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Santa Cruz Biotechnology br utp
Br Utp, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 89/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/br utp/product/Santa Cruz Biotechnology
Average 89 stars, based on 3 article reviews
Price from $9.99 to $1999.99
br utp - by Bioz Stars, 2020-09
89/100 stars

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Isolation:

Article Title: RECQL5 Controls Transcript Elongation and Suppresses Genome Instability Associated with Transcription Stress
Article Snippet: .. Nuclear Run-On reactions were carried out with Br-UTP as described , and Br-UTP run-on labeled RNA was isolated using Br-UTP-specific antibody (IIB5, Santa Cruz). .. The purified RNA was used for the preparation of strand-specific RNA libraries using standard Illumina protocols, and sequenced on an Illumina GA IIX sequence analyzer.

Immunoprecipitation:

Article Title: Thyroid hormone receptor beta and NCOA4 regulate terminal erythrocyte differentiation
Article Snippet: .. To purify these Br-UTP–labeled nascent RNA, the fragmented NRO-RNA was immunoprecipitated with anti-BrdU agarose beads (Santa Cruz Biotechnology) in binding buffer (0.5× SSPE, 1 mM EDTA, 0.05% Tween) for 1–3 h at 4 °C with rotation. .. Subsequently, T4 PNK was used to repair the end of the immunoprecipitated BrU–NRO-RNA, at 37 °C for 1 h. The RNA was extracted and precipitated using acidic phenol–chloroform. cDNA synthesis was performed as previously published ( ) with few modifications.

Binding Assay:

Article Title: Thyroid hormone receptor beta and NCOA4 regulate terminal erythrocyte differentiation
Article Snippet: .. To purify these Br-UTP–labeled nascent RNA, the fragmented NRO-RNA was immunoprecipitated with anti-BrdU agarose beads (Santa Cruz Biotechnology) in binding buffer (0.5× SSPE, 1 mM EDTA, 0.05% Tween) for 1–3 h at 4 °C with rotation. .. Subsequently, T4 PNK was used to repair the end of the immunoprecipitated BrU–NRO-RNA, at 37 °C for 1 h. The RNA was extracted and precipitated using acidic phenol–chloroform. cDNA synthesis was performed as previously published ( ) with few modifications.

Labeling:

Article Title: RECQL5 Controls Transcript Elongation and Suppresses Genome Instability Associated with Transcription Stress
Article Snippet: .. Nuclear Run-On reactions were carried out with Br-UTP as described , and Br-UTP run-on labeled RNA was isolated using Br-UTP-specific antibody (IIB5, Santa Cruz). .. The purified RNA was used for the preparation of strand-specific RNA libraries using standard Illumina protocols, and sequenced on an Illumina GA IIX sequence analyzer.

Article Title: Pervasive Chromatin-RNA Binding Protein Interactions Enable RNA-Based Regulation of Transcription
Article Snippet: .. RNA was extracted with Trizol LS and nascent RNAs labeled with Br-UTP were purified on anti-BrdU agarose beads (Santa Cruz Biotechnology, sc-32323 AC). .. Purified nascent RNAs were treated with T4 polynucleotide kinase to remove phosphoryl group at 3′ end and add phoshoryl group at the 5′ end.

Purification:

Article Title: Pervasive Chromatin-RNA Binding Protein Interactions Enable RNA-Based Regulation of Transcription
Article Snippet: .. RNA was extracted with Trizol LS and nascent RNAs labeled with Br-UTP were purified on anti-BrdU agarose beads (Santa Cruz Biotechnology, sc-32323 AC). .. Purified nascent RNAs were treated with T4 polynucleotide kinase to remove phosphoryl group at 3′ end and add phoshoryl group at the 5′ end.

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    Santa Cruz Biotechnology br utp labeled nascent rna
    Br Utp Labeled Nascent Rna, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/br utp labeled nascent rna/product/Santa Cruz Biotechnology
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    Santa Cruz Biotechnology br utp
    Br Utp, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 89/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/br utp/product/Santa Cruz Biotechnology
    Average 89 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    br utp - by Bioz Stars, 2020-09
    89/100 stars
      Buy from Supplier

    90
    Santa Cruz Biotechnology br utp beads
    Br Utp Beads, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/br utp beads/product/Santa Cruz Biotechnology
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    br utp beads - by Bioz Stars, 2020-09
    90/100 stars
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