Structured Review

GE Healthcare bovine serum albumin solution
Bovine Serum Albumin Solution, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/bovine serum albumin solution/product/GE Healthcare
Average 99 stars, based on 2 article reviews
Price from $9.99 to $1999.99
bovine serum albumin solution - by Bioz Stars, 2020-09
99/100 stars

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Conjugation Assay:

Article Title: Production of Native Bispecific Antibodies in Rabbits
Article Snippet: .. Conjugation of BSA to CNBr-activated Sepharose 4B (GE Healthcare, Amersham Biosciences, Sweden) was conducted according to the manufacturer's instructions. .. The conjugation of digoxin to ω-aminoalkyl Sepharose (GE Healthcare, Amersham Biosciences, Sweden) was conducted by a previously described method .

In Vivo:

Article Title: Immunogenicity of pulsatile-release PLGA microspheres for single-injection vaccination
Article Snippet: .. When prepared for in vivo use, PLGA and BSA solutions were filtered through 0.2 µm polytetrafluoroethylene filters (Whatman, Little Chalfont, England) prior to forming the emulsion and mixed in a sterile laminar flow hood. .. 2.3 Microsphere characterization Microsphere size distribution was determined using a Multisizer 3 Coulter Counter (Beckman Coulter, Brea, CA).

Concentration Assay:

Article Title: Interaction of C1q With Pentraxin 3 and IgM Revisited: Mutational Studies With Recombinant C1q Variants
Article Snippet: .. BSA and the C1q variants were diluted in 10 mM sodium acetate at the following concentration and pH: BSA, 25 μg/ml, pH 4.0; C1q variants, 50 μg/ml, pH 4.5 (wild-type) or 4.0 (C1q mutants), and immobilized on CM5 sensor chips (GE Healthcare) using the amine coupling chemistry in 10 mM Hepes, 150 mM NaCl, 3 mM EDTA, 0.005% surfactant P20, pH 7.4. .. Binding of C1q partners was measured at a flow rate of 20 μl/min in 50 mM Tris-HCl, 150 mM NaCl, 2 mM CaCl2 , 0.005% surfactant P20, pH 7.4.

Flow Cytometry:

Article Title: Immunogenicity of pulsatile-release PLGA microspheres for single-injection vaccination
Article Snippet: .. When prepared for in vivo use, PLGA and BSA solutions were filtered through 0.2 µm polytetrafluoroethylene filters (Whatman, Little Chalfont, England) prior to forming the emulsion and mixed in a sterile laminar flow hood. .. 2.3 Microsphere characterization Microsphere size distribution was determined using a Multisizer 3 Coulter Counter (Beckman Coulter, Brea, CA).

Immunoprecipitation:

Article Title: Amyloid-β Binds to the Extracellular Cysteine-rich Domain of Frizzled and Inhibits Wnt/β-Catenin Signaling
Article Snippet: .. Cross-linking —2.5 μ m Aβ40 was incubated for 2 h on ice with 0.5 μ m Fz5CRD in PBS buffer containing 0.5 μ m BSA in the absence or in the presence of 10 μ m cSP5, cross-linked with EDAC as previously described , and immunoprecipitated with anti-Aβ and protein A-Sepharose beads (Amersham Biosciences). .. After SDS-PAGE, the samples were transferred to nitrocellulose, and the membranes were probed with anti-Fz5CRD.

Incubation:

Article Title: Glucagon-Like Peptide-1 Induced Signaling and Insulin Secretion Do Not Drive Fuel and Energy Metabolism in Primary Rodent Pancreatic ?-Cells
Article Snippet: .. After 2 h incubation in 2.8 mM glucose-RPMI complete medium, islets were washed in KRBH/0.25% BSA and pre-incubated for 45 min at 37°C in KRBH/0.25% defatted BSA and 2.8 mM glucose after which they were incubated for 2 h (FA oxidation) or 4 h (FA esterification) in 1 ml KRBH/0.25% defatted BSA containing 2.8, 8.3 or 16.7 mM glucose in the presence or absence of 20 nM GLP-1, 0.1 mM (oxidation) or 0.2 mM (esterification) palmitate, 1 µCi/ml [9,10(n)-3 H] palmitate (51 Ci/mmol, GE Healthcare, Baie d'Urfé, QC, Canada), and 1 mM carnitine. .. At the end of the incubation, the media were collected for the determination of islet FA oxidation and total lipids were extracted from islets for the measurement of islet FA esterification .

Article Title: Amyloid-β Binds to the Extracellular Cysteine-rich Domain of Frizzled and Inhibits Wnt/β-Catenin Signaling
Article Snippet: .. Cross-linking —2.5 μ m Aβ40 was incubated for 2 h on ice with 0.5 μ m Fz5CRD in PBS buffer containing 0.5 μ m BSA in the absence or in the presence of 10 μ m cSP5, cross-linked with EDAC as previously described , and immunoprecipitated with anti-Aβ and protein A-Sepharose beads (Amersham Biosciences). .. After SDS-PAGE, the samples were transferred to nitrocellulose, and the membranes were probed with anti-Fz5CRD.

Western Blot:

Article Title: Biotin IgM Antibodies in Human Blood: A Previously Unknown Factor Eliciting False Results in Biotinylation-Based Immunoassays
Article Snippet: .. Western Blot Analysis For detection of biotin IgM by immunoblot, 1 µg/lane of biotinylated BSA (Bio-BSA) or BSA was separated by 10% SDS-PAGE and transferred onto nitrocellulose membranes (Whatman Protran BA 85), blocked in 5% BSA in Tris buffered saline containing 0.1% Tween 20 (TBST) for 30 min at room temperature (RT). ..

Recombinant:

Article Title: Poly( ADP‐ribose)‐mediated interplay of XPA and PARP1 leads to reciprocal regulation of protein function
Article Snippet: .. Briefly, for overlay assays using recombinant proteins, i.e., histone H1, BSA and XPA, in amounts as indicated, were subjected to 12% SDS/PAGE and transferred by wet blotting onto a nitrocellulose membrane (GE Healthcare, Little Chalfont, UK). .. Then, the membrane was incubated overnight at 4 °C either in TBST (150 mM NaCl, 10 mM Tris pH 8, 0.05% Tween 20) or in TBST containing 0.2 μm PAR.

SDS Page:

Article Title: Biotin IgM Antibodies in Human Blood: A Previously Unknown Factor Eliciting False Results in Biotinylation-Based Immunoassays
Article Snippet: .. Western Blot Analysis For detection of biotin IgM by immunoblot, 1 µg/lane of biotinylated BSA (Bio-BSA) or BSA was separated by 10% SDS-PAGE and transferred onto nitrocellulose membranes (Whatman Protran BA 85), blocked in 5% BSA in Tris buffered saline containing 0.1% Tween 20 (TBST) for 30 min at room temperature (RT). ..

Article Title: Poly( ADP‐ribose)‐mediated interplay of XPA and PARP1 leads to reciprocal regulation of protein function
Article Snippet: .. Briefly, for overlay assays using recombinant proteins, i.e., histone H1, BSA and XPA, in amounts as indicated, were subjected to 12% SDS/PAGE and transferred by wet blotting onto a nitrocellulose membrane (GE Healthcare, Little Chalfont, UK). .. Then, the membrane was incubated overnight at 4 °C either in TBST (150 mM NaCl, 10 mM Tris pH 8, 0.05% Tween 20) or in TBST containing 0.2 μm PAR.

Hood:

Article Title: Immunogenicity of pulsatile-release PLGA microspheres for single-injection vaccination
Article Snippet: .. When prepared for in vivo use, PLGA and BSA solutions were filtered through 0.2 µm polytetrafluoroethylene filters (Whatman, Little Chalfont, England) prior to forming the emulsion and mixed in a sterile laminar flow hood. .. 2.3 Microsphere characterization Microsphere size distribution was determined using a Multisizer 3 Coulter Counter (Beckman Coulter, Brea, CA).

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  • 99
    GE Healthcare bovine serum albumin solution
    Bovine Serum Albumin Solution, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bovine serum albumin solution/product/GE Healthcare
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    bovine serum albumin solution - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    92
    GE Healthcare bsa solution
    Bsa Solution, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    GE Healthcare tris bsa solution
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    GE Healthcare fitc bsa solution
    Fitc Bsa Solution, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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