rna  (Roche)


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    Structured Review

    Roche rna
    Increase in replicon <t>RNA</t> translation in nocodazole-treated HCV replicon cells. Huh-N1b cells was pre-treated with 20 µM nocodazole for 4 hours and then labeled with <t>BrUTP</t> or with 35 S-Methionine. (A) The BrU-labeled RNA remained colocalized with calnexin (an ER amrker) even after 180 min. (B–D) Proteins were immunoprecipitated with (B) HCV patient serum, (C) Goat anti-NS3 antibody, or (D) Rabbit anti-NPT antibody. The immunoprecipitated products were detected by autoradiography. The nocodazole-pretreated Huh-N1b cells showed about 50% increase in NS3 and NPT translated from replicon RNA, whereas NPT translation in the Huh-Neo control cells was not affected by the nocodazole treatment.
    Rna, supplied by Roche, used in various techniques. Bioz Stars score: 93/100, based on 8252 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rna/product/Roche
    Average 93 stars, based on 8252 article reviews
    Price from $9.99 to $1999.99
    rna - by Bioz Stars, 2020-09
    93/100 stars

    Images

    1) Product Images from "Hepatitis C Virus Translation Preferentially Depends on Active RNA Replication"

    Article Title: Hepatitis C Virus Translation Preferentially Depends on Active RNA Replication

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0043600

    Increase in replicon RNA translation in nocodazole-treated HCV replicon cells. Huh-N1b cells was pre-treated with 20 µM nocodazole for 4 hours and then labeled with BrUTP or with 35 S-Methionine. (A) The BrU-labeled RNA remained colocalized with calnexin (an ER amrker) even after 180 min. (B–D) Proteins were immunoprecipitated with (B) HCV patient serum, (C) Goat anti-NS3 antibody, or (D) Rabbit anti-NPT antibody. The immunoprecipitated products were detected by autoradiography. The nocodazole-pretreated Huh-N1b cells showed about 50% increase in NS3 and NPT translated from replicon RNA, whereas NPT translation in the Huh-Neo control cells was not affected by the nocodazole treatment.
    Figure Legend Snippet: Increase in replicon RNA translation in nocodazole-treated HCV replicon cells. Huh-N1b cells was pre-treated with 20 µM nocodazole for 4 hours and then labeled with BrUTP or with 35 S-Methionine. (A) The BrU-labeled RNA remained colocalized with calnexin (an ER amrker) even after 180 min. (B–D) Proteins were immunoprecipitated with (B) HCV patient serum, (C) Goat anti-NS3 antibody, or (D) Rabbit anti-NPT antibody. The immunoprecipitated products were detected by autoradiography. The nocodazole-pretreated Huh-N1b cells showed about 50% increase in NS3 and NPT translated from replicon RNA, whereas NPT translation in the Huh-Neo control cells was not affected by the nocodazole treatment.

    Techniques Used: Labeling, Immunoprecipitation, Autoradiography

    The translocation of newly-synthesized HCV RNA. HCV replicon cells were labeled with BrUTP (A) or 3 H-Uridine (B) in the presence of actinomycin D and chased for up to 180 minutes. (A) Immunofluorescence staining with anti-BrdU and other organelle antibodies shows the colocalization of BrU-labeled HCV RNA with ER initially (30 min) and then with Golgi (180 min). (B) Fractionation of ER and Golgi by sucrose gradient. Fraction numbers and their gradient positions are noted at the bottom. 3 H-Uridine-labeled RNA in the ER (fraction 4) and the Golgi (fraction 6–8) fractions were collected, and the radioactivity of 3 H-Uridine-labeled RNA was counted. Immunoblotting of ER and Golgi makers demonstrates the separation of ER and Golgi by sucrose gradient fractionation.
    Figure Legend Snippet: The translocation of newly-synthesized HCV RNA. HCV replicon cells were labeled with BrUTP (A) or 3 H-Uridine (B) in the presence of actinomycin D and chased for up to 180 minutes. (A) Immunofluorescence staining with anti-BrdU and other organelle antibodies shows the colocalization of BrU-labeled HCV RNA with ER initially (30 min) and then with Golgi (180 min). (B) Fractionation of ER and Golgi by sucrose gradient. Fraction numbers and their gradient positions are noted at the bottom. 3 H-Uridine-labeled RNA in the ER (fraction 4) and the Golgi (fraction 6–8) fractions were collected, and the radioactivity of 3 H-Uridine-labeled RNA was counted. Immunoblotting of ER and Golgi makers demonstrates the separation of ER and Golgi by sucrose gradient fractionation.

    Techniques Used: Translocation Assay, Synthesized, Labeling, Immunofluorescence, Staining, Fractionation, Radioactivity

    2) Product Images from "Nonstructural Protein 5A Is Incorporated into Hepatitis C Virus Low-Density Particle through Interaction with Core Protein and Microtubules during Intracellular Transport"

    Article Title: Nonstructural Protein 5A Is Incorporated into Hepatitis C Virus Low-Density Particle through Interaction with Core Protein and Microtubules during Intracellular Transport

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0099022

    Nocodazole affects the movement of viral replication complexes (RCs) to the LDs. (A) The HCV-infected cells (at day 10 p.i.) were transfected with BrUTP in the presence of actinomycin D for 1 h and then treated with either 10 µM nocodazole or 10 µM taxol for 1 h. The cells were co-stained with mouse MAb against bromodeoxyuridine (green) and rabbit polyclonal antibodies against Core (RR8) (red). LDs and nuclei were stained with BODYPI 493/503 (blue) and DAPI (cyan), respectively. Enlarged views of parts of every image are shown (insets). (B) The number of BrUTP-labeled viral RNA was counted manually using an original magnification of ×630 and followed by a quantitation analysis performed by an observer blinded to the experimental treatment. (C) The average distance between the center of the signal emitted by the BrUTP-labeled viral RNA and the nearest edge of LD (HCV RNA-LD distance) were analyzed by using Zeiss LSM Zen software. A total of 20 cells were used for quantitation and calculation of the HCV RNA-LD distance and the number of BrUTP-labeled viral RNA from two independent experiments and error bars represent standard deviations of the mean. N.S., non-significance; *, P
    Figure Legend Snippet: Nocodazole affects the movement of viral replication complexes (RCs) to the LDs. (A) The HCV-infected cells (at day 10 p.i.) were transfected with BrUTP in the presence of actinomycin D for 1 h and then treated with either 10 µM nocodazole or 10 µM taxol for 1 h. The cells were co-stained with mouse MAb against bromodeoxyuridine (green) and rabbit polyclonal antibodies against Core (RR8) (red). LDs and nuclei were stained with BODYPI 493/503 (blue) and DAPI (cyan), respectively. Enlarged views of parts of every image are shown (insets). (B) The number of BrUTP-labeled viral RNA was counted manually using an original magnification of ×630 and followed by a quantitation analysis performed by an observer blinded to the experimental treatment. (C) The average distance between the center of the signal emitted by the BrUTP-labeled viral RNA and the nearest edge of LD (HCV RNA-LD distance) were analyzed by using Zeiss LSM Zen software. A total of 20 cells were used for quantitation and calculation of the HCV RNA-LD distance and the number of BrUTP-labeled viral RNA from two independent experiments and error bars represent standard deviations of the mean. N.S., non-significance; *, P

    Techniques Used: Infection, Transfection, Staining, Labeling, Quantitation Assay, Software

    3) Product Images from "Nonstructural Protein 5A Is Incorporated into Hepatitis C Virus Low-Density Particle through Interaction with Core Protein and Microtubules during Intracellular Transport"

    Article Title: Nonstructural Protein 5A Is Incorporated into Hepatitis C Virus Low-Density Particle through Interaction with Core Protein and Microtubules during Intracellular Transport

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0099022

    Nocodazole affects the movement of viral replication complexes (RCs) to the LDs. (A) The HCV-infected cells (at day 10 p.i.) were transfected with BrUTP in the presence of actinomycin D for 1 h and then treated with either 10 µM nocodazole or 10 µM taxol for 1 h. The cells were co-stained with mouse MAb against bromodeoxyuridine (green) and rabbit polyclonal antibodies against Core (RR8) (red). LDs and nuclei were stained with BODYPI 493/503 (blue) and DAPI (cyan), respectively. Enlarged views of parts of every image are shown (insets). (B) The number of BrUTP-labeled viral RNA was counted manually using an original magnification of ×630 and followed by a quantitation analysis performed by an observer blinded to the experimental treatment. (C) The average distance between the center of the signal emitted by the BrUTP-labeled viral RNA and the nearest edge of LD (HCV RNA-LD distance) were analyzed by using Zeiss LSM Zen software. A total of 20 cells were used for quantitation and calculation of the HCV RNA-LD distance and the number of BrUTP-labeled viral RNA from two independent experiments and error bars represent standard deviations of the mean. N.S., non-significance; *, P
    Figure Legend Snippet: Nocodazole affects the movement of viral replication complexes (RCs) to the LDs. (A) The HCV-infected cells (at day 10 p.i.) were transfected with BrUTP in the presence of actinomycin D for 1 h and then treated with either 10 µM nocodazole or 10 µM taxol for 1 h. The cells were co-stained with mouse MAb against bromodeoxyuridine (green) and rabbit polyclonal antibodies against Core (RR8) (red). LDs and nuclei were stained with BODYPI 493/503 (blue) and DAPI (cyan), respectively. Enlarged views of parts of every image are shown (insets). (B) The number of BrUTP-labeled viral RNA was counted manually using an original magnification of ×630 and followed by a quantitation analysis performed by an observer blinded to the experimental treatment. (C) The average distance between the center of the signal emitted by the BrUTP-labeled viral RNA and the nearest edge of LD (HCV RNA-LD distance) were analyzed by using Zeiss LSM Zen software. A total of 20 cells were used for quantitation and calculation of the HCV RNA-LD distance and the number of BrUTP-labeled viral RNA from two independent experiments and error bars represent standard deviations of the mean. N.S., non-significance; *, P

    Techniques Used: Infection, Transfection, Staining, Labeling, Quantitation Assay, Software

    Related Articles

    Transfection:

    Article Title: Hepatitis B Viral DNA Decline at Loss of HBeAg Is Mainly Explained by Reduced cccDNA Load - Down-Regulated Transcription of PgRNA Has Limited Impact
    Article Snippet: .. Extraction of DNA and RNA from Cells DNA and RNA were extracted from transfected and non-transfected hepatoma cells in a Magnapure robot (Roche Applied Science, Germany) using the Total NA protocol. .. Harvested cells were washed in 1 mL PBS and after centrifugation at 5000 rpm for 3 min the pellet was re-suspended in 800 µL RLT lysis buffer (Qiagen Sciences, MD, USA) before extraction.

    Synthesized:

    Article Title: Chronic hypoxia‐induced slug promotes invasive behavior of prostate cancer cells by activating expression of ephrin‐B1, et al. Chronic hypoxia‐induced slug promotes invasive behavior of prostate cancer cells by activating expression of ephrin‐B1
    Article Snippet: .. 2.5 Real‐time quantitative RT‐PCR First‐strand cDNA was synthesized from the total RNA using ThermoScript RT‐PCR System (Roche, Indianapolis, IN, USA). .. PCR was performed on a LightCycler system (Roche) using LightCycler FastStart DNA Master SYBR Green I reaction mix (Roche) and QuantiTect Primer Assays (QIAGEN, Hilden, Germany).

    Isolation:

    Article Title: MERS-coronavirus replication induces severe in vitro cytopathology and is strongly inhibited by cyclosporin A or interferon-? treatment
    Article Snippet: .. RNA from 200 µl culture medium of CoV-infected cells was isolated with a MagnaPure LC Total Nucleic Acid Isolation kit (Roche) and eluted in 100 µl. .. RT-PCR conditions for quantifying MERS-CoV and SARS-CoV RNA and amplification parameters have been described previously ( ; ).

    Article Title: Homologous Recombination Occurs in Entamoeba and Is Enhanced during Growth Stress and Stage Conversion
    Article Snippet: .. RNA purification and Real Time RT-PCR Entamoeba cells were harvested and suspended immediately in TriZol reagent (Invitrogen) and RNA was isolated and treated with DNase I (Roche) according to manufacturer's protocol. .. RNA was reverse transcribed into cDNA using Superscript III reverse transcriptase (Invitrogen) and random hexamers used as a primer.

    Article Title: Genome-wide analyses reveal the IRE1a-XBP1 pathway promotes T helper cell differentiation by resolving secretory stress and accelerating proliferation
    Article Snippet: .. Reverse transcription quantitative PCR (RT-qPCR) Total RNA was isolated from two million cells by SV total RNA isolation kit (Promega). cDNA was prepared by annealing 500 ng RNA with oligo dT as per the manufacturer’s instructions (Transcriptor High Fidelity cDNA Synthesis kit, Roche). ..

    Quantitative RT-PCR:

    Article Title: Chronic hypoxia‐induced slug promotes invasive behavior of prostate cancer cells by activating expression of ephrin‐B1, et al. Chronic hypoxia‐induced slug promotes invasive behavior of prostate cancer cells by activating expression of ephrin‐B1
    Article Snippet: .. 2.5 Real‐time quantitative RT‐PCR First‐strand cDNA was synthesized from the total RNA using ThermoScript RT‐PCR System (Roche, Indianapolis, IN, USA). .. PCR was performed on a LightCycler system (Roche) using LightCycler FastStart DNA Master SYBR Green I reaction mix (Roche) and QuantiTect Primer Assays (QIAGEN, Hilden, Germany).

    Article Title: Homologous Recombination Occurs in Entamoeba and Is Enhanced during Growth Stress and Stage Conversion
    Article Snippet: .. RNA purification and Real Time RT-PCR Entamoeba cells were harvested and suspended immediately in TriZol reagent (Invitrogen) and RNA was isolated and treated with DNase I (Roche) according to manufacturer's protocol. .. RNA was reverse transcribed into cDNA using Superscript III reverse transcriptase (Invitrogen) and random hexamers used as a primer.

    Article Title: Genome-wide analyses reveal the IRE1a-XBP1 pathway promotes T helper cell differentiation by resolving secretory stress and accelerating proliferation
    Article Snippet: .. Reverse transcription quantitative PCR (RT-qPCR) Total RNA was isolated from two million cells by SV total RNA isolation kit (Promega). cDNA was prepared by annealing 500 ng RNA with oligo dT as per the manufacturer’s instructions (Transcriptor High Fidelity cDNA Synthesis kit, Roche). ..

    Purification:

    Article Title: Homologous Recombination Occurs in Entamoeba and Is Enhanced during Growth Stress and Stage Conversion
    Article Snippet: .. RNA purification and Real Time RT-PCR Entamoeba cells were harvested and suspended immediately in TriZol reagent (Invitrogen) and RNA was isolated and treated with DNase I (Roche) according to manufacturer's protocol. .. RNA was reverse transcribed into cDNA using Superscript III reverse transcriptase (Invitrogen) and random hexamers used as a primer.

    Real-time Polymerase Chain Reaction:

    Article Title: Genome-wide analyses reveal the IRE1a-XBP1 pathway promotes T helper cell differentiation by resolving secretory stress and accelerating proliferation
    Article Snippet: .. Reverse transcription quantitative PCR (RT-qPCR) Total RNA was isolated from two million cells by SV total RNA isolation kit (Promega). cDNA was prepared by annealing 500 ng RNA with oligo dT as per the manufacturer’s instructions (Transcriptor High Fidelity cDNA Synthesis kit, Roche). ..

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Chronic hypoxia‐induced slug promotes invasive behavior of prostate cancer cells by activating expression of ephrin‐B1, et al. Chronic hypoxia‐induced slug promotes invasive behavior of prostate cancer cells by activating expression of ephrin‐B1
    Article Snippet: .. 2.5 Real‐time quantitative RT‐PCR First‐strand cDNA was synthesized from the total RNA using ThermoScript RT‐PCR System (Roche, Indianapolis, IN, USA). .. PCR was performed on a LightCycler system (Roche) using LightCycler FastStart DNA Master SYBR Green I reaction mix (Roche) and QuantiTect Primer Assays (QIAGEN, Hilden, Germany).

    Article Title: Arabidopsis ETO1 specifically interacts with and negatively regulates type 2 1-aminocyclopropane-1-carboxylate synthases
    Article Snippet: .. RNA (1 μg) was used in each RT-PCR following the manufacturer's protocol (Titan One Tube RT-PCR System, Roche Diagnostics). ..

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    Roche brutp fugene 6
    Brutp Fugene 6, supplied by Roche, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/brutp fugene 6/product/Roche
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    brutp fugene 6 - by Bioz Stars, 2020-09
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