Structured Review

Waters Corporation c18 seppak light
C18 Seppak Light, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 80/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/c18 seppak light/product/Waters Corporation
Average 80 stars, based on 2 article reviews
Price from $9.99 to $1999.99
c18 seppak light - by Bioz Stars, 2020-04
80/100 stars

Related Products / Commonly Used Together

etoh
naoac buffer
cu cu-ets
ionic 64 cu
200 mci

Images

Related Articles

Radioactivity:

Article Title: Species Dependence of [64Cu]Cu-Bis(thiosemicarbazone) Radiopharmaceutical Binding to Serum Albumins
Article Snippet: The [64 Cu]copper-bis(thiosemicarbazone) solutions were then diluted to < 5% EtOH with water and loaded onto a C18 SepPak Light® (Waters, Milford, MA) solid-phase extraction cartridge (previously conditioned with 5mL EtOH, followed by 10mL water). .. Radiochemical yields were typically ∼90% in the combined ethanol fractions for these three compounds, with 60-70% of the radioactivity appearing in the most concentrated of the 0.1 mL fractions. (The “lost radioactivity” remained in the reaction vial or bound to the SepPak cartridge.)

Labeling:

Article Title: In Vitro and In Vivo Evaluation of Bifunctional Bisthiosemicarbazone 64Cu-Complexes for the Positron Emission Tomography Imaging of Hypoxia
Article Snippet: A total of 200 μ L (20 μ g) of this solution was then added to 200 μ L (10 mCi) of 64 Cu in NaOAc buffer (0.05 M, pH 5.5), which was typically supplied at a specific activity of around 200 mCi/ μ g. This solution was then stirred for 30 min at 37 °C, after which it was loaded onto a C18 SepPak-Light (Waters Corporation, Milford, MA), which had been pre-equilibrated with 5 mL of ethanol and 5 mL of water. .. The labeled complexes were then eluted in 350 μ L of ethanol (following a 150 μ L ethanol elution for the void volume).

Purification:

Article Title: Elucidation of the Human Serum Albumin (HSA) Binding Site for the Cu-PTSM and Cu-ATSM Radiopharmaceuticals
Article Snippet: .. The [64 Cu]Cu-PTSM, [64 Cu]Cu-ATSM, and [64 Cu]Cu-ETS radiopharmaceuticals were prepared as described previously, and purified to remove traces of ionic 64 Cu using C18 SepPak Light (Waters, Milford, MA) solid-phase extraction cartridges. .. Following recovery in EtOH, the radiochemical purity of each chelate exceeded 99% (silica gel TLC developed with ethanol).

Article Title: Nitroimidazole conjugates of bis(thiosemicarbazonato)64Cu(II) – Potential combination agents for the PET imaging of hypoxia
Article Snippet: This solution was then stirred for 30 min at 37 °C, after which it was loaded onto a C18 SepPak-Light® (Waters Corporation, Milford, MA), which had been conditioned with 5 mL of ethanol and 5 mL of water. .. The radiochemical yield for all radiolabelling reactions was 80–85% and were all purified to > 95% purity.

Concentration Assay:

Article Title: Nitroimidazole conjugates of bis(thiosemicarbazonato)64Cu(II) – Potential combination agents for the PET imaging of hypoxia
Article Snippet: The ligand was dissolved in DMSO to give an overall concentration of 0.1 mg/mL. .. This solution was then stirred for 30 min at 37 °C, after which it was loaded onto a C18 SepPak-Light® (Waters Corporation, Milford, MA), which had been conditioned with 5 mL of ethanol and 5 mL of water.

Article Title: In Vitro and In Vivo Evaluation of Bifunctional Bisthiosemicarbazone 64Cu-Complexes for the Positron Emission Tomography Imaging of Hypoxia
Article Snippet: The ligands or zinc complexes were dissolved in DMSO to give an overall concentration of 0.1 mg/mL. .. A total of 200 μ L (20 μ g) of this solution was then added to 200 μ L (10 mCi) of 64 Cu in NaOAc buffer (0.05 M, pH 5.5), which was typically supplied at a specific activity of around 200 mCi/ μ g. This solution was then stirred for 30 min at 37 °C, after which it was loaded onto a C18 SepPak-Light (Waters Corporation, Milford, MA), which had been pre-equilibrated with 5 mL of ethanol and 5 mL of water.

Article Title: Species Dependence of [64Cu]Cu-Bis(thiosemicarbazone) Radiopharmaceutical Binding to Serum Albumins
Article Snippet: No-carrier-added 67 Cu (1.6 mCi; 59.2 MBq) was obtained as 67 Cu2+ in 0.1N HCl (0.05 mL) at a 36 ppm concentration of trace metals (Cu, Pb, Fe, Ni, Ag, Zn) from Trace Radiochemicals, Inc. (Denton, TX). .. The [64 Cu]copper-bis(thiosemicarbazone) solutions were then diluted to < 5% EtOH with water and loaded onto a C18 SepPak Light® (Waters, Milford, MA) solid-phase extraction cartridge (previously conditioned with 5mL EtOH, followed by 10mL water).

Thin Layer Chromatography:

Article Title: Elucidation of the Human Serum Albumin (HSA) Binding Site for the Cu-PTSM and Cu-ATSM Radiopharmaceuticals
Article Snippet: The [64 Cu]Cu-PTSM, [64 Cu]Cu-ATSM, and [64 Cu]Cu-ETS radiopharmaceuticals were prepared as described previously, and purified to remove traces of ionic 64 Cu using C18 SepPak Light (Waters, Milford, MA) solid-phase extraction cartridges. .. Following recovery in EtOH, the radiochemical purity of each chelate exceeded 99% (silica gel TLC developed with ethanol).

Article Title: Species Dependence of [64Cu]Cu-Bis(thiosemicarbazone) Radiopharmaceutical Binding to Serum Albumins
Article Snippet: The [64 Cu]copper-bis(thiosemicarbazone) solutions were then diluted to < 5% EtOH with water and loaded onto a C18 SepPak Light® (Waters, Milford, MA) solid-phase extraction cartridge (previously conditioned with 5mL EtOH, followed by 10mL water). .. The radiochemical purity of each product was determined by thin layer chromatography using silica gel plates and 100% EtOH as the mobile phase and always exceeded 99% ( Rf values of 0.86, 0.84, 0.87, and 0 for [64 Cu]Cu-PTSM, [64 Cu]Cu-ETS, [64 Cu]Cu-ATSM, and ionic [64 Cu]copper(II) acetate, respectively).

Activity Assay:

Article Title: Nitroimidazole conjugates of bis(thiosemicarbazonato)64Cu(II) – Potential combination agents for the PET imaging of hypoxia
Article Snippet: A total of 200 μL (20 μg) of this solution was then added to 200 μL (10 mCi) of 64 Cu in chelexed NaOAc buffer (0.1 M), which was typically supplied at a specific activity of around 200 mCi/μg. .. This solution was then stirred for 30 min at 37 °C, after which it was loaded onto a C18 SepPak-Light® (Waters Corporation, Milford, MA), which had been conditioned with 5 mL of ethanol and 5 mL of water.

Article Title: In Vitro and In Vivo Evaluation of Bifunctional Bisthiosemicarbazone 64Cu-Complexes for the Positron Emission Tomography Imaging of Hypoxia
Article Snippet: .. A total of 200 μ L (20 μ g) of this solution was then added to 200 μ L (10 mCi) of 64 Cu in NaOAc buffer (0.05 M, pH 5.5), which was typically supplied at a specific activity of around 200 mCi/ μ g. This solution was then stirred for 30 min at 37 °C, after which it was loaded onto a C18 SepPak-Light (Waters Corporation, Milford, MA), which had been pre-equilibrated with 5 mL of ethanol and 5 mL of water. ..

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 87
    Waters Corporation tc18 light reverse phase silica cartridge
    Tc18 Light Reverse Phase Silica Cartridge, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 87/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tc18 light reverse phase silica cartridge/product/Waters Corporation
    Average 87 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tc18 light reverse phase silica cartridge - by Bioz Stars, 2020-04
    87/100 stars
      Buy from Supplier

    94
    Waters Corporation solid phase extraction cartridges
    Solid Phase Extraction Cartridges, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 94/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/solid phase extraction cartridges/product/Waters Corporation
    Average 94 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    solid phase extraction cartridges - by Bioz Stars, 2020-04
    94/100 stars
      Buy from Supplier

    93
    Waters Corporation c18 light seppak cartridge
    C18 Light Seppak Cartridge, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/c18 light seppak cartridge/product/Waters Corporation
    Average 93 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    c18 light seppak cartridge - by Bioz Stars, 2020-04
    93/100 stars
      Buy from Supplier

    Image Search Results