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<t>Bulk</t> <t>RNA-seq</t> of human DPSCs from male and female donors. (A) Principal component analysis of rlog-normalized counts for the top 500 most variable genes with concentration eclipses showing within-group dispersion. Each point represents one donor. (B) Pairwise sample–sample correlation matrix between male (M) and female (F) DPSCs. Colors show Pearson correlation coefficients. (C) Unsupervised hierarchical clustering heatmap of all expressed genes using Euclidean distance and complete linkage. (D) Summary of differentially expressed genes (DEGs) in male DPSCs compared to female DPSCs. Outer circles show totals at adjusted p value < 0.05, and inner circles show the subset with |log 2 FC| > 1. (E) MA plot showing sex-biased gene expression in DPSCs. Statistically significant DEGs (adjusted p < 0.05) are marked in green, blue, and red for autosomal, X-linked, and Y-linked genes, respectively. (F) Volcano plot of RNA-seq data with represented genes labelled.
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Bulk RNA-seq of human DPSCs from male and female donors. (A) Principal component analysis of rlog-normalized counts for the top 500 most variable genes with concentration eclipses showing within-group dispersion. Each point represents one donor. (B) Pairwise sample–sample correlation matrix between male (M) and female (F) DPSCs. Colors show Pearson correlation coefficients. (C) Unsupervised hierarchical clustering heatmap of all expressed genes using Euclidean distance and complete linkage. (D) Summary of differentially expressed genes (DEGs) in male DPSCs compared to female DPSCs. Outer circles show totals at adjusted p value < 0.05, and inner circles show the subset with |log 2 FC| > 1. (E) MA plot showing sex-biased gene expression in DPSCs. Statistically significant DEGs (adjusted p < 0.05) are marked in green, blue, and red for autosomal, X-linked, and Y-linked genes, respectively. (F) Volcano plot of RNA-seq data with represented genes labelled.

Journal: Regenerative Therapy

Article Title: Sex as a biological variable in human dental pulp stem cells: An exploratory epigenomic and transcriptomic comparison

doi: 10.1016/j.reth.2026.101117

Figure Lengend Snippet: Bulk RNA-seq of human DPSCs from male and female donors. (A) Principal component analysis of rlog-normalized counts for the top 500 most variable genes with concentration eclipses showing within-group dispersion. Each point represents one donor. (B) Pairwise sample–sample correlation matrix between male (M) and female (F) DPSCs. Colors show Pearson correlation coefficients. (C) Unsupervised hierarchical clustering heatmap of all expressed genes using Euclidean distance and complete linkage. (D) Summary of differentially expressed genes (DEGs) in male DPSCs compared to female DPSCs. Outer circles show totals at adjusted p value < 0.05, and inner circles show the subset with |log 2 FC| > 1. (E) MA plot showing sex-biased gene expression in DPSCs. Statistically significant DEGs (adjusted p < 0.05) are marked in green, blue, and red for autosomal, X-linked, and Y-linked genes, respectively. (F) Volcano plot of RNA-seq data with represented genes labelled.

Article Snippet: Bulk RNA sequencing (RNA-seq) was performed by Novogene Ltd. (Cambridge, UK).

Techniques: RNA Sequencing, Concentration Assay, Dispersion, Gene Expression