buffers invitrogen t4 ligase  (Thermo Fisher)


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    Structured Review

    Thermo Fisher buffers invitrogen t4 ligase
    Buffers Invitrogen T4 Ligase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/buffers invitrogen t4 ligase/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    buffers invitrogen t4 ligase - by Bioz Stars, 2020-05
    99/100 stars

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    Related Articles

    Nucleic Acid Electrophoresis:

    Article Title: Reverse transcriptase and endonuclease activities encoded by Penelope-like retroelements
    Article Snippet: .. After addition of 0.5 Weiss unit of T4 DNA ligase (Fermentas, Hanover, MD), reactions were incubated at 16°C and analyzed by denaturing gel electrophoresis as above. ..

    Ligation:

    Article Title: Identification of recognition residues for ligation-based detection and quantitation of pseudouridine and N6-methyladenosine
    Article Snippet: .. The optimized ligation reactions were carried out with 0.15 µM 30-mer RNA with or without Ψ or m6 A modifications, 0.5 µM floater and 0.38 µM of 5′-32 P-labeled anchor in 66 mM Tris–HCl, pH 7.6, 0.5 mM ZnCl2 , 10 mM DTT, 66 µM ATP, 15% DMSO and 0.25 U/µl T4 DNA ligase (USB Inc.). .. All components were mixed and incubated at 16°C for 16 h, and the ligation products separated on denaturing polyacrylamide gels containing 7 M urea.

    Article Title: Practical Synthesis of Cap‐4 RNA
    Article Snippet: .. The 38‐nt T. cruzi cap‐4 RNA was prepared by splinted enzymatic ligation of an 11‐nt cap‐4 RNA and a chemically synthesized 5′‐phosphorylated 27‐nt RNA by using T4 DNA ligase (Thermo Fisher) in analogy to ref. . ..

    Article Title: Profiling non-lysyl tRNAs in HIV-1
    Article Snippet: .. The ligation reaction was carried out overnight (∼16 h) at 16°C with 0.13 μg/μL total RNA in 1X T4 DNA ligase buffer, 0.5 U/μL T4 DNA ligase (USB Corporation, 70042X), 15% DMSO, and 4.5 μM labeling oligonucleotide. .. Hybridization was performed at 60°C overnight (∼16 h) with 1–2 μg each of Cy3- or Cy5-labeled total RNA as previously described ( ).

    Synthesized:

    Article Title: Practical Synthesis of Cap‐4 RNA
    Article Snippet: .. The 38‐nt T. cruzi cap‐4 RNA was prepared by splinted enzymatic ligation of an 11‐nt cap‐4 RNA and a chemically synthesized 5′‐phosphorylated 27‐nt RNA by using T4 DNA ligase (Thermo Fisher) in analogy to ref. . ..

    Labeling:

    Article Title: Profiling non-lysyl tRNAs in HIV-1
    Article Snippet: .. The ligation reaction was carried out overnight (∼16 h) at 16°C with 0.13 μg/μL total RNA in 1X T4 DNA ligase buffer, 0.5 U/μL T4 DNA ligase (USB Corporation, 70042X), 15% DMSO, and 4.5 μM labeling oligonucleotide. .. Hybridization was performed at 60°C overnight (∼16 h) with 1–2 μg each of Cy3- or Cy5-labeled total RNA as previously described ( ).

    Incubation:

    Article Title: Autophosphorylation-dependent remodeling of the DNA-dependent protein kinase catalytic subunit regulates ligation of DNA ends
    Article Snippet: .. Reactions were started by the addition of 0.5 U of T4 DNA ligase (Invitrogen, Carlsbad, CA) and incubated at 37°C. ..

    Article Title: Reverse transcriptase and endonuclease activities encoded by Penelope-like retroelements
    Article Snippet: .. After addition of 0.5 Weiss unit of T4 DNA ligase (Fermentas, Hanover, MD), reactions were incubated at 16°C and analyzed by denaturing gel electrophoresis as above. ..

    Mass Spectrometry:

    Article Title: Detection of Ligation Products of DNA Linkers with 5?-OH Ends by Denaturing PAGE Silver Stain
    Article Snippet: .. A quality inspection report of T4 DNA ligase from Fermentas showed that T4 PNK could not be detected in their T4 DNA ligase ( ); (iii) PNK could not be detected in T4 DNA ligase (Fermentas) by using mass spectrometry (MS) analysis ( and ); (iv) PNK is abundant in mammalian cells but absent in E. coli cells . .. Therefore, the endogenous PNK should be absent in the host E. coli cells that carry plasmids enabling T4 or E. coli DNA ligase high expression; (v) The ligation of linkers A–B and E–F could not be significantly inhibited by (NH4 )2 SO4 , a strong inhibitor of T4 PNK ( , and ); and (vi) T4 PNK requires ATP for activity.

    Modification:

    Article Title: In vitro repair of complex unligatable oxidatively induced DNA double-strand breaks by human cell extracts
    Article Snippet: .. Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum, non-essential amino acids (10 mM), glutamine (100 mM), penicillin/streptomycin (10 000 U/ml), Saccharomyces cerevisiae tRNA and T4 DNA ligase (1 U/µl) were purchased from Life Technologies (Gaithersburg, MD). .. Bleomycin and phenylmethylsulfonyl fluoride (PMSF) were obtained from Sigma (St Louis, MO).

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    Thermo Fisher invitrogen t4 ligase
    Invitrogen T4 Ligase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 183 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/invitrogen t4 ligase/product/Thermo Fisher
    Average 95 stars, based on 183 article reviews
    Price from $9.99 to $1999.99
    invitrogen t4 ligase - by Bioz Stars, 2020-05
    95/100 stars
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