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Agilent technologies c 18 reverse phase column
C 18 Reverse Phase Column, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 92/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/c 18 reverse phase column/product/Agilent technologies
Average 92 stars, based on 35 article reviews
Price from $9.99 to $1999.99
c 18 reverse phase column - by Bioz Stars, 2020-09
92/100 stars

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High Performance Liquid Chromatography:

Article Title: Comprehensive Synthesis of Amino Acid-Derived Thiazole Peptidomimetic Analogues to Understand Enigmatic Drug/Substrate-Binding Site of P-glycoprotein
Article Snippet: .. Target compounds purity analysis was accomplished utilizing Agilent 1100 HPLC system with an autosampler (Agilent, Santa Clara, CA) eluting a C-18 reverse phase column (Agilent Eclipse plus C18, 3.5 μ m, 4.6 × 100 mm) with an isocratic mobile phase flow (1.0 mL/min), and samples monitored under UV light at 254 nm. ..

Article Title: Molecular Imaging of Mesothelioma with 99mTc-ECG and 68Ga-ECG
Article Snippet: .. High-performance liquid chromatography (HPLC), equipped with a NaI detector and UV detector (210 nm), was performed on a C-18 reverse phase column (C18-extend, Agilent, Santa Clara, CA) eluted with acetonitrile/water (1 : 9, V/V) at a flow rate of 0.5 mL/min. .. HPLC of cold 69 Ga-ECG was used to confirm the structure of 68 Ga-ECG.

Article Title: Structure-based Mechanistic Insights into Terminal Amide Synthase in Nosiheptide-Represented Thiopeptides Biosynthesis
Article Snippet: .. To determine whether the product nosiheptide was generated or not, HPLC analysis was performed by running C-18 reverse phase column (Agilent 1100) with a MeCN/H2 O gradient mobile phase. ..

Article Title: Reaction Kinetics for the Biocatalytic Conversion of Phenazine-1-Carboxylic Acid to 2-Hydroxyphenazine
Article Snippet: .. The HPLC analysis method for simultaneous analysis of the phenazine compounds PCA, 2-OH-PCA and 2-OH-PHZ was carried out using a 1260 Infinity HPLC apparatus (Agilent Technologies Group, Beijing, China)equipped with a UV detector and a C-18 reverse phase column (Agilent, USA) as described previously . ..

Article Title: Design and Mechanism of (S)-3-Amino-4-(difluoromethylenyl)cyclopent-1-ene-1-carboxylic Acid, a Highly Potent GABA Aminotransferase Inactivator for the Treatment of Addiction
Article Snippet: .. An Agilent 1260 infinity HPLC system was used, which consists of a variable wavelength detector (G1314A), a thermostatted column compartment (G1316A), an autosampler (G1329B), an evaporative light scattering detector (ELSD, G4261A), a quaternary pump (G1311B), and a C-18 reverse phase column (Agilent Poroshell 120, 2.7 μm, 4.6 mm × 50 mm). ..

Injection:

Article Title: Proximal tubule apical endocytosis is modulated by fluid shear stress via an mTOR-dependent pathway
Article Snippet: .. In this regard, aliquots of cell extracts (20 μl) were injected into a C-18 reverse-phase column (Agilent Prep-C18 Scalar, 5 μm, 100 × 4.6 mm) protected by a guard cartridge and connected with a fluorescence detector. ..

Article Title: Proximal tubule apical endocytosis is modulated by fluid shear stress via an mTOR-dependent pathway
Article Snippet: .. Aliquots of samples (2 μl) were injected into a C-18 reverse-phase column (Agilent Prep-C18 Scalar, 5 μm, 100 × 4.6 mm) protected by a guard cartridge. ..

Flow Cytometry:

Article Title: Comprehensive Synthesis of Amino Acid-Derived Thiazole Peptidomimetic Analogues to Understand Enigmatic Drug/Substrate-Binding Site of P-glycoprotein
Article Snippet: .. Target compounds purity analysis was accomplished utilizing Agilent 1100 HPLC system with an autosampler (Agilent, Santa Clara, CA) eluting a C-18 reverse phase column (Agilent Eclipse plus C18, 3.5 μ m, 4.6 × 100 mm) with an isocratic mobile phase flow (1.0 mL/min), and samples monitored under UV light at 254 nm. ..

Article Title: Molecular Imaging of Mesothelioma with 99mTc-ECG and 68Ga-ECG
Article Snippet: .. High-performance liquid chromatography (HPLC), equipped with a NaI detector and UV detector (210 nm), was performed on a C-18 reverse phase column (C18-extend, Agilent, Santa Clara, CA) eluted with acetonitrile/water (1 : 9, V/V) at a flow rate of 0.5 mL/min. .. HPLC of cold 69 Ga-ECG was used to confirm the structure of 68 Ga-ECG.

Fluorescence:

Article Title: Proximal tubule apical endocytosis is modulated by fluid shear stress via an mTOR-dependent pathway
Article Snippet: .. In this regard, aliquots of cell extracts (20 μl) were injected into a C-18 reverse-phase column (Agilent Prep-C18 Scalar, 5 μm, 100 × 4.6 mm) protected by a guard cartridge and connected with a fluorescence detector. ..

Generated:

Article Title: Structure-based Mechanistic Insights into Terminal Amide Synthase in Nosiheptide-Represented Thiopeptides Biosynthesis
Article Snippet: .. To determine whether the product nosiheptide was generated or not, HPLC analysis was performed by running C-18 reverse phase column (Agilent 1100) with a MeCN/H2 O gradient mobile phase. ..

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  • 90
    Agilent technologies semi preparative c18 reverse phase column
    Characterisation of the mature R3/I5 mutants. ( A ) Purity analysis of the mature R3/I5 mutants by <t>C18</t> reverse-phase HPLC. ( B ) Structural analysis of the mature R3/I5 mutants by circular dichroism.
    Semi Preparative C18 Reverse Phase Column, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 44 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/semi preparative c18 reverse phase column/product/Agilent technologies
    Average 90 stars, based on 44 article reviews
    Price from $9.99 to $1999.99
    semi preparative c18 reverse phase column - by Bioz Stars, 2020-09
    90/100 stars
      Buy from Supplier

    99
    Agilent technologies zorbax sb c18
    GSH-activatable response of Biotin-ss-CPT. (A) Proposed mechanism in the targeted delivery system (Biotin-ss-CPT) and the CPT activation by GSH. (B) Reverse-phase HPLC chromatograms of Biotin-ss-CPT with GSH. Peaks in the chromatograms were detected by monitoring the UV/Vis absorption at 365 nm. HPLC: Agilent 1260 Infinity II system, Agilent <t>ZORBAX</t> SB <t>C18</t> (Santa Clara, CA, USA) (250*4.6 mm, 5 µm) Mobile phase A: H 2 O, B: CH 3 CN. 0–20 min: 50–100% B. Flow rate: 1 mL/min. (C) HRMS spectrum of the products from the reaction of Biotin-ss-CPT (20 µM) in a mixed solution of PBS buffer and DMSO (v/v: 4/1) with two equivalent of GSH. Spectrum was obtained 12 h after incubation at 37 °C. The pH dependence at 5.3 (D), 6.8 (E) and 7.4 (F) on the fluorescence spectra variation of Biotin-ss-CPT in the presence of GSH (8 equiv) and the variation in fluorescence intensity at 430 nm (G) before and after incubation with GSH. The data were recorded every 30 min for 2 h after incubation with GSH in mixed solution of PBS buffer and DMSO (v/v: 4/1). Excitation was set at 365 nm.
    Zorbax Sb C18, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 63 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/zorbax sb c18/product/Agilent technologies
    Average 99 stars, based on 63 article reviews
    Price from $9.99 to $1999.99
    zorbax sb c18 - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

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    Characterisation of the mature R3/I5 mutants. ( A ) Purity analysis of the mature R3/I5 mutants by C18 reverse-phase HPLC. ( B ) Structural analysis of the mature R3/I5 mutants by circular dichroism.

    Journal: Scientific Reports

    Article Title: Development of a selective agonist for relaxin family peptide receptor 3

    doi: 10.1038/s41598-017-03465-7

    Figure Lengend Snippet: Characterisation of the mature R3/I5 mutants. ( A ) Purity analysis of the mature R3/I5 mutants by C18 reverse-phase HPLC. ( B ) Structural analysis of the mature R3/I5 mutants by circular dichroism.

    Article Snippet: Refolded precursors were purified by HPLC using a semi-preparative C18 reverse-phase column (Zorbax 300SB-C18, 9.4 × 250 mm, Agilent Technology, Santa Clara, CA, USA), and then sequentially treated with endoproteinase Lys-C, papaya glutaminyl cyclase, and carboxypeptidase B, as described in our previous studies , .

    Techniques: High Performance Liquid Chromatography

    HPLC chromatograms of 16 reference standards separated with different Agilent RPLC columns with methanol and 0.1% formic acid as the mobile phase (254 nm). (A) Zorbax SB-C8 (4.6 mm × 150 mm, 5.0 μm); (B) Zorbax SB-C18 (2.1 mm × 100 mm, 3.5 μm); (C) Eclipse plus C18 (2.1 mm × 100 mm, 3.5 μm); (D) Eclipse plus C18 (4.6 mm × 100 mm, 3.5 μm).

    Journal: Frontiers in Pharmacology

    Article Title: Chemical Profiling and Comparison of Sangju Ganmao Tablet and Its Component Herbs Using Two-Dimensional Liquid Chromatography to Explore Compatibility Mechanism of Herbs

    doi: 10.3389/fphar.2018.01167

    Figure Lengend Snippet: HPLC chromatograms of 16 reference standards separated with different Agilent RPLC columns with methanol and 0.1% formic acid as the mobile phase (254 nm). (A) Zorbax SB-C8 (4.6 mm × 150 mm, 5.0 μm); (B) Zorbax SB-C18 (2.1 mm × 100 mm, 3.5 μm); (C) Eclipse plus C18 (2.1 mm × 100 mm, 3.5 μm); (D) Eclipse plus C18 (4.6 mm × 100 mm, 3.5 μm).

    Article Snippet: For RPLC, Agilent Zorbax SB-C8 (4.6 mm × 150 mm, 5.0 μm), Agilent Zorbax SB-C18 (2.1 mm × 100 mm, 3.5 μm), Agilent Eclipse plus C18 (2.1 mm × 100 mm, 3.5 μm) and Agilent Eclipse plus C18 (4.6 mm × 100 mm, 3.5 μm) columns were studied.

    Techniques: High Performance Liquid Chromatography

    GSH-activatable response of Biotin-ss-CPT. (A) Proposed mechanism in the targeted delivery system (Biotin-ss-CPT) and the CPT activation by GSH. (B) Reverse-phase HPLC chromatograms of Biotin-ss-CPT with GSH. Peaks in the chromatograms were detected by monitoring the UV/Vis absorption at 365 nm. HPLC: Agilent 1260 Infinity II system, Agilent ZORBAX SB C18 (Santa Clara, CA, USA) (250*4.6 mm, 5 µm) Mobile phase A: H 2 O, B: CH 3 CN. 0–20 min: 50–100% B. Flow rate: 1 mL/min. (C) HRMS spectrum of the products from the reaction of Biotin-ss-CPT (20 µM) in a mixed solution of PBS buffer and DMSO (v/v: 4/1) with two equivalent of GSH. Spectrum was obtained 12 h after incubation at 37 °C. The pH dependence at 5.3 (D), 6.8 (E) and 7.4 (F) on the fluorescence spectra variation of Biotin-ss-CPT in the presence of GSH (8 equiv) and the variation in fluorescence intensity at 430 nm (G) before and after incubation with GSH. The data were recorded every 30 min for 2 h after incubation with GSH in mixed solution of PBS buffer and DMSO (v/v: 4/1). Excitation was set at 365 nm.

    Journal: Drug Delivery

    Article Title: Cancer-targeted design of bioresponsive prodrug with enhanced cellular uptake to achieve precise cancer therapy

    doi: 10.1080/10717544.2018.1477862

    Figure Lengend Snippet: GSH-activatable response of Biotin-ss-CPT. (A) Proposed mechanism in the targeted delivery system (Biotin-ss-CPT) and the CPT activation by GSH. (B) Reverse-phase HPLC chromatograms of Biotin-ss-CPT with GSH. Peaks in the chromatograms were detected by monitoring the UV/Vis absorption at 365 nm. HPLC: Agilent 1260 Infinity II system, Agilent ZORBAX SB C18 (Santa Clara, CA, USA) (250*4.6 mm, 5 µm) Mobile phase A: H 2 O, B: CH 3 CN. 0–20 min: 50–100% B. Flow rate: 1 mL/min. (C) HRMS spectrum of the products from the reaction of Biotin-ss-CPT (20 µM) in a mixed solution of PBS buffer and DMSO (v/v: 4/1) with two equivalent of GSH. Spectrum was obtained 12 h after incubation at 37 °C. The pH dependence at 5.3 (D), 6.8 (E) and 7.4 (F) on the fluorescence spectra variation of Biotin-ss-CPT in the presence of GSH (8 equiv) and the variation in fluorescence intensity at 430 nm (G) before and after incubation with GSH. The data were recorded every 30 min for 2 h after incubation with GSH in mixed solution of PBS buffer and DMSO (v/v: 4/1). Excitation was set at 365 nm.

    Article Snippet: HPLC: Agilent 1260 infinity II system, Agilent ZORBAX SB C18 (Santa Clara, CA, USA) (250*4.6 mm, 5 µm) Mobile phase A: H2 O, B: CH3 CN.

    Techniques: Cycling Probe Technology, Activation Assay, High Performance Liquid Chromatography, Flow Cytometry, Incubation, Fluorescence