Structured Review

Roche bspei
Bspei, supplied by Roche, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/bspei/product/Roche
Average 90 stars, based on 2 article reviews
Price from $9.99 to $1999.99
bspei - by Bioz Stars, 2020-07
90/100 stars

Images

Related Articles

Sequencing:

Article Title: Optimizing microarray-based in situ transcription-translation of proteins for MALDI mass spectrometry
Article Snippet: .. The sGFP-AviTag sequence was amplified with primers 5′-CTGACTTCCGGAATGGTGAGCAAGGGCGAGG -3′ (BspE I, forward) and 5′-ACTGAAGATCTTTATTCGTGCCATTCGATTTTC -3′ (Bgl II, reverse), the product linearized with BspEI and BglII, and ligated into the XmaI/BamHI sites of the pIVEX2.3d vector (Roche). .. This approach maintains the pIVEX2.3d multiple cloning site in an almost intact state, which enhances its suitability for production of in-frame C-terminus GFP-tagged proteins.

Polymerase Chain Reaction:

Article Title: A far-red fluorescent protein evolved from a cyanobacterial phycobiliprotein
Article Snippet: .. For creation of smURFP fusions, smURFP was PCR amplified using Phusion High-Fidelity DNA Polymerase (NEB) with primers containing 5’ AgeI and 3’ NotI restriction enzyme sites for N-terminal fusions or with primers containing 5’ AgeI and 3’ BspEI restriction enzyme sites for C-terminal fusions. mGeos2-VEL-ManII-N-10 (Addgene 57551) and Dronpa-PDHA1-N-10 (Addgene 57292) vectors were digested with AgeI and NotI (NEB) and mCherry- αTubulin-C-18 (Addgene 55148) and tdTomato-LaminB1-10 (Addgene 58107) were digested with AgeI and BspEI, dephosphorylated (SAP, Roche), gel purified (Zymoclean Gel DNA Recovery Kit), and ligated (T4 DNA Ligase, Life Technologies) with similarly digested, purified smURFP PCR fragment. .. HEK293A, HT1080 (ATCC), and PC3 (ATCC) cells were grown in Dulbecco’s Modified Eagle’s medium (DMEM, Corning) supplemented with 10% FBS (Atlanta Biologicals) + 1× Penicillin-Streptomycin (Fisher Scientific), which is referred to as growth media, on poly-D-lysine coated glass bottom culture dishes (MatTek, #P35G-0-14-C).

Amplification:

Article Title: Optimizing microarray-based in situ transcription-translation of proteins for MALDI mass spectrometry
Article Snippet: .. The sGFP-AviTag sequence was amplified with primers 5′-CTGACTTCCGGAATGGTGAGCAAGGGCGAGG -3′ (BspE I, forward) and 5′-ACTGAAGATCTTTATTCGTGCCATTCGATTTTC -3′ (Bgl II, reverse), the product linearized with BspEI and BglII, and ligated into the XmaI/BamHI sites of the pIVEX2.3d vector (Roche). .. This approach maintains the pIVEX2.3d multiple cloning site in an almost intact state, which enhances its suitability for production of in-frame C-terminus GFP-tagged proteins.

Article Title: A far-red fluorescent protein evolved from a cyanobacterial phycobiliprotein
Article Snippet: .. For creation of smURFP fusions, smURFP was PCR amplified using Phusion High-Fidelity DNA Polymerase (NEB) with primers containing 5’ AgeI and 3’ NotI restriction enzyme sites for N-terminal fusions or with primers containing 5’ AgeI and 3’ BspEI restriction enzyme sites for C-terminal fusions. mGeos2-VEL-ManII-N-10 (Addgene 57551) and Dronpa-PDHA1-N-10 (Addgene 57292) vectors were digested with AgeI and NotI (NEB) and mCherry- αTubulin-C-18 (Addgene 55148) and tdTomato-LaminB1-10 (Addgene 58107) were digested with AgeI and BspEI, dephosphorylated (SAP, Roche), gel purified (Zymoclean Gel DNA Recovery Kit), and ligated (T4 DNA Ligase, Life Technologies) with similarly digested, purified smURFP PCR fragment. .. HEK293A, HT1080 (ATCC), and PC3 (ATCC) cells were grown in Dulbecco’s Modified Eagle’s medium (DMEM, Corning) supplemented with 10% FBS (Atlanta Biologicals) + 1× Penicillin-Streptomycin (Fisher Scientific), which is referred to as growth media, on poly-D-lysine coated glass bottom culture dishes (MatTek, #P35G-0-14-C).

Plasmid Preparation:

Article Title: Optimizing microarray-based in situ transcription-translation of proteins for MALDI mass spectrometry
Article Snippet: .. The sGFP-AviTag sequence was amplified with primers 5′-CTGACTTCCGGAATGGTGAGCAAGGGCGAGG -3′ (BspE I, forward) and 5′-ACTGAAGATCTTTATTCGTGCCATTCGATTTTC -3′ (Bgl II, reverse), the product linearized with BspEI and BglII, and ligated into the XmaI/BamHI sites of the pIVEX2.3d vector (Roche). .. This approach maintains the pIVEX2.3d multiple cloning site in an almost intact state, which enhances its suitability for production of in-frame C-terminus GFP-tagged proteins.

Purification:

Article Title: A far-red fluorescent protein evolved from a cyanobacterial phycobiliprotein
Article Snippet: .. For creation of smURFP fusions, smURFP was PCR amplified using Phusion High-Fidelity DNA Polymerase (NEB) with primers containing 5’ AgeI and 3’ NotI restriction enzyme sites for N-terminal fusions or with primers containing 5’ AgeI and 3’ BspEI restriction enzyme sites for C-terminal fusions. mGeos2-VEL-ManII-N-10 (Addgene 57551) and Dronpa-PDHA1-N-10 (Addgene 57292) vectors were digested with AgeI and NotI (NEB) and mCherry- αTubulin-C-18 (Addgene 55148) and tdTomato-LaminB1-10 (Addgene 58107) were digested with AgeI and BspEI, dephosphorylated (SAP, Roche), gel purified (Zymoclean Gel DNA Recovery Kit), and ligated (T4 DNA Ligase, Life Technologies) with similarly digested, purified smURFP PCR fragment. .. HEK293A, HT1080 (ATCC), and PC3 (ATCC) cells were grown in Dulbecco’s Modified Eagle’s medium (DMEM, Corning) supplemented with 10% FBS (Atlanta Biologicals) + 1× Penicillin-Streptomycin (Fisher Scientific), which is referred to as growth media, on poly-D-lysine coated glass bottom culture dishes (MatTek, #P35G-0-14-C).

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • bspei  (Roche)
    90
    Roche bspei
    Bspei, supplied by Roche, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bspei/product/Roche
    Average 90 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    bspei - by Bioz Stars, 2020-07
    90/100 stars
      Buy from Supplier

    Image Search Results