bodo sp  (ATCC)


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    ATCC bodo sp
    Bodo Sp, supplied by ATCC, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bodo sp/product/ATCC
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bodo sp - by Bioz Stars, 2022-12
    88/100 stars

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    ATCC bodo sp 20130205t111427
    Epifluorescence micrographs of transformed marine protists. Representative images of transformants and wild-type cell lines of ten selected protist species. Colored boxes behind species names refer to phylogenetic supergroup assignments given in Fig. 1 . Representative data of at least two independent experiments are shown. The fluorescent images show the expression of individual fluorescent marker genes introduced via transformation for all organisms shown, except in the case of A. amoebiformis . For this, red depicts the natural autofluorescence of photosynthetic pigments in the cell, while the additional green spheres in the transformant fluorescence panel shows introduced GFP fluorescence (see Supplementary Fig. 15c for a trace of these different regions in the cell). Scale bars are as follows: 10 µm for A. amoebiformis , T. pseudonana , A. limacinum , B. <t>saltans</t> , N. gruberi , A. whisleri and S. rosetta ; 15 µm for P. marinus ; 20 µm for F. cylindrus and 100 µm for P. multiseries .
    Bodo Sp 20130205t111427, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bodo sp 20130205t111427/product/ATCC
    Average 92 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    bodo sp 20130205t111427 - by Bioz Stars, 2022-12
    92/100 stars
      Buy from Supplier

    88
    ATCC bodo sp
    Epifluorescence micrographs of transformed marine protists. Representative images of transformants and wild-type cell lines of ten selected protist species. Colored boxes behind species names refer to phylogenetic supergroup assignments given in Fig. 1 . Representative data of at least two independent experiments are shown. The fluorescent images show the expression of individual fluorescent marker genes introduced via transformation for all organisms shown, except in the case of A. amoebiformis . For this, red depicts the natural autofluorescence of photosynthetic pigments in the cell, while the additional green spheres in the transformant fluorescence panel shows introduced GFP fluorescence (see Supplementary Fig. 15c for a trace of these different regions in the cell). Scale bars are as follows: 10 µm for A. amoebiformis , T. pseudonana , A. limacinum , B. <t>saltans</t> , N. gruberi , A. whisleri and S. rosetta ; 15 µm for P. marinus ; 20 µm for F. cylindrus and 100 µm for P. multiseries .
    Bodo Sp, supplied by ATCC, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bodo sp/product/ATCC
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bodo sp - by Bioz Stars, 2022-12
    88/100 stars
      Buy from Supplier

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    Epifluorescence micrographs of transformed marine protists. Representative images of transformants and wild-type cell lines of ten selected protist species. Colored boxes behind species names refer to phylogenetic supergroup assignments given in Fig. 1 . Representative data of at least two independent experiments are shown. The fluorescent images show the expression of individual fluorescent marker genes introduced via transformation for all organisms shown, except in the case of A. amoebiformis . For this, red depicts the natural autofluorescence of photosynthetic pigments in the cell, while the additional green spheres in the transformant fluorescence panel shows introduced GFP fluorescence (see Supplementary Fig. 15c for a trace of these different regions in the cell). Scale bars are as follows: 10 µm for A. amoebiformis , T. pseudonana , A. limacinum , B. saltans , N. gruberi , A. whisleri and S. rosetta ; 15 µm for P. marinus ; 20 µm for F. cylindrus and 100 µm for P. multiseries .

    Journal: Nature Methods

    Article Title: Genetic tool development in marine protists: emerging model organisms for experimental cell biology

    doi: 10.1038/s41592-020-0796-x

    Figure Lengend Snippet: Epifluorescence micrographs of transformed marine protists. Representative images of transformants and wild-type cell lines of ten selected protist species. Colored boxes behind species names refer to phylogenetic supergroup assignments given in Fig. 1 . Representative data of at least two independent experiments are shown. The fluorescent images show the expression of individual fluorescent marker genes introduced via transformation for all organisms shown, except in the case of A. amoebiformis . For this, red depicts the natural autofluorescence of photosynthetic pigments in the cell, while the additional green spheres in the transformant fluorescence panel shows introduced GFP fluorescence (see Supplementary Fig. 15c for a trace of these different regions in the cell). Scale bars are as follows: 10 µm for A. amoebiformis , T. pseudonana , A. limacinum , B. saltans , N. gruberi , A. whisleri and S. rosetta ; 15 µm for P. marinus ; 20 µm for F. cylindrus and 100 µm for P. multiseries .

    Article Snippet: B. saltans (ATCC 30904) was transformed with a plasmid containing a cassette designed to fuse an endogenous EF1-α gene with eGFP for C-terminal tagging.

    Techniques: Transformation Assay, Expressing, Marker, Fluorescence

    Various methods were used to demonstrate successful transformation in different species: RT–PCR, western blot and sequencing. a – j , Western blot, RT–PCR or sequencing (in case of Cas9-induced excision by CRISPR) were used to verify expression of introduced constructs in one haptophyte: I. galbana ( a ), one rhizarian— A. amoebiformis ( b ), two stramenopiles— F. cylindrus ( c ) and P. tricornutum ( d ), three alveolates— K. veneficum ( e ), P. marinus ( f ) and A. carterae ( g ), two discobans— B. saltans ( h ) and D. papillatum ( i ) and one opisthokont— A. whisleri ( j ). Note that nptII/neo is used synonymously with amino 3′-glycosyl phosphotransferase gene ( aph (3′)) conferring resistance to kanamycin and neomycin. Representative data of at least two independent experiments are shown. For a detailed figure description see Supplementary Notes 2 . Source data

    Journal: Nature Methods

    Article Title: Genetic tool development in marine protists: emerging model organisms for experimental cell biology

    doi: 10.1038/s41592-020-0796-x

    Figure Lengend Snippet: Various methods were used to demonstrate successful transformation in different species: RT–PCR, western blot and sequencing. a – j , Western blot, RT–PCR or sequencing (in case of Cas9-induced excision by CRISPR) were used to verify expression of introduced constructs in one haptophyte: I. galbana ( a ), one rhizarian— A. amoebiformis ( b ), two stramenopiles— F. cylindrus ( c ) and P. tricornutum ( d ), three alveolates— K. veneficum ( e ), P. marinus ( f ) and A. carterae ( g ), two discobans— B. saltans ( h ) and D. papillatum ( i ) and one opisthokont— A. whisleri ( j ). Note that nptII/neo is used synonymously with amino 3′-glycosyl phosphotransferase gene ( aph (3′)) conferring resistance to kanamycin and neomycin. Representative data of at least two independent experiments are shown. For a detailed figure description see Supplementary Notes 2 . Source data

    Article Snippet: B. saltans (ATCC 30904) was transformed with a plasmid containing a cassette designed to fuse an endogenous EF1-α gene with eGFP for C-terminal tagging.

    Techniques: Transformation Assay, Reverse Transcription Polymerase Chain Reaction, Western Blot, Sequencing, CRISPR, Expressing, Construct