bovine kidney  (Millipore)


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    Name:
    beta N Acetylglucosaminidase from bovine kidney
    Description:

    Catalog Number:
    a2415
    Price:
    None
    Applications:
    Bovine kidney β-N-acetylglucosaminidase is a lysosomal enzyme used to hydrolyze N-acetyl-β-D-glucosaminides and N-acetyl-β-Dgalactosaminides. It is used in chemoenzymatic synthesis of oligosaccharides based on their effective transglycosylation of β-GlcNAc and β-GalNAcc. It may be a useful tool to study Alzheimer's Disease .
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    Structured Review

    Millipore bovine kidney

    https://www.bioz.com/result/bovine kidney/product/Millipore
    Average 96 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    bovine kidney - by Bioz Stars, 2020-09
    96/100 stars

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    Related Articles

    High Performance Liquid Chromatography:

    Article Title: Galactosylated Fucose Epitopes in Nematodes
    Article Snippet: .. In general, a 1-μl aliquot of a HPLC fraction was mixed with 0.2 μl of exoglycosidase and 0.8 μl of 50 m m ammonium acetate solution, pH 5.0; after an overnight incubation at 37 °C, a 0.5-μl aliquot of the mixture was analyzed by MALDI-TOF MS. Exoglycosidases employed were: α-galactosidase from green coffee bean (Sigma, 11 milliunits), β-galactosidase from Aspergillus oryzae (Sigma, 27 milliunits) repurified by ion-exchange chromatography , α- l -fucosidase from bovine kidney (Sigma, 10 milliunits), β- N -acetylglucosaminidase from jack bean (Sigma, 6.25 milliunits), recombinant Xanthomonas manihotis α1–2/3-mannosidase (New England Biolabs, 6.4 units), and recombinant X. manihotis α1–6-mannosidase (New England Biolabs, 8 units). .. For removal of α1,3-linked fucose, glycan samples were dried in a SpeedVac and then incubated with 3 μl of 48% (v/v) hydrofluoric acid (HF) on ice for 24 h. The HF was allowed to evaporate overnight.

    Incubation:

    Article Title: Galactosylated Fucose Epitopes in Nematodes
    Article Snippet: .. In general, a 1-μl aliquot of a HPLC fraction was mixed with 0.2 μl of exoglycosidase and 0.8 μl of 50 m m ammonium acetate solution, pH 5.0; after an overnight incubation at 37 °C, a 0.5-μl aliquot of the mixture was analyzed by MALDI-TOF MS. Exoglycosidases employed were: α-galactosidase from green coffee bean (Sigma, 11 milliunits), β-galactosidase from Aspergillus oryzae (Sigma, 27 milliunits) repurified by ion-exchange chromatography , α- l -fucosidase from bovine kidney (Sigma, 10 milliunits), β- N -acetylglucosaminidase from jack bean (Sigma, 6.25 milliunits), recombinant Xanthomonas manihotis α1–2/3-mannosidase (New England Biolabs, 6.4 units), and recombinant X. manihotis α1–6-mannosidase (New England Biolabs, 8 units). .. For removal of α1,3-linked fucose, glycan samples were dried in a SpeedVac and then incubated with 3 μl of 48% (v/v) hydrofluoric acid (HF) on ice for 24 h. The HF was allowed to evaporate overnight.

    Recombinant:

    Article Title: Galactosylated Fucose Epitopes in Nematodes
    Article Snippet: .. In general, a 1-μl aliquot of a HPLC fraction was mixed with 0.2 μl of exoglycosidase and 0.8 μl of 50 m m ammonium acetate solution, pH 5.0; after an overnight incubation at 37 °C, a 0.5-μl aliquot of the mixture was analyzed by MALDI-TOF MS. Exoglycosidases employed were: α-galactosidase from green coffee bean (Sigma, 11 milliunits), β-galactosidase from Aspergillus oryzae (Sigma, 27 milliunits) repurified by ion-exchange chromatography , α- l -fucosidase from bovine kidney (Sigma, 10 milliunits), β- N -acetylglucosaminidase from jack bean (Sigma, 6.25 milliunits), recombinant Xanthomonas manihotis α1–2/3-mannosidase (New England Biolabs, 6.4 units), and recombinant X. manihotis α1–6-mannosidase (New England Biolabs, 8 units). .. For removal of α1,3-linked fucose, glycan samples were dried in a SpeedVac and then incubated with 3 μl of 48% (v/v) hydrofluoric acid (HF) on ice for 24 h. The HF was allowed to evaporate overnight.

    Ion Exchange Chromatography:

    Article Title: Galactosylated Fucose Epitopes in Nematodes
    Article Snippet: .. In general, a 1-μl aliquot of a HPLC fraction was mixed with 0.2 μl of exoglycosidase and 0.8 μl of 50 m m ammonium acetate solution, pH 5.0; after an overnight incubation at 37 °C, a 0.5-μl aliquot of the mixture was analyzed by MALDI-TOF MS. Exoglycosidases employed were: α-galactosidase from green coffee bean (Sigma, 11 milliunits), β-galactosidase from Aspergillus oryzae (Sigma, 27 milliunits) repurified by ion-exchange chromatography , α- l -fucosidase from bovine kidney (Sigma, 10 milliunits), β- N -acetylglucosaminidase from jack bean (Sigma, 6.25 milliunits), recombinant Xanthomonas manihotis α1–2/3-mannosidase (New England Biolabs, 6.4 units), and recombinant X. manihotis α1–6-mannosidase (New England Biolabs, 8 units). .. For removal of α1,3-linked fucose, glycan samples were dried in a SpeedVac and then incubated with 3 μl of 48% (v/v) hydrofluoric acid (HF) on ice for 24 h. The HF was allowed to evaporate overnight.

    Mass Spectrometry:

    Article Title: Galactosylated Fucose Epitopes in Nematodes
    Article Snippet: .. In general, a 1-μl aliquot of a HPLC fraction was mixed with 0.2 μl of exoglycosidase and 0.8 μl of 50 m m ammonium acetate solution, pH 5.0; after an overnight incubation at 37 °C, a 0.5-μl aliquot of the mixture was analyzed by MALDI-TOF MS. Exoglycosidases employed were: α-galactosidase from green coffee bean (Sigma, 11 milliunits), β-galactosidase from Aspergillus oryzae (Sigma, 27 milliunits) repurified by ion-exchange chromatography , α- l -fucosidase from bovine kidney (Sigma, 10 milliunits), β- N -acetylglucosaminidase from jack bean (Sigma, 6.25 milliunits), recombinant Xanthomonas manihotis α1–2/3-mannosidase (New England Biolabs, 6.4 units), and recombinant X. manihotis α1–6-mannosidase (New England Biolabs, 8 units). .. For removal of α1,3-linked fucose, glycan samples were dried in a SpeedVac and then incubated with 3 μl of 48% (v/v) hydrofluoric acid (HF) on ice for 24 h. The HF was allowed to evaporate overnight.

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  • 84
    Millipore protein phosphatase 2a2 from bovine kidney
    Protein Phosphatase 2a2 From Bovine Kidney, supplied by Millipore, used in various techniques. Bioz Stars score: 84/100, based on 2525 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/protein phosphatase 2a2 from bovine kidney/product/Millipore
    Average 84 stars, based on 2525 article reviews
    Price from $9.99 to $1999.99
    protein phosphatase 2a2 from bovine kidney - by Bioz Stars, 2020-09
    84/100 stars
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