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Carl Zeiss ca2 imaging
Th1 Cells Transfected with DN Trpm4 Have Decreased Peak and Mean <t>Ca2+</t> Levels and oscillations
Ca2 Imaging, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 92/100, based on 94 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ca2 imaging/product/Carl Zeiss
Average 92 stars, based on 94 article reviews
Price from $9.99 to $1999.99
ca2 imaging - by Bioz Stars, 2020-11
92/100 stars

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1) Product Images from "Trpm4 differentially regulates Th1 and Th2 function by altering calcium signaling and NFAT localization"

Article Title: Trpm4 differentially regulates Th1 and Th2 function by altering calcium signaling and NFAT localization

Journal: Journal of immunology (Baltimore, Md. : 1950)

doi: 10.4049/jimmunol.1000880

Th1 Cells Transfected with DN Trpm4 Have Decreased Peak and Mean Ca2+ Levels and oscillations
Figure Legend Snippet: Th1 Cells Transfected with DN Trpm4 Have Decreased Peak and Mean Ca2+ Levels and oscillations

Techniques Used: Transfection

Th2 Cells Transfected with DN Trpm4 Have Increased Mean Ca2+ Levels and oscillations
Figure Legend Snippet: Th2 Cells Transfected with DN Trpm4 Have Increased Mean Ca2+ Levels and oscillations

Techniques Used: Transfection

Related Articles

Inverted Microscopy:

Article Title: TRPV4 disrupts mitochondrial transport and causes axonal degeneration via a CaMKII-dependent elevation of intracellular Ca2+
Article Snippet: .. Ca2+ imaging was performed on a Zeiss AxioObserver.Z1 inverted microscope equipped with a Lambda DG-4 (Sutter Instrument Company) wavelength switcher. .. Prior to Ca2+ imaging of trigeminal neurons, a single channel GFP image was acquired to measure TRPV4 expression within individual neurons.

Microscopy:

Article Title: Trpm4 differentially regulates Th1 and Th2 function by altering calcium signaling and NFAT localization
Article Snippet: .. Ca2+ imaging was performed at 37°C using a temperature controlled environmental chamber on a Zeiss axiovert 200M microscope equipped with a xenon arc lamp. .. Fura-2 loaded cells were excited using 340 and 380 excitation filters (71000a set, Chroma Technology Corp) and a polychroic mirror (73100bs, Chroma Technology Corp).

Article Title: A severe defect in CRAC Ca2+ channel activation and altered K+ channel gating in T cells from immunodeficient patients
Article Snippet: .. Ca2+ imaging was done with a Zeiss Axiovert S200 epifluorescence microscope and OpenLab imaging software (Improvision) as described ( ). .. To actively deplete Ca2+ stores, T cells were incubated with 5 μg/ml biotinylated anti-CD3 antibody (UCHT1, BD Biosciences) for 10 min at 22–25°C followed by cross-linking with 5 μg/ml streptavidin (Pierce Chemical Co.).

Article Title: A C. elegans stretch receptor neuron revealed by a mechanosensitive TRP channel homologue
Article Snippet: .. Ca2+ imaging was done on an Axiovert 200 microscope (Zeiss) under a 40× objective. .. Images were acquired with a CoolSnap CCD camera (Roper) and processed by Ratiotool software (ISeeimaging).

Article Title: Saikosaponin d causes apoptotic death of cultured neocortical neurons by increasing membrane permeability and elevating intracellular Ca2+ concentration
Article Snippet: .. Ca2+ imaging was performed at room temperature using a ZEISS oberserver-Z1 microscope (Carl Zeiss AG, Jena, Germany). .. The imaging solution contained (in mM): NaCl 107, KCl 7.2, MgCl2 1.2, D-glucose 11.5, and HEPES 20 (pH 7.2).

Article Title: High-Throughput Screening of Tyrosine Kinase Inhibitor Cardiotoxicity with Human Induced Pluripotent Stem Cells
Article Snippet: .. Ca2+ imaging was conducted using a Zeiss LSM 510Meta confocal microscope (Carl Zeiss AG) and analyzed using Zen software. ..

Activity Assay:

Article Title: Assembly and Connection of Micropatterned Single Neurons for Neuronal Network Formation
Article Snippet: .. In Ca2+ imaging, culture medium was replaced with basal salt solution (described in ) to induce spontaneous neuronal activity, then imaged by real-time confocal microscopy (LSM710, Zeiss, Oberkochen, Germany). .. Time-lapse movies were taken with inverted microscopes (Olympus IX81) equipped with a humidified 37 °C incubator chamber containing 5% CO2 .

Imaging:

Article Title: Trpm4 differentially regulates Th1 and Th2 function by altering calcium signaling and NFAT localization
Article Snippet: .. Ca2+ imaging was performed at 37°C using a temperature controlled environmental chamber on a Zeiss axiovert 200M microscope equipped with a xenon arc lamp. .. Fura-2 loaded cells were excited using 340 and 380 excitation filters (71000a set, Chroma Technology Corp) and a polychroic mirror (73100bs, Chroma Technology Corp).

Article Title: A severe defect in CRAC Ca2+ channel activation and altered K+ channel gating in T cells from immunodeficient patients
Article Snippet: .. Ca2+ imaging was done with a Zeiss Axiovert S200 epifluorescence microscope and OpenLab imaging software (Improvision) as described ( ). .. To actively deplete Ca2+ stores, T cells were incubated with 5 μg/ml biotinylated anti-CD3 antibody (UCHT1, BD Biosciences) for 10 min at 22–25°C followed by cross-linking with 5 μg/ml streptavidin (Pierce Chemical Co.).

Article Title: TRPV4 disrupts mitochondrial transport and causes axonal degeneration via a CaMKII-dependent elevation of intracellular Ca2+
Article Snippet: .. Ca2+ imaging was performed on a Zeiss AxioObserver.Z1 inverted microscope equipped with a Lambda DG-4 (Sutter Instrument Company) wavelength switcher. .. Prior to Ca2+ imaging of trigeminal neurons, a single channel GFP image was acquired to measure TRPV4 expression within individual neurons.

Article Title: A C. elegans stretch receptor neuron revealed by a mechanosensitive TRP channel homologue
Article Snippet: .. Ca2+ imaging was done on an Axiovert 200 microscope (Zeiss) under a 40× objective. .. Images were acquired with a CoolSnap CCD camera (Roper) and processed by Ratiotool software (ISeeimaging).

Article Title: Assembly and Connection of Micropatterned Single Neurons for Neuronal Network Formation
Article Snippet: .. In Ca2+ imaging, culture medium was replaced with basal salt solution (described in ) to induce spontaneous neuronal activity, then imaged by real-time confocal microscopy (LSM710, Zeiss, Oberkochen, Germany). .. Time-lapse movies were taken with inverted microscopes (Olympus IX81) equipped with a humidified 37 °C incubator chamber containing 5% CO2 .

Article Title: Saikosaponin d causes apoptotic death of cultured neocortical neurons by increasing membrane permeability and elevating intracellular Ca2+ concentration
Article Snippet: .. Ca2+ imaging was performed at room temperature using a ZEISS oberserver-Z1 microscope (Carl Zeiss AG, Jena, Germany). .. The imaging solution contained (in mM): NaCl 107, KCl 7.2, MgCl2 1.2, D-glucose 11.5, and HEPES 20 (pH 7.2).

Article Title: High-Throughput Screening of Tyrosine Kinase Inhibitor Cardiotoxicity with Human Induced Pluripotent Stem Cells
Article Snippet: .. Ca2+ imaging was conducted using a Zeiss LSM 510Meta confocal microscope (Carl Zeiss AG) and analyzed using Zen software. ..

Article Title: Microglia Enhance Synapse Activity to Promote Local Network Synchronization
Article Snippet: .. Ca2+ imaging of L2/3 pyramidal neurons was conducted using a LSM 7 MP system (Zeiss) and a mode-locked Ti:sapphire Chameleon Ultra II laser (Coherent) tuned to 950 nm, with a 20× objective (XLPlan, NA of 1.0; Zeiss). .. Fluorescence was separated by a 570-nm dichroic mirror with 495–550 nm (green channel: for EGFP fluorescence detection) and 570–630 nm (red channel: for tdTomato fluorescence detection) emission filters and was collected using GaAsP photomultiplier tubes (Hamamatsu Photonics).

Confocal Microscopy:

Article Title: Assembly and Connection of Micropatterned Single Neurons for Neuronal Network Formation
Article Snippet: .. In Ca2+ imaging, culture medium was replaced with basal salt solution (described in ) to induce spontaneous neuronal activity, then imaged by real-time confocal microscopy (LSM710, Zeiss, Oberkochen, Germany). .. Time-lapse movies were taken with inverted microscopes (Olympus IX81) equipped with a humidified 37 °C incubator chamber containing 5% CO2 .

Software:

Article Title: A severe defect in CRAC Ca2+ channel activation and altered K+ channel gating in T cells from immunodeficient patients
Article Snippet: .. Ca2+ imaging was done with a Zeiss Axiovert S200 epifluorescence microscope and OpenLab imaging software (Improvision) as described ( ). .. To actively deplete Ca2+ stores, T cells were incubated with 5 μg/ml biotinylated anti-CD3 antibody (UCHT1, BD Biosciences) for 10 min at 22–25°C followed by cross-linking with 5 μg/ml streptavidin (Pierce Chemical Co.).

Article Title: High-Throughput Screening of Tyrosine Kinase Inhibitor Cardiotoxicity with Human Induced Pluripotent Stem Cells
Article Snippet: .. Ca2+ imaging was conducted using a Zeiss LSM 510Meta confocal microscope (Carl Zeiss AG) and analyzed using Zen software. ..

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    Carl Zeiss single cell ca2 imaginga zeiss axio observer z1 inverted microscope
    Single Cell Ca2 Imaginga Zeiss Axio Observer Z1 Inverted Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/single cell ca2 imaginga zeiss axio observer z1 inverted microscope/product/Carl Zeiss
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    single cell ca2 imaginga zeiss axio observer z1 inverted microscope - by Bioz Stars, 2020-11
    90/100 stars
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