bsrgi hf  (New England Biolabs)


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  • 99
    Name:
    BsrGI HF
    Description:
    BsrGI HF 5 000 units
    Catalog Number:
    r3575l
    Price:
    282
    Size:
    5 000 units
    Category:
    Restriction Enzymes
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    Structured Review

    New England Biolabs bsrgi hf
    BsrGI HF
    BsrGI HF 5 000 units
    https://www.bioz.com/result/bsrgi hf/product/New England Biolabs
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    bsrgi hf - by Bioz Stars, 2020-04
    99/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Deep mutational scanning of S. pyogenes Cas9 reveals important functional domains
    Article Snippet: Paragraph title: Specific SpCas9 Mutant Cloning ... The fused PCR products were either digested with BstXI and BsrGI-HF (NEB) and ligated back into the mammalian expression vector EF-SpCas9 (Supplementary Figure ) for mammalian expression or used for golden gate assembly into pUC-ProD-LacZ-T2 for bacterial expression as described above.

    Article Title: The dTAG system for immediate and target-specific protein degradation
    Article Snippet: Plasmid DNA was purified using midi-preps (Qiagen) and insertions were confirmed by BsrGI-HF digestion (NEB) and gel electrophoresis. .. Note that when designing plasmids for Gateway recombination cloning into the N-dTAG vector, a stop codon must be included at the end of the gene to prevent read-through into the recombination sequences. lists plasmids associated with the dTAG system.

    shRNA:

    Article Title: Dual RNA-Seq Unveils the Role of the Pseudomonas plecoglossicida fliA Gene in Pathogen-Host Interaction with Larimichthys crocea
    Article Snippet: .. First, the pCM130/tac vector was linearized using the restriction enzymes NsiI-HF and BsrGI-HF (New England Biolabs, Ipswich, MA, USA) and ligated to annealed shRNA with T4 DNA ligase. .. Second, the recombinant plasmid was transformed into E. coli DH5a by heat shock, extracted using EasyPure Plasmid MiniPrep Kit (TransGen Biotech, Beijing, China) and electroporated into P. plecoglossicida .

    Nucleic Acid Electrophoresis:

    Article Title: The dTAG system for immediate and target-specific protein degradation
    Article Snippet: .. Plasmid DNA was purified using midi-preps (Qiagen) and insertions were confirmed by BsrGI-HF digestion (NEB) and gel electrophoresis. .. Note that when designing plasmids for Gateway recombination cloning into the N-dTAG vector, a stop codon must be included at the end of the gene to prevent read-through into the recombination sequences. lists plasmids associated with the dTAG system.

    Mutagenesis:

    Article Title: Deep mutational scanning of S. pyogenes Cas9 reveals important functional domains
    Article Snippet: Paragraph title: Specific SpCas9 Mutant Cloning ... The fused PCR products were either digested with BstXI and BsrGI-HF (NEB) and ligated back into the mammalian expression vector EF-SpCas9 (Supplementary Figure ) for mammalian expression or used for golden gate assembly into pUC-ProD-LacZ-T2 for bacterial expression as described above.

    Cell Culture:

    Article Title: Dual RNA-Seq Unveils the Role of the Pseudomonas plecoglossicida fliA Gene in Pathogen-Host Interaction with Larimichthys crocea
    Article Snippet: First, the pCM130/tac vector was linearized using the restriction enzymes NsiI-HF and BsrGI-HF (New England Biolabs, Ipswich, MA, USA) and ligated to annealed shRNA with T4 DNA ligase. .. Each strain was cultured to an OD600 0.5 ± 0.1, and the level of fliA mRNA was detected by quantitative real-time (qRT)-PCR for three independent experiments.

    Construct:

    Article Title: Dual RNA-Seq Unveils the Role of the Pseudomonas plecoglossicida fliA Gene in Pathogen-Host Interaction with Larimichthys crocea
    Article Snippet: Construction of the RNAi Strain pCM130/tac [ ] was used to construct the P. plecoglossicida RNAi strain according to a previously described method [ , ]. .. First, the pCM130/tac vector was linearized using the restriction enzymes NsiI-HF and BsrGI-HF (New England Biolabs, Ipswich, MA, USA) and ligated to annealed shRNA with T4 DNA ligase.

    Purification:

    Article Title: The dTAG system for immediate and target-specific protein degradation
    Article Snippet: .. Plasmid DNA was purified using midi-preps (Qiagen) and insertions were confirmed by BsrGI-HF digestion (NEB) and gel electrophoresis. .. Note that when designing plasmids for Gateway recombination cloning into the N-dTAG vector, a stop codon must be included at the end of the gene to prevent read-through into the recombination sequences. lists plasmids associated with the dTAG system.

    Plasmid Preparation:

    Article Title: Deep mutational scanning of S. pyogenes Cas9 reveals important functional domains
    Article Snippet: .. The fused PCR products were either digested with BstXI and BsrGI-HF (NEB) and ligated back into the mammalian expression vector EF-SpCas9 (Supplementary Figure ) for mammalian expression or used for golden gate assembly into pUC-ProD-LacZ-T2 for bacterial expression as described above. .. All primers used for mutation PCRs can be found in Supplementary Table .

    Article Title: The dTAG system for immediate and target-specific protein degradation
    Article Snippet: .. Plasmid DNA was purified using midi-preps (Qiagen) and insertions were confirmed by BsrGI-HF digestion (NEB) and gel electrophoresis. .. Note that when designing plasmids for Gateway recombination cloning into the N-dTAG vector, a stop codon must be included at the end of the gene to prevent read-through into the recombination sequences. lists plasmids associated with the dTAG system.

    Article Title: Dual RNA-Seq Unveils the Role of the Pseudomonas plecoglossicida fliA Gene in Pathogen-Host Interaction with Larimichthys crocea
    Article Snippet: .. First, the pCM130/tac vector was linearized using the restriction enzymes NsiI-HF and BsrGI-HF (New England Biolabs, Ipswich, MA, USA) and ligated to annealed shRNA with T4 DNA ligase. .. Second, the recombinant plasmid was transformed into E. coli DH5a by heat shock, extracted using EasyPure Plasmid MiniPrep Kit (TransGen Biotech, Beijing, China) and electroporated into P. plecoglossicida .

    Article Title: Compartmentalized partnered replication for the directed evolution of genetic parts and circuits
    Article Snippet: .. 11B.AS3.4 encoding S. cerevisiae suppressor tryptophanyl tRNA or a derivative with inactive tRNA (for basic plasmid maps, see ; available upon request from the Ellington group or Addgene (cat. no. 102789)) Carbenicillin disodium salt (GoldBio, cat. no. C-103) 5-hydroxy-L-tryptophan powder (5HTP; Sigma-Aldrich, cat. no. H9772) Restriction enzyme KpnI-HF (New England BioLabs, cat. no. R3142) Restriction enzyme BsrGI-HF (New England BioLabs, cat. no. R3575) BL21(DE3) E. coli strain carrying the pACYC.Taq. ..

    Incubation:

    Article Title: The dTAG system for immediate and target-specific protein degradation
    Article Snippet: To terminate the reaction, 1 µl of Proteinase K was added to each sample and incubated at 37 ºC for 10 minutes. .. Plasmid DNA was purified using midi-preps (Qiagen) and insertions were confirmed by BsrGI-HF digestion (NEB) and gel electrophoresis.

    Selection:

    Article Title: Compartmentalized partnered replication for the directed evolution of genetic parts and circuits
    Article Snippet: Paragraph title: Additional reagents for tRNA selection ... 11B.AS3.4 encoding S. cerevisiae suppressor tryptophanyl tRNA or a derivative with inactive tRNA (for basic plasmid maps, see ; available upon request from the Ellington group or Addgene (cat. no. 102789)) Carbenicillin disodium salt (GoldBio, cat. no. C-103) 5-hydroxy-L-tryptophan powder (5HTP; Sigma-Aldrich, cat. no. H9772) Restriction enzyme KpnI-HF (New England BioLabs, cat. no. R3142) Restriction enzyme BsrGI-HF (New England BioLabs, cat. no. R3575) BL21(DE3) E. coli strain carrying the pACYC.Taq.

    Quantitative RT-PCR:

    Article Title: Dual RNA-Seq Unveils the Role of the Pseudomonas plecoglossicida fliA Gene in Pathogen-Host Interaction with Larimichthys crocea
    Article Snippet: First, the pCM130/tac vector was linearized using the restriction enzymes NsiI-HF and BsrGI-HF (New England Biolabs, Ipswich, MA, USA) and ligated to annealed shRNA with T4 DNA ligase. .. Each strain was cultured to an OD600 0.5 ± 0.1, and the level of fliA mRNA was detected by quantitative real-time (qRT)-PCR for three independent experiments.

    Expressing:

    Article Title: Deep mutational scanning of S. pyogenes Cas9 reveals important functional domains
    Article Snippet: .. The fused PCR products were either digested with BstXI and BsrGI-HF (NEB) and ligated back into the mammalian expression vector EF-SpCas9 (Supplementary Figure ) for mammalian expression or used for golden gate assembly into pUC-ProD-LacZ-T2 for bacterial expression as described above. .. All primers used for mutation PCRs can be found in Supplementary Table .

    Polymerase Chain Reaction:

    Article Title: Deep mutational scanning of S. pyogenes Cas9 reveals important functional domains
    Article Snippet: .. The fused PCR products were either digested with BstXI and BsrGI-HF (NEB) and ligated back into the mammalian expression vector EF-SpCas9 (Supplementary Figure ) for mammalian expression or used for golden gate assembly into pUC-ProD-LacZ-T2 for bacterial expression as described above. .. All primers used for mutation PCRs can be found in Supplementary Table .

    Article Title: Real-time fluorescence loop-mediated isothermal amplification assay for direct detection of egg drop syndrome virus
    Article Snippet: Enzymes including Bst2.0 DNA polymerase (8000 U/ml) and BsrGI-HF (20,000 U/ml) were obtained from New England Bio labs (NEB, USA). .. Primers for RealAmp and PCR (oligonucleotides) were obtained from Huada (Beijing, China) and suspended in deionized water with appropriate concentrations and stored at − 20 °C.

    Article Title: Real-time fluorescence loop-mediated isothermal amplification assay for direct detection of egg drop syndrome virus
    Article Snippet: Chemicals and reagents Enzymes including Bst2.0 DNA polymerase (8000 U/ml) and BsrGI-HF (20,000 U/ml) were obtained from New England Bio labs (NEB, USA). .. Primers for RealAmp and PCR (oligonucleotides) were obtained from Huada (Beijing, China) and suspended in deionized water with appropriate concentrations and stored at − 20 °C.

    Recombinant:

    Article Title: Dual RNA-Seq Unveils the Role of the Pseudomonas plecoglossicida fliA Gene in Pathogen-Host Interaction with Larimichthys crocea
    Article Snippet: First, the pCM130/tac vector was linearized using the restriction enzymes NsiI-HF and BsrGI-HF (New England Biolabs, Ipswich, MA, USA) and ligated to annealed shRNA with T4 DNA ligase. .. Second, the recombinant plasmid was transformed into E. coli DH5a by heat shock, extracted using EasyPure Plasmid MiniPrep Kit (TransGen Biotech, Beijing, China) and electroporated into P. plecoglossicida .

    Transformation Assay:

    Article Title: The dTAG system for immediate and target-specific protein degradation
    Article Snippet: Samples were transformed using Stbl3 competent cells, plated on Ampicillin selective media, and grown at 30 ºC. .. Plasmid DNA was purified using midi-preps (Qiagen) and insertions were confirmed by BsrGI-HF digestion (NEB) and gel electrophoresis.

    Article Title: Dual RNA-Seq Unveils the Role of the Pseudomonas plecoglossicida fliA Gene in Pathogen-Host Interaction with Larimichthys crocea
    Article Snippet: First, the pCM130/tac vector was linearized using the restriction enzymes NsiI-HF and BsrGI-HF (New England Biolabs, Ipswich, MA, USA) and ligated to annealed shRNA with T4 DNA ligase. .. Second, the recombinant plasmid was transformed into E. coli DH5a by heat shock, extracted using EasyPure Plasmid MiniPrep Kit (TransGen Biotech, Beijing, China) and electroporated into P. plecoglossicida .

    Spectrophotometry:

    Article Title: Real-time fluorescence loop-mediated isothermal amplification assay for direct detection of egg drop syndrome virus
    Article Snippet: Enzymes including Bst2.0 DNA polymerase (8000 U/ml) and BsrGI-HF (20,000 U/ml) were obtained from New England Bio labs (NEB, USA). .. The concentrations of each DNA suspension used in this study were measured by NanoVue Plus spectrophotometer (GE Healthcare, USA).

    Article Title: Real-time fluorescence loop-mediated isothermal amplification assay for direct detection of egg drop syndrome virus
    Article Snippet: Chemicals and reagents Enzymes including Bst2.0 DNA polymerase (8000 U/ml) and BsrGI-HF (20,000 U/ml) were obtained from New England Bio labs (NEB, USA). .. The concentrations of each DNA suspension used in this study were measured by NanoVue Plus spectrophotometer (GE Healthcare, USA).

    Blocking Assay:

    Article Title: Dual RNA-Seq Unveils the Role of the Pseudomonas plecoglossicida fliA Gene in Pathogen-Host Interaction with Larimichthys crocea
    Article Snippet: Five siRNA sequences targeting the fliA gene were designed by BLOCK-iTTM RNAi Designer ( http://rnaidesigner.thermofisher.com/rnaiexpress/setOption.do?designOption=shrna & pid=708587103220684543 ) and synthetized by Shanghai Generay Biotech Co., Ltd. (Shanghai, China; ). .. First, the pCM130/tac vector was linearized using the restriction enzymes NsiI-HF and BsrGI-HF (New England Biolabs, Ipswich, MA, USA) and ligated to annealed shRNA with T4 DNA ligase.

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  • 99
    New England Biolabs restriction enzyme bsrgi hf
    Restriction Enzyme Bsrgi Hf, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/restriction enzyme bsrgi hf/product/New England Biolabs
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    restriction enzyme bsrgi hf - by Bioz Stars, 2020-04
    99/100 stars
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