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Waters Corporation c18 solid phase extraction spe cartridge
C18 Solid Phase Extraction Spe Cartridge, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 96/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/c18 solid phase extraction spe cartridge/product/Waters Corporation
Average 96 stars, based on 3 article reviews
Price from $9.99 to $1999.99
c18 solid phase extraction spe cartridge - by Bioz Stars, 2020-04
96/100 stars

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reversed-phase hplc
etoac
trifluoroacetic acid
tmt-labeled

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Related Articles

High Performance Liquid Chromatography:

Article Title: Melatonin mitigates cadmium phytotoxicity through modulation of phytochelatins biosynthesis, vacuolar sequestration, and antioxidant potential in Solanum lycopersicum L
Article Snippet: Paragraph title: Extraction and Quantification of Endogenous Melatonin by HPLC ... The supernatant was transferred to a C18 solid-phase extraction (SPE) cartridge (Waters, Milford, MA, USA) for the purification of melatonin.

Article Title: Estimation of angiotensin peptides in biological samples by LC/MS method
Article Snippet: The C18 solid phase extraction (SPE) cartridge was purchased from Waters Corporation (Milford, MA). .. Acetonitrile, deionized water, formic acid and all other solvents and chemicals were of HPLC grade from Sigma or Fisher Scientific.

Article Title: Determination of Xanthohumol in Hops, Food Supplements and Beers by HPLC
Article Snippet: The extracts yielded were diluted (when required) and filtered through a 0.22 µm PTFE membrane filters before HPLC analysis. .. For beer analyses, samples were first degassed by sonication for 1 h. Then, 10 mL of each sample was loaded on a C18 solid-phase extraction (SPE) cartridge (Waters Corporation, Dublin, Ireland) after conditioning with 5 mL methanol and 5 mL water.

Article Title: Protective mechanisms of melatonin against selenium toxicity in Brassica napus: insights into physiological traits, thiol biosynthesis and antioxidant machinery
Article Snippet: Paragraph title: Extraction and quantification of endogenous se and MT by HPLC-MS ... For the purification of MT, the supernatant was transferred to the C18 solid-phase extraction (SPE) cartridge (Waters, Milford, MA, USA).

Centrifugation:

Article Title: Melatonin Improved Anthocyanin Accumulation by Regulating Gene Expressions and Resulted in High Reactive Oxygen Species Scavenging Capacity in Cabbage
Article Snippet: After centrifugation at 11,417 g at 4°C for 15 min, the supernatants were collected and dried using nitrogen gas. .. The extracts were then dissolved in 5% methanol and purified using a C18 solid phase extraction (SPE) cartridge (Waters, Milford, MA, USA).

Article Title: Hydralazine targets cAMP-dependent protein kinase leading to sirtuin1/5 activation and lifespan extension in C. elegans
Article Snippet: Soluble proteins were separated by centrifugation for 30 min at 100,000 × g , and supernatant were used for further analysis. .. After quenching the reactions, TMT-labeled samples were mixed in equal ratio followed by clean up with C18 solid-phase extraction (SPE) cartridge (Sep-Pak, Waters).

Article Title: Identification of Candidate Cyclin-dependent kinase 1 (Cdk1) Substrates in Mitosis by Quantitative Phosphoproteomics *
Article Snippet: .. Trifluoroacetic acid was added to the digested peptide lysate to a final concentration of 0.25%, followed by centrifugation of the lysate at 3000 × g for 5 min. Peptides were desalted using a C18 solid-phase extraction (SPE) cartridge (SepPak, Waters, Milford, MA) and placed in a vacuum centrifuge for 45 min to evaporate the organic solvent. .. Phosphopeptide purification was performed using titanium dioxide microspheres essentially as described ( ).

Liquid Chromatography with Mass Spectroscopy:

Article Title: Protective mechanisms of melatonin against selenium toxicity in Brassica napus: insights into physiological traits, thiol biosynthesis and antioxidant machinery
Article Snippet: For the purification of MT, the supernatant was transferred to the C18 solid-phase extraction (SPE) cartridge (Waters, Milford, MA, USA). .. The obtained residue was dissolved in 0.5 mL of methanol (70%) and subjected to HPLC electrospray ionization/MS-MS analysis on an Agilent 6460 triple quad LC/MS with an Agilent-XDB18 column (2.1 mm × 150 mm, an Agilent Technologies, Frankfurt, Germany).

Chromatography:

Article Title: Rapid determination of multiple linear kinase substrate motifs by mass spectrometry
Article Snippet: The acidified lysates were desalted using a C18 solid-phase extraction (SPE) cartridge (Waters) and the eluate was lyophilized. .. Eight milligrams of lyophilized peptides were separated by strong-cation exchange chromatography as described ( ).

Protease Inhibitor:

Article Title: Hydralazine targets cAMP-dependent protein kinase leading to sirtuin1/5 activation and lifespan extension in C. elegans
Article Snippet: Worms were transferred to 1.5 ml tubes after adding PBS containing 0.08 NP-40 and protease inhibitor cocktail (Thermo Scientific) to a final volume of 70 µl. .. After quenching the reactions, TMT-labeled samples were mixed in equal ratio followed by clean up with C18 solid-phase extraction (SPE) cartridge (Sep-Pak, Waters).

Protein Concentration:

Article Title: Hydralazine targets cAMP-dependent protein kinase leading to sirtuin1/5 activation and lifespan extension in C. elegans
Article Snippet: Protein concentration was measured by BCA assay and proteins digested using S-Trap (Protifi) workflow following the vendor instruction. .. After quenching the reactions, TMT-labeled samples were mixed in equal ratio followed by clean up with C18 solid-phase extraction (SPE) cartridge (Sep-Pak, Waters).

Protein Extraction:

Article Title: Hydralazine targets cAMP-dependent protein kinase leading to sirtuin1/5 activation and lifespan extension in C. elegans
Article Snippet: Protein extraction was done with three freeze-thaw cycles using liquid nitrogen followed by sonication. .. After quenching the reactions, TMT-labeled samples were mixed in equal ratio followed by clean up with C18 solid-phase extraction (SPE) cartridge (Sep-Pak, Waters).

Labeling:

Article Title: Hydralazine targets cAMP-dependent protein kinase leading to sirtuin1/5 activation and lifespan extension in C. elegans
Article Snippet: Peptides were labeled using TMT- 10 plex (Thermo-Fisher Scientific) as described by the vendor. .. After quenching the reactions, TMT-labeled samples were mixed in equal ratio followed by clean up with C18 solid-phase extraction (SPE) cartridge (Sep-Pak, Waters).

Purification:

Article Title: Melatonin Improved Anthocyanin Accumulation by Regulating Gene Expressions and Resulted in High Reactive Oxygen Species Scavenging Capacity in Cabbage
Article Snippet: .. The extracts were then dissolved in 5% methanol and purified using a C18 solid phase extraction (SPE) cartridge (Waters, Milford, MA, USA). ..

Article Title: Melatonin mitigates cadmium phytotoxicity through modulation of phytochelatins biosynthesis, vacuolar sequestration, and antioxidant potential in Solanum lycopersicum L
Article Snippet: .. The supernatant was transferred to a C18 solid-phase extraction (SPE) cartridge (Waters, Milford, MA, USA) for the purification of melatonin. .. The residue was dissolved in 2 mL of the mobile phase for HPLC analysis as described ( ).

Article Title: Protective mechanisms of melatonin against selenium toxicity in Brassica napus: insights into physiological traits, thiol biosynthesis and antioxidant machinery
Article Snippet: .. For the purification of MT, the supernatant was transferred to the C18 solid-phase extraction (SPE) cartridge (Waters, Milford, MA, USA). .. The obtained residue was dissolved in 0.5 mL of methanol (70%) and subjected to HPLC electrospray ionization/MS-MS analysis on an Agilent 6460 triple quad LC/MS with an Agilent-XDB18 column (2.1 mm × 150 mm, an Agilent Technologies, Frankfurt, Germany).

Concentration Assay:

Article Title: Melatonin mitigates cadmium phytotoxicity through modulation of phytochelatins biosynthesis, vacuolar sequestration, and antioxidant potential in Solanum lycopersicum L
Article Snippet: Extraction and Quantification of Endogenous Melatonin by HPLC To measure the endogenous plant melatonin concentration, a direct sampling extraction procedure was used as described ( ) with modifications. .. The supernatant was transferred to a C18 solid-phase extraction (SPE) cartridge (Waters, Milford, MA, USA) for the purification of melatonin.

Article Title: Identification of Candidate Cyclin-dependent kinase 1 (Cdk1) Substrates in Mitosis by Quantitative Phosphoproteomics *
Article Snippet: .. Trifluoroacetic acid was added to the digested peptide lysate to a final concentration of 0.25%, followed by centrifugation of the lysate at 3000 × g for 5 min. Peptides were desalted using a C18 solid-phase extraction (SPE) cartridge (SepPak, Waters, Milford, MA) and placed in a vacuum centrifuge for 45 min to evaporate the organic solvent. .. Phosphopeptide purification was performed using titanium dioxide microspheres essentially as described ( ).

Article Title: Evaluation of HCD- and CID-type Fragmentation Within Their Respective Detection Platforms For Murine Phosphoproteomics *
Article Snippet: Trypsin digestion was terminated by adding trifluoroacetic acid to a final concentration of 0.2%. .. The digest was desalted over a 500 mg C18 solid-phase extraction (SPE) cartridge (Sep-Pak; Waters, Milford, MA) and lyophilized.

Incubation:

Article Title: Identification of Candidate Cyclin-dependent kinase 1 (Cdk1) Substrates in Mitosis by Quantitative Phosphoproteomics *
Article Snippet: The sample was removed and cooled to room temperature, and 15 m m iodoacetamide (Sigma) was added (final concentration) and the lysate was incubated in the dark for 60 mins. .. Trifluoroacetic acid was added to the digested peptide lysate to a final concentration of 0.25%, followed by centrifugation of the lysate at 3000 × g for 5 min. Peptides were desalted using a C18 solid-phase extraction (SPE) cartridge (SepPak, Waters, Milford, MA) and placed in a vacuum centrifuge for 45 min to evaporate the organic solvent.

Article Title: Evaluation of HCD- and CID-type Fragmentation Within Their Respective Detection Platforms For Murine Phosphoproteomics *
Article Snippet: Each 10-mg aliquot of lysate was incubated overnight with 150 μg of sequencing grade trypsin (Promega, Madison, WI). .. The digest was desalted over a 500 mg C18 solid-phase extraction (SPE) cartridge (Sep-Pak; Waters, Milford, MA) and lyophilized.

Mass Spectrometry:

Article Title: Melatonin Improved Anthocyanin Accumulation by Regulating Gene Expressions and Resulted in High Reactive Oxygen Species Scavenging Capacity in Cabbage
Article Snippet: The extracts were then dissolved in 5% methanol and purified using a C18 solid phase extraction (SPE) cartridge (Waters, Milford, MA, USA). .. The extract was subsequently filtered through a 0.22 μm PTFE syringe filter before UHPLC-ESI-MS/ MS analysis.

Article Title: Hydralazine targets cAMP-dependent protein kinase leading to sirtuin1/5 activation and lifespan extension in C. elegans
Article Snippet: After quenching the reactions, TMT-labeled samples were mixed in equal ratio followed by clean up with C18 solid-phase extraction (SPE) cartridge (Sep-Pak, Waters). .. MS analysis was performed using a Fusion Orbitrap Lumos mass spectrometer (Thermo Scientific) connected to a Dionex Ultimate 3000 UHPLC (Thermo Scientific).

Article Title: Determination of Xanthohumol in Hops, Food Supplements and Beers by HPLC
Article Snippet: For beer analyses, samples were first degassed by sonication for 1 h. Then, 10 mL of each sample was loaded on a C18 solid-phase extraction (SPE) cartridge (Waters Corporation, Dublin, Ireland) after conditioning with 5 mL methanol and 5 mL water. .. Fractions yielded from samples G and MS were evaporated to dryness under a stream of nitrogen at 40 °C, using an evaporator system Labconco Rapid Vertex-Evaporator (Labconco Corporation, Kansas City, MO, USA) to concentrate ten times, and fractions yielded from samples M and B were concentrated 2 times under the same procedure.

BIA-KA:

Article Title: Hydralazine targets cAMP-dependent protein kinase leading to sirtuin1/5 activation and lifespan extension in C. elegans
Article Snippet: Protein concentration was measured by BCA assay and proteins digested using S-Trap (Protifi) workflow following the vendor instruction. .. After quenching the reactions, TMT-labeled samples were mixed in equal ratio followed by clean up with C18 solid-phase extraction (SPE) cartridge (Sep-Pak, Waters).

Article Title: Identification of Candidate Cyclin-dependent kinase 1 (Cdk1) Substrates in Mitosis by Quantitative Phosphoproteomics *
Article Snippet: Approximately 200 μl of lysate was removed to measure total protein using a BCA protein assay kit (Thermo Fisher Scientific, Grand Island, NY). .. Trifluoroacetic acid was added to the digested peptide lysate to a final concentration of 0.25%, followed by centrifugation of the lysate at 3000 × g for 5 min. Peptides were desalted using a C18 solid-phase extraction (SPE) cartridge (SepPak, Waters, Milford, MA) and placed in a vacuum centrifuge for 45 min to evaporate the organic solvent.

Sequencing:

Article Title: Rapid determination of multiple linear kinase substrate motifs by mass spectrometry
Article Snippet: One μg sequencing-grade Gluc-C per 75μg total protein and one μg sequencing-grade chymotrypsin per 100μg total protein was added, and incubated overnight at 25°C. .. The acidified lysates were desalted using a C18 solid-phase extraction (SPE) cartridge (Waters) and the eluate was lyophilized.

Article Title: Evaluation of HCD- and CID-type Fragmentation Within Their Respective Detection Platforms For Murine Phosphoproteomics *
Article Snippet: Each 10-mg aliquot of lysate was incubated overnight with 150 μg of sequencing grade trypsin (Promega, Madison, WI). .. The digest was desalted over a 500 mg C18 solid-phase extraction (SPE) cartridge (Sep-Pak; Waters, Milford, MA) and lyophilized.

Sonication:

Article Title: Hydralazine targets cAMP-dependent protein kinase leading to sirtuin1/5 activation and lifespan extension in C. elegans
Article Snippet: Protein extraction was done with three freeze-thaw cycles using liquid nitrogen followed by sonication. .. After quenching the reactions, TMT-labeled samples were mixed in equal ratio followed by clean up with C18 solid-phase extraction (SPE) cartridge (Sep-Pak, Waters).

Article Title: Determination of Xanthohumol in Hops, Food Supplements and Beers by HPLC
Article Snippet: .. For beer analyses, samples were first degassed by sonication for 1 h. Then, 10 mL of each sample was loaded on a C18 solid-phase extraction (SPE) cartridge (Waters Corporation, Dublin, Ireland) after conditioning with 5 mL methanol and 5 mL water. ..

Lysis:

Article Title: Identification of Candidate Cyclin-dependent kinase 1 (Cdk1) Substrates in Mitosis by Quantitative Phosphoproteomics *
Article Snippet: Paragraph title: Lysis and Digestion ... Trifluoroacetic acid was added to the digested peptide lysate to a final concentration of 0.25%, followed by centrifugation of the lysate at 3000 × g for 5 min. Peptides were desalted using a C18 solid-phase extraction (SPE) cartridge (SepPak, Waters, Milford, MA) and placed in a vacuum centrifuge for 45 min to evaporate the organic solvent.

Sampling:

Article Title: Melatonin mitigates cadmium phytotoxicity through modulation of phytochelatins biosynthesis, vacuolar sequestration, and antioxidant potential in Solanum lycopersicum L
Article Snippet: Extraction and Quantification of Endogenous Melatonin by HPLC To measure the endogenous plant melatonin concentration, a direct sampling extraction procedure was used as described ( ) with modifications. .. The supernatant was transferred to a C18 solid-phase extraction (SPE) cartridge (Waters, Milford, MA, USA) for the purification of melatonin.

De-Phosphorylation Assay:

Article Title: Rapid determination of multiple linear kinase substrate motifs by mass spectrometry
Article Snippet: The acidified lysates were desalted using a C18 solid-phase extraction (SPE) cartridge (Waters) and the eluate was lyophilized. .. Dephosphorylation was repeated with λ-phosphatase (500 units/mg peptide) in 50mM Hepes, pH7.5, 100mM NaCl, 2mM DTT, overnight at 37°C, and desalted using a C18 solid-phase extraction (SPE) cartridge and the eluate was lyophilized.

Article Title: Rapid determination of multiple linear kinase substrate motifs by mass spectrometry
Article Snippet: .. Dephosphorylation was repeated with λ-phosphatase (500 units/mg peptide) in 50mM Hepes, pH7.5, 100mM NaCl, 2mM DTT, overnight at 37°C, and desalted using a C18 solid-phase extraction (SPE) cartridge and the eluate was lyophilized. .. Eight milligrams of lyophilized peptides were separated by strong-cation exchange chromatography as described ( ).

Injection:

Article Title: Determination of Xanthohumol in Hops, Food Supplements and Beers by HPLC
Article Snippet: For beer analyses, samples were first degassed by sonication for 1 h. Then, 10 mL of each sample was loaded on a C18 solid-phase extraction (SPE) cartridge (Waters Corporation, Dublin, Ireland) after conditioning with 5 mL methanol and 5 mL water. .. A portion of the extract was filtered with a 0.22 µm PTFE membrane filter before HPLC injection.

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    Waters Corporation solid phase extraction spe cartridges
    Solid Phase Extraction Spe Cartridges, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 94/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/solid phase extraction spe cartridges/product/Waters Corporation
    Average 94 stars, based on 15 article reviews
    Price from $9.99 to $1999.99
    solid phase extraction spe cartridges - by Bioz Stars, 2020-04
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      Buy from Supplier

    94
    Waters Corporation sep pak c18 solid phase extraction spe cartridges
    Sep Pak C18 Solid Phase Extraction Spe Cartridges, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sep pak c18 solid phase extraction spe cartridges/product/Waters Corporation
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    Price from $9.99 to $1999.99
    sep pak c18 solid phase extraction spe cartridges - by Bioz Stars, 2020-04
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    86
    Waters Corporation solid phase extraction spe c18 cartridge
    Solid Phase Extraction Spe C18 Cartridge, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    solid phase extraction spe c18 cartridge - by Bioz Stars, 2020-04
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      Buy from Supplier

    93
    Waters Corporation c18 solid phase extraction spe cartridges
    C18 Solid Phase Extraction Spe Cartridges, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/c18 solid phase extraction spe cartridges/product/Waters Corporation
    Average 93 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    c18 solid phase extraction spe cartridges - by Bioz Stars, 2020-04
    93/100 stars
      Buy from Supplier

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