
    Ipsen Group synaptosomal ca2 influx
    Synaptosomal Ca2 Influx, supplied by Ipsen Group, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/synaptosomal ca2 influx/product/Ipsen Group
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    synaptosomal ca2 influx - by Bioz Stars, 2021-06
    86/100 stars
      Buy from Supplier

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