Journal: Bioscience Reports

Article Title: Metabolic profiling and combined therapeutic strategies unveil the cytotoxic potential of selenium-chrysin (SeChry) in NSCLC cells

doi: 10.1042/BSR20240752

Figure Lengend Snippet: ( A ) SeChry@PURE G4 -LA 24 nanoformulation. Chemical structures of the generation four polyurea dendrimer lactate conjugate and encapsulated SeChry molecules. A549, H292, PC-9, H1975, H522, H596 and BEAS-2B cells were exposed to empty nanoparticles (PURE G4 -LA) or SeChry@PURE G4 -LA 24 for 48 h. Percentage of cell death analyzed by flow cytometry using Annexin V and PI in ( B ) PURE G4 -LA 24 -treated cells and ( C ) Sechry@PURE G4 -LA 24 -treated cells. ( D ) EC50 curves and values for SeChry@PURE G4 -LA. Data are represented as mean ± SD. * P <0.5, ** P <0.01, *** P <0.001, **** P <0.0001 (one-way ANOVA with Tukey’s multiple comparisons test was used).

Article Snippet: Normal human bronchial epithelium cell line BEAS-2B (CRL-3588™, ATCC) was also used.

Techniques: Flow Cytometry

Journal: Bioscience Reports

Article Title: Metabolic profiling and combined therapeutic strategies unveil the cytotoxic potential of selenium-chrysin (SeChry) in NSCLC cells

doi: 10.1042/BSR20240752

Figure Lengend Snippet: Analysis of the expression profiles of MCT1/SLC16A1 in normal lung tissue and primary carcinomas in ( A ) LUAD and ( B ) LUSC and MCT4/SLC16A3 in ( C ) LUAD and ( D ) LUSC extracted from the TCGA database. A two-tailed unpaired Student’s t -test with Welch’s correction was used. ( E ) Cells were kept in control conditions and immunofluorescence analysis showed that all cell lines express MCT1. Nuclei were stained with DAPI (blue). The white bars scale means 20 μm. The cytotoxic response to SeChry@PURE G4 -LA 24 combined with MCT1 or MCT4 inhibitors was evaluated. Cells were incubated overnight with the MCT1 inhibitor AZD3965 and/or the MCT4 inhibitor AZD0095 and the following day, they were treated with SeChry@PURE G4 -LA 24 at the determined LC50 concentration. Percentage of cell death analyzed by flow cytometry using Annexin V and PI in ( F ) A549, ( G ) H292, ( H ) PC-9 and ( I ) BEAS-2B cells. Data are represented as mean ± SD. * P <0.5, ** P <0.01, *** P <0.001, **** P <0.0001 (one-way ANOVA with Tukey’s multiple comparisons test was used). The effect of the combination of SeChry@PURE G4 -LA 24 with the EGFR inhibitor gefitinib was evaluated. Cells, ( J ) A549, ( K ) H292, ( L ) PC-9, ( M ) H522, ( N ) H596, ( O ) H1975, and ( P ) BEAS, were incubated overnight with gefitinib and the following day, they were treated with SeChry@PURE G4 -LA 24 at the determined LC50 concentration. Data are represented as mean ± SD. * P <0.5, ** P <0.01, *** P <0.001, **** P <0.0001 (one-way ANOVA with Tukey’s multiple comparisons test was used).

Article Snippet: Normal human bronchial epithelium cell line BEAS-2B (CRL-3588™, ATCC) was also used.

Techniques: Expressing, Two Tailed Test, Control, Immunofluorescence, Staining, Incubation, Concentration Assay, Flow Cytometry

Journal: Molecules

Article Title: Cobalt(III)–Macrocyclic Scaffolds with Anti-Cancer Stem Cell Activity

doi: 10.3390/molecules29122743

Figure Lengend Snippet: ( A ) Representative dose–response curves for the treatment of HMLER, HMLER-shEcad, and BEAS-2B cells with 1 after 72 h incubation; and ( B ) representative dose–response curves for the treatment of HMLER, HMLER-shEcad, and BEAS-2B cells with 2 after 72 h incubation.

Article Snippet: The BEAS-2B bronchial epithelium cell line was acquired from American Type Culture Collection (ATCC, Manassas, VA, USA) and cultured in RPMI 1640 medium with 2 mM L-glutamine supplemented with 1% penicillin and 10% fetal bovine serum.

Techniques: Incubation

Journal: Oncology Research

Article Title: IL-17 induces NSCLC cell migration and invasion by elevating MMP19 gene transcription and expression through the interaction of p300-dependent STAT3-K631 acetylation and its Y705-phosphorylation

doi: 10.32604/or.2023.031053

Figure Lengend Snippet: Expression of IL-17RA and effects of IL-17 stimulation or IL-17RA knockdown on H1299 cell migration and invasion as well as p300, p-STAT3, Ack-STAT3, and MMP19 expression. (A and B) IL-17RA mRNA (A) and protein (B) in the three NSCLC cell lines and normal BEAS-2B cell lines were examined by RT-PCR and IB (** p < 0.01 vs . BEAS-2B). (C and D) H1299 cell migration (C) and invasion (D) stimulated with various concentrations (ng/mL) of IL-17 for 24 h were detected by wound-healing and Transwell assays (** p < 0.01 vs . 0 ng/mL). (E and F) H1299 cells were treated with IL-17 (50 ng/mL) or transfected with siIL-17RA for 48 h followed by IL-17 (50 ng/mL) for 24 h, and the cell migration (E) and invasion (F) were examined by the same assays (** p < 0.01 vs . DMEM; ▴▴ p < 0.01 vs . siCTR+IL-17). (G and H) Levels of p300, p-STAT3, STAT3, and MMP19 (G) or Ack-STAT3 (H) were measured using IB or IP/IB in H1299 cells exposed to 50 ng/mL IL-17 for different times (* p < 0.05, ** p < 0.01 vs . 0 h). (I and J) Levels of p300, p-STAT3, STAT3, and MMP19 (I) or Ack-STAT3 (J) in H1299 cells stimulated with IL-17 (50 ng/mL) or transfected with siIL-17RA plus IL-17 (50 ng/mL) for 3 h were assessed using IB or IP/IB (** p < 0.01 vs . DMEM; ▴▴ p < 0.01 vs . siCTR+IL-17). Representative pictures are shown. Data from three independent experiments are expressed as means ± SD and analyzed by one-way ANOVA (A–J).

Article Snippet: The human NSCLC cell lines such as NCI-H1299 (RRID: CVCL_0060), NCI-H1975 (RRID: CVCL_1511) or PC-9 (RRID: CVCL_B260), and normal bronchial epithelium cell line BEAS-2B (RRID: CVCL_0168) were purchased from American Type Culture Collection (ATCC) or European Collection of Authenticated Cell Cultures (ECACC).

Techniques: Expressing, Migration, Reverse Transcription Polymerase Chain Reaction, Transfection