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Waters Corporation c18 seppak classic cartridge
C18 Seppak Classic Cartridge, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/c18 seppak classic cartridge/product/Waters Corporation
Average 94 stars, based on 3 article reviews
Price from $9.99 to $1999.99
c18 seppak classic cartridge - by Bioz Stars, 2020-04
94/100 stars

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High Performance Liquid Chromatography:

Article Title: Age-Related Change in 5-HT6 Receptor Availability in Healthy Male Volunteers Measured with 11C-GSK215083 PET
Article Snippet: The reaction mixture was cooled to 50°C, diluted with 1 ml of deionized water, and injected onto the semipreparative high-performance liquid chromatography column (Gemini C18, 5 μm, 10 × 250 mm; Phenomenex Inc.) eluting at a flow rate of 4 mL/min with a mixture of 40% acetonitrile and 60% 0.05 M ammonium formate adjusted to pH 8.1–8.4 using saturated ammonium hydroxide. .. The radioactivity fraction eluting between 18 and 19 min was collected, diluted with 50 mL of water, and loaded onto a C18 SepPak classic cartridge (Waters).

Centrifugation:

Article Title: Transcription factor HAT1 is a substrate of SnRK2.3 kinase and negatively regulates ABA synthesis and signaling in Arabidopsis responding to drought
Article Snippet: .. After centrifugation (4000 r/min for 10 min, 4°C), the supernatant was passed through a C18-SepPak classic cartridge (Waters, Milford, USA) [ ]. .. ABA content measurement was performed by using a Plant hormone abscisic acid (ABA) ELISA Kit (BIOSAMITE, CK- ).

Article Title: Dinor-oxo-phytodienoic acid: A new hexadecanoid signal in the jasmonate family
Article Snippet: .. Ice-cold water (1.5 ml) was added and, after centrifugation, the supernatant was passed through a C18-SepPak classic cartridge (Waters), which had been prewashed with methanol followed by methanol/water (70:30, vol/vol). ..

Radioactivity:

Article Title: Age-Related Change in 5-HT6 Receptor Availability in Healthy Male Volunteers Measured with 11C-GSK215083 PET
Article Snippet: .. The radioactivity fraction eluting between 18 and 19 min was collected, diluted with 50 mL of water, and loaded onto a C18 SepPak classic cartridge (Waters). ..

Mass Spectrometry:

Article Title: Transcription factor HAT1 is a substrate of SnRK2.3 kinase and negatively regulates ABA synthesis and signaling in Arabidopsis responding to drought
Article Snippet: Arabidopsis seedlings grown on 1/2 MS medium plates were transferred to 1/2 MS liquid medium (CK) and 1/2MS liquid medium containing 15% PEG (drought stress treatment) for indicated time, and then the seedlings were harvested for gene expression analysis or ABA content assay. .. After centrifugation (4000 r/min for 10 min, 4°C), the supernatant was passed through a C18-SepPak classic cartridge (Waters, Milford, USA) [ ].

Incubation:

Article Title: Transcription factor HAT1 is a substrate of SnRK2.3 kinase and negatively regulates ABA synthesis and signaling in Arabidopsis responding to drought
Article Snippet: For ABA content assay, 0.5g 12-day-old seedlings with or without 15% PEG treatment were homogenized in 2 mL of 80% methanol, and incubated with additional 3 mL of 80% methanol overnight at 4°C. .. After centrifugation (4000 r/min for 10 min, 4°C), the supernatant was passed through a C18-SepPak classic cartridge (Waters, Milford, USA) [ ].

Enzyme-linked Immunosorbent Assay:

Article Title: Transcription factor HAT1 is a substrate of SnRK2.3 kinase and negatively regulates ABA synthesis and signaling in Arabidopsis responding to drought
Article Snippet: After centrifugation (4000 r/min for 10 min, 4°C), the supernatant was passed through a C18-SepPak classic cartridge (Waters, Milford, USA) [ ]. .. ABA content measurement was performed by using a Plant hormone abscisic acid (ABA) ELISA Kit (BIOSAMITE, CK- ).

Quantitation Assay:

Article Title: Dinor-oxo-phytodienoic acid: A new hexadecanoid signal in the jasmonate family
Article Snippet: For analysis and quantitation of fatty acids, leaf material was harvested, weighed, and immediately frozen in liquid nitrogen. .. Ice-cold water (1.5 ml) was added and, after centrifugation, the supernatant was passed through a C18-SepPak classic cartridge (Waters), which had been prewashed with methanol followed by methanol/water (70:30, vol/vol).

Activity Assay:

Article Title: Age-Related Change in 5-HT6 Receptor Availability in Healthy Male Volunteers Measured with 11C-GSK215083 PET
Article Snippet: The radioactivity fraction eluting between 18 and 19 min was collected, diluted with 50 mL of water, and loaded onto a C18 SepPak classic cartridge (Waters). .. Purity and molar activity were determined using an analytic reversed-phase high-performance liquid chromatography column (Luna C18(2), 5 μm, 250 × 4.6 mm; Phenomenex) eluting with a mixture of 40% acetonitrile and 60% 0.1 M ammonium formate at a flow rate of 1.5 mL/min.

Expressing:

Article Title: Transcription factor HAT1 is a substrate of SnRK2.3 kinase and negatively regulates ABA synthesis and signaling in Arabidopsis responding to drought
Article Snippet: Arabidopsis seedlings grown on 1/2 MS medium plates were transferred to 1/2 MS liquid medium (CK) and 1/2MS liquid medium containing 15% PEG (drought stress treatment) for indicated time, and then the seedlings were harvested for gene expression analysis or ABA content assay. .. After centrifugation (4000 r/min for 10 min, 4°C), the supernatant was passed through a C18-SepPak classic cartridge (Waters, Milford, USA) [ ].

Injection:

Article Title: Age-Related Change in 5-HT6 Receptor Availability in Healthy Male Volunteers Measured with 11C-GSK215083 PET
Article Snippet: The reaction mixture was cooled to 50°C, diluted with 1 ml of deionized water, and injected onto the semipreparative high-performance liquid chromatography column (Gemini C18, 5 μm, 10 × 250 mm; Phenomenex Inc.) eluting at a flow rate of 4 mL/min with a mixture of 40% acetonitrile and 60% 0.05 M ammonium formate adjusted to pH 8.1–8.4 using saturated ammonium hydroxide. .. The radioactivity fraction eluting between 18 and 19 min was collected, diluted with 50 mL of water, and loaded onto a C18 SepPak classic cartridge (Waters).

Flow Cytometry:

Article Title: Age-Related Change in 5-HT6 Receptor Availability in Healthy Male Volunteers Measured with 11C-GSK215083 PET
Article Snippet: The reaction mixture was cooled to 50°C, diluted with 1 ml of deionized water, and injected onto the semipreparative high-performance liquid chromatography column (Gemini C18, 5 μm, 10 × 250 mm; Phenomenex Inc.) eluting at a flow rate of 4 mL/min with a mixture of 40% acetonitrile and 60% 0.05 M ammonium formate adjusted to pH 8.1–8.4 using saturated ammonium hydroxide. .. The radioactivity fraction eluting between 18 and 19 min was collected, diluted with 50 mL of water, and loaded onto a C18 SepPak classic cartridge (Waters).

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    Waters Corporation classic c18 seppak cartridge
    Classic C18 Seppak Cartridge, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/classic c18 seppak cartridge/product/Waters Corporation
    Average 92 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    classic c18 seppak cartridge - by Bioz Stars, 2020-04
    92/100 stars
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