buffer b bfa i  (Promega)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Name:
    Buffer A BWA
    Description:
    Component of the Maxwell HT DNA FFPE Isolation System
    Catalog Number:
    a6371
    Price:
    None
    Category:
    Nucleic Acid Extraction Analysis Nucleic Acid Extraction Genomic DNA
    Buy from Supplier


    Structured Review

    Promega buffer b bfa i
    Component of the Maxwell HT DNA FFPE Isolation System
    https://www.bioz.com/result/buffer b bfa i/product/Promega
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    buffer b bfa i - by Bioz Stars, 2020-08
    99/100 stars

    Related Products / Commonly Used Together

    dde i
    xmn
    hsp 92ii
    dde
    nla iv

    Images

    Related Articles

    Amplification:

    Article Title: Bovine Leukemia Virus Structural Gene Vectors Are Immunogenic and Lack Pathogenicity in a Rabbit Model
    Article Snippet: .. One microgram of PBMC total RNA was subjected to reverse transcription for 1 h at 48°C and PCR amplification in the single-buffer Access reverse transcription-PCR (RT-PCR) system (Promega) with BLV pol primers KB2341 and KB3175. .. Nested PCR with 1/50 of the primary PCR product was performed with BLV pol primers KB560 and KB561.

    Polymerase Chain Reaction:

    Article Title: Bovine Leukemia Virus Structural Gene Vectors Are Immunogenic and Lack Pathogenicity in a Rabbit Model
    Article Snippet: .. One microgram of PBMC total RNA was subjected to reverse transcription for 1 h at 48°C and PCR amplification in the single-buffer Access reverse transcription-PCR (RT-PCR) system (Promega) with BLV pol primers KB2341 and KB3175. .. Nested PCR with 1/50 of the primary PCR product was performed with BLV pol primers KB560 and KB561.

    Article Title: Genomic organization and promoter analysis of the human heat shock factor 2 gene
    Article Snippet: .. The PCR reaction was carried out under the following conditions: primers (300 nM); 0.2–0.3 μg genomic P1 plasmid; 1X Pfu-PCR buffer containing MgCl2 (1.5 mM, Promega); dATP, dGTP, dCTP, and dTTP (0.2 mM each); and Pfu DNA polymerase (1.25 U, Promega) in a volume of 50 μL. ..

    Protease Inhibitor:

    Article Title: The roles of RRP15 in nucleolar formation, ribosome biogenesis and checkpoint control in human cells
    Article Snippet: .. The nuclear lysate was extracted in extraction buffer (25 mM Tris (pH 7.5), 100 mM KCl, 1 mM DTT, 2 mM EDTA, 0.1% NP-40, 1 mM NaF, 40 mg/ml of phenylmethylsulfonyl fluoride, 10 mg/ml of protease inhibitor cocktail and 0.1U/ml of RNasin (Promega)) and sonicated. .. The nuclear lysate was overlaid on 10 to 30% (wt/wt) sucrose gradients in preribosome buffer (25 mM Tris (pH 7.5), 100 mM KCl, 1 mM DTT and 2 mM EDTA) and centrifuged at 36,000 rpm for 3 h at 4°C in a Beckman SW41Ti rotor.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Bovine Leukemia Virus Structural Gene Vectors Are Immunogenic and Lack Pathogenicity in a Rabbit Model
    Article Snippet: .. One microgram of PBMC total RNA was subjected to reverse transcription for 1 h at 48°C and PCR amplification in the single-buffer Access reverse transcription-PCR (RT-PCR) system (Promega) with BLV pol primers KB2341 and KB3175. .. Nested PCR with 1/50 of the primary PCR product was performed with BLV pol primers KB560 and KB561.

    Sequencing:

    Article Title: Aspergillus niger membrane-associated proteome analysis for the identification of glucose transporters
    Article Snippet: .. Approximately 150 μL of digestion buffer, containing sequencing grade modified trypsin (12.5 ng μL−1 ) (Promega, Madison, WI, USA) in ABC, was added to each sample, making sure that all gel pieces were kept wet during digestion (adding, if necessary, additional ABC solution). .. Peptide digestion products were extracted by adding 50 μL of 2 % trifluoroacetic acid (TFA), followed by an incubation step in a thermoblock (ThermoMixer) for 20 min, at room temperature and vigorous stirring (1400 rpm).

    Isolation:

    Article Title: Mapping targets for small nucleolar RNAs in yeast
    Article Snippet: .. RNA-protein complexes were isolated by binding to an IgG column (GE), washed in buffer A and released by TEV (Promega) cleavage. .. RNAs were partially digested using RNaceIT Ribonuclease Cocktail (Agilent).

    Modification:

    Article Title: Aspergillus niger membrane-associated proteome analysis for the identification of glucose transporters
    Article Snippet: .. Approximately 150 μL of digestion buffer, containing sequencing grade modified trypsin (12.5 ng μL−1 ) (Promega, Madison, WI, USA) in ABC, was added to each sample, making sure that all gel pieces were kept wet during digestion (adding, if necessary, additional ABC solution). .. Peptide digestion products were extracted by adding 50 μL of 2 % trifluoroacetic acid (TFA), followed by an incubation step in a thermoblock (ThermoMixer) for 20 min, at room temperature and vigorous stirring (1400 rpm).

    Sonication:

    Article Title: The roles of RRP15 in nucleolar formation, ribosome biogenesis and checkpoint control in human cells
    Article Snippet: .. The nuclear lysate was extracted in extraction buffer (25 mM Tris (pH 7.5), 100 mM KCl, 1 mM DTT, 2 mM EDTA, 0.1% NP-40, 1 mM NaF, 40 mg/ml of phenylmethylsulfonyl fluoride, 10 mg/ml of protease inhibitor cocktail and 0.1U/ml of RNasin (Promega)) and sonicated. .. The nuclear lysate was overlaid on 10 to 30% (wt/wt) sucrose gradients in preribosome buffer (25 mM Tris (pH 7.5), 100 mM KCl, 1 mM DTT and 2 mM EDTA) and centrifuged at 36,000 rpm for 3 h at 4°C in a Beckman SW41Ti rotor.

    Binding Assay:

    Article Title: Mapping targets for small nucleolar RNAs in yeast
    Article Snippet: .. RNA-protein complexes were isolated by binding to an IgG column (GE), washed in buffer A and released by TEV (Promega) cleavage. .. RNAs were partially digested using RNaceIT Ribonuclease Cocktail (Agilent).

    SDS Page:

    Article Title: A novel mechanism of RNase L inhibition: Theiler's virus L* protein prevents 2-5A from binding to RNase L
    Article Snippet: .. The peak fractions containing L* were pooled and diluted 1:3 in buffer A and bound with Ni-NTA resin, washed with buffer A containing 10 mM imidazole and eluted with 150 mM imidazole in buffer A. Fractions were loaded on 12% SDS-PAGE and stained with Gel-Code Blue (Promega). .. Human RNase L was produced as described [ ].

    Plasmid Preparation:

    Article Title: Genomic organization and promoter analysis of the human heat shock factor 2 gene
    Article Snippet: .. The PCR reaction was carried out under the following conditions: primers (300 nM); 0.2–0.3 μg genomic P1 plasmid; 1X Pfu-PCR buffer containing MgCl2 (1.5 mM, Promega); dATP, dGTP, dCTP, and dTTP (0.2 mM each); and Pfu DNA polymerase (1.25 U, Promega) in a volume of 50 μL. ..

    Staining:

    Article Title: A novel mechanism of RNase L inhibition: Theiler's virus L* protein prevents 2-5A from binding to RNase L
    Article Snippet: .. The peak fractions containing L* were pooled and diluted 1:3 in buffer A and bound with Ni-NTA resin, washed with buffer A containing 10 mM imidazole and eluted with 150 mM imidazole in buffer A. Fractions were loaded on 12% SDS-PAGE and stained with Gel-Code Blue (Promega). .. Human RNase L was produced as described [ ].

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Promega buffer b bfa i
    Buffer B Bfa I, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/buffer b bfa i/product/Promega
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    buffer b bfa i - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    Image Search Results