ca2 indicator fluo 4  (Thermo Fisher)


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    Name:
    Fluo 4 AM Packaged For High Throughput Screening
    Description:
    Labeled calcium indicators are molecules that exhibit an increase in fluorescence upon binding Ca2 Fluo 3 has been used to image the spatial dynamics of Ca2 signaling in flow cytometry experiments involving photoactivation of caged chelators second messengers and neurotransmitters and for cell based pharmacological screening Fluo 4 is an analog of fluo 3 with the two chlorine substituents replaced by fluorines which results in increased fluorescence excitation at 488 nm and consequently higher fluorescence signal levels Cells may be loaded with the AM ester forms of these calcium indicators by adding the dissolved indicator directly to dishes containing cultured cells These indicators are useful for fluorescence and confocal microscopy flow cytometry and microplate screening applications Calcium Indicator AM Ester Specifications • Label Ex Em of Ca2 bound form Fluo 4 494 506 nm • Fluorescence intensity increase upon binding Ca2 100 fold• Kd for Ca2 in buffer 335 nM• Exhibit fluorescence increase upon binding Ca2 with little shift in wavelengthUsing TPEN to Control Heavy Metal Cations In addition BAPTA based indicators such as these bind various heavy metal cations e g Mn2 Zn2 Pb2 with substantially higher affinity than Ca2 Perturbations to calcium measurements caused by presence of these ions can be controlled using the heavy metal selective chelator TPEN More Choices for Fluorescent Calcium Indicators We offer a large selection of Molecular Probes calcium indicators for use in various experimental scenarios For more information review Fluorescent Ca2 Indicators Excited with Visible Light Section 19 3 in the Molecular Probes Handbook For UV excitable Ca2 indicators protein based Ca2 indicators conjugates of Ca2 indicators and for fluorescence based indicators of other metal ions i e Mg2 Zn2 review Indicators for Ca2 Mg2 Zn2 and Other Metal Ions Chapter 19 in the Molecular Probes Handbook For Research Use Only Not for human or animal therapeutic or diagnostic use
    Catalog Number:
    f14202
    Price:
    None
    Applications:
    Calcium Detection|Calcium Indicator Assays|Cell Analysis|Cell-Based Ion Channel Assays|Cell-Based Reporter Assays|Cell-Based Second Messenger Assays|Cellular Imaging|G-Protein Coupled Receptor Biology|Industrial & Applied Science|Ion Channel Biology|Ionic Homeostasis & Signaling|Pharma & Biopharma|Target & Lead Identification & Validation|Cell Viability, Proliferation & Function
    Category:
    Labeling Detection Products
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    Structured Review

    Thermo Fisher ca2 indicator fluo 4
    Labeled calcium indicators are molecules that exhibit an increase in fluorescence upon binding Ca2 Fluo 3 has been used to image the spatial dynamics of Ca2 signaling in flow cytometry experiments involving photoactivation of caged chelators second messengers and neurotransmitters and for cell based pharmacological screening Fluo 4 is an analog of fluo 3 with the two chlorine substituents replaced by fluorines which results in increased fluorescence excitation at 488 nm and consequently higher fluorescence signal levels Cells may be loaded with the AM ester forms of these calcium indicators by adding the dissolved indicator directly to dishes containing cultured cells These indicators are useful for fluorescence and confocal microscopy flow cytometry and microplate screening applications Calcium Indicator AM Ester Specifications • Label Ex Em of Ca2 bound form Fluo 4 494 506 nm • Fluorescence intensity increase upon binding Ca2 100 fold• Kd for Ca2 in buffer 335 nM• Exhibit fluorescence increase upon binding Ca2 with little shift in wavelengthUsing TPEN to Control Heavy Metal Cations In addition BAPTA based indicators such as these bind various heavy metal cations e g Mn2 Zn2 Pb2 with substantially higher affinity than Ca2 Perturbations to calcium measurements caused by presence of these ions can be controlled using the heavy metal selective chelator TPEN More Choices for Fluorescent Calcium Indicators We offer a large selection of Molecular Probes calcium indicators for use in various experimental scenarios For more information review Fluorescent Ca2 Indicators Excited with Visible Light Section 19 3 in the Molecular Probes Handbook For UV excitable Ca2 indicators protein based Ca2 indicators conjugates of Ca2 indicators and for fluorescence based indicators of other metal ions i e Mg2 Zn2 review Indicators for Ca2 Mg2 Zn2 and Other Metal Ions Chapter 19 in the Molecular Probes Handbook For Research Use Only Not for human or animal therapeutic or diagnostic use
    https://www.bioz.com/result/ca2 indicator fluo 4/product/Thermo Fisher
    Average 99 stars, based on 53 article reviews
    Price from $9.99 to $1999.99
    ca2 indicator fluo 4 - by Bioz Stars, 2020-11
    99/100 stars

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    Related Articles

    Fluorescence:

    Article Title: Calcium Dynamics in Astrocytes During Cell Injury
    Article Snippet: .. Fluo4 highlights smaller fluctuations in intracellular Ca2+ with larger fluorescence intensity increases. .. With Fluo4 we observe large increases in Ca2+ throughout the entire cell in response to the same induced damage.

    Isolation:

    Article Title: Calcium Dynamics in Astrocytes During Cell Injury
    Article Snippet: .. This oscillation was also evident in isolated Ast1 astrocytes, however, it was lower in amplitude in primary astrocytes labeled with Fluo4 and Salsa6f. .. In comparing the Ca2+ intensity traces for Fluo4 and Salsa6f, we observed a greater number of peaks in Salsa6f labeled astrocytes vs. Fluo4 labeled astrocytes.

    Labeling:

    Article Title: Calcium Dynamics in Astrocytes During Cell Injury
    Article Snippet: .. This oscillation was also evident in isolated Ast1 astrocytes, however, it was lower in amplitude in primary astrocytes labeled with Fluo4 and Salsa6f. .. In comparing the Ca2+ intensity traces for Fluo4 and Salsa6f, we observed a greater number of peaks in Salsa6f labeled astrocytes vs. Fluo4 labeled astrocytes.

    Titration:

    Article Title: Highlighting curcumin-induced crosstalk between autophagy and apoptosis: A biochemical approach coupling impedancemetry, imaging, and flow cytometry
    Article Snippet: .. Ca2+ titration of Fluo4-AM was performed by reciprocal dilution of Ca2+ -free and Ca2+ -saturated buffers prepared by using a Ca2+ kit buffer (Invitrogen) on cells poisoned with DPBS supplemented with carbonyl cyanide m-chlorophenylhydrazone (mClCCCP, 10 µM) and ionomycin (5 µM) ( ). .. Most of the time double staining was done to assay simultaneously cell viability with propidium iodide (PI) at 1 µg.mL−1 final concentration.

    Concentration Assay:

    Article Title: Connexin-Mediated Signaling in Nonsensory Cells Is Crucial for the Development of Sensory Inner Hair Cells in the Mouse Cochlea
    Article Snippet: .. For calcium dye loading, acutely dissected preparations were incubated for 40 min at 37°C in DMEM/F12, supplemented with fluo-4 AM (final concentration 16 μm ; Thermo Fisher Scientific). .. The incubation medium contained also pluronic F-127 (0.1%, w/v, Sigma-Aldrich), and sulfinpyrazone (250 μm ) to prevent dye sequestration and secretion.

    Article Title: Store-operated Ca2+ entry and depolarization explain the anomalous behaviour of myometrial SR: Effects of SERCA inhibition on electrical activity, Ca2+ and force
    Article Snippet: .. For measurement of Ca2+ , the uterine strips were incubated in the membrane-permeant form of Fluo-4-AM, or Indo-1AM (15 μM, Invitrogen) dissolved in dimethyl sulphoxide premixed with Pluronic F127 (final concentration of 0.01%). ..

    Incubation:

    Article Title: Connexin-Mediated Signaling in Nonsensory Cells Is Crucial for the Development of Sensory Inner Hair Cells in the Mouse Cochlea
    Article Snippet: .. For calcium dye loading, acutely dissected preparations were incubated for 40 min at 37°C in DMEM/F12, supplemented with fluo-4 AM (final concentration 16 μm ; Thermo Fisher Scientific). .. The incubation medium contained also pluronic F-127 (0.1%, w/v, Sigma-Aldrich), and sulfinpyrazone (250 μm ) to prevent dye sequestration and secretion.

    Article Title: Store-operated Ca2+ entry and depolarization explain the anomalous behaviour of myometrial SR: Effects of SERCA inhibition on electrical activity, Ca2+ and force
    Article Snippet: .. For measurement of Ca2+ , the uterine strips were incubated in the membrane-permeant form of Fluo-4-AM, or Indo-1AM (15 μM, Invitrogen) dissolved in dimethyl sulphoxide premixed with Pluronic F127 (final concentration of 0.01%). ..

    other:

    Article Title: Tight coupling of astrocyte energy metabolism to synaptic activity revealed by genetically encoded FRET nanosensors in hippocampal tissue
    Article Snippet: Fluo-4-AM and BCECF were purchased from Invitrogen.

    Microscopy:

    Article Title: ALS/ FTD‐associated FUS activates GSK‐3β to disrupt the VAPB– PTPIP51 interaction and ER–mitochondria associations
    Article Snippet: .. Briefly, HEK293 cells co‐transfected with M3R and either empty vector or FUS was loaded with 2 μM Fluo4‐AM or Rhod2‐AM dye (Invitrogen) in external solution (145 mM NaCl, 2 mM KCl, 5 mM NaHCO3 , 1 mM MgCl2 , 2.5 mM CaCl2 , 10 mM glucose, 10 mM Na‐HEPES pH 7.25) containing 0.02% Pluronic‐F27 (Invitrogen) for 15 min at 37°C, followed by washing in external solution for 15 min. Fluo4 and Rhod2 fluorescences were timelapse recorded (1 s intervals) with MetaMorph (Molecular Dynamics) on an Axiovert S100 microscope (Zeiss) equipped with appropriate filter sets (Chroma Technology), a 40×/1.3NA Plan‐Neofluar objective (Zeiss) and a Photometrics Cascade‐II 512B EMCCD. ..

    Modification:

    Article Title: Loss of Cdk5 in breast cancer cells promotes ROS-mediated cell death through dysregulation of the mitochondrial permeability transition pore
    Article Snippet: .. Dulbecco’s modified Eagle’s medium (DMEM), heat-inactivated fetal bovine serum (FBS), EDTA-Trypsin, antibiotic-antimycotic, H2 -DCFDA, MitoSOX red, JC-1, MitoTracker green, and Fluo-4 AM were from Life Technologies Inc. Cdk5 (C-8), β-actin (C-19) and pro-caspase-9 (H-83) antibodies, and CsA and Mito-tempo were from Santa Cruz Biotech. .. Cleaved caspase-3 (5A1E), p-DRP S637 and DRP1 (4E11B11) antibodies were from Cell Signaling.

    Plasmid Preparation:

    Article Title: ALS/ FTD‐associated FUS activates GSK‐3β to disrupt the VAPB– PTPIP51 interaction and ER–mitochondria associations
    Article Snippet: .. Briefly, HEK293 cells co‐transfected with M3R and either empty vector or FUS was loaded with 2 μM Fluo4‐AM or Rhod2‐AM dye (Invitrogen) in external solution (145 mM NaCl, 2 mM KCl, 5 mM NaHCO3 , 1 mM MgCl2 , 2.5 mM CaCl2 , 10 mM glucose, 10 mM Na‐HEPES pH 7.25) containing 0.02% Pluronic‐F27 (Invitrogen) for 15 min at 37°C, followed by washing in external solution for 15 min. Fluo4 and Rhod2 fluorescences were timelapse recorded (1 s intervals) with MetaMorph (Molecular Dynamics) on an Axiovert S100 microscope (Zeiss) equipped with appropriate filter sets (Chroma Technology), a 40×/1.3NA Plan‐Neofluar objective (Zeiss) and a Photometrics Cascade‐II 512B EMCCD. ..

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  • 99
    Thermo Fisher ca2 indicator fluo 4
    Ca2 Indicator Fluo 4, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 53 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ca2 indicator fluo 4/product/Thermo Fisher
    Average 99 stars, based on 53 article reviews
    Price from $9.99 to $1999.99
    ca2 indicator fluo 4 - by Bioz Stars, 2020-11
    99/100 stars
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