bp dna ladder  (Thermo Fisher)


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    Thermo Fisher bp dna ladder
    Identification of PPR virus and other co-infected organisms. (A) RT-PCR amplification of N gene of PPRV. Positive amplicons of 402 <t>bp</t> for PPR Virus. (B) RT-PCR amplification of L pro gene of FMD. Positive amplicons of 430 bp for FMD Virus. (C) PCR amplification of envelope gene of pox virus. Positive amplicons of 287 bp for pox virus. (D) PCR results for MTB. Positive amplicons of 372 bp for MTB. (E) PCR results for M. bovis . Positive amplicons of 600 bp for M. bovis . (F) PCR results for Klebsiella sp. infection. Positive amplicons of 411 bp for Klebsiella sp. L = 100 bp <t>DNA</t> <t>Ladder,</t> PC = Positive control, NC = Negative control, Field samples are indicated as 1 to 11.
    Bp Dna Ladder, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Identification of peste des petits ruminants virus along with co-infecting diseases of goats in Bangladesh"

    Article Title: Identification of peste des petits ruminants virus along with co-infecting diseases of goats in Bangladesh

    Journal: Journal of Advanced Veterinary and Animal Research

    doi: 10.5455/javar.2022.i615

    Identification of PPR virus and other co-infected organisms. (A) RT-PCR amplification of N gene of PPRV. Positive amplicons of 402 bp for PPR Virus. (B) RT-PCR amplification of L pro gene of FMD. Positive amplicons of 430 bp for FMD Virus. (C) PCR amplification of envelope gene of pox virus. Positive amplicons of 287 bp for pox virus. (D) PCR results for MTB. Positive amplicons of 372 bp for MTB. (E) PCR results for M. bovis . Positive amplicons of 600 bp for M. bovis . (F) PCR results for Klebsiella sp. infection. Positive amplicons of 411 bp for Klebsiella sp. L = 100 bp DNA Ladder, PC = Positive control, NC = Negative control, Field samples are indicated as 1 to 11.
    Figure Legend Snippet: Identification of PPR virus and other co-infected organisms. (A) RT-PCR amplification of N gene of PPRV. Positive amplicons of 402 bp for PPR Virus. (B) RT-PCR amplification of L pro gene of FMD. Positive amplicons of 430 bp for FMD Virus. (C) PCR amplification of envelope gene of pox virus. Positive amplicons of 287 bp for pox virus. (D) PCR results for MTB. Positive amplicons of 372 bp for MTB. (E) PCR results for M. bovis . Positive amplicons of 600 bp for M. bovis . (F) PCR results for Klebsiella sp. infection. Positive amplicons of 411 bp for Klebsiella sp. L = 100 bp DNA Ladder, PC = Positive control, NC = Negative control, Field samples are indicated as 1 to 11.

    Techniques Used: Infection, Reverse Transcription Polymerase Chain Reaction, Amplification, Polymerase Chain Reaction, Positive Control, Negative Control

    2) Product Images from "Pathological study and molecular detection of zoonotic diseases in small ruminants at slaughterhouses in Mymensingh, Bangladesh"

    Article Title: Pathological study and molecular detection of zoonotic diseases in small ruminants at slaughterhouses in Mymensingh, Bangladesh

    Journal: Veterinary World

    doi: 10.14202/vetworld.2022.2119-2130

    Examination of liver, spleen, and mesenteric lymph node (MLN) of Toxoplasma gondii- infected goat. (a) Necrotic lesions (arrows) were seen on liver. (b) Fibrinous covering (arrow) was seen on spleen (c) Swollen MLN was seen. (d) Multifocal portal granuloma (arrows) was seen which was characterized by infiltration of lymphocytes and macrophages (inset in higher magnification), congestion in a central vein (asterisk), as well as sinusoidal hemorrhages and pyknosis of hepatocytes (arrow heads), were seen. (e) Hyperplasia of trabeculae (arrows) of spleen was seen. (f) Lymphoid depletion (asterisks) with enlargement and branching of trabeculae (arrows) were seen. H E staining (d–f). (d–f = 10×, inset [d] = 100×). (g) PCR amplified products of 512 bp fragments of the B1 gene of T. gondii isolates from goats. L = DNA marker (100 bp), NC = Negative control, lane (8, 10) = representative T. gondii isolates from the liver and spleen of goat.
    Figure Legend Snippet: Examination of liver, spleen, and mesenteric lymph node (MLN) of Toxoplasma gondii- infected goat. (a) Necrotic lesions (arrows) were seen on liver. (b) Fibrinous covering (arrow) was seen on spleen (c) Swollen MLN was seen. (d) Multifocal portal granuloma (arrows) was seen which was characterized by infiltration of lymphocytes and macrophages (inset in higher magnification), congestion in a central vein (asterisk), as well as sinusoidal hemorrhages and pyknosis of hepatocytes (arrow heads), were seen. (e) Hyperplasia of trabeculae (arrows) of spleen was seen. (f) Lymphoid depletion (asterisks) with enlargement and branching of trabeculae (arrows) were seen. H E staining (d–f). (d–f = 10×, inset [d] = 100×). (g) PCR amplified products of 512 bp fragments of the B1 gene of T. gondii isolates from goats. L = DNA marker (100 bp), NC = Negative control, lane (8, 10) = representative T. gondii isolates from the liver and spleen of goat.

    Techniques Used: Infection, Staining, Polymerase Chain Reaction, Amplification, Marker, Negative Control

    Examination of liver of Coxiella burnetii- infected goat. (a) Hemorrhages (arrow) were seen on liver. (b) Extensive hemorrhages (arrow) were seen around the central vein of liver. (c-d) Extensive fibrin mimicking the necrotizing granuloma (arrows) was seen. (e and f) Typical fibrin ring (doughnut shape) granuloma (arrow) was seen, which was characterized by a centrally lipid vacuole surrounded by a fibrin ring accompanied by infiltrates with macrophages and lymphocytes. H E staining (b and c, e and f); Goldner’s trichrome stain (d). (b–e = 10×, f = 100×). (g) PCR amplified products of 687 bp fragments of the IS1111 gene of C. burnetii isolates from goats. L = DNA marker (100 bp), PC= Positive control, NC= Negative control, lane 1 = C. burnetii isolates from the liver of goat.
    Figure Legend Snippet: Examination of liver of Coxiella burnetii- infected goat. (a) Hemorrhages (arrow) were seen on liver. (b) Extensive hemorrhages (arrow) were seen around the central vein of liver. (c-d) Extensive fibrin mimicking the necrotizing granuloma (arrows) was seen. (e and f) Typical fibrin ring (doughnut shape) granuloma (arrow) was seen, which was characterized by a centrally lipid vacuole surrounded by a fibrin ring accompanied by infiltrates with macrophages and lymphocytes. H E staining (b and c, e and f); Goldner’s trichrome stain (d). (b–e = 10×, f = 100×). (g) PCR amplified products of 687 bp fragments of the IS1111 gene of C. burnetii isolates from goats. L = DNA marker (100 bp), PC= Positive control, NC= Negative control, lane 1 = C. burnetii isolates from the liver of goat.

    Techniques Used: Infection, Staining, Polymerase Chain Reaction, Amplification, Marker, Positive Control, Negative Control

    Examination of mesenteric lymph nodes (MLNs) of Listeria monocytogenes infected small ruminants. (a) Hemorrhages (arrows) were seen at the cut surface of MLN of goat. (b) Septic lymphadenitis (asterisks) and the presence of rod-shaped bacteria (circle) with infiltration of band neutrophils (inset in higher magnification) were seen in the MLN of goats. (c) Marked lymphoid depletion (asterisks) was seen in MLN of sheep. H E staining (b and c). (b = 10×, inset (b) and c = 40×). (d) PCR amplified products of 517 bp fragments of the Inlc gene of L. monocytogenes isolates from goats. L = DNA marker (100 bp), NC= Negative control, lanes 1–4 = representative L. monocytogenes isolates from the MLN of small ruminants.
    Figure Legend Snippet: Examination of mesenteric lymph nodes (MLNs) of Listeria monocytogenes infected small ruminants. (a) Hemorrhages (arrows) were seen at the cut surface of MLN of goat. (b) Septic lymphadenitis (asterisks) and the presence of rod-shaped bacteria (circle) with infiltration of band neutrophils (inset in higher magnification) were seen in the MLN of goats. (c) Marked lymphoid depletion (asterisks) was seen in MLN of sheep. H E staining (b and c). (b = 10×, inset (b) and c = 40×). (d) PCR amplified products of 517 bp fragments of the Inlc gene of L. monocytogenes isolates from goats. L = DNA marker (100 bp), NC= Negative control, lanes 1–4 = representative L. monocytogenes isolates from the MLN of small ruminants.

    Techniques Used: Infection, Staining, Polymerase Chain Reaction, Amplification, Marker, Negative Control

    Examination of the mesenteric lymph node (MLN), liver, and spleen of tuberculosis-infected small ruminants. (a) Calcifications (arrows) were seen at the cut surface of the MLN. (b) A caseous nodule (arrow) was seen on liver. (c) Semisolid caseous mass (arrow) was seen at the cut surface of the spleen. (d) Caseous necrosis (asterisk) surrounded by fibrous connective tissues (arrow) and infiltrated with inflammatory cells predominantly lymphocytes, macrophages and Langhan’s types giant cells (inset: Higher magnification) were seen in the MLN. (e) Calcification encapsulated by blue color fibrous connective tissue proliferation (arrow) was seen in the liver. (f) Pink color rod-shaped acid-fast bacilli (arrows) were seen in the caseous center of MLN. H E staining (d), Goldner’s trichrome staining (e), and acid-fast staining (f). (d-e = 10×; inset (d) and f = 100×). (g) PCR amplified products of 372 bp fragments of the 16S rRNA gene of Mycobacterium tuberculosis complex isolates from small ruminants. L = DNA marker (100 bp), PC = Positive control, NC = Negative control, lane 01–07 = representative M. tuberculosis complex isolates from the MLNs and liver of small ruminants. (h) PCR amplified products of 600 bp fragments of the MPB83 gene of Mycobacterium bovis isolates from small ruminants. L = DNA marker (100 bp), PC = Positive control, NC = Negative control, lanes 01–06 = representative M. bovis isolates from the liver and MLNs of small ruminants.
    Figure Legend Snippet: Examination of the mesenteric lymph node (MLN), liver, and spleen of tuberculosis-infected small ruminants. (a) Calcifications (arrows) were seen at the cut surface of the MLN. (b) A caseous nodule (arrow) was seen on liver. (c) Semisolid caseous mass (arrow) was seen at the cut surface of the spleen. (d) Caseous necrosis (asterisk) surrounded by fibrous connective tissues (arrow) and infiltrated with inflammatory cells predominantly lymphocytes, macrophages and Langhan’s types giant cells (inset: Higher magnification) were seen in the MLN. (e) Calcification encapsulated by blue color fibrous connective tissue proliferation (arrow) was seen in the liver. (f) Pink color rod-shaped acid-fast bacilli (arrows) were seen in the caseous center of MLN. H E staining (d), Goldner’s trichrome staining (e), and acid-fast staining (f). (d-e = 10×; inset (d) and f = 100×). (g) PCR amplified products of 372 bp fragments of the 16S rRNA gene of Mycobacterium tuberculosis complex isolates from small ruminants. L = DNA marker (100 bp), PC = Positive control, NC = Negative control, lane 01–07 = representative M. tuberculosis complex isolates from the MLNs and liver of small ruminants. (h) PCR amplified products of 600 bp fragments of the MPB83 gene of Mycobacterium bovis isolates from small ruminants. L = DNA marker (100 bp), PC = Positive control, NC = Negative control, lanes 01–06 = representative M. bovis isolates from the liver and MLNs of small ruminants.

    Techniques Used: Infection, Staining, Polymerase Chain Reaction, Amplification, Marker, Positive Control, Negative Control

    3) Product Images from "First report on the metabolic characterization of Sterigmatocystin production by select Aspergillus species from the Nidulantes section in Foeniculum vulgare"

    Article Title: First report on the metabolic characterization of Sterigmatocystin production by select Aspergillus species from the Nidulantes section in Foeniculum vulgare

    Journal: Frontiers in Microbiology

    doi: 10.3389/fmicb.2022.958424

    Electrophoretogram showing PCR amplification of transcribed genes in A. latus (MN791111). Lane 1–100 bp DNA ladder, Lane 2-Negative control PCR, Lane 3 through 9 includes amplicons for the aflR , laeA , pacC , fluG , flbA , pksA , and mtfA genes, sequentially.
    Figure Legend Snippet: Electrophoretogram showing PCR amplification of transcribed genes in A. latus (MN791111). Lane 1–100 bp DNA ladder, Lane 2-Negative control PCR, Lane 3 through 9 includes amplicons for the aflR , laeA , pacC , fluG , flbA , pksA , and mtfA genes, sequentially.

    Techniques Used: Polymerase Chain Reaction, Amplification, Negative Control

    Electrophoretogram showing PCR amplification of transcribed genes in E. quadrilineata (MN791104). Lane 1–100 bp DNA ladder, Lane 2-Negative control PCR, Lane 3 through 9 includes amplicons for the aflR , laeA , pacC , fluG , flbA , pksA , and mtfA genes, sequentially.
    Figure Legend Snippet: Electrophoretogram showing PCR amplification of transcribed genes in E. quadrilineata (MN791104). Lane 1–100 bp DNA ladder, Lane 2-Negative control PCR, Lane 3 through 9 includes amplicons for the aflR , laeA , pacC , fluG , flbA , pksA , and mtfA genes, sequentially.

    Techniques Used: Polymerase Chain Reaction, Amplification, Negative Control

    Electrophoretogram showing PCR amplification of transcribed genes in A. nidulans (MN791101). Lane 1–100 bp DNA ladder, Lane 2-Negative control PCR, Lane 3 through 9 includes amplicons for the aflR , laeA , pacC , fluG , flbA , pksA , and mtfA genes, sequentially.
    Figure Legend Snippet: Electrophoretogram showing PCR amplification of transcribed genes in A. nidulans (MN791101). Lane 1–100 bp DNA ladder, Lane 2-Negative control PCR, Lane 3 through 9 includes amplicons for the aflR , laeA , pacC , fluG , flbA , pksA , and mtfA genes, sequentially.

    Techniques Used: Polymerase Chain Reaction, Amplification, Negative Control

    Electrophoretogram showing PCR amplification of transcribed genes in E. quadrilineata (MN791105). Lane 1–100 bp DNA ladder, Lane 2-Negative control PCR, Lane 3 through 9 includes amplicons for the aflR , laeA , pacC , fluG , flbA , pksA , and mtfA genes, sequentially.
    Figure Legend Snippet: Electrophoretogram showing PCR amplification of transcribed genes in E. quadrilineata (MN791105). Lane 1–100 bp DNA ladder, Lane 2-Negative control PCR, Lane 3 through 9 includes amplicons for the aflR , laeA , pacC , fluG , flbA , pksA , and mtfA genes, sequentially.

    Techniques Used: Polymerase Chain Reaction, Amplification, Negative Control

    Electrophoretogram showing PCR amplification of transcribed genes in A. latus (MN791110). Lane 1–100 bp DNA ladder, Lane 2-Negative control PCR, Lane 3 through 9 includes amplicons for the aflR , laeA , pacC , fluG , flbA , pksA , and mtfA genes, sequentially.
    Figure Legend Snippet: Electrophoretogram showing PCR amplification of transcribed genes in A. latus (MN791110). Lane 1–100 bp DNA ladder, Lane 2-Negative control PCR, Lane 3 through 9 includes amplicons for the aflR , laeA , pacC , fluG , flbA , pksA , and mtfA genes, sequentially.

    Techniques Used: Polymerase Chain Reaction, Amplification, Negative Control

    4) Product Images from "Development of multiplex PCR assay for species-specific detection and identification of Saprolegnia parasitica"

    Article Title: Development of multiplex PCR assay for species-specific detection and identification of Saprolegnia parasitica

    Journal: Biotechnology Reports

    doi: 10.1016/j.btre.2022.e00758

    Multiplex PCR using genomic DNA from Saprolegnia parasitica simulated fish pond water. The amplification was observed up to 50 pg of genomic DNA in lane 3. M: 100 bp marker. Lane 10: negative control without template.
    Figure Legend Snippet: Multiplex PCR using genomic DNA from Saprolegnia parasitica simulated fish pond water. The amplification was observed up to 50 pg of genomic DNA in lane 3. M: 100 bp marker. Lane 10: negative control without template.

    Techniques Used: Multiplex Assay, Polymerase Chain Reaction, Fluorescence In Situ Hybridization, Amplification, Marker, Negative Control

    PCR amplification of rDNA ITS region (A) and hypothetical protein gene (B) of different Saprolegnia species. M: 100 bp DNA ladder. A-All the samples had amplicons of rDNA-ITS region at ∼750 bp. B- Saprolegnia parasitica (P) produced amplicons at ∼365 bp but no amplification in other (O) Saprolegnia species.
    Figure Legend Snippet: PCR amplification of rDNA ITS region (A) and hypothetical protein gene (B) of different Saprolegnia species. M: 100 bp DNA ladder. A-All the samples had amplicons of rDNA-ITS region at ∼750 bp. B- Saprolegnia parasitica (P) produced amplicons at ∼365 bp but no amplification in other (O) Saprolegnia species.

    Techniques Used: Polymerase Chain Reaction, Amplification, Produced

    rDNA-ITS region amplified with ITS1/ITS4 primers. M: 100 bp DNA ladder. A: Lane 1, 5,7, 13 and 14- amplicons of different Saprolegnia species (∼750 bp). Lane 2 and 3- Fusarium equiseti, lane 4- Schizophyllum commune, lane 6- Didymella heteroderae, lane 8- Emmia lacerate, lane 9 and lane 10- Schizophyllum species , lane 11- Phoma species, lane 12- Fusarium oxysporum . The fungal contaminants produced an amplicon size of ITS region varying from ∼550 to 650 bp.
    Figure Legend Snippet: rDNA-ITS region amplified with ITS1/ITS4 primers. M: 100 bp DNA ladder. A: Lane 1, 5,7, 13 and 14- amplicons of different Saprolegnia species (∼750 bp). Lane 2 and 3- Fusarium equiseti, lane 4- Schizophyllum commune, lane 6- Didymella heteroderae, lane 8- Emmia lacerate, lane 9 and lane 10- Schizophyllum species , lane 11- Phoma species, lane 12- Fusarium oxysporum . The fungal contaminants produced an amplicon size of ITS region varying from ∼550 to 650 bp.

    Techniques Used: Amplification, Produced

    Multiplex PCR using ITS1/ITS4 and Puf112/Puf310 primer pairs. M: 100 bp DNA ladder. A-Determination of detection limit of the protocol using two fold dilution (1 ng onwards) of genomic DNA. Amplification was observed up to 0.016 ng in lane 8. B- Evaluation of the protocol against different Saprolegnia species. Two bands of PCR product in S. parasitica (P) and single band in other Saprolegnia species (O).
    Figure Legend Snippet: Multiplex PCR using ITS1/ITS4 and Puf112/Puf310 primer pairs. M: 100 bp DNA ladder. A-Determination of detection limit of the protocol using two fold dilution (1 ng onwards) of genomic DNA. Amplification was observed up to 0.016 ng in lane 8. B- Evaluation of the protocol against different Saprolegnia species. Two bands of PCR product in S. parasitica (P) and single band in other Saprolegnia species (O).

    Techniques Used: Multiplex Assay, Polymerase Chain Reaction, Amplification

    5) Product Images from "Detection of kinase domain mutations in BCR::ABL1 leukemia by ultra-deep sequencing of genomic DNA"

    Article Title: Detection of kinase domain mutations in BCR::ABL1 leukemia by ultra-deep sequencing of genomic DNA

    Journal: Scientific Reports

    doi: 10.1038/s41598-022-17271-3

    Experimental design and quality metrics resulting for DNA-DeepNGS method. ( A ) Top: nine amplicon-scheme panel designed to cover the entire KD of BCR::ABL1 and the calculation of the intrinsic error; Bottom: bioinformatic pipeline. ( B ) Correlation of two replicates for the DNA-DeepNGS approach ( C ) ROC curve comparing the two methodologies. KD kinase domain, ROC receiver operating characteristic.
    Figure Legend Snippet: Experimental design and quality metrics resulting for DNA-DeepNGS method. ( A ) Top: nine amplicon-scheme panel designed to cover the entire KD of BCR::ABL1 and the calculation of the intrinsic error; Bottom: bioinformatic pipeline. ( B ) Correlation of two replicates for the DNA-DeepNGS approach ( C ) ROC curve comparing the two methodologies. KD kinase domain, ROC receiver operating characteristic.

    Techniques Used: Amplification

    Time course for the mutations measured by DNA-DeepNGS and RNA-NestedNGS, and BCR::ABL1 levels present in the most clinically relevant patients. Y-axis represents the value of RNA-NestedNGS VAF corrected by the ratio BCR::ABL1 (green), DNA-DeepNGS VAF (blue) or ratio BCR::ABL1 / ABL1 (red). ALL acute lymphoblastic leukemia, Asci asciminib, B bosutinib, CML chronic myeloid leukemia, D dasatinib, I imatinib, N nilotinib, Niv nivolumab, P ponatinib, R relapse, VAF variant allele frequency.
    Figure Legend Snippet: Time course for the mutations measured by DNA-DeepNGS and RNA-NestedNGS, and BCR::ABL1 levels present in the most clinically relevant patients. Y-axis represents the value of RNA-NestedNGS VAF corrected by the ratio BCR::ABL1 (green), DNA-DeepNGS VAF (blue) or ratio BCR::ABL1 / ABL1 (red). ALL acute lymphoblastic leukemia, Asci asciminib, B bosutinib, CML chronic myeloid leukemia, D dasatinib, I imatinib, N nilotinib, Niv nivolumab, P ponatinib, R relapse, VAF variant allele frequency.

    Techniques Used: Variant Assay

    6) Product Images from "Genetic and Morphological Diversity Assessment of Five Kalanchoe Genotypes by SCoT, ISSR and RAPD-PCR Markers"

    Article Title: Genetic and Morphological Diversity Assessment of Five Kalanchoe Genotypes by SCoT, ISSR and RAPD-PCR Markers

    Journal: Plants

    doi: 10.3390/plants11131722

    DNA fragment patterns of RAPD, ISSR and SCoT-PCR amplification of five kalanchoe genotypes. ( A ) RAPD-PCR amplification using primers OPA 2, OPA 7, OPA 9 and OPA 10, respectively. ( B ) ISSR-PCR amplification using primers ISSR-3, ISSR-5, ISSR-8 and ISSR-10, respectively. ( C ) SCoT-PCR amplification using primers SCoT3, SCoT11, SCoT13 and SCoT14, respectively. M = 100 bp Plus DNA Ladder. The monomorphic loci are presented in green numbers and the polymorphic loci are presented in yellow numbers.
    Figure Legend Snippet: DNA fragment patterns of RAPD, ISSR and SCoT-PCR amplification of five kalanchoe genotypes. ( A ) RAPD-PCR amplification using primers OPA 2, OPA 7, OPA 9 and OPA 10, respectively. ( B ) ISSR-PCR amplification using primers ISSR-3, ISSR-5, ISSR-8 and ISSR-10, respectively. ( C ) SCoT-PCR amplification using primers SCoT3, SCoT11, SCoT13 and SCoT14, respectively. M = 100 bp Plus DNA Ladder. The monomorphic loci are presented in green numbers and the polymorphic loci are presented in yellow numbers.

    Techniques Used: Polymerase Chain Reaction, Amplification

    7) Product Images from "Structural and Biophysical Characterization of Purified Recombinant Arabidopsis thaliana's Alternative Oxidase 1A (rAtAOX1A): Interaction With Inhibitor(s) and Activator"

    Article Title: Structural and Biophysical Characterization of Purified Recombinant Arabidopsis thaliana's Alternative Oxidase 1A (rAtAOX1A): Interaction With Inhibitor(s) and Activator

    Journal: Frontiers in Plant Science

    doi: 10.3389/fpls.2022.871208

    Cloning and expression of AtAOX1A in E. coli . (A) Polymerase chain reaction (PCR) amplification of 879 bp AtAOX1A using gene-specific primers. Lane 1, 50–1000 bp DNA ladder; lane 2, PCR amplified AtAOX1A (indicated with arrow). (B) Schematic diagram of the expression vector construction. The gene sequences were cloned into restriction endonuclease Eco RI and Xho I recognition sites of the pET28a vector to produce N-terminal His 6 -tag recombinant AtAOX1A protein. (C) Total protein of both E. coli /pET28a and E. coli /pAtAOX1A cells were separated on 12.5% (w/v) sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) for recombinant protein expression analysis. Each well was loaded with 40 μg of protein. Lane 1, 10–180 kDa protein marker; lane 2, E. coli /pET28a; lane 3, E. coli /pET28a induced with 0.1 mM IPTG; lane 4, E. coli /pAtAOX1A; lane 5, E. coli /pAtAOX1A induced with 0.1 mM IPTG. The induced rAtAOX1A is indicated with an arrow.
    Figure Legend Snippet: Cloning and expression of AtAOX1A in E. coli . (A) Polymerase chain reaction (PCR) amplification of 879 bp AtAOX1A using gene-specific primers. Lane 1, 50–1000 bp DNA ladder; lane 2, PCR amplified AtAOX1A (indicated with arrow). (B) Schematic diagram of the expression vector construction. The gene sequences were cloned into restriction endonuclease Eco RI and Xho I recognition sites of the pET28a vector to produce N-terminal His 6 -tag recombinant AtAOX1A protein. (C) Total protein of both E. coli /pET28a and E. coli /pAtAOX1A cells were separated on 12.5% (w/v) sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) for recombinant protein expression analysis. Each well was loaded with 40 μg of protein. Lane 1, 10–180 kDa protein marker; lane 2, E. coli /pET28a; lane 3, E. coli /pET28a induced with 0.1 mM IPTG; lane 4, E. coli /pAtAOX1A; lane 5, E. coli /pAtAOX1A induced with 0.1 mM IPTG. The induced rAtAOX1A is indicated with an arrow.

    Techniques Used: Clone Assay, Expressing, Polymerase Chain Reaction, Amplification, Plasmid Preparation, Recombinant, Polyacrylamide Gel Electrophoresis, SDS Page, Marker

    8) Product Images from "mir-146a genetic polymorphisms in systemic lupus erythematosus patients: Correlation with disease manifestations"

    Article Title: mir-146a genetic polymorphisms in systemic lupus erythematosus patients: Correlation with disease manifestations

    Journal: Non-coding RNA Research

    doi: 10.1016/j.ncrna.2022.05.001

    miR-146a (rs57095329 A/G) was detected by PCR-RFLP. PCR product after digestion with MSpI enzyme. Lane (1) 50 bp DNA ladder. Sample lanes (5 and 6): CG heterozygous genotype. Sample lanes (2, 3,4, and 7 to 10): AA wild genotype. All experiments were performed on 104 controls and 113 SLE patients.
    Figure Legend Snippet: miR-146a (rs57095329 A/G) was detected by PCR-RFLP. PCR product after digestion with MSpI enzyme. Lane (1) 50 bp DNA ladder. Sample lanes (5 and 6): CG heterozygous genotype. Sample lanes (2, 3,4, and 7 to 10): AA wild genotype. All experiments were performed on 104 controls and 113 SLE patients.

    Techniques Used: Polymerase Chain Reaction

    miR-146a (rs2431697C/T) was detected by PCR-RFLP. PCR product after digestion with Taq I enzyme. Lane (1) 50 bp DNA ladder. Sample lanes (6, 8, 9, 12, 16, and 17): CT heterozygous genotype. Sample lanes (3, 5, 7, and 10): wild homozygous CC genotype. Sample lanes (4, 11, and 13 to 15): mutant homozygous TT genotype. All experiments were performed on 104 controls and 113 SLE patients.
    Figure Legend Snippet: miR-146a (rs2431697C/T) was detected by PCR-RFLP. PCR product after digestion with Taq I enzyme. Lane (1) 50 bp DNA ladder. Sample lanes (6, 8, 9, 12, 16, and 17): CT heterozygous genotype. Sample lanes (3, 5, 7, and 10): wild homozygous CC genotype. Sample lanes (4, 11, and 13 to 15): mutant homozygous TT genotype. All experiments were performed on 104 controls and 113 SLE patients.

    Techniques Used: Polymerase Chain Reaction, Mutagenesis

    miR-146a (2910164C/G) was detected by PCR-RFLP. PCR product after digestion with Sac I enzyme. Lane (1) 50 bp DNA ladder. Sample lanes (2, 3, 4, 11, and 14): CG heterozygous genotype. Sample lanes (5, 6,7, and 10): wild homozygous CC genotype. Sample lanes (8, 9, 12, and 13): mutant homozygous GG genotype. All experiments were performed on 104 controls and 113 SLE patients.
    Figure Legend Snippet: miR-146a (2910164C/G) was detected by PCR-RFLP. PCR product after digestion with Sac I enzyme. Lane (1) 50 bp DNA ladder. Sample lanes (2, 3, 4, 11, and 14): CG heterozygous genotype. Sample lanes (5, 6,7, and 10): wild homozygous CC genotype. Sample lanes (8, 9, 12, and 13): mutant homozygous GG genotype. All experiments were performed on 104 controls and 113 SLE patients.

    Techniques Used: Polymerase Chain Reaction, Mutagenesis

    9) Product Images from "Association of Variant rs7903146(c/t) Single Nucleotide Polymorphism of Transcription Factor 7-like 2 Gene with Newly Detected Hyperglycemia in Pregnancy"

    Article Title: Association of Variant rs7903146(c/t) Single Nucleotide Polymorphism of Transcription Factor 7-like 2 Gene with Newly Detected Hyperglycemia in Pregnancy

    Journal: Indian Journal of Endocrinology and Metabolism

    doi: 10.4103/ijem.ijem_511_21

    Representative gel picture showing different genotypes of TCF7L2 gene polymorphism. Lane 1 is 50 bp DNA ladder; Lane 2, 3, 6, and 9 showing CT genotype; Lane 4, 7, 10, and 11 showing CC genotype; and Lane 5 and 8 showing TT genotype
    Figure Legend Snippet: Representative gel picture showing different genotypes of TCF7L2 gene polymorphism. Lane 1 is 50 bp DNA ladder; Lane 2, 3, 6, and 9 showing CT genotype; Lane 4, 7, 10, and 11 showing CC genotype; and Lane 5 and 8 showing TT genotype

    Techniques Used:

    10) Product Images from "Long‐term nitrogen enrichment mediates the effects of nitrogen supply and co‐inoculation on a viral pathogen). Long‐term nitrogen enrichment mediates the effects of nitrogen supply and co‐inoculation on a viral pathogen"

    Article Title: Long‐term nitrogen enrichment mediates the effects of nitrogen supply and co‐inoculation on a viral pathogen). Long‐term nitrogen enrichment mediates the effects of nitrogen supply and co‐inoculation on a viral pathogen

    Journal: Ecology and Evolution

    doi: 10.1002/ece3.8450

    An example gel electrophoresis used to evaluate infections of BYDV‐PAV and CYDV‐RPV following RT‐PCR. A 100 bp DNA ladder is on either side of the samples and controls. BYDV‐PAV is detected at 298 bp and CYDV‐RPV is detected at 447 bp. Labels for experimental samples consist of three letters and a number and follow the format: N supply rate (C = low N, N = high N), field soil inoculation (S = noninoculated growing medium, A = 0 kg N ha −1 year −1 , D = 34 kg N ha −1 year −1 , H = 272 kg N ha −1 yr −1 ), virus inoculation (C = co‐inoculation, H = mock‐inoculation, P = BYDV‐PAV, R = CYDV‐RPV), and replicate number. There are positive controls for BYDV‐PAV (PAV ctrl) and CYDV‐RPV (RPV ctrl). There are negative controls for noninoculated A . sativa (HO ctrl = healthy oat control), reverse transcription components (RT ctrl) and PCR components (PCR ctrl)
    Figure Legend Snippet: An example gel electrophoresis used to evaluate infections of BYDV‐PAV and CYDV‐RPV following RT‐PCR. A 100 bp DNA ladder is on either side of the samples and controls. BYDV‐PAV is detected at 298 bp and CYDV‐RPV is detected at 447 bp. Labels for experimental samples consist of three letters and a number and follow the format: N supply rate (C = low N, N = high N), field soil inoculation (S = noninoculated growing medium, A = 0 kg N ha −1 year −1 , D = 34 kg N ha −1 year −1 , H = 272 kg N ha −1 yr −1 ), virus inoculation (C = co‐inoculation, H = mock‐inoculation, P = BYDV‐PAV, R = CYDV‐RPV), and replicate number. There are positive controls for BYDV‐PAV (PAV ctrl) and CYDV‐RPV (RPV ctrl). There are negative controls for noninoculated A . sativa (HO ctrl = healthy oat control), reverse transcription components (RT ctrl) and PCR components (PCR ctrl)

    Techniques Used: Nucleic Acid Electrophoresis, Reverse Transcription Polymerase Chain Reaction, Polymerase Chain Reaction

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