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Boehringer Mannheim br utp
Br Utp, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/br utp/product/Boehringer Mannheim
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
br utp - by Bioz Stars, 2021-03
86/100 stars

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Incubation:

Article Title: Association of Phosphatidylinositol 3-Kinase with Nuclear Transcription Sites in Higher Plants
Article Snippet: Sections were fixed in 4% (w/v) formaldehyde in PEM for 1 hr, washed, dried onto multiwell slides coated with glutaraldehyde-activated aminopropyltriethoxysilane, and permeabilized with 2% (w/v) cellulase Onozuka R10 in PBS. .. Incorporated BrUTP was detected by incubation with mouse anti–bromo-UTP antibodies (Boehringer Mannheim; diluted 1:20 [v/v] in PBS containing 3% [w/v] BSA), followed by incubation with donkey anti–mouse cy3 (Jackson Laboratories, Bar Harbor, ME; diluted 1:100 [v/v] in PBS containing 3% [w/v] BSA). ..

Activity Assay:

Article Title: Association of ATRX with pericentric heterochromatin and the Y chromosome of neonatal mouse spermatogonia
Article Snippet: Transcription run-on assays To determine subnuclear domain-specific transcriptional activity in neonatal spermatogonia, nascent transcripts were detected after 5-bromouridine 5'triphosphate (Br-UTP; Sigma), incorporation as described previously [ ]. .. Following a brief permeation of the plasma membrane with 0.03% Triton X (Biorad) in PBS for 2 minutes, the spermatogonial cell suspension was rinsed in fresh PBS and then transferred to transcription buffer supplemented as described [ ] for 40 minutes at 37°C before fixation in 4% Paraformaldehyde (PFA; EMS Hatfield, PA) supplemented with 0.15% Triton X. Transcriptional activity was detected with an anti-bromodeoxyuridine (BrdU, Boehringer Mannheim) antibody used at a concentration of 2 μg/ml followed by detection with an Alexa Fluor-488 secondary antibody (Molecular Probes). .. The nuclear domain corresponding to the Y chromosome in transcriptionally active cells was visualized by FISH while germ cells and somatic cells were subsequently distinguished by their corresponding 5-mC staining patterns as described above.

Concentration Assay:

Article Title: Association of ATRX with pericentric heterochromatin and the Y chromosome of neonatal mouse spermatogonia
Article Snippet: Transcription run-on assays To determine subnuclear domain-specific transcriptional activity in neonatal spermatogonia, nascent transcripts were detected after 5-bromouridine 5'triphosphate (Br-UTP; Sigma), incorporation as described previously [ ]. .. Following a brief permeation of the plasma membrane with 0.03% Triton X (Biorad) in PBS for 2 minutes, the spermatogonial cell suspension was rinsed in fresh PBS and then transferred to transcription buffer supplemented as described [ ] for 40 minutes at 37°C before fixation in 4% Paraformaldehyde (PFA; EMS Hatfield, PA) supplemented with 0.15% Triton X. Transcriptional activity was detected with an anti-bromodeoxyuridine (BrdU, Boehringer Mannheim) antibody used at a concentration of 2 μg/ml followed by detection with an Alexa Fluor-488 secondary antibody (Molecular Probes). .. The nuclear domain corresponding to the Y chromosome in transcriptionally active cells was visualized by FISH while germ cells and somatic cells were subsequently distinguished by their corresponding 5-mC staining patterns as described above.

Transfection:

Article Title: Large-Scale Chromatin Unfolding and Remodeling Induced by VP16 Acidic Activation Domain
Article Snippet: We followed the manufacturer's instructions, but used 1 μg of DNA and 5 μl FuGENE6 transfection reagent for a 35-mm petri dish. .. 2 d later, cells were transfected again with BrUTP using the DOTAP ( Boehringer Mannheim ) transfection reagent. .. 30 μl DOTAP was mixed with 70 μl Hepes, 20 mM FuGENE6, pH 7.4, and mixed with a 100-μl solution of 7.4 mM BrUTP in 20 mM Hepes, pH 7.4; the lipid–nucleotide complexes were allowed to form at room temperature for 15 min.

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    Boehringer Mannheim br utp
    Br Utp, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/br utp/product/Boehringer Mannheim
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    br utp - by Bioz Stars, 2021-03
    86/100 stars
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