Structured Review

Olympus ca2 imaging
Detection of Histamine-induced <t>Ca2+</t> Microdomains with Total Internal Reflection Fluorescence Microscope
Ca2 Imaging, supplied by Olympus, used in various techniques. Bioz Stars score: 92/100, based on 95 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ca2 imaging/product/Olympus
Average 92 stars, based on 95 article reviews
Price from $9.99 to $1999.99
ca2 imaging - by Bioz Stars, 2020-11
92/100 stars

Images

1) Product Images from "Cooperative and Stochastic Calcium Releases from Multiple Calcium Puff Sites Generate Calcium Microdomains in Intact HeLa Cells *"

Article Title: Cooperative and Stochastic Calcium Releases from Multiple Calcium Puff Sites Generate Calcium Microdomains in Intact HeLa Cells *

Journal: The Journal of Biological Chemistry

doi: 10.1074/jbc.M111.311399

Detection of Histamine-induced Ca2+ Microdomains with Total Internal Reflection Fluorescence Microscope
Figure Legend Snippet: Detection of Histamine-induced Ca2+ Microdomains with Total Internal Reflection Fluorescence Microscope

Techniques Used: Fluorescence, Microscopy

2) Product Images from "The Ca2+ Channel Subunit β2 Regulates Ca2+ Channel Abundance and Function in Inner Hair Cells and Is Required for Hearing"

Article Title: The Ca2+ Channel Subunit β2 Regulates Ca2+ Channel Abundance and Function in Inner Hair Cells and Is Required for Hearing

Journal: The Journal of neuroscience : the official journal of the Society for Neuroscience

doi: 10.1523/JNEUROSCI.1577-09.2009

CaV β 2 is abundant in IHCs and critical for IHC Ca2+ influx
Figure Legend Snippet: CaV β 2 is abundant in IHCs and critical for IHC Ca2+ influx

Techniques Used: Immunohistochemistry

3) Product Images from "Adenosine A2A Receptor Up-Regulates Retinal Wave Frequency via Starburst Amacrine Cells in the Developing Rat Retina"

Article Title: Adenosine A2A Receptor Up-Regulates Retinal Wave Frequency via Starburst Amacrine Cells in the Developing Rat Retina

Journal: PLoS ONE

doi: 10.1371/journal.pone.0095090

Live Ca2+ Imaging and Data Analysis
Figure Legend Snippet: Live Ca2+ Imaging and Data Analysis

Techniques Used: Imaging

4) Product Images from "The Ca2+ Channel Subunit β2 Regulates Ca2+ Channel Abundance and Function in Inner Hair Cells and Is Required for Hearing"

Article Title: The Ca2+ Channel Subunit β2 Regulates Ca2+ Channel Abundance and Function in Inner Hair Cells and Is Required for Hearing

Journal: The Journal of neuroscience : the official journal of the Society for Neuroscience

doi: 10.1523/JNEUROSCI.1577-09.2009

CaV β 2 is abundant in IHCs and critical for IHC Ca2+ influx
Figure Legend Snippet: CaV β 2 is abundant in IHCs and critical for IHC Ca2+ influx

Techniques Used: Immunohistochemistry

5) Product Images from "Identification of PGAM5 as a mammalian protein histidine phosphatase that plays a central role to negatively regulate CD4+ T cells"

Article Title: Identification of PGAM5 as a mammalian protein histidine phosphatase that plays a central role to negatively regulate CD4+ T cells

Journal: Molecular cell

doi: 10.1016/j.molcel.2016.06.021

PGAM5 does not directly dephosphorylate or inactivate KCa3.1 (A) GFP-tagged KCa3.1 was immuno-precipitated by GFP antibody from 293-KCa3.1 GFP cells and incubated with purified His-tagged PGAM5 (WT or H105A mutant) or His-tagged PHPT-1 for various time points as indicated. Samples were resolved by SDS/PAGE under basic conditions and immunoblotted with 3-pHis and GFP antibodies as indicated. (B) (i) Inside-out (I/O) patches were isolated from 293-KCa3.1 cells. Single channel activity was then recorded in I/O patches that were first incubated with GST-NDPK-B in the presence of 300 nM Ca2+ and GTP. This was followed by addition of His-tagged PGAM5 and His-tagged PHPT-1 to the same patch as indicated in the trace. (ii) Effect of NDPK-B, PGAM5 and PHPT-1 on the open channel probability, NPo. Bar graph represents KCa3.1 NPo as described in (Bi). All recordings in (B) were at +100 mV. Data are representative of three independent experiments. Data are shown as mean±SEM. Statistical significance was calculated using Student’s t test; *(p
Figure Legend Snippet: PGAM5 does not directly dephosphorylate or inactivate KCa3.1 (A) GFP-tagged KCa3.1 was immuno-precipitated by GFP antibody from 293-KCa3.1 GFP cells and incubated with purified His-tagged PGAM5 (WT or H105A mutant) or His-tagged PHPT-1 for various time points as indicated. Samples were resolved by SDS/PAGE under basic conditions and immunoblotted with 3-pHis and GFP antibodies as indicated. (B) (i) Inside-out (I/O) patches were isolated from 293-KCa3.1 cells. Single channel activity was then recorded in I/O patches that were first incubated with GST-NDPK-B in the presence of 300 nM Ca2+ and GTP. This was followed by addition of His-tagged PGAM5 and His-tagged PHPT-1 to the same patch as indicated in the trace. (ii) Effect of NDPK-B, PGAM5 and PHPT-1 on the open channel probability, NPo. Bar graph represents KCa3.1 NPo as described in (Bi). All recordings in (B) were at +100 mV. Data are representative of three independent experiments. Data are shown as mean±SEM. Statistical significance was calculated using Student’s t test; *(p

Techniques Used: Incubation, Purification, Mutagenesis, SDS Page, Isolation, Activity Assay

Related Articles

Patch Clamp:

Article Title: Target Cell-Specific Involvement of Presynaptic Mitochondria in Post-Tetanic Potentiation at Hippocampal Mossy Fiber Synapses
Article Snippet: .. For Ca2+ imaging at MFBs, whole-cell patch-clamp mode was attained on the soma of a GC under visual control using differential interference illumination in an upright microscope (BX51WI; Olympus, Tokyo, Japan) while perfusing a slice with normal aCSF (the composition is the same as low-calcium aCSF except 1.3 m m MgCl2 ,2.5 m m CaCl2 and without pyruvate and ascorbate). .. The whole-cell recordings were made using an EPC-10 amplifier (HEKA Elektronik, Lambrecht, Germany) with a pipette solution containing the following (in m m ): 140 K-gluconate, 5 di-Tris-phosphocreatin, 5 NaCl, 4 MgATP, 0.4 Na2 GTP, 15 HEPES, 2.5 Na-pyruvate at pH 7.3 (adjusted with KOH) together with Oregon Green 488 BAPTA-1 (OGB1) and/or Oregon Green 488 BAPTA-5N (OG5N).

Microscopy:

Article Title: Target Cell-Specific Involvement of Presynaptic Mitochondria in Post-Tetanic Potentiation at Hippocampal Mossy Fiber Synapses
Article Snippet: .. For Ca2+ imaging at MFBs, whole-cell patch-clamp mode was attained on the soma of a GC under visual control using differential interference illumination in an upright microscope (BX51WI; Olympus, Tokyo, Japan) while perfusing a slice with normal aCSF (the composition is the same as low-calcium aCSF except 1.3 m m MgCl2 ,2.5 m m CaCl2 and without pyruvate and ascorbate). .. The whole-cell recordings were made using an EPC-10 amplifier (HEKA Elektronik, Lambrecht, Germany) with a pipette solution containing the following (in m m ): 140 K-gluconate, 5 di-Tris-phosphocreatin, 5 NaCl, 4 MgATP, 0.4 Na2 GTP, 15 HEPES, 2.5 Na-pyruvate at pH 7.3 (adjusted with KOH) together with Oregon Green 488 BAPTA-1 (OGB1) and/or Oregon Green 488 BAPTA-5N (OG5N).

Article Title: The Ca2+ Channel Subunit β2 Regulates Ca2+ Channel Abundance and Function in Inner Hair Cells and Is Required for Hearing
Article Snippet: .. For Ca2+ imaging, an Olympus Fluoview 300 confocal scanner (R3896 P.M.T, Hamamatsu, as detector) mounted to a BX50WI microscope (0.9 numerical aperture, 60× water-immersion objective; Olympus) and a 50 mW, 488 nm solid state laser (Cyan; Newport-Spectraphysics) were used. .. Ca2+ microdomain overview images were acquired as background-subtracted averages of 12 96 × 96 pixel images acquired during six consecutive 390 ms depolarizations to −14 mV.

Article Title: Adenosine A2A Receptor Up-Regulates Retinal Wave Frequency via Starburst Amacrine Cells in the Developing Rat Retina
Article Snippet: .. Live Ca2+ imaging was performed on an upright fluorescent microscope (Olympus BX51WI) with a 20× water immersion objective. .. The transfected cells were identified by EGFP fluorescence (Ex 470/Em 525, Chroma #D41017).

Article Title: The Transduction Channel TRPM5 Is Gated by Intracellular Calcium in Taste Cells
Article Snippet: .. Ca2+ imaging was performed on an Olympus Optical IX71 microscope equipped with an LEP filter wheel (Ludl Electronic Products) and ORCA ER camera (Hamamatsu Photonics, Shizuoka, Japan). .. Cells transfected with TRPM5 fused to CFP were loaded through the patch pipette with the low-affinity Ca2+ indicator Fluo-5F (20 μ m ).

Article Title: Beta amyloid peptide plaques fail to alter evoked neuronal calcium signals in APP/PS1 Alzheimer's disease mice
Article Snippet: .. Ca2+ imaging within individual neurons was performed using a custom-made multiphoton-imaging system based on an upright Olympus BX51 microscope frame ( ). .. Individual neurons were filled with the Ca2+ indicator bis-fura-2 (50 μM) via the patch pipette.

Article Title: Identification of PGAM5 as a mammalian protein histidine phosphatase that plays a central role to negatively regulate CD4+ T cells
Article Snippet: .. Cells were then attached to a poly-L-lysine-coated coverslip for 20 min, and Ca2+ imaging was done with an IX81 epifluorescence microscope (Olympus) and OpenLab imaging software (Improvision), as described earlier ( ). .. For single-cell analysis, 340/380 nm fura-2 emission ratios of > 100 cells per experiment were analyzed.

Imaging:

Article Title: Target Cell-Specific Involvement of Presynaptic Mitochondria in Post-Tetanic Potentiation at Hippocampal Mossy Fiber Synapses
Article Snippet: .. For Ca2+ imaging at MFBs, whole-cell patch-clamp mode was attained on the soma of a GC under visual control using differential interference illumination in an upright microscope (BX51WI; Olympus, Tokyo, Japan) while perfusing a slice with normal aCSF (the composition is the same as low-calcium aCSF except 1.3 m m MgCl2 ,2.5 m m CaCl2 and without pyruvate and ascorbate). .. The whole-cell recordings were made using an EPC-10 amplifier (HEKA Elektronik, Lambrecht, Germany) with a pipette solution containing the following (in m m ): 140 K-gluconate, 5 di-Tris-phosphocreatin, 5 NaCl, 4 MgATP, 0.4 Na2 GTP, 15 HEPES, 2.5 Na-pyruvate at pH 7.3 (adjusted with KOH) together with Oregon Green 488 BAPTA-1 (OGB1) and/or Oregon Green 488 BAPTA-5N (OG5N).

Article Title: The Ca2+ Channel Subunit β2 Regulates Ca2+ Channel Abundance and Function in Inner Hair Cells and Is Required for Hearing
Article Snippet: .. For Ca2+ imaging, an Olympus Fluoview 300 confocal scanner (R3896 P.M.T, Hamamatsu, as detector) mounted to a BX50WI microscope (0.9 numerical aperture, 60× water-immersion objective; Olympus) and a 50 mW, 488 nm solid state laser (Cyan; Newport-Spectraphysics) were used. .. Ca2+ microdomain overview images were acquired as background-subtracted averages of 12 96 × 96 pixel images acquired during six consecutive 390 ms depolarizations to −14 mV.

Article Title: Cooperative and Stochastic Calcium Releases from Multiple Calcium Puff Sites Generate Calcium Microdomains in Intact HeLa Cells *
Article Snippet: .. Ca2+ imaging was carried out with an Olympus IX70-based TIRFM and an oil immersion 60× objective lens (numerical aperture (NA), 1.45). .. Fluorescence images were obtained by excitation at 488 nm with an Ar laser at room temperature (20–22 °C).

Article Title: Adenosine A2A Receptor Up-Regulates Retinal Wave Frequency via Starburst Amacrine Cells in the Developing Rat Retina
Article Snippet: .. Live Ca2+ imaging was performed on an upright fluorescent microscope (Olympus BX51WI) with a 20× water immersion objective. .. The transfected cells were identified by EGFP fluorescence (Ex 470/Em 525, Chroma #D41017).

Article Title: The Transduction Channel TRPM5 Is Gated by Intracellular Calcium in Taste Cells
Article Snippet: .. Ca2+ imaging was performed on an Olympus Optical IX71 microscope equipped with an LEP filter wheel (Ludl Electronic Products) and ORCA ER camera (Hamamatsu Photonics, Shizuoka, Japan). .. Cells transfected with TRPM5 fused to CFP were loaded through the patch pipette with the low-affinity Ca2+ indicator Fluo-5F (20 μ m ).

Article Title: Beta amyloid peptide plaques fail to alter evoked neuronal calcium signals in APP/PS1 Alzheimer's disease mice
Article Snippet: .. Ca2+ imaging within individual neurons was performed using a custom-made multiphoton-imaging system based on an upright Olympus BX51 microscope frame ( ). .. Individual neurons were filled with the Ca2+ indicator bis-fura-2 (50 μM) via the patch pipette.

Article Title: Identification of PGAM5 as a mammalian protein histidine phosphatase that plays a central role to negatively regulate CD4+ T cells
Article Snippet: .. Cells were then attached to a poly-L-lysine-coated coverslip for 20 min, and Ca2+ imaging was done with an IX81 epifluorescence microscope (Olympus) and OpenLab imaging software (Improvision), as described earlier ( ). .. For single-cell analysis, 340/380 nm fura-2 emission ratios of > 100 cells per experiment were analyzed.

Article Title: The Ca2+ Channel Subunit β2 Regulates Ca2+ Channel Abundance and Function in Inner Hair Cells and Is Required for Hearing
Article Snippet: .. We thank G. Matthews for providing fluorescently labeled CtBP2/RIBEYE-binding peptide, T. Frank for help with Ca2+ imaging and providing analysis routines, and T. Frank, A. Neef, A. Lee, D.Oliver, E. Reisinger, and L. Meier for comments on this manuscript. ..

Labeling:

Article Title: The Ca2+ Channel Subunit β2 Regulates Ca2+ Channel Abundance and Function in Inner Hair Cells and Is Required for Hearing
Article Snippet: .. We thank G. Matthews for providing fluorescently labeled CtBP2/RIBEYE-binding peptide, T. Frank for help with Ca2+ imaging and providing analysis routines, and T. Frank, A. Neef, A. Lee, D.Oliver, E. Reisinger, and L. Meier for comments on this manuscript. ..

Software:

Article Title: Identification of PGAM5 as a mammalian protein histidine phosphatase that plays a central role to negatively regulate CD4+ T cells
Article Snippet: .. Cells were then attached to a poly-L-lysine-coated coverslip for 20 min, and Ca2+ imaging was done with an IX81 epifluorescence microscope (Olympus) and OpenLab imaging software (Improvision), as described earlier ( ). .. For single-cell analysis, 340/380 nm fura-2 emission ratios of > 100 cells per experiment were analyzed.

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    Olympus ca2 imaginga flat transducer
    Ca2 Imaginga Flat Transducer, supplied by Olympus, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ca2 imaginga flat transducer/product/Olympus
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ca2 imaginga flat transducer - by Bioz Stars, 2020-11
    90/100 stars
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