br utp (Boehringer Mannheim)
86
Structured Review
Boehringer Mannheim
br utp
Br Utp, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/br utp/product/Boehringer Mannheim
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Br Utp, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/br utp/product/Boehringer Mannheim
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
br utp - by Bioz Stars,
2021-03
86/100 stars
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Incubation:Article Title: Association of Phosphatidylinositol 3-Kinase with Nuclear Transcription Sites in Higher Plants Article Snippet: Sections were fixed in 4% (w/v) formaldehyde in PEM for 1 hr, washed, dried onto multiwell slides coated with glutaraldehyde-activated aminopropyltriethoxysilane, and permeabilized with 2% (w/v) cellulase Onozuka R10 in PBS. .. Incorporated Activity Assay:Article Title: Association of ATRX with pericentric heterochromatin and the Y chromosome of neonatal mouse spermatogonia Article Snippet: Transcription run-on assays To determine subnuclear domain-specific transcriptional activity in neonatal spermatogonia, nascent transcripts were detected after 5-bromouridine 5'triphosphate (Br-UTP; Sigma), incorporation as described previously [ ]. .. Following a brief permeation of the plasma membrane with 0.03% Triton X (Biorad) in PBS for 2 minutes, the spermatogonial cell suspension was rinsed in fresh PBS and then transferred to transcription buffer supplemented as described [ ] for 40 minutes at 37°C before fixation in 4% Paraformaldehyde (PFA; EMS Hatfield, PA) supplemented with 0.15% Triton X. Transcriptional activity was detected with an Concentration Assay:Article Title: Association of ATRX with pericentric heterochromatin and the Y chromosome of neonatal mouse spermatogonia Article Snippet: Transcription run-on assays To determine subnuclear domain-specific transcriptional activity in neonatal spermatogonia, nascent transcripts were detected after 5-bromouridine 5'triphosphate (Br-UTP; Sigma), incorporation as described previously [ ]. .. Following a brief permeation of the plasma membrane with 0.03% Triton X (Biorad) in PBS for 2 minutes, the spermatogonial cell suspension was rinsed in fresh PBS and then transferred to transcription buffer supplemented as described [ ] for 40 minutes at 37°C before fixation in 4% Paraformaldehyde (PFA; EMS Hatfield, PA) supplemented with 0.15% Triton X. Transcriptional activity was detected with an Transfection:Article Title: Large-Scale Chromatin Unfolding and Remodeling Induced by VP16 Acidic Activation Domain Article Snippet: We followed the manufacturer's instructions, but used 1 μg of DNA and 5 μl FuGENE6 transfection reagent for a 35-mm petri dish. .. 2 d later, cells were transfected again with |