c18 cleanup columns  (Thermo Fisher)


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    Name:
    Pierce C18 Spin Columns
    Description:
    Thermo Scientific Pierce C18 Spin Columns are ready to use centrifuge columns of porous C18 reverse phase resin with excellent binding and recovery characteristics for peptide sample preparation for mass spectrometry and other methods Peptide samples can be purified and concentrated for a variety of applications using Pierce C18 Spin Columns Each spin column contains a porous C18 reversed phase resin with excellent binding and recovery characteristics for a wide range of peptide concentrations The spin column format allows simultaneous processing of multiple samples 10 to 150 µL each in approximately 30 minutes without laborious repeat pipetting or specialized equipment Pierce C18 Spin Columns can be used effectively for processing peptides derived from 10 ng to 30 µg of protein Sensitivity and detection limits are dependent on the downstream application Features of C18 Spin Columns • Removes interfering contaminants significantly reduces signal suppression and improves signal to noise ratios and sequence coverage • Simplifies optimization processing yields high quality spectra and is effective for a variety of reverse phase compatible contaminates • Robust works with a wide variety of load volumes and concentrations no need to reduce sample volume before application • Convenient easy to handle and requires no special equipment to process multiple samples simultaneously unlike tip driven systems that require one sample to be processed at a time • Sensitive special C18 resin allows excellent recovery percentages even at low sub picomole sample loads Matrix assisted laser desorption ionization MALDI and electrospray ionization ESI mass spectrometry MS are essential for studying biological compounds because of the high sensitivity and mass accuracy they provide However many of the buffers and compounds common to biological samples interfere with both MALDI MS and ESI MS resulting in reduced sensitivity and poor quality spectra Pierce C 18 Spin Columns remove interfering contaminants and release peptides in MS compatible solutions providing an effective tool that aids in the MS examination of post translational modifications or for the identification of proteins by peptide mapping
    Catalog Number:
    89870
    Price:
    None
    Applications:
    Protein Biology|Protein Mass Spectrometry Analysis|Sample Prep for Mass Spectrometry
    Category:
    Kits and Assays
    Buy from Supplier


    Structured Review

    Thermo Fisher c18 cleanup columns
    Thermo Scientific Pierce C18 Spin Columns are ready to use centrifuge columns of porous C18 reverse phase resin with excellent binding and recovery characteristics for peptide sample preparation for mass spectrometry and other methods Peptide samples can be purified and concentrated for a variety of applications using Pierce C18 Spin Columns Each spin column contains a porous C18 reversed phase resin with excellent binding and recovery characteristics for a wide range of peptide concentrations The spin column format allows simultaneous processing of multiple samples 10 to 150 µL each in approximately 30 minutes without laborious repeat pipetting or specialized equipment Pierce C18 Spin Columns can be used effectively for processing peptides derived from 10 ng to 30 µg of protein Sensitivity and detection limits are dependent on the downstream application Features of C18 Spin Columns • Removes interfering contaminants significantly reduces signal suppression and improves signal to noise ratios and sequence coverage • Simplifies optimization processing yields high quality spectra and is effective for a variety of reverse phase compatible contaminates • Robust works with a wide variety of load volumes and concentrations no need to reduce sample volume before application • Convenient easy to handle and requires no special equipment to process multiple samples simultaneously unlike tip driven systems that require one sample to be processed at a time • Sensitive special C18 resin allows excellent recovery percentages even at low sub picomole sample loads Matrix assisted laser desorption ionization MALDI and electrospray ionization ESI mass spectrometry MS are essential for studying biological compounds because of the high sensitivity and mass accuracy they provide However many of the buffers and compounds common to biological samples interfere with both MALDI MS and ESI MS resulting in reduced sensitivity and poor quality spectra Pierce C 18 Spin Columns remove interfering contaminants and release peptides in MS compatible solutions providing an effective tool that aids in the MS examination of post translational modifications or for the identification of proteins by peptide mapping
    https://www.bioz.com/result/c18 cleanup columns/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    c18 cleanup columns - by Bioz Stars, 2020-09
    99/100 stars

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    other:

    Article Title: Clinical and Pharmacological Investigation of Myotoxicity in Sri Lankan Russell’s Viper (Daboia russelii) Envenoming
    Article Snippet: The resulting peptides were desalted with Pierce C18 Spin columns (ThermoFisher Scientific), dried, resuspended in 2% Acetronitrile, 0.1% TFA and analysed by LC-MS/MS analysis as previously described [ ].

    Article Title: Global Proteomic and Methylome Analysis in Human Induced Pluripotent Stem Cells Reveals Overexpression of a Human TLR3 Affecting Proper Innate Immune Response Signaling
    Article Snippet: Mixture of samples was cleaned by Pierce C18 Spin columns (Thermo Scientific, Rockford, IL) and stored at −80°C.

    Article Title: The N-cadherin interactome in primary cardiomyocytes as defined using quantitative proximity proteomics
    Article Snippet: The peptides were extracted from gel pieces in a solution containing 70% ACN, 5% formic acid and desalted with Pierce C18 Spin Columns (Thermo Fisher Scientific) according to manufacturer's protocol, dried in a vacuum centrifuge and resuspended in 18 µl of 0.1% formic acid.

    Article Title: Interplay between Gliotoxin Resistance, Secretion, and the Methyl/Methionine Cycle in Aspergillus fumigatus
    Article Snippet: Digested samples were desalted prior to analysis by using either C18 spin columns (Thermo Scientific Pierce) or C18 ZipTips (Millipore).

    Article Title: Single-Stranded Nucleic Acids Bind to the Tetramer Interface of SAMHD1 and Prevent Formation of the Catalytic Homotetramer
    Article Snippet: The dried nuclease digestion was desalted using C18 cleanup columns containing 8 mg of C-18 resin (Pierce), according the manufacturer instructions.

    Article Title: Proteomic identification of membrane-associated placental protein 4 (MP4) as perlecan and characterization of its placental expression in normal and pathologic pregnancies
    Article Snippet: Cleaning of digested samples Digested MP4 and placental tissue samples were cleaned using Pierce™ C18 Spin Columns (Thermo Fisher Scientific, Waltham, MA, USA) following the manufacturer’s protocol.

    Article Title: Cyclodextrins: Assessing the Impact of Cavity Size, Occupancy, and Substitutions on Cytotoxicity and Cholesterol Homeostasis
    Article Snippet: Two micrograms of trypsin (Promega) were added for overnight digestion (18 h), and then the tryptic peptides were desalted using Pierce C18 spin columns (Thermo Fischer Scientific).

    BIA-KA:

    Article Title: Optimization and comparison of bottom-up proteomic sample preparation for early stage Xenopus laevis embryos
    Article Snippet: .. Acetonitrile (ACN), formic acid (FA), Pierce C-18 Spin Columns, and Pierce BCA Protein Assays were purchased from Thermo Scientific (Marietta, OH USA). .. Sep-Pak Vac (1 cc, 100 mg) C-18 Cartridges were purchased from Waters (Ireland).

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