bromouridine 5 triphosphate brutp  (Thermo Fisher)


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    Name:
    BrUPT 5 Bromouridine 5 Triphosphate 10 mM in TE buffer
    Description:
    BrUTP is an excellent substrate for RNA polymerase and has been used to monitor nucleolar transcription in situ BrUTP can be detected with anti BrdU antibodies
    Catalog Number:
    b21551
    Price:
    None
    Category:
    Oligos Primers Probes Nucleotides
    Applications:
    Cell Analysis|Cell Proliferation|Cell Viability, Proliferation & Function
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    Structured Review

    Thermo Fisher bromouridine 5 triphosphate brutp
    BrUTP is an excellent substrate for RNA polymerase and has been used to monitor nucleolar transcription in situ BrUTP can be detected with anti BrdU antibodies
    https://www.bioz.com/result/bromouridine 5 triphosphate brutp/product/Thermo Fisher
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bromouridine 5 triphosphate brutp - by Bioz Stars, 2021-03
    93/100 stars

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    Related Articles

    Generated:

    Article Title: Z Proteins of New World Arenaviruses Bind RIG-I and Interfere with Type I Interferon Induction ▿
    Article Snippet: Transfections were performed with either calcium phosphate or Lipofectamine 2000 (Invitrogen), according to the manufacturer's instructions. .. 5′-Triphosphate RNA (5′pppRNA) was generated from an 80-bp xenopus elongation factor 1α sequence cloned into pTRI by in vitro transcription using a T7 MEGAshortscript kit and purified using a MEGAclear kit (Ambion, Austin, TX). .. The cell monolayer was washed twice with ice-cold phosphate-buffered saline (PBS; Sigma-Aldrich) and RNA extracted using an RNeasy kit (Qiagen, Hilden, Germany).

    Sequencing:

    Article Title: Z Proteins of New World Arenaviruses Bind RIG-I and Interfere with Type I Interferon Induction ▿
    Article Snippet: Transfections were performed with either calcium phosphate or Lipofectamine 2000 (Invitrogen), according to the manufacturer's instructions. .. 5′-Triphosphate RNA (5′pppRNA) was generated from an 80-bp xenopus elongation factor 1α sequence cloned into pTRI by in vitro transcription using a T7 MEGAshortscript kit and purified using a MEGAclear kit (Ambion, Austin, TX). .. The cell monolayer was washed twice with ice-cold phosphate-buffered saline (PBS; Sigma-Aldrich) and RNA extracted using an RNeasy kit (Qiagen, Hilden, Germany).

    Clone Assay:

    Article Title: Z Proteins of New World Arenaviruses Bind RIG-I and Interfere with Type I Interferon Induction ▿
    Article Snippet: Transfections were performed with either calcium phosphate or Lipofectamine 2000 (Invitrogen), according to the manufacturer's instructions. .. 5′-Triphosphate RNA (5′pppRNA) was generated from an 80-bp xenopus elongation factor 1α sequence cloned into pTRI by in vitro transcription using a T7 MEGAshortscript kit and purified using a MEGAclear kit (Ambion, Austin, TX). .. The cell monolayer was washed twice with ice-cold phosphate-buffered saline (PBS; Sigma-Aldrich) and RNA extracted using an RNeasy kit (Qiagen, Hilden, Germany).

    In Vitro:

    Article Title: Z Proteins of New World Arenaviruses Bind RIG-I and Interfere with Type I Interferon Induction ▿
    Article Snippet: Transfections were performed with either calcium phosphate or Lipofectamine 2000 (Invitrogen), according to the manufacturer's instructions. .. 5′-Triphosphate RNA (5′pppRNA) was generated from an 80-bp xenopus elongation factor 1α sequence cloned into pTRI by in vitro transcription using a T7 MEGAshortscript kit and purified using a MEGAclear kit (Ambion, Austin, TX). .. The cell monolayer was washed twice with ice-cold phosphate-buffered saline (PBS; Sigma-Aldrich) and RNA extracted using an RNeasy kit (Qiagen, Hilden, Germany).

    Purification:

    Article Title: Z Proteins of New World Arenaviruses Bind RIG-I and Interfere with Type I Interferon Induction ▿
    Article Snippet: Transfections were performed with either calcium phosphate or Lipofectamine 2000 (Invitrogen), according to the manufacturer's instructions. .. 5′-Triphosphate RNA (5′pppRNA) was generated from an 80-bp xenopus elongation factor 1α sequence cloned into pTRI by in vitro transcription using a T7 MEGAshortscript kit and purified using a MEGAclear kit (Ambion, Austin, TX). .. The cell monolayer was washed twice with ice-cold phosphate-buffered saline (PBS; Sigma-Aldrich) and RNA extracted using an RNeasy kit (Qiagen, Hilden, Germany).

    Infection:

    Article Title: Development of an Insect Vector Cell Culture and RNA Interference System To Investigate the Functional Role of Fijivirus Replication Protein
    Article Snippet: VCMs growing on a coverslip and infected with SRBSDV were fixed in 4% paraformaldehyde 48 h postinoculation (p.i.), immunostained with P10-specific IgG conjugated to FITC (P10-FITC) and P9-1-specific IgG conjugated to rhodamine (P9-1–rhodamine), and then examined with a Leica TCS SP5 inverted confocal microscope, as described previously ( ). .. For determining whether the viroplasms are the sites of viral RNA synthesis, VCMs on a coverslip were either mock infected or infected with SRBSDV and then treated with actinomycin D (Sigma) for 1 h at 45 h p.i. and incubated with bromouridine 5′-triphosphate (BrUTP) for 2 h via the use of Cellfectin (Invitrogen). .. The samples were fixed 48 h p.i., stained with monoclonal anti-bromodeoxyuridine (anti-BrdU; Sigma), and then treated with anti-mouse IgG conjugated to FITC (Invitrogen) and P9-1–rhodamine for imaging with confocal microscopy, as described previously ( ).

    Incubation:

    Article Title: Development of an Insect Vector Cell Culture and RNA Interference System To Investigate the Functional Role of Fijivirus Replication Protein
    Article Snippet: VCMs growing on a coverslip and infected with SRBSDV were fixed in 4% paraformaldehyde 48 h postinoculation (p.i.), immunostained with P10-specific IgG conjugated to FITC (P10-FITC) and P9-1-specific IgG conjugated to rhodamine (P9-1–rhodamine), and then examined with a Leica TCS SP5 inverted confocal microscope, as described previously ( ). .. For determining whether the viroplasms are the sites of viral RNA synthesis, VCMs on a coverslip were either mock infected or infected with SRBSDV and then treated with actinomycin D (Sigma) for 1 h at 45 h p.i. and incubated with bromouridine 5′-triphosphate (BrUTP) for 2 h via the use of Cellfectin (Invitrogen). .. The samples were fixed 48 h p.i., stained with monoclonal anti-bromodeoxyuridine (anti-BrdU; Sigma), and then treated with anti-mouse IgG conjugated to FITC (Invitrogen) and P9-1–rhodamine for imaging with confocal microscopy, as described previously ( ).

    Article Title: New Model for the Genesis and Maturation of Viroplasms Induced by Fijiviruses in Insect Vector Cells
    Article Snippet: Infection was allowed to proceed for 34.5, 58.5, or 82.5 h, and cells were then treated for 30 min with actinomycin D (Sigma) to inhibit cellular RNA polymerase II. .. Cells were transfected with bromouridine 5′-triphosphate (BrUTP) in the presence of Cellfectin (Invitrogen), according to the manufacturer's instructions, and were incubated for 60 min before fixation and immunostaining. .. BrUTP-labeled viral RNAs were stained with anti-bromodeoxyuridine (BrdU) from mouse (Sigma), followed by anti-mouse IgG conjugated to rhodamine (Sigma).

    Article Title: A CSB-PAF1C axis restores processive transcription elongation after DNA damage repair
    Article Snippet: After this treatment, cells were either mock-treated or irradiated with UV-C light (7 J/m2 ). .. Cells were then incubated in conditioned media for different periods of time (0, 3, 8, 24h) before being incubated with 2 mM bromouridine (Bru) at 37°C for a 30 min. .. The cells were then lysed in TRIzol reagent (Invitrogen) and Bru-containing RNA isolated as previously described ( ). cDNA libraries were made from the Bru-labeled RNA using the Illumina TruSeq library kit and paired-end 151 bp sequenced using the Illumina NovaSeq platform at the University of Michigan DNA Sequencing Core.

    Transfection:

    Article Title: New Model for the Genesis and Maturation of Viroplasms Induced by Fijiviruses in Insect Vector Cells
    Article Snippet: Infection was allowed to proceed for 34.5, 58.5, or 82.5 h, and cells were then treated for 30 min with actinomycin D (Sigma) to inhibit cellular RNA polymerase II. .. Cells were transfected with bromouridine 5′-triphosphate (BrUTP) in the presence of Cellfectin (Invitrogen), according to the manufacturer's instructions, and were incubated for 60 min before fixation and immunostaining. .. BrUTP-labeled viral RNAs were stained with anti-bromodeoxyuridine (BrdU) from mouse (Sigma), followed by anti-mouse IgG conjugated to rhodamine (Sigma).

    Immunostaining:

    Article Title: New Model for the Genesis and Maturation of Viroplasms Induced by Fijiviruses in Insect Vector Cells
    Article Snippet: Infection was allowed to proceed for 34.5, 58.5, or 82.5 h, and cells were then treated for 30 min with actinomycin D (Sigma) to inhibit cellular RNA polymerase II. .. Cells were transfected with bromouridine 5′-triphosphate (BrUTP) in the presence of Cellfectin (Invitrogen), according to the manufacturer's instructions, and were incubated for 60 min before fixation and immunostaining. .. BrUTP-labeled viral RNAs were stained with anti-bromodeoxyuridine (BrdU) from mouse (Sigma), followed by anti-mouse IgG conjugated to rhodamine (Sigma).

    Polymerase Chain Reaction:

    Article Title: Hypothalamic–Pituitary–Thyroid Axis Crosstalk With the Hypothalamic–Pituitary–Gonadal Axis and Metabolic Regulation in the Eurasian Tree Sparrow During Mating and Non-mating Periods
    Article Snippet: The PCR primers ( ) were designed in the conserved regions of corresponding gene sequences deposited in GeneBank of other passerines such as zebra finch (Taeniopygia suttata ) and white-throated sparrow (Zonotrichia albicollis ) and obtained the open reading frames for Dio2, Dio3, TRH, TSH , and GnRH-I in ETSs (the nucleotide sequences for each gene were deposited in NCBI, ) by aligning the corresponding sequences using Primer Premier 5.0 (PREMIER Biosoft International, Palo Alto, CA, USA). .. All PCR amplifications were performed in a 50-μL reaction mixture containing 2.0 μL cDNA (200 ng/μL), 5.0 μL mixed dNTPs (2.5 mM each), 10.0 μL 5 × Fast Pfu Buffer and 2.0 μL forward and reverse primers (10 mM), respectively, 1 μL Trans Start FastPfu DNA polymerase (2.5 U/μL), and 28.0 μL sterile water. .. qPCR of Dio2, Dio3, TRH, TSH, GnRH-I , and GnIH for the ETS The qPCR reactions were set up using the TransStrat Top Green qPCR SuperMix (Quanshijin, Beijing, China).

    other:

    Article Title: An exo probe-based recombinase polymerase amplification assay for the rapid detection of porcine parvovirus.
    Article Snippet: Recombinase polymerase amplification (RPA), an isothermal amplification technology, has been developed as an alternative to PCR in pathogen detection.

    Article Title: A Proximity Ligation-Based Method to Detect RNA-DNA Association
    Article Snippet: 5-Bromouridine (BrU).

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  • 93
    Thermo Fisher 5 bromouridine bru
    5 Bromouridine Bru, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/5 bromouridine bru/product/Thermo Fisher
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    5 bromouridine bru - by Bioz Stars, 2021-03
    93/100 stars
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