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Thermo Fisher br utp
Br Utp, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/br utp/product/Thermo Fisher
Average 98 stars, based on 14 article reviews
Price from $9.99 to $1999.99
br utp - by Bioz Stars, 2021-01
98/100 stars

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Random Primed:

Article Title: Soluble Invertase Expression Is an Early Target of Drought Stress during the Critical, Abortion-Sensitive Phase of Young Ovary Development in Maize 1
Article Snippet: .. Ivr1 , Ivr2 , and Incw1 [32 P]ATP probes were produced with a random primed DNA-labeling kit (Roche Diagnostics) whereas for Incw2 , [32 P]UTP RNA probes were generated using a T7 phage polymerase RNA-labeling kit (Ambion Inc., Austin, TX). .. Membranes were hybridized for 20 h under conditions providing probe specific responses (0.25 m sodium phosphate, 7% [w/v] SDS, 1% [w/v] BSA, pH 7.2 at 65°C for the [32 P]ATP DNA probes, and 50% [v/v] formamide hybridization solution (Ultrahyb, Ambion Inc.) at 65°C for the [32 P]UTP RNA probe) and washed at high stringency (20 m m sodium phosphate, 5% [w/v] SDS, pH 7.2, and 20 m m sodium phosphate, 1% [w/v] SDS, pH 7.2, at 65°C for the [32 P]ATP DNA probes, and 2× SSC/0.1% [w/v] SDS, 0.1× SSC/0.1% [w/v] SDS at 68°C for the [32 P]UTP RNA probe).

Incubation:

Article Title: TUT7 controls the fate of precursor microRNAs by using three different uridylation mechanisms
Article Snippet: .. After 5 min of incubation, unbound His-rTUT7 molecules were flushed away with 100 μl imaging buffer A (0.5× buffer A substituted with, 0.1 mg/ml glucose oxidase (G2133, Sigma), 4 μg/ml catalase (10106810001, Roche), 1 mM Trolox ((±)-6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid, 238813, Sigma) and 0.1 mM UTP (18333-013, Ambion). .. Next, 1 nM labeled RNA substrate(s) was/were introduced in the chamber while imaging at room temperature (23 ± 1°C) to monitor the interaction of TUT7 with RNA in real time.

Article Title: A Land Plant-Specific Transcription Factor Directly Enhances Transcription of a Pathogenic Noncoding RNA Template by DNA-Dependent RNA Polymerase II [OPEN]
Article Snippet: .. Transcription reactions (20 μL) containing 50 mM HEPES-KOH, pH 7.9, 1 mM MnCl2 , 6 mM MgCl2 , 40 mM (NH4 )2 SO4 , 10% (v/v) glycerol, 20 unit of RNase inhibitor, 500 μM rATP, 500 μM rGTP, 500 μM rCTP, 500 μM rUTP, 10 ng/μL PSTVd, 4 μM protein, and 9 μL FLAG-Pol II were performed at 28 ° C for 4 h. The reactions were terminated by proteinase K (Invitrogen) treatment for 15 min at 37°C, followed by incubation at 95°C for 5 min. .. The transcription products were separated on 5% (w/v) polyacrylamide/8 M urea gel, transferred to Hybond-XL nylon membranes (Amersham Biosciences) by Trans-Blot SD semidry electrophoretic transfer cell (Bio-Rad), and immobilized by UV cross-linking.

Produced:

Article Title: Soluble Invertase Expression Is an Early Target of Drought Stress during the Critical, Abortion-Sensitive Phase of Young Ovary Development in Maize 1
Article Snippet: .. Ivr1 , Ivr2 , and Incw1 [32 P]ATP probes were produced with a random primed DNA-labeling kit (Roche Diagnostics) whereas for Incw2 , [32 P]UTP RNA probes were generated using a T7 phage polymerase RNA-labeling kit (Ambion Inc., Austin, TX). .. Membranes were hybridized for 20 h under conditions providing probe specific responses (0.25 m sodium phosphate, 7% [w/v] SDS, 1% [w/v] BSA, pH 7.2 at 65°C for the [32 P]ATP DNA probes, and 50% [v/v] formamide hybridization solution (Ultrahyb, Ambion Inc.) at 65°C for the [32 P]UTP RNA probe) and washed at high stringency (20 m m sodium phosphate, 5% [w/v] SDS, pH 7.2, and 20 m m sodium phosphate, 1% [w/v] SDS, pH 7.2, at 65°C for the [32 P]ATP DNA probes, and 2× SSC/0.1% [w/v] SDS, 0.1× SSC/0.1% [w/v] SDS at 68°C for the [32 P]UTP RNA probe).

Concentration Assay:

Article Title: Multiplex live single-cell transcriptional analysis demarcates cellular functional heterogeneity
Article Snippet: .. UTP was replaced with aminoallyl-UTP (Thermo Scientific) at the same concentration. .. 20U SUPERase In RNase Inhibitor (Thermo Scientific) was added to each reaction.

Generated:

Article Title: Soluble Invertase Expression Is an Early Target of Drought Stress during the Critical, Abortion-Sensitive Phase of Young Ovary Development in Maize 1
Article Snippet: .. Ivr1 , Ivr2 , and Incw1 [32 P]ATP probes were produced with a random primed DNA-labeling kit (Roche Diagnostics) whereas for Incw2 , [32 P]UTP RNA probes were generated using a T7 phage polymerase RNA-labeling kit (Ambion Inc., Austin, TX). .. Membranes were hybridized for 20 h under conditions providing probe specific responses (0.25 m sodium phosphate, 7% [w/v] SDS, 1% [w/v] BSA, pH 7.2 at 65°C for the [32 P]ATP DNA probes, and 50% [v/v] formamide hybridization solution (Ultrahyb, Ambion Inc.) at 65°C for the [32 P]UTP RNA probe) and washed at high stringency (20 m m sodium phosphate, 5% [w/v] SDS, pH 7.2, and 20 m m sodium phosphate, 1% [w/v] SDS, pH 7.2, at 65°C for the [32 P]ATP DNA probes, and 2× SSC/0.1% [w/v] SDS, 0.1× SSC/0.1% [w/v] SDS at 68°C for the [32 P]UTP RNA probe).

other:

Article Title: Oxazinomycin arrests RNA polymerase at the polythymidine sequences
Article Snippet: GreA facilitated RNA cleavage TEC were prepared by incubating the assembled TEC (1 μM) with 10 μM UTP and GTP or OZM triphosphate and GTP in TB10 for 3 min at 25°C and passed through Zeba™ Spin desalting columns 40K MWCO (Pierce Biotechnology, Rockford, USA) pre-equilibrated with TB0 buffer (40 mM HEPES-KOH pH 7.5, 80 mM KCl, 5% glycerol, 0.1 mM EDTA and 0.1 mM DTT).

Imaging:

Article Title: TUT7 controls the fate of precursor microRNAs by using three different uridylation mechanisms
Article Snippet: .. After 5 min of incubation, unbound His-rTUT7 molecules were flushed away with 100 μl imaging buffer A (0.5× buffer A substituted with, 0.1 mg/ml glucose oxidase (G2133, Sigma), 4 μg/ml catalase (10106810001, Roche), 1 mM Trolox ((±)-6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid, 238813, Sigma) and 0.1 mM UTP (18333-013, Ambion). .. Next, 1 nM labeled RNA substrate(s) was/were introduced in the chamber while imaging at room temperature (23 ± 1°C) to monitor the interaction of TUT7 with RNA in real time.

DNA Labeling:

Article Title: Soluble Invertase Expression Is an Early Target of Drought Stress during the Critical, Abortion-Sensitive Phase of Young Ovary Development in Maize 1
Article Snippet: .. Ivr1 , Ivr2 , and Incw1 [32 P]ATP probes were produced with a random primed DNA-labeling kit (Roche Diagnostics) whereas for Incw2 , [32 P]UTP RNA probes were generated using a T7 phage polymerase RNA-labeling kit (Ambion Inc., Austin, TX). .. Membranes were hybridized for 20 h under conditions providing probe specific responses (0.25 m sodium phosphate, 7% [w/v] SDS, 1% [w/v] BSA, pH 7.2 at 65°C for the [32 P]ATP DNA probes, and 50% [v/v] formamide hybridization solution (Ultrahyb, Ambion Inc.) at 65°C for the [32 P]UTP RNA probe) and washed at high stringency (20 m m sodium phosphate, 5% [w/v] SDS, pH 7.2, and 20 m m sodium phosphate, 1% [w/v] SDS, pH 7.2, at 65°C for the [32 P]ATP DNA probes, and 2× SSC/0.1% [w/v] SDS, 0.1× SSC/0.1% [w/v] SDS at 68°C for the [32 P]UTP RNA probe).

Modification:

Article Title: Enzymatic Incorporation of Emissive Pyrimidine Ribonucleotides
Article Snippet: .. Transcription reactions were performed in 40mM Tris-HCl buffer solution (pH 7.9) containing 250nM annealed template, 10mM MgCl2 , 10mM dithiothreitol (DTT), 10mM NaCl, 2mM spermidine, 1 UμL−1 RNase inhibitor (RiboLock), 1mM GTP, 1mM CTP (CTP=cytosine triphosphate), 1mM modified UTP 2 / 4 / 6 , 20μM ATP, 5 μCi α-32 P-ATP (800 Cimmole−1 stock) and 2.5 UμL−1 T7 RNA polymerase (Fermentas) in a total volume of 20 μL. .. In the control reaction 1mM UTP was used instead of modified UTPs.

Recombinase Polymerase Amplification:

Article Title: BDNF regulates eating behavior and locomotor activity in mice
Article Snippet: .. TrkB- (420 bp) and BDNF- (370 bp) specific [32 P]UTP-labeled probes (gift of Carlos Ibanez) were hybridized with 1 and 2 μg of total RNA, respectively, using a Hybspeed RPA kit (Ambion). ..

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