btg2  (Abcam)

 
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    Name:
    Recombinant Human BTG2 protein
    Description:

    Catalog Number:
    AB132314
    Price:
    None
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    Structured Review

    Abcam btg2
    Regulation of Btg1 and <t>Btg2</t> mRNA expression by activators or inhibitors of intracellular signaling pathways in granulosa cells in vitro . A and B, Real-time PCR shows mRNA expression for Btg1 (A) and Btg2 (B) in granulosa cells from rat preovulatory ovaries

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    Average 86 stars, based on 1 article reviews
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    btg2 - by Bioz Stars, 2021-04
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    Images

    1) Product Images from "The B Cell Translocation Gene (BTG) Family in the Rat Ovary: Hormonal Induction, Regulation, and Impact on Cell Cycle Kinetics"

    Article Title: The B Cell Translocation Gene (BTG) Family in the Rat Ovary: Hormonal Induction, Regulation, and Impact on Cell Cycle Kinetics

    Journal: Endocrinology

    doi: 10.1210/en.2008-1650

    Regulation of Btg1 and Btg2 mRNA expression by activators or inhibitors of intracellular signaling pathways in granulosa cells in vitro . A and B, Real-time PCR shows mRNA expression for Btg1 (A) and Btg2 (B) in granulosa cells from rat preovulatory ovaries
    Figure Legend Snippet: Regulation of Btg1 and Btg2 mRNA expression by activators or inhibitors of intracellular signaling pathways in granulosa cells in vitro . A and B, Real-time PCR shows mRNA expression for Btg1 (A) and Btg2 (B) in granulosa cells from rat preovulatory ovaries

    Techniques Used: Expressing, In Vitro, Real-time Polymerase Chain Reaction

    Induction of BTG1, BTG2, and BTG3 expression by hCG in rat granulosa cells in vivo . Real-time PCR analysis shows the expression of Btg1 (A), Btg2 (B), and Btg3 (C) mRNA in rat granulosa/luteal cells obtained from PMSG-primed rat preovulatory ovaries collected
    Figure Legend Snippet: Induction of BTG1, BTG2, and BTG3 expression by hCG in rat granulosa cells in vivo . Real-time PCR analysis shows the expression of Btg1 (A), Btg2 (B), and Btg3 (C) mRNA in rat granulosa/luteal cells obtained from PMSG-primed rat preovulatory ovaries collected

    Techniques Used: Expressing, In Vivo, Real-time Polymerase Chain Reaction

    Cellular localization of Btg1 , Btg2 , and Btg3 mRNA in periovulatory rat ovaries. Sections of rat ovaries obtained at 0 h (48 h after PMSG) (panel A), 4 h (panel B), 8 h (panels C, I, and J), 12 h (panel D), or 24 h (panels E–H) after hCG injection
    Figure Legend Snippet: Cellular localization of Btg1 , Btg2 , and Btg3 mRNA in periovulatory rat ovaries. Sections of rat ovaries obtained at 0 h (48 h after PMSG) (panel A), 4 h (panel B), 8 h (panels C, I, and J), 12 h (panel D), or 24 h (panels E–H) after hCG injection

    Techniques Used: Injection

    Stimulation of Btg1 (A) and Btg2 (B) mRNA expression by hCG in rat granulosa cells in vitro . Real-time PCR analysis shows the expression of Btg1 and Btg2 mRNA in granulosa cells obtained from rat preovulatory ovaries (48 h after PMSG) and cultured in
    Figure Legend Snippet: Stimulation of Btg1 (A) and Btg2 (B) mRNA expression by hCG in rat granulosa cells in vitro . Real-time PCR analysis shows the expression of Btg1 and Btg2 mRNA in granulosa cells obtained from rat preovulatory ovaries (48 h after PMSG) and cultured in

    Techniques Used: Expressing, In Vitro, Real-time Polymerase Chain Reaction, Cell Culture

    Cell cycle analysis of granulosa cells after overexpression of rat BTG1 and BTG2. A, Western blot shows that both BTG1 and BTG2 are highly expressed in granulosa cells that were infected with the Ad-BTG1 or Ad-BTG2 adenovirus vector for 48 h. B and C,
    Figure Legend Snippet: Cell cycle analysis of granulosa cells after overexpression of rat BTG1 and BTG2. A, Western blot shows that both BTG1 and BTG2 are highly expressed in granulosa cells that were infected with the Ad-BTG1 or Ad-BTG2 adenovirus vector for 48 h. B and C,

    Techniques Used: Cell Cycle Assay, Over Expression, Western Blot, Infection, Plasmid Preparation

    Stimulation of BTG1, BTG2, and BTG3 expression by hCG in rat ovary. Real-time PCR analysis shows the expression of Btg1 (A), Btg2 (B), and Btg3 (C) mRNA in preovulatory ovaries after hCG administration. Rats were injected with PMSG for 48 h and treated
    Figure Legend Snippet: Stimulation of BTG1, BTG2, and BTG3 expression by hCG in rat ovary. Real-time PCR analysis shows the expression of Btg1 (A), Btg2 (B), and Btg3 (C) mRNA in preovulatory ovaries after hCG administration. Rats were injected with PMSG for 48 h and treated

    Techniques Used: Expressing, Real-time Polymerase Chain Reaction, Injection

    2) Product Images from "B-cell Translocation Gene 2 (BTG2) Stimulates Cellular Antioxidant Defenses through the Antioxidant Transcription Factor NFE2L2 in Human Mammary Epithelial Cells *"

    Article Title: B-cell Translocation Gene 2 (BTG2) Stimulates Cellular Antioxidant Defenses through the Antioxidant Transcription Factor NFE2L2 in Human Mammary Epithelial Cells *

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.M112.367433

    BTG2 protects against oxidative stress. A and B , MCF-7 cells were transfected overnight with wtBTG2 or empty pcDNA3 vector; harvested using trypsin; seeded into 96-well dishes; allowed to attach; exposed to different concentrations of H 2 O 2 for 24 h; and
    Figure Legend Snippet: BTG2 protects against oxidative stress. A and B , MCF-7 cells were transfected overnight with wtBTG2 or empty pcDNA3 vector; harvested using trypsin; seeded into 96-well dishes; allowed to attach; exposed to different concentrations of H 2 O 2 for 24 h; and

    Techniques Used: Transfection, Plasmid Preparation

    BTG2 regulates NFE2L2/ARE signaling. MCF-7 cells were co-transfected with NQO1-ARE-Luc reporter and the indicated expression vector(s); postincubated for 24 h; and harvested for luciferase assays. The total transfected DNA content was kept constant by
    Figure Legend Snippet: BTG2 regulates NFE2L2/ARE signaling. MCF-7 cells were co-transfected with NQO1-ARE-Luc reporter and the indicated expression vector(s); postincubated for 24 h; and harvested for luciferase assays. The total transfected DNA content was kept constant by

    Techniques Used: Transfection, Expressing, Plasmid Preparation, Luciferase

    BTG2 physically associates with NFE2L2. A and B , MCF-7 cells were transfected with FLAG-BTG2 or empty pCMV6-FLAG vector; postincubated for 24 h; and subjected to anti-FLAG ( A ) or anti-NFE2L2 ( B ) IP. Precipitated proteins were Western blotted using anti-FLAG
    Figure Legend Snippet: BTG2 physically associates with NFE2L2. A and B , MCF-7 cells were transfected with FLAG-BTG2 or empty pCMV6-FLAG vector; postincubated for 24 h; and subjected to anti-FLAG ( A ) or anti-NFE2L2 ( B ) IP. Precipitated proteins were Western blotted using anti-FLAG

    Techniques Used: Transfection, Plasmid Preparation, Western Blot

    BTG2 up-regulates expression of antioxidant enzymes. A and B , MCF-7 cells were transfected overnight with wtBTG2 or pcDNA3 vector; postincubated for 24 h to allow gene expression; harvested; and subjected to semiquantitative RT-PCR ( A ) or Western blot
    Figure Legend Snippet: BTG2 up-regulates expression of antioxidant enzymes. A and B , MCF-7 cells were transfected overnight with wtBTG2 or pcDNA3 vector; postincubated for 24 h to allow gene expression; harvested; and subjected to semiquantitative RT-PCR ( A ) or Western blot

    Techniques Used: Expressing, Transfection, Plasmid Preparation, Reverse Transcription Polymerase Chain Reaction, Western Blot

    BTG2 induces growth arrest of H 2 O 2 -treated cells. A , MCF-7 cells were transfected with wtBTG2 or pcDNA3 vector and treated with different concentrations of H 2 O 2 for 24 h, with exposure to BrdU during the last 12 h of H 2 O 2 treatment. The percentage of
    Figure Legend Snippet: BTG2 induces growth arrest of H 2 O 2 -treated cells. A , MCF-7 cells were transfected with wtBTG2 or pcDNA3 vector and treated with different concentrations of H 2 O 2 for 24 h, with exposure to BrdU during the last 12 h of H 2 O 2 treatment. The percentage of

    Techniques Used: Transfection, Plasmid Preparation

    Box B of BTG2 is required for association of BTG2 with NFE2L2 and for BTG2-mediated protection against oxidative stress in MCF-7 cells. A , schematic diagrams showing the structures of the proteins encoded by the wt-HA-BTG2, HA-BTG2-ΔA, HA-BTG2-ΔB,
    Figure Legend Snippet: Box B of BTG2 is required for association of BTG2 with NFE2L2 and for BTG2-mediated protection against oxidative stress in MCF-7 cells. A , schematic diagrams showing the structures of the proteins encoded by the wt-HA-BTG2, HA-BTG2-ΔA, HA-BTG2-ΔB,

    Techniques Used:

    BTG2 and NFE2L2 are present at the ARE. A , untransfected MCF-7 cells were subjected to ChIP assays using anti-NFE2L2 antibody or nonimmune rabbit IgG (negative control). The PCR primers correspond to two different AREs (E1 and E2) of the HO-1 gene, HO-1
    Figure Legend Snippet: BTG2 and NFE2L2 are present at the ARE. A , untransfected MCF-7 cells were subjected to ChIP assays using anti-NFE2L2 antibody or nonimmune rabbit IgG (negative control). The PCR primers correspond to two different AREs (E1 and E2) of the HO-1 gene, HO-1

    Techniques Used: Chromatin Immunoprecipitation, Negative Control, Polymerase Chain Reaction

    BTG2 regulates antioxidant enzyme activity and cellular redox state. A and B , MCF-7 cells were transfected overnight with wtBTG2 or pcDNA3 vector; postincubated for 24 h; and assayed to determine catalase ( A ) or total SOD ( B ) activity. C and D , 184A1
    Figure Legend Snippet: BTG2 regulates antioxidant enzyme activity and cellular redox state. A and B , MCF-7 cells were transfected overnight with wtBTG2 or pcDNA3 vector; postincubated for 24 h; and assayed to determine catalase ( A ) or total SOD ( B ) activity. C and D , 184A1

    Techniques Used: Activity Assay, Transfection, Plasmid Preparation

    3) Product Images from "The B Cell Translocation Gene (BTG) Family in the Rat Ovary: Hormonal Induction, Regulation, and Impact on Cell Cycle Kinetics"

    Article Title: The B Cell Translocation Gene (BTG) Family in the Rat Ovary: Hormonal Induction, Regulation, and Impact on Cell Cycle Kinetics

    Journal: Endocrinology

    doi: 10.1210/en.2008-1650

    Regulation of Btg1 and Btg2 mRNA expression by activators or inhibitors of intracellular signaling pathways in granulosa cells in vitro . A and B, Real-time PCR shows mRNA expression for Btg1 (A) and Btg2 (B) in granulosa cells from rat preovulatory ovaries
    Figure Legend Snippet: Regulation of Btg1 and Btg2 mRNA expression by activators or inhibitors of intracellular signaling pathways in granulosa cells in vitro . A and B, Real-time PCR shows mRNA expression for Btg1 (A) and Btg2 (B) in granulosa cells from rat preovulatory ovaries

    Techniques Used: Expressing, In Vitro, Real-time Polymerase Chain Reaction

    Induction of BTG1, BTG2, and BTG3 expression by hCG in rat granulosa cells in vivo . Real-time PCR analysis shows the expression of Btg1 (A), Btg2 (B), and Btg3 (C) mRNA in rat granulosa/luteal cells obtained from PMSG-primed rat preovulatory ovaries collected
    Figure Legend Snippet: Induction of BTG1, BTG2, and BTG3 expression by hCG in rat granulosa cells in vivo . Real-time PCR analysis shows the expression of Btg1 (A), Btg2 (B), and Btg3 (C) mRNA in rat granulosa/luteal cells obtained from PMSG-primed rat preovulatory ovaries collected

    Techniques Used: Expressing, In Vivo, Real-time Polymerase Chain Reaction

    Cellular localization of Btg1 , Btg2 , and Btg3 mRNA in periovulatory rat ovaries. Sections of rat ovaries obtained at 0 h (48 h after PMSG) (panel A), 4 h (panel B), 8 h (panels C, I, and J), 12 h (panel D), or 24 h (panels E–H) after hCG injection
    Figure Legend Snippet: Cellular localization of Btg1 , Btg2 , and Btg3 mRNA in periovulatory rat ovaries. Sections of rat ovaries obtained at 0 h (48 h after PMSG) (panel A), 4 h (panel B), 8 h (panels C, I, and J), 12 h (panel D), or 24 h (panels E–H) after hCG injection

    Techniques Used: Injection

    Stimulation of Btg1 (A) and Btg2 (B) mRNA expression by hCG in rat granulosa cells in vitro . Real-time PCR analysis shows the expression of Btg1 and Btg2 mRNA in granulosa cells obtained from rat preovulatory ovaries (48 h after PMSG) and cultured in
    Figure Legend Snippet: Stimulation of Btg1 (A) and Btg2 (B) mRNA expression by hCG in rat granulosa cells in vitro . Real-time PCR analysis shows the expression of Btg1 and Btg2 mRNA in granulosa cells obtained from rat preovulatory ovaries (48 h after PMSG) and cultured in

    Techniques Used: Expressing, In Vitro, Real-time Polymerase Chain Reaction, Cell Culture

    Cell cycle analysis of granulosa cells after overexpression of rat BTG1 and BTG2. A, Western blot shows that both BTG1 and BTG2 are highly expressed in granulosa cells that were infected with the Ad-BTG1 or Ad-BTG2 adenovirus vector for 48 h. B and C,
    Figure Legend Snippet: Cell cycle analysis of granulosa cells after overexpression of rat BTG1 and BTG2. A, Western blot shows that both BTG1 and BTG2 are highly expressed in granulosa cells that were infected with the Ad-BTG1 or Ad-BTG2 adenovirus vector for 48 h. B and C,

    Techniques Used: Cell Cycle Assay, Over Expression, Western Blot, Infection, Plasmid Preparation

    Stimulation of BTG1, BTG2, and BTG3 expression by hCG in rat ovary. Real-time PCR analysis shows the expression of Btg1 (A), Btg2 (B), and Btg3 (C) mRNA in preovulatory ovaries after hCG administration. Rats were injected with PMSG for 48 h and treated
    Figure Legend Snippet: Stimulation of BTG1, BTG2, and BTG3 expression by hCG in rat ovary. Real-time PCR analysis shows the expression of Btg1 (A), Btg2 (B), and Btg3 (C) mRNA in preovulatory ovaries after hCG administration. Rats were injected with PMSG for 48 h and treated

    Techniques Used: Expressing, Real-time Polymerase Chain Reaction, Injection

    4) Product Images from "The B Cell Translocation Gene (BTG) Family in the Rat Ovary: Hormonal Induction, Regulation, and Impact on Cell Cycle Kinetics"

    Article Title: The B Cell Translocation Gene (BTG) Family in the Rat Ovary: Hormonal Induction, Regulation, and Impact on Cell Cycle Kinetics

    Journal: Endocrinology

    doi: 10.1210/en.2008-1650

    Regulation of Btg1 and Btg2 mRNA expression by activators or inhibitors of intracellular signaling pathways in granulosa cells in vitro . A and B, Real-time PCR shows mRNA expression for Btg1 (A) and Btg2 (B) in granulosa cells from rat preovulatory ovaries
    Figure Legend Snippet: Regulation of Btg1 and Btg2 mRNA expression by activators or inhibitors of intracellular signaling pathways in granulosa cells in vitro . A and B, Real-time PCR shows mRNA expression for Btg1 (A) and Btg2 (B) in granulosa cells from rat preovulatory ovaries

    Techniques Used: Expressing, In Vitro, Real-time Polymerase Chain Reaction

    Induction of BTG1, BTG2, and BTG3 expression by hCG in rat granulosa cells in vivo . Real-time PCR analysis shows the expression of Btg1 (A), Btg2 (B), and Btg3 (C) mRNA in rat granulosa/luteal cells obtained from PMSG-primed rat preovulatory ovaries collected
    Figure Legend Snippet: Induction of BTG1, BTG2, and BTG3 expression by hCG in rat granulosa cells in vivo . Real-time PCR analysis shows the expression of Btg1 (A), Btg2 (B), and Btg3 (C) mRNA in rat granulosa/luteal cells obtained from PMSG-primed rat preovulatory ovaries collected

    Techniques Used: Expressing, In Vivo, Real-time Polymerase Chain Reaction

    Cellular localization of Btg1 , Btg2 , and Btg3 mRNA in periovulatory rat ovaries. Sections of rat ovaries obtained at 0 h (48 h after PMSG) (panel A), 4 h (panel B), 8 h (panels C, I, and J), 12 h (panel D), or 24 h (panels E–H) after hCG injection
    Figure Legend Snippet: Cellular localization of Btg1 , Btg2 , and Btg3 mRNA in periovulatory rat ovaries. Sections of rat ovaries obtained at 0 h (48 h after PMSG) (panel A), 4 h (panel B), 8 h (panels C, I, and J), 12 h (panel D), or 24 h (panels E–H) after hCG injection

    Techniques Used: Injection

    Stimulation of Btg1 (A) and Btg2 (B) mRNA expression by hCG in rat granulosa cells in vitro . Real-time PCR analysis shows the expression of Btg1 and Btg2 mRNA in granulosa cells obtained from rat preovulatory ovaries (48 h after PMSG) and cultured in
    Figure Legend Snippet: Stimulation of Btg1 (A) and Btg2 (B) mRNA expression by hCG in rat granulosa cells in vitro . Real-time PCR analysis shows the expression of Btg1 and Btg2 mRNA in granulosa cells obtained from rat preovulatory ovaries (48 h after PMSG) and cultured in

    Techniques Used: Expressing, In Vitro, Real-time Polymerase Chain Reaction, Cell Culture

    Cell cycle analysis of granulosa cells after overexpression of rat BTG1 and BTG2. A, Western blot shows that both BTG1 and BTG2 are highly expressed in granulosa cells that were infected with the Ad-BTG1 or Ad-BTG2 adenovirus vector for 48 h. B and C,
    Figure Legend Snippet: Cell cycle analysis of granulosa cells after overexpression of rat BTG1 and BTG2. A, Western blot shows that both BTG1 and BTG2 are highly expressed in granulosa cells that were infected with the Ad-BTG1 or Ad-BTG2 adenovirus vector for 48 h. B and C,

    Techniques Used: Cell Cycle Assay, Over Expression, Western Blot, Infection, Plasmid Preparation

    Stimulation of BTG1, BTG2, and BTG3 expression by hCG in rat ovary. Real-time PCR analysis shows the expression of Btg1 (A), Btg2 (B), and Btg3 (C) mRNA in preovulatory ovaries after hCG administration. Rats were injected with PMSG for 48 h and treated
    Figure Legend Snippet: Stimulation of BTG1, BTG2, and BTG3 expression by hCG in rat ovary. Real-time PCR analysis shows the expression of Btg1 (A), Btg2 (B), and Btg3 (C) mRNA in preovulatory ovaries after hCG administration. Rats were injected with PMSG for 48 h and treated

    Techniques Used: Expressing, Real-time Polymerase Chain Reaction, Injection

    5) Product Images from "The B Cell Translocation Gene (BTG) Family in the Rat Ovary: Hormonal Induction, Regulation, and Impact on Cell Cycle Kinetics"

    Article Title: The B Cell Translocation Gene (BTG) Family in the Rat Ovary: Hormonal Induction, Regulation, and Impact on Cell Cycle Kinetics

    Journal: Endocrinology

    doi: 10.1210/en.2008-1650

    Regulation of Btg1 and Btg2 mRNA expression by activators or inhibitors of intracellular signaling pathways in granulosa cells in vitro . A and B, Real-time PCR shows mRNA expression for Btg1 (A) and Btg2 (B) in granulosa cells from rat preovulatory ovaries
    Figure Legend Snippet: Regulation of Btg1 and Btg2 mRNA expression by activators or inhibitors of intracellular signaling pathways in granulosa cells in vitro . A and B, Real-time PCR shows mRNA expression for Btg1 (A) and Btg2 (B) in granulosa cells from rat preovulatory ovaries

    Techniques Used: Expressing, In Vitro, Real-time Polymerase Chain Reaction

    Induction of BTG1, BTG2, and BTG3 expression by hCG in rat granulosa cells in vivo . Real-time PCR analysis shows the expression of Btg1 (A), Btg2 (B), and Btg3 (C) mRNA in rat granulosa/luteal cells obtained from PMSG-primed rat preovulatory ovaries collected
    Figure Legend Snippet: Induction of BTG1, BTG2, and BTG3 expression by hCG in rat granulosa cells in vivo . Real-time PCR analysis shows the expression of Btg1 (A), Btg2 (B), and Btg3 (C) mRNA in rat granulosa/luteal cells obtained from PMSG-primed rat preovulatory ovaries collected

    Techniques Used: Expressing, In Vivo, Real-time Polymerase Chain Reaction

    Cellular localization of Btg1 , Btg2 , and Btg3 mRNA in periovulatory rat ovaries. Sections of rat ovaries obtained at 0 h (48 h after PMSG) (panel A), 4 h (panel B), 8 h (panels C, I, and J), 12 h (panel D), or 24 h (panels E–H) after hCG injection
    Figure Legend Snippet: Cellular localization of Btg1 , Btg2 , and Btg3 mRNA in periovulatory rat ovaries. Sections of rat ovaries obtained at 0 h (48 h after PMSG) (panel A), 4 h (panel B), 8 h (panels C, I, and J), 12 h (panel D), or 24 h (panels E–H) after hCG injection

    Techniques Used: Injection

    Stimulation of Btg1 (A) and Btg2 (B) mRNA expression by hCG in rat granulosa cells in vitro . Real-time PCR analysis shows the expression of Btg1 and Btg2 mRNA in granulosa cells obtained from rat preovulatory ovaries (48 h after PMSG) and cultured in
    Figure Legend Snippet: Stimulation of Btg1 (A) and Btg2 (B) mRNA expression by hCG in rat granulosa cells in vitro . Real-time PCR analysis shows the expression of Btg1 and Btg2 mRNA in granulosa cells obtained from rat preovulatory ovaries (48 h after PMSG) and cultured in

    Techniques Used: Expressing, In Vitro, Real-time Polymerase Chain Reaction, Cell Culture

    Cell cycle analysis of granulosa cells after overexpression of rat BTG1 and BTG2. A, Western blot shows that both BTG1 and BTG2 are highly expressed in granulosa cells that were infected with the Ad-BTG1 or Ad-BTG2 adenovirus vector for 48 h. B and C,
    Figure Legend Snippet: Cell cycle analysis of granulosa cells after overexpression of rat BTG1 and BTG2. A, Western blot shows that both BTG1 and BTG2 are highly expressed in granulosa cells that were infected with the Ad-BTG1 or Ad-BTG2 adenovirus vector for 48 h. B and C,

    Techniques Used: Cell Cycle Assay, Over Expression, Western Blot, Infection, Plasmid Preparation

    Stimulation of BTG1, BTG2, and BTG3 expression by hCG in rat ovary. Real-time PCR analysis shows the expression of Btg1 (A), Btg2 (B), and Btg3 (C) mRNA in preovulatory ovaries after hCG administration. Rats were injected with PMSG for 48 h and treated
    Figure Legend Snippet: Stimulation of BTG1, BTG2, and BTG3 expression by hCG in rat ovary. Real-time PCR analysis shows the expression of Btg1 (A), Btg2 (B), and Btg3 (C) mRNA in preovulatory ovaries after hCG administration. Rats were injected with PMSG for 48 h and treated

    Techniques Used: Expressing, Real-time Polymerase Chain Reaction, Injection

    6) Product Images from "The B Cell Translocation Gene (BTG) Family in the Rat Ovary: Hormonal Induction, Regulation, and Impact on Cell Cycle Kinetics"

    Article Title: The B Cell Translocation Gene (BTG) Family in the Rat Ovary: Hormonal Induction, Regulation, and Impact on Cell Cycle Kinetics

    Journal: Endocrinology

    doi: 10.1210/en.2008-1650

    Regulation of Btg1 and Btg2 mRNA expression by activators or inhibitors of intracellular signaling pathways in granulosa cells in vitro . A and B, Real-time PCR shows mRNA expression for Btg1 (A) and Btg2 (B) in granulosa cells from rat preovulatory ovaries
    Figure Legend Snippet: Regulation of Btg1 and Btg2 mRNA expression by activators or inhibitors of intracellular signaling pathways in granulosa cells in vitro . A and B, Real-time PCR shows mRNA expression for Btg1 (A) and Btg2 (B) in granulosa cells from rat preovulatory ovaries

    Techniques Used: Expressing, In Vitro, Real-time Polymerase Chain Reaction

    Induction of BTG1, BTG2, and BTG3 expression by hCG in rat granulosa cells in vivo . Real-time PCR analysis shows the expression of Btg1 (A), Btg2 (B), and Btg3 (C) mRNA in rat granulosa/luteal cells obtained from PMSG-primed rat preovulatory ovaries collected
    Figure Legend Snippet: Induction of BTG1, BTG2, and BTG3 expression by hCG in rat granulosa cells in vivo . Real-time PCR analysis shows the expression of Btg1 (A), Btg2 (B), and Btg3 (C) mRNA in rat granulosa/luteal cells obtained from PMSG-primed rat preovulatory ovaries collected

    Techniques Used: Expressing, In Vivo, Real-time Polymerase Chain Reaction

    Cellular localization of Btg1 , Btg2 , and Btg3 mRNA in periovulatory rat ovaries. Sections of rat ovaries obtained at 0 h (48 h after PMSG) (panel A), 4 h (panel B), 8 h (panels C, I, and J), 12 h (panel D), or 24 h (panels E–H) after hCG injection
    Figure Legend Snippet: Cellular localization of Btg1 , Btg2 , and Btg3 mRNA in periovulatory rat ovaries. Sections of rat ovaries obtained at 0 h (48 h after PMSG) (panel A), 4 h (panel B), 8 h (panels C, I, and J), 12 h (panel D), or 24 h (panels E–H) after hCG injection

    Techniques Used: Injection

    Stimulation of Btg1 (A) and Btg2 (B) mRNA expression by hCG in rat granulosa cells in vitro . Real-time PCR analysis shows the expression of Btg1 and Btg2 mRNA in granulosa cells obtained from rat preovulatory ovaries (48 h after PMSG) and cultured in
    Figure Legend Snippet: Stimulation of Btg1 (A) and Btg2 (B) mRNA expression by hCG in rat granulosa cells in vitro . Real-time PCR analysis shows the expression of Btg1 and Btg2 mRNA in granulosa cells obtained from rat preovulatory ovaries (48 h after PMSG) and cultured in

    Techniques Used: Expressing, In Vitro, Real-time Polymerase Chain Reaction, Cell Culture

    Cell cycle analysis of granulosa cells after overexpression of rat BTG1 and BTG2. A, Western blot shows that both BTG1 and BTG2 are highly expressed in granulosa cells that were infected with the Ad-BTG1 or Ad-BTG2 adenovirus vector for 48 h. B and C,
    Figure Legend Snippet: Cell cycle analysis of granulosa cells after overexpression of rat BTG1 and BTG2. A, Western blot shows that both BTG1 and BTG2 are highly expressed in granulosa cells that were infected with the Ad-BTG1 or Ad-BTG2 adenovirus vector for 48 h. B and C,

    Techniques Used: Cell Cycle Assay, Over Expression, Western Blot, Infection, Plasmid Preparation

    Stimulation of BTG1, BTG2, and BTG3 expression by hCG in rat ovary. Real-time PCR analysis shows the expression of Btg1 (A), Btg2 (B), and Btg3 (C) mRNA in preovulatory ovaries after hCG administration. Rats were injected with PMSG for 48 h and treated
    Figure Legend Snippet: Stimulation of BTG1, BTG2, and BTG3 expression by hCG in rat ovary. Real-time PCR analysis shows the expression of Btg1 (A), Btg2 (B), and Btg3 (C) mRNA in preovulatory ovaries after hCG administration. Rats were injected with PMSG for 48 h and treated

    Techniques Used: Expressing, Real-time Polymerase Chain Reaction, Injection

    7) Product Images from "B-cell Translocation Gene 2 (BTG2) Stimulates Cellular Antioxidant Defenses through the Antioxidant Transcription Factor NFE2L2 in Human Mammary Epithelial Cells *"

    Article Title: B-cell Translocation Gene 2 (BTG2) Stimulates Cellular Antioxidant Defenses through the Antioxidant Transcription Factor NFE2L2 in Human Mammary Epithelial Cells *

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.M112.367433

    BTG2 protects against oxidative stress. A and B , MCF-7 cells were transfected overnight with wtBTG2 or empty pcDNA3 vector; harvested using trypsin; seeded into 96-well dishes; allowed to attach; exposed to different concentrations of H 2 O 2 for 24 h; and
    Figure Legend Snippet: BTG2 protects against oxidative stress. A and B , MCF-7 cells were transfected overnight with wtBTG2 or empty pcDNA3 vector; harvested using trypsin; seeded into 96-well dishes; allowed to attach; exposed to different concentrations of H 2 O 2 for 24 h; and

    Techniques Used: Transfection, Plasmid Preparation

    BTG2 regulates NFE2L2/ARE signaling. MCF-7 cells were co-transfected with NQO1-ARE-Luc reporter and the indicated expression vector(s); postincubated for 24 h; and harvested for luciferase assays. The total transfected DNA content was kept constant by
    Figure Legend Snippet: BTG2 regulates NFE2L2/ARE signaling. MCF-7 cells were co-transfected with NQO1-ARE-Luc reporter and the indicated expression vector(s); postincubated for 24 h; and harvested for luciferase assays. The total transfected DNA content was kept constant by

    Techniques Used: Transfection, Expressing, Plasmid Preparation, Luciferase

    BTG2 physically associates with NFE2L2. A and B , MCF-7 cells were transfected with FLAG-BTG2 or empty pCMV6-FLAG vector; postincubated for 24 h; and subjected to anti-FLAG ( A ) or anti-NFE2L2 ( B ) IP. Precipitated proteins were Western blotted using anti-FLAG
    Figure Legend Snippet: BTG2 physically associates with NFE2L2. A and B , MCF-7 cells were transfected with FLAG-BTG2 or empty pCMV6-FLAG vector; postincubated for 24 h; and subjected to anti-FLAG ( A ) or anti-NFE2L2 ( B ) IP. Precipitated proteins were Western blotted using anti-FLAG

    Techniques Used: Transfection, Plasmid Preparation, Western Blot

    BTG2 up-regulates expression of antioxidant enzymes. A and B , MCF-7 cells were transfected overnight with wtBTG2 or pcDNA3 vector; postincubated for 24 h to allow gene expression; harvested; and subjected to semiquantitative RT-PCR ( A ) or Western blot
    Figure Legend Snippet: BTG2 up-regulates expression of antioxidant enzymes. A and B , MCF-7 cells were transfected overnight with wtBTG2 or pcDNA3 vector; postincubated for 24 h to allow gene expression; harvested; and subjected to semiquantitative RT-PCR ( A ) or Western blot

    Techniques Used: Expressing, Transfection, Plasmid Preparation, Reverse Transcription Polymerase Chain Reaction, Western Blot

    BTG2 induces growth arrest of H 2 O 2 -treated cells. A , MCF-7 cells were transfected with wtBTG2 or pcDNA3 vector and treated with different concentrations of H 2 O 2 for 24 h, with exposure to BrdU during the last 12 h of H 2 O 2 treatment. The percentage of
    Figure Legend Snippet: BTG2 induces growth arrest of H 2 O 2 -treated cells. A , MCF-7 cells were transfected with wtBTG2 or pcDNA3 vector and treated with different concentrations of H 2 O 2 for 24 h, with exposure to BrdU during the last 12 h of H 2 O 2 treatment. The percentage of

    Techniques Used: Transfection, Plasmid Preparation

    Box B of BTG2 is required for association of BTG2 with NFE2L2 and for BTG2-mediated protection against oxidative stress in MCF-7 cells. A , schematic diagrams showing the structures of the proteins encoded by the wt-HA-BTG2, HA-BTG2-ΔA, HA-BTG2-ΔB,
    Figure Legend Snippet: Box B of BTG2 is required for association of BTG2 with NFE2L2 and for BTG2-mediated protection against oxidative stress in MCF-7 cells. A , schematic diagrams showing the structures of the proteins encoded by the wt-HA-BTG2, HA-BTG2-ΔA, HA-BTG2-ΔB,

    Techniques Used:

    BTG2 and NFE2L2 are present at the ARE. A , untransfected MCF-7 cells were subjected to ChIP assays using anti-NFE2L2 antibody or nonimmune rabbit IgG (negative control). The PCR primers correspond to two different AREs (E1 and E2) of the HO-1 gene, HO-1
    Figure Legend Snippet: BTG2 and NFE2L2 are present at the ARE. A , untransfected MCF-7 cells were subjected to ChIP assays using anti-NFE2L2 antibody or nonimmune rabbit IgG (negative control). The PCR primers correspond to two different AREs (E1 and E2) of the HO-1 gene, HO-1

    Techniques Used: Chromatin Immunoprecipitation, Negative Control, Polymerase Chain Reaction

    BTG2 regulates antioxidant enzyme activity and cellular redox state. A and B , MCF-7 cells were transfected overnight with wtBTG2 or pcDNA3 vector; postincubated for 24 h; and assayed to determine catalase ( A ) or total SOD ( B ) activity. C and D , 184A1
    Figure Legend Snippet: BTG2 regulates antioxidant enzyme activity and cellular redox state. A and B , MCF-7 cells were transfected overnight with wtBTG2 or pcDNA3 vector; postincubated for 24 h; and assayed to determine catalase ( A ) or total SOD ( B ) activity. C and D , 184A1

    Techniques Used: Activity Assay, Transfection, Plasmid Preparation

    8) Product Images from "B-cell Translocation Gene 2 (BTG2) Stimulates Cellular Antioxidant Defenses through the Antioxidant Transcription Factor NFE2L2 in Human Mammary Epithelial Cells *"

    Article Title: B-cell Translocation Gene 2 (BTG2) Stimulates Cellular Antioxidant Defenses through the Antioxidant Transcription Factor NFE2L2 in Human Mammary Epithelial Cells *

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.M112.367433

    BTG2 protects against oxidative stress. A and B , MCF-7 cells were transfected overnight with wtBTG2 or empty pcDNA3 vector; harvested using trypsin; seeded into 96-well dishes; allowed to attach; exposed to different concentrations of H 2 O 2 for 24 h; and
    Figure Legend Snippet: BTG2 protects against oxidative stress. A and B , MCF-7 cells were transfected overnight with wtBTG2 or empty pcDNA3 vector; harvested using trypsin; seeded into 96-well dishes; allowed to attach; exposed to different concentrations of H 2 O 2 for 24 h; and

    Techniques Used: Transfection, Plasmid Preparation

    BTG2 regulates NFE2L2/ARE signaling. MCF-7 cells were co-transfected with NQO1-ARE-Luc reporter and the indicated expression vector(s); postincubated for 24 h; and harvested for luciferase assays. The total transfected DNA content was kept constant by
    Figure Legend Snippet: BTG2 regulates NFE2L2/ARE signaling. MCF-7 cells were co-transfected with NQO1-ARE-Luc reporter and the indicated expression vector(s); postincubated for 24 h; and harvested for luciferase assays. The total transfected DNA content was kept constant by

    Techniques Used: Transfection, Expressing, Plasmid Preparation, Luciferase

    BTG2 physically associates with NFE2L2. A and B , MCF-7 cells were transfected with FLAG-BTG2 or empty pCMV6-FLAG vector; postincubated for 24 h; and subjected to anti-FLAG ( A ) or anti-NFE2L2 ( B ) IP. Precipitated proteins were Western blotted using anti-FLAG
    Figure Legend Snippet: BTG2 physically associates with NFE2L2. A and B , MCF-7 cells were transfected with FLAG-BTG2 or empty pCMV6-FLAG vector; postincubated for 24 h; and subjected to anti-FLAG ( A ) or anti-NFE2L2 ( B ) IP. Precipitated proteins were Western blotted using anti-FLAG

    Techniques Used: Transfection, Plasmid Preparation, Western Blot

    BTG2 up-regulates expression of antioxidant enzymes. A and B , MCF-7 cells were transfected overnight with wtBTG2 or pcDNA3 vector; postincubated for 24 h to allow gene expression; harvested; and subjected to semiquantitative RT-PCR ( A ) or Western blot
    Figure Legend Snippet: BTG2 up-regulates expression of antioxidant enzymes. A and B , MCF-7 cells were transfected overnight with wtBTG2 or pcDNA3 vector; postincubated for 24 h to allow gene expression; harvested; and subjected to semiquantitative RT-PCR ( A ) or Western blot

    Techniques Used: Expressing, Transfection, Plasmid Preparation, Reverse Transcription Polymerase Chain Reaction, Western Blot

    BTG2 induces growth arrest of H 2 O 2 -treated cells. A , MCF-7 cells were transfected with wtBTG2 or pcDNA3 vector and treated with different concentrations of H 2 O 2 for 24 h, with exposure to BrdU during the last 12 h of H 2 O 2 treatment. The percentage of
    Figure Legend Snippet: BTG2 induces growth arrest of H 2 O 2 -treated cells. A , MCF-7 cells were transfected with wtBTG2 or pcDNA3 vector and treated with different concentrations of H 2 O 2 for 24 h, with exposure to BrdU during the last 12 h of H 2 O 2 treatment. The percentage of

    Techniques Used: Transfection, Plasmid Preparation

    Box B of BTG2 is required for association of BTG2 with NFE2L2 and for BTG2-mediated protection against oxidative stress in MCF-7 cells. A , schematic diagrams showing the structures of the proteins encoded by the wt-HA-BTG2, HA-BTG2-ΔA, HA-BTG2-ΔB,
    Figure Legend Snippet: Box B of BTG2 is required for association of BTG2 with NFE2L2 and for BTG2-mediated protection against oxidative stress in MCF-7 cells. A , schematic diagrams showing the structures of the proteins encoded by the wt-HA-BTG2, HA-BTG2-ΔA, HA-BTG2-ΔB,

    Techniques Used:

    BTG2 and NFE2L2 are present at the ARE. A , untransfected MCF-7 cells were subjected to ChIP assays using anti-NFE2L2 antibody or nonimmune rabbit IgG (negative control). The PCR primers correspond to two different AREs (E1 and E2) of the HO-1 gene, HO-1
    Figure Legend Snippet: BTG2 and NFE2L2 are present at the ARE. A , untransfected MCF-7 cells were subjected to ChIP assays using anti-NFE2L2 antibody or nonimmune rabbit IgG (negative control). The PCR primers correspond to two different AREs (E1 and E2) of the HO-1 gene, HO-1

    Techniques Used: Chromatin Immunoprecipitation, Negative Control, Polymerase Chain Reaction

    BTG2 regulates antioxidant enzyme activity and cellular redox state. A and B , MCF-7 cells were transfected overnight with wtBTG2 or pcDNA3 vector; postincubated for 24 h; and assayed to determine catalase ( A ) or total SOD ( B ) activity. C and D , 184A1
    Figure Legend Snippet: BTG2 regulates antioxidant enzyme activity and cellular redox state. A and B , MCF-7 cells were transfected overnight with wtBTG2 or pcDNA3 vector; postincubated for 24 h; and assayed to determine catalase ( A ) or total SOD ( B ) activity. C and D , 184A1

    Techniques Used: Activity Assay, Transfection, Plasmid Preparation

    9) Product Images from "The B Cell Translocation Gene (BTG) Family in the Rat Ovary: Hormonal Induction, Regulation, and Impact on Cell Cycle Kinetics"

    Article Title: The B Cell Translocation Gene (BTG) Family in the Rat Ovary: Hormonal Induction, Regulation, and Impact on Cell Cycle Kinetics

    Journal: Endocrinology

    doi: 10.1210/en.2008-1650

    Regulation of Btg1 and Btg2 mRNA expression by activators or inhibitors of intracellular signaling pathways in granulosa cells in vitro . A and B, Real-time PCR shows mRNA expression for Btg1 (A) and Btg2 (B) in granulosa cells from rat preovulatory ovaries
    Figure Legend Snippet: Regulation of Btg1 and Btg2 mRNA expression by activators or inhibitors of intracellular signaling pathways in granulosa cells in vitro . A and B, Real-time PCR shows mRNA expression for Btg1 (A) and Btg2 (B) in granulosa cells from rat preovulatory ovaries

    Techniques Used: Expressing, In Vitro, Real-time Polymerase Chain Reaction

    Induction of BTG1, BTG2, and BTG3 expression by hCG in rat granulosa cells in vivo . Real-time PCR analysis shows the expression of Btg1 (A), Btg2 (B), and Btg3 (C) mRNA in rat granulosa/luteal cells obtained from PMSG-primed rat preovulatory ovaries collected
    Figure Legend Snippet: Induction of BTG1, BTG2, and BTG3 expression by hCG in rat granulosa cells in vivo . Real-time PCR analysis shows the expression of Btg1 (A), Btg2 (B), and Btg3 (C) mRNA in rat granulosa/luteal cells obtained from PMSG-primed rat preovulatory ovaries collected

    Techniques Used: Expressing, In Vivo, Real-time Polymerase Chain Reaction

    Cellular localization of Btg1 , Btg2 , and Btg3 mRNA in periovulatory rat ovaries. Sections of rat ovaries obtained at 0 h (48 h after PMSG) (panel A), 4 h (panel B), 8 h (panels C, I, and J), 12 h (panel D), or 24 h (panels E–H) after hCG injection
    Figure Legend Snippet: Cellular localization of Btg1 , Btg2 , and Btg3 mRNA in periovulatory rat ovaries. Sections of rat ovaries obtained at 0 h (48 h after PMSG) (panel A), 4 h (panel B), 8 h (panels C, I, and J), 12 h (panel D), or 24 h (panels E–H) after hCG injection

    Techniques Used: Injection

    Stimulation of Btg1 (A) and Btg2 (B) mRNA expression by hCG in rat granulosa cells in vitro . Real-time PCR analysis shows the expression of Btg1 and Btg2 mRNA in granulosa cells obtained from rat preovulatory ovaries (48 h after PMSG) and cultured in
    Figure Legend Snippet: Stimulation of Btg1 (A) and Btg2 (B) mRNA expression by hCG in rat granulosa cells in vitro . Real-time PCR analysis shows the expression of Btg1 and Btg2 mRNA in granulosa cells obtained from rat preovulatory ovaries (48 h after PMSG) and cultured in

    Techniques Used: Expressing, In Vitro, Real-time Polymerase Chain Reaction, Cell Culture

    Cell cycle analysis of granulosa cells after overexpression of rat BTG1 and BTG2. A, Western blot shows that both BTG1 and BTG2 are highly expressed in granulosa cells that were infected with the Ad-BTG1 or Ad-BTG2 adenovirus vector for 48 h. B and C,
    Figure Legend Snippet: Cell cycle analysis of granulosa cells after overexpression of rat BTG1 and BTG2. A, Western blot shows that both BTG1 and BTG2 are highly expressed in granulosa cells that were infected with the Ad-BTG1 or Ad-BTG2 adenovirus vector for 48 h. B and C,

    Techniques Used: Cell Cycle Assay, Over Expression, Western Blot, Infection, Plasmid Preparation

    Stimulation of BTG1, BTG2, and BTG3 expression by hCG in rat ovary. Real-time PCR analysis shows the expression of Btg1 (A), Btg2 (B), and Btg3 (C) mRNA in preovulatory ovaries after hCG administration. Rats were injected with PMSG for 48 h and treated
    Figure Legend Snippet: Stimulation of BTG1, BTG2, and BTG3 expression by hCG in rat ovary. Real-time PCR analysis shows the expression of Btg1 (A), Btg2 (B), and Btg3 (C) mRNA in preovulatory ovaries after hCG administration. Rats were injected with PMSG for 48 h and treated

    Techniques Used: Expressing, Real-time Polymerase Chain Reaction, Injection

    10) Product Images from "The B Cell Translocation Gene (BTG) Family in the Rat Ovary: Hormonal Induction, Regulation, and Impact on Cell Cycle Kinetics"

    Article Title: The B Cell Translocation Gene (BTG) Family in the Rat Ovary: Hormonal Induction, Regulation, and Impact on Cell Cycle Kinetics

    Journal: Endocrinology

    doi: 10.1210/en.2008-1650

    Regulation of Btg1 and Btg2 mRNA expression by activators or inhibitors of intracellular signaling pathways in granulosa cells in vitro . A and B, Real-time PCR shows mRNA expression for Btg1 (A) and Btg2 (B) in granulosa cells from rat preovulatory ovaries
    Figure Legend Snippet: Regulation of Btg1 and Btg2 mRNA expression by activators or inhibitors of intracellular signaling pathways in granulosa cells in vitro . A and B, Real-time PCR shows mRNA expression for Btg1 (A) and Btg2 (B) in granulosa cells from rat preovulatory ovaries

    Techniques Used: Expressing, In Vitro, Real-time Polymerase Chain Reaction

    Induction of BTG1, BTG2, and BTG3 expression by hCG in rat granulosa cells in vivo . Real-time PCR analysis shows the expression of Btg1 (A), Btg2 (B), and Btg3 (C) mRNA in rat granulosa/luteal cells obtained from PMSG-primed rat preovulatory ovaries collected
    Figure Legend Snippet: Induction of BTG1, BTG2, and BTG3 expression by hCG in rat granulosa cells in vivo . Real-time PCR analysis shows the expression of Btg1 (A), Btg2 (B), and Btg3 (C) mRNA in rat granulosa/luteal cells obtained from PMSG-primed rat preovulatory ovaries collected

    Techniques Used: Expressing, In Vivo, Real-time Polymerase Chain Reaction

    Cellular localization of Btg1 , Btg2 , and Btg3 mRNA in periovulatory rat ovaries. Sections of rat ovaries obtained at 0 h (48 h after PMSG) (panel A), 4 h (panel B), 8 h (panels C, I, and J), 12 h (panel D), or 24 h (panels E–H) after hCG injection
    Figure Legend Snippet: Cellular localization of Btg1 , Btg2 , and Btg3 mRNA in periovulatory rat ovaries. Sections of rat ovaries obtained at 0 h (48 h after PMSG) (panel A), 4 h (panel B), 8 h (panels C, I, and J), 12 h (panel D), or 24 h (panels E–H) after hCG injection

    Techniques Used: Injection

    Stimulation of Btg1 (A) and Btg2 (B) mRNA expression by hCG in rat granulosa cells in vitro . Real-time PCR analysis shows the expression of Btg1 and Btg2 mRNA in granulosa cells obtained from rat preovulatory ovaries (48 h after PMSG) and cultured in
    Figure Legend Snippet: Stimulation of Btg1 (A) and Btg2 (B) mRNA expression by hCG in rat granulosa cells in vitro . Real-time PCR analysis shows the expression of Btg1 and Btg2 mRNA in granulosa cells obtained from rat preovulatory ovaries (48 h after PMSG) and cultured in

    Techniques Used: Expressing, In Vitro, Real-time Polymerase Chain Reaction, Cell Culture

    Cell cycle analysis of granulosa cells after overexpression of rat BTG1 and BTG2. A, Western blot shows that both BTG1 and BTG2 are highly expressed in granulosa cells that were infected with the Ad-BTG1 or Ad-BTG2 adenovirus vector for 48 h. B and C,
    Figure Legend Snippet: Cell cycle analysis of granulosa cells after overexpression of rat BTG1 and BTG2. A, Western blot shows that both BTG1 and BTG2 are highly expressed in granulosa cells that were infected with the Ad-BTG1 or Ad-BTG2 adenovirus vector for 48 h. B and C,

    Techniques Used: Cell Cycle Assay, Over Expression, Western Blot, Infection, Plasmid Preparation

    Stimulation of BTG1, BTG2, and BTG3 expression by hCG in rat ovary. Real-time PCR analysis shows the expression of Btg1 (A), Btg2 (B), and Btg3 (C) mRNA in preovulatory ovaries after hCG administration. Rats were injected with PMSG for 48 h and treated
    Figure Legend Snippet: Stimulation of BTG1, BTG2, and BTG3 expression by hCG in rat ovary. Real-time PCR analysis shows the expression of Btg1 (A), Btg2 (B), and Btg3 (C) mRNA in preovulatory ovaries after hCG administration. Rats were injected with PMSG for 48 h and treated

    Techniques Used: Expressing, Real-time Polymerase Chain Reaction, Injection

    11) Product Images from "B-cell Translocation Gene 2 (BTG2) Stimulates Cellular Antioxidant Defenses through the Antioxidant Transcription Factor NFE2L2 in Human Mammary Epithelial Cells *"

    Article Title: B-cell Translocation Gene 2 (BTG2) Stimulates Cellular Antioxidant Defenses through the Antioxidant Transcription Factor NFE2L2 in Human Mammary Epithelial Cells *

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.M112.367433

    BTG2 protects against oxidative stress. A and B , MCF-7 cells were transfected overnight with wtBTG2 or empty pcDNA3 vector; harvested using trypsin; seeded into 96-well dishes; allowed to attach; exposed to different concentrations of H 2 O 2 for 24 h; and
    Figure Legend Snippet: BTG2 protects against oxidative stress. A and B , MCF-7 cells were transfected overnight with wtBTG2 or empty pcDNA3 vector; harvested using trypsin; seeded into 96-well dishes; allowed to attach; exposed to different concentrations of H 2 O 2 for 24 h; and

    Techniques Used: Transfection, Plasmid Preparation

    BTG2 regulates NFE2L2/ARE signaling. MCF-7 cells were co-transfected with NQO1-ARE-Luc reporter and the indicated expression vector(s); postincubated for 24 h; and harvested for luciferase assays. The total transfected DNA content was kept constant by
    Figure Legend Snippet: BTG2 regulates NFE2L2/ARE signaling. MCF-7 cells were co-transfected with NQO1-ARE-Luc reporter and the indicated expression vector(s); postincubated for 24 h; and harvested for luciferase assays. The total transfected DNA content was kept constant by

    Techniques Used: Transfection, Expressing, Plasmid Preparation, Luciferase

    BTG2 physically associates with NFE2L2. A and B , MCF-7 cells were transfected with FLAG-BTG2 or empty pCMV6-FLAG vector; postincubated for 24 h; and subjected to anti-FLAG ( A ) or anti-NFE2L2 ( B ) IP. Precipitated proteins were Western blotted using anti-FLAG
    Figure Legend Snippet: BTG2 physically associates with NFE2L2. A and B , MCF-7 cells were transfected with FLAG-BTG2 or empty pCMV6-FLAG vector; postincubated for 24 h; and subjected to anti-FLAG ( A ) or anti-NFE2L2 ( B ) IP. Precipitated proteins were Western blotted using anti-FLAG

    Techniques Used: Transfection, Plasmid Preparation, Western Blot

    BTG2 up-regulates expression of antioxidant enzymes. A and B , MCF-7 cells were transfected overnight with wtBTG2 or pcDNA3 vector; postincubated for 24 h to allow gene expression; harvested; and subjected to semiquantitative RT-PCR ( A ) or Western blot
    Figure Legend Snippet: BTG2 up-regulates expression of antioxidant enzymes. A and B , MCF-7 cells were transfected overnight with wtBTG2 or pcDNA3 vector; postincubated for 24 h to allow gene expression; harvested; and subjected to semiquantitative RT-PCR ( A ) or Western blot

    Techniques Used: Expressing, Transfection, Plasmid Preparation, Reverse Transcription Polymerase Chain Reaction, Western Blot

    BTG2 induces growth arrest of H 2 O 2 -treated cells. A , MCF-7 cells were transfected with wtBTG2 or pcDNA3 vector and treated with different concentrations of H 2 O 2 for 24 h, with exposure to BrdU during the last 12 h of H 2 O 2 treatment. The percentage of
    Figure Legend Snippet: BTG2 induces growth arrest of H 2 O 2 -treated cells. A , MCF-7 cells were transfected with wtBTG2 or pcDNA3 vector and treated with different concentrations of H 2 O 2 for 24 h, with exposure to BrdU during the last 12 h of H 2 O 2 treatment. The percentage of

    Techniques Used: Transfection, Plasmid Preparation

    Box B of BTG2 is required for association of BTG2 with NFE2L2 and for BTG2-mediated protection against oxidative stress in MCF-7 cells. A , schematic diagrams showing the structures of the proteins encoded by the wt-HA-BTG2, HA-BTG2-ΔA, HA-BTG2-ΔB,
    Figure Legend Snippet: Box B of BTG2 is required for association of BTG2 with NFE2L2 and for BTG2-mediated protection against oxidative stress in MCF-7 cells. A , schematic diagrams showing the structures of the proteins encoded by the wt-HA-BTG2, HA-BTG2-ΔA, HA-BTG2-ΔB,

    Techniques Used:

    BTG2 and NFE2L2 are present at the ARE. A , untransfected MCF-7 cells were subjected to ChIP assays using anti-NFE2L2 antibody or nonimmune rabbit IgG (negative control). The PCR primers correspond to two different AREs (E1 and E2) of the HO-1 gene, HO-1
    Figure Legend Snippet: BTG2 and NFE2L2 are present at the ARE. A , untransfected MCF-7 cells were subjected to ChIP assays using anti-NFE2L2 antibody or nonimmune rabbit IgG (negative control). The PCR primers correspond to two different AREs (E1 and E2) of the HO-1 gene, HO-1

    Techniques Used: Chromatin Immunoprecipitation, Negative Control, Polymerase Chain Reaction

    BTG2 regulates antioxidant enzyme activity and cellular redox state. A and B , MCF-7 cells were transfected overnight with wtBTG2 or pcDNA3 vector; postincubated for 24 h; and assayed to determine catalase ( A ) or total SOD ( B ) activity. C and D , 184A1
    Figure Legend Snippet: BTG2 regulates antioxidant enzyme activity and cellular redox state. A and B , MCF-7 cells were transfected overnight with wtBTG2 or pcDNA3 vector; postincubated for 24 h; and assayed to determine catalase ( A ) or total SOD ( B ) activity. C and D , 184A1

    Techniques Used: Activity Assay, Transfection, Plasmid Preparation

    12) Product Images from "The B Cell Translocation Gene (BTG) Family in the Rat Ovary: Hormonal Induction, Regulation, and Impact on Cell Cycle Kinetics"

    Article Title: The B Cell Translocation Gene (BTG) Family in the Rat Ovary: Hormonal Induction, Regulation, and Impact on Cell Cycle Kinetics

    Journal: Endocrinology

    doi: 10.1210/en.2008-1650

    Regulation of Btg1 and Btg2 mRNA expression by activators or inhibitors of intracellular signaling pathways in granulosa cells in vitro . A and B, Real-time PCR shows mRNA expression for Btg1 (A) and Btg2 (B) in granulosa cells from rat preovulatory ovaries
    Figure Legend Snippet: Regulation of Btg1 and Btg2 mRNA expression by activators or inhibitors of intracellular signaling pathways in granulosa cells in vitro . A and B, Real-time PCR shows mRNA expression for Btg1 (A) and Btg2 (B) in granulosa cells from rat preovulatory ovaries

    Techniques Used: Expressing, In Vitro, Real-time Polymerase Chain Reaction

    Induction of BTG1, BTG2, and BTG3 expression by hCG in rat granulosa cells in vivo . Real-time PCR analysis shows the expression of Btg1 (A), Btg2 (B), and Btg3 (C) mRNA in rat granulosa/luteal cells obtained from PMSG-primed rat preovulatory ovaries collected
    Figure Legend Snippet: Induction of BTG1, BTG2, and BTG3 expression by hCG in rat granulosa cells in vivo . Real-time PCR analysis shows the expression of Btg1 (A), Btg2 (B), and Btg3 (C) mRNA in rat granulosa/luteal cells obtained from PMSG-primed rat preovulatory ovaries collected

    Techniques Used: Expressing, In Vivo, Real-time Polymerase Chain Reaction

    Cellular localization of Btg1 , Btg2 , and Btg3 mRNA in periovulatory rat ovaries. Sections of rat ovaries obtained at 0 h (48 h after PMSG) (panel A), 4 h (panel B), 8 h (panels C, I, and J), 12 h (panel D), or 24 h (panels E–H) after hCG injection
    Figure Legend Snippet: Cellular localization of Btg1 , Btg2 , and Btg3 mRNA in periovulatory rat ovaries. Sections of rat ovaries obtained at 0 h (48 h after PMSG) (panel A), 4 h (panel B), 8 h (panels C, I, and J), 12 h (panel D), or 24 h (panels E–H) after hCG injection

    Techniques Used: Injection

    Stimulation of Btg1 (A) and Btg2 (B) mRNA expression by hCG in rat granulosa cells in vitro . Real-time PCR analysis shows the expression of Btg1 and Btg2 mRNA in granulosa cells obtained from rat preovulatory ovaries (48 h after PMSG) and cultured in
    Figure Legend Snippet: Stimulation of Btg1 (A) and Btg2 (B) mRNA expression by hCG in rat granulosa cells in vitro . Real-time PCR analysis shows the expression of Btg1 and Btg2 mRNA in granulosa cells obtained from rat preovulatory ovaries (48 h after PMSG) and cultured in

    Techniques Used: Expressing, In Vitro, Real-time Polymerase Chain Reaction, Cell Culture

    Cell cycle analysis of granulosa cells after overexpression of rat BTG1 and BTG2. A, Western blot shows that both BTG1 and BTG2 are highly expressed in granulosa cells that were infected with the Ad-BTG1 or Ad-BTG2 adenovirus vector for 48 h. B and C,
    Figure Legend Snippet: Cell cycle analysis of granulosa cells after overexpression of rat BTG1 and BTG2. A, Western blot shows that both BTG1 and BTG2 are highly expressed in granulosa cells that were infected with the Ad-BTG1 or Ad-BTG2 adenovirus vector for 48 h. B and C,

    Techniques Used: Cell Cycle Assay, Over Expression, Western Blot, Infection, Plasmid Preparation

    Stimulation of BTG1, BTG2, and BTG3 expression by hCG in rat ovary. Real-time PCR analysis shows the expression of Btg1 (A), Btg2 (B), and Btg3 (C) mRNA in preovulatory ovaries after hCG administration. Rats were injected with PMSG for 48 h and treated
    Figure Legend Snippet: Stimulation of BTG1, BTG2, and BTG3 expression by hCG in rat ovary. Real-time PCR analysis shows the expression of Btg1 (A), Btg2 (B), and Btg3 (C) mRNA in preovulatory ovaries after hCG administration. Rats were injected with PMSG for 48 h and treated

    Techniques Used: Expressing, Real-time Polymerase Chain Reaction, Injection

    13) Product Images from "The B Cell Translocation Gene (BTG) Family in the Rat Ovary: Hormonal Induction, Regulation, and Impact on Cell Cycle Kinetics"

    Article Title: The B Cell Translocation Gene (BTG) Family in the Rat Ovary: Hormonal Induction, Regulation, and Impact on Cell Cycle Kinetics

    Journal: Endocrinology

    doi: 10.1210/en.2008-1650

    Regulation of Btg1 and Btg2 mRNA expression by activators or inhibitors of intracellular signaling pathways in granulosa cells in vitro . A and B, Real-time PCR shows mRNA expression for Btg1 (A) and Btg2 (B) in granulosa cells from rat preovulatory ovaries
    Figure Legend Snippet: Regulation of Btg1 and Btg2 mRNA expression by activators or inhibitors of intracellular signaling pathways in granulosa cells in vitro . A and B, Real-time PCR shows mRNA expression for Btg1 (A) and Btg2 (B) in granulosa cells from rat preovulatory ovaries

    Techniques Used: Expressing, In Vitro, Real-time Polymerase Chain Reaction

    Induction of BTG1, BTG2, and BTG3 expression by hCG in rat granulosa cells in vivo . Real-time PCR analysis shows the expression of Btg1 (A), Btg2 (B), and Btg3 (C) mRNA in rat granulosa/luteal cells obtained from PMSG-primed rat preovulatory ovaries collected
    Figure Legend Snippet: Induction of BTG1, BTG2, and BTG3 expression by hCG in rat granulosa cells in vivo . Real-time PCR analysis shows the expression of Btg1 (A), Btg2 (B), and Btg3 (C) mRNA in rat granulosa/luteal cells obtained from PMSG-primed rat preovulatory ovaries collected

    Techniques Used: Expressing, In Vivo, Real-time Polymerase Chain Reaction

    Cellular localization of Btg1 , Btg2 , and Btg3 mRNA in periovulatory rat ovaries. Sections of rat ovaries obtained at 0 h (48 h after PMSG) (panel A), 4 h (panel B), 8 h (panels C, I, and J), 12 h (panel D), or 24 h (panels E–H) after hCG injection
    Figure Legend Snippet: Cellular localization of Btg1 , Btg2 , and Btg3 mRNA in periovulatory rat ovaries. Sections of rat ovaries obtained at 0 h (48 h after PMSG) (panel A), 4 h (panel B), 8 h (panels C, I, and J), 12 h (panel D), or 24 h (panels E–H) after hCG injection

    Techniques Used: Injection

    Stimulation of Btg1 (A) and Btg2 (B) mRNA expression by hCG in rat granulosa cells in vitro . Real-time PCR analysis shows the expression of Btg1 and Btg2 mRNA in granulosa cells obtained from rat preovulatory ovaries (48 h after PMSG) and cultured in
    Figure Legend Snippet: Stimulation of Btg1 (A) and Btg2 (B) mRNA expression by hCG in rat granulosa cells in vitro . Real-time PCR analysis shows the expression of Btg1 and Btg2 mRNA in granulosa cells obtained from rat preovulatory ovaries (48 h after PMSG) and cultured in

    Techniques Used: Expressing, In Vitro, Real-time Polymerase Chain Reaction, Cell Culture

    Cell cycle analysis of granulosa cells after overexpression of rat BTG1 and BTG2. A, Western blot shows that both BTG1 and BTG2 are highly expressed in granulosa cells that were infected with the Ad-BTG1 or Ad-BTG2 adenovirus vector for 48 h. B and C,
    Figure Legend Snippet: Cell cycle analysis of granulosa cells after overexpression of rat BTG1 and BTG2. A, Western blot shows that both BTG1 and BTG2 are highly expressed in granulosa cells that were infected with the Ad-BTG1 or Ad-BTG2 adenovirus vector for 48 h. B and C,

    Techniques Used: Cell Cycle Assay, Over Expression, Western Blot, Infection, Plasmid Preparation

    Stimulation of BTG1, BTG2, and BTG3 expression by hCG in rat ovary. Real-time PCR analysis shows the expression of Btg1 (A), Btg2 (B), and Btg3 (C) mRNA in preovulatory ovaries after hCG administration. Rats were injected with PMSG for 48 h and treated
    Figure Legend Snippet: Stimulation of BTG1, BTG2, and BTG3 expression by hCG in rat ovary. Real-time PCR analysis shows the expression of Btg1 (A), Btg2 (B), and Btg3 (C) mRNA in preovulatory ovaries after hCG administration. Rats were injected with PMSG for 48 h and treated

    Techniques Used: Expressing, Real-time Polymerase Chain Reaction, Injection

    14) Product Images from "B-cell Translocation Gene 2 (BTG2) Stimulates Cellular Antioxidant Defenses through the Antioxidant Transcription Factor NFE2L2 in Human Mammary Epithelial Cells *"

    Article Title: B-cell Translocation Gene 2 (BTG2) Stimulates Cellular Antioxidant Defenses through the Antioxidant Transcription Factor NFE2L2 in Human Mammary Epithelial Cells *

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.M112.367433

    BTG2 protects against oxidative stress. A and B , MCF-7 cells were transfected overnight with wtBTG2 or empty pcDNA3 vector; harvested using trypsin; seeded into 96-well dishes; allowed to attach; exposed to different concentrations of H 2 O 2 for 24 h; and
    Figure Legend Snippet: BTG2 protects against oxidative stress. A and B , MCF-7 cells were transfected overnight with wtBTG2 or empty pcDNA3 vector; harvested using trypsin; seeded into 96-well dishes; allowed to attach; exposed to different concentrations of H 2 O 2 for 24 h; and

    Techniques Used: Transfection, Plasmid Preparation

    BTG2 regulates NFE2L2/ARE signaling. MCF-7 cells were co-transfected with NQO1-ARE-Luc reporter and the indicated expression vector(s); postincubated for 24 h; and harvested for luciferase assays. The total transfected DNA content was kept constant by
    Figure Legend Snippet: BTG2 regulates NFE2L2/ARE signaling. MCF-7 cells were co-transfected with NQO1-ARE-Luc reporter and the indicated expression vector(s); postincubated for 24 h; and harvested for luciferase assays. The total transfected DNA content was kept constant by

    Techniques Used: Transfection, Expressing, Plasmid Preparation, Luciferase

    BTG2 physically associates with NFE2L2. A and B , MCF-7 cells were transfected with FLAG-BTG2 or empty pCMV6-FLAG vector; postincubated for 24 h; and subjected to anti-FLAG ( A ) or anti-NFE2L2 ( B ) IP. Precipitated proteins were Western blotted using anti-FLAG
    Figure Legend Snippet: BTG2 physically associates with NFE2L2. A and B , MCF-7 cells were transfected with FLAG-BTG2 or empty pCMV6-FLAG vector; postincubated for 24 h; and subjected to anti-FLAG ( A ) or anti-NFE2L2 ( B ) IP. Precipitated proteins were Western blotted using anti-FLAG

    Techniques Used: Transfection, Plasmid Preparation, Western Blot

    BTG2 up-regulates expression of antioxidant enzymes. A and B , MCF-7 cells were transfected overnight with wtBTG2 or pcDNA3 vector; postincubated for 24 h to allow gene expression; harvested; and subjected to semiquantitative RT-PCR ( A ) or Western blot
    Figure Legend Snippet: BTG2 up-regulates expression of antioxidant enzymes. A and B , MCF-7 cells were transfected overnight with wtBTG2 or pcDNA3 vector; postincubated for 24 h to allow gene expression; harvested; and subjected to semiquantitative RT-PCR ( A ) or Western blot

    Techniques Used: Expressing, Transfection, Plasmid Preparation, Reverse Transcription Polymerase Chain Reaction, Western Blot

    BTG2 induces growth arrest of H 2 O 2 -treated cells. A , MCF-7 cells were transfected with wtBTG2 or pcDNA3 vector and treated with different concentrations of H 2 O 2 for 24 h, with exposure to BrdU during the last 12 h of H 2 O 2 treatment. The percentage of
    Figure Legend Snippet: BTG2 induces growth arrest of H 2 O 2 -treated cells. A , MCF-7 cells were transfected with wtBTG2 or pcDNA3 vector and treated with different concentrations of H 2 O 2 for 24 h, with exposure to BrdU during the last 12 h of H 2 O 2 treatment. The percentage of

    Techniques Used: Transfection, Plasmid Preparation

    Box B of BTG2 is required for association of BTG2 with NFE2L2 and for BTG2-mediated protection against oxidative stress in MCF-7 cells. A , schematic diagrams showing the structures of the proteins encoded by the wt-HA-BTG2, HA-BTG2-ΔA, HA-BTG2-ΔB,
    Figure Legend Snippet: Box B of BTG2 is required for association of BTG2 with NFE2L2 and for BTG2-mediated protection against oxidative stress in MCF-7 cells. A , schematic diagrams showing the structures of the proteins encoded by the wt-HA-BTG2, HA-BTG2-ΔA, HA-BTG2-ΔB,

    Techniques Used:

    BTG2 and NFE2L2 are present at the ARE. A , untransfected MCF-7 cells were subjected to ChIP assays using anti-NFE2L2 antibody or nonimmune rabbit IgG (negative control). The PCR primers correspond to two different AREs (E1 and E2) of the HO-1 gene, HO-1
    Figure Legend Snippet: BTG2 and NFE2L2 are present at the ARE. A , untransfected MCF-7 cells were subjected to ChIP assays using anti-NFE2L2 antibody or nonimmune rabbit IgG (negative control). The PCR primers correspond to two different AREs (E1 and E2) of the HO-1 gene, HO-1

    Techniques Used: Chromatin Immunoprecipitation, Negative Control, Polymerase Chain Reaction

    BTG2 regulates antioxidant enzyme activity and cellular redox state. A and B , MCF-7 cells were transfected overnight with wtBTG2 or pcDNA3 vector; postincubated for 24 h; and assayed to determine catalase ( A ) or total SOD ( B ) activity. C and D , 184A1
    Figure Legend Snippet: BTG2 regulates antioxidant enzyme activity and cellular redox state. A and B , MCF-7 cells were transfected overnight with wtBTG2 or pcDNA3 vector; postincubated for 24 h; and assayed to determine catalase ( A ) or total SOD ( B ) activity. C and D , 184A1

    Techniques Used: Activity Assay, Transfection, Plasmid Preparation

    15) Product Images from "The B Cell Translocation Gene (BTG) Family in the Rat Ovary: Hormonal Induction, Regulation, and Impact on Cell Cycle Kinetics"

    Article Title: The B Cell Translocation Gene (BTG) Family in the Rat Ovary: Hormonal Induction, Regulation, and Impact on Cell Cycle Kinetics

    Journal: Endocrinology

    doi: 10.1210/en.2008-1650

    Regulation of Btg1 and Btg2 mRNA expression by activators or inhibitors of intracellular signaling pathways in granulosa cells in vitro . A and B, Real-time PCR shows mRNA expression for Btg1 (A) and Btg2 (B) in granulosa cells from rat preovulatory ovaries
    Figure Legend Snippet: Regulation of Btg1 and Btg2 mRNA expression by activators or inhibitors of intracellular signaling pathways in granulosa cells in vitro . A and B, Real-time PCR shows mRNA expression for Btg1 (A) and Btg2 (B) in granulosa cells from rat preovulatory ovaries

    Techniques Used: Expressing, In Vitro, Real-time Polymerase Chain Reaction

    Induction of BTG1, BTG2, and BTG3 expression by hCG in rat granulosa cells in vivo . Real-time PCR analysis shows the expression of Btg1 (A), Btg2 (B), and Btg3 (C) mRNA in rat granulosa/luteal cells obtained from PMSG-primed rat preovulatory ovaries collected
    Figure Legend Snippet: Induction of BTG1, BTG2, and BTG3 expression by hCG in rat granulosa cells in vivo . Real-time PCR analysis shows the expression of Btg1 (A), Btg2 (B), and Btg3 (C) mRNA in rat granulosa/luteal cells obtained from PMSG-primed rat preovulatory ovaries collected

    Techniques Used: Expressing, In Vivo, Real-time Polymerase Chain Reaction

    Cellular localization of Btg1 , Btg2 , and Btg3 mRNA in periovulatory rat ovaries. Sections of rat ovaries obtained at 0 h (48 h after PMSG) (panel A), 4 h (panel B), 8 h (panels C, I, and J), 12 h (panel D), or 24 h (panels E–H) after hCG injection
    Figure Legend Snippet: Cellular localization of Btg1 , Btg2 , and Btg3 mRNA in periovulatory rat ovaries. Sections of rat ovaries obtained at 0 h (48 h after PMSG) (panel A), 4 h (panel B), 8 h (panels C, I, and J), 12 h (panel D), or 24 h (panels E–H) after hCG injection

    Techniques Used: Injection

    Stimulation of Btg1 (A) and Btg2 (B) mRNA expression by hCG in rat granulosa cells in vitro . Real-time PCR analysis shows the expression of Btg1 and Btg2 mRNA in granulosa cells obtained from rat preovulatory ovaries (48 h after PMSG) and cultured in
    Figure Legend Snippet: Stimulation of Btg1 (A) and Btg2 (B) mRNA expression by hCG in rat granulosa cells in vitro . Real-time PCR analysis shows the expression of Btg1 and Btg2 mRNA in granulosa cells obtained from rat preovulatory ovaries (48 h after PMSG) and cultured in

    Techniques Used: Expressing, In Vitro, Real-time Polymerase Chain Reaction, Cell Culture

    Cell cycle analysis of granulosa cells after overexpression of rat BTG1 and BTG2. A, Western blot shows that both BTG1 and BTG2 are highly expressed in granulosa cells that were infected with the Ad-BTG1 or Ad-BTG2 adenovirus vector for 48 h. B and C,
    Figure Legend Snippet: Cell cycle analysis of granulosa cells after overexpression of rat BTG1 and BTG2. A, Western blot shows that both BTG1 and BTG2 are highly expressed in granulosa cells that were infected with the Ad-BTG1 or Ad-BTG2 adenovirus vector for 48 h. B and C,

    Techniques Used: Cell Cycle Assay, Over Expression, Western Blot, Infection, Plasmid Preparation

    Stimulation of BTG1, BTG2, and BTG3 expression by hCG in rat ovary. Real-time PCR analysis shows the expression of Btg1 (A), Btg2 (B), and Btg3 (C) mRNA in preovulatory ovaries after hCG administration. Rats were injected with PMSG for 48 h and treated
    Figure Legend Snippet: Stimulation of BTG1, BTG2, and BTG3 expression by hCG in rat ovary. Real-time PCR analysis shows the expression of Btg1 (A), Btg2 (B), and Btg3 (C) mRNA in preovulatory ovaries after hCG administration. Rats were injected with PMSG for 48 h and treated

    Techniques Used: Expressing, Real-time Polymerase Chain Reaction, Injection

    16) Product Images from "B-cell Translocation Gene 2 (BTG2) Stimulates Cellular Antioxidant Defenses through the Antioxidant Transcription Factor NFE2L2 in Human Mammary Epithelial Cells *"

    Article Title: B-cell Translocation Gene 2 (BTG2) Stimulates Cellular Antioxidant Defenses through the Antioxidant Transcription Factor NFE2L2 in Human Mammary Epithelial Cells *

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.M112.367433

    BTG2 protects against oxidative stress. A and B , MCF-7 cells were transfected overnight with wtBTG2 or empty pcDNA3 vector; harvested using trypsin; seeded into 96-well dishes; allowed to attach; exposed to different concentrations of H 2 O 2 for 24 h; and
    Figure Legend Snippet: BTG2 protects against oxidative stress. A and B , MCF-7 cells were transfected overnight with wtBTG2 or empty pcDNA3 vector; harvested using trypsin; seeded into 96-well dishes; allowed to attach; exposed to different concentrations of H 2 O 2 for 24 h; and

    Techniques Used: Transfection, Plasmid Preparation

    BTG2 regulates NFE2L2/ARE signaling. MCF-7 cells were co-transfected with NQO1-ARE-Luc reporter and the indicated expression vector(s); postincubated for 24 h; and harvested for luciferase assays. The total transfected DNA content was kept constant by
    Figure Legend Snippet: BTG2 regulates NFE2L2/ARE signaling. MCF-7 cells were co-transfected with NQO1-ARE-Luc reporter and the indicated expression vector(s); postincubated for 24 h; and harvested for luciferase assays. The total transfected DNA content was kept constant by

    Techniques Used: Transfection, Expressing, Plasmid Preparation, Luciferase

    BTG2 physically associates with NFE2L2. A and B , MCF-7 cells were transfected with FLAG-BTG2 or empty pCMV6-FLAG vector; postincubated for 24 h; and subjected to anti-FLAG ( A ) or anti-NFE2L2 ( B ) IP. Precipitated proteins were Western blotted using anti-FLAG
    Figure Legend Snippet: BTG2 physically associates with NFE2L2. A and B , MCF-7 cells were transfected with FLAG-BTG2 or empty pCMV6-FLAG vector; postincubated for 24 h; and subjected to anti-FLAG ( A ) or anti-NFE2L2 ( B ) IP. Precipitated proteins were Western blotted using anti-FLAG

    Techniques Used: Transfection, Plasmid Preparation, Western Blot

    BTG2 up-regulates expression of antioxidant enzymes. A and B , MCF-7 cells were transfected overnight with wtBTG2 or pcDNA3 vector; postincubated for 24 h to allow gene expression; harvested; and subjected to semiquantitative RT-PCR ( A ) or Western blot
    Figure Legend Snippet: BTG2 up-regulates expression of antioxidant enzymes. A and B , MCF-7 cells were transfected overnight with wtBTG2 or pcDNA3 vector; postincubated for 24 h to allow gene expression; harvested; and subjected to semiquantitative RT-PCR ( A ) or Western blot

    Techniques Used: Expressing, Transfection, Plasmid Preparation, Reverse Transcription Polymerase Chain Reaction, Western Blot

    BTG2 induces growth arrest of H 2 O 2 -treated cells. A , MCF-7 cells were transfected with wtBTG2 or pcDNA3 vector and treated with different concentrations of H 2 O 2 for 24 h, with exposure to BrdU during the last 12 h of H 2 O 2 treatment. The percentage of
    Figure Legend Snippet: BTG2 induces growth arrest of H 2 O 2 -treated cells. A , MCF-7 cells were transfected with wtBTG2 or pcDNA3 vector and treated with different concentrations of H 2 O 2 for 24 h, with exposure to BrdU during the last 12 h of H 2 O 2 treatment. The percentage of

    Techniques Used: Transfection, Plasmid Preparation

    Box B of BTG2 is required for association of BTG2 with NFE2L2 and for BTG2-mediated protection against oxidative stress in MCF-7 cells. A , schematic diagrams showing the structures of the proteins encoded by the wt-HA-BTG2, HA-BTG2-ΔA, HA-BTG2-ΔB,
    Figure Legend Snippet: Box B of BTG2 is required for association of BTG2 with NFE2L2 and for BTG2-mediated protection against oxidative stress in MCF-7 cells. A , schematic diagrams showing the structures of the proteins encoded by the wt-HA-BTG2, HA-BTG2-ΔA, HA-BTG2-ΔB,

    Techniques Used:

    BTG2 and NFE2L2 are present at the ARE. A , untransfected MCF-7 cells were subjected to ChIP assays using anti-NFE2L2 antibody or nonimmune rabbit IgG (negative control). The PCR primers correspond to two different AREs (E1 and E2) of the HO-1 gene, HO-1
    Figure Legend Snippet: BTG2 and NFE2L2 are present at the ARE. A , untransfected MCF-7 cells were subjected to ChIP assays using anti-NFE2L2 antibody or nonimmune rabbit IgG (negative control). The PCR primers correspond to two different AREs (E1 and E2) of the HO-1 gene, HO-1

    Techniques Used: Chromatin Immunoprecipitation, Negative Control, Polymerase Chain Reaction

    BTG2 regulates antioxidant enzyme activity and cellular redox state. A and B , MCF-7 cells were transfected overnight with wtBTG2 or pcDNA3 vector; postincubated for 24 h; and assayed to determine catalase ( A ) or total SOD ( B ) activity. C and D , 184A1
    Figure Legend Snippet: BTG2 regulates antioxidant enzyme activity and cellular redox state. A and B , MCF-7 cells were transfected overnight with wtBTG2 or pcDNA3 vector; postincubated for 24 h; and assayed to determine catalase ( A ) or total SOD ( B ) activity. C and D , 184A1

    Techniques Used: Activity Assay, Transfection, Plasmid Preparation

    17) Product Images from "The B Cell Translocation Gene (BTG) Family in the Rat Ovary: Hormonal Induction, Regulation, and Impact on Cell Cycle Kinetics"

    Article Title: The B Cell Translocation Gene (BTG) Family in the Rat Ovary: Hormonal Induction, Regulation, and Impact on Cell Cycle Kinetics

    Journal: Endocrinology

    doi: 10.1210/en.2008-1650

    Regulation of Btg1 and Btg2 mRNA expression by activators or inhibitors of intracellular signaling pathways in granulosa cells in vitro . A and B, Real-time PCR shows mRNA expression for Btg1 (A) and Btg2 (B) in granulosa cells from rat preovulatory ovaries
    Figure Legend Snippet: Regulation of Btg1 and Btg2 mRNA expression by activators or inhibitors of intracellular signaling pathways in granulosa cells in vitro . A and B, Real-time PCR shows mRNA expression for Btg1 (A) and Btg2 (B) in granulosa cells from rat preovulatory ovaries

    Techniques Used: Expressing, In Vitro, Real-time Polymerase Chain Reaction

    Induction of BTG1, BTG2, and BTG3 expression by hCG in rat granulosa cells in vivo . Real-time PCR analysis shows the expression of Btg1 (A), Btg2 (B), and Btg3 (C) mRNA in rat granulosa/luteal cells obtained from PMSG-primed rat preovulatory ovaries collected
    Figure Legend Snippet: Induction of BTG1, BTG2, and BTG3 expression by hCG in rat granulosa cells in vivo . Real-time PCR analysis shows the expression of Btg1 (A), Btg2 (B), and Btg3 (C) mRNA in rat granulosa/luteal cells obtained from PMSG-primed rat preovulatory ovaries collected

    Techniques Used: Expressing, In Vivo, Real-time Polymerase Chain Reaction

    Cellular localization of Btg1 , Btg2 , and Btg3 mRNA in periovulatory rat ovaries. Sections of rat ovaries obtained at 0 h (48 h after PMSG) (panel A), 4 h (panel B), 8 h (panels C, I, and J), 12 h (panel D), or 24 h (panels E–H) after hCG injection
    Figure Legend Snippet: Cellular localization of Btg1 , Btg2 , and Btg3 mRNA in periovulatory rat ovaries. Sections of rat ovaries obtained at 0 h (48 h after PMSG) (panel A), 4 h (panel B), 8 h (panels C, I, and J), 12 h (panel D), or 24 h (panels E–H) after hCG injection

    Techniques Used: Injection

    Stimulation of Btg1 (A) and Btg2 (B) mRNA expression by hCG in rat granulosa cells in vitro . Real-time PCR analysis shows the expression of Btg1 and Btg2 mRNA in granulosa cells obtained from rat preovulatory ovaries (48 h after PMSG) and cultured in
    Figure Legend Snippet: Stimulation of Btg1 (A) and Btg2 (B) mRNA expression by hCG in rat granulosa cells in vitro . Real-time PCR analysis shows the expression of Btg1 and Btg2 mRNA in granulosa cells obtained from rat preovulatory ovaries (48 h after PMSG) and cultured in

    Techniques Used: Expressing, In Vitro, Real-time Polymerase Chain Reaction, Cell Culture

    Cell cycle analysis of granulosa cells after overexpression of rat BTG1 and BTG2. A, Western blot shows that both BTG1 and BTG2 are highly expressed in granulosa cells that were infected with the Ad-BTG1 or Ad-BTG2 adenovirus vector for 48 h. B and C,
    Figure Legend Snippet: Cell cycle analysis of granulosa cells after overexpression of rat BTG1 and BTG2. A, Western blot shows that both BTG1 and BTG2 are highly expressed in granulosa cells that were infected with the Ad-BTG1 or Ad-BTG2 adenovirus vector for 48 h. B and C,

    Techniques Used: Cell Cycle Assay, Over Expression, Western Blot, Infection, Plasmid Preparation

    Stimulation of BTG1, BTG2, and BTG3 expression by hCG in rat ovary. Real-time PCR analysis shows the expression of Btg1 (A), Btg2 (B), and Btg3 (C) mRNA in preovulatory ovaries after hCG administration. Rats were injected with PMSG for 48 h and treated
    Figure Legend Snippet: Stimulation of BTG1, BTG2, and BTG3 expression by hCG in rat ovary. Real-time PCR analysis shows the expression of Btg1 (A), Btg2 (B), and Btg3 (C) mRNA in preovulatory ovaries after hCG administration. Rats were injected with PMSG for 48 h and treated

    Techniques Used: Expressing, Real-time Polymerase Chain Reaction, Injection

    18) Product Images from "The B Cell Translocation Gene (BTG) Family in the Rat Ovary: Hormonal Induction, Regulation, and Impact on Cell Cycle Kinetics"

    Article Title: The B Cell Translocation Gene (BTG) Family in the Rat Ovary: Hormonal Induction, Regulation, and Impact on Cell Cycle Kinetics

    Journal: Endocrinology

    doi: 10.1210/en.2008-1650

    Regulation of Btg1 and Btg2 mRNA expression by activators or inhibitors of intracellular signaling pathways in granulosa cells in vitro . A and B, Real-time PCR shows mRNA expression for Btg1 (A) and Btg2 (B) in granulosa cells from rat preovulatory ovaries
    Figure Legend Snippet: Regulation of Btg1 and Btg2 mRNA expression by activators or inhibitors of intracellular signaling pathways in granulosa cells in vitro . A and B, Real-time PCR shows mRNA expression for Btg1 (A) and Btg2 (B) in granulosa cells from rat preovulatory ovaries

    Techniques Used: Expressing, In Vitro, Real-time Polymerase Chain Reaction

    Induction of BTG1, BTG2, and BTG3 expression by hCG in rat granulosa cells in vivo . Real-time PCR analysis shows the expression of Btg1 (A), Btg2 (B), and Btg3 (C) mRNA in rat granulosa/luteal cells obtained from PMSG-primed rat preovulatory ovaries collected
    Figure Legend Snippet: Induction of BTG1, BTG2, and BTG3 expression by hCG in rat granulosa cells in vivo . Real-time PCR analysis shows the expression of Btg1 (A), Btg2 (B), and Btg3 (C) mRNA in rat granulosa/luteal cells obtained from PMSG-primed rat preovulatory ovaries collected

    Techniques Used: Expressing, In Vivo, Real-time Polymerase Chain Reaction

    Cellular localization of Btg1 , Btg2 , and Btg3 mRNA in periovulatory rat ovaries. Sections of rat ovaries obtained at 0 h (48 h after PMSG) (panel A), 4 h (panel B), 8 h (panels C, I, and J), 12 h (panel D), or 24 h (panels E–H) after hCG injection
    Figure Legend Snippet: Cellular localization of Btg1 , Btg2 , and Btg3 mRNA in periovulatory rat ovaries. Sections of rat ovaries obtained at 0 h (48 h after PMSG) (panel A), 4 h (panel B), 8 h (panels C, I, and J), 12 h (panel D), or 24 h (panels E–H) after hCG injection

    Techniques Used: Injection

    Stimulation of Btg1 (A) and Btg2 (B) mRNA expression by hCG in rat granulosa cells in vitro . Real-time PCR analysis shows the expression of Btg1 and Btg2 mRNA in granulosa cells obtained from rat preovulatory ovaries (48 h after PMSG) and cultured in
    Figure Legend Snippet: Stimulation of Btg1 (A) and Btg2 (B) mRNA expression by hCG in rat granulosa cells in vitro . Real-time PCR analysis shows the expression of Btg1 and Btg2 mRNA in granulosa cells obtained from rat preovulatory ovaries (48 h after PMSG) and cultured in

    Techniques Used: Expressing, In Vitro, Real-time Polymerase Chain Reaction, Cell Culture

    Cell cycle analysis of granulosa cells after overexpression of rat BTG1 and BTG2. A, Western blot shows that both BTG1 and BTG2 are highly expressed in granulosa cells that were infected with the Ad-BTG1 or Ad-BTG2 adenovirus vector for 48 h. B and C,
    Figure Legend Snippet: Cell cycle analysis of granulosa cells after overexpression of rat BTG1 and BTG2. A, Western blot shows that both BTG1 and BTG2 are highly expressed in granulosa cells that were infected with the Ad-BTG1 or Ad-BTG2 adenovirus vector for 48 h. B and C,

    Techniques Used: Cell Cycle Assay, Over Expression, Western Blot, Infection, Plasmid Preparation

    Stimulation of BTG1, BTG2, and BTG3 expression by hCG in rat ovary. Real-time PCR analysis shows the expression of Btg1 (A), Btg2 (B), and Btg3 (C) mRNA in preovulatory ovaries after hCG administration. Rats were injected with PMSG for 48 h and treated
    Figure Legend Snippet: Stimulation of BTG1, BTG2, and BTG3 expression by hCG in rat ovary. Real-time PCR analysis shows the expression of Btg1 (A), Btg2 (B), and Btg3 (C) mRNA in preovulatory ovaries after hCG administration. Rats were injected with PMSG for 48 h and treated

    Techniques Used: Expressing, Real-time Polymerase Chain Reaction, Injection

    19) Product Images from "B-cell Translocation Gene 2 (BTG2) Stimulates Cellular Antioxidant Defenses through the Antioxidant Transcription Factor NFE2L2 in Human Mammary Epithelial Cells *"

    Article Title: B-cell Translocation Gene 2 (BTG2) Stimulates Cellular Antioxidant Defenses through the Antioxidant Transcription Factor NFE2L2 in Human Mammary Epithelial Cells *

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.M112.367433

    BTG2 protects against oxidative stress. A and B , MCF-7 cells were transfected overnight with wtBTG2 or empty pcDNA3 vector; harvested using trypsin; seeded into 96-well dishes; allowed to attach; exposed to different concentrations of H 2 O 2 for 24 h; and
    Figure Legend Snippet: BTG2 protects against oxidative stress. A and B , MCF-7 cells were transfected overnight with wtBTG2 or empty pcDNA3 vector; harvested using trypsin; seeded into 96-well dishes; allowed to attach; exposed to different concentrations of H 2 O 2 for 24 h; and

    Techniques Used: Transfection, Plasmid Preparation

    BTG2 regulates NFE2L2/ARE signaling. MCF-7 cells were co-transfected with NQO1-ARE-Luc reporter and the indicated expression vector(s); postincubated for 24 h; and harvested for luciferase assays. The total transfected DNA content was kept constant by
    Figure Legend Snippet: BTG2 regulates NFE2L2/ARE signaling. MCF-7 cells were co-transfected with NQO1-ARE-Luc reporter and the indicated expression vector(s); postincubated for 24 h; and harvested for luciferase assays. The total transfected DNA content was kept constant by

    Techniques Used: Transfection, Expressing, Plasmid Preparation, Luciferase

    BTG2 physically associates with NFE2L2. A and B , MCF-7 cells were transfected with FLAG-BTG2 or empty pCMV6-FLAG vector; postincubated for 24 h; and subjected to anti-FLAG ( A ) or anti-NFE2L2 ( B ) IP. Precipitated proteins were Western blotted using anti-FLAG
    Figure Legend Snippet: BTG2 physically associates with NFE2L2. A and B , MCF-7 cells were transfected with FLAG-BTG2 or empty pCMV6-FLAG vector; postincubated for 24 h; and subjected to anti-FLAG ( A ) or anti-NFE2L2 ( B ) IP. Precipitated proteins were Western blotted using anti-FLAG

    Techniques Used: Transfection, Plasmid Preparation, Western Blot

    BTG2 up-regulates expression of antioxidant enzymes. A and B , MCF-7 cells were transfected overnight with wtBTG2 or pcDNA3 vector; postincubated for 24 h to allow gene expression; harvested; and subjected to semiquantitative RT-PCR ( A ) or Western blot
    Figure Legend Snippet: BTG2 up-regulates expression of antioxidant enzymes. A and B , MCF-7 cells were transfected overnight with wtBTG2 or pcDNA3 vector; postincubated for 24 h to allow gene expression; harvested; and subjected to semiquantitative RT-PCR ( A ) or Western blot

    Techniques Used: Expressing, Transfection, Plasmid Preparation, Reverse Transcription Polymerase Chain Reaction, Western Blot

    BTG2 induces growth arrest of H 2 O 2 -treated cells. A , MCF-7 cells were transfected with wtBTG2 or pcDNA3 vector and treated with different concentrations of H 2 O 2 for 24 h, with exposure to BrdU during the last 12 h of H 2 O 2 treatment. The percentage of
    Figure Legend Snippet: BTG2 induces growth arrest of H 2 O 2 -treated cells. A , MCF-7 cells were transfected with wtBTG2 or pcDNA3 vector and treated with different concentrations of H 2 O 2 for 24 h, with exposure to BrdU during the last 12 h of H 2 O 2 treatment. The percentage of

    Techniques Used: Transfection, Plasmid Preparation

    Box B of BTG2 is required for association of BTG2 with NFE2L2 and for BTG2-mediated protection against oxidative stress in MCF-7 cells. A , schematic diagrams showing the structures of the proteins encoded by the wt-HA-BTG2, HA-BTG2-ΔA, HA-BTG2-ΔB,
    Figure Legend Snippet: Box B of BTG2 is required for association of BTG2 with NFE2L2 and for BTG2-mediated protection against oxidative stress in MCF-7 cells. A , schematic diagrams showing the structures of the proteins encoded by the wt-HA-BTG2, HA-BTG2-ΔA, HA-BTG2-ΔB,

    Techniques Used:

    BTG2 and NFE2L2 are present at the ARE. A , untransfected MCF-7 cells were subjected to ChIP assays using anti-NFE2L2 antibody or nonimmune rabbit IgG (negative control). The PCR primers correspond to two different AREs (E1 and E2) of the HO-1 gene, HO-1
    Figure Legend Snippet: BTG2 and NFE2L2 are present at the ARE. A , untransfected MCF-7 cells were subjected to ChIP assays using anti-NFE2L2 antibody or nonimmune rabbit IgG (negative control). The PCR primers correspond to two different AREs (E1 and E2) of the HO-1 gene, HO-1

    Techniques Used: Chromatin Immunoprecipitation, Negative Control, Polymerase Chain Reaction

    BTG2 regulates antioxidant enzyme activity and cellular redox state. A and B , MCF-7 cells were transfected overnight with wtBTG2 or pcDNA3 vector; postincubated for 24 h; and assayed to determine catalase ( A ) or total SOD ( B ) activity. C and D , 184A1
    Figure Legend Snippet: BTG2 regulates antioxidant enzyme activity and cellular redox state. A and B , MCF-7 cells were transfected overnight with wtBTG2 or pcDNA3 vector; postincubated for 24 h; and assayed to determine catalase ( A ) or total SOD ( B ) activity. C and D , 184A1

    Techniques Used: Activity Assay, Transfection, Plasmid Preparation

    20) Product Images from "The B Cell Translocation Gene (BTG) Family in the Rat Ovary: Hormonal Induction, Regulation, and Impact on Cell Cycle Kinetics"

    Article Title: The B Cell Translocation Gene (BTG) Family in the Rat Ovary: Hormonal Induction, Regulation, and Impact on Cell Cycle Kinetics

    Journal: Endocrinology

    doi: 10.1210/en.2008-1650

    Regulation of Btg1 and Btg2 mRNA expression by activators or inhibitors of intracellular signaling pathways in granulosa cells in vitro . A and B, Real-time PCR shows mRNA expression for Btg1 (A) and Btg2 (B) in granulosa cells from rat preovulatory ovaries
    Figure Legend Snippet: Regulation of Btg1 and Btg2 mRNA expression by activators or inhibitors of intracellular signaling pathways in granulosa cells in vitro . A and B, Real-time PCR shows mRNA expression for Btg1 (A) and Btg2 (B) in granulosa cells from rat preovulatory ovaries

    Techniques Used: Expressing, In Vitro, Real-time Polymerase Chain Reaction

    Induction of BTG1, BTG2, and BTG3 expression by hCG in rat granulosa cells in vivo . Real-time PCR analysis shows the expression of Btg1 (A), Btg2 (B), and Btg3 (C) mRNA in rat granulosa/luteal cells obtained from PMSG-primed rat preovulatory ovaries collected
    Figure Legend Snippet: Induction of BTG1, BTG2, and BTG3 expression by hCG in rat granulosa cells in vivo . Real-time PCR analysis shows the expression of Btg1 (A), Btg2 (B), and Btg3 (C) mRNA in rat granulosa/luteal cells obtained from PMSG-primed rat preovulatory ovaries collected

    Techniques Used: Expressing, In Vivo, Real-time Polymerase Chain Reaction

    Cellular localization of Btg1 , Btg2 , and Btg3 mRNA in periovulatory rat ovaries. Sections of rat ovaries obtained at 0 h (48 h after PMSG) (panel A), 4 h (panel B), 8 h (panels C, I, and J), 12 h (panel D), or 24 h (panels E–H) after hCG injection
    Figure Legend Snippet: Cellular localization of Btg1 , Btg2 , and Btg3 mRNA in periovulatory rat ovaries. Sections of rat ovaries obtained at 0 h (48 h after PMSG) (panel A), 4 h (panel B), 8 h (panels C, I, and J), 12 h (panel D), or 24 h (panels E–H) after hCG injection

    Techniques Used: Injection

    Stimulation of Btg1 (A) and Btg2 (B) mRNA expression by hCG in rat granulosa cells in vitro . Real-time PCR analysis shows the expression of Btg1 and Btg2 mRNA in granulosa cells obtained from rat preovulatory ovaries (48 h after PMSG) and cultured in
    Figure Legend Snippet: Stimulation of Btg1 (A) and Btg2 (B) mRNA expression by hCG in rat granulosa cells in vitro . Real-time PCR analysis shows the expression of Btg1 and Btg2 mRNA in granulosa cells obtained from rat preovulatory ovaries (48 h after PMSG) and cultured in

    Techniques Used: Expressing, In Vitro, Real-time Polymerase Chain Reaction, Cell Culture

    Cell cycle analysis of granulosa cells after overexpression of rat BTG1 and BTG2. A, Western blot shows that both BTG1 and BTG2 are highly expressed in granulosa cells that were infected with the Ad-BTG1 or Ad-BTG2 adenovirus vector for 48 h. B and C,
    Figure Legend Snippet: Cell cycle analysis of granulosa cells after overexpression of rat BTG1 and BTG2. A, Western blot shows that both BTG1 and BTG2 are highly expressed in granulosa cells that were infected with the Ad-BTG1 or Ad-BTG2 adenovirus vector for 48 h. B and C,

    Techniques Used: Cell Cycle Assay, Over Expression, Western Blot, Infection, Plasmid Preparation

    Stimulation of BTG1, BTG2, and BTG3 expression by hCG in rat ovary. Real-time PCR analysis shows the expression of Btg1 (A), Btg2 (B), and Btg3 (C) mRNA in preovulatory ovaries after hCG administration. Rats were injected with PMSG for 48 h and treated
    Figure Legend Snippet: Stimulation of BTG1, BTG2, and BTG3 expression by hCG in rat ovary. Real-time PCR analysis shows the expression of Btg1 (A), Btg2 (B), and Btg3 (C) mRNA in preovulatory ovaries after hCG administration. Rats were injected with PMSG for 48 h and treated

    Techniques Used: Expressing, Real-time Polymerase Chain Reaction, Injection

    21) Product Images from "B-cell Translocation Gene 2 (BTG2) Stimulates Cellular Antioxidant Defenses through the Antioxidant Transcription Factor NFE2L2 in Human Mammary Epithelial Cells *"

    Article Title: B-cell Translocation Gene 2 (BTG2) Stimulates Cellular Antioxidant Defenses through the Antioxidant Transcription Factor NFE2L2 in Human Mammary Epithelial Cells *

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.M112.367433

    BTG2 protects against oxidative stress. A and B , MCF-7 cells were transfected overnight with wtBTG2 or empty pcDNA3 vector; harvested using trypsin; seeded into 96-well dishes; allowed to attach; exposed to different concentrations of H 2 O 2 for 24 h; and
    Figure Legend Snippet: BTG2 protects against oxidative stress. A and B , MCF-7 cells were transfected overnight with wtBTG2 or empty pcDNA3 vector; harvested using trypsin; seeded into 96-well dishes; allowed to attach; exposed to different concentrations of H 2 O 2 for 24 h; and

    Techniques Used: Transfection, Plasmid Preparation

    BTG2 regulates NFE2L2/ARE signaling. MCF-7 cells were co-transfected with NQO1-ARE-Luc reporter and the indicated expression vector(s); postincubated for 24 h; and harvested for luciferase assays. The total transfected DNA content was kept constant by
    Figure Legend Snippet: BTG2 regulates NFE2L2/ARE signaling. MCF-7 cells were co-transfected with NQO1-ARE-Luc reporter and the indicated expression vector(s); postincubated for 24 h; and harvested for luciferase assays. The total transfected DNA content was kept constant by

    Techniques Used: Transfection, Expressing, Plasmid Preparation, Luciferase

    BTG2 physically associates with NFE2L2. A and B , MCF-7 cells were transfected with FLAG-BTG2 or empty pCMV6-FLAG vector; postincubated for 24 h; and subjected to anti-FLAG ( A ) or anti-NFE2L2 ( B ) IP. Precipitated proteins were Western blotted using anti-FLAG
    Figure Legend Snippet: BTG2 physically associates with NFE2L2. A and B , MCF-7 cells were transfected with FLAG-BTG2 or empty pCMV6-FLAG vector; postincubated for 24 h; and subjected to anti-FLAG ( A ) or anti-NFE2L2 ( B ) IP. Precipitated proteins were Western blotted using anti-FLAG

    Techniques Used: Transfection, Plasmid Preparation, Western Blot

    BTG2 up-regulates expression of antioxidant enzymes. A and B , MCF-7 cells were transfected overnight with wtBTG2 or pcDNA3 vector; postincubated for 24 h to allow gene expression; harvested; and subjected to semiquantitative RT-PCR ( A ) or Western blot
    Figure Legend Snippet: BTG2 up-regulates expression of antioxidant enzymes. A and B , MCF-7 cells were transfected overnight with wtBTG2 or pcDNA3 vector; postincubated for 24 h to allow gene expression; harvested; and subjected to semiquantitative RT-PCR ( A ) or Western blot

    Techniques Used: Expressing, Transfection, Plasmid Preparation, Reverse Transcription Polymerase Chain Reaction, Western Blot

    BTG2 induces growth arrest of H 2 O 2 -treated cells. A , MCF-7 cells were transfected with wtBTG2 or pcDNA3 vector and treated with different concentrations of H 2 O 2 for 24 h, with exposure to BrdU during the last 12 h of H 2 O 2 treatment. The percentage of
    Figure Legend Snippet: BTG2 induces growth arrest of H 2 O 2 -treated cells. A , MCF-7 cells were transfected with wtBTG2 or pcDNA3 vector and treated with different concentrations of H 2 O 2 for 24 h, with exposure to BrdU during the last 12 h of H 2 O 2 treatment. The percentage of

    Techniques Used: Transfection, Plasmid Preparation

    Box B of BTG2 is required for association of BTG2 with NFE2L2 and for BTG2-mediated protection against oxidative stress in MCF-7 cells. A , schematic diagrams showing the structures of the proteins encoded by the wt-HA-BTG2, HA-BTG2-ΔA, HA-BTG2-ΔB,
    Figure Legend Snippet: Box B of BTG2 is required for association of BTG2 with NFE2L2 and for BTG2-mediated protection against oxidative stress in MCF-7 cells. A , schematic diagrams showing the structures of the proteins encoded by the wt-HA-BTG2, HA-BTG2-ΔA, HA-BTG2-ΔB,

    Techniques Used:

    BTG2 and NFE2L2 are present at the ARE. A , untransfected MCF-7 cells were subjected to ChIP assays using anti-NFE2L2 antibody or nonimmune rabbit IgG (negative control). The PCR primers correspond to two different AREs (E1 and E2) of the HO-1 gene, HO-1
    Figure Legend Snippet: BTG2 and NFE2L2 are present at the ARE. A , untransfected MCF-7 cells were subjected to ChIP assays using anti-NFE2L2 antibody or nonimmune rabbit IgG (negative control). The PCR primers correspond to two different AREs (E1 and E2) of the HO-1 gene, HO-1

    Techniques Used: Chromatin Immunoprecipitation, Negative Control, Polymerase Chain Reaction

    BTG2 regulates antioxidant enzyme activity and cellular redox state. A and B , MCF-7 cells were transfected overnight with wtBTG2 or pcDNA3 vector; postincubated for 24 h; and assayed to determine catalase ( A ) or total SOD ( B ) activity. C and D , 184A1
    Figure Legend Snippet: BTG2 regulates antioxidant enzyme activity and cellular redox state. A and B , MCF-7 cells were transfected overnight with wtBTG2 or pcDNA3 vector; postincubated for 24 h; and assayed to determine catalase ( A ) or total SOD ( B ) activity. C and D , 184A1

    Techniques Used: Activity Assay, Transfection, Plasmid Preparation

    22) Product Images from "The B Cell Translocation Gene (BTG) Family in the Rat Ovary: Hormonal Induction, Regulation, and Impact on Cell Cycle Kinetics"

    Article Title: The B Cell Translocation Gene (BTG) Family in the Rat Ovary: Hormonal Induction, Regulation, and Impact on Cell Cycle Kinetics

    Journal: Endocrinology

    doi: 10.1210/en.2008-1650

    Regulation of Btg1 and Btg2 mRNA expression by activators or inhibitors of intracellular signaling pathways in granulosa cells in vitro . A and B, Real-time PCR shows mRNA expression for Btg1 (A) and Btg2 (B) in granulosa cells from rat preovulatory ovaries
    Figure Legend Snippet: Regulation of Btg1 and Btg2 mRNA expression by activators or inhibitors of intracellular signaling pathways in granulosa cells in vitro . A and B, Real-time PCR shows mRNA expression for Btg1 (A) and Btg2 (B) in granulosa cells from rat preovulatory ovaries

    Techniques Used: Expressing, In Vitro, Real-time Polymerase Chain Reaction

    Induction of BTG1, BTG2, and BTG3 expression by hCG in rat granulosa cells in vivo . Real-time PCR analysis shows the expression of Btg1 (A), Btg2 (B), and Btg3 (C) mRNA in rat granulosa/luteal cells obtained from PMSG-primed rat preovulatory ovaries collected
    Figure Legend Snippet: Induction of BTG1, BTG2, and BTG3 expression by hCG in rat granulosa cells in vivo . Real-time PCR analysis shows the expression of Btg1 (A), Btg2 (B), and Btg3 (C) mRNA in rat granulosa/luteal cells obtained from PMSG-primed rat preovulatory ovaries collected

    Techniques Used: Expressing, In Vivo, Real-time Polymerase Chain Reaction

    Cellular localization of Btg1 , Btg2 , and Btg3 mRNA in periovulatory rat ovaries. Sections of rat ovaries obtained at 0 h (48 h after PMSG) (panel A), 4 h (panel B), 8 h (panels C, I, and J), 12 h (panel D), or 24 h (panels E–H) after hCG injection
    Figure Legend Snippet: Cellular localization of Btg1 , Btg2 , and Btg3 mRNA in periovulatory rat ovaries. Sections of rat ovaries obtained at 0 h (48 h after PMSG) (panel A), 4 h (panel B), 8 h (panels C, I, and J), 12 h (panel D), or 24 h (panels E–H) after hCG injection

    Techniques Used: Injection

    Stimulation of Btg1 (A) and Btg2 (B) mRNA expression by hCG in rat granulosa cells in vitro . Real-time PCR analysis shows the expression of Btg1 and Btg2 mRNA in granulosa cells obtained from rat preovulatory ovaries (48 h after PMSG) and cultured in
    Figure Legend Snippet: Stimulation of Btg1 (A) and Btg2 (B) mRNA expression by hCG in rat granulosa cells in vitro . Real-time PCR analysis shows the expression of Btg1 and Btg2 mRNA in granulosa cells obtained from rat preovulatory ovaries (48 h after PMSG) and cultured in

    Techniques Used: Expressing, In Vitro, Real-time Polymerase Chain Reaction, Cell Culture

    Cell cycle analysis of granulosa cells after overexpression of rat BTG1 and BTG2. A, Western blot shows that both BTG1 and BTG2 are highly expressed in granulosa cells that were infected with the Ad-BTG1 or Ad-BTG2 adenovirus vector for 48 h. B and C,
    Figure Legend Snippet: Cell cycle analysis of granulosa cells after overexpression of rat BTG1 and BTG2. A, Western blot shows that both BTG1 and BTG2 are highly expressed in granulosa cells that were infected with the Ad-BTG1 or Ad-BTG2 adenovirus vector for 48 h. B and C,

    Techniques Used: Cell Cycle Assay, Over Expression, Western Blot, Infection, Plasmid Preparation

    Stimulation of BTG1, BTG2, and BTG3 expression by hCG in rat ovary. Real-time PCR analysis shows the expression of Btg1 (A), Btg2 (B), and Btg3 (C) mRNA in preovulatory ovaries after hCG administration. Rats were injected with PMSG for 48 h and treated
    Figure Legend Snippet: Stimulation of BTG1, BTG2, and BTG3 expression by hCG in rat ovary. Real-time PCR analysis shows the expression of Btg1 (A), Btg2 (B), and Btg3 (C) mRNA in preovulatory ovaries after hCG administration. Rats were injected with PMSG for 48 h and treated

    Techniques Used: Expressing, Real-time Polymerase Chain Reaction, Injection

    Related Articles

    other:

    Article Title: B-cell Translocation Gene 2 (BTG2) Stimulates Cellular Antioxidant Defenses through the Antioxidant Transcription Factor NFE2L2 in Human Mammary Epithelial Cells *
    Article Snippet: Prévôt D., Morel A. P., Voeltzel T., Rostan M. C., Rimokh R., Magaud J. P., Corbo L. (2001) Relationships of the antiproliferative proteins BTG1 and BTG2 with CAF1, the human homolog of a component of the yeast CCR4 transcriptional complex: involvement in estrogen receptor α signaling pathway .

    Article Title: The B Cell Translocation Gene (BTG) Family in the Rat Ovary: Hormonal Induction, Regulation, and Impact on Cell Cycle Kinetics
    Article Snippet: J Biol Chem 275:147–153 [ ] Prévôt D, Morel AP, Voeltzel T, Rostan MC, Rimokh R, Magaud JP, Corbo L 2001 Relationships of the antiproliferative proteins BTG1 and BTG2 with CAF1, the human homolog of a component of the yeast CCR4 transcriptional complex: involvement in estrogen receptor α signaling pathway.

    Article Title: B-cell Translocation Gene 2 (BTG2) Stimulates Cellular Antioxidant Defenses through the Antioxidant Transcription Factor NFE2L2 in Human Mammary Epithelial Cells *
    Article Snippet: Here, we have demonstrated that BTG2 mediates protection of human mammary epithelial cells against oxidative stress due to H2 O2 and other oxidizing agents.

    Infection:

    Article Title: The B Cell Translocation Gene (BTG) Family in the Rat Ovary: Hormonal Induction, Regulation, and Impact on Cell Cycle Kinetics
    Article Snippet: Treatment with Ad-DNRUNX inhibited Btg2 mRNA expression stimulated by FSK plus PMA treatment (Fig. 5D ), which suggests that RUNX is involved in Btg2 transcriptional regulation. .. To investigate the potential function of BTG1 and BTG2, granulosa cells from preovulatory ovaries were infected with either a recombinant Ad-BTG1 or Ad-BTG2 adenovirus. .. Granulosa cells infected with Ad-GFP were used as a control.

    Recombinant:

    Article Title: The B Cell Translocation Gene (BTG) Family in the Rat Ovary: Hormonal Induction, Regulation, and Impact on Cell Cycle Kinetics
    Article Snippet: Treatment with Ad-DNRUNX inhibited Btg2 mRNA expression stimulated by FSK plus PMA treatment (Fig. 5D ), which suggests that RUNX is involved in Btg2 transcriptional regulation. .. To investigate the potential function of BTG1 and BTG2, granulosa cells from preovulatory ovaries were infected with either a recombinant Ad-BTG1 or Ad-BTG2 adenovirus. .. Granulosa cells infected with Ad-GFP were used as a control.

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    <t>BTG2</t> protects against oxidative stress. A and B , MCF-7 cells were transfected overnight with wtBTG2 or empty pcDNA3 vector; harvested using trypsin; seeded into 96-well dishes; allowed to attach; exposed to different concentrations of H 2 O 2 for 24 h; and
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    BTG2 protects against oxidative stress. A and B , MCF-7 cells were transfected overnight with wtBTG2 or empty pcDNA3 vector; harvested using trypsin; seeded into 96-well dishes; allowed to attach; exposed to different concentrations of H 2 O 2 for 24 h; and

    Journal: The Journal of Biological Chemistry

    Article Title: B-cell Translocation Gene 2 (BTG2) Stimulates Cellular Antioxidant Defenses through the Antioxidant Transcription Factor NFE2L2 in Human Mammary Epithelial Cells *

    doi: 10.1074/jbc.M112.367433

    Figure Lengend Snippet: BTG2 protects against oxidative stress. A and B , MCF-7 cells were transfected overnight with wtBTG2 or empty pcDNA3 vector; harvested using trypsin; seeded into 96-well dishes; allowed to attach; exposed to different concentrations of H 2 O 2 for 24 h; and

    Article Snippet: BTG2 also binds to and modulates the activity of PRMT1, a histone H4-specific methyltransferase that participates in a variety of transcriptional pathways ( ).

    Techniques: Transfection, Plasmid Preparation

    BTG2 regulates NFE2L2/ARE signaling. MCF-7 cells were co-transfected with NQO1-ARE-Luc reporter and the indicated expression vector(s); postincubated for 24 h; and harvested for luciferase assays. The total transfected DNA content was kept constant by

    Journal: The Journal of Biological Chemistry

    Article Title: B-cell Translocation Gene 2 (BTG2) Stimulates Cellular Antioxidant Defenses through the Antioxidant Transcription Factor NFE2L2 in Human Mammary Epithelial Cells *

    doi: 10.1074/jbc.M112.367433

    Figure Lengend Snippet: BTG2 regulates NFE2L2/ARE signaling. MCF-7 cells were co-transfected with NQO1-ARE-Luc reporter and the indicated expression vector(s); postincubated for 24 h; and harvested for luciferase assays. The total transfected DNA content was kept constant by

    Article Snippet: BTG2 also binds to and modulates the activity of PRMT1, a histone H4-specific methyltransferase that participates in a variety of transcriptional pathways ( ).

    Techniques: Transfection, Expressing, Plasmid Preparation, Luciferase

    BTG2 physically associates with NFE2L2. A and B , MCF-7 cells were transfected with FLAG-BTG2 or empty pCMV6-FLAG vector; postincubated for 24 h; and subjected to anti-FLAG ( A ) or anti-NFE2L2 ( B ) IP. Precipitated proteins were Western blotted using anti-FLAG

    Journal: The Journal of Biological Chemistry

    Article Title: B-cell Translocation Gene 2 (BTG2) Stimulates Cellular Antioxidant Defenses through the Antioxidant Transcription Factor NFE2L2 in Human Mammary Epithelial Cells *

    doi: 10.1074/jbc.M112.367433

    Figure Lengend Snippet: BTG2 physically associates with NFE2L2. A and B , MCF-7 cells were transfected with FLAG-BTG2 or empty pCMV6-FLAG vector; postincubated for 24 h; and subjected to anti-FLAG ( A ) or anti-NFE2L2 ( B ) IP. Precipitated proteins were Western blotted using anti-FLAG

    Article Snippet: BTG2 also binds to and modulates the activity of PRMT1, a histone H4-specific methyltransferase that participates in a variety of transcriptional pathways ( ).

    Techniques: Transfection, Plasmid Preparation, Western Blot

    BTG2 up-regulates expression of antioxidant enzymes. A and B , MCF-7 cells were transfected overnight with wtBTG2 or pcDNA3 vector; postincubated for 24 h to allow gene expression; harvested; and subjected to semiquantitative RT-PCR ( A ) or Western blot

    Journal: The Journal of Biological Chemistry

    Article Title: B-cell Translocation Gene 2 (BTG2) Stimulates Cellular Antioxidant Defenses through the Antioxidant Transcription Factor NFE2L2 in Human Mammary Epithelial Cells *

    doi: 10.1074/jbc.M112.367433

    Figure Lengend Snippet: BTG2 up-regulates expression of antioxidant enzymes. A and B , MCF-7 cells were transfected overnight with wtBTG2 or pcDNA3 vector; postincubated for 24 h to allow gene expression; harvested; and subjected to semiquantitative RT-PCR ( A ) or Western blot

    Article Snippet: BTG2 also binds to and modulates the activity of PRMT1, a histone H4-specific methyltransferase that participates in a variety of transcriptional pathways ( ).

    Techniques: Expressing, Transfection, Plasmid Preparation, Reverse Transcription Polymerase Chain Reaction, Western Blot

    BTG2 induces growth arrest of H 2 O 2 -treated cells. A , MCF-7 cells were transfected with wtBTG2 or pcDNA3 vector and treated with different concentrations of H 2 O 2 for 24 h, with exposure to BrdU during the last 12 h of H 2 O 2 treatment. The percentage of

    Journal: The Journal of Biological Chemistry

    Article Title: B-cell Translocation Gene 2 (BTG2) Stimulates Cellular Antioxidant Defenses through the Antioxidant Transcription Factor NFE2L2 in Human Mammary Epithelial Cells *

    doi: 10.1074/jbc.M112.367433

    Figure Lengend Snippet: BTG2 induces growth arrest of H 2 O 2 -treated cells. A , MCF-7 cells were transfected with wtBTG2 or pcDNA3 vector and treated with different concentrations of H 2 O 2 for 24 h, with exposure to BrdU during the last 12 h of H 2 O 2 treatment. The percentage of

    Article Snippet: BTG2 also binds to and modulates the activity of PRMT1, a histone H4-specific methyltransferase that participates in a variety of transcriptional pathways ( ).

    Techniques: Transfection, Plasmid Preparation

    Box B of BTG2 is required for association of BTG2 with NFE2L2 and for BTG2-mediated protection against oxidative stress in MCF-7 cells. A , schematic diagrams showing the structures of the proteins encoded by the wt-HA-BTG2, HA-BTG2-ΔA, HA-BTG2-ΔB,

    Journal: The Journal of Biological Chemistry

    Article Title: B-cell Translocation Gene 2 (BTG2) Stimulates Cellular Antioxidant Defenses through the Antioxidant Transcription Factor NFE2L2 in Human Mammary Epithelial Cells *

    doi: 10.1074/jbc.M112.367433

    Figure Lengend Snippet: Box B of BTG2 is required for association of BTG2 with NFE2L2 and for BTG2-mediated protection against oxidative stress in MCF-7 cells. A , schematic diagrams showing the structures of the proteins encoded by the wt-HA-BTG2, HA-BTG2-ΔA, HA-BTG2-ΔB,

    Article Snippet: BTG2 also binds to and modulates the activity of PRMT1, a histone H4-specific methyltransferase that participates in a variety of transcriptional pathways ( ).

    Techniques:

    BTG2 and NFE2L2 are present at the ARE. A , untransfected MCF-7 cells were subjected to ChIP assays using anti-NFE2L2 antibody or nonimmune rabbit IgG (negative control). The PCR primers correspond to two different AREs (E1 and E2) of the HO-1 gene, HO-1

    Journal: The Journal of Biological Chemistry

    Article Title: B-cell Translocation Gene 2 (BTG2) Stimulates Cellular Antioxidant Defenses through the Antioxidant Transcription Factor NFE2L2 in Human Mammary Epithelial Cells *

    doi: 10.1074/jbc.M112.367433

    Figure Lengend Snippet: BTG2 and NFE2L2 are present at the ARE. A , untransfected MCF-7 cells were subjected to ChIP assays using anti-NFE2L2 antibody or nonimmune rabbit IgG (negative control). The PCR primers correspond to two different AREs (E1 and E2) of the HO-1 gene, HO-1

    Article Snippet: BTG2 also binds to and modulates the activity of PRMT1, a histone H4-specific methyltransferase that participates in a variety of transcriptional pathways ( ).

    Techniques: Chromatin Immunoprecipitation, Negative Control, Polymerase Chain Reaction

    BTG2 regulates antioxidant enzyme activity and cellular redox state. A and B , MCF-7 cells were transfected overnight with wtBTG2 or pcDNA3 vector; postincubated for 24 h; and assayed to determine catalase ( A ) or total SOD ( B ) activity. C and D , 184A1

    Journal: The Journal of Biological Chemistry

    Article Title: B-cell Translocation Gene 2 (BTG2) Stimulates Cellular Antioxidant Defenses through the Antioxidant Transcription Factor NFE2L2 in Human Mammary Epithelial Cells *

    doi: 10.1074/jbc.M112.367433

    Figure Lengend Snippet: BTG2 regulates antioxidant enzyme activity and cellular redox state. A and B , MCF-7 cells were transfected overnight with wtBTG2 or pcDNA3 vector; postincubated for 24 h; and assayed to determine catalase ( A ) or total SOD ( B ) activity. C and D , 184A1

    Article Snippet: BTG2 also binds to and modulates the activity of PRMT1, a histone H4-specific methyltransferase that participates in a variety of transcriptional pathways ( ).

    Techniques: Activity Assay, Transfection, Plasmid Preparation