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TaKaRa bspei kpni digested pegfp c3 vector
Bspei Kpni Digested Pegfp C3 Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/bspei kpni digested pegfp c3 vector/product/TaKaRa
Average 88 stars, based on 1 article reviews
Price from $9.99 to $1999.99
bspei kpni digested pegfp c3 vector - by Bioz Stars, 2020-07
88/100 stars

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Polymerase Chain Reaction:

Article Title: ABHD17 proteins are novel protein depalmitoylases that regulate N-Ras palmitate turnover and subcellular localization
Article Snippet: .. EGFP-ITM2B was cloned by polymerase chain reaction (PCR) amplification of the ITM2B ORF (open reading frame) from MGC Fully Sequenced Human BRI3 cDNA, clone ID 3163436 (OpenBiosystems; Mississauga, ON), using the forward primer 5’-ATTTAACCCGGGATGGTGAAGATTAGCTTCCAGCC-3’ and the reverse primer 5’-ATTTAAGGTACCTCACACCACCCCGCAGAT-3’, followed by restriction digest and ligation with BspEI/KpnI-digested pEGFP-C3 vector from Clontech (Mountain View, CA). .. EGFP-N-Ras-C181S was generated by Quikchange mutagenesis (Stratagene; La Jolla, CA) using the forward primer 5’-CAACAGCAGTGATGATGGTACCCAGGGTAGTATGGGATTGCCATGTGTGG-3’ and the reverse primer 5’-CCACACATGGCAATCCCATACTACCCTGGG TACCATCATCACTGCTGTTG-3’ with EGFP-N-Ras as the template.

Clone Assay:

Article Title: ABHD17 proteins are novel protein depalmitoylases that regulate N-Ras palmitate turnover and subcellular localization
Article Snippet: .. EGFP-ITM2B was cloned by polymerase chain reaction (PCR) amplification of the ITM2B ORF (open reading frame) from MGC Fully Sequenced Human BRI3 cDNA, clone ID 3163436 (OpenBiosystems; Mississauga, ON), using the forward primer 5’-ATTTAACCCGGGATGGTGAAGATTAGCTTCCAGCC-3’ and the reverse primer 5’-ATTTAAGGTACCTCACACCACCCCGCAGAT-3’, followed by restriction digest and ligation with BspEI/KpnI-digested pEGFP-C3 vector from Clontech (Mountain View, CA). .. EGFP-N-Ras-C181S was generated by Quikchange mutagenesis (Stratagene; La Jolla, CA) using the forward primer 5’-CAACAGCAGTGATGATGGTACCCAGGGTAGTATGGGATTGCCATGTGTGG-3’ and the reverse primer 5’-CCACACATGGCAATCCCATACTACCCTGGG TACCATCATCACTGCTGTTG-3’ with EGFP-N-Ras as the template.

Amplification:

Article Title: ABHD17 proteins are novel protein depalmitoylases that regulate N-Ras palmitate turnover and subcellular localization
Article Snippet: .. EGFP-ITM2B was cloned by polymerase chain reaction (PCR) amplification of the ITM2B ORF (open reading frame) from MGC Fully Sequenced Human BRI3 cDNA, clone ID 3163436 (OpenBiosystems; Mississauga, ON), using the forward primer 5’-ATTTAACCCGGGATGGTGAAGATTAGCTTCCAGCC-3’ and the reverse primer 5’-ATTTAAGGTACCTCACACCACCCCGCAGAT-3’, followed by restriction digest and ligation with BspEI/KpnI-digested pEGFP-C3 vector from Clontech (Mountain View, CA). .. EGFP-N-Ras-C181S was generated by Quikchange mutagenesis (Stratagene; La Jolla, CA) using the forward primer 5’-CAACAGCAGTGATGATGGTACCCAGGGTAGTATGGGATTGCCATGTGTGG-3’ and the reverse primer 5’-CCACACATGGCAATCCCATACTACCCTGGG TACCATCATCACTGCTGTTG-3’ with EGFP-N-Ras as the template.

Plasmid Preparation:

Article Title: ABHD17 proteins are novel protein depalmitoylases that regulate N-Ras palmitate turnover and subcellular localization
Article Snippet: .. EGFP-ITM2B was cloned by polymerase chain reaction (PCR) amplification of the ITM2B ORF (open reading frame) from MGC Fully Sequenced Human BRI3 cDNA, clone ID 3163436 (OpenBiosystems; Mississauga, ON), using the forward primer 5’-ATTTAACCCGGGATGGTGAAGATTAGCTTCCAGCC-3’ and the reverse primer 5’-ATTTAAGGTACCTCACACCACCCCGCAGAT-3’, followed by restriction digest and ligation with BspEI/KpnI-digested pEGFP-C3 vector from Clontech (Mountain View, CA). .. EGFP-N-Ras-C181S was generated by Quikchange mutagenesis (Stratagene; La Jolla, CA) using the forward primer 5’-CAACAGCAGTGATGATGGTACCCAGGGTAGTATGGGATTGCCATGTGTGG-3’ and the reverse primer 5’-CCACACATGGCAATCCCATACTACCCTGGG TACCATCATCACTGCTGTTG-3’ with EGFP-N-Ras as the template.

Ligation:

Article Title: ABHD17 proteins are novel protein depalmitoylases that regulate N-Ras palmitate turnover and subcellular localization
Article Snippet: .. EGFP-ITM2B was cloned by polymerase chain reaction (PCR) amplification of the ITM2B ORF (open reading frame) from MGC Fully Sequenced Human BRI3 cDNA, clone ID 3163436 (OpenBiosystems; Mississauga, ON), using the forward primer 5’-ATTTAACCCGGGATGGTGAAGATTAGCTTCCAGCC-3’ and the reverse primer 5’-ATTTAAGGTACCTCACACCACCCCGCAGAT-3’, followed by restriction digest and ligation with BspEI/KpnI-digested pEGFP-C3 vector from Clontech (Mountain View, CA). .. EGFP-N-Ras-C181S was generated by Quikchange mutagenesis (Stratagene; La Jolla, CA) using the forward primer 5’-CAACAGCAGTGATGATGGTACCCAGGGTAGTATGGGATTGCCATGTGTGG-3’ and the reverse primer 5’-CCACACATGGCAATCCCATACTACCCTGGG TACCATCATCACTGCTGTTG-3’ with EGFP-N-Ras as the template.

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    TaKaRa bspei kpni digested pegfp c3 vector
    Bspei Kpni Digested Pegfp C3 Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bspei kpni digested pegfp c3 vector/product/TaKaRa
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bspei kpni digested pegfp c3 vector - by Bioz Stars, 2020-07
    88/100 stars
      Buy from Supplier

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