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Millipore broth
Broth, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/broth/product/Millipore
Average 93 stars, based on 2 article reviews
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broth - by Bioz Stars, 2020-04
93/100 stars

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Clone Assay:

Article Title: Brucella melitensis VjbR and C12-HSL regulons: contributions of the N-dodecanoyl homoserine lactone signaling molecule and LuxR homologue VjbR to gene expression
Article Snippet: Bacteria, macrophage strains and growth conditions Escherichia coli DH5α™-T1R competent cells were used for cloning and routinely grown on Luria-Bertani (LB, Difco Laboratories) overnight at 37°C with supplemental kanamycin (100 mg/l) or carbenicillin (100 mg/l) as needed. .. B. melitensis 16M was grown on tryptic soy agar or broth (TSA or TSB) and J774A.1 murine macrophage-like cells were maintained in T-75 flasks in Dulbecco's modified Eagle's medium, HEPES modification (DMEM), supplemented with 1× MEM non-essential amino acids (Sigma, St Louis, MO), 0.37% sodium bicarbonate and 10% fetal bovine serum at 37°C with 5% CO2 .

Centrifugation:

Article Title: Characterization of the AggR Regulon in Enteroaggregative Escherichia coli
Article Snippet: .. One milliliter of bacterial broth (OD600 , 0.8) was pelleted by centrifugation, washed in 1 ml of phosphate-buffered saline (PBS), and incubated overnight at 4°C in 2% paraformaldehyde (Sigma-Aldrich, St. Louis, MO), 2.5% glutaraldehyde, and 0.1 M CaCo buffer. ..

Cytometry:

Article Title: Septicaemia models using Streptococcus pneumoniae and Listeria monocytogenes: understanding the role of complement properdin
Article Snippet: Flow cytometric analyses To quantify the phagocytic uptake of S. pneumoniae D39 by splenic macrophages, extracellular fluorescence was quenched with 0.2 (w/v) % trypanblue prior to flow cytometry. .. To prepare the labelled bacteria, S. pneumoniae D39 were grown in broth (stationary, 37 °C), and 109 bacteria/ml were resuspended in 0.1 mg/ml FITC (Sigma-Aldrich, 0.1 M NaHCO3 pH 9.0).

Quantitative RT-PCR:

Article Title: Ethanolamine is a Valuable Nutrient Source that Impacts Clostridium difficile Pathogenesis
Article Snippet: Paragraph title: RNA isolation and quantitative reverse transcription PCR analysis (qRT-PCR) ... Cultures were then diluted 1:10 into 70:30 broth (70% SMC, 30% BHIS medium) , with and without the addition of 15 mM ethanolamine hydrochloride (Sigma-Aldrich).

Incubation:

Article Title: Antibacterial and Antifungal Activities of Poloxamer Micelles Containing Ceragenin CSA-131 on Ciliated Tissues
Article Snippet: CSA-131 (100 µg/mL), in TSB or SDB and in the presence or absence of pluronic (4% or 5%), was added to the wells at concentrations ranging from 1 to 256 µg/mL, and each plate was incubated at 37 °C for 24 h. Wells, including well edges, were scraped thoroughly with a plastic spatula. .. Well contents were removed in 1 mL of neutralizing broth (Dey-Engley, Sigma–Aldrich) and placed in a sonicating water bath (Fisher Scientific FS60, 42 kHz, 100 W, Pittsburg, PA, USA) to disrupt biofilms.

Article Title: Mycobacterium avium Infections of Acanthamoeba Strains: Host Strain Variability, Grazing-Acquired Infections, and Altered Dynamics of Inactivation with Monochloramine ▿ Strains: Host Strain Variability, Grazing-Acquired Infections, and Altered Dynamics of Inactivation with Monochloramine ▿ †
Article Snippet: Mycobacterium avium subsp. hominissuis 104 ( ) was cultured on Middlebrook 7H9/OADC (oleic acid-albumin-dextrose-catalase) broth (Sigma-Aldrich). .. Cocultures were incubated at 20°C in the dark and were washed and treated weekly with amikacin to minimize the potential for extra-amoebal growth of M. avium , which was confirmed by daily monitoring by phase-contrast microscopy.

Article Title: Evaluation of genetic and phenotypic consistency of Bacillus coagulans MTCC 5856: a commercial probiotic strain
Article Snippet: After incubation, the broth was filtered through 0.22 micron (Sartorius, India) and analyzed for lactic acid content by using Megazyme kit (K-DLATE 10/04) as per instructions (Megazyme International Ireland, IDA Business Park, Wicklow, Ireland). .. Above filtered broth (50 mL) of the overnight grown B. coagulans MTCC 5856 was added to 25 mL Aliquat 336 (50 % w/v in Oleyl alcohol) (Sigma Chemical Co., St Louis, MO, USA) and then stirred for 45 min.

Article Title: Characterization of the AggR Regulon in Enteroaggregative Escherichia coli
Article Snippet: .. One milliliter of bacterial broth (OD600 , 0.8) was pelleted by centrifugation, washed in 1 ml of phosphate-buffered saline (PBS), and incubated overnight at 4°C in 2% paraformaldehyde (Sigma-Aldrich, St. Louis, MO), 2.5% glutaraldehyde, and 0.1 M CaCo buffer. ..

Article Title: Septicaemia models using Streptococcus pneumoniae and Listeria monocytogenes: understanding the role of complement properdin
Article Snippet: To prepare the labelled bacteria, S. pneumoniae D39 were grown in broth (stationary, 37 °C), and 109 bacteria/ml were resuspended in 0.1 mg/ml FITC (Sigma-Aldrich, 0.1 M NaHCO3 pH 9.0). .. Splenic cell suspensions were prepared from a pool each of two properdin-deficient and wildtype mice, and adherent cells were incubated in the presence of genotype-matched serum with labelled S. pneumoniae for 1 h prior to analysis.

Activity Assay:

Article Title: 3-Substituted N-Benzylpyrazine-2-carboxamide Derivatives: Synthesis, Antimycobacterial and Antibacterial Evaluation
Article Snippet: Paragraph title: 3.4.3. Antimycobacterial In Vitro Activity Screening Against Mycobacterium Smegmatis ... The culturing medium was Middlebrook 7H9 (MB) broth (Sigma-Aldrich), enriched with 0.4% of glycerol (Sigma-Aldrich) and 10% of Middlebrook OADC growth supplement (Himedia, Mumbai, India).

Article Title: Structural Formation and Photocatalytic Activity of Magnetron Sputtered Titania and Doped-Titania Coatings
Article Snippet: Measurements of the antimicrobial activity of selected coatings deposited onto 304 2B stainless steel substrates were performed using ISO 27447:2009 as guidance (with minor modifications) [ ]. .. At selected time points (0, 12, 24 and 48 h), surfaces were removed and vortexed for 1 min in neutralizing broth (20 g·L−1 Soya Lectin (Holland and Barrett, Nuneaton, UK) and 30 g·L−1 Tween 80 (Sigma Aldrich, Gillingham, UK) to remove any surviving bacteria.

Cell Culture:

Article Title: Pathways of Pathogenicity: Transcriptional Stages of Germination in the Fatal Fungal Pathogen Rhizopus delemar
Article Snippet: .. R. delemar was cultured with Sabouraud dextrose agar or broth (10 g/liter mycological peptone, 20 g/liter dextrose), sourced from Sigma-Aldrich, at room temperature. ..

Article Title: Antibacterial Properties of Visible-Light-Responsive Carbon-Containing Titanium Dioxide Photocatalytic Nanoparticles against Anthrax
Article Snippet: .. B. cereus (ATCC 13061) and B. thuringiensis (ATCC 35646) were maintained and cultured in BAPs or BHIB at 30 °C, and B. subtilis (ATCC 39090) was maintained and cultured in trypticase soy agar or broth (Sigma-Aldrich, St. Louis, MO, USA) at 37 °C [ , ]. ..

Article Title: Mycobacterium avium Infections of Acanthamoeba Strains: Host Strain Variability, Grazing-Acquired Infections, and Altered Dynamics of Inactivation with Monochloramine ▿ Strains: Host Strain Variability, Grazing-Acquired Infections, and Altered Dynamics of Inactivation with Monochloramine ▿ †
Article Snippet: .. Mycobacterium avium subsp. hominissuis 104 ( ) was cultured on Middlebrook 7H9/OADC (oleic acid-albumin-dextrose-catalase) broth (Sigma-Aldrich). .. M. avium was added to Acanthamoeba monolayers at a multiplicity of infection of 10:1 and treated with amikacin as described previously ( ).

Modification:

Article Title: Mycobacterium avium Infections of Acanthamoeba Strains: Host Strain Variability, Grazing-Acquired Infections, and Altered Dynamics of Inactivation with Monochloramine ▿ Strains: Host Strain Variability, Grazing-Acquired Infections, and Altered Dynamics of Inactivation with Monochloramine ▿ †
Article Snippet: Mycobacterium avium subsp. hominissuis 104 ( ) was cultured on Middlebrook 7H9/OADC (oleic acid-albumin-dextrose-catalase) broth (Sigma-Aldrich). .. Acid-fast staining of cocultures was performed using a modified Ziehl-Neelson staining protocol , and infected amoebae ( > 50 per time point) and intracellular mycobacteria were counted using a 100× objective microscope (Axioplan 2; Carl Zeiss Microimaging GmbH).

Article Title: Brucella melitensis VjbR and C12-HSL regulons: contributions of the N-dodecanoyl homoserine lactone signaling molecule and LuxR homologue VjbR to gene expression
Article Snippet: .. B. melitensis 16M was grown on tryptic soy agar or broth (TSA or TSB) and J774A.1 murine macrophage-like cells were maintained in T-75 flasks in Dulbecco's modified Eagle's medium, HEPES modification (DMEM), supplemented with 1× MEM non-essential amino acids (Sigma, St Louis, MO), 0.37% sodium bicarbonate and 10% fetal bovine serum at 37°C with 5% CO2 . ..

Derivative Assay:

Article Title: Deficiency of the ferrous iron transporter FeoAB is linked with metronidazole resistance in Bacteroides fragilis
Article Snippet: All feoAB constructs were derived from BF638R (Table ). .. Bacterial strains were grown at 37°C as described previously using brain heart infusion (BHI) media supplemented with 15 mg/L haemin (BHIS) for B. fragilis isolates in H. M. Wexler's laboratory (Anaerobe Systems, Morgan Hill, CA, USA) or BHI supplemented with 1 g/L l -cysteine, 5 mg/L haemin, 1 mg/L resazurin and 20 mL/L 10% NaHCO3 (BHIS-ER) in E. R. Rocha's laboratory and Luria–Bertani agar or broth (Sigma) for Escherichia coli DH10B.

Electron Microscopy:

Article Title: Characterization of the AggR Regulon in Enteroaggregative Escherichia coli
Article Snippet: One milliliter of bacterial broth (OD600 , 0.8) was pelleted by centrifugation, washed in 1 ml of phosphate-buffered saline (PBS), and incubated overnight at 4°C in 2% paraformaldehyde (Sigma-Aldrich, St. Louis, MO), 2.5% glutaraldehyde, and 0.1 M CaCo buffer. .. The grids were then negatively stained with 2% uranyl acetate (Electron Microscopy Sciences Inc., Fort Washington, PA) for 5 s. The sample was blotted dry and viewed on a Jeol JEM1230 transmission electron microscope (TEM) (80 kV) at the Advanced Microscopy Laboratory at the University of Virginia (AML-UVA).

Flow Cytometry:

Article Title: Septicaemia models using Streptococcus pneumoniae and Listeria monocytogenes: understanding the role of complement properdin
Article Snippet: Paragraph title: Flow cytometric analyses ... To prepare the labelled bacteria, S. pneumoniae D39 were grown in broth (stationary, 37 °C), and 109 bacteria/ml were resuspended in 0.1 mg/ml FITC (Sigma-Aldrich, 0.1 M NaHCO3 pH 9.0).

Infection:

Article Title: Mycobacterium avium Infections of Acanthamoeba Strains: Host Strain Variability, Grazing-Acquired Infections, and Altered Dynamics of Inactivation with Monochloramine ▿ Strains: Host Strain Variability, Grazing-Acquired Infections, and Altered Dynamics of Inactivation with Monochloramine ▿ †
Article Snippet: Mycobacterium avium subsp. hominissuis 104 ( ) was cultured on Middlebrook 7H9/OADC (oleic acid-albumin-dextrose-catalase) broth (Sigma-Aldrich). .. M. avium was added to Acanthamoeba monolayers at a multiplicity of infection of 10:1 and treated with amikacin as described previously ( ).

Article Title: Septicaemia models using Streptococcus pneumoniae and Listeria monocytogenes: understanding the role of complement properdin
Article Snippet: To prepare the labelled bacteria, S. pneumoniae D39 were grown in broth (stationary, 37 °C), and 109 bacteria/ml were resuspended in 0.1 mg/ml FITC (Sigma-Aldrich, 0.1 M NaHCO3 pH 9.0). .. To characterise the dendritic cell phenotype after infection with heat-killed L. monocytogenes (MOI 0.2), surface expressions of CD11c and CD40, CD80, CD86 or MHCII, respectively (BD Biosciences Pharmingen, Oxford, UK), were analysed.

Generated:

Article Title: FdhTU-Modulated Formate Dehydrogenase Expression and Electron Donor Availability Enhance Recovery of Campylobacter jejuni following Host Cell Infection
Article Snippet: All strains were grown in/on Mueller-Hinton (MH) broth (Oxoid, Ltd.) or agar plates at 38°C in a standard C. jejuni growth atmosphere of 6% O2 and 12% CO2 generated using a Sanyo Tri-Gas incubator (for plate growth) or using the Oxoid CampyGen system (for shaking broth cultures). .. All genetic manipulations were performed in E. coli DH5α cells grown on LB plates or broth (Sigma) supplemented with 100 μg of ampicillin (Sigma)/ml, 25 μg of kanamycin/ml, or 30 μg of chloramphenicol/ml.

Transmission Assay:

Article Title: Characterization of the AggR Regulon in Enteroaggregative Escherichia coli
Article Snippet: One milliliter of bacterial broth (OD600 , 0.8) was pelleted by centrifugation, washed in 1 ml of phosphate-buffered saline (PBS), and incubated overnight at 4°C in 2% paraformaldehyde (Sigma-Aldrich, St. Louis, MO), 2.5% glutaraldehyde, and 0.1 M CaCo buffer. .. The grids were then negatively stained with 2% uranyl acetate (Electron Microscopy Sciences Inc., Fort Washington, PA) for 5 s. The sample was blotted dry and viewed on a Jeol JEM1230 transmission electron microscope (TEM) (80 kV) at the Advanced Microscopy Laboratory at the University of Virginia (AML-UVA).

Polymerase Chain Reaction:

Article Title: Ethanolamine is a Valuable Nutrient Source that Impacts Clostridium difficile Pathogenesis
Article Snippet: Paragraph title: RNA isolation and quantitative reverse transcription PCR analysis (qRT-PCR) ... Cultures were then diluted 1:10 into 70:30 broth (70% SMC, 30% BHIS medium) , with and without the addition of 15 mM ethanolamine hydrochloride (Sigma-Aldrich).

Transmission Electron Microscopy:

Article Title: Characterization of the AggR Regulon in Enteroaggregative Escherichia coli
Article Snippet: Paragraph title: TEM. ... One milliliter of bacterial broth (OD600 , 0.8) was pelleted by centrifugation, washed in 1 ml of phosphate-buffered saline (PBS), and incubated overnight at 4°C in 2% paraformaldehyde (Sigma-Aldrich, St. Louis, MO), 2.5% glutaraldehyde, and 0.1 M CaCo buffer.

Fluorescence:

Article Title: Septicaemia models using Streptococcus pneumoniae and Listeria monocytogenes: understanding the role of complement properdin
Article Snippet: Flow cytometric analyses To quantify the phagocytic uptake of S. pneumoniae D39 by splenic macrophages, extracellular fluorescence was quenched with 0.2 (w/v) % trypanblue prior to flow cytometry. .. To prepare the labelled bacteria, S. pneumoniae D39 were grown in broth (stationary, 37 °C), and 109 bacteria/ml were resuspended in 0.1 mg/ml FITC (Sigma-Aldrich, 0.1 M NaHCO3 pH 9.0).

Isolation:

Article Title: Ethanolamine is a Valuable Nutrient Source that Impacts Clostridium difficile Pathogenesis
Article Snippet: Paragraph title: RNA isolation and quantitative reverse transcription PCR analysis (qRT-PCR) ... Cultures were then diluted 1:10 into 70:30 broth (70% SMC, 30% BHIS medium) , with and without the addition of 15 mM ethanolamine hydrochloride (Sigma-Aldrich).

Article Title: Mycobacterium avium Infections of Acanthamoeba Strains: Host Strain Variability, Grazing-Acquired Infections, and Altered Dynamics of Inactivation with Monochloramine ▿ Strains: Host Strain Variability, Grazing-Acquired Infections, and Altered Dynamics of Inactivation with Monochloramine ▿ †
Article Snippet: Eight Acanthamoeba strains were studied, four of which were recently isolated from the environment (biofilm from a drinking water distribution system, forest soil, and two from marsh sediment) and four “laboratory strains” which had been passaged many times on nutrient-rich medium (see Table S1 in the supplemental material). .. Mycobacterium avium subsp. hominissuis 104 ( ) was cultured on Middlebrook 7H9/OADC (oleic acid-albumin-dextrose-catalase) broth (Sigma-Aldrich).

Microscopy:

Article Title: Mycobacterium avium Infections of Acanthamoeba Strains: Host Strain Variability, Grazing-Acquired Infections, and Altered Dynamics of Inactivation with Monochloramine ▿ Strains: Host Strain Variability, Grazing-Acquired Infections, and Altered Dynamics of Inactivation with Monochloramine ▿ †
Article Snippet: Mycobacterium avium subsp. hominissuis 104 ( ) was cultured on Middlebrook 7H9/OADC (oleic acid-albumin-dextrose-catalase) broth (Sigma-Aldrich). .. Cocultures were incubated at 20°C in the dark and were washed and treated weekly with amikacin to minimize the potential for extra-amoebal growth of M. avium , which was confirmed by daily monitoring by phase-contrast microscopy.

Article Title: Characterization of the AggR Regulon in Enteroaggregative Escherichia coli
Article Snippet: One milliliter of bacterial broth (OD600 , 0.8) was pelleted by centrifugation, washed in 1 ml of phosphate-buffered saline (PBS), and incubated overnight at 4°C in 2% paraformaldehyde (Sigma-Aldrich, St. Louis, MO), 2.5% glutaraldehyde, and 0.1 M CaCo buffer. .. The grids were then negatively stained with 2% uranyl acetate (Electron Microscopy Sciences Inc., Fort Washington, PA) for 5 s. The sample was blotted dry and viewed on a Jeol JEM1230 transmission electron microscope (TEM) (80 kV) at the Advanced Microscopy Laboratory at the University of Virginia (AML-UVA).

Mouse Assay:

Article Title: Septicaemia models using Streptococcus pneumoniae and Listeria monocytogenes: understanding the role of complement properdin
Article Snippet: To prepare the labelled bacteria, S. pneumoniae D39 were grown in broth (stationary, 37 °C), and 109 bacteria/ml were resuspended in 0.1 mg/ml FITC (Sigma-Aldrich, 0.1 M NaHCO3 pH 9.0). .. Splenic cell suspensions were prepared from a pool each of two properdin-deficient and wildtype mice, and adherent cells were incubated in the presence of genotype-matched serum with labelled S. pneumoniae for 1 h prior to analysis.

Sequencing:

Article Title: Mycobacterium avium Infections of Acanthamoeba Strains: Host Strain Variability, Grazing-Acquired Infections, and Altered Dynamics of Inactivation with Monochloramine ▿ Strains: Host Strain Variability, Grazing-Acquired Infections, and Altered Dynamics of Inactivation with Monochloramine ▿ †
Article Snippet: All strains were members of sequence type T4 , with the exception of Acanthamoeba sp. strain F2B (type T13) and Acanthamoeba hatchetii (type 11) (GenBank accession no. ) (see Fig. S1 in the supplemental material). .. Mycobacterium avium subsp. hominissuis 104 ( ) was cultured on Middlebrook 7H9/OADC (oleic acid-albumin-dextrose-catalase) broth (Sigma-Aldrich).

Construct:

Article Title: Deficiency of the ferrous iron transporter FeoAB is linked with metronidazole resistance in Bacteroides fragilis
Article Snippet: All feoAB constructs were derived from BF638R (Table ). .. Bacterial strains were grown at 37°C as described previously using brain heart infusion (BHI) media supplemented with 15 mg/L haemin (BHIS) for B. fragilis isolates in H. M. Wexler's laboratory (Anaerobe Systems, Morgan Hill, CA, USA) or BHI supplemented with 1 g/L l -cysteine, 5 mg/L haemin, 1 mg/L resazurin and 20 mL/L 10% NaHCO3 (BHIS-ER) in E. R. Rocha's laboratory and Luria–Bertani agar or broth (Sigma) for Escherichia coli DH10B.

Functional Assay:

Article Title: Antibacterial Properties of Visible-Light-Responsive Carbon-Containing Titanium Dioxide Photocatalytic Nanoparticles against Anthrax
Article Snippet: B. anthracis (ATCC 14186) containing both pXO1 and pXO2 plasmids to express functional LT and edema toxin (ET) was grown on blood agar plates (BAPs) and maintained in brain–heart infusion broth (BHIB) (Sigma-Aldrich, St. Louis, MO, USA) using previously described methods [ , , , , , , , , , ]. .. B. cereus (ATCC 13061) and B. thuringiensis (ATCC 35646) were maintained and cultured in BAPs or BHIB at 30 °C, and B. subtilis (ATCC 39090) was maintained and cultured in trypticase soy agar or broth (Sigma-Aldrich, St. Louis, MO, USA) at 37 °C [ , ].

In Vitro:

Article Title: 3-Substituted N-Benzylpyrazine-2-carboxamide Derivatives: Synthesis, Antimycobacterial and Antibacterial Evaluation
Article Snippet: Paragraph title: 3.4.3. Antimycobacterial In Vitro Activity Screening Against Mycobacterium Smegmatis ... The culturing medium was Middlebrook 7H9 (MB) broth (Sigma-Aldrich), enriched with 0.4% of glycerol (Sigma-Aldrich) and 10% of Middlebrook OADC growth supplement (Himedia, Mumbai, India).

Produced:

Article Title: Evaluation of genetic and phenotypic consistency of Bacillus coagulans MTCC 5856: a commercial probiotic strain
Article Snippet: The optical rotation of lactic acid produced by B. coagulans MTCC 5856 was confirmed by Polarimetric method (Kahya et al. ). .. Above filtered broth (50 mL) of the overnight grown B. coagulans MTCC 5856 was added to 25 mL Aliquat 336 (50 % w/v in Oleyl alcohol) (Sigma Chemical Co., St Louis, MO, USA) and then stirred for 45 min.

Concentration Assay:

Article Title: Antibacterial and Antifungal Activities of Poloxamer Micelles Containing Ceragenin CSA-131 on Ciliated Tissues
Article Snippet: Well contents were removed in 1 mL of neutralizing broth (Dey-Engley, Sigma–Aldrich) and placed in a sonicating water bath (Fisher Scientific FS60, 42 kHz, 100 W, Pittsburg, PA, USA) to disrupt biofilms. .. The MBEC was defined as the lowest concentration of antibiotic that prevented bacterial regrowth [ ].

Article Title: 3-Substituted N-Benzylpyrazine-2-carboxamide Derivatives: Synthesis, Antimycobacterial and Antibacterial Evaluation
Article Snippet: The culturing medium was Middlebrook 7H9 (MB) broth (Sigma-Aldrich), enriched with 0.4% of glycerol (Sigma-Aldrich) and 10% of Middlebrook OADC growth supplement (Himedia, Mumbai, India). .. Tested compounds were dissolved in DMSO (Sigma-Aldrich), and the MB broth was then added to obtain a concentration of 2000 μg·mL−1 .

Article Title: Deficiency of the ferrous iron transporter FeoAB is linked with metronidazole resistance in Bacteroides fragilis
Article Snippet: Bacterial strains were grown at 37°C as described previously using brain heart infusion (BHI) media supplemented with 15 mg/L haemin (BHIS) for B. fragilis isolates in H. M. Wexler's laboratory (Anaerobe Systems, Morgan Hill, CA, USA) or BHI supplemented with 1 g/L l -cysteine, 5 mg/L haemin, 1 mg/L resazurin and 20 mL/L 10% NaHCO3 (BHIS-ER) in E. R. Rocha's laboratory and Luria–Bertani agar or broth (Sigma) for Escherichia coli DH10B. .. The concentration of haemin used in different laboratories is above the minimum required for B. fragilis growth.

Staining:

Article Title: Mycobacterium avium Infections of Acanthamoeba Strains: Host Strain Variability, Grazing-Acquired Infections, and Altered Dynamics of Inactivation with Monochloramine ▿ Strains: Host Strain Variability, Grazing-Acquired Infections, and Altered Dynamics of Inactivation with Monochloramine ▿ †
Article Snippet: Fresh isolates ( < 2 months) were passaged no more than three times and were determined to be free of endosymbionts and acid-fast stained structures, by the use of methods described previously ( ). .. Mycobacterium avium subsp. hominissuis 104 ( ) was cultured on Middlebrook 7H9/OADC (oleic acid-albumin-dextrose-catalase) broth (Sigma-Aldrich).

Article Title: Characterization of the AggR Regulon in Enteroaggregative Escherichia coli
Article Snippet: One milliliter of bacterial broth (OD600 , 0.8) was pelleted by centrifugation, washed in 1 ml of phosphate-buffered saline (PBS), and incubated overnight at 4°C in 2% paraformaldehyde (Sigma-Aldrich, St. Louis, MO), 2.5% glutaraldehyde, and 0.1 M CaCo buffer. .. The grids were then negatively stained with 2% uranyl acetate (Electron Microscopy Sciences Inc., Fort Washington, PA) for 5 s. The sample was blotted dry and viewed on a Jeol JEM1230 transmission electron microscope (TEM) (80 kV) at the Advanced Microscopy Laboratory at the University of Virginia (AML-UVA).

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  • 99
    Millipore bhi broth
    FTL_1753 is required for F. tularensis subsp. holarctica to survive hypoosmotic shock. (A and B) Survival data from downshock experiments performed in <t>BHI</t> broth. Bacteria were grown in BHI broth plus 300 mM <t>NaCl,</t> and then diluted in BHI broth plus 300, 200, 100, or 0 mM NaCl, producing hypoosmotic shocks of 0, 100, 200, and 300 mM, respectively. (C) Survival data for PBS-equilibrated bacteria diluted in water retrieved from a freshwater lake or double-distilled H 2 O. Statistical differences were determined by one-way analyses of variance (ANOVAs) with Dunnett's posttests. *, P
    Bhi Broth, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bhi broth/product/Millipore
    Average 99 stars, based on 15 article reviews
    Price from $9.99 to $1999.99
    bhi broth - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    85
    Millipore lgg culture broth lgg s
    <t>LGG</t> inhibits cytokine-induced apoptosis in intestinal epithelial cells kiKSR-expressing YAMC cells ( A–C ) or human colonic epithelial carcinoma cell line ( HT29 ) cells ( D ) were treated with TNF (100 ng/ml) or the “cytokine mixture” combination of TNF (100 ng/ml), IL-1 α (10 ng/ml), and γ -IFN (100 ng/ml), respectively, for 6 h in the presence or absence of a 1-h pretreatment with viable LGG, hk-LGG, or concentrated supernatant recovered from LGG culture MRS broth ( <t>LGG-s</t> ) at the concentrations indicated. Then, cells were fixed for terminal deoxynucleotidyltransferase TUNEL with apoptotic nuclei labeled with fluorescein isothiocyanate and DAPI staining ( A and D ). Fluorescein isothiocyanate- and DAPI-labeled images were taken from the same field. The percentage of cells undergoing apoptosis from a representative experiment is shown in B . Caspase activity in living cells was detected using a multi-caspase activity assay kit ( C ). Arrows indicate representative apoptotic nuclei. All experiments in this and subsequent figures were performed on at least three separate occasions.
    Lgg Culture Broth Lgg S, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lgg culture broth lgg s/product/Millipore
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    lgg culture broth lgg s - by Bioz Stars, 2020-04
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    92
    Millipore low free iron ssc broth cultures above
    Comparison of the S. maltophilia <t>siderophore</t> with salmochelin. (a) WT K279a bacteria were spread across the surface of a low-iron <t>SSC</t> plate and a paper disc placed in the centre of the plate was infused with deferrated SSC medium, supernatant obtained from K279a grown in SSC medium containing 400 µM DIP, or the indicated amount of purified salmochelin suspended in the SSC medium. After 3 days of incubation at 37 °C, bacterial growth was recorded. (b) Supernatants obtained from WT K279a grown in SSC medium containing 400 µM DIP or purified salmochelin suspended in the SSC medium were subjected to extraction by ethyl acetate, dichloromethane, or butanol, and then the levels of CAS reactivity in the aqueous phases before and after extraction were determined. The values presented are the means and standard deviations from triplicate samples. Asterisks indicates a significant difference between the before- and after-extraction readings (Student’s t -test; **, P
    Low Free Iron Ssc Broth Cultures Above, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/low free iron ssc broth cultures above/product/Millipore
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    low free iron ssc broth cultures above - by Bioz Stars, 2020-04
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    Image Search Results


    FTL_1753 is required for F. tularensis subsp. holarctica to survive hypoosmotic shock. (A and B) Survival data from downshock experiments performed in BHI broth. Bacteria were grown in BHI broth plus 300 mM NaCl, and then diluted in BHI broth plus 300, 200, 100, or 0 mM NaCl, producing hypoosmotic shocks of 0, 100, 200, and 300 mM, respectively. (C) Survival data for PBS-equilibrated bacteria diluted in water retrieved from a freshwater lake or double-distilled H 2 O. Statistical differences were determined by one-way analyses of variance (ANOVAs) with Dunnett's posttests. *, P

    Journal: Applied and Environmental Microbiology

    Article Title: A Single Mechanosensitive Channel Protects Francisella tularensis subsp. holarctica from Hypoosmotic Shock and Promotes Survival in the Aquatic Environment

    doi: 10.1128/AEM.02203-17

    Figure Lengend Snippet: FTL_1753 is required for F. tularensis subsp. holarctica to survive hypoosmotic shock. (A and B) Survival data from downshock experiments performed in BHI broth. Bacteria were grown in BHI broth plus 300 mM NaCl, and then diluted in BHI broth plus 300, 200, 100, or 0 mM NaCl, producing hypoosmotic shocks of 0, 100, 200, and 300 mM, respectively. (C) Survival data for PBS-equilibrated bacteria diluted in water retrieved from a freshwater lake or double-distilled H 2 O. Statistical differences were determined by one-way analyses of variance (ANOVAs) with Dunnett's posttests. *, P

    Article Snippet: For downshock experiments conducted in medium, single colonies of each strain were picked from MH chocolate agar plates and grown in BHI broth supplemented with 300 mM NaCl (EMD Chemicals).

    Techniques:

    LGG inhibits cytokine-induced apoptosis in intestinal epithelial cells kiKSR-expressing YAMC cells ( A–C ) or human colonic epithelial carcinoma cell line ( HT29 ) cells ( D ) were treated with TNF (100 ng/ml) or the “cytokine mixture” combination of TNF (100 ng/ml), IL-1 α (10 ng/ml), and γ -IFN (100 ng/ml), respectively, for 6 h in the presence or absence of a 1-h pretreatment with viable LGG, hk-LGG, or concentrated supernatant recovered from LGG culture MRS broth ( LGG-s ) at the concentrations indicated. Then, cells were fixed for terminal deoxynucleotidyltransferase TUNEL with apoptotic nuclei labeled with fluorescein isothiocyanate and DAPI staining ( A and D ). Fluorescein isothiocyanate- and DAPI-labeled images were taken from the same field. The percentage of cells undergoing apoptosis from a representative experiment is shown in B . Caspase activity in living cells was detected using a multi-caspase activity assay kit ( C ). Arrows indicate representative apoptotic nuclei. All experiments in this and subsequent figures were performed on at least three separate occasions.

    Journal: The Journal of biological chemistry

    Article Title: Probiotic Bacterium Prevents Cytokine-induced Apoptosis in Intestinal Epithelial Cells *

    doi: 10.1074/jbc.M207050200

    Figure Lengend Snippet: LGG inhibits cytokine-induced apoptosis in intestinal epithelial cells kiKSR-expressing YAMC cells ( A–C ) or human colonic epithelial carcinoma cell line ( HT29 ) cells ( D ) were treated with TNF (100 ng/ml) or the “cytokine mixture” combination of TNF (100 ng/ml), IL-1 α (10 ng/ml), and γ -IFN (100 ng/ml), respectively, for 6 h in the presence or absence of a 1-h pretreatment with viable LGG, hk-LGG, or concentrated supernatant recovered from LGG culture MRS broth ( LGG-s ) at the concentrations indicated. Then, cells were fixed for terminal deoxynucleotidyltransferase TUNEL with apoptotic nuclei labeled with fluorescein isothiocyanate and DAPI staining ( A and D ). Fluorescein isothiocyanate- and DAPI-labeled images were taken from the same field. The percentage of cells undergoing apoptosis from a representative experiment is shown in B . Caspase activity in living cells was detected using a multi-caspase activity assay kit ( C ). Arrows indicate representative apoptotic nuclei. All experiments in this and subsequent figures were performed on at least three separate occasions.

    Article Snippet: Supernatant recovered from LGG culture broth (LGG-s) was generated by centrifuging (1000 × g , 15 min) and filtering (0.2 μ m) LGG culture in MRS broth; then the filtrate was concentrated using Centricon Plus-20 (5–100 kDa, Millipore, Bedford, MA) by centrifugation (4000 × g , 1 h) following guidelines from the manufacturer.

    Techniques: Expressing, TUNEL Assay, Labeling, Staining, Activity Assay, Caspase Activity Assay

    Comparison of the S. maltophilia siderophore with salmochelin. (a) WT K279a bacteria were spread across the surface of a low-iron SSC plate and a paper disc placed in the centre of the plate was infused with deferrated SSC medium, supernatant obtained from K279a grown in SSC medium containing 400 µM DIP, or the indicated amount of purified salmochelin suspended in the SSC medium. After 3 days of incubation at 37 °C, bacterial growth was recorded. (b) Supernatants obtained from WT K279a grown in SSC medium containing 400 µM DIP or purified salmochelin suspended in the SSC medium were subjected to extraction by ethyl acetate, dichloromethane, or butanol, and then the levels of CAS reactivity in the aqueous phases before and after extraction were determined. The values presented are the means and standard deviations from triplicate samples. Asterisks indicates a significant difference between the before- and after-extraction readings (Student’s t -test; **, P

    Journal: Microbiology

    Article Title: Stenotrophomonas maltophilia produces an EntC-dependent catecholate siderophore that is distinct from enterobactin

    doi: 10.1099/mic.0.000545

    Figure Lengend Snippet: Comparison of the S. maltophilia siderophore with salmochelin. (a) WT K279a bacteria were spread across the surface of a low-iron SSC plate and a paper disc placed in the centre of the plate was infused with deferrated SSC medium, supernatant obtained from K279a grown in SSC medium containing 400 µM DIP, or the indicated amount of purified salmochelin suspended in the SSC medium. After 3 days of incubation at 37 °C, bacterial growth was recorded. (b) Supernatants obtained from WT K279a grown in SSC medium containing 400 µM DIP or purified salmochelin suspended in the SSC medium were subjected to extraction by ethyl acetate, dichloromethane, or butanol, and then the levels of CAS reactivity in the aqueous phases before and after extraction were determined. The values presented are the means and standard deviations from triplicate samples. Asterisks indicates a significant difference between the before- and after-extraction readings (Student’s t -test; **, P

    Article Snippet: In order to examine siderophore production by S. maltophilia , low-free-iron SSC broth cultures (above) were centrifuged at 3619 g for 30 min at 4 °C, and the resultant supernatants sterilized by passage through 0.22 µm syringe filters (EMD Millipore, Billerica, MA, USA).

    Techniques: Purification, Incubation

    Comparison of the S. maltophilia siderophore and enterobactin regarding their ability to stimulate bacterial growth and their extraction by organic solvents and migration on TLC. (a) S. typhimurium strain TA2700 was spread across the surface of a low-iron M9 minimal medium plate and a paper disc placed in the centre of the plate was infused with low-iron SSC medium, enterobactin suspended in the SSC medium and supernatant obtained from WT K279a grown in SSC medium containing 400 µM DIP. After 3 days of incubation at 37 °C, bacterial growth around the disc was recorded. (b) Supernatants obtained from WT K279a grown in SSC medium containing 400 µM DIP or purified enterobactin suspended in the SSC medium were subjected to extraction by ethyl acetate, dichloromethane, or butanol, after which the levels of CAS reactivity in the aqueous phases before and after extraction were determined. The values presented are the means and standard deviations from triplicate samples. Asterisks indicate a significant difference between the before- and after-extraction readings (Student’s t -test; **, P

    Journal: Microbiology

    Article Title: Stenotrophomonas maltophilia produces an EntC-dependent catecholate siderophore that is distinct from enterobactin

    doi: 10.1099/mic.0.000545

    Figure Lengend Snippet: Comparison of the S. maltophilia siderophore and enterobactin regarding their ability to stimulate bacterial growth and their extraction by organic solvents and migration on TLC. (a) S. typhimurium strain TA2700 was spread across the surface of a low-iron M9 minimal medium plate and a paper disc placed in the centre of the plate was infused with low-iron SSC medium, enterobactin suspended in the SSC medium and supernatant obtained from WT K279a grown in SSC medium containing 400 µM DIP. After 3 days of incubation at 37 °C, bacterial growth around the disc was recorded. (b) Supernatants obtained from WT K279a grown in SSC medium containing 400 µM DIP or purified enterobactin suspended in the SSC medium were subjected to extraction by ethyl acetate, dichloromethane, or butanol, after which the levels of CAS reactivity in the aqueous phases before and after extraction were determined. The values presented are the means and standard deviations from triplicate samples. Asterisks indicate a significant difference between the before- and after-extraction readings (Student’s t -test; **, P

    Article Snippet: In order to examine siderophore production by S. maltophilia , low-free-iron SSC broth cultures (above) were centrifuged at 3619 g for 30 min at 4 °C, and the resultant supernatants sterilized by passage through 0.22 µm syringe filters (EMD Millipore, Billerica, MA, USA).

    Techniques: Migration, Thin Layer Chromatography, Incubation, Purification

    Effect of temperature on S. maltophilia siderophore expression. Strain K279a was inoculated into SSC medium containing the indicated amounts of DIP, and then incubated at 37, 30, or 25 °C. Bacterial growth (left panels) and CAS reactivity (right panels) were measured. The values presented are the means and standard deviations from triplicate cultures, although small error bars are not always visible over the symbols. The data are representative of three independent experiments. Asterisks indicate points at which the OD or CAS activity of the cultures obtained at 37 °C were significantly greater than those of the cultures grown at 30 and/or 25 °C (Student’s t -test; *, P

    Journal: Microbiology

    Article Title: Stenotrophomonas maltophilia produces an EntC-dependent catecholate siderophore that is distinct from enterobactin

    doi: 10.1099/mic.0.000545

    Figure Lengend Snippet: Effect of temperature on S. maltophilia siderophore expression. Strain K279a was inoculated into SSC medium containing the indicated amounts of DIP, and then incubated at 37, 30, or 25 °C. Bacterial growth (left panels) and CAS reactivity (right panels) were measured. The values presented are the means and standard deviations from triplicate cultures, although small error bars are not always visible over the symbols. The data are representative of three independent experiments. Asterisks indicate points at which the OD or CAS activity of the cultures obtained at 37 °C were significantly greater than those of the cultures grown at 30 and/or 25 °C (Student’s t -test; *, P

    Article Snippet: In order to examine siderophore production by S. maltophilia , low-free-iron SSC broth cultures (above) were centrifuged at 3619 g for 30 min at 4 °C, and the resultant supernatants sterilized by passage through 0.22 µm syringe filters (EMD Millipore, Billerica, MA, USA).

    Techniques: Expressing, Incubation, Activity Assay

    Kinetics and iron-dependence of siderophore production by S. maltophilia . (a) Strain K279a bacteria were inoculated into SSC medium containing, as indicated, 0, 100, 200, or 400 µM DIP, and then incubated at 37 °C for 2 days. Bacterial growth was recorded spectrophotometrically (left y axis), and the CAS reactivity of culture supernatants, which is measured as net DHBA equivalents, was monitored (right y axis). The values presented are the means and standard deviations from triplicate cultures, although small error bars are not always visible over the symbols. The ODs of the cultures containing 400 µM DIP were significantly smaller than the others at all of the time points post-inoculation, whereas the growth of the cultures containing 200 µM DIP was smaller than that of the 0 and 100 µM DIP cultures at 24, 36 and 48 h (Student’s t -test; P

    Journal: Microbiology

    Article Title: Stenotrophomonas maltophilia produces an EntC-dependent catecholate siderophore that is distinct from enterobactin

    doi: 10.1099/mic.0.000545

    Figure Lengend Snippet: Kinetics and iron-dependence of siderophore production by S. maltophilia . (a) Strain K279a bacteria were inoculated into SSC medium containing, as indicated, 0, 100, 200, or 400 µM DIP, and then incubated at 37 °C for 2 days. Bacterial growth was recorded spectrophotometrically (left y axis), and the CAS reactivity of culture supernatants, which is measured as net DHBA equivalents, was monitored (right y axis). The values presented are the means and standard deviations from triplicate cultures, although small error bars are not always visible over the symbols. The ODs of the cultures containing 400 µM DIP were significantly smaller than the others at all of the time points post-inoculation, whereas the growth of the cultures containing 200 µM DIP was smaller than that of the 0 and 100 µM DIP cultures at 24, 36 and 48 h (Student’s t -test; P

    Article Snippet: In order to examine siderophore production by S. maltophilia , low-free-iron SSC broth cultures (above) were centrifuged at 3619 g for 30 min at 4 °C, and the resultant supernatants sterilized by passage through 0.22 µm syringe filters (EMD Millipore, Billerica, MA, USA).

    Techniques: Incubation

    Effect of entC on S. maltophilia growth and siderophore production. (a) Parental strain K279a (WT), entC mutant NUS8 ( entC ) and the complemented mutant NUS8 (pB entC ) ( entC/entC +) were inoculated into SSC medium containing 0, 100, 200, or 400 µM DIP and incubated at 37 °C for 48 h. At the indicated time points, the CAS reactivity in the culture supernatants was determined (left panels) and bacterial growth was measured spectrophotometrically (right panels). (b) Following 24 h of growth in SSC media containing 400 µM DIP, WT, entC mutant and complemented mutant supernatants were assessed for levels of reactivity in the Arnow assay (top) and the Rioux assay (bottom), expressed as a percentage of WT K279a activity. In (a, b), the values presented are the means and standard deviations from triplicate cultures, although small error bars are not always visible over the symbols. Asterisks indicate significant differences between the entC mutant and the WT and complemented strain (Student’s t -test; *, P

    Journal: Microbiology

    Article Title: Stenotrophomonas maltophilia produces an EntC-dependent catecholate siderophore that is distinct from enterobactin

    doi: 10.1099/mic.0.000545

    Figure Lengend Snippet: Effect of entC on S. maltophilia growth and siderophore production. (a) Parental strain K279a (WT), entC mutant NUS8 ( entC ) and the complemented mutant NUS8 (pB entC ) ( entC/entC +) were inoculated into SSC medium containing 0, 100, 200, or 400 µM DIP and incubated at 37 °C for 48 h. At the indicated time points, the CAS reactivity in the culture supernatants was determined (left panels) and bacterial growth was measured spectrophotometrically (right panels). (b) Following 24 h of growth in SSC media containing 400 µM DIP, WT, entC mutant and complemented mutant supernatants were assessed for levels of reactivity in the Arnow assay (top) and the Rioux assay (bottom), expressed as a percentage of WT K279a activity. In (a, b), the values presented are the means and standard deviations from triplicate cultures, although small error bars are not always visible over the symbols. Asterisks indicate significant differences between the entC mutant and the WT and complemented strain (Student’s t -test; *, P

    Article Snippet: In order to examine siderophore production by S. maltophilia , low-free-iron SSC broth cultures (above) were centrifuged at 3619 g for 30 min at 4 °C, and the resultant supernatants sterilized by passage through 0.22 µm syringe filters (EMD Millipore, Billerica, MA, USA).

    Techniques: Mutagenesis, Incubation, Activity Assay

    Effect of fepA on S. maltophilia siderophore utilization and production. (a) fepA mutant NUS10 ( fepA ) (top panel) and its complement NUS10 (pB fepA ) ( fepA/fepA+ ) (bottom panel) were spread across the surface of a low-iron SSC plate, and a disc in the centre of the plate was infused with low-iron SSC medium, FeCl 3 , or supernatant obtained from WT grown in SSC medium containing 400 µM DIP. After 3 days of incubation, bacterial growth was recorded. (b) Following 24 h of growth in SSC medium containing 400 µM DIP, WT, fepA mutant and complemented fepA mutant supernatants were assessed for levels of CAS reactivity, expressed as a percentage of WT K279a activity. The values presented are the means and standard deviations from triplicate cultures. Asterisks indicate a significant difference between the fepA mutant and the WT and complemented strain (Student’s t -test; *, P

    Journal: Microbiology

    Article Title: Stenotrophomonas maltophilia produces an EntC-dependent catecholate siderophore that is distinct from enterobactin

    doi: 10.1099/mic.0.000545

    Figure Lengend Snippet: Effect of fepA on S. maltophilia siderophore utilization and production. (a) fepA mutant NUS10 ( fepA ) (top panel) and its complement NUS10 (pB fepA ) ( fepA/fepA+ ) (bottom panel) were spread across the surface of a low-iron SSC plate, and a disc in the centre of the plate was infused with low-iron SSC medium, FeCl 3 , or supernatant obtained from WT grown in SSC medium containing 400 µM DIP. After 3 days of incubation, bacterial growth was recorded. (b) Following 24 h of growth in SSC medium containing 400 µM DIP, WT, fepA mutant and complemented fepA mutant supernatants were assessed for levels of CAS reactivity, expressed as a percentage of WT K279a activity. The values presented are the means and standard deviations from triplicate cultures. Asterisks indicate a significant difference between the fepA mutant and the WT and complemented strain (Student’s t -test; *, P

    Article Snippet: In order to examine siderophore production by S. maltophilia , low-free-iron SSC broth cultures (above) were centrifuged at 3619 g for 30 min at 4 °C, and the resultant supernatants sterilized by passage through 0.22 µm syringe filters (EMD Millipore, Billerica, MA, USA).

    Techniques: Mutagenesis, Incubation, Activity Assay