polylysine  (Roche)


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    Structured Review

    Roche polylysine
    Polylysine, supplied by Roche, used in various techniques. Bioz Stars score: 92/100, based on 103 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polylysine/product/Roche
    Average 92 stars, based on 103 article reviews
    Price from $9.99 to $1999.99
    polylysine - by Bioz Stars, 2020-09
    92/100 stars

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    Related Articles

    TUNEL Assay:

    Article Title: Role of Estradiol in the Dynamic Control of Tanycyte Plasticity Mediated by Vascular Endothelial Cells in the Median Eminence
    Article Snippet: .. The in situ cell death detection terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling (TUNEL) kit was from Roche (Mannheim, Germany). .. Tanycytes and endothelial cells were isolated from the median eminence of the hypothalamus of 10-d-old rats, cultured, and characterized as described in our previous studies ( , ) and detailed in the Supplemental Methods published as supplemental data on The Endocrine Society’s Journals Online web site at http://endo.endojournals.org.

    Luciferase:

    Article Title: Acetylation of cytidine in messenger RNA promotes translation efficiency
    Article Snippet: .. Luciferase mRNA was transcribed by incubating 2 μg of linearized DNA, 1× transcription buffer (40 mM Tris-HCl [pH 8.0], 6 mM MgCl2 , 10 mM DTT, 2 mM spermidine), 1 mM each of ATP, GTP, and UTP with either ac4CTP or CTP, and 80 units of T7 RNA polymerase (Roche) in a final volume of 40 μL at 37°C for 2 hours. mRNA was then precipitated using 2.5 M lithium chloride at −20°C overnight. mRNA pellets were washed with 70% ethanol, air dried, then resuspended in H2 O to 1 μg/μL. mRNA integrity was assessed by running 1 μg of each mRNA on a 1% agarose gel. .. To evaluate the effect of wobble-ac4C on translation efficiency, a firefly luciferase construct lacking cytidines in wobble positions was generated.

    Agarose Gel Electrophoresis:

    Article Title: Acetylation of cytidine in messenger RNA promotes translation efficiency
    Article Snippet: .. Luciferase mRNA was transcribed by incubating 2 μg of linearized DNA, 1× transcription buffer (40 mM Tris-HCl [pH 8.0], 6 mM MgCl2 , 10 mM DTT, 2 mM spermidine), 1 mM each of ATP, GTP, and UTP with either ac4CTP or CTP, and 80 units of T7 RNA polymerase (Roche) in a final volume of 40 μL at 37°C for 2 hours. mRNA was then precipitated using 2.5 M lithium chloride at −20°C overnight. mRNA pellets were washed with 70% ethanol, air dried, then resuspended in H2 O to 1 μg/μL. mRNA integrity was assessed by running 1 μg of each mRNA on a 1% agarose gel. .. To evaluate the effect of wobble-ac4C on translation efficiency, a firefly luciferase construct lacking cytidines in wobble positions was generated.

    In Situ:

    Article Title: Role of Estradiol in the Dynamic Control of Tanycyte Plasticity Mediated by Vascular Endothelial Cells in the Median Eminence
    Article Snippet: .. The in situ cell death detection terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling (TUNEL) kit was from Roche (Mannheim, Germany). .. Tanycytes and endothelial cells were isolated from the median eminence of the hypothalamus of 10-d-old rats, cultured, and characterized as described in our previous studies ( , ) and detailed in the Supplemental Methods published as supplemental data on The Endocrine Society’s Journals Online web site at http://endo.endojournals.org.

    Mutagenesis:

    Article Title: Dissecting RNA folding by nucleotide analog interference mapping (NAIM)
    Article Snippet: .. It is supplied with a 5× transcription buffer and 0.1 M DTT stock solution 5× Mutant (Y639F) T7 RNA polymerase transcription buffer (EpiCentre) RNase inhibitor (40 U μl−1 ; Roche) Alkaline phosphatase (20 U μl−1 ; Roche) 10× Dephosphorylation buffer (Roche) T4-polynucleotide kinase (PNK, 10 U μl−1 ; NEB) 10× T4-PNK buffer (NEB) DNA polymerase I Klenow fragment exo− (2 U μl−1 ; Roche) 10× Nucleotide analog solution (Glen Research) NTPs (100 mM stock; Roche) γ-P32 -ATP (6,000 Ci mmol−1 ; Perkin Elmer) ! .. CAUTION Radiation can cause cell damage; wear protective gloves, goggles and lab coat and use a protective shield. α-P32 -dCTP (6,000 Ci mmol−1 ; Perkin Elmer) !

    Immunofluorescence:

    Article Title: Stress Induces Changes in the Phosphorylation of Trypanosoma cruzi RNA Polymerase II, Affecting Its Association with Chromatin and RNA Processing
    Article Snippet: .. The cells were incubated for 5 min at room temperature and then added to glass slides coated with polylysine, fixed for 15 min with 4% paraformaldehyde in PBS, and processed for immunofluorescence using 2.5 μg of mouse monoclonal anti-5-bromodeoxyridine (BrdU) antibody (Roche) per milliliter and anti-TcRNA Pol II diluted in 1% bovine serum albumin (BSA)–PBS. .. Bound antibodies were detected by using Alexa Fluor 594 goat anti-mouse IgG and Alexa Fluor 488 goat anti-rabbit IgG (Invitrogen).

    Article Title: Actively Transcribing RNA Polymerase II Concentrates on Spliced Leader Genes in the Nucleus of Trypanosoma cruzi
    Article Snippet: .. The cells were incubated for 15 min at 28°C and then added to glass slides coated with polylysine and processed for immunofluorescence using as primary antibodies 2.5 μg of a mouse monoclonal anti-5-bromodeoxyridine (BrdU) antibody (Roche) per ml and the anti-CTD diluted in PBS-1% BSA, followed by anti-mouse IgG Rhodamine conjugate (1:200; Santa Cruz) and anti-rabbit IgG fluorescein conjugate (Gibco). ..

    Incubation:

    Article Title: Stress Induces Changes in the Phosphorylation of Trypanosoma cruzi RNA Polymerase II, Affecting Its Association with Chromatin and RNA Processing
    Article Snippet: .. The cells were incubated for 5 min at room temperature and then added to glass slides coated with polylysine, fixed for 15 min with 4% paraformaldehyde in PBS, and processed for immunofluorescence using 2.5 μg of mouse monoclonal anti-5-bromodeoxyridine (BrdU) antibody (Roche) per milliliter and anti-TcRNA Pol II diluted in 1% bovine serum albumin (BSA)–PBS. .. Bound antibodies were detected by using Alexa Fluor 594 goat anti-mouse IgG and Alexa Fluor 488 goat anti-rabbit IgG (Invitrogen).

    Article Title: Actively Transcribing RNA Polymerase II Concentrates on Spliced Leader Genes in the Nucleus of Trypanosoma cruzi
    Article Snippet: .. The cells were incubated for 15 min at 28°C and then added to glass slides coated with polylysine and processed for immunofluorescence using as primary antibodies 2.5 μg of a mouse monoclonal anti-5-bromodeoxyridine (BrdU) antibody (Roche) per ml and the anti-CTD diluted in PBS-1% BSA, followed by anti-mouse IgG Rhodamine conjugate (1:200; Santa Cruz) and anti-rabbit IgG fluorescein conjugate (Gibco). ..

    De-Phosphorylation Assay:

    Article Title: Dissecting RNA folding by nucleotide analog interference mapping (NAIM)
    Article Snippet: .. It is supplied with a 5× transcription buffer and 0.1 M DTT stock solution 5× Mutant (Y639F) T7 RNA polymerase transcription buffer (EpiCentre) RNase inhibitor (40 U μl−1 ; Roche) Alkaline phosphatase (20 U μl−1 ; Roche) 10× Dephosphorylation buffer (Roche) T4-polynucleotide kinase (PNK, 10 U μl−1 ; NEB) 10× T4-PNK buffer (NEB) DNA polymerase I Klenow fragment exo− (2 U μl−1 ; Roche) 10× Nucleotide analog solution (Glen Research) NTPs (100 mM stock; Roche) γ-P32 -ATP (6,000 Ci mmol−1 ; Perkin Elmer) ! .. CAUTION Radiation can cause cell damage; wear protective gloves, goggles and lab coat and use a protective shield. α-P32 -dCTP (6,000 Ci mmol−1 ; Perkin Elmer) !

    End Labeling:

    Article Title: Role of Estradiol in the Dynamic Control of Tanycyte Plasticity Mediated by Vascular Endothelial Cells in the Median Eminence
    Article Snippet: .. The in situ cell death detection terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling (TUNEL) kit was from Roche (Mannheim, Germany). .. Tanycytes and endothelial cells were isolated from the median eminence of the hypothalamus of 10-d-old rats, cultured, and characterized as described in our previous studies ( , ) and detailed in the Supplemental Methods published as supplemental data on The Endocrine Society’s Journals Online web site at http://endo.endojournals.org.

    In Situ Hybridization:

    Article Title: Myelin Transcription Factor 1 (Myt1) Expression in Demyelinated Lesions of Rodent and Human CNS
    Article Snippet: .. Following in situ hybridization detection of Myt1 transcripts, sections were immunostained with anti-BrdU antibody directly conjugated with horseradish peroxidase (Roche, Indianapolis, IN) and detected with DAB (Vector Labs, Burlingame, CA). ..

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  • 85
    Roche bromo utp br utp
    Bromo Utp Br Utp, supplied by Roche, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bromo utp br utp/product/Roche
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bromo utp br utp - by Bioz Stars, 2020-09
    85/100 stars
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