Structured Review

Waters Corporation c18 seppak cartridges
C18 Seppak Cartridges, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 95/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/c18 seppak cartridges/product/Waters Corporation
Average 95 stars, based on 19 article reviews
Price from $9.99 to $1999.99
c18 seppak cartridges - by Bioz Stars, 2020-04
95/100 stars

Images

Related Articles

Centrifugation:

Article Title: Identification of putative biomarkers for HIV-associated neurocognitive impairment in the CSF of HIV-infected patients under cART therapy determined by mass spectrometry
Article Snippet: Nine molar urea in triethylammonium bicarbonate (TEABC) were added to the CSF samples; then, they were incubated at 25 °C for 1 h. The samples were filtered using 10 kDa cutoff Amicon Ultra 0.5-mL tubes by centrifugation at 5,000 g for 15 min. .. To increase labeling affinity, the samples were acidified with 10 μL of 20 % trifluoroacetic acid (TFA) (Thermo Scientific) followed by cleanup with C18 SepPak cartridges (Waters).

Article Title: Combining LOPIT with differential ultracentrifugation for high-resolution spatial proteomics
Article Snippet: After labelling and quenching, peptides were pooled into 10-plexes and reduced to dryness by vacuum centrifugation. .. The combined, TMT-labelled samples were then cleaned using C18 SepPak cartridges (Waters) to remove salts and other substances which could interfere with downstream peptide processing.

Article Title: Cell Surface Proteome of Dental Pulp Stem Cells Identified by Label-Free Mass Spectrometry
Article Snippet: The reagent was eliminated by centrifugation for 15 min at 14,000 x g , washed twice with 100 μL 8 M urea, 0.1 M Tris-HCl, pH 8.5 followed by two washes with 100 μl 50 mM ammonium bicarbonate. .. Peptides were desalted using C18-SepPak cartridges (WAT023590, Waters, Milford, MA, USA).

Mass Spectrometry:

Article Title: High Boron-loaded DNA-Oligomers as Potential Boron Neutron Capture Therapy and Antisense Oligonucleotide Dual-Action Anticancer Agents ‡
Article Snippet: The collected oligonucleotide fractions were desalted on C18 SepPak cartridges (Waters Corp., Milford, MA, USA). .. The molecular mass of all the synthesized oligonucleotides was confirmed by MALDI-TOF mass spectrometry ( ) and their purity by polyacrylamide gel electrophoresis (PAGE) analysis ( ).

Article Title: Phosphoproteome Analysis Reveals Differential Mode of Action of Sorafenib in Wildtype and Mutated FLT3 Acute Myeloid Leukemia (AML) Cells *
Article Snippet: Tryptic peptides were desalted using reversed-phase 500 mg C18 SepPak cartridges (Waters, Eschborn, Germany) and fractionated by SCX chromatography (250 × 9.4 mm polySULFOETHYL A column, 200 Å pore size, 5 μm particle size (PolyLC)) operated with ÄKTA explorer system (GE Healthcare, München, Germany) as described previously ( ). .. Phosphopeptide-enriched samples were loaded onto a reverse phase analytical column (packed in-house with C18 beads), resolved by an acetonitrile gradient using an Easy nLC system (Thermo Fisher Scientific, Waltham, MA) and directly electrosprayed via a nanoelectrospray ion source into an LTQ Orbitrap Velos mass spectrometer (Thermo Fisher Scientific) ( , ).

Article Title: Recombinant production of rhesus ?-defensin-1 (RTD-1) using a bacterial expression system
Article Snippet: Both the soluble cell lysate and solubilized cell lysate pellet were extracted using C18 SepPak cartridges (Waters, Milford, MA) with elution in MeCN:H2 O (3:2 vol.) containing 0.1% TFA and lyophilized. .. The samples were dissolved in H2 O containing 0.1% formic acid and analyzed by HPLC-tandem mass spectrometry using a C18-HPLC column (5 mm, 2.1 × 100 mm), and H2 O-MeCN buffers containing 0.1% formic acid as mobile phase.

Article Title: Biosynthesis and antimicrobial evaluation of backbone-cyclized alpha-defensins#
Article Snippet: Both the soluble cell lysate and solubilized cell lysate pellet were extracted using C18 SepPak cartridges (Waters, Milford, MA) with elution in MeCN:H2 O (3:2 vol.) containing 0.1% TFA. .. The samples were analyzed by HPLC-tandem mass spectrometry (MS/MS) using a C18-HPLC column (5 mm, 2.1 × 100 mm), and H2 O-MeCN buffers containing 0.1% formic acid as mobile phase.

Synthesized:

Article Title: High Boron-loaded DNA-Oligomers as Potential Boron Neutron Capture Therapy and Antisense Oligonucleotide Dual-Action Anticancer Agents ‡
Article Snippet: The collected oligonucleotide fractions were desalted on C18 SepPak cartridges (Waters Corp., Milford, MA, USA). .. The molecular mass of all the synthesized oligonucleotides was confirmed by MALDI-TOF mass spectrometry ( ) and their purity by polyacrylamide gel electrophoresis (PAGE) analysis ( ).

Incubation:

Article Title: Identification of putative biomarkers for HIV-associated neurocognitive impairment in the CSF of HIV-infected patients under cART therapy determined by mass spectrometry
Article Snippet: Samples were resuspended in 2 M urea with 20 mM TEABC, incubated in 5 mM dithiothreitol (DTT) at 56°C for 30 min for reduction and 15 mM iodoacetamide (IAA) at room temperature for alkylation, followed by overnight trypsin treatment, with a trypsin-to-protein ratio of 1:20. .. To increase labeling affinity, the samples were acidified with 10 μL of 20 % trifluoroacetic acid (TFA) (Thermo Scientific) followed by cleanup with C18 SepPak cartridges (Waters).

Article Title: Combining LOPIT with differential ultracentrifugation for high-resolution spatial proteomics
Article Snippet: The labelling reaction was quenched by adding 8 μL of 5% (w/v) hydroxylamine (which was prepared in 100 mM HEPES pH 8.5) to every sample followed by a 30-min incubation at room temperature and on a shaker, and a further 100 μL of deionised water followed by a 1-h incubation at 4 °C. .. The combined, TMT-labelled samples were then cleaned using C18 SepPak cartridges (Waters) to remove salts and other substances which could interfere with downstream peptide processing.

Article Title: Cell Surface Proteome of Dental Pulp Stem Cells Identified by Label-Free Mass Spectrometry
Article Snippet: The filters were vortexed and pulse-centrifuged to 2,000 x g , tubes closed and incubated for 4 h to 18 h at 37°C. .. Peptides were desalted using C18-SepPak cartridges (WAT023590, Waters, Milford, MA, USA).

Expressing:

Article Title: Recombinant production of rhesus ?-defensin-1 (RTD-1) using a bacterial expression system
Article Snippet: Paragraph title: In-cell expression of RTD-1 ... Both the soluble cell lysate and solubilized cell lysate pellet were extracted using C18 SepPak cartridges (Waters, Milford, MA) with elution in MeCN:H2 O (3:2 vol.) containing 0.1% TFA and lyophilized.

Article Title: Biosynthesis and antimicrobial evaluation of backbone-cyclized alpha-defensins#
Article Snippet: Paragraph title: In vivo expression of Crp4-1 ... Both the soluble cell lysate and solubilized cell lysate pellet were extracted using C18 SepPak cartridges (Waters, Milford, MA) with elution in MeCN:H2 O (3:2 vol.) containing 0.1% TFA.

BIA-KA:

Article Title: Identification of putative biomarkers for HIV-associated neurocognitive impairment in the CSF of HIV-infected patients under cART therapy determined by mass spectrometry
Article Snippet: Protein amounts were measured using a bicinchoninic acid (BCA) protein assay (Thermo Scientific), and 100 μg were prepared for iTRAQ labeling. .. To increase labeling affinity, the samples were acidified with 10 μL of 20 % trifluoroacetic acid (TFA) (Thermo Scientific) followed by cleanup with C18 SepPak cartridges (Waters).

Modification:

Article Title: High Boron-loaded DNA-Oligomers as Potential Boron Neutron Capture Therapy and Antisense Oligonucleotide Dual-Action Anticancer Agents ‡
Article Snippet: Paragraph title: 3.5. Synthesis and Purification of DNA Oligonucleotides Modified with Boron Cluster ... The collected oligonucleotide fractions were desalted on C18 SepPak cartridges (Waters Corp., Milford, MA, USA).

Transformation Assay:

Article Title: Recombinant production of rhesus ?-defensin-1 (RTD-1) using a bacterial expression system
Article Snippet: Origami2(DE3) cells transformed with the plasmid encoding the RTD-C7 precursor were grown, induced, harvested and lysed as described above. .. Both the soluble cell lysate and solubilized cell lysate pellet were extracted using C18 SepPak cartridges (Waters, Milford, MA) with elution in MeCN:H2 O (3:2 vol.) containing 0.1% TFA and lyophilized.

Article Title: Biosynthesis and antimicrobial evaluation of backbone-cyclized alpha-defensins#
Article Snippet: Origami2(DE3) cells transformed with the plasmid encoding the intein precursor of Crp4-1 were induced, harvested and lysed as described above. .. Both the soluble cell lysate and solubilized cell lysate pellet were extracted using C18 SepPak cartridges (Waters, Milford, MA) with elution in MeCN:H2 O (3:2 vol.) containing 0.1% TFA.

High Performance Liquid Chromatography:

Article Title: Combining LOPIT with differential ultracentrifugation for high-resolution spatial proteomics
Article Snippet: The combined, TMT-labelled samples were then cleaned using C18 SepPak cartridges (Waters) to remove salts and other substances which could interfere with downstream peptide processing. .. In detail, each sample was resuspended in approximately 5 mL of 0.1% (v/v) TFA (Trifluoroacetic acid; prepared in HPLC-grade water) and the pH of the solution adjusted to < 3.

Article Title: Recombinant production of rhesus ?-defensin-1 (RTD-1) using a bacterial expression system
Article Snippet: Both the soluble cell lysate and solubilized cell lysate pellet were extracted using C18 SepPak cartridges (Waters, Milford, MA) with elution in MeCN:H2 O (3:2 vol.) containing 0.1% TFA and lyophilized. .. The samples were dissolved in H2 O containing 0.1% formic acid and analyzed by HPLC-tandem mass spectrometry using a C18-HPLC column (5 mm, 2.1 × 100 mm), and H2 O-MeCN buffers containing 0.1% formic acid as mobile phase.

Article Title: Biosynthesis and antimicrobial evaluation of backbone-cyclized alpha-defensins#
Article Snippet: Both the soluble cell lysate and solubilized cell lysate pellet were extracted using C18 SepPak cartridges (Waters, Milford, MA) with elution in MeCN:H2 O (3:2 vol.) containing 0.1% TFA. .. The samples were analyzed by HPLC-tandem mass spectrometry (MS/MS) using a C18-HPLC column (5 mm, 2.1 × 100 mm), and H2 O-MeCN buffers containing 0.1% formic acid as mobile phase.

Article Title: Proteomic and Functional Investigation of the Colon Cancer Relapse-Associated Genes NOX4 and ITGA3
Article Snippet: The labeled peptides were quenched with 100 μL of HPLC-grade water, dried, and pooled. .. High-pH RP fractionation was performed using 50 mg C18 SepPak cartridges (Waters, Milford, MA) and 10 mmol/L ammonium bicarbonate buffers at pH 10.

Flow Cytometry:

Article Title: High Boron-loaded DNA-Oligomers as Potential Boron Neutron Capture Therapy and Antisense Oligonucleotide Dual-Action Anticancer Agents ‡
Article Snippet: The final solution was briefly degassed with argon and agitated at room temperature for 4 h. Then, the products were isolated by RP-HPLC using a Kinetex 5 µm, C18, 250 × 4.6 mm column with buffer A (0.1 M CH3 COONH4 /H2 O) and buffer B (100% CH3 CN) at a 1 mL/min flow rate. .. The collected oligonucleotide fractions were desalted on C18 SepPak cartridges (Waters Corp., Milford, MA, USA).

Chromatography:

Article Title: Phosphoproteome Analysis Reveals Differential Mode of Action of Sorafenib in Wildtype and Mutated FLT3 Acute Myeloid Leukemia (AML) Cells *
Article Snippet: .. Tryptic peptides were desalted using reversed-phase 500 mg C18 SepPak cartridges (Waters, Eschborn, Germany) and fractionated by SCX chromatography (250 × 9.4 mm polySULFOETHYL A column, 200 Å pore size, 5 μm particle size (PolyLC)) operated with ÄKTA explorer system (GE Healthcare, München, Germany) as described previously ( ). .. The resulting twelve peptide samples were desalted using reversed-phase 100 mg C18 SepPak cartridges (Waters) before phosphopeptide enrichment with immobilized metal affinity chromatography (IMAC) in two successive enrichment steps essentially as described before ( – ).

Protease Inhibitor:

Article Title: Phosphoproteome Analysis Reveals Differential Mode of Action of Sorafenib in Wildtype and Mutated FLT3 Acute Myeloid Leukemia (AML) Cells *
Article Snippet: Cells were lysed in ice-cold lysis buffer (8 M urea, 75 mm NaCl, 50 mm Tris pH 8.2, 5 mm EDTA, 5 mm EGTA, 10 mm sodium pyrophosphate, 10 mm NaF, 10 mm beta-glycerophosphate, 2 mm Na3 VO4, phosphatase inhibitor mixture 1 and 2 (Sigma-Aldrich) and protease inhibitor mixture tablet (Roche Applied Science, Mannheim, Germany)) and sonicated three times for 1 min on ice. .. Tryptic peptides were desalted using reversed-phase 500 mg C18 SepPak cartridges (Waters, Eschborn, Germany) and fractionated by SCX chromatography (250 × 9.4 mm polySULFOETHYL A column, 200 Å pore size, 5 μm particle size (PolyLC)) operated with ÄKTA explorer system (GE Healthcare, München, Germany) as described previously ( ).

Cell Culture:

Article Title: Phosphoproteome Analysis Reveals Differential Mode of Action of Sorafenib in Wildtype and Mutated FLT3 Acute Myeloid Leukemia (AML) Cells *
Article Snippet: Cells were cultured for at least seven cell doublings to reach > 95% of incorporation of amino acids arginine and lysine, before treatment for 2 hours with DMSO, 0.01 or 0.2 μm sorafenib, respectively. .. Tryptic peptides were desalted using reversed-phase 500 mg C18 SepPak cartridges (Waters, Eschborn, Germany) and fractionated by SCX chromatography (250 × 9.4 mm polySULFOETHYL A column, 200 Å pore size, 5 μm particle size (PolyLC)) operated with ÄKTA explorer system (GE Healthcare, München, Germany) as described previously ( ).

other:

Article Title: Telocinobufagin, a Novel Cardiotonic Steroid, Promotes Renal Fibrosis via Na+/K+-ATPase Profibrotic Signaling Pathways
Article Snippet: Plasma samples were extracted for TCB measurements using C18 SepPak cartridges (Waters Inc., Cambridge, MA, USA) and TCB levels were measured using the DELFIA assay, as previously described in detail [ , , ].

Article Title: Design, solid-phase synthesis and evaluation of enterobactin analogs for iron delivery into the human pathogen Campylobacter jejuni
Article Snippet: Following is a list of the sources of other key reagents and expendable materials used in these studies: Polymer-bound 2-chlorotrityl resin, 100-200 Mesh, 1% DVB, 1.4 mmol/g (Novabiochem, 855017); Fmoc-Dap(Alloc)-OH (Bachem (B-2385.0005); 50 mL Torvic solid phase plastic reaction vessels (Torvic); C18 SepPak cartridges (Waters, WAT 023501); BL Mueller-Hinton broth (BD and Co., Cat. No. 211443); ciprofloxacin hydrochloride monohydrate (TCI America, Inc., Cat. No. C2227); ferric chloride (Fisher Scientific, Cat. No. 188-500); MEM-α (Life Technologies, Cat. No. 41061-029); M9 culture media, 5X minimal salts (BD and Co., Cat. No. 248510); Trimethoprim (Chem Impex, Cat. No. 01634); Breathe Easier plate sealing films (Diversified Biotech, Cat. No. BERM-2000); Costar sterile 96-well plates (Corning Incorp., Cat. No. 3595); Oxoid AGS plastic pouches (Invitro Diagnostics, Cat. No., AG0020C); sealing clips for plastic pouches (Invitro Diagnostics, Cat. No. AN0005C); microaerophilic gas mixture for C. jejuni growth (85% N2 , 10% CO2 , 5% O2 ) (AirGas); culture tubes for macroscale bacterial cultures (17 × 100 mm) (VWR, Cat. No. 60818-689).

Tandem Mass Spectroscopy:

Article Title: Biosynthesis and antimicrobial evaluation of backbone-cyclized alpha-defensins#
Article Snippet: Both the soluble cell lysate and solubilized cell lysate pellet were extracted using C18 SepPak cartridges (Waters, Milford, MA) with elution in MeCN:H2 O (3:2 vol.) containing 0.1% TFA. .. The samples were analyzed by HPLC-tandem mass spectrometry (MS/MS) using a C18-HPLC column (5 mm, 2.1 × 100 mm), and H2 O-MeCN buffers containing 0.1% formic acid as mobile phase.

Sonication:

Article Title: Phosphoproteome Analysis Reveals Differential Mode of Action of Sorafenib in Wildtype and Mutated FLT3 Acute Myeloid Leukemia (AML) Cells *
Article Snippet: Cells were lysed in ice-cold lysis buffer (8 M urea, 75 mm NaCl, 50 mm Tris pH 8.2, 5 mm EDTA, 5 mm EGTA, 10 mm sodium pyrophosphate, 10 mm NaF, 10 mm beta-glycerophosphate, 2 mm Na3 VO4, phosphatase inhibitor mixture 1 and 2 (Sigma-Aldrich) and protease inhibitor mixture tablet (Roche Applied Science, Mannheim, Germany)) and sonicated three times for 1 min on ice. .. Tryptic peptides were desalted using reversed-phase 500 mg C18 SepPak cartridges (Waters, Eschborn, Germany) and fractionated by SCX chromatography (250 × 9.4 mm polySULFOETHYL A column, 200 Å pore size, 5 μm particle size (PolyLC)) operated with ÄKTA explorer system (GE Healthcare, München, Germany) as described previously ( ).

Binding Assay:

Article Title: Elevated Plasma Marinobufagenin, An Endogenous Cardiotonic Steroid, Is Associated with Right Ventricular Dysfunction and Nitrative Stress in Heart Failure
Article Snippet: Plasma samples were extracted for MBG measurements using C18 SepPak cartridges (Waters Inc., Cambridge, MA) and MBG levels were measured using a competitive fluoroimmunoassay [dissociation enhanced fluoroimmunoassay (DELFIA)] as previously described , . .. The MBG DELFIA uses a murine monoclonal antibody (anti-MBG 4G4) and employs competition between immobilized antigen (MBG-glycoside-thyroglobulin) and MBG, other cross-reactants, or endogenous cardiotonic steroids within the sample for a limited number of binding sites on the 4G4 anti-MBG monoclonal antibodies.

In Vivo:

Article Title: Biosynthesis and antimicrobial evaluation of backbone-cyclized alpha-defensins#
Article Snippet: Paragraph title: In vivo expression of Crp4-1 ... Both the soluble cell lysate and solubilized cell lysate pellet were extracted using C18 SepPak cartridges (Waters, Milford, MA) with elution in MeCN:H2 O (3:2 vol.) containing 0.1% TFA.

Isolation:

Article Title: High Boron-loaded DNA-Oligomers as Potential Boron Neutron Capture Therapy and Antisense Oligonucleotide Dual-Action Anticancer Agents ‡
Article Snippet: The final solution was briefly degassed with argon and agitated at room temperature for 4 h. Then, the products were isolated by RP-HPLC using a Kinetex 5 µm, C18, 250 × 4.6 mm column with buffer A (0.1 M CH3 COONH4 /H2 O) and buffer B (100% CH3 CN) at a 1 mL/min flow rate. .. The collected oligonucleotide fractions were desalted on C18 SepPak cartridges (Waters Corp., Milford, MA, USA).

Labeling:

Article Title: Identification of putative biomarkers for HIV-associated neurocognitive impairment in the CSF of HIV-infected patients under cART therapy determined by mass spectrometry
Article Snippet: .. To increase labeling affinity, the samples were acidified with 10 μL of 20 % trifluoroacetic acid (TFA) (Thermo Scientific) followed by cleanup with C18 SepPak cartridges (Waters). .. The samples were then dried and stored at −80 °C before iTRAQ labeling.

Article Title: Elevated Plasma Marinobufagenin, An Endogenous Cardiotonic Steroid, Is Associated with Right Ventricular Dysfunction and Nitrative Stress in Heart Failure
Article Snippet: Plasma samples were extracted for MBG measurements using C18 SepPak cartridges (Waters Inc., Cambridge, MA) and MBG levels were measured using a competitive fluoroimmunoassay [dissociation enhanced fluoroimmunoassay (DELFIA)] as previously described , . .. Secondary (goat anti-mouse) europium labeled antibody was obtained from Perkin-Elmer (Waltham, MA).

Article Title: Proteomic and Functional Investigation of the Colon Cancer Relapse-Associated Genes NOX4 and ITGA3
Article Snippet: Excess labeling reagents were removed from the pooled peptide samples with a SCX SpinTip sample preparation kit (Protea Biosciences, Morgantown, WV) following the manufacturer’s instructions. .. High-pH RP fractionation was performed using 50 mg C18 SepPak cartridges (Waters, Milford, MA) and 10 mmol/L ammonium bicarbonate buffers at pH 10.

Purification:

Article Title: High Boron-loaded DNA-Oligomers as Potential Boron Neutron Capture Therapy and Antisense Oligonucleotide Dual-Action Anticancer Agents ‡
Article Snippet: Paragraph title: 3.5. Synthesis and Purification of DNA Oligonucleotides Modified with Boron Cluster ... The collected oligonucleotide fractions were desalted on C18 SepPak cartridges (Waters Corp., Milford, MA, USA).

Article Title: Molecular basis for intestinal mucin recognition by galectin-3 and C-type lectins
Article Snippet: .. The permethylated N -glycans were then purified on C18-SepPak cartridges. ..

Polyacrylamide Gel Electrophoresis:

Article Title: High Boron-loaded DNA-Oligomers as Potential Boron Neutron Capture Therapy and Antisense Oligonucleotide Dual-Action Anticancer Agents ‡
Article Snippet: The collected oligonucleotide fractions were desalted on C18 SepPak cartridges (Waters Corp., Milford, MA, USA). .. The molecular mass of all the synthesized oligonucleotides was confirmed by MALDI-TOF mass spectrometry ( ) and their purity by polyacrylamide gel electrophoresis (PAGE) analysis ( ).

Lysis:

Article Title: Phosphoproteome Analysis Reveals Differential Mode of Action of Sorafenib in Wildtype and Mutated FLT3 Acute Myeloid Leukemia (AML) Cells *
Article Snippet: Cells were lysed in ice-cold lysis buffer (8 M urea, 75 mm NaCl, 50 mm Tris pH 8.2, 5 mm EDTA, 5 mm EGTA, 10 mm sodium pyrophosphate, 10 mm NaF, 10 mm beta-glycerophosphate, 2 mm Na3 VO4, phosphatase inhibitor mixture 1 and 2 (Sigma-Aldrich) and protease inhibitor mixture tablet (Roche Applied Science, Mannheim, Germany)) and sonicated three times for 1 min on ice. .. Tryptic peptides were desalted using reversed-phase 500 mg C18 SepPak cartridges (Waters, Eschborn, Germany) and fractionated by SCX chromatography (250 × 9.4 mm polySULFOETHYL A column, 200 Å pore size, 5 μm particle size (PolyLC)) operated with ÄKTA explorer system (GE Healthcare, München, Germany) as described previously ( ).

Plasmid Preparation:

Article Title: Recombinant production of rhesus ?-defensin-1 (RTD-1) using a bacterial expression system
Article Snippet: Origami2(DE3) cells transformed with the plasmid encoding the RTD-C7 precursor were grown, induced, harvested and lysed as described above. .. Both the soluble cell lysate and solubilized cell lysate pellet were extracted using C18 SepPak cartridges (Waters, Milford, MA) with elution in MeCN:H2 O (3:2 vol.) containing 0.1% TFA and lyophilized.

Article Title: Biosynthesis and antimicrobial evaluation of backbone-cyclized alpha-defensins#
Article Snippet: Origami2(DE3) cells transformed with the plasmid encoding the intein precursor of Crp4-1 were induced, harvested and lysed as described above. .. Both the soluble cell lysate and solubilized cell lysate pellet were extracted using C18 SepPak cartridges (Waters, Milford, MA) with elution in MeCN:H2 O (3:2 vol.) containing 0.1% TFA.

Affinity Chromatography:

Article Title: Phosphoproteome Analysis Reveals Differential Mode of Action of Sorafenib in Wildtype and Mutated FLT3 Acute Myeloid Leukemia (AML) Cells *
Article Snippet: Tryptic peptides were desalted using reversed-phase 500 mg C18 SepPak cartridges (Waters, Eschborn, Germany) and fractionated by SCX chromatography (250 × 9.4 mm polySULFOETHYL A column, 200 Å pore size, 5 μm particle size (PolyLC)) operated with ÄKTA explorer system (GE Healthcare, München, Germany) as described previously ( ). .. The resulting twelve peptide samples were desalted using reversed-phase 100 mg C18 SepPak cartridges (Waters) before phosphopeptide enrichment with immobilized metal affinity chromatography (IMAC) in two successive enrichment steps essentially as described before ( – ).

Sample Prep:

Article Title: Identification of putative biomarkers for HIV-associated neurocognitive impairment in the CSF of HIV-infected patients under cART therapy determined by mass spectrometry
Article Snippet: Paragraph title: Sample preparation ... To increase labeling affinity, the samples were acidified with 10 μL of 20 % trifluoroacetic acid (TFA) (Thermo Scientific) followed by cleanup with C18 SepPak cartridges (Waters).

Article Title: Proteomic and Functional Investigation of the Colon Cancer Relapse-Associated Genes NOX4 and ITGA3
Article Snippet: Excess labeling reagents were removed from the pooled peptide samples with a SCX SpinTip sample preparation kit (Protea Biosciences, Morgantown, WV) following the manufacturer’s instructions. .. High-pH RP fractionation was performed using 50 mg C18 SepPak cartridges (Waters, Milford, MA) and 10 mmol/L ammonium bicarbonate buffers at pH 10.

Fractionation:

Article Title: Combining LOPIT with differential ultracentrifugation for high-resolution spatial proteomics
Article Snippet: Paragraph title: HyperLOPIT subcellular fractionation ... The combined, TMT-labelled samples were then cleaned using C18 SepPak cartridges (Waters) to remove salts and other substances which could interfere with downstream peptide processing.

Article Title: Proteomic and Functional Investigation of the Colon Cancer Relapse-Associated Genes NOX4 and ITGA3
Article Snippet: .. High-pH RP fractionation was performed using 50 mg C18 SepPak cartridges (Waters, Milford, MA) and 10 mmol/L ammonium bicarbonate buffers at pH 10. ..

Reversed-phase Chromatography:

Article Title: Combining LOPIT with differential ultracentrifugation for high-resolution spatial proteomics
Article Snippet: .. After labelling, peptides were pooled into 10-plexes, cleaned with C18 SepPak cartridges and fractionated using high-pH reverse phase chromatography as described above for our hyperLOPIT experiments. .. The resulting fractions corresponding to each TMT 10-plex set were orthogonally combined into 18 samples for downstream MS analysis.

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94
    Waters Corporation cartridge
    Cartridge, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cartridge/product/Waters Corporation
    Average 94 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    cartridge - by Bioz Stars, 2020-04
    94/100 stars
      Buy from Supplier

    98
    Waters Corporation solid phase extraction
    Solid Phase Extraction, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 98/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/solid phase extraction/product/Waters Corporation
    Average 98 stars, based on 38 article reviews
    Price from $9.99 to $1999.99
    solid phase extraction - by Bioz Stars, 2020-04
    98/100 stars
      Buy from Supplier

    87
    Waters Corporation tc18 light reverse phase silica cartridge
    Tc18 Light Reverse Phase Silica Cartridge, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 87/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tc18 light reverse phase silica cartridge/product/Waters Corporation
    Average 87 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tc18 light reverse phase silica cartridge - by Bioz Stars, 2020-04
    87/100 stars
      Buy from Supplier

    86
    Waters Corporation pre conditioned seppak c18 cartridge
    Pre Conditioned Seppak C18 Cartridge, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pre conditioned seppak c18 cartridge/product/Waters Corporation
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pre conditioned seppak c18 cartridge - by Bioz Stars, 2020-04
    86/100 stars
      Buy from Supplier

    Image Search Results