bovine dnase i  (Thermo Fisher)


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    Structured Review

    Thermo Fisher bovine dnase i
    Bovine Dnase I, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bovine dnase i/product/Thermo Fisher
    Average 97 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    bovine dnase i - by Bioz Stars, 2020-03
    97/100 stars

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    Related Articles

    Agarose Gel Electrophoresis:

    Article Title: Deoxyribonuclease I Activity, Cell-Free DNA, and Risk of Liver Cancer in a Prospective Cohort
    Article Snippet: Briefly, to determine serum DNase I activity, 4 µL of sera was diluted with 2 µL of 1 X PBS and applied to a 1.0% agarose gel embedded with magnesium chloride (20 mM), calcium chloride (2 mM), and ethidium bromide (0.01 mg/mL)-intercalated calf thymus DNA (0.2 mg/mL) on petri plates. .. DNase I activity was calculated using a standard curve generated from known concentrations of bovine DNase I (Thermo Fisher Scientific, Waltham, MA).

    Synthesized:

    Article Title: Succination of Thiol Groups in Adipose Tissue Proteins in Diabetes
    Article Snippet: 2SC and [U-13 C3 ,15 N]2SC were synthesized as previously described ( ). .. Bovine pancreatic DNase I was obtained from Pierce.

    Isolation:

    Article Title: Genomic imbalance of HMMR/RHAMM regulates the sensitivity and response of malignant peripheral nerve sheath tumour cells to aurora kinase inhibition
    Article Snippet: Array comparative genomic hybridization (a-CGH) Genomic DNA was isolated using the Gentra Puregene Cell Kit (Qiagen). .. Purified DNA was digested with Bovine DNase I (Ambion).

    Labeling:

    Article Title: Uncovering the biology of multiple myeloma among African Americans: a comprehensive genomics approach
    Article Snippet: .. Briefly, 800 nanograms (ng) of DNA obtained from CD138+ selected plasma cells and DNA from a normal female reference (Promega, Madison, WI) were independently digested with Bovine DNAse I (Ambion, Austin, TX) and directly labeled with Cy5 and Cy3, respectively, using the BioPrime Array CGH Genomic Labeling Module (Invitrogen, Carlsbad, CA). .. Labeled DNA was purified using Vivaspin 500 columns (Satorius Stedim Biotech, Goettingen, Germany).

    Article Title: Genomic imbalance of HMMR/RHAMM regulates the sensitivity and response of malignant peripheral nerve sheath tumour cells to aurora kinase inhibition
    Article Snippet: Purified DNA was digested with Bovine DNase I (Ambion). .. Test and control samples were labelled with Alexa5 and Alexa3 dyes respectively using the BioPrime Total Genomic Labeling Kit (Invitrogen).

    Article Title: Integrated Genomic and Epigenomic Analysis of Breast Cancer Brain Metastasis
    Article Snippet: .. Briefly, 800 ng of experimental and normal female reference (Promega, Madison, WI) gDNA were independently digested with Bovine DNAse I (Ambion) and directly labeled with Cy5-dUTP and Cy3-dUTP, respectively, using the BioPrime Array CGH Genomic Labeling Module (Invitrogen, Carlsbad, CA). .. Labeled DNA was purified using Vivaspin 500 columns (Satorius Stedim Biotech, Goettingen, Germany).

    Article Title: Genome-Wide Characterization of Pancreatic Adenocarcinoma Patients Using Next Generation Sequencing
    Article Snippet: The digestion, labeling, and hybridization steps were performed as previously described with minor modifications . .. Briefly, 1.2 ug of tumor and reference DNA were independently digested with Bovine DNase I (Ambion, Austin, TX) for 12 minutes at room temperature.

    Article Title: Deep sequencing identifies genetic heterogeneity and recurrent convergent evolution in chronic lymphocytic leukemia
    Article Snippet: One microgram of tumor and reference DNA were independently fragmented with bovine DNase I (Ambion) for 12 minutes at room temperature. .. Tumor and reference samples were labeled with Alexa Fluor 5 and Alexa Fluor 3 dyes, respectively.

    Article Title: Copy number and targeted mutational analysis reveals novel somatic events in metastatic prostate tumors
    Article Snippet: .. Briefly, 1 μg of sample genomic DNA and normal female reference (Promega) were digested with Bovine DNase I and directly labeled with either Cy5 or Cy3 using the BioPrime Array CGH Genomic Labeling Module (Invitrogen). .. Labeled DNA was purified using Vivaspin 500 spin columns (Sartorius).

    Purification:

    Article Title: Uncovering the biology of multiple myeloma among African Americans: a comprehensive genomics approach
    Article Snippet: Briefly, 800 nanograms (ng) of DNA obtained from CD138+ selected plasma cells and DNA from a normal female reference (Promega, Madison, WI) were independently digested with Bovine DNAse I (Ambion, Austin, TX) and directly labeled with Cy5 and Cy3, respectively, using the BioPrime Array CGH Genomic Labeling Module (Invitrogen, Carlsbad, CA). .. Labeled DNA was purified using Vivaspin 500 columns (Satorius Stedim Biotech, Goettingen, Germany).

    Article Title: Genomic imbalance of HMMR/RHAMM regulates the sensitivity and response of malignant peripheral nerve sheath tumour cells to aurora kinase inhibition
    Article Snippet: .. Purified DNA was digested with Bovine DNase I (Ambion). .. Test and control samples were labelled with Alexa5 and Alexa3 dyes respectively using the BioPrime Total Genomic Labeling Kit (Invitrogen).

    Article Title: Integrated Genomic and Epigenomic Analysis of Breast Cancer Brain Metastasis
    Article Snippet: Briefly, 800 ng of experimental and normal female reference (Promega, Madison, WI) gDNA were independently digested with Bovine DNAse I (Ambion) and directly labeled with Cy5-dUTP and Cy3-dUTP, respectively, using the BioPrime Array CGH Genomic Labeling Module (Invitrogen, Carlsbad, CA). .. Labeled DNA was purified using Vivaspin 500 columns (Satorius Stedim Biotech, Goettingen, Germany).

    Article Title: Copy number and targeted mutational analysis reveals novel somatic events in metastatic prostate tumors
    Article Snippet: Briefly, 1 μg of sample genomic DNA and normal female reference (Promega) were digested with Bovine DNase I and directly labeled with either Cy5 or Cy3 using the BioPrime Array CGH Genomic Labeling Module (Invitrogen). .. Labeled DNA was purified using Vivaspin 500 spin columns (Sartorius).

    Microarray:

    Article Title: Uncovering the biology of multiple myeloma among African Americans: a comprehensive genomics approach
    Article Snippet: Briefly, 800 nanograms (ng) of DNA obtained from CD138+ selected plasma cells and DNA from a normal female reference (Promega, Madison, WI) were independently digested with Bovine DNAse I (Ambion, Austin, TX) and directly labeled with Cy5 and Cy3, respectively, using the BioPrime Array CGH Genomic Labeling Module (Invitrogen, Carlsbad, CA). .. Equal amounts of labeled, purified tumor and reference DNA were hybridized to the microarray in a rotary oven at 65°C for 40 hours at a rotation speed of 20 rpm.

    Article Title: Genomic imbalance of HMMR/RHAMM regulates the sensitivity and response of malignant peripheral nerve sheath tumour cells to aurora kinase inhibition
    Article Snippet: Purified DNA was digested with Bovine DNase I (Ambion). .. Copy number estimates were extracted from the microarray image files using Feature Extraction 10.5 (Agilent) and the data were analyzed in Agilent Genomic Workbench 6.5 (Agilent) after centralization and fuzzy zero normalization matrixes were applied.

    Article Title: Integrated Genomic and Epigenomic Analysis of Breast Cancer Brain Metastasis
    Article Snippet: Copy Number Analysis Array-based comparative genomic hybridization (aCGH) was performed on 19 BBM samples using the Agilent SurePrint G3 Human CGH Microarray Kit, 1×1M, which have an average probe spacing of 2.1 Kb (Agilent Technologies). .. Briefly, 800 ng of experimental and normal female reference (Promega, Madison, WI) gDNA were independently digested with Bovine DNAse I (Ambion) and directly labeled with Cy5-dUTP and Cy3-dUTP, respectively, using the BioPrime Array CGH Genomic Labeling Module (Invitrogen, Carlsbad, CA).

    Article Title: Genome-Wide Characterization of Pancreatic Adenocarcinoma Patients Using Next Generation Sequencing
    Article Snippet: Array CGH (aCGH) for patient 1 Samples were run with the SurePrint G3 Human aCGH Microarray 1 M (Agilent Technologies, Palo Alto, CA). .. Briefly, 1.2 ug of tumor and reference DNA were independently digested with Bovine DNase I (Ambion, Austin, TX) for 12 minutes at room temperature.

    Article Title: Deep sequencing identifies genetic heterogeneity and recurrent convergent evolution in chronic lymphocytic leukemia
    Article Snippet: CNAs were analyzed using aCGH as previously published., , Briefly, aCGH was performed using the SurePrint G3 Human Microarray Kit (Agilent Technologies). .. One microgram of tumor and reference DNA were independently fragmented with bovine DNase I (Ambion) for 12 minutes at room temperature.

    Article Title: Copy number and targeted mutational analysis reveals novel somatic events in metastatic prostate tumors
    Article Snippet: Array CGH was performed on both the Human Genome CGH 244K array and the 1M Human Genome CGH microarray platform for our samples (Agilent Technologies). .. Briefly, 1 μg of sample genomic DNA and normal female reference (Promega) were digested with Bovine DNase I and directly labeled with either Cy5 or Cy3 using the BioPrime Array CGH Genomic Labeling Module (Invitrogen).

    Generated:

    Article Title: Deoxyribonuclease I Activity, Cell-Free DNA, and Risk of Liver Cancer in a Prospective Cohort
    Article Snippet: .. DNase I activity was calculated using a standard curve generated from known concentrations of bovine DNase I (Thermo Fisher Scientific, Waltham, MA). .. The within-batch coefficient of variation (CV) for DNase I activity measured in units per milliliter was 20.2 and measured as radius in millimeters was 9.03.

    Diffusion-based Assay:

    Article Title: Deoxyribonuclease I Activity, Cell-Free DNA, and Risk of Liver Cancer in a Prospective Cohort
    Article Snippet: Serum DNase I activity was measured by Single Radial Enzyme Diffusion (SRED). .. DNase I activity was calculated using a standard curve generated from known concentrations of bovine DNase I (Thermo Fisher Scientific, Waltham, MA).

    Activity Assay:

    Article Title: Deoxyribonuclease I Activity, Cell-Free DNA, and Risk of Liver Cancer in a Prospective Cohort
    Article Snippet: .. DNase I activity was calculated using a standard curve generated from known concentrations of bovine DNase I (Thermo Fisher Scientific, Waltham, MA). .. The within-batch coefficient of variation (CV) for DNase I activity measured in units per milliliter was 20.2 and measured as radius in millimeters was 9.03.

    Cell Culture:

    Article Title: Succination of Thiol Groups in Adipose Tissue Proteins in Diabetes
    Article Snippet: Bovine pancreatic DNase I was obtained from Pierce. .. 3T3-L1 murine fibroblasts were obtained from the American Type Culture Collection (Manassas, VA) and cultured as described previously ( ).

    Staining:

    Article Title: Succination of Thiol Groups in Adipose Tissue Proteins in Diabetes
    Article Snippet: Sypro Ruby gel stain was purchased from Molecular Probes, Inc. (Eugene, OR). .. Bovine pancreatic DNase I was obtained from Pierce.

    Software:

    Article Title: Genome-Wide Characterization of Pancreatic Adenocarcinoma Patients Using Next Generation Sequencing
    Article Snippet: Briefly, 1.2 ug of tumor and reference DNA were independently digested with Bovine DNase I (Ambion, Austin, TX) for 12 minutes at room temperature. .. Microarrays were scanned and features were extracted with Feature Extraction software (Agilent Technologies).

    Article Title: Deep sequencing identifies genetic heterogeneity and recurrent convergent evolution in chronic lymphocytic leukemia
    Article Snippet: One microgram of tumor and reference DNA were independently fragmented with bovine DNase I (Ambion) for 12 minutes at room temperature. .. Microarrays were scanned in a DNA Microarray Scanner, and features were extracted with Feature Extraction software (Agilent Technologies).

    Hybridization:

    Article Title: Uncovering the biology of multiple myeloma among African Americans: a comprehensive genomics approach
    Article Snippet: Paragraph title: Array-based comparative genomic hybridization ... Briefly, 800 nanograms (ng) of DNA obtained from CD138+ selected plasma cells and DNA from a normal female reference (Promega, Madison, WI) were independently digested with Bovine DNAse I (Ambion, Austin, TX) and directly labeled with Cy5 and Cy3, respectively, using the BioPrime Array CGH Genomic Labeling Module (Invitrogen, Carlsbad, CA).

    Article Title: Genomic imbalance of HMMR/RHAMM regulates the sensitivity and response of malignant peripheral nerve sheath tumour cells to aurora kinase inhibition
    Article Snippet: Paragraph title: Array comparative genomic hybridization (a-CGH) ... Purified DNA was digested with Bovine DNase I (Ambion).

    Article Title: Integrated Genomic and Epigenomic Analysis of Breast Cancer Brain Metastasis
    Article Snippet: Copy Number Analysis Array-based comparative genomic hybridization (aCGH) was performed on 19 BBM samples using the Agilent SurePrint G3 Human CGH Microarray Kit, 1×1M, which have an average probe spacing of 2.1 Kb (Agilent Technologies). .. Briefly, 800 ng of experimental and normal female reference (Promega, Madison, WI) gDNA were independently digested with Bovine DNAse I (Ambion) and directly labeled with Cy5-dUTP and Cy3-dUTP, respectively, using the BioPrime Array CGH Genomic Labeling Module (Invitrogen, Carlsbad, CA).

    Article Title: Genome-Wide Characterization of Pancreatic Adenocarcinoma Patients Using Next Generation Sequencing
    Article Snippet: The digestion, labeling, and hybridization steps were performed as previously described with minor modifications . .. Briefly, 1.2 ug of tumor and reference DNA were independently digested with Bovine DNase I (Ambion, Austin, TX) for 12 minutes at room temperature.

    Article Title: Deep sequencing identifies genetic heterogeneity and recurrent convergent evolution in chronic lymphocytic leukemia
    Article Snippet: One microgram of tumor and reference DNA were independently fragmented with bovine DNase I (Ambion) for 12 minutes at room temperature. .. DNA samples from a pool of 9 female lymphoblastoid cell lines from the Coriell biorepository were used as the normal reference in the hybridization experiments.

    Article Title: Copy number and targeted mutational analysis reveals novel somatic events in metastatic prostate tumors
    Article Snippet: Paragraph title: Array-based comparative genomic hybridization ... Briefly, 1 μg of sample genomic DNA and normal female reference (Promega) were digested with Bovine DNase I and directly labeled with either Cy5 or Cy3 using the BioPrime Array CGH Genomic Labeling Module (Invitrogen).

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  • News
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  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 97
    Thermo Fisher bovine dnase i
    Bovine Dnase I, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bovine dnase i/product/Thermo Fisher
    Average 97 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    bovine dnase i - by Bioz Stars, 2020-03
    97/100 stars
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