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Agilent technologies c 18 reverse phase column
C 18 Reverse Phase Column, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 96/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Incubation:

Article Title: Proximal tubule apical endocytosis is modulated by fluid shear stress via an mTOR-dependent pathway
Article Snippet: After a 60-min incubation on ice, 15 μl of formic acid and 100 μl of ethyl acetate were added to the tubes. .. Aliquots of samples (2 μl) were injected into a C-18 reverse-phase column (Agilent Prep-C18 Scalar, 5 μm, 100 × 4.6 mm) protected by a guard cartridge.

Article Title: Structure-based Mechanistic Insights into Terminal Amide Synthase in Nosiheptide-Represented Thiopeptides Biosynthesis
Article Snippet: For simple comparison, 5 μL of diluted protein solution and 5 μL of substrate solution were added into 40 μL of buffer A, respectively, and then was incubated at 303 K for 60 min. To quench the reaction, 5 μL of methanol were added. .. To determine whether the product nosiheptide was generated or not, HPLC analysis was performed by running C-18 reverse phase column (Agilent 1100) with a MeCN/H2 O gradient mobile phase.

Article Title: A novel strain of Cellulosimicrobium funkei can biologically detoxify aflatoxin B1 in ducklings
Article Snippet: HPLC analysis was performed on a Shimadazu LC-20A binary gradient liquid chromatography equipped with a 5 μm × 4.6 mm × 250 mm C-18 reverse-phase column (ZORBAX Eclipse XDB-C18, Agilent). .. The sterilized LB medium alone substituted fermentation supernatant incubated with AFB1 solution was used as the negative control.

Synthesized:

Article Title: Engineering an antibody with picomolar affinity to DOTA chelates of multiple radionuclides for pretargeted radioimmunotherapy and imaging
Article Snippet: DOTA-Bn-biotin ( ) was synthesized by dissolving Amine-PEG3 -Biotin purchased from Thermo Scientific (Rockford, IL) and S-2-(4-Isothiocyanatobenzyl)-1,4,7,10-tetraazacyclododecane-tetraacetic acid (p-SCN-Bn-DOTA) purchased from Macrocyclics in dimethyl sulfoxide (DMSO) with a 10 fold molar excess of triethylamine. .. HPLC purification was performed on a C-18 reverse-phase column (Agilent Model 1100 HPLC, 1 × 25 cm, buffer A = 0.05% trifluoroacetic acid (TFA), buffer B = 0.0425% TFA in 80% acetonitrile, 2 – 100% B gradient for 98 min).

Article Title: A modular IgG-scFv bispecific antibody topology
Article Snippet: DOTA-biotin was synthesized by adding p-SCN-B n -DOTA ( S -2-(4-isothiocyanatobenzyl)-1,4,7,10-tetraazacyclododecane-tetraacetic acid) purchased from Macrocyclics (Dallas, TX) to Amine-PEG3 -Biotin (Thermo Fisher Scientific) in dimethyl sulfoxide (DMSO) purchased from Sigma Aldrich (St. Louis, MO) with a 10-fold molar excess of triethylamine (TEA) purchased from VWR (West Chester, PA). .. The reaction mixture was vortexed at room temperature for 3 h, and purified by high-performance liquid chromatography (HPLC) on a C-18 reverse-phase column (Agilent Model 1100 HPLC, 1 × 25 cm, buffer A = 0.05% trifluoroacetic acid (TFA), buffer B = 0.0425% TFA in 80% acetonitrile, 2–100% B gradient for 98 min).

Nuclear Magnetic Resonance:

Article Title: ?-Methylprednisolone conjugated cyclodextrin polymer-based nanoparticles for rheumatoid arthritis therapy
Article Snippet: NMR spectra were recorded on a Varian AMX 500 MHz or a Varian 300 MHz spectrometer. .. Release kinetics and polymer drug-loading of the MP derivative were analyzed with a C-18 reverse phase column on a Agilent 1100 HPLC system equipped with an UV detector at 250 nm using an isocratic mobile phase of potassium phosphate buffer (pH 4.1) and acetonitrile.

Article Title: Comprehensive Synthesis of Amino Acid-Derived Thiazole Peptidomimetic Analogues to Understand Enigmatic Drug/Substrate-Binding Site of P-glycoprotein
Article Snippet: The 13 C NMR (proton decoupled, fluorine coupled) data are presented as follows: chemical shift (multiplicity {d (doublet), t (triplet)}). .. Target compounds purity analysis was accomplished utilizing Agilent 1100 HPLC system with an autosampler (Agilent, Santa Clara, CA) eluting a C-18 reverse phase column (Agilent Eclipse plus C18, 3.5 μ m, 4.6 × 100 mm) with an isocratic mobile phase flow (1.0 mL/min), and samples monitored under UV light at 254 nm.

Article Title: Imaging Granzyme B Activity Assesses Immune-Mediated Myocarditis
Article Snippet: The loading of mPEG was quantified by NMR spectroscopy as 32 %. .. For analytical HPLC a C-18 reverse phase column (Agilent Pursuit XRS 10 μm) with dimensions of 250 mm × 4.6 mm was used at a flow rate of 1.0 mL/min.

Article Title: Imaging Granzyme B Activity Assesses Immune-Mediated Myocarditis
Article Snippet: For semi-preparative HPLC a C-18 reverse phase column (Agilent Pursuit XRS 10 μm) with dimensions of 250 mm × 21.2 mm was used with a flow rate of 21.0 mL/min. .. All 1 H NMR spectra (500 MHz, Varian) were collected in the solvents noted.

Activity Assay:

Article Title: A novel strain of Cellulosimicrobium funkei can biologically detoxify aflatoxin B1 in ducklings
Article Snippet: Single colonies that were able to grow on the coumarin (Sigma Chemical Co., Bellefonte, USA) plate were selected for AFB1 biodegradation activity analysis according to the protocol described by Guan and colleagues ( ) with minor modifications. .. HPLC analysis was performed on a Shimadazu LC-20A binary gradient liquid chromatography equipped with a 5 μm × 4.6 mm × 250 mm C-18 reverse-phase column (ZORBAX Eclipse XDB-C18, Agilent).

Mass Spectrometry:

Article Title: ?-Methylprednisolone conjugated cyclodextrin polymer-based nanoparticles for rheumatoid arthritis therapy
Article Snippet: Mass spectral (MS) analysis was performed using either an electrospray mass spectrometer equipped with LCQ ion trap (Thermo Finnigan, Waltham, MA) and fitted with an electrospray ionization source or a MALDI-TOF mass spectrometer (Voyager DE-PRO, Applied Biosystems, Foster City, CA). .. Release kinetics and polymer drug-loading of the MP derivative were analyzed with a C-18 reverse phase column on a Agilent 1100 HPLC system equipped with an UV detector at 250 nm using an isocratic mobile phase of potassium phosphate buffer (pH 4.1) and acetonitrile.

Article Title: Comprehensive Synthesis of Amino Acid-Derived Thiazole Peptidomimetic Analogues to Understand Enigmatic Drug/Substrate-Binding Site of P-glycoprotein
Article Snippet: Target compounds purity analysis was accomplished utilizing Agilent 1100 HPLC system with an autosampler (Agilent, Santa Clara, CA) eluting a C-18 reverse phase column (Agilent Eclipse plus C18, 3.5 μ m, 4.6 × 100 mm) with an isocratic mobile phase flow (1.0 mL/min), and samples monitored under UV light at 254 nm. .. Mass analyses were carried out on Agilent 1260 infinity series liquid chromatography (LC) system (C18 column, Agilent InfinityLab poroshell 120, EC-C18, 2.7 μ m, 4.6 × 50 mm) connected with Agilent 6120 quadrupole mass spectrometer (MS).

Article Title: Engineering an antibody with picomolar affinity to DOTA chelates of multiple radionuclides for pretargeted radioimmunotherapy and imaging
Article Snippet: HPLC purification was performed on a C-18 reverse-phase column (Agilent Model 1100 HPLC, 1 × 25 cm, buffer A = 0.05% trifluoroacetic acid (TFA), buffer B = 0.0425% TFA in 80% acetonitrile, 2 – 100% B gradient for 98 min). .. Fractions containing DOTA-Bn-biotin were confirmed using matrix assisted laser desorption instrument time of flight (MALDI-TOF) mass spectrometry (Applied Biosystems Model Voyager DE-STR).

Article Title: A modular IgG-scFv bispecific antibody topology
Article Snippet: The reaction mixture was vortexed at room temperature for 3 h, and purified by high-performance liquid chromatography (HPLC) on a C-18 reverse-phase column (Agilent Model 1100 HPLC, 1 × 25 cm, buffer A = 0.05% trifluoroacetic acid (TFA), buffer B = 0.0425% TFA in 80% acetonitrile, 2–100% B gradient for 98 min). .. Fractions containing DOTA-biotin were confirmed using matrix-assisted laser desorption/ionization–time of flight (MALDI-TOF) mass spectrometry (Applied Biosystems Model Voyager DE-STR).

Modification:

Article Title: A novel strain of Cellulosimicrobium funkei can biologically detoxify aflatoxin B1 in ducklings
Article Snippet: The AFB1 concentration was determined by HPLC (Teniola et al ., ) with a minor modification. .. HPLC analysis was performed on a Shimadazu LC-20A binary gradient liquid chromatography equipped with a 5 μm × 4.6 mm × 250 mm C-18 reverse-phase column (ZORBAX Eclipse XDB-C18, Agilent).

High Performance Liquid Chromatography:

Article Title: ?-Methylprednisolone conjugated cyclodextrin polymer-based nanoparticles for rheumatoid arthritis therapy
Article Snippet: .. Release kinetics and polymer drug-loading of the MP derivative were analyzed with a C-18 reverse phase column on a Agilent 1100 HPLC system equipped with an UV detector at 250 nm using an isocratic mobile phase of potassium phosphate buffer (pH 4.1) and acetonitrile. .. Particle size of CDP-MP was measured on Zeta PALS, Brookhaven Instrument Corporation (Holtsville, NY).

Article Title: Proximal tubule apical endocytosis is modulated by fluid shear stress via an mTOR-dependent pathway
Article Snippet: Reduced pyridine dinucleotides were also analyzed by HPLC. .. In this regard, aliquots of cell extracts (20 μl) were injected into a C-18 reverse-phase column (Agilent Prep-C18 Scalar, 5 μm, 100 × 4.6 mm) protected by a guard cartridge and connected with a fluorescence detector.

Article Title: Proximal tubule apical endocytosis is modulated by fluid shear stress via an mTOR-dependent pathway
Article Snippet: .. Analyses of adenine nucleotides were performed by HPLC with a C-18 reverse-phase column (Agilent Prep-C18 Scalar, 5 μm, 100 × 4.6 mm) protected by a guard cartridge in gradient mode (buffer A: 0.15 M KH2 PO4 [pH 6.2] in water; buffer B: 0.15 M KH2 PO4 [pH 6.2] in 15% acetonitrile; linear gradient [%B]): at 0 min 3.0%; from 0 to 9 min to 9.0%; from 9 to 25 min to 100.0%; from 25 to 30 min, 100.0%; from 30 to 31 min to 3.0%; from 31 to 35 min, 3.0%. ..

Article Title: Comprehensive Synthesis of Amino Acid-Derived Thiazole Peptidomimetic Analogues to Understand Enigmatic Drug/Substrate-Binding Site of P-glycoprotein
Article Snippet: .. Target compounds purity analysis was accomplished utilizing Agilent 1100 HPLC system with an autosampler (Agilent, Santa Clara, CA) eluting a C-18 reverse phase column (Agilent Eclipse plus C18, 3.5 μ m, 4.6 × 100 mm) with an isocratic mobile phase flow (1.0 mL/min), and samples monitored under UV light at 254 nm. ..

Article Title: Proximal tubule apical endocytosis is modulated by fluid shear stress via an mTOR-dependent pathway
Article Snippet: The bottom-phase aliquot (40 μl) was transferred into HPLC vials. .. Aliquots of samples (2 μl) were injected into a C-18 reverse-phase column (Agilent Prep-C18 Scalar, 5 μm, 100 × 4.6 mm) protected by a guard cartridge.

Article Title: Molecular Imaging of Mesothelioma with 99mTc-ECG and 68Ga-ECG
Article Snippet: .. High-performance liquid chromatography (HPLC), equipped with a NaI detector and UV detector (210 nm), was performed on a C-18 reverse phase column (C18-extend, Agilent, Santa Clara, CA) eluted with acetonitrile/water (1 : 9, V/V) at a flow rate of 0.5 mL/min. .. HPLC of cold 69 Ga-ECG was used to confirm the structure of 68 Ga-ECG.

Article Title: Structure-based Mechanistic Insights into Terminal Amide Synthase in Nosiheptide-Represented Thiopeptides Biosynthesis
Article Snippet: .. To determine whether the product nosiheptide was generated or not, HPLC analysis was performed by running C-18 reverse phase column (Agilent 1100) with a MeCN/H2 O gradient mobile phase. ..

Article Title: A novel strain of Cellulosimicrobium funkei can biologically detoxify aflatoxin B1 in ducklings
Article Snippet: .. HPLC analysis was performed on a Shimadazu LC-20A binary gradient liquid chromatography equipped with a 5 μm × 4.6 mm × 250 mm C-18 reverse-phase column (ZORBAX Eclipse XDB-C18, Agilent). .. The mobile phase was acetonitrile/methanol/water (1:1:2, v/v/v) at a flow rate of 1 ml/min, and the sample temperature was set at 30°C.

Article Title: Adenosine Production by Brain Cells
Article Snippet: .. Analyses of ATP, ADP and 5′-AMP were performed by HPLC with a C-18 reverse phase column (Agilent Prep-C18 Scalar, 5 μm, 100 × 4.6 mm) protected by a guard cartridge in gradient mode [buffer A: 0.15 M KH2 PO4 (pH 6.2) in water; buffer B: 0.15 M KH2 PO4 (pH 6.2) in 15% acetonitrile; linear gradient (%B): at 0 minutes 3.0%; from 0 to 9 minutes to 9.0%; from 9 to 25 minutes to 100.0%; from 25 to 30 minutes, 100.0%; from 30 to 31 minutes to 3.0%; from 31 to 35 minutes, 3.0%. ..

Article Title: Imaging Granzyme B Activity Assesses Immune-Mediated Myocarditis
Article Snippet: .. For analytical HPLC a C-18 reverse phase column (Agilent Pursuit XRS 10 μm) with dimensions of 250 mm × 4.6 mm was used at a flow rate of 1.0 mL/min. .. For semi-preparative HPLC a C-18 reverse phase column (Agilent Pursuit XRS 10 μm) with dimensions of 250 mm × 21.2 mm was used with a flow rate of 21.0 mL/min.

Article Title: Engineering an antibody with picomolar affinity to DOTA chelates of multiple radionuclides for pretargeted radioimmunotherapy and imaging
Article Snippet: .. HPLC purification was performed on a C-18 reverse-phase column (Agilent Model 1100 HPLC, 1 × 25 cm, buffer A = 0.05% trifluoroacetic acid (TFA), buffer B = 0.0425% TFA in 80% acetonitrile, 2 – 100% B gradient for 98 min). .. Flow through was monitored by absorbance detection at 280 nanometers.

Article Title: Imaging Granzyme B Activity Assesses Immune-Mediated Myocarditis
Article Snippet: .. For semi-preparative HPLC a C-18 reverse phase column (Agilent Pursuit XRS 10 μm) with dimensions of 250 mm × 21.2 mm was used with a flow rate of 21.0 mL/min. .. All 1 H NMR spectra (500 MHz, Varian) were collected in the solvents noted.

Article Title: A modular IgG-scFv bispecific antibody topology
Article Snippet: .. The reaction mixture was vortexed at room temperature for 3 h, and purified by high-performance liquid chromatography (HPLC) on a C-18 reverse-phase column (Agilent Model 1100 HPLC, 1 × 25 cm, buffer A = 0.05% trifluoroacetic acid (TFA), buffer B = 0.0425% TFA in 80% acetonitrile, 2–100% B gradient for 98 min). .. Flow through was monitored by absorbance detection at 280 nm.

Article Title: Sequence Diversity in Coding Regions of Candidate Genes in the Glycoalkaloid Biosynthetic Pathway of Wild Potato Species
Article Snippet: .. A high-performance liquid chromatography (Agilent HP 1200 Series, Santa Clara, CA) on a C-18 reverse-phase column (Agilent Eclipse XDB-C18, 5-µm pore size and 4.6 × 150 mm) procedure was used to separate the SGAs, and a photodiode array detector to quantified them. .. Elution of SGAs was attained by using a binary gradient system consisting of Solvent A (30% acetonitrile, 6 mM Tris-HCl, pH 8.0) and Solvent B (80% acetonitrile, 6 mM Tris-HCl, pH 7.6) at a flow rate of 0.3 mL/min at 25° column temperature.

Article Title: Design and Mechanism of (S)-3-Amino-4-(difluoromethylenyl)cyclopent-1-ene-1-carboxylic Acid, a Highly Potent GABA Aminotransferase Inactivator for the Treatment of Addiction
Article Snippet: .. An Agilent 1260 infinity HPLC system was used, which consists of a variable wavelength detector (G1314A), a thermostatted column compartment (G1316A), an autosampler (G1329B), an evaporative light scattering detector (ELSD, G4261A), a quaternary pump (G1311B), and a C-18 reverse phase column (Agilent Poroshell 120, 2.7 μm, 4.6 mm × 50 mm). ..

Flow Cytometry:

Article Title: ?-Methylprednisolone conjugated cyclodextrin polymer-based nanoparticles for rheumatoid arthritis therapy
Article Snippet: MWs of the polymer samples were analyzed on a GPC system equipped with Agilent 1100 Series HPLC system, a Wyatt DAWN Heleos, Wyatt Optical rex, and two gel permeation columns in series (PL-aquagel-OH-40 8 μm 300 × 7.5 mm and PL-aquagel-OH-50 8 μm 300 × 7.5 mm, Polymer Laboratories, Amherst, MA) calibrated using poly(ethyleneoxide) standard and eluted using PBS (1X) at a concentration of 6 mg/mL and at a 1.0 mL/min flow rate at 30 °C. .. Release kinetics and polymer drug-loading of the MP derivative were analyzed with a C-18 reverse phase column on a Agilent 1100 HPLC system equipped with an UV detector at 250 nm using an isocratic mobile phase of potassium phosphate buffer (pH 4.1) and acetonitrile.

Article Title: Comprehensive Synthesis of Amino Acid-Derived Thiazole Peptidomimetic Analogues to Understand Enigmatic Drug/Substrate-Binding Site of P-glycoprotein
Article Snippet: .. Target compounds purity analysis was accomplished utilizing Agilent 1100 HPLC system with an autosampler (Agilent, Santa Clara, CA) eluting a C-18 reverse phase column (Agilent Eclipse plus C18, 3.5 μ m, 4.6 × 100 mm) with an isocratic mobile phase flow (1.0 mL/min), and samples monitored under UV light at 254 nm. ..

Article Title: Molecular Imaging of Mesothelioma with 99mTc-ECG and 68Ga-ECG
Article Snippet: .. High-performance liquid chromatography (HPLC), equipped with a NaI detector and UV detector (210 nm), was performed on a C-18 reverse phase column (C18-extend, Agilent, Santa Clara, CA) eluted with acetonitrile/water (1 : 9, V/V) at a flow rate of 0.5 mL/min. .. HPLC of cold 69 Ga-ECG was used to confirm the structure of 68 Ga-ECG.

Article Title: A novel strain of Cellulosimicrobium funkei can biologically detoxify aflatoxin B1 in ducklings
Article Snippet: HPLC analysis was performed on a Shimadazu LC-20A binary gradient liquid chromatography equipped with a 5 μm × 4.6 mm × 250 mm C-18 reverse-phase column (ZORBAX Eclipse XDB-C18, Agilent). .. The mobile phase was acetonitrile/methanol/water (1:1:2, v/v/v) at a flow rate of 1 ml/min, and the sample temperature was set at 30°C.

Article Title: Imaging Granzyme B Activity Assesses Immune-Mediated Myocarditis
Article Snippet: .. For analytical HPLC a C-18 reverse phase column (Agilent Pursuit XRS 10 μm) with dimensions of 250 mm × 4.6 mm was used at a flow rate of 1.0 mL/min. .. For semi-preparative HPLC a C-18 reverse phase column (Agilent Pursuit XRS 10 μm) with dimensions of 250 mm × 21.2 mm was used with a flow rate of 21.0 mL/min.

Article Title: Engineering an antibody with picomolar affinity to DOTA chelates of multiple radionuclides for pretargeted radioimmunotherapy and imaging
Article Snippet: HPLC purification was performed on a C-18 reverse-phase column (Agilent Model 1100 HPLC, 1 × 25 cm, buffer A = 0.05% trifluoroacetic acid (TFA), buffer B = 0.0425% TFA in 80% acetonitrile, 2 – 100% B gradient for 98 min). .. Flow through was monitored by absorbance detection at 280 nanometers.

Article Title: Imaging Granzyme B Activity Assesses Immune-Mediated Myocarditis
Article Snippet: .. For semi-preparative HPLC a C-18 reverse phase column (Agilent Pursuit XRS 10 μm) with dimensions of 250 mm × 21.2 mm was used with a flow rate of 21.0 mL/min. .. All 1 H NMR spectra (500 MHz, Varian) were collected in the solvents noted.

Article Title: A modular IgG-scFv bispecific antibody topology
Article Snippet: The reaction mixture was vortexed at room temperature for 3 h, and purified by high-performance liquid chromatography (HPLC) on a C-18 reverse-phase column (Agilent Model 1100 HPLC, 1 × 25 cm, buffer A = 0.05% trifluoroacetic acid (TFA), buffer B = 0.0425% TFA in 80% acetonitrile, 2–100% B gradient for 98 min). .. Flow through was monitored by absorbance detection at 280 nm.

Article Title: Sequence Diversity in Coding Regions of Candidate Genes in the Glycoalkaloid Biosynthetic Pathway of Wild Potato Species
Article Snippet: A high-performance liquid chromatography (Agilent HP 1200 Series, Santa Clara, CA) on a C-18 reverse-phase column (Agilent Eclipse XDB-C18, 5-µm pore size and 4.6 × 150 mm) procedure was used to separate the SGAs, and a photodiode array detector to quantified them. .. Elution of SGAs was attained by using a binary gradient system consisting of Solvent A (30% acetonitrile, 6 mM Tris-HCl, pH 8.0) and Solvent B (80% acetonitrile, 6 mM Tris-HCl, pH 7.6) at a flow rate of 0.3 mL/min at 25° column temperature.

Chromatography:

Article Title: Comprehensive Synthesis of Amino Acid-Derived Thiazole Peptidomimetic Analogues to Understand Enigmatic Drug/Substrate-Binding Site of P-glycoprotein
Article Snippet: Column chromatography purifications were performed with silica gel (40–63 μ m) obtained from Silicycle Inc. (Quebec City, Canada) and flash chromatography was performed using Reveleris® X2 flash chromatography system (BÜCHI Corporation, New Castle, DE). .. Target compounds purity analysis was accomplished utilizing Agilent 1100 HPLC system with an autosampler (Agilent, Santa Clara, CA) eluting a C-18 reverse phase column (Agilent Eclipse plus C18, 3.5 μ m, 4.6 × 100 mm) with an isocratic mobile phase flow (1.0 mL/min), and samples monitored under UV light at 254 nm.

Article Title: Adenosine Production by Brain Cells
Article Snippet: Therefore, to address the basal cellular energy charges [(ATP+ 1/2ADP)/(ATP+ADP+5′-AMP)] for neurons, astrocytes, and microglia, we developed a high pressure liquid chromatography-ultraviolet absorbance (HPLC-UV) assay. .. Analyses of ATP, ADP and 5′-AMP were performed by HPLC with a C-18 reverse phase column (Agilent Prep-C18 Scalar, 5 μm, 100 × 4.6 mm) protected by a guard cartridge in gradient mode [buffer A: 0.15 M KH2 PO4 (pH 6.2) in water; buffer B: 0.15 M KH2 PO4 (pH 6.2) in 15% acetonitrile; linear gradient (%B): at 0 minutes 3.0%; from 0 to 9 minutes to 9.0%; from 9 to 25 minutes to 100.0%; from 25 to 30 minutes, 100.0%; from 30 to 31 minutes to 3.0%; from 31 to 35 minutes, 3.0%.

Article Title: Imaging Granzyme B Activity Assesses Immune-Mediated Myocarditis
Article Snippet: Liquid chromatography-mass spectrometry (LCMS) data were collected with a Waters 2695 high-performance liquid chromatography (HPLC) system (Milford, MA) equipped with a 2996 diode array detector, a Micromass ZQ4000 ESI-MS module, and an Agilent Pursuit XRS5 100 × 2.0 mm column at a flow rate of 0.3 mL/min. .. For analytical HPLC a C-18 reverse phase column (Agilent Pursuit XRS 10 μm) with dimensions of 250 mm × 4.6 mm was used at a flow rate of 1.0 mL/min.

Cell Culture:

Article Title: A novel strain of Cellulosimicrobium funkei can biologically detoxify aflatoxin B1 in ducklings
Article Snippet: Initially, candidate isolates were cultured at 37°C in LB medium for 72 h, and then 950 μl fermented supernatant was taken and mixed with 50 μl 10 μg/ml AFB1 solution (Sigma Chemical Co., Bellefonte, USA) in a sterilized centrifuge tube, and then biodegradation tests were conducted at 37°C for 72 h. Finally, the reaction solution was centrifuged at 10 000 g at 4°C for 10 min to remove cells and the supernatant, and then it was collected for AFB1 quantification (Guan et al ., ). .. HPLC analysis was performed on a Shimadazu LC-20A binary gradient liquid chromatography equipped with a 5 μm × 4.6 mm × 250 mm C-18 reverse-phase column (ZORBAX Eclipse XDB-C18, Agilent).

Generated:

Article Title: Structure-based Mechanistic Insights into Terminal Amide Synthase in Nosiheptide-Represented Thiopeptides Biosynthesis
Article Snippet: .. To determine whether the product nosiheptide was generated or not, HPLC analysis was performed by running C-18 reverse phase column (Agilent 1100) with a MeCN/H2 O gradient mobile phase. ..

Sequencing:

Article Title: Structure-based Mechanistic Insights into Terminal Amide Synthase in Nosiheptide-Represented Thiopeptides Biosynthesis
Article Snippet: To determine whether the product nosiheptide was generated or not, HPLC analysis was performed by running C-18 reverse phase column (Agilent 1100) with a MeCN/H2 O gradient mobile phase. .. To investigate the importance of the sequence and the coiled-coil conformation of the C-terminal NosA112-151 in the catalytic reaction, three randomly selected peptides (Did2, Vps60 and JmjN from KDM5B) available in the lab were used to replace NosA112-151 , and to mix with the N-terminal NosA1-111 variant.

Article Title: Sequence Diversity in Coding Regions of Candidate Genes in the Glycoalkaloid Biosynthetic Pathway of Wild Potato Species
Article Snippet: Then, the leaf extract was applied followed by a sequence of washes: 7.5 mL of water, 5 mL of 50 mM ammonium bicarbonate (NH4 HCO3 ), 5 mL of 50 mM NH4 HCO3 :MeOH (1:1 v/v), and 7.5 mL of water. .. A high-performance liquid chromatography (Agilent HP 1200 Series, Santa Clara, CA) on a C-18 reverse-phase column (Agilent Eclipse XDB-C18, 5-µm pore size and 4.6 × 150 mm) procedure was used to separate the SGAs, and a photodiode array detector to quantified them.

Injection:

Article Title: Proximal tubule apical endocytosis is modulated by fluid shear stress via an mTOR-dependent pathway
Article Snippet: .. In this regard, aliquots of cell extracts (20 μl) were injected into a C-18 reverse-phase column (Agilent Prep-C18 Scalar, 5 μm, 100 × 4.6 mm) protected by a guard cartridge and connected with a fluorescence detector. ..

Article Title: Proximal tubule apical endocytosis is modulated by fluid shear stress via an mTOR-dependent pathway
Article Snippet: .. Aliquots of samples (2 μl) were injected into a C-18 reverse-phase column (Agilent Prep-C18 Scalar, 5 μm, 100 × 4.6 mm) protected by a guard cartridge. ..

Enzymatic Assay:

Article Title: Structure-based Mechanistic Insights into Terminal Amide Synthase in Nosiheptide-Represented Thiopeptides Biosynthesis
Article Snippet: Paragraph title: The enzymatic assay on HPLC system ... To determine whether the product nosiheptide was generated or not, HPLC analysis was performed by running C-18 reverse phase column (Agilent 1100) with a MeCN/H2 O gradient mobile phase.

Fluorescence:

Article Title: Proximal tubule apical endocytosis is modulated by fluid shear stress via an mTOR-dependent pathway
Article Snippet: .. In this regard, aliquots of cell extracts (20 μl) were injected into a C-18 reverse-phase column (Agilent Prep-C18 Scalar, 5 μm, 100 × 4.6 mm) protected by a guard cartridge and connected with a fluorescence detector. ..

Article Title: Proximal tubule apical endocytosis is modulated by fluid shear stress via an mTOR-dependent pathway
Article Snippet: Shimadzu LC-100AT vp HPLC chromatograph equipped with fluorescence detector (model RF-10Axl) and autosampler (model SIL-10AD vp) was used. .. Analyses of adenine nucleotides were performed by HPLC with a C-18 reverse-phase column (Agilent Prep-C18 Scalar, 5 μm, 100 × 4.6 mm) protected by a guard cartridge in gradient mode (buffer A: 0.15 M KH2 PO4 [pH 6.2] in water; buffer B: 0.15 M KH2 PO4 [pH 6.2] in 15% acetonitrile; linear gradient [%B]): at 0 min 3.0%; from 0 to 9 min to 9.0%; from 9 to 25 min to 100.0%; from 25 to 30 min, 100.0%; from 30 to 31 min to 3.0%; from 31 to 35 min, 3.0%.

Article Title: Proximal tubule apical endocytosis is modulated by fluid shear stress via an mTOR-dependent pathway
Article Snippet: Oxidized pyridine dinucleotides were converted into fluorescent derivatives with acetophenone and measured by HPLC with fluorescence detection ( ). .. Aliquots of samples (2 μl) were injected into a C-18 reverse-phase column (Agilent Prep-C18 Scalar, 5 μm, 100 × 4.6 mm) protected by a guard cartridge.

Article Title: Imaging Granzyme B Activity Assesses Immune-Mediated Myocarditis
Article Snippet: Fluorescence data were collected with a Varian Cary Eclipse fluorescence spectrophotometer (Palo Alto, CA). .. For analytical HPLC a C-18 reverse phase column (Agilent Pursuit XRS 10 μm) with dimensions of 250 mm × 4.6 mm was used at a flow rate of 1.0 mL/min.

Isolation:

Article Title: A novel strain of Cellulosimicrobium funkei can biologically detoxify aflatoxin B1 in ducklings
Article Snippet: Paragraph title: Soil samples and AFB1 biodegradation microorganism isolation ... HPLC analysis was performed on a Shimadazu LC-20A binary gradient liquid chromatography equipped with a 5 μm × 4.6 mm × 250 mm C-18 reverse-phase column (ZORBAX Eclipse XDB-C18, Agilent).

Purification:

Article Title: Engineering an antibody with picomolar affinity to DOTA chelates of multiple radionuclides for pretargeted radioimmunotherapy and imaging
Article Snippet: .. HPLC purification was performed on a C-18 reverse-phase column (Agilent Model 1100 HPLC, 1 × 25 cm, buffer A = 0.05% trifluoroacetic acid (TFA), buffer B = 0.0425% TFA in 80% acetonitrile, 2 – 100% B gradient for 98 min). .. Flow through was monitored by absorbance detection at 280 nanometers.

Article Title: A modular IgG-scFv bispecific antibody topology
Article Snippet: .. The reaction mixture was vortexed at room temperature for 3 h, and purified by high-performance liquid chromatography (HPLC) on a C-18 reverse-phase column (Agilent Model 1100 HPLC, 1 × 25 cm, buffer A = 0.05% trifluoroacetic acid (TFA), buffer B = 0.0425% TFA in 80% acetonitrile, 2–100% B gradient for 98 min). .. Flow through was monitored by absorbance detection at 280 nm.

Article Title: Sequence Diversity in Coding Regions of Candidate Genes in the Glycoalkaloid Biosynthetic Pathway of Wild Potato Species
Article Snippet: SGAs were concentrated and purified by solid-phase extraction using Sep-Pak Classic C18 cartridge columns (Waters, Milford, MA). .. A high-performance liquid chromatography (Agilent HP 1200 Series, Santa Clara, CA) on a C-18 reverse-phase column (Agilent Eclipse XDB-C18, 5-µm pore size and 4.6 × 150 mm) procedure was used to separate the SGAs, and a photodiode array detector to quantified them.

Spectroscopy:

Article Title: Imaging Granzyme B Activity Assesses Immune-Mediated Myocarditis
Article Snippet: The loading of mPEG was quantified by NMR spectroscopy as 32 %. .. For analytical HPLC a C-18 reverse phase column (Agilent Pursuit XRS 10 μm) with dimensions of 250 mm × 4.6 mm was used at a flow rate of 1.0 mL/min.

Liquid Chromatography:

Article Title: Comprehensive Synthesis of Amino Acid-Derived Thiazole Peptidomimetic Analogues to Understand Enigmatic Drug/Substrate-Binding Site of P-glycoprotein
Article Snippet: Target compounds purity analysis was accomplished utilizing Agilent 1100 HPLC system with an autosampler (Agilent, Santa Clara, CA) eluting a C-18 reverse phase column (Agilent Eclipse plus C18, 3.5 μ m, 4.6 × 100 mm) with an isocratic mobile phase flow (1.0 mL/min), and samples monitored under UV light at 254 nm. .. Mass analyses were carried out on Agilent 1260 infinity series liquid chromatography (LC) system (C18 column, Agilent InfinityLab poroshell 120, EC-C18, 2.7 μ m, 4.6 × 50 mm) connected with Agilent 6120 quadrupole mass spectrometer (MS).

Article Title: A novel strain of Cellulosimicrobium funkei can biologically detoxify aflatoxin B1 in ducklings
Article Snippet: .. HPLC analysis was performed on a Shimadazu LC-20A binary gradient liquid chromatography equipped with a 5 μm × 4.6 mm × 250 mm C-18 reverse-phase column (ZORBAX Eclipse XDB-C18, Agilent). .. The mobile phase was acetonitrile/methanol/water (1:1:2, v/v/v) at a flow rate of 1 ml/min, and the sample temperature was set at 30°C.

Software:

Article Title: Proximal tubule apical endocytosis is modulated by fluid shear stress via an mTOR-dependent pathway
Article Snippet: Chromatograms were processed and stored in digital form with Class-VP software. .. In this regard, aliquots of cell extracts (20 μl) were injected into a C-18 reverse-phase column (Agilent Prep-C18 Scalar, 5 μm, 100 × 4.6 mm) protected by a guard cartridge and connected with a fluorescence detector.

Article Title: Proximal tubule apical endocytosis is modulated by fluid shear stress via an mTOR-dependent pathway
Article Snippet: Chromatograms were processed and stored in digital form with Class-VP software. .. Analyses of adenine nucleotides were performed by HPLC with a C-18 reverse-phase column (Agilent Prep-C18 Scalar, 5 μm, 100 × 4.6 mm) protected by a guard cartridge in gradient mode (buffer A: 0.15 M KH2 PO4 [pH 6.2] in water; buffer B: 0.15 M KH2 PO4 [pH 6.2] in 15% acetonitrile; linear gradient [%B]): at 0 min 3.0%; from 0 to 9 min to 9.0%; from 9 to 25 min to 100.0%; from 25 to 30 min, 100.0%; from 30 to 31 min to 3.0%; from 31 to 35 min, 3.0%.

Negative Control:

Article Title: A novel strain of Cellulosimicrobium funkei can biologically detoxify aflatoxin B1 in ducklings
Article Snippet: HPLC analysis was performed on a Shimadazu LC-20A binary gradient liquid chromatography equipped with a 5 μm × 4.6 mm × 250 mm C-18 reverse-phase column (ZORBAX Eclipse XDB-C18, Agilent). .. The sterilized LB medium alone substituted fermentation supernatant incubated with AFB1 solution was used as the negative control.

Column Chromatography:

Article Title: Comprehensive Synthesis of Amino Acid-Derived Thiazole Peptidomimetic Analogues to Understand Enigmatic Drug/Substrate-Binding Site of P-glycoprotein
Article Snippet: Column chromatography purifications were performed with silica gel (40–63 μ m) obtained from Silicycle Inc. (Quebec City, Canada) and flash chromatography was performed using Reveleris® X2 flash chromatography system (BÜCHI Corporation, New Castle, DE). .. Target compounds purity analysis was accomplished utilizing Agilent 1100 HPLC system with an autosampler (Agilent, Santa Clara, CA) eluting a C-18 reverse phase column (Agilent Eclipse plus C18, 3.5 μ m, 4.6 × 100 mm) with an isocratic mobile phase flow (1.0 mL/min), and samples monitored under UV light at 254 nm.

Gel Permeation Chromatography:

Article Title: ?-Methylprednisolone conjugated cyclodextrin polymer-based nanoparticles for rheumatoid arthritis therapy
Article Snippet: MWs of the polymer samples were analyzed on a GPC system equipped with Agilent 1100 Series HPLC system, a Wyatt DAWN Heleos, Wyatt Optical rex, and two gel permeation columns in series (PL-aquagel-OH-40 8 μm 300 × 7.5 mm and PL-aquagel-OH-50 8 μm 300 × 7.5 mm, Polymer Laboratories, Amherst, MA) calibrated using poly(ethyleneoxide) standard and eluted using PBS (1X) at a concentration of 6 mg/mL and at a 1.0 mL/min flow rate at 30 °C. .. Release kinetics and polymer drug-loading of the MP derivative were analyzed with a C-18 reverse phase column on a Agilent 1100 HPLC system equipped with an UV detector at 250 nm using an isocratic mobile phase of potassium phosphate buffer (pH 4.1) and acetonitrile.

Quantitation Assay:

Article Title: Proximal tubule apical endocytosis is modulated by fluid shear stress via an mTOR-dependent pathway
Article Snippet: Paragraph title: Extraction and quantitation of NADH, NAD+ , ATP, ADP, and 5′-AMP ... In this regard, aliquots of cell extracts (20 μl) were injected into a C-18 reverse-phase column (Agilent Prep-C18 Scalar, 5 μm, 100 × 4.6 mm) protected by a guard cartridge and connected with a fluorescence detector.

Spectrophotometry:

Article Title: Imaging Granzyme B Activity Assesses Immune-Mediated Myocarditis
Article Snippet: Fluorescence data were collected with a Varian Cary Eclipse fluorescence spectrophotometer (Palo Alto, CA). .. For analytical HPLC a C-18 reverse phase column (Agilent Pursuit XRS 10 μm) with dimensions of 250 mm × 4.6 mm was used at a flow rate of 1.0 mL/min.

Concentration Assay:

Article Title: ?-Methylprednisolone conjugated cyclodextrin polymer-based nanoparticles for rheumatoid arthritis therapy
Article Snippet: MWs of the polymer samples were analyzed on a GPC system equipped with Agilent 1100 Series HPLC system, a Wyatt DAWN Heleos, Wyatt Optical rex, and two gel permeation columns in series (PL-aquagel-OH-40 8 μm 300 × 7.5 mm and PL-aquagel-OH-50 8 μm 300 × 7.5 mm, Polymer Laboratories, Amherst, MA) calibrated using poly(ethyleneoxide) standard and eluted using PBS (1X) at a concentration of 6 mg/mL and at a 1.0 mL/min flow rate at 30 °C. .. Release kinetics and polymer drug-loading of the MP derivative were analyzed with a C-18 reverse phase column on a Agilent 1100 HPLC system equipped with an UV detector at 250 nm using an isocratic mobile phase of potassium phosphate buffer (pH 4.1) and acetonitrile.

Article Title: A novel strain of Cellulosimicrobium funkei can biologically detoxify aflatoxin B1 in ducklings
Article Snippet: The AFB1 concentration was determined by HPLC (Teniola et al ., ) with a minor modification. .. HPLC analysis was performed on a Shimadazu LC-20A binary gradient liquid chromatography equipped with a 5 μm × 4.6 mm × 250 mm C-18 reverse-phase column (ZORBAX Eclipse XDB-C18, Agilent).

Thin Layer Chromatography:

Article Title: Comprehensive Synthesis of Amino Acid-Derived Thiazole Peptidomimetic Analogues to Understand Enigmatic Drug/Substrate-Binding Site of P-glycoprotein
Article Snippet: Preparative TLC was performed using Silica Gel GF 1000 μ m 20×20cm glass backed plates from Analtech (Miles Scientific, Newark, DE). .. Target compounds purity analysis was accomplished utilizing Agilent 1100 HPLC system with an autosampler (Agilent, Santa Clara, CA) eluting a C-18 reverse phase column (Agilent Eclipse plus C18, 3.5 μ m, 4.6 × 100 mm) with an isocratic mobile phase flow (1.0 mL/min), and samples monitored under UV light at 254 nm.

Article Title: Molecular Imaging of Mesothelioma with 99mTc-ECG and 68Ga-ECG
Article Snippet: Radiochemical purity was determined by TLC (Waterman No. 1, Aldrich-Sigma, St. Louis, MO) eluted with saline. .. High-performance liquid chromatography (HPLC), equipped with a NaI detector and UV detector (210 nm), was performed on a C-18 reverse phase column (C18-extend, Agilent, Santa Clara, CA) eluted with acetonitrile/water (1 : 9, V/V) at a flow rate of 0.5 mL/min.

Liquid Chromatography with Mass Spectroscopy:

Article Title: Imaging Granzyme B Activity Assesses Immune-Mediated Myocarditis
Article Snippet: Liquid chromatography-mass spectrometry (LCMS) data were collected with a Waters 2695 high-performance liquid chromatography (HPLC) system (Milford, MA) equipped with a 2996 diode array detector, a Micromass ZQ4000 ESI-MS module, and an Agilent Pursuit XRS5 100 × 2.0 mm column at a flow rate of 0.3 mL/min. .. For analytical HPLC a C-18 reverse phase column (Agilent Pursuit XRS 10 μm) with dimensions of 250 mm × 4.6 mm was used at a flow rate of 1.0 mL/min.

Variant Assay:

Article Title: Structure-based Mechanistic Insights into Terminal Amide Synthase in Nosiheptide-Represented Thiopeptides Biosynthesis
Article Snippet: The enzymatic assay on HPLC system To assay the activities of the full-length NosA, NocA and their variants, the truncated N-terminal NosA1-111 variant, the mixture of NosA1-111 with the C-terminal NosA112-151 peptide, each protein solution (including the full-length NosA, NocA or their mutants, or the mixture of NosA1-111 with NosA112-151 at mole ratio of 1:1.) was diluted to 1 mg/ml in buffer A (50 mM Glycine buffer, pH 9.0). .. To determine whether the product nosiheptide was generated or not, HPLC analysis was performed by running C-18 reverse phase column (Agilent 1100) with a MeCN/H2 O gradient mobile phase.

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  • 96
    Agilent technologies reverse phase c18 column
    Orm1/2 are regulated in response to disruption of sphingolipid synthesis a , Serial dilutions of the indicated strains were spotted on plates with 0, 200, or 400 ng/ml myriocin and imaged after growth for 24–48 hr. b , LCBs were extracted and analyzed from wild-type (WT) and ORM1/2 deletion strains grown in media supplemented with the indicated concentrations of myriocin. The sums of <t>C18</t> dihydrosphingosine and phytosphingosine peak intensities from n ≥ 2 experiments are shown (relative to wild-type LCB levels in the absence of myriocin). c , Native immunoprecipitations of 3×Flag-tagged Orm1 and Orm2 were performed from strains grown in standard media or media supplemented with 150 ng/ml myriocin and analyzed by Western blot. The indicated strains also expressed 3×HA-Orm1/2 from their endogenous loci (diploid strains were used to examine self-association of Orm1 and Orm2). d , Lysates were prepared from yeast expressing 3×Flag-Orm1/2 after growth in media containing the indicated concentrations of myriocin. Western blots show phosphorylated forms of 3×Flag-Orm1 (P-Orm1) and 3×Flag-Orm2 (P-Orm2) after separation on phosphate-affinity gels 35 . An immunoprecipitated (IP) sample treated with calf intestine phosphatase (CIP) shows the position of un-phosphorylated Orm1/2.
    Reverse Phase C18 Column, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 96/100, based on 135 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    83
    Agilent technologies reversed phase xdb c18
    Glutamine is the death-inducing small molecule component in serum ( A ) Purification scheme for the death-inducing small molecule component in FBS. S: supernatant; P: pellet. See Methods for detailed description. ( B ) LC/MS spectra of the active fraction from the <t>XDB-C18</t> column. ( C ) L-Gln (left panel) but not D-Gln (right panel) induces cell death in a diFBS-dependent manner under AA starvation. MEFs were treated as indicated for 12 hrs and cell viability was subsequently measured. ( D ) L-Gln in combination with transferrin recapitulated the cell death-inducing activity of serum. bax/bak -DKO MEFs were treated as indicated for 12 hrs and cell viability was subsequently measured. L-Gln concentration, 0.1 mM.
    Reversed Phase Xdb C18, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 83/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Agilent technologies reversed phase c18 column
    Glutamine is the death-inducing small molecule component in serum ( A ) Purification scheme for the death-inducing small molecule component in FBS. S: supernatant; P: pellet. See Methods for detailed description. ( B ) LC/MS spectra of the active fraction from the <t>XDB-C18</t> column. ( C ) L-Gln (left panel) but not D-Gln (right panel) induces cell death in a diFBS-dependent manner under AA starvation. MEFs were treated as indicated for 12 hrs and cell viability was subsequently measured. ( D ) L-Gln in combination with transferrin recapitulated the cell death-inducing activity of serum. bax/bak -DKO MEFs were treated as indicated for 12 hrs and cell viability was subsequently measured. L-Gln concentration, 0.1 mM.
    Reversed Phase C18 Column, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
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    Orm1/2 are regulated in response to disruption of sphingolipid synthesis a , Serial dilutions of the indicated strains were spotted on plates with 0, 200, or 400 ng/ml myriocin and imaged after growth for 24–48 hr. b , LCBs were extracted and analyzed from wild-type (WT) and ORM1/2 deletion strains grown in media supplemented with the indicated concentrations of myriocin. The sums of C18 dihydrosphingosine and phytosphingosine peak intensities from n ≥ 2 experiments are shown (relative to wild-type LCB levels in the absence of myriocin). c , Native immunoprecipitations of 3×Flag-tagged Orm1 and Orm2 were performed from strains grown in standard media or media supplemented with 150 ng/ml myriocin and analyzed by Western blot. The indicated strains also expressed 3×HA-Orm1/2 from their endogenous loci (diploid strains were used to examine self-association of Orm1 and Orm2). d , Lysates were prepared from yeast expressing 3×Flag-Orm1/2 after growth in media containing the indicated concentrations of myriocin. Western blots show phosphorylated forms of 3×Flag-Orm1 (P-Orm1) and 3×Flag-Orm2 (P-Orm2) after separation on phosphate-affinity gels 35 . An immunoprecipitated (IP) sample treated with calf intestine phosphatase (CIP) shows the position of un-phosphorylated Orm1/2.

    Journal: Nature

    Article Title: Orm family proteins mediate sphingolipid homeostasis

    doi: 10.1038/nature08787

    Figure Lengend Snippet: Orm1/2 are regulated in response to disruption of sphingolipid synthesis a , Serial dilutions of the indicated strains were spotted on plates with 0, 200, or 400 ng/ml myriocin and imaged after growth for 24–48 hr. b , LCBs were extracted and analyzed from wild-type (WT) and ORM1/2 deletion strains grown in media supplemented with the indicated concentrations of myriocin. The sums of C18 dihydrosphingosine and phytosphingosine peak intensities from n ≥ 2 experiments are shown (relative to wild-type LCB levels in the absence of myriocin). c , Native immunoprecipitations of 3×Flag-tagged Orm1 and Orm2 were performed from strains grown in standard media or media supplemented with 150 ng/ml myriocin and analyzed by Western blot. The indicated strains also expressed 3×HA-Orm1/2 from their endogenous loci (diploid strains were used to examine self-association of Orm1 and Orm2). d , Lysates were prepared from yeast expressing 3×Flag-Orm1/2 after growth in media containing the indicated concentrations of myriocin. Western blots show phosphorylated forms of 3×Flag-Orm1 (P-Orm1) and 3×Flag-Orm2 (P-Orm2) after separation on phosphate-affinity gels 35 . An immunoprecipitated (IP) sample treated with calf intestine phosphatase (CIP) shows the position of un-phosphorylated Orm1/2.

    Article Snippet: Samples were analyzed on an Agilent Chemstation HPLC using a reverse-phase C18 column (ZORBAX Eclipse XDB-C18; Agilent).

    Techniques: Western Blot, Expressing, Immunoprecipitation

    Representative HPLC analyses of mouse plasma HPLC to detect S1P and sphinganine 1-phosphate after liver IR. Mice were subjected to either sham-operation or to liver ischemia and reperfusion. By introducing a known quantity of C17-S1P as an internal standard (Int Std), the amount of endogenous C18 S1P as well as C18 sphinganine 1-phosphate present in the original sample can be quantified.

    Journal: Shock (Augusta, Ga.)

    Article Title: Sphinganine-1-phosphate attenuates both hepatic and renal injury induced by hepatic ischemia and reperfusion in mice

    doi: 10.1097/SHK.0b013e3181c02c1f

    Figure Lengend Snippet: Representative HPLC analyses of mouse plasma HPLC to detect S1P and sphinganine 1-phosphate after liver IR. Mice were subjected to either sham-operation or to liver ischemia and reperfusion. By introducing a known quantity of C17-S1P as an internal standard (Int Std), the amount of endogenous C18 S1P as well as C18 sphinganine 1-phosphate present in the original sample can be quantified.

    Article Snippet: Chromatographic separation was achieved on a C18 reversed-phase column (Zorbax SB-C18, 3.5 μm; 4.6 × 150 mm, Agilent Technologies, Santa Clara, CA) and a mobile phase consisting of acetonitrile: deionized distilled water (89:11, v/v) at a flow rate of 0.8 ml/min.

    Techniques: High Performance Liquid Chromatography, Mouse Assay

    Glutamine is the death-inducing small molecule component in serum ( A ) Purification scheme for the death-inducing small molecule component in FBS. S: supernatant; P: pellet. See Methods for detailed description. ( B ) LC/MS spectra of the active fraction from the XDB-C18 column. ( C ) L-Gln (left panel) but not D-Gln (right panel) induces cell death in a diFBS-dependent manner under AA starvation. MEFs were treated as indicated for 12 hrs and cell viability was subsequently measured. ( D ) L-Gln in combination with transferrin recapitulated the cell death-inducing activity of serum. bax/bak -DKO MEFs were treated as indicated for 12 hrs and cell viability was subsequently measured. L-Gln concentration, 0.1 mM.

    Journal: Molecular cell

    Article Title: Glutaminolysis and Transferrin Regulate Ferroptosis

    doi: 10.1016/j.molcel.2015.06.011

    Figure Lengend Snippet: Glutamine is the death-inducing small molecule component in serum ( A ) Purification scheme for the death-inducing small molecule component in FBS. S: supernatant; P: pellet. See Methods for detailed description. ( B ) LC/MS spectra of the active fraction from the XDB-C18 column. ( C ) L-Gln (left panel) but not D-Gln (right panel) induces cell death in a diFBS-dependent manner under AA starvation. MEFs were treated as indicated for 12 hrs and cell viability was subsequently measured. ( D ) L-Gln in combination with transferrin recapitulated the cell death-inducing activity of serum. bax/bak -DKO MEFs were treated as indicated for 12 hrs and cell viability was subsequently measured. L-Gln concentration, 0.1 mM.

    Article Snippet: An aliquot of 50-μl as input was applied to a reversed phase XDB-C18 (4.6 × 250 mm) HPLC analytical column (Agilent).

    Techniques: Purification, Liquid Chromatography with Mass Spectroscopy, Activity Assay, Concentration Assay