Structured Review

Axygen boil proof microcentrifuge tube
Boil Proof Microcentrifuge Tube, supplied by Axygen, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/boil proof microcentrifuge tube/product/Axygen
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
boil proof microcentrifuge tube - by Bioz Stars, 2021-06
86/100 stars

Images

Related Articles

Polymerase Chain Reaction:

Article Title: Genome-scale CRISPR-Cas9 Knockout and Transcriptional Activation Screening
Article Snippet: Quick-gDNA MidiPrep (Zymo Research, cat. no. D3100) DNA Binding Buffer (Zymo Research, cat. no. D4004-1-L) DNA Wash Buffer (Zymo Research, cat. no. D4003-2-24) DNA Elution Buffer (Zymo Research, cat. no. D3004-4-4) Ethyl alcohol, Pure (Sigma-Aldrich, cat. no. 459844-500ML) Primers for cloning the validation sgRNAs are listed in . (Integrated DNA technologies) .. Axygen 8-Strip PCR tubes (Fisher Scientific, cat. no. 14-222-250) Axygen PCR plates, 96 well (VWR, cat. no. PCR-96M2-HS-C) 384-well optical plate (e.g. Roche, LightCycler 480 Multiwell plate 384, cat. no. 5102430001) Axygen 1.5 ml Boil-Proof Microcentrifuge Tubes, (VWR, cat. no. 10011-702) Falcon tubes, polypropylene, 15 ml (Corning cat. no. 352097) Falcon tubes, polypropylene, 50 ml (Corning, cat. no. 352070) Filtered sterile pipette tips (e.g. Rainin) 100 mm × 15 mm Not TC-Treated Bacteriological Petri Dish (Corning, cat. no. 351029) 245mm Square BioAssay Dish without Handles, not TC-Treated Culture (Corning, cat. no. 431111) VWR Bacti Cell Spreaders (VWR, cat. no. 60828-688) AirPore Tape Sheets (Qiagen, cat. no. 19571) Nunc EasYFlask 25cm2 , Filter Cap, 7 ml working volume (T25 flask; Thermo Scientific, cat. no. 156367) Nunc EasYFlask 75cm2 , Filter Cap, 25 ml working volume, (T75 flask; Thermo Scientific, cat. no. 156499) Nunc EasYFlask 225 cm2 , filter cap, 70 ml working volume (T225 flask; Thermo Scientific, cat. no. 159934) Corning bottle-top vacuum filter system, 0.22 μM (Sigma-Aldrich, cat. no. CLS431098) Stericup filter unit, 0.45 μM (Millipore, cat. no. SCHVU02RE) Syringe filter unit, 0.45 μM (Millipore, cat. no. SLHV013SL) Disposable Syringes with Luer-Lok Tip (Fisher Scientific, cat. no. 14-829-45) Falcon tissue culture plate, 6 wells (Corning, cat. no. 353224) Falcon tissue culture plate, 12 wells (Corning, cat. no. 353043) Falcon tissue culture dish, 100mm (Corning, cat. no. 353003) 96 well flat clear bottom black polystyrene TC-treated microplates (Corning, cat. no. 3904) BD BioCoat clear Poly-D-Lysine 96 well clear flat bottom TC-treated microplate (Corning, cat. no. 356461) Cellometer SD100 Counting Chambers (Nexcelom Bioscience, cat. no. CHT4-SD100-002) Zymo-Spin V with Reservoir (Zymo Research, cat. no. C1016-25) Collection Tubes, 2 ml (Zymo Research, cat. no. C1001-25) Amicon Ultra-15 Centrifugal Filter Unit with Ultracel-100 membrane (Millipore, cat. no. UFC910008) Thermocycler with programmable temperature stepping functionality, 96 well (e.g. .. Applied Biosystems Veriti, cat. no. 4375786) Real-time PCR system, 384 well (e.g. Roche Lightcycler 480 cat. no. 05015243001) Desktop microcentrifuges (e.g. Eppendorf, cat. nos.

Article Title: SHERLOCK: Nucleic acid detection with CRISPR nucleases
Article Snippet: Novex™ TBE-Urea Gels, 10% (ThermoFisher Scientific cat. no. EC68755BOX) SYBR™ Gold Nucleic Acid Gel Stain 10,000X Concentrate in DMSO (ThermoFisher Scientific cat. no. S11494) TwistAmp® Basic (TwistDX cat. no. TABAS03KIT) TwistAmp® Basic RT (TwistDX cat. no. TABARTS01kit) RNase Inhibitor, Murine (New England Biolabs cat. no. M0314S) NxGen® T7 RNA Polymerase 125000U (Lucigen cat. No. 30223–2 30223–2) RNaseAlert™ Lab Test Kit v2 (Invitrogen cat. No. 4479768) Ribonucleotide Solution Mix (New England Biolabs cat. No. N0466L) Milenia HybriDetect 1 (TwistDx™ cat. no. MILENIA01) .. Constant temperature incubator (e.g., Quincy Lab, Inc Incubator Model 12–140E) 100 mm × 15 mm Not TC-Treated Bacteriological Petri Dish (Corning, cat. no. 351029) Culture flasks, 1 L (e.g., Corning® 5100–1L PYREX® Wide Mouth flask) Biological shaker (e.g., Eppendorf Innnova S44i) Fisherbrand™ Disposable Cuvettes; Polystyrene (cat. no. 14–955-127) Floor centrifuge with 4 L capacity (e.g., Beckman-Coulter, Avanti J-E) Gel electrophoresis tank and power source (e.g., Invitrogen™ Mini Gel Tank; VWR Power Source 250 V cat. no. 93000–746) LM20 Microfluidizer® from Microfluidics Corning™ Polypropylene Centrifuge Tubes, 250 mL (cat. no. 05–538-53) Tube rotator (e.g., Scilogex MX-RL-Pro, cat. no. 824222019999) Plate shaker (e.g., VWR® Mini Shaker) Chromatography column (e.g., Bio-Rad Glass Econo-Column®) eStain® L1 Protein Staining System (GenScript®) or Coomassie Stain (Coomassie Brilliant Blue R-250, cat. no. 1610400, Bio-Rad) and destain solution (40% methanol, 10% acetic acid) FPLC (e.g., ÄKTA pure by GE Healthcare Life Sciences) LightCycler® 480 Instrument II (Roche Diagnostics, product no. 05015243001) QX200™ droplet generator (Bio-Rad, cat. no. 186–4002) QX200™ droplet reader (Bio-Rad, cat. no. 186–4003) Benchtop centrifuge (e.g., Beckman Coulter Allegra X-15) Axygen™ 8-Strip PCR tubes (Fisher Scientific, cat. no. 14–222-250) Axygen™ PCR plates, 96 well (VWR, cat. no. PCR-96M2-HS-C) 384-well optical plate (e.g., Roche, LightCycler 480 Multiwell plate 384, cat. no. 5102430001) Axygen 1.5 mL Boil-Proof Microcentrifuge Tubes, (VWR, cat. no. 10011–702) Falcon tubes, polypropylene, 15 mL (Corning cat. no. 352097) Falcon tubes, polypropylene, 50 mL (Corning, cat. no. 352070) Eppendorf Safe-lock microcentrifuge tubes, 1.5 mL (Eppendorf, cat. no. T9661–1000EA) Filtered sterile pipette tips (e.g., Rainin) Corning bottle-top vacuum filter system, 0.22 μM (Sigma-Aldrich, cat. no. CLS431098) Stericup filter unit, 0.45 μM (Millipore, cat. no. SCHVU02RE) Amicon Ultra-15 Centrifugal Filter Unit with Ultracel-50 membrane (Sigma-Aldrich, cat. no. UFC905024) Thermocycler with programmable temperature stepping functionality, 96 well (e.g., Applied Biosystems Veriti, cat. no. 4375786) Desktop microcentrifuges (e.g., Eppendorf, cat. no. 5424) Digital gel imaging system (GelDoc EZ, Bio-Rad, cat. no. 170–8270) and white sample tray (Bio-Rad, cat. no. 170–8272) ) UV spectrophotometer (e.g., NanoDrop 2000c, Thermo Scientific) Plate spectrophotometer (e.g., Synergy H4 Hybrid Multi-Mode Microplate Reader, BioTek) .. Reconstitute the LB Broth with agar at a concentration of 35 g L−1 in deionized water and swirl to mix.

Transferring:

Article Title: Genome-scale CRISPR-Cas9 Knockout and Transcriptional Activation Screening
Article Snippet: Quick-gDNA MidiPrep (Zymo Research, cat. no. D3100) DNA Binding Buffer (Zymo Research, cat. no. D4004-1-L) DNA Wash Buffer (Zymo Research, cat. no. D4003-2-24) DNA Elution Buffer (Zymo Research, cat. no. D3004-4-4) Ethyl alcohol, Pure (Sigma-Aldrich, cat. no. 459844-500ML) Primers for cloning the validation sgRNAs are listed in . (Integrated DNA technologies) .. Axygen 8-Strip PCR tubes (Fisher Scientific, cat. no. 14-222-250) Axygen PCR plates, 96 well (VWR, cat. no. PCR-96M2-HS-C) 384-well optical plate (e.g. Roche, LightCycler 480 Multiwell plate 384, cat. no. 5102430001) Axygen 1.5 ml Boil-Proof Microcentrifuge Tubes, (VWR, cat. no. 10011-702) Falcon tubes, polypropylene, 15 ml (Corning cat. no. 352097) Falcon tubes, polypropylene, 50 ml (Corning, cat. no. 352070) Filtered sterile pipette tips (e.g. Rainin) 100 mm × 15 mm Not TC-Treated Bacteriological Petri Dish (Corning, cat. no. 351029) 245mm Square BioAssay Dish without Handles, not TC-Treated Culture (Corning, cat. no. 431111) VWR Bacti Cell Spreaders (VWR, cat. no. 60828-688) AirPore Tape Sheets (Qiagen, cat. no. 19571) Nunc EasYFlask 25cm2 , Filter Cap, 7 ml working volume (T25 flask; Thermo Scientific, cat. no. 156367) Nunc EasYFlask 75cm2 , Filter Cap, 25 ml working volume, (T75 flask; Thermo Scientific, cat. no. 156499) Nunc EasYFlask 225 cm2 , filter cap, 70 ml working volume (T225 flask; Thermo Scientific, cat. no. 159934) Corning bottle-top vacuum filter system, 0.22 μM (Sigma-Aldrich, cat. no. CLS431098) Stericup filter unit, 0.45 μM (Millipore, cat. no. SCHVU02RE) Syringe filter unit, 0.45 μM (Millipore, cat. no. SLHV013SL) Disposable Syringes with Luer-Lok Tip (Fisher Scientific, cat. no. 14-829-45) Falcon tissue culture plate, 6 wells (Corning, cat. no. 353224) Falcon tissue culture plate, 12 wells (Corning, cat. no. 353043) Falcon tissue culture dish, 100mm (Corning, cat. no. 353003) 96 well flat clear bottom black polystyrene TC-treated microplates (Corning, cat. no. 3904) BD BioCoat clear Poly-D-Lysine 96 well clear flat bottom TC-treated microplate (Corning, cat. no. 356461) Cellometer SD100 Counting Chambers (Nexcelom Bioscience, cat. no. CHT4-SD100-002) Zymo-Spin V with Reservoir (Zymo Research, cat. no. C1016-25) Collection Tubes, 2 ml (Zymo Research, cat. no. C1001-25) Amicon Ultra-15 Centrifugal Filter Unit with Ultracel-100 membrane (Millipore, cat. no. UFC910008) Thermocycler with programmable temperature stepping functionality, 96 well (e.g. .. Applied Biosystems Veriti, cat. no. 4375786) Real-time PCR system, 384 well (e.g. Roche Lightcycler 480 cat. no. 05015243001) Desktop microcentrifuges (e.g. Eppendorf, cat. nos.

Article Title: SHERLOCK: Nucleic acid detection with CRISPR nucleases
Article Snippet: Novex™ TBE-Urea Gels, 10% (ThermoFisher Scientific cat. no. EC68755BOX) SYBR™ Gold Nucleic Acid Gel Stain 10,000X Concentrate in DMSO (ThermoFisher Scientific cat. no. S11494) TwistAmp® Basic (TwistDX cat. no. TABAS03KIT) TwistAmp® Basic RT (TwistDX cat. no. TABARTS01kit) RNase Inhibitor, Murine (New England Biolabs cat. no. M0314S) NxGen® T7 RNA Polymerase 125000U (Lucigen cat. No. 30223–2 30223–2) RNaseAlert™ Lab Test Kit v2 (Invitrogen cat. No. 4479768) Ribonucleotide Solution Mix (New England Biolabs cat. No. N0466L) Milenia HybriDetect 1 (TwistDx™ cat. no. MILENIA01) .. Constant temperature incubator (e.g., Quincy Lab, Inc Incubator Model 12–140E) 100 mm × 15 mm Not TC-Treated Bacteriological Petri Dish (Corning, cat. no. 351029) Culture flasks, 1 L (e.g., Corning® 5100–1L PYREX® Wide Mouth flask) Biological shaker (e.g., Eppendorf Innnova S44i) Fisherbrand™ Disposable Cuvettes; Polystyrene (cat. no. 14–955-127) Floor centrifuge with 4 L capacity (e.g., Beckman-Coulter, Avanti J-E) Gel electrophoresis tank and power source (e.g., Invitrogen™ Mini Gel Tank; VWR Power Source 250 V cat. no. 93000–746) LM20 Microfluidizer® from Microfluidics Corning™ Polypropylene Centrifuge Tubes, 250 mL (cat. no. 05–538-53) Tube rotator (e.g., Scilogex MX-RL-Pro, cat. no. 824222019999) Plate shaker (e.g., VWR® Mini Shaker) Chromatography column (e.g., Bio-Rad Glass Econo-Column®) eStain® L1 Protein Staining System (GenScript®) or Coomassie Stain (Coomassie Brilliant Blue R-250, cat. no. 1610400, Bio-Rad) and destain solution (40% methanol, 10% acetic acid) FPLC (e.g., ÄKTA pure by GE Healthcare Life Sciences) LightCycler® 480 Instrument II (Roche Diagnostics, product no. 05015243001) QX200™ droplet generator (Bio-Rad, cat. no. 186–4002) QX200™ droplet reader (Bio-Rad, cat. no. 186–4003) Benchtop centrifuge (e.g., Beckman Coulter Allegra X-15) Axygen™ 8-Strip PCR tubes (Fisher Scientific, cat. no. 14–222-250) Axygen™ PCR plates, 96 well (VWR, cat. no. PCR-96M2-HS-C) 384-well optical plate (e.g., Roche, LightCycler 480 Multiwell plate 384, cat. no. 5102430001) Axygen 1.5 mL Boil-Proof Microcentrifuge Tubes, (VWR, cat. no. 10011–702) Falcon tubes, polypropylene, 15 mL (Corning cat. no. 352097) Falcon tubes, polypropylene, 50 mL (Corning, cat. no. 352070) Eppendorf Safe-lock microcentrifuge tubes, 1.5 mL (Eppendorf, cat. no. T9661–1000EA) Filtered sterile pipette tips (e.g., Rainin) Corning bottle-top vacuum filter system, 0.22 μM (Sigma-Aldrich, cat. no. CLS431098) Stericup filter unit, 0.45 μM (Millipore, cat. no. SCHVU02RE) Amicon Ultra-15 Centrifugal Filter Unit with Ultracel-50 membrane (Sigma-Aldrich, cat. no. UFC905024) Thermocycler with programmable temperature stepping functionality, 96 well (e.g., Applied Biosystems Veriti, cat. no. 4375786) Desktop microcentrifuges (e.g., Eppendorf, cat. no. 5424) Digital gel imaging system (GelDoc EZ, Bio-Rad, cat. no. 170–8270) and white sample tray (Bio-Rad, cat. no. 170–8272) ) UV spectrophotometer (e.g., NanoDrop 2000c, Thermo Scientific) Plate spectrophotometer (e.g., Synergy H4 Hybrid Multi-Mode Microplate Reader, BioTek) .. Reconstitute the LB Broth with agar at a concentration of 35 g L−1 in deionized water and swirl to mix.

Nucleic Acid Electrophoresis:

Article Title: SHERLOCK: Nucleic acid detection with CRISPR nucleases
Article Snippet: Novex™ TBE-Urea Gels, 10% (ThermoFisher Scientific cat. no. EC68755BOX) SYBR™ Gold Nucleic Acid Gel Stain 10,000X Concentrate in DMSO (ThermoFisher Scientific cat. no. S11494) TwistAmp® Basic (TwistDX cat. no. TABAS03KIT) TwistAmp® Basic RT (TwistDX cat. no. TABARTS01kit) RNase Inhibitor, Murine (New England Biolabs cat. no. M0314S) NxGen® T7 RNA Polymerase 125000U (Lucigen cat. No. 30223–2 30223–2) RNaseAlert™ Lab Test Kit v2 (Invitrogen cat. No. 4479768) Ribonucleotide Solution Mix (New England Biolabs cat. No. N0466L) Milenia HybriDetect 1 (TwistDx™ cat. no. MILENIA01) .. Constant temperature incubator (e.g., Quincy Lab, Inc Incubator Model 12–140E) 100 mm × 15 mm Not TC-Treated Bacteriological Petri Dish (Corning, cat. no. 351029) Culture flasks, 1 L (e.g., Corning® 5100–1L PYREX® Wide Mouth flask) Biological shaker (e.g., Eppendorf Innnova S44i) Fisherbrand™ Disposable Cuvettes; Polystyrene (cat. no. 14–955-127) Floor centrifuge with 4 L capacity (e.g., Beckman-Coulter, Avanti J-E) Gel electrophoresis tank and power source (e.g., Invitrogen™ Mini Gel Tank; VWR Power Source 250 V cat. no. 93000–746) LM20 Microfluidizer® from Microfluidics Corning™ Polypropylene Centrifuge Tubes, 250 mL (cat. no. 05–538-53) Tube rotator (e.g., Scilogex MX-RL-Pro, cat. no. 824222019999) Plate shaker (e.g., VWR® Mini Shaker) Chromatography column (e.g., Bio-Rad Glass Econo-Column®) eStain® L1 Protein Staining System (GenScript®) or Coomassie Stain (Coomassie Brilliant Blue R-250, cat. no. 1610400, Bio-Rad) and destain solution (40% methanol, 10% acetic acid) FPLC (e.g., ÄKTA pure by GE Healthcare Life Sciences) LightCycler® 480 Instrument II (Roche Diagnostics, product no. 05015243001) QX200™ droplet generator (Bio-Rad, cat. no. 186–4002) QX200™ droplet reader (Bio-Rad, cat. no. 186–4003) Benchtop centrifuge (e.g., Beckman Coulter Allegra X-15) Axygen™ 8-Strip PCR tubes (Fisher Scientific, cat. no. 14–222-250) Axygen™ PCR plates, 96 well (VWR, cat. no. PCR-96M2-HS-C) 384-well optical plate (e.g., Roche, LightCycler 480 Multiwell plate 384, cat. no. 5102430001) Axygen 1.5 mL Boil-Proof Microcentrifuge Tubes, (VWR, cat. no. 10011–702) Falcon tubes, polypropylene, 15 mL (Corning cat. no. 352097) Falcon tubes, polypropylene, 50 mL (Corning, cat. no. 352070) Eppendorf Safe-lock microcentrifuge tubes, 1.5 mL (Eppendorf, cat. no. T9661–1000EA) Filtered sterile pipette tips (e.g., Rainin) Corning bottle-top vacuum filter system, 0.22 μM (Sigma-Aldrich, cat. no. CLS431098) Stericup filter unit, 0.45 μM (Millipore, cat. no. SCHVU02RE) Amicon Ultra-15 Centrifugal Filter Unit with Ultracel-50 membrane (Sigma-Aldrich, cat. no. UFC905024) Thermocycler with programmable temperature stepping functionality, 96 well (e.g., Applied Biosystems Veriti, cat. no. 4375786) Desktop microcentrifuges (e.g., Eppendorf, cat. no. 5424) Digital gel imaging system (GelDoc EZ, Bio-Rad, cat. no. 170–8270) and white sample tray (Bio-Rad, cat. no. 170–8272) ) UV spectrophotometer (e.g., NanoDrop 2000c, Thermo Scientific) Plate spectrophotometer (e.g., Synergy H4 Hybrid Multi-Mode Microplate Reader, BioTek) .. Reconstitute the LB Broth with agar at a concentration of 35 g L−1 in deionized water and swirl to mix.

Chromatography:

Article Title: SHERLOCK: Nucleic acid detection with CRISPR nucleases
Article Snippet: Novex™ TBE-Urea Gels, 10% (ThermoFisher Scientific cat. no. EC68755BOX) SYBR™ Gold Nucleic Acid Gel Stain 10,000X Concentrate in DMSO (ThermoFisher Scientific cat. no. S11494) TwistAmp® Basic (TwistDX cat. no. TABAS03KIT) TwistAmp® Basic RT (TwistDX cat. no. TABARTS01kit) RNase Inhibitor, Murine (New England Biolabs cat. no. M0314S) NxGen® T7 RNA Polymerase 125000U (Lucigen cat. No. 30223–2 30223–2) RNaseAlert™ Lab Test Kit v2 (Invitrogen cat. No. 4479768) Ribonucleotide Solution Mix (New England Biolabs cat. No. N0466L) Milenia HybriDetect 1 (TwistDx™ cat. no. MILENIA01) .. Constant temperature incubator (e.g., Quincy Lab, Inc Incubator Model 12–140E) 100 mm × 15 mm Not TC-Treated Bacteriological Petri Dish (Corning, cat. no. 351029) Culture flasks, 1 L (e.g., Corning® 5100–1L PYREX® Wide Mouth flask) Biological shaker (e.g., Eppendorf Innnova S44i) Fisherbrand™ Disposable Cuvettes; Polystyrene (cat. no. 14–955-127) Floor centrifuge with 4 L capacity (e.g., Beckman-Coulter, Avanti J-E) Gel electrophoresis tank and power source (e.g., Invitrogen™ Mini Gel Tank; VWR Power Source 250 V cat. no. 93000–746) LM20 Microfluidizer® from Microfluidics Corning™ Polypropylene Centrifuge Tubes, 250 mL (cat. no. 05–538-53) Tube rotator (e.g., Scilogex MX-RL-Pro, cat. no. 824222019999) Plate shaker (e.g., VWR® Mini Shaker) Chromatography column (e.g., Bio-Rad Glass Econo-Column®) eStain® L1 Protein Staining System (GenScript®) or Coomassie Stain (Coomassie Brilliant Blue R-250, cat. no. 1610400, Bio-Rad) and destain solution (40% methanol, 10% acetic acid) FPLC (e.g., ÄKTA pure by GE Healthcare Life Sciences) LightCycler® 480 Instrument II (Roche Diagnostics, product no. 05015243001) QX200™ droplet generator (Bio-Rad, cat. no. 186–4002) QX200™ droplet reader (Bio-Rad, cat. no. 186–4003) Benchtop centrifuge (e.g., Beckman Coulter Allegra X-15) Axygen™ 8-Strip PCR tubes (Fisher Scientific, cat. no. 14–222-250) Axygen™ PCR plates, 96 well (VWR, cat. no. PCR-96M2-HS-C) 384-well optical plate (e.g., Roche, LightCycler 480 Multiwell plate 384, cat. no. 5102430001) Axygen 1.5 mL Boil-Proof Microcentrifuge Tubes, (VWR, cat. no. 10011–702) Falcon tubes, polypropylene, 15 mL (Corning cat. no. 352097) Falcon tubes, polypropylene, 50 mL (Corning, cat. no. 352070) Eppendorf Safe-lock microcentrifuge tubes, 1.5 mL (Eppendorf, cat. no. T9661–1000EA) Filtered sterile pipette tips (e.g., Rainin) Corning bottle-top vacuum filter system, 0.22 μM (Sigma-Aldrich, cat. no. CLS431098) Stericup filter unit, 0.45 μM (Millipore, cat. no. SCHVU02RE) Amicon Ultra-15 Centrifugal Filter Unit with Ultracel-50 membrane (Sigma-Aldrich, cat. no. UFC905024) Thermocycler with programmable temperature stepping functionality, 96 well (e.g., Applied Biosystems Veriti, cat. no. 4375786) Desktop microcentrifuges (e.g., Eppendorf, cat. no. 5424) Digital gel imaging system (GelDoc EZ, Bio-Rad, cat. no. 170–8270) and white sample tray (Bio-Rad, cat. no. 170–8272) ) UV spectrophotometer (e.g., NanoDrop 2000c, Thermo Scientific) Plate spectrophotometer (e.g., Synergy H4 Hybrid Multi-Mode Microplate Reader, BioTek) .. Reconstitute the LB Broth with agar at a concentration of 35 g L−1 in deionized water and swirl to mix.

Staining:

Article Title: SHERLOCK: Nucleic acid detection with CRISPR nucleases
Article Snippet: Novex™ TBE-Urea Gels, 10% (ThermoFisher Scientific cat. no. EC68755BOX) SYBR™ Gold Nucleic Acid Gel Stain 10,000X Concentrate in DMSO (ThermoFisher Scientific cat. no. S11494) TwistAmp® Basic (TwistDX cat. no. TABAS03KIT) TwistAmp® Basic RT (TwistDX cat. no. TABARTS01kit) RNase Inhibitor, Murine (New England Biolabs cat. no. M0314S) NxGen® T7 RNA Polymerase 125000U (Lucigen cat. No. 30223–2 30223–2) RNaseAlert™ Lab Test Kit v2 (Invitrogen cat. No. 4479768) Ribonucleotide Solution Mix (New England Biolabs cat. No. N0466L) Milenia HybriDetect 1 (TwistDx™ cat. no. MILENIA01) .. Constant temperature incubator (e.g., Quincy Lab, Inc Incubator Model 12–140E) 100 mm × 15 mm Not TC-Treated Bacteriological Petri Dish (Corning, cat. no. 351029) Culture flasks, 1 L (e.g., Corning® 5100–1L PYREX® Wide Mouth flask) Biological shaker (e.g., Eppendorf Innnova S44i) Fisherbrand™ Disposable Cuvettes; Polystyrene (cat. no. 14–955-127) Floor centrifuge with 4 L capacity (e.g., Beckman-Coulter, Avanti J-E) Gel electrophoresis tank and power source (e.g., Invitrogen™ Mini Gel Tank; VWR Power Source 250 V cat. no. 93000–746) LM20 Microfluidizer® from Microfluidics Corning™ Polypropylene Centrifuge Tubes, 250 mL (cat. no. 05–538-53) Tube rotator (e.g., Scilogex MX-RL-Pro, cat. no. 824222019999) Plate shaker (e.g., VWR® Mini Shaker) Chromatography column (e.g., Bio-Rad Glass Econo-Column®) eStain® L1 Protein Staining System (GenScript®) or Coomassie Stain (Coomassie Brilliant Blue R-250, cat. no. 1610400, Bio-Rad) and destain solution (40% methanol, 10% acetic acid) FPLC (e.g., ÄKTA pure by GE Healthcare Life Sciences) LightCycler® 480 Instrument II (Roche Diagnostics, product no. 05015243001) QX200™ droplet generator (Bio-Rad, cat. no. 186–4002) QX200™ droplet reader (Bio-Rad, cat. no. 186–4003) Benchtop centrifuge (e.g., Beckman Coulter Allegra X-15) Axygen™ 8-Strip PCR tubes (Fisher Scientific, cat. no. 14–222-250) Axygen™ PCR plates, 96 well (VWR, cat. no. PCR-96M2-HS-C) 384-well optical plate (e.g., Roche, LightCycler 480 Multiwell plate 384, cat. no. 5102430001) Axygen 1.5 mL Boil-Proof Microcentrifuge Tubes, (VWR, cat. no. 10011–702) Falcon tubes, polypropylene, 15 mL (Corning cat. no. 352097) Falcon tubes, polypropylene, 50 mL (Corning, cat. no. 352070) Eppendorf Safe-lock microcentrifuge tubes, 1.5 mL (Eppendorf, cat. no. T9661–1000EA) Filtered sterile pipette tips (e.g., Rainin) Corning bottle-top vacuum filter system, 0.22 μM (Sigma-Aldrich, cat. no. CLS431098) Stericup filter unit, 0.45 μM (Millipore, cat. no. SCHVU02RE) Amicon Ultra-15 Centrifugal Filter Unit with Ultracel-50 membrane (Sigma-Aldrich, cat. no. UFC905024) Thermocycler with programmable temperature stepping functionality, 96 well (e.g., Applied Biosystems Veriti, cat. no. 4375786) Desktop microcentrifuges (e.g., Eppendorf, cat. no. 5424) Digital gel imaging system (GelDoc EZ, Bio-Rad, cat. no. 170–8270) and white sample tray (Bio-Rad, cat. no. 170–8272) ) UV spectrophotometer (e.g., NanoDrop 2000c, Thermo Scientific) Plate spectrophotometer (e.g., Synergy H4 Hybrid Multi-Mode Microplate Reader, BioTek) .. Reconstitute the LB Broth with agar at a concentration of 35 g L−1 in deionized water and swirl to mix.

Fast Protein Liquid Chromatography:

Article Title: SHERLOCK: Nucleic acid detection with CRISPR nucleases
Article Snippet: Novex™ TBE-Urea Gels, 10% (ThermoFisher Scientific cat. no. EC68755BOX) SYBR™ Gold Nucleic Acid Gel Stain 10,000X Concentrate in DMSO (ThermoFisher Scientific cat. no. S11494) TwistAmp® Basic (TwistDX cat. no. TABAS03KIT) TwistAmp® Basic RT (TwistDX cat. no. TABARTS01kit) RNase Inhibitor, Murine (New England Biolabs cat. no. M0314S) NxGen® T7 RNA Polymerase 125000U (Lucigen cat. No. 30223–2 30223–2) RNaseAlert™ Lab Test Kit v2 (Invitrogen cat. No. 4479768) Ribonucleotide Solution Mix (New England Biolabs cat. No. N0466L) Milenia HybriDetect 1 (TwistDx™ cat. no. MILENIA01) .. Constant temperature incubator (e.g., Quincy Lab, Inc Incubator Model 12–140E) 100 mm × 15 mm Not TC-Treated Bacteriological Petri Dish (Corning, cat. no. 351029) Culture flasks, 1 L (e.g., Corning® 5100–1L PYREX® Wide Mouth flask) Biological shaker (e.g., Eppendorf Innnova S44i) Fisherbrand™ Disposable Cuvettes; Polystyrene (cat. no. 14–955-127) Floor centrifuge with 4 L capacity (e.g., Beckman-Coulter, Avanti J-E) Gel electrophoresis tank and power source (e.g., Invitrogen™ Mini Gel Tank; VWR Power Source 250 V cat. no. 93000–746) LM20 Microfluidizer® from Microfluidics Corning™ Polypropylene Centrifuge Tubes, 250 mL (cat. no. 05–538-53) Tube rotator (e.g., Scilogex MX-RL-Pro, cat. no. 824222019999) Plate shaker (e.g., VWR® Mini Shaker) Chromatography column (e.g., Bio-Rad Glass Econo-Column®) eStain® L1 Protein Staining System (GenScript®) or Coomassie Stain (Coomassie Brilliant Blue R-250, cat. no. 1610400, Bio-Rad) and destain solution (40% methanol, 10% acetic acid) FPLC (e.g., ÄKTA pure by GE Healthcare Life Sciences) LightCycler® 480 Instrument II (Roche Diagnostics, product no. 05015243001) QX200™ droplet generator (Bio-Rad, cat. no. 186–4002) QX200™ droplet reader (Bio-Rad, cat. no. 186–4003) Benchtop centrifuge (e.g., Beckman Coulter Allegra X-15) Axygen™ 8-Strip PCR tubes (Fisher Scientific, cat. no. 14–222-250) Axygen™ PCR plates, 96 well (VWR, cat. no. PCR-96M2-HS-C) 384-well optical plate (e.g., Roche, LightCycler 480 Multiwell plate 384, cat. no. 5102430001) Axygen 1.5 mL Boil-Proof Microcentrifuge Tubes, (VWR, cat. no. 10011–702) Falcon tubes, polypropylene, 15 mL (Corning cat. no. 352097) Falcon tubes, polypropylene, 50 mL (Corning, cat. no. 352070) Eppendorf Safe-lock microcentrifuge tubes, 1.5 mL (Eppendorf, cat. no. T9661–1000EA) Filtered sterile pipette tips (e.g., Rainin) Corning bottle-top vacuum filter system, 0.22 μM (Sigma-Aldrich, cat. no. CLS431098) Stericup filter unit, 0.45 μM (Millipore, cat. no. SCHVU02RE) Amicon Ultra-15 Centrifugal Filter Unit with Ultracel-50 membrane (Sigma-Aldrich, cat. no. UFC905024) Thermocycler with programmable temperature stepping functionality, 96 well (e.g., Applied Biosystems Veriti, cat. no. 4375786) Desktop microcentrifuges (e.g., Eppendorf, cat. no. 5424) Digital gel imaging system (GelDoc EZ, Bio-Rad, cat. no. 170–8270) and white sample tray (Bio-Rad, cat. no. 170–8272) ) UV spectrophotometer (e.g., NanoDrop 2000c, Thermo Scientific) Plate spectrophotometer (e.g., Synergy H4 Hybrid Multi-Mode Microplate Reader, BioTek) .. Reconstitute the LB Broth with agar at a concentration of 35 g L−1 in deionized water and swirl to mix.

Imaging:

Article Title: SHERLOCK: Nucleic acid detection with CRISPR nucleases
Article Snippet: Novex™ TBE-Urea Gels, 10% (ThermoFisher Scientific cat. no. EC68755BOX) SYBR™ Gold Nucleic Acid Gel Stain 10,000X Concentrate in DMSO (ThermoFisher Scientific cat. no. S11494) TwistAmp® Basic (TwistDX cat. no. TABAS03KIT) TwistAmp® Basic RT (TwistDX cat. no. TABARTS01kit) RNase Inhibitor, Murine (New England Biolabs cat. no. M0314S) NxGen® T7 RNA Polymerase 125000U (Lucigen cat. No. 30223–2 30223–2) RNaseAlert™ Lab Test Kit v2 (Invitrogen cat. No. 4479768) Ribonucleotide Solution Mix (New England Biolabs cat. No. N0466L) Milenia HybriDetect 1 (TwistDx™ cat. no. MILENIA01) .. Constant temperature incubator (e.g., Quincy Lab, Inc Incubator Model 12–140E) 100 mm × 15 mm Not TC-Treated Bacteriological Petri Dish (Corning, cat. no. 351029) Culture flasks, 1 L (e.g., Corning® 5100–1L PYREX® Wide Mouth flask) Biological shaker (e.g., Eppendorf Innnova S44i) Fisherbrand™ Disposable Cuvettes; Polystyrene (cat. no. 14–955-127) Floor centrifuge with 4 L capacity (e.g., Beckman-Coulter, Avanti J-E) Gel electrophoresis tank and power source (e.g., Invitrogen™ Mini Gel Tank; VWR Power Source 250 V cat. no. 93000–746) LM20 Microfluidizer® from Microfluidics Corning™ Polypropylene Centrifuge Tubes, 250 mL (cat. no. 05–538-53) Tube rotator (e.g., Scilogex MX-RL-Pro, cat. no. 824222019999) Plate shaker (e.g., VWR® Mini Shaker) Chromatography column (e.g., Bio-Rad Glass Econo-Column®) eStain® L1 Protein Staining System (GenScript®) or Coomassie Stain (Coomassie Brilliant Blue R-250, cat. no. 1610400, Bio-Rad) and destain solution (40% methanol, 10% acetic acid) FPLC (e.g., ÄKTA pure by GE Healthcare Life Sciences) LightCycler® 480 Instrument II (Roche Diagnostics, product no. 05015243001) QX200™ droplet generator (Bio-Rad, cat. no. 186–4002) QX200™ droplet reader (Bio-Rad, cat. no. 186–4003) Benchtop centrifuge (e.g., Beckman Coulter Allegra X-15) Axygen™ 8-Strip PCR tubes (Fisher Scientific, cat. no. 14–222-250) Axygen™ PCR plates, 96 well (VWR, cat. no. PCR-96M2-HS-C) 384-well optical plate (e.g., Roche, LightCycler 480 Multiwell plate 384, cat. no. 5102430001) Axygen 1.5 mL Boil-Proof Microcentrifuge Tubes, (VWR, cat. no. 10011–702) Falcon tubes, polypropylene, 15 mL (Corning cat. no. 352097) Falcon tubes, polypropylene, 50 mL (Corning, cat. no. 352070) Eppendorf Safe-lock microcentrifuge tubes, 1.5 mL (Eppendorf, cat. no. T9661–1000EA) Filtered sterile pipette tips (e.g., Rainin) Corning bottle-top vacuum filter system, 0.22 μM (Sigma-Aldrich, cat. no. CLS431098) Stericup filter unit, 0.45 μM (Millipore, cat. no. SCHVU02RE) Amicon Ultra-15 Centrifugal Filter Unit with Ultracel-50 membrane (Sigma-Aldrich, cat. no. UFC905024) Thermocycler with programmable temperature stepping functionality, 96 well (e.g., Applied Biosystems Veriti, cat. no. 4375786) Desktop microcentrifuges (e.g., Eppendorf, cat. no. 5424) Digital gel imaging system (GelDoc EZ, Bio-Rad, cat. no. 170–8270) and white sample tray (Bio-Rad, cat. no. 170–8272) ) UV spectrophotometer (e.g., NanoDrop 2000c, Thermo Scientific) Plate spectrophotometer (e.g., Synergy H4 Hybrid Multi-Mode Microplate Reader, BioTek) .. Reconstitute the LB Broth with agar at a concentration of 35 g L−1 in deionized water and swirl to mix.

Spectrophotometry:

Article Title: SHERLOCK: Nucleic acid detection with CRISPR nucleases
Article Snippet: Novex™ TBE-Urea Gels, 10% (ThermoFisher Scientific cat. no. EC68755BOX) SYBR™ Gold Nucleic Acid Gel Stain 10,000X Concentrate in DMSO (ThermoFisher Scientific cat. no. S11494) TwistAmp® Basic (TwistDX cat. no. TABAS03KIT) TwistAmp® Basic RT (TwistDX cat. no. TABARTS01kit) RNase Inhibitor, Murine (New England Biolabs cat. no. M0314S) NxGen® T7 RNA Polymerase 125000U (Lucigen cat. No. 30223–2 30223–2) RNaseAlert™ Lab Test Kit v2 (Invitrogen cat. No. 4479768) Ribonucleotide Solution Mix (New England Biolabs cat. No. N0466L) Milenia HybriDetect 1 (TwistDx™ cat. no. MILENIA01) .. Constant temperature incubator (e.g., Quincy Lab, Inc Incubator Model 12–140E) 100 mm × 15 mm Not TC-Treated Bacteriological Petri Dish (Corning, cat. no. 351029) Culture flasks, 1 L (e.g., Corning® 5100–1L PYREX® Wide Mouth flask) Biological shaker (e.g., Eppendorf Innnova S44i) Fisherbrand™ Disposable Cuvettes; Polystyrene (cat. no. 14–955-127) Floor centrifuge with 4 L capacity (e.g., Beckman-Coulter, Avanti J-E) Gel electrophoresis tank and power source (e.g., Invitrogen™ Mini Gel Tank; VWR Power Source 250 V cat. no. 93000–746) LM20 Microfluidizer® from Microfluidics Corning™ Polypropylene Centrifuge Tubes, 250 mL (cat. no. 05–538-53) Tube rotator (e.g., Scilogex MX-RL-Pro, cat. no. 824222019999) Plate shaker (e.g., VWR® Mini Shaker) Chromatography column (e.g., Bio-Rad Glass Econo-Column®) eStain® L1 Protein Staining System (GenScript®) or Coomassie Stain (Coomassie Brilliant Blue R-250, cat. no. 1610400, Bio-Rad) and destain solution (40% methanol, 10% acetic acid) FPLC (e.g., ÄKTA pure by GE Healthcare Life Sciences) LightCycler® 480 Instrument II (Roche Diagnostics, product no. 05015243001) QX200™ droplet generator (Bio-Rad, cat. no. 186–4002) QX200™ droplet reader (Bio-Rad, cat. no. 186–4003) Benchtop centrifuge (e.g., Beckman Coulter Allegra X-15) Axygen™ 8-Strip PCR tubes (Fisher Scientific, cat. no. 14–222-250) Axygen™ PCR plates, 96 well (VWR, cat. no. PCR-96M2-HS-C) 384-well optical plate (e.g., Roche, LightCycler 480 Multiwell plate 384, cat. no. 5102430001) Axygen 1.5 mL Boil-Proof Microcentrifuge Tubes, (VWR, cat. no. 10011–702) Falcon tubes, polypropylene, 15 mL (Corning cat. no. 352097) Falcon tubes, polypropylene, 50 mL (Corning, cat. no. 352070) Eppendorf Safe-lock microcentrifuge tubes, 1.5 mL (Eppendorf, cat. no. T9661–1000EA) Filtered sterile pipette tips (e.g., Rainin) Corning bottle-top vacuum filter system, 0.22 μM (Sigma-Aldrich, cat. no. CLS431098) Stericup filter unit, 0.45 μM (Millipore, cat. no. SCHVU02RE) Amicon Ultra-15 Centrifugal Filter Unit with Ultracel-50 membrane (Sigma-Aldrich, cat. no. UFC905024) Thermocycler with programmable temperature stepping functionality, 96 well (e.g., Applied Biosystems Veriti, cat. no. 4375786) Desktop microcentrifuges (e.g., Eppendorf, cat. no. 5424) Digital gel imaging system (GelDoc EZ, Bio-Rad, cat. no. 170–8270) and white sample tray (Bio-Rad, cat. no. 170–8272) ) UV spectrophotometer (e.g., NanoDrop 2000c, Thermo Scientific) Plate spectrophotometer (e.g., Synergy H4 Hybrid Multi-Mode Microplate Reader, BioTek) .. Reconstitute the LB Broth with agar at a concentration of 35 g L−1 in deionized water and swirl to mix.

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86
    Axygen boil proof microcentrifuge tube
    Boil Proof Microcentrifuge Tube, supplied by Axygen, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/boil proof microcentrifuge tube/product/Axygen
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    boil proof microcentrifuge tube - by Bioz Stars, 2021-06
    86/100 stars
      Buy from Supplier

    Image Search Results