c18 acclaim pepmap100  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    Thermo Fisher c18 acclaim pepmap100
    C18 Acclaim Pepmap100, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 57 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/c18 acclaim pepmap100/product/Thermo Fisher
    Average 99 stars, based on 57 article reviews
    Price from $9.99 to $1999.99
    c18 acclaim pepmap100 - by Bioz Stars, 2020-04
    99/100 stars

    Images

    Related Articles

    High Performance Liquid Chromatography:

    Article Title: ENHANCED SENSITIVITY OF LC-MS ANALYSIS OF PERMETHYLATED N-GLYCANS THROUGH ONLINE PURIFICATION
    Article Snippet: Next, samples were initially injected onto an Acclaim® PepMap100 C18 nano-trap column (Dionex, Sunnyvale, CA). .. The samples were then washed using mobile phase A, consisting of 98% HPLC grade water, 2% ACN, and 0.1% formic acid.

    Article Title: Discrimination of grade 2 and 3 cervical intraepithelial neoplasia by means of analysis of water soluble proteins recovered from cervical biopsies
    Article Snippet: LC-MS/MS analysis A Dionex Ultimate 3000 nanoflow HPLC equipped with a 300 μm i.d × 0.5 cm length Acclaim PepMap 100 C18 trap column and a 75 μm id. .. × 15 cm Acclaim PepMap 100 C18 analytical column (Dionex) were used with a LTQ-Orbitrap hybrid mass spectrometer (Thermo Scientific).

    Article Title: The Interplay of Light and Oxygen in the Reactive Oxygen Stress Response of Chlamydomonas reinhardtii Dissected by Quantitative Mass Spectrometry *
    Article Snippet: Reversed phase (2 h gradients) of peptide samples via HPLC was performed using a Dionex Ultimate 3000 Nano LC System (Thermo Scientific). .. For peptide separation, the trap column was switched in-line with a C18 capillary column (Acclaim PepMap 100, 75 μm × 150 mm, 3 μm particle size, 100 Å pore size, Dionex) and the following gradient was applied: 0–35% B (85 min), 35–50% B (10 min), 50–100% B (5 min), 100% B (5 min).

    Flow Cytometry:

    Article Title: Homeolog expression analysis in an allotriploid non-model crop via integration of transcriptomics and proteomics
    Article Snippet: .. The digested samples (1 µg/5 µL) were injected and separated on an Ultimate 3000 UPLC system (Dionex, Thermo Scientific, USA) equipped with a Acclaim PepMap100 pre column (C18 3 µm-100 Å, Thermo Scientific, USA) and an C18 PepMap RSLC (2 µm, 50 × 50 cm, Thermo Scientific, USA) following a multiple step gradient with a linear gradient (flow rate: 0.300 µL/min): 4% buffer B (80% acetonitrile (CAN), in 0.08% formic acid (FA)) for 3 min, 4–12% in 17 min, 12–15% in 10 min, 15–18% in 45 min, 18–20% in 40 min, 20–30% in 65 min, 30–35% in 30 min, 35–65% in 10 min, 65–95% in 5 min, 95% for 40 min, 95–4% in 1 min, 4% for 39 min. .. The Q Exactive Orbitrap mass spectrometer (Thermo Scientific, USA) was operated in positive ion mode with a nano spray voltage of 1.5 kV and the source temperature set to 250 °C.

    Article Title: Potential role of new molecular plasma signatures on cardiovascular risk stratification in asymptomatic individuals
    Article Snippet: Digested samples (1 µg of protein and 25 fmol of each heavy-labeled peptide) were injected onto an Acclaim PepMap100 C18 trap column (100 um × 2 cm, Thermo scientific) and then separated by RP-HPLC on an Acclaim PepMap RSLC C18 column (75 um × 15 cm, Thermo scientific). .. Chromatography was performed with solvent A (0.1% FA) and solvent B (100% ACN, 0.1% FA) as a mobile phase, using at a 300 nl/min flow rate and a linear gradient: 60 minutes from 2% B to 40% B; 5 minutes to 50% B; and 5 minutes at 90% B before returning to 2% B).

    Article Title: ENHANCED SENSITIVITY OF LC-MS ANALYSIS OF PERMETHYLATED N-GLYCANS THROUGH ONLINE PURIFICATION
    Article Snippet: Next, samples were initially injected onto an Acclaim® PepMap100 C18 nano-trap column (Dionex, Sunnyvale, CA). .. The washing was performed for 10 min at a flow rate of 3 μl/min. prior to loading to a C18 column.

    Article Title: Proteomic analysis of rat serum revealed the effects of chronic sleep deprivation on metabolic, cardiovascular and nervous system
    Article Snippet: The eluted peptides were separated on a pre-column packed with C18 Luna beads (3 μm diameter, 100 Å pore size; Thermo Fisher Scientific 164946) coupled to an analytical column packed with C18 Luna beads (3 μm diameter, 100 Å pore size; Thermo Fisher Scientific 164568). .. Subsequently, the peptides were eluted with a linear gradient from 4% B to 35% B in 90 min with a constant flow rate of 400 nl/min.

    Article Title: Discrimination of grade 2 and 3 cervical intraepithelial neoplasia by means of analysis of water soluble proteins recovered from cervical biopsies
    Article Snippet: × 15 cm Acclaim PepMap 100 C18 analytical column (Dionex) were used with a LTQ-Orbitrap hybrid mass spectrometer (Thermo Scientific). .. 5 μl of the tryptic digests were injected onto the trap column using 0.1% formic acid (FA, VWR) in MilliQ-water (Elga) at a flow of 2 μl/min.

    Article Title: The Interplay of Light and Oxygen in the Reactive Oxygen Stress Response of Chlamydomonas reinhardtii Dissected by Quantitative Mass Spectrometry *
    Article Snippet: Samples were loaded on a trap column (Acclaim PepMap100, 300 μm × 5 mm, 5 μm particle size, 100 Å pore size; Dionex) and desalted at a flow rate of 25 μl/min for 4 min using eluent A. .. For peptide separation, the trap column was switched in-line with a C18 capillary column (Acclaim PepMap 100, 75 μm × 150 mm, 3 μm particle size, 100 Å pore size, Dionex) and the following gradient was applied: 0–35% B (85 min), 35–50% B (10 min), 50–100% B (5 min), 100% B (5 min).

    Mass Spectrometry:

    Article Title: Homeolog expression analysis in an allotriploid non-model crop via integration of transcriptomics and proteomics
    Article Snippet: Paragraph title: Protein extraction and data acquisition through mass spectrometry ... The digested samples (1 µg/5 µL) were injected and separated on an Ultimate 3000 UPLC system (Dionex, Thermo Scientific, USA) equipped with a Acclaim PepMap100 pre column (C18 3 µm-100 Å, Thermo Scientific, USA) and an C18 PepMap RSLC (2 µm, 50 × 50 cm, Thermo Scientific, USA) following a multiple step gradient with a linear gradient (flow rate: 0.300 µL/min): 4% buffer B (80% acetonitrile (CAN), in 0.08% formic acid (FA)) for 3 min, 4–12% in 17 min, 12–15% in 10 min, 15–18% in 45 min, 18–20% in 40 min, 20–30% in 65 min, 30–35% in 30 min, 35–65% in 10 min, 65–95% in 5 min, 95% for 40 min, 95–4% in 1 min, 4% for 39 min.

    Article Title: Potential role of new molecular plasma signatures on cardiovascular risk stratification in asymptomatic individuals
    Article Snippet: SRM-MS SRM experiments were performed on a 5500 QTRAP hybrid triple quadrupole/linear ion trap mass spectrometer (ABSciex) equipped with an Eksigent 1D + plus nanoLC chromatographic system. .. Digested samples (1 µg of protein and 25 fmol of each heavy-labeled peptide) were injected onto an Acclaim PepMap100 C18 trap column (100 um × 2 cm, Thermo scientific) and then separated by RP-HPLC on an Acclaim PepMap RSLC C18 column (75 um × 15 cm, Thermo scientific).

    Article Title: Mass spectrometry based analysis of human plasma-derived factor X revealed novel post-translational modifications
    Article Snippet: .. Peptides obtained after digestion were injected on an Acclaim PepMap100 C18 column (0.075 × 150 mm, 3 µm) using a U3000 nano - LC system fitted with a preconcentration setup and interfaced with a high-resolution LTQ-Orbitrap Discovery mass spectrometer (Thermofischer Scientific, Bremen, Germany) operating in the positive ion mode. ..

    Article Title: The ovine hepatic mitochondrial proteome: Understanding seasonal weight loss tolerance in two distinct breeds
    Article Snippet: Protein identification using LC-MS/MS Peptide samples were next analyzed by nanoLC-MS/MS using an Ultimate 3000 liquid chromatography system coupled to a Q-Exactive Hybrid Quadrupole-Orbitrap mass spectrometer (Thermo Scientific, Bremen, Germany) as described [ ]. .. Samples were loaded onto a trapping cartridge (Acclaim PepMap C18 100A°, 5 mm x 300 μm i.d., 160454, Thermo Scientific) in a mobile phase of 2% ACN, 0.1% FA at 10 μL/min.

    Article Title: Distinct proteome pathology of circulating microparticles in systemic lupus erythematosus
    Article Snippet: Paragraph title: Nano-LC-tandem mass spectrometry ... LC–MS/MS was performed by loading 5 μL of the peptides at 200 nL/min on an Acclaim PepMap100 C18 precolumn (100 μm × 2 cm, 5 μm particle size, 100 Å, Thermo Fischer Scientific) in line with an EASY-spray PepMap100 C18 analytical column with integrated emitter (75 μm inner diameter, 150 mm long, 3 μm particle size, 100 Å, Thermo Fisher Scientific).

    Article Title: Proteomic analysis of rat serum revealed the effects of chronic sleep deprivation on metabolic, cardiovascular and nervous system
    Article Snippet: The peptides for DDA and PRM analysis were analyzed by nanoflow liquid chromatography-tandem mass spectrometry using a Q-Exactive plus mass spectrometer (Thermo, USA). .. The eluted peptides were separated on a pre-column packed with C18 Luna beads (3 μm diameter, 100 Å pore size; Thermo Fisher Scientific 164946) coupled to an analytical column packed with C18 Luna beads (3 μm diameter, 100 Å pore size; Thermo Fisher Scientific 164568).

    Article Title: Discrimination of grade 2 and 3 cervical intraepithelial neoplasia by means of analysis of water soluble proteins recovered from cervical biopsies
    Article Snippet: .. × 15 cm Acclaim PepMap 100 C18 analytical column (Dionex) were used with a LTQ-Orbitrap hybrid mass spectrometer (Thermo Scientific). .. 5 μl of the tryptic digests were injected onto the trap column using 0.1% formic acid (FA, VWR) in MilliQ-water (Elga) at a flow of 2 μl/min.

    Article Title: Identification of the phosphorylation sites in the survival motor neuron protein by protein kinase A
    Article Snippet: Paragraph title: 2.5. Mass spectrometry (MS) analysis ... Each sample was then separated on a Dionex 3000 nLC system (Sunnyvale, CA) equipped with an Acclaim PepMap 100 C18 trap column (300 μm × 5 mm, 5 μm, Dionex) and an Acclaim PepMap 100 C18 analytical column (75 μm × 15 cm, 3 μm, Dionex).

    Article Title: The Interplay of Light and Oxygen in the Reactive Oxygen Stress Response of Chlamydomonas reinhardtii Dissected by Quantitative Mass Spectrometry *
    Article Snippet: Liquid chromatography coupled tandem mass spectrometry (LC-MS/MS) measurements were performed using a LTQ Orbitrap XL (Thermo Scientific, Bremen, Germany). .. For peptide separation, the trap column was switched in-line with a C18 capillary column (Acclaim PepMap 100, 75 μm × 150 mm, 3 μm particle size, 100 Å pore size, Dionex) and the following gradient was applied: 0–35% B (85 min), 35–50% B (10 min), 50–100% B (5 min), 100% B (5 min).

    Article Title: Not just fat: investigating the proteome of cetacean blubber tissue
    Article Snippet: These were analysed using nanoflow Liquid Chromatography Electrospray Ionization in tandem with Mass Spectrometry (nLC-ESI MS/MS) of in-gel trypsin digests. .. The peptides were then injected on an Acclaim PepMap 100 C18 trap and an Acclaim PepMap RSLC C18 column (ThermoFisher Scientific) using a nanoLC Ultra 2D plus loading pump and nanoLC as-2 autosampler (Eksigent).

    Chromatography:

    Article Title: Potential role of new molecular plasma signatures on cardiovascular risk stratification in asymptomatic individuals
    Article Snippet: Digested samples (1 µg of protein and 25 fmol of each heavy-labeled peptide) were injected onto an Acclaim PepMap100 C18 trap column (100 um × 2 cm, Thermo scientific) and then separated by RP-HPLC on an Acclaim PepMap RSLC C18 column (75 um × 15 cm, Thermo scientific). .. Chromatography was performed with solvent A (0.1% FA) and solvent B (100% ACN, 0.1% FA) as a mobile phase, using at a 300 nl/min flow rate and a linear gradient: 60 minutes from 2% B to 40% B; 5 minutes to 50% B; and 5 minutes at 90% B before returning to 2% B).

    Article Title: Proteomic analysis of rat serum revealed the effects of chronic sleep deprivation on metabolic, cardiovascular and nervous system
    Article Snippet: The peptides for DDA and PRM analysis were analyzed by nanoflow liquid chromatography-tandem mass spectrometry using a Q-Exactive plus mass spectrometer (Thermo, USA). .. The eluted peptides were separated on a pre-column packed with C18 Luna beads (3 μm diameter, 100 Å pore size; Thermo Fisher Scientific 164946) coupled to an analytical column packed with C18 Luna beads (3 μm diameter, 100 Å pore size; Thermo Fisher Scientific 164568).

    Liquid Chromatography:

    Article Title: The ovine hepatic mitochondrial proteome: Understanding seasonal weight loss tolerance in two distinct breeds
    Article Snippet: Protein identification using LC-MS/MS Peptide samples were next analyzed by nanoLC-MS/MS using an Ultimate 3000 liquid chromatography system coupled to a Q-Exactive Hybrid Quadrupole-Orbitrap mass spectrometer (Thermo Scientific, Bremen, Germany) as described [ ]. .. Samples were loaded onto a trapping cartridge (Acclaim PepMap C18 100A°, 5 mm x 300 μm i.d., 160454, Thermo Scientific) in a mobile phase of 2% ACN, 0.1% FA at 10 μL/min.

    Article Title: The Interplay of Light and Oxygen in the Reactive Oxygen Stress Response of Chlamydomonas reinhardtii Dissected by Quantitative Mass Spectrometry *
    Article Snippet: Liquid chromatography coupled tandem mass spectrometry (LC-MS/MS) measurements were performed using a LTQ Orbitrap XL (Thermo Scientific, Bremen, Germany). .. For peptide separation, the trap column was switched in-line with a C18 capillary column (Acclaim PepMap 100, 75 μm × 150 mm, 3 μm particle size, 100 Å pore size, Dionex) and the following gradient was applied: 0–35% B (85 min), 35–50% B (10 min), 50–100% B (5 min), 100% B (5 min).

    Article Title: Not just fat: investigating the proteome of cetacean blubber tissue
    Article Snippet: These were analysed using nanoflow Liquid Chromatography Electrospray Ionization in tandem with Mass Spectrometry (nLC-ESI MS/MS) of in-gel trypsin digests. .. The peptides were then injected on an Acclaim PepMap 100 C18 trap and an Acclaim PepMap RSLC C18 column (ThermoFisher Scientific) using a nanoLC Ultra 2D plus loading pump and nanoLC as-2 autosampler (Eksigent).

    Distillation:

    Article Title: Porous polymer monolithic column with surface-bound gold nanoparticles for the capture and separation of cysteine-containing peptides
    Article Snippet: EDMA and GMA were purified by passing them through an inhibitor remover column (Sigma-Aldrich, St. Louis, MO, USA) followed by distillation in vacuum. .. An Acclaim PepMap™ C18 Nanocolumn (3 μm particle size, 100 Å pore size, 15 cm × 75 μm I.D.), a product of Dionex Corp. (Sunnyvale, CA, USA) was used in this study.

    Incubation:

    Article Title: Mass spectrometry based analysis of human plasma-derived factor X revealed novel post-translational modifications
    Article Snippet: After cooling at room temperature, 10 μL of a 200 m M IAM solution in water were added and the solution was incubated at RT for 30 min in the dark. .. Peptides obtained after digestion were injected on an Acclaim PepMap100 C18 column (0.075 × 150 mm, 3 µm) using a U3000 nano - LC system fitted with a preconcentration setup and interfaced with a high-resolution LTQ-Orbitrap Discovery mass spectrometer (Thermofischer Scientific, Bremen, Germany) operating in the positive ion mode.

    Protein Extraction:

    Article Title: Homeolog expression analysis in an allotriploid non-model crop via integration of transcriptomics and proteomics
    Article Snippet: Paragraph title: Protein extraction and data acquisition through mass spectrometry ... The digested samples (1 µg/5 µL) were injected and separated on an Ultimate 3000 UPLC system (Dionex, Thermo Scientific, USA) equipped with a Acclaim PepMap100 pre column (C18 3 µm-100 Å, Thermo Scientific, USA) and an C18 PepMap RSLC (2 µm, 50 × 50 cm, Thermo Scientific, USA) following a multiple step gradient with a linear gradient (flow rate: 0.300 µL/min): 4% buffer B (80% acetonitrile (CAN), in 0.08% formic acid (FA)) for 3 min, 4–12% in 17 min, 12–15% in 10 min, 15–18% in 45 min, 18–20% in 40 min, 20–30% in 65 min, 30–35% in 30 min, 35–65% in 10 min, 65–95% in 5 min, 95% for 40 min, 95–4% in 1 min, 4% for 39 min.

    Purification:

    Article Title: Porous polymer monolithic column with surface-bound gold nanoparticles for the capture and separation of cysteine-containing peptides
    Article Snippet: EDMA and GMA were purified by passing them through an inhibitor remover column (Sigma-Aldrich, St. Louis, MO, USA) followed by distillation in vacuum. .. An Acclaim PepMap™ C18 Nanocolumn (3 μm particle size, 100 Å pore size, 15 cm × 75 μm I.D.), a product of Dionex Corp. (Sunnyvale, CA, USA) was used in this study.

    Article Title: ENHANCED SENSITIVITY OF LC-MS ANALYSIS OF PERMETHYLATED N-GLYCANS THROUGH ONLINE PURIFICATION
    Article Snippet: Paragraph title: On-line purification of permethylated N-glycans and LC-MS and LC-MSMS ... Next, samples were initially injected onto an Acclaim® PepMap100 C18 nano-trap column (Dionex, Sunnyvale, CA).

    Concentration Assay:

    Article Title: ENHANCED SENSITIVITY OF LC-MS ANALYSIS OF PERMETHYLATED N-GLYCANS THROUGH ONLINE PURIFICATION
    Article Snippet: Accordingly, samples are resuspended in a 2% aqueous ACN solution containing 0.1% FA solution to make 20% aqueous DMSO/ACN solution with final glycoprotein concentration equivalent to 1μg/μl. .. Next, samples were initially injected onto an Acclaim® PepMap100 C18 nano-trap column (Dionex, Sunnyvale, CA).

    Article Title: Distinct proteome pathology of circulating microparticles in systemic lupus erythematosus
    Article Snippet: Nano-LC-tandem mass spectrometry The digested samples were thawed, mixed on a whirlmixer, acidified (5% (v/v) formic acid, final concentration), and centrifuged (12,000×g /2 min) to remove insoluble material. .. LC–MS/MS was performed by loading 5 μL of the peptides at 200 nL/min on an Acclaim PepMap100 C18 precolumn (100 μm × 2 cm, 5 μm particle size, 100 Å, Thermo Fischer Scientific) in line with an EASY-spray PepMap100 C18 analytical column with integrated emitter (75 μm inner diameter, 150 mm long, 3 μm particle size, 100 Å, Thermo Fisher Scientific).

    Generated:

    Article Title: The ovine hepatic mitochondrial proteome: Understanding seasonal weight loss tolerance in two distinct breeds
    Article Snippet: Samples were loaded onto a trapping cartridge (Acclaim PepMap C18 100A°, 5 mm x 300 μm i.d., 160454, Thermo Scientific) in a mobile phase of 2% ACN, 0.1% FA at 10 μL/min. .. Separation was generated by mixing A: 0.1% FA, and B: 80% ACN, with the following gradient: 22 min (2.5% B to 50% B), 5 min (50% B to 95% B), 10 min (hold 95% B).

    Article Title: The Interplay of Light and Oxygen in the Reactive Oxygen Stress Response of Chlamydomonas reinhardtii Dissected by Quantitative Mass Spectrometry *
    Article Snippet: For peptide separation, the trap column was switched in-line with a C18 capillary column (Acclaim PepMap 100, 75 μm × 150 mm, 3 μm particle size, 100 Å pore size, Dionex) and the following gradient was applied: 0–35% B (85 min), 35–50% B (10 min), 50–100% B (5 min), 100% B (5 min). .. Finally, ions were generated by electrospray ionization (ESI) and full MS1 were measured in the Orbitrap, i.e. , a Fourier transformation mass spectrometer (mass range: 375–1800 m / z ; resolution: 60,000).

    Article Title: Not just fat: investigating the proteome of cetacean blubber tissue
    Article Snippet: The peptides were then injected on an Acclaim PepMap 100 C18 trap and an Acclaim PepMap RSLC C18 column (ThermoFisher Scientific) using a nanoLC Ultra 2D plus loading pump and nanoLC as-2 autosampler (Eksigent). .. The eluate was sprayed into a TripleTOF 5600+ electrospray tandem mass spectrometer (Sciex, Foster City, CA) and analysed in Information Dependent Acquisition (IDA) mode, performing 250 ms of MS followed by 100 ms MSMS analyses on the 20 most intense peaks seen by MS The MS/MS data file generated via the ‘Create mgf file’ script in PeakView (Sciex) was analysed using the Mascot search algorithm (Matrix Science), against the NCBInr database (Apr and Oct 2015 and Aug 2016) with no species restriction (65 519 838 to 93 482 448 sequences), trypsin as the cleavage enzyme, and carbamidomethyl as a fixed modification of cysteines and methionine oxidation as a variable modification.

    Liquid Chromatography with Mass Spectroscopy:

    Article Title: Porous polymer monolithic column with surface-bound gold nanoparticles for the capture and separation of cysteine-containing peptides
    Article Snippet: Water delivered by an EASYpure® II system (Barnstead, Boston, MA, USA) was used for micro-HPLC experiments, while commercial MS-grade water was used for LC-MS experiments. .. An Acclaim PepMap™ C18 Nanocolumn (3 μm particle size, 100 Å pore size, 15 cm × 75 μm I.D.), a product of Dionex Corp. (Sunnyvale, CA, USA) was used in this study.

    Article Title: ENHANCED SENSITIVITY OF LC-MS ANALYSIS OF PERMETHYLATED N-GLYCANS THROUGH ONLINE PURIFICATION
    Article Snippet: Paragraph title: On-line purification of permethylated N-glycans and LC-MS and LC-MSMS ... Next, samples were initially injected onto an Acclaim® PepMap100 C18 nano-trap column (Dionex, Sunnyvale, CA).

    Tandem Mass Spectroscopy:

    Article Title: Identification of the phosphorylation sites in the survival motor neuron protein by protein kinase A
    Article Snippet: Each sample was then separated on a Dionex 3000 nLC system (Sunnyvale, CA) equipped with an Acclaim PepMap 100 C18 trap column (300 μm × 5 mm, 5 μm, Dionex) and an Acclaim PepMap 100 C18 analytical column (75 μm × 15 cm, 3 μm, Dionex). .. Data acquisition consisted of a precursor scan targeting -79 m/z in negative ion mode and MS/MS of the corresponding five most intense ions from each survey scan in positive ion mode.

    Article Title: Not just fat: investigating the proteome of cetacean blubber tissue
    Article Snippet: These were analysed using nanoflow Liquid Chromatography Electrospray Ionization in tandem with Mass Spectrometry (nLC-ESI MS/MS) of in-gel trypsin digests. .. The peptides were then injected on an Acclaim PepMap 100 C18 trap and an Acclaim PepMap RSLC C18 column (ThermoFisher Scientific) using a nanoLC Ultra 2D plus loading pump and nanoLC as-2 autosampler (Eksigent).

    Modification:

    Article Title: Not just fat: investigating the proteome of cetacean blubber tissue
    Article Snippet: The peptides were then injected on an Acclaim PepMap 100 C18 trap and an Acclaim PepMap RSLC C18 column (ThermoFisher Scientific) using a nanoLC Ultra 2D plus loading pump and nanoLC as-2 autosampler (Eksigent). .. The eluate was sprayed into a TripleTOF 5600+ electrospray tandem mass spectrometer (Sciex, Foster City, CA) and analysed in Information Dependent Acquisition (IDA) mode, performing 250 ms of MS followed by 100 ms MSMS analyses on the 20 most intense peaks seen by MS The MS/MS data file generated via the ‘Create mgf file’ script in PeakView (Sciex) was analysed using the Mascot search algorithm (Matrix Science), against the NCBInr database (Apr and Oct 2015 and Aug 2016) with no species restriction (65 519 838 to 93 482 448 sequences), trypsin as the cleavage enzyme, and carbamidomethyl as a fixed modification of cysteines and methionine oxidation as a variable modification.

    Transformation Assay:

    Article Title: The Interplay of Light and Oxygen in the Reactive Oxygen Stress Response of Chlamydomonas reinhardtii Dissected by Quantitative Mass Spectrometry *
    Article Snippet: For peptide separation, the trap column was switched in-line with a C18 capillary column (Acclaim PepMap 100, 75 μm × 150 mm, 3 μm particle size, 100 Å pore size, Dionex) and the following gradient was applied: 0–35% B (85 min), 35–50% B (10 min), 50–100% B (5 min), 100% B (5 min). .. Finally, ions were generated by electrospray ionization (ESI) and full MS1 were measured in the Orbitrap, i.e. , a Fourier transformation mass spectrometer (mass range: 375–1800 m / z ; resolution: 60,000).

    Injection:

    Article Title: Homeolog expression analysis in an allotriploid non-model crop via integration of transcriptomics and proteomics
    Article Snippet: .. The digested samples (1 µg/5 µL) were injected and separated on an Ultimate 3000 UPLC system (Dionex, Thermo Scientific, USA) equipped with a Acclaim PepMap100 pre column (C18 3 µm-100 Å, Thermo Scientific, USA) and an C18 PepMap RSLC (2 µm, 50 × 50 cm, Thermo Scientific, USA) following a multiple step gradient with a linear gradient (flow rate: 0.300 µL/min): 4% buffer B (80% acetonitrile (CAN), in 0.08% formic acid (FA)) for 3 min, 4–12% in 17 min, 12–15% in 10 min, 15–18% in 45 min, 18–20% in 40 min, 20–30% in 65 min, 30–35% in 30 min, 35–65% in 10 min, 65–95% in 5 min, 95% for 40 min, 95–4% in 1 min, 4% for 39 min. .. The Q Exactive Orbitrap mass spectrometer (Thermo Scientific, USA) was operated in positive ion mode with a nano spray voltage of 1.5 kV and the source temperature set to 250 °C.

    Article Title: Potential role of new molecular plasma signatures on cardiovascular risk stratification in asymptomatic individuals
    Article Snippet: .. Digested samples (1 µg of protein and 25 fmol of each heavy-labeled peptide) were injected onto an Acclaim PepMap100 C18 trap column (100 um × 2 cm, Thermo scientific) and then separated by RP-HPLC on an Acclaim PepMap RSLC C18 column (75 um × 15 cm, Thermo scientific). .. Chromatography was performed with solvent A (0.1% FA) and solvent B (100% ACN, 0.1% FA) as a mobile phase, using at a 300 nl/min flow rate and a linear gradient: 60 minutes from 2% B to 40% B; 5 minutes to 50% B; and 5 minutes at 90% B before returning to 2% B).

    Article Title: Mass spectrometry based analysis of human plasma-derived factor X revealed novel post-translational modifications
    Article Snippet: .. Peptides obtained after digestion were injected on an Acclaim PepMap100 C18 column (0.075 × 150 mm, 3 µm) using a U3000 nano - LC system fitted with a preconcentration setup and interfaced with a high-resolution LTQ-Orbitrap Discovery mass spectrometer (Thermofischer Scientific, Bremen, Germany) operating in the positive ion mode. ..

    Article Title: ENHANCED SENSITIVITY OF LC-MS ANALYSIS OF PERMETHYLATED N-GLYCANS THROUGH ONLINE PURIFICATION
    Article Snippet: .. Next, samples were initially injected onto an Acclaim® PepMap100 C18 nano-trap column (Dionex, Sunnyvale, CA). .. The samples were then washed using mobile phase A, consisting of 98% HPLC grade water, 2% ACN, and 0.1% formic acid.

    Article Title: Discrimination of grade 2 and 3 cervical intraepithelial neoplasia by means of analysis of water soluble proteins recovered from cervical biopsies
    Article Snippet: × 15 cm Acclaim PepMap 100 C18 analytical column (Dionex) were used with a LTQ-Orbitrap hybrid mass spectrometer (Thermo Scientific). .. 5 μl of the tryptic digests were injected onto the trap column using 0.1% formic acid (FA, VWR) in MilliQ-water (Elga) at a flow of 2 μl/min.

    Article Title: Not just fat: investigating the proteome of cetacean blubber tissue
    Article Snippet: .. The peptides were then injected on an Acclaim PepMap 100 C18 trap and an Acclaim PepMap RSLC C18 column (ThermoFisher Scientific) using a nanoLC Ultra 2D plus loading pump and nanoLC as-2 autosampler (Eksigent). ..

    Derivative Assay:

    Article Title: ENHANCED SENSITIVITY OF LC-MS ANALYSIS OF PERMETHYLATED N-GLYCANS THROUGH ONLINE PURIFICATION
    Article Snippet: Next, samples were initially injected onto an Acclaim® PepMap100 C18 nano-trap column (Dionex, Sunnyvale, CA). .. The equivalence of N-glycans derived from 0.1 μg of the model glycoproteins was injected.

    Software:

    Article Title: Potential role of new molecular plasma signatures on cardiovascular risk stratification in asymptomatic individuals
    Article Snippet: Digested samples (1 µg of protein and 25 fmol of each heavy-labeled peptide) were injected onto an Acclaim PepMap100 C18 trap column (100 um × 2 cm, Thermo scientific) and then separated by RP-HPLC on an Acclaim PepMap RSLC C18 column (75 um × 15 cm, Thermo scientific). .. SRM data were acquired in positive mode with: a spray voltage of 2800 V; curtain gas 20 psi; ion source gas 20 psi; interface heater temperature (IHT) 150 °C; DP 80; entrance potential 10; exit potential (EXP) 15; and a pause time of 3 ms. Collision Energy (CE) was estimated using MRM Pilot software (ABsciex) and set according to Table .

    Article Title: The ovine hepatic mitochondrial proteome: Understanding seasonal weight loss tolerance in two distinct breeds
    Article Snippet: Samples were loaded onto a trapping cartridge (Acclaim PepMap C18 100A°, 5 mm x 300 μm i.d., 160454, Thermo Scientific) in a mobile phase of 2% ACN, 0.1% FA at 10 μL/min. .. Data acquisition was controlled by Xcalibur 4.0 and Tune 2.8 software (Thermo Scientific, Bremen, Germany).

    Article Title: The Interplay of Light and Oxygen in the Reactive Oxygen Stress Response of Chlamydomonas reinhardtii Dissected by Quantitative Mass Spectrometry *
    Article Snippet: Software versions LTQ (Tune) 2.0.7 and Xcalibur 2.0.7 were used. .. For peptide separation, the trap column was switched in-line with a C18 capillary column (Acclaim PepMap 100, 75 μm × 150 mm, 3 μm particle size, 100 Å pore size, Dionex) and the following gradient was applied: 0–35% B (85 min), 35–50% B (10 min), 50–100% B (5 min), 100% B (5 min).

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93
    Thermo Fisher c18 acclaim pepmap100
    C18 Acclaim Pepmap100, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 57 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/c18 acclaim pepmap100/product/Thermo Fisher
    Average 93 stars, based on 57 article reviews
    Price from $9.99 to $1999.99
    c18 acclaim pepmap100 - by Bioz Stars, 2020-04
    93/100 stars
      Buy from Supplier

    96
    Thermo Fisher reverse phase trap column
    Reverse Phase Trap Column, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/reverse phase trap column/product/Thermo Fisher
    Average 96 stars, based on 20 article reviews
    Price from $9.99 to $1999.99
    reverse phase trap column - by Bioz Stars, 2020-04
    96/100 stars
      Buy from Supplier

    98
    Thermo Fisher reversed phase trap column
    Reversed Phase Trap Column, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/reversed phase trap column/product/Thermo Fisher
    Average 98 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    reversed phase trap column - by Bioz Stars, 2020-04
    98/100 stars
      Buy from Supplier

    Image Search Results