Structured Review

Thermo Fisher bsrgi
Bsrgi, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/bsrgi/product/Thermo Fisher
Average 91 stars, based on 11 article reviews
Price from $9.99 to $1999.99
bsrgi - by Bioz Stars, 2020-09
91/100 stars

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Related Articles

Clone Assay:

Article Title: TRIM25 Enhances the Antiviral Action of Zinc-Finger Antiviral Protein (ZAP)
Article Snippet: .. ZAPS and ZAPL were amplified from 293T cDNA using primers (ZAPS: 5’-GTTTTGTACAGCCACCATGGCGGACCCGGAGGTG-3’ and 5’-GGTAGCGGCCGCTTACTCTGGCCCTCTCTTCATC-3’; ZAPL: 5’-GTTTTGTACAGCCACCATGGCGGACCCGGAGGTG-3’ and 5’-GGTA GCGGCCGCCTAACTAATCACGCAGGCTTTG-3’) and cloned into the BsrGI and NotI sites of a modified pTRIPZ construct (Open Biosystems) under the control of the CMV promoter. .. The 3’ ends of ZAPS and ZAPL were swapped into SmaI and XhoI sites of pTRIP-RFP-NZAP [ ] to generate pTRIP constructs expressing N-terminally RFP-fused ZAPS and ZAPL.

Article Title: Characterization of Novel Splice Variants of Zinc Finger Antiviral Protein (ZAP)
Article Snippet: .. The resultant PCR products were digested and cloned into the BsrGI and NotI sites of a modified pTRIPZ construct (Open Biosystems) under the control of the cytomegalovirus (CMV) promoter to generate constructs expressing untagged ZAPM and ZAPXL (pTRIPZ-ZAPM and pTRIPZ-ZAPXL). .. The 3′ ends of ZAPM and ZAPXL, including the extended exon 4, were digested out of the pTRIPZ-ZAPM and pTRIPZ-ZAPXL plasmids using SmaI (present in the ZAP gene) and XhoI (present in the pTRIPZ vector downstream of ZAP).

Amplification:

Article Title: TRIM25 Enhances the Antiviral Action of Zinc-Finger Antiviral Protein (ZAP)
Article Snippet: .. ZAPS and ZAPL were amplified from 293T cDNA using primers (ZAPS: 5’-GTTTTGTACAGCCACCATGGCGGACCCGGAGGTG-3’ and 5’-GGTAGCGGCCGCTTACTCTGGCCCTCTCTTCATC-3’; ZAPL: 5’-GTTTTGTACAGCCACCATGGCGGACCCGGAGGTG-3’ and 5’-GGTA GCGGCCGCCTAACTAATCACGCAGGCTTTG-3’) and cloned into the BsrGI and NotI sites of a modified pTRIPZ construct (Open Biosystems) under the control of the CMV promoter. .. The 3’ ends of ZAPS and ZAPL were swapped into SmaI and XhoI sites of pTRIP-RFP-NZAP [ ] to generate pTRIP constructs expressing N-terminally RFP-fused ZAPS and ZAPL.

Plasmid Preparation:

Article Title: Morphology of Axonal Projections From the High Vocal Center to Vocal Motor Cortex in Songbirds
Article Snippet: .. This vector was produced by removing the CAG-GFP cassette from pCAG-GFP ( ; Addgene plasmid 11150) using SpeI and BsrGI, and subcloning this cassette into the lentiviral backbone, derived from FCK-ChR2-GFP (Addgene plasmid 15814; ) cut with XbaI and BsrGI (all enzymes from Fermentas, Burlington, ON, Canada). .. The lentiviral vector construct was verified by enzymatic digestion, gel electrophoresis, and DNA sequencing (DNA Core Facility, USC).

Article Title: Metastatic cancers promote cachexia through altered zinc homeostasis in skeletal muscle
Article Snippet: .. AAV-CAG-ChR2-GFP was also used as backbone AAV vector with CAG promoter after digesting by BamHI (Roche) and BsrGI (Thermo Fisher). .. By sequential PCR amplification, mCherry alone, Zip14-IRES-GFP amplicons, containing N-terminal BamHI and C-terminal BsrGI digestion sites, were introduced into digested AAV-CAG backbone to get the AAV vectors.

Ligation:

Article Title: mll ortholog containing functional domains of human MLL is expressed throughout the zebrafish lifespan and in haematopoietic tissues
Article Snippet: .. A full-length ORF cDNA subclone from 24 hpf embryos was obtained by restriction enzyme cleavage of the 5’UTR-exon 3 PCR product with NotI and BsrGI, of the larger central exon 1–35 subclone with BsrGI and ClaI, and of the exon 33–35 PCR product with ClaI and KpnI, followed by ligation of the 5’ and 3’ fragments to the BsrGI or ClaI sites in the larger central fragment and to NotI and KpnI sites in pCR-XL-TOPO (Invitrogen). .. A cDNA spanning the 5’UTR through the 3’UTR was generated from random hexamer primed cDNAs by long-distance RT-PCR using AccuPrime™ High Fidelity Taq DNA Polymerase (Invitrogen), subcloned into pCR-XL-TOPO (Invitrogen) and sequenced.

Subcloning:

Article Title: Morphology of Axonal Projections From the High Vocal Center to Vocal Motor Cortex in Songbirds
Article Snippet: .. This vector was produced by removing the CAG-GFP cassette from pCAG-GFP ( ; Addgene plasmid 11150) using SpeI and BsrGI, and subcloning this cassette into the lentiviral backbone, derived from FCK-ChR2-GFP (Addgene plasmid 15814; ) cut with XbaI and BsrGI (all enzymes from Fermentas, Burlington, ON, Canada). .. The lentiviral vector construct was verified by enzymatic digestion, gel electrophoresis, and DNA sequencing (DNA Core Facility, USC).

Construct:

Article Title: TRIM25 Enhances the Antiviral Action of Zinc-Finger Antiviral Protein (ZAP)
Article Snippet: .. ZAPS and ZAPL were amplified from 293T cDNA using primers (ZAPS: 5’-GTTTTGTACAGCCACCATGGCGGACCCGGAGGTG-3’ and 5’-GGTAGCGGCCGCTTACTCTGGCCCTCTCTTCATC-3’; ZAPL: 5’-GTTTTGTACAGCCACCATGGCGGACCCGGAGGTG-3’ and 5’-GGTA GCGGCCGCCTAACTAATCACGCAGGCTTTG-3’) and cloned into the BsrGI and NotI sites of a modified pTRIPZ construct (Open Biosystems) under the control of the CMV promoter. .. The 3’ ends of ZAPS and ZAPL were swapped into SmaI and XhoI sites of pTRIP-RFP-NZAP [ ] to generate pTRIP constructs expressing N-terminally RFP-fused ZAPS and ZAPL.

Article Title: STAT1β Is Not Dominant Negative and Is Capable of Contributing to Gamma Interferon-Dependent Innate Immunity
Article Snippet: .. Homologously integrated constructs were screened by detection of a 7.4-kb (wild-type [WT]), a 5.7-kb ( Stat1 α), and a 5.6-kb ( Stat1 β) long fragment after digestion with BsrGI by using a 5′ [α-32 P]ATP-labeled probe (Random labeling kit; Invitrogen) obtained by PCR with the following primers: forward, 5′-ACAAAGCCACCATTCGTAGG-3′; reverse, 5′-GTGCATCTCTGAGCAAAACC-3′. .. Isolated DNAs from ES cell clones were used as templates.

Article Title: Characterization of Novel Splice Variants of Zinc Finger Antiviral Protein (ZAP)
Article Snippet: .. The resultant PCR products were digested and cloned into the BsrGI and NotI sites of a modified pTRIPZ construct (Open Biosystems) under the control of the cytomegalovirus (CMV) promoter to generate constructs expressing untagged ZAPM and ZAPXL (pTRIPZ-ZAPM and pTRIPZ-ZAPXL). .. The 3′ ends of ZAPM and ZAPXL, including the extended exon 4, were digested out of the pTRIPZ-ZAPM and pTRIPZ-ZAPXL plasmids using SmaI (present in the ZAP gene) and XhoI (present in the pTRIPZ vector downstream of ZAP).

Produced:

Article Title: Morphology of Axonal Projections From the High Vocal Center to Vocal Motor Cortex in Songbirds
Article Snippet: .. This vector was produced by removing the CAG-GFP cassette from pCAG-GFP ( ; Addgene plasmid 11150) using SpeI and BsrGI, and subcloning this cassette into the lentiviral backbone, derived from FCK-ChR2-GFP (Addgene plasmid 15814; ) cut with XbaI and BsrGI (all enzymes from Fermentas, Burlington, ON, Canada). .. The lentiviral vector construct was verified by enzymatic digestion, gel electrophoresis, and DNA sequencing (DNA Core Facility, USC).

Modification:

Article Title: TRIM25 Enhances the Antiviral Action of Zinc-Finger Antiviral Protein (ZAP)
Article Snippet: .. ZAPS and ZAPL were amplified from 293T cDNA using primers (ZAPS: 5’-GTTTTGTACAGCCACCATGGCGGACCCGGAGGTG-3’ and 5’-GGTAGCGGCCGCTTACTCTGGCCCTCTCTTCATC-3’; ZAPL: 5’-GTTTTGTACAGCCACCATGGCGGACCCGGAGGTG-3’ and 5’-GGTA GCGGCCGCCTAACTAATCACGCAGGCTTTG-3’) and cloned into the BsrGI and NotI sites of a modified pTRIPZ construct (Open Biosystems) under the control of the CMV promoter. .. The 3’ ends of ZAPS and ZAPL were swapped into SmaI and XhoI sites of pTRIP-RFP-NZAP [ ] to generate pTRIP constructs expressing N-terminally RFP-fused ZAPS and ZAPL.

Article Title: Characterization of Novel Splice Variants of Zinc Finger Antiviral Protein (ZAP)
Article Snippet: .. The resultant PCR products were digested and cloned into the BsrGI and NotI sites of a modified pTRIPZ construct (Open Biosystems) under the control of the cytomegalovirus (CMV) promoter to generate constructs expressing untagged ZAPM and ZAPXL (pTRIPZ-ZAPM and pTRIPZ-ZAPXL). .. The 3′ ends of ZAPM and ZAPXL, including the extended exon 4, were digested out of the pTRIPZ-ZAPM and pTRIPZ-ZAPXL plasmids using SmaI (present in the ZAP gene) and XhoI (present in the pTRIPZ vector downstream of ZAP).

Labeling:

Article Title: STAT1β Is Not Dominant Negative and Is Capable of Contributing to Gamma Interferon-Dependent Innate Immunity
Article Snippet: .. Homologously integrated constructs were screened by detection of a 7.4-kb (wild-type [WT]), a 5.7-kb ( Stat1 α), and a 5.6-kb ( Stat1 β) long fragment after digestion with BsrGI by using a 5′ [α-32 P]ATP-labeled probe (Random labeling kit; Invitrogen) obtained by PCR with the following primers: forward, 5′-ACAAAGCCACCATTCGTAGG-3′; reverse, 5′-GTGCATCTCTGAGCAAAACC-3′. .. Isolated DNAs from ES cell clones were used as templates.

Expressing:

Article Title: Characterization of Novel Splice Variants of Zinc Finger Antiviral Protein (ZAP)
Article Snippet: .. The resultant PCR products were digested and cloned into the BsrGI and NotI sites of a modified pTRIPZ construct (Open Biosystems) under the control of the cytomegalovirus (CMV) promoter to generate constructs expressing untagged ZAPM and ZAPXL (pTRIPZ-ZAPM and pTRIPZ-ZAPXL). .. The 3′ ends of ZAPM and ZAPXL, including the extended exon 4, were digested out of the pTRIPZ-ZAPM and pTRIPZ-ZAPXL plasmids using SmaI (present in the ZAP gene) and XhoI (present in the pTRIPZ vector downstream of ZAP).

Polymerase Chain Reaction:

Article Title: mll ortholog containing functional domains of human MLL is expressed throughout the zebrafish lifespan and in haematopoietic tissues
Article Snippet: .. A full-length ORF cDNA subclone from 24 hpf embryos was obtained by restriction enzyme cleavage of the 5’UTR-exon 3 PCR product with NotI and BsrGI, of the larger central exon 1–35 subclone with BsrGI and ClaI, and of the exon 33–35 PCR product with ClaI and KpnI, followed by ligation of the 5’ and 3’ fragments to the BsrGI or ClaI sites in the larger central fragment and to NotI and KpnI sites in pCR-XL-TOPO (Invitrogen). .. A cDNA spanning the 5’UTR through the 3’UTR was generated from random hexamer primed cDNAs by long-distance RT-PCR using AccuPrime™ High Fidelity Taq DNA Polymerase (Invitrogen), subcloned into pCR-XL-TOPO (Invitrogen) and sequenced.

Article Title: STAT1β Is Not Dominant Negative and Is Capable of Contributing to Gamma Interferon-Dependent Innate Immunity
Article Snippet: .. Homologously integrated constructs were screened by detection of a 7.4-kb (wild-type [WT]), a 5.7-kb ( Stat1 α), and a 5.6-kb ( Stat1 β) long fragment after digestion with BsrGI by using a 5′ [α-32 P]ATP-labeled probe (Random labeling kit; Invitrogen) obtained by PCR with the following primers: forward, 5′-ACAAAGCCACCATTCGTAGG-3′; reverse, 5′-GTGCATCTCTGAGCAAAACC-3′. .. Isolated DNAs from ES cell clones were used as templates.

Article Title: Characterization of Novel Splice Variants of Zinc Finger Antiviral Protein (ZAP)
Article Snippet: .. The resultant PCR products were digested and cloned into the BsrGI and NotI sites of a modified pTRIPZ construct (Open Biosystems) under the control of the cytomegalovirus (CMV) promoter to generate constructs expressing untagged ZAPM and ZAPXL (pTRIPZ-ZAPM and pTRIPZ-ZAPXL). .. The 3′ ends of ZAPM and ZAPXL, including the extended exon 4, were digested out of the pTRIPZ-ZAPM and pTRIPZ-ZAPXL plasmids using SmaI (present in the ZAP gene) and XhoI (present in the pTRIPZ vector downstream of ZAP).

Derivative Assay:

Article Title: Morphology of Axonal Projections From the High Vocal Center to Vocal Motor Cortex in Songbirds
Article Snippet: .. This vector was produced by removing the CAG-GFP cassette from pCAG-GFP ( ; Addgene plasmid 11150) using SpeI and BsrGI, and subcloning this cassette into the lentiviral backbone, derived from FCK-ChR2-GFP (Addgene plasmid 15814; ) cut with XbaI and BsrGI (all enzymes from Fermentas, Burlington, ON, Canada). .. The lentiviral vector construct was verified by enzymatic digestion, gel electrophoresis, and DNA sequencing (DNA Core Facility, USC).

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