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Alomone Labs p2x7 n a unconjugated alomone labs blp pr004 wb
P2x7 N A Unconjugated Alomone Labs Blp Pr004 Wb, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/blp-pr004/pm40128095-81-83-86?v=Alomone+Labs
Average 93 stars, based on 3 article reviews

p2x7 n a unconjugated alomone labs blp pr004 wb - by Bioz Stars, 2026-07

93/100 stars

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Journal: Function

Article Title: Role of Pannexin 1, P2X7, and CFTR in ATP Release and Autocrine Signaling by Principal Cells of the Epididymis

doi: 10.1093/function/zqaf016

Figure Lengend Snippet: List of Antibodies

Article Snippet: P2X7 , N/A , Unconjugated , Alomone Labs , BLP-PR004 , WB; IF , 2 µg/mL; 30 µg/mL.

Techniques: Concentration Assay

Journal: Function

Article Title: Role of Pannexin 1, P2X7, and CFTR in ATP Release and Autocrine Signaling by Principal Cells of the Epididymis

doi: 10.1093/function/zqaf016

Figure Lengend Snippet: List of Antibodies

Article Snippet: P2X7 , N/A , Unconjugated , Alomone Labs , BLP-PR004 , WB; IF , 2 µg/mL; 30 µg/mL.

Techniques: Concentration Assay

Relative expression evaluated using real-time PCR and the 2 -ΔΔCT method for transcripts coding for P2Y2, P2Y4, P2Y6, P2Y11 and P2X7 receptors in SKOV-3 cells, normalized against HOSE6-3 levels. The housekeeping transcript B2M (beta-2-microglobulin) was used. The graph shows the mean ± S.E.M. of 4 independent experiments ***p<0.01 Student´s-t test.

Journal: PLOS ONE

Article Title: Extracellular ATP/P2X7 receptor, a regulatory axis of migration in ovarian carcinoma-derived cells

doi: 10.1371/journal.pone.0304062

Figure Lengend Snippet: Relative expression evaluated using real-time PCR and the 2 -ΔΔCT method for transcripts coding for P2Y2, P2Y4, P2Y6, P2Y11 and P2X7 receptors in SKOV-3 cells, normalized against HOSE6-3 levels. The housekeeping transcript B2M (beta-2-microglobulin) was used. The graph shows the mean ± S.E.M. of 4 independent experiments ***p<0.01 Student´s-t test.

Article Snippet: P2X7 receptor was detected by western blot with two antibodies, one directed against COOH-end (Alomone, Jerusalen, Israel, #APR-004) of and other against the extracellular loop of the receptor (Alomone, Jerusalen, Israel, #APR-008).

Techniques: Expressing, Real-time Polymerase Chain Reaction

A) Representative picture of P2X7 receptor expression in a serous carcinoma biopsy, from human protein atlas . The positive signal is in brown and the tissue stain is in blue. The tissues were analyzed with the validated antibody HPA044141. B-D) Relationship between the expression level of the P2RX7 transcript and the probability of survival of patients with ovarian carcinoma created with data from the Kaplan-Meier Plotter Database. Patients are classified as follows: B) Serous ovarian carcinoma patients (361 low expression and 743 high expression) C) Serous ovarian carcinoma patients stages 2+3 (294 low expression and 609 high expression) and C) Serous ovarian carcinoma patients stage 3+4 (333 low expression and 668 high expression).

Journal: PLOS ONE

Article Title: Extracellular ATP/P2X7 receptor, a regulatory axis of migration in ovarian carcinoma-derived cells

doi: 10.1371/journal.pone.0304062

Figure Lengend Snippet: A) Representative picture of P2X7 receptor expression in a serous carcinoma biopsy, from human protein atlas . The positive signal is in brown and the tissue stain is in blue. The tissues were analyzed with the validated antibody HPA044141. B-D) Relationship between the expression level of the P2RX7 transcript and the probability of survival of patients with ovarian carcinoma created with data from the Kaplan-Meier Plotter Database. Patients are classified as follows: B) Serous ovarian carcinoma patients (361 low expression and 743 high expression) C) Serous ovarian carcinoma patients stages 2+3 (294 low expression and 609 high expression) and C) Serous ovarian carcinoma patients stage 3+4 (333 low expression and 668 high expression).

Techniques: Expressing, Staining

A) The P2RX7 transcript was analyzed by reverse transcription followed by end-point PCR. Amplicons were analyzed in 1.5% agarose gels, purified and sequenced to confirm their identity. B) Western blot detection of P2X7 receptor in a preparation enriched in plasmatic membrane proteins from SKOV-3 cells, the protein was detected with an antibody directed against COOH-end (left) and other to the extracellular loop (right) C) Sequence of fluorescent images from SKOV-3 cells loaded with Fluo4-AM 2 μM showing changes in [Ca 2+ ] i before and after BzATP 50 μM addition (red arrow) in N-Ca 2+ and Z-Ca 2+ in the absence or presence of the P2X7 antagonist A438079 125 nM (aA43); pseudocolors from black to red represent low to high [Ca2+] i , respectively; time frames are indicated in minutes. D) Representative Ca 2+ fluorescence (Fluo-4) traces from SKOV-3 cells obtained in N-Ca 2+ , Z-Ca 2+ and Z-Ca 2+ plus A438079 125nM (aA43). Cells were stimulated with 50 μM BzATP in N-Ca 2+ or Z-Ca 2+ extracellular solutions in the presence or absence of the P2X7 receptor antagonist aA43; at the end of the protocol, ionomycin (10μM) and MnCl 2 (5mM) were sequentially applied to determine the maximum and minimum levels of intracellular Ca 2+ , respectively. E) Mean ± S.D. of the slope displayed by the sustained component of the Ca 2+ response induced by BzATP in N-Ca 2+ , Z-Ca2+ and Z-Ca 2+ plus aA43 observed in SKOV-3 cells. At least 100 cells were analyzed per experiment, n = 3.

Journal: PLOS ONE

Article Title: Extracellular ATP/P2X7 receptor, a regulatory axis of migration in ovarian carcinoma-derived cells

doi: 10.1371/journal.pone.0304062

Figure Lengend Snippet: A) The P2RX7 transcript was analyzed by reverse transcription followed by end-point PCR. Amplicons were analyzed in 1.5% agarose gels, purified and sequenced to confirm their identity. B) Western blot detection of P2X7 receptor in a preparation enriched in plasmatic membrane proteins from SKOV-3 cells, the protein was detected with an antibody directed against COOH-end (left) and other to the extracellular loop (right) C) Sequence of fluorescent images from SKOV-3 cells loaded with Fluo4-AM 2 μM showing changes in [Ca 2+ ] i before and after BzATP 50 μM addition (red arrow) in N-Ca 2+ and Z-Ca 2+ in the absence or presence of the P2X7 antagonist A438079 125 nM (aA43); pseudocolors from black to red represent low to high [Ca2+] i , respectively; time frames are indicated in minutes. D) Representative Ca 2+ fluorescence (Fluo-4) traces from SKOV-3 cells obtained in N-Ca 2+ , Z-Ca 2+ and Z-Ca 2+ plus A438079 125nM (aA43). Cells were stimulated with 50 μM BzATP in N-Ca 2+ or Z-Ca 2+ extracellular solutions in the presence or absence of the P2X7 receptor antagonist aA43; at the end of the protocol, ionomycin (10μM) and MnCl 2 (5mM) were sequentially applied to determine the maximum and minimum levels of intracellular Ca 2+ , respectively. E) Mean ± S.D. of the slope displayed by the sustained component of the Ca 2+ response induced by BzATP in N-Ca 2+ , Z-Ca2+ and Z-Ca 2+ plus aA43 observed in SKOV-3 cells. At least 100 cells were analyzed per experiment, n = 3.

Techniques: Reverse Transcription, Purification, Western Blot, Membrane, Sequencing, Fluorescence

A) P2RX7 and constitutive ( CYC ) transcripts were analyzed by RT-PCR in the SKOV-3 parental (Parental) and SKOV3-P2X7 KO (P2X7 KO ) cells, amplicons were analyzed in agarose 1.5% gels, primers were previously validated. B) Representative Ca 2+ fluorescence (Fluo-4) traces from either SKOV-Parental cell line and SKOV3-P2X7 KO cells. Cells were stimulated with 50μM BzATP in N-Ca 2+ extracellular solution; at the end of the protocol, ionomycin (10μM) and MnCl 2 (5mM) were sequentially applied to determine the maximum and minimum levels of intracellular Ca 2+ , respectively. C) SKOV-Parental line and SKOV3-P2X7 KO confluent cultures were stimulated with BzATP 50μM during 16h and cell migration was measured by the scratch assay. Representative pictures are shown at time 0h and 16h. In the graphs, bars represent the mean value ± S.E.M. of three different experiments. *p < 0.05.

Journal: PLOS ONE

Article Title: Extracellular ATP/P2X7 receptor, a regulatory axis of migration in ovarian carcinoma-derived cells

doi: 10.1371/journal.pone.0304062

Figure Lengend Snippet: A) P2RX7 and constitutive ( CYC ) transcripts were analyzed by RT-PCR in the SKOV-3 parental (Parental) and SKOV3-P2X7 KO (P2X7 KO ) cells, amplicons were analyzed in agarose 1.5% gels, primers were previously validated. B) Representative Ca 2+ fluorescence (Fluo-4) traces from either SKOV-Parental cell line and SKOV3-P2X7 KO cells. Cells were stimulated with 50μM BzATP in N-Ca 2+ extracellular solution; at the end of the protocol, ionomycin (10μM) and MnCl 2 (5mM) were sequentially applied to determine the maximum and minimum levels of intracellular Ca 2+ , respectively. C) SKOV-Parental line and SKOV3-P2X7 KO confluent cultures were stimulated with BzATP 50μM during 16h and cell migration was measured by the scratch assay. Representative pictures are shown at time 0h and 16h. In the graphs, bars represent the mean value ± S.E.M. of three different experiments. *p < 0.05.

Techniques: Reverse Transcription Polymerase Chain Reaction, Fluorescence, Migration, Wound Healing Assay

P2X 7 receptors were expressed by GFAP + ependymo-radial glial cells. Spinal cord cross sections are shown (dorsal is up; asterisk indicates central canal; scale bar: 50 μm). Representative images of GFAP and P2X 7 labelling in naïve fish (A-C′), at 7 dpi (D-F′), and at 14 dpi (G-I′). Colocalization (arrows) was seen in radial processes and cell bodies (C′,F′,I′). GFAP + cells that did not colocalize with P2X 7 were also detected (open arrowheads) (C′,F′,I′). n =3/group. Created with BioRender.com.

Journal: Biology Open

Article Title: P2X 7 regulates ependymo-radial glial cell proliferation in adult Danio rerio following spinal cord injury

doi: 10.1242/bio.060270

Figure Lengend Snippet: P2X 7 receptors were expressed by GFAP + ependymo-radial glial cells. Spinal cord cross sections are shown (dorsal is up; asterisk indicates central canal; scale bar: 50 μm). Representative images of GFAP and P2X 7 labelling in naïve fish (A-C′), at 7 dpi (D-F′), and at 14 dpi (G-I′). Colocalization (arrows) was seen in radial processes and cell bodies (C′,F′,I′). GFAP + cells that did not colocalize with P2X 7 were also detected (open arrowheads) (C′,F′,I′). n =3/group. Created with BioRender.com.

Article Snippet: This included a P2X 7 control antigen (Alomone Labs, BLP-PR004, Q64663).

Techniques:

P2X 7 receptors were expressed by PCNA + proliferating cells. Spinal cord cross sections are shown (dorsal is up; asterisk indicates central canal; scale bar: 50 μm). Representative images of PCNA + and P2X 7 labelling in naïve fish (A-C′), at 7 dpi rostral (D-F′), and at 7 dpi caudal (G-I′). Colocalization (arrows) was seen in some cells (C′,F′,I′). PCNA + cells that did not colocalize with P2X 7 were also detected (open arrowheads) (C′,F′,I′). n =3/group. Created with BioRender.com.

Journal: Biology Open

Article Title: P2X 7 regulates ependymo-radial glial cell proliferation in adult Danio rerio following spinal cord injury

doi: 10.1242/bio.060270

Figure Lengend Snippet: P2X 7 receptors were expressed by PCNA + proliferating cells. Spinal cord cross sections are shown (dorsal is up; asterisk indicates central canal; scale bar: 50 μm). Representative images of PCNA + and P2X 7 labelling in naïve fish (A-C′), at 7 dpi rostral (D-F′), and at 7 dpi caudal (G-I′). Colocalization (arrows) was seen in some cells (C′,F′,I′). PCNA + cells that did not colocalize with P2X 7 were also detected (open arrowheads) (C′,F′,I′). n =3/group. Created with BioRender.com.

Article Snippet: This included a P2X 7 control antigen (Alomone Labs, BLP-PR004, Q64663).

Techniques:

P2X 7 receptors were expressed by HuC/D + neurons. Spinal cord cross sections are shown (dorsal is up; asterisk indicates central canal; scale bar: 50 μm). Representative images of HuC/D + and P2X 7 labelling in naïve fish (A-C′), at 14 dpi rostral (D-F′), and at 14 dpi caudal (G-I′). Colocalization (arrows) was seen in larger mature neurons and smaller immature neurons (C′,F′,I′). n =3/group. Created with BioRender.com.

Journal: Biology Open

Article Title: P2X 7 regulates ependymo-radial glial cell proliferation in adult Danio rerio following spinal cord injury

doi: 10.1242/bio.060270

Figure Lengend Snippet: P2X 7 receptors were expressed by HuC/D + neurons. Spinal cord cross sections are shown (dorsal is up; asterisk indicates central canal; scale bar: 50 μm). Representative images of HuC/D + and P2X 7 labelling in naïve fish (A-C′), at 14 dpi rostral (D-F′), and at 14 dpi caudal (G-I′). Colocalization (arrows) was seen in larger mature neurons and smaller immature neurons (C′,F′,I′). n =3/group. Created with BioRender.com.

Article Snippet: This included a P2X 7 control antigen (Alomone Labs, BLP-PR004, Q64663).

Techniques:

Temporal changes in P2X 7 protein expression following SCI in adult zebrafish. Schematic representations of naïve and injured spinal cord areas of analysis (A-B), as well as tissue collection timeline (C). Representative western blot and corresponding total protein in naïve zebrafish (Zf) spinal cord tissue, mouse (Ms) hippocampal tissue as a positive control (+), and pre-absorption of naïve zebrafish spinal cord tissue with a blocking peptide as a negative control (-) (D). Representative western blot and corresponding total protein at 1 dpi (E), 7 dpi (G), and 14 dpi (I). Quantitative analysis of the 50 kDa isoform showed significant downregulation within the injury (In, n =10, one-way ANOVA with Dunnett's post hoc test, P =0.0079) and caudal to the lesion (Ca, n =9, P =0.0046) at 7 dpi when normalized to naïve tissue ( n =9) (H). Protein expression of the 50 kDa isoform showed basal levels of expression at 1 dpi (F) and 14 dpi (J). Data presented as means±s.d. **, significant differences, P <0.01. Created with BioRender.com.

Journal: Biology Open

Article Title: P2X 7 regulates ependymo-radial glial cell proliferation in adult Danio rerio following spinal cord injury

doi: 10.1242/bio.060270

Figure Lengend Snippet: Temporal changes in P2X 7 protein expression following SCI in adult zebrafish. Schematic representations of naïve and injured spinal cord areas of analysis (A-B), as well as tissue collection timeline (C). Representative western blot and corresponding total protein in naïve zebrafish (Zf) spinal cord tissue, mouse (Ms) hippocampal tissue as a positive control (+), and pre-absorption of naïve zebrafish spinal cord tissue with a blocking peptide as a negative control (-) (D). Representative western blot and corresponding total protein at 1 dpi (E), 7 dpi (G), and 14 dpi (I). Quantitative analysis of the 50 kDa isoform showed significant downregulation within the injury (In, n =10, one-way ANOVA with Dunnett's post hoc test, P =0.0079) and caudal to the lesion (Ca, n =9, P =0.0046) at 7 dpi when normalized to naïve tissue ( n =9) (H). Protein expression of the 50 kDa isoform showed basal levels of expression at 1 dpi (F) and 14 dpi (J). Data presented as means±s.d. **, significant differences, P <0.01. Created with BioRender.com.

Article Snippet: This included a P2X 7 control antigen (Alomone Labs, BLP-PR004, Q64663).

Techniques: Expressing, Western Blot, Positive Control, Blocking Assay, Negative Control

Surgery increased P2X7 receptor and caspase 1 expression. Hippocampus was harvested 6 h after the surgery for immunohistochemical staining or 24 h after the surgery for Western blotting. A: representative images of immunostaining. Scale bar = 100 µm. B: representative images of Western blotting. C: quantitative data of Western blotting. Results are mean ± S.E.M. (n = 5 – 6). * P < 0.05 compared with control group.

Journal: Brain, behavior, and immunity

Article Title: Critical role of P2X7 receptors in the neuroinflammation and cognitive dysfunction after surgery

doi: 10.1016/j.bbi.2017.01.005

Figure Lengend Snippet: Surgery increased P2X7 receptor and caspase 1 expression. Hippocampus was harvested 6 h after the surgery for immunohistochemical staining or 24 h after the surgery for Western blotting. A: representative images of immunostaining. Scale bar = 100 µm. B: representative images of Western blotting. C: quantitative data of Western blotting. Results are mean ± S.E.M. (n = 5 – 6). * P < 0.05 compared with control group.

Article Snippet: To stain P2X7 receptors, the rabbit anti-P2X7 antibody (1:200; Alomone labs) and the donkey anti-rabbit IgG antibody conjugated with NL557 (1:200; R&D Systems) were applied.

Techniques: Expressing, Immunohistochemical staining, Staining, Western Blot, Immunostaining

Inhibition of P2X7 receptors attenuated surgery-induced learning and memory impairment. Mice were started to be tested by Barnes maze and fear conditioning 2 weeks after the surgery under isoflurane anesthesia. A: training sessions of Barnes maze. Results are mean ± S.E.M. (n = 12). * P < 0.05 compared with the corresponding data on day 1. B: memory phase of Barnes maze. Results are in box plot format (n = 12). •: lowest or highest score (the score will not show up if it falls in the 95th percentile); between lines: 95th percentile of the data; inside boxes: 25th to 75th percentile including the median of the data. ^ P < 0.05 compared with control group. # P < 0.05 compared with surgery plus isoflurane anesthesia group. C: Fear conditioning. Results are mean ± S.E.M. (n = 11 – 12). ^ P < 0.05 compared with control group; # P < 0.05 compared with surgery plus isoflurane anesthesia.

Journal: Brain, behavior, and immunity

Article Title: Critical role of P2X7 receptors in the neuroinflammation and cognitive dysfunction after surgery

doi: 10.1016/j.bbi.2017.01.005

Figure Lengend Snippet: Inhibition of P2X7 receptors attenuated surgery-induced learning and memory impairment. Mice were started to be tested by Barnes maze and fear conditioning 2 weeks after the surgery under isoflurane anesthesia. A: training sessions of Barnes maze. Results are mean ± S.E.M. (n = 12). * P < 0.05 compared with the corresponding data on day 1. B: memory phase of Barnes maze. Results are in box plot format (n = 12). •: lowest or highest score (the score will not show up if it falls in the 95th percentile); between lines: 95th percentile of the data; inside boxes: 25th to 75th percentile including the median of the data. ^ P < 0.05 compared with control group. # P < 0.05 compared with surgery plus isoflurane anesthesia group. C: Fear conditioning. Results are mean ± S.E.M. (n = 11 – 12). ^ P < 0.05 compared with control group; # P < 0.05 compared with surgery plus isoflurane anesthesia.

Article Snippet: To stain P2X7 receptors, the rabbit anti-P2X7 antibody (1:200; Alomone labs) and the donkey anti-rabbit IgG antibody conjugated with NL557 (1:200; R&D Systems) were applied.

Techniques: Inhibition

Inhibition of P2X7 receptors attenuated surgery-induced neuroinflammation. Hippocampus was harvested 6 h or 7 days after the surgery. A: representative images of Iba-1 staining of hippocampus harvested 6 h after the surgery. Scale bar = 100 µm. B: quantitative data of Iba-1 of hippocampus harvested 6 h after the surgery. C: representative images of Iba-1 staining of hippocampus harvested 7 days after the surgery. D: quantitative data of Iba-1 of hippocampus harvested 7 days after the surgery. Results are mean ± S.E.M. (n = 5 for panels B and D). * P < 0.05 compared with control group; ^ P < 0.05 compared with surgery plus isoflurane anesthesia group.

Journal: Brain, behavior, and immunity

Article Title: Critical role of P2X7 receptors in the neuroinflammation and cognitive dysfunction after surgery

doi: 10.1016/j.bbi.2017.01.005

Figure Lengend Snippet: Inhibition of P2X7 receptors attenuated surgery-induced neuroinflammation. Hippocampus was harvested 6 h or 7 days after the surgery. A: representative images of Iba-1 staining of hippocampus harvested 6 h after the surgery. Scale bar = 100 µm. B: quantitative data of Iba-1 of hippocampus harvested 6 h after the surgery. C: representative images of Iba-1 staining of hippocampus harvested 7 days after the surgery. D: quantitative data of Iba-1 of hippocampus harvested 7 days after the surgery. Results are mean ± S.E.M. (n = 5 for panels B and D). * P < 0.05 compared with control group; ^ P < 0.05 compared with surgery plus isoflurane anesthesia group.

Article Snippet: To stain P2X7 receptors, the rabbit anti-P2X7 antibody (1:200; Alomone labs) and the donkey anti-rabbit IgG antibody conjugated with NL557 (1:200; R&D Systems) were applied.

Techniques: Inhibition, Staining

Inhibition of P2X7 receptors attenuated surgery-induced increase of P2X7 receptor and caspase 1 expression. Hippocampus was harvested 6 h or 7 days after the surgery under isoflurane anesthesia. A and B: representative images of Western blotting. C: quantitative data of P2X7 receptors. D: quantitative data of P20. E: quantitative data of precursor caspase 1. Results are mean ± S.E.M. (n = 6). * P < 0.05 compared with control group; ^ P < 0.05 compared with the corresponding surgery plus isoflurane anesthesia group; # P < 0.05 compared with the level of P20 at 6 h after surgery under isoflurane anesthesia.

Journal: Brain, behavior, and immunity

Article Title: Critical role of P2X7 receptors in the neuroinflammation and cognitive dysfunction after surgery

doi: 10.1016/j.bbi.2017.01.005

Figure Lengend Snippet: Inhibition of P2X7 receptors attenuated surgery-induced increase of P2X7 receptor and caspase 1 expression. Hippocampus was harvested 6 h or 7 days after the surgery under isoflurane anesthesia. A and B: representative images of Western blotting. C: quantitative data of P2X7 receptors. D: quantitative data of P20. E: quantitative data of precursor caspase 1. Results are mean ± S.E.M. (n = 6). * P < 0.05 compared with control group; ^ P < 0.05 compared with the corresponding surgery plus isoflurane anesthesia group; # P < 0.05 compared with the level of P20 at 6 h after surgery under isoflurane anesthesia.

Article Snippet: To stain P2X7 receptors, the rabbit anti-P2X7 antibody (1:200; Alomone labs) and the donkey anti-rabbit IgG antibody conjugated with NL557 (1:200; R&D Systems) were applied.

Techniques: Inhibition, Expressing, Western Blot

P2X7 receptor knockout blocked surgery-induced neuroinflammation and cognitive impairment. Cerebral cortex and hippocampus of C57BL/6J mice or P2X7 receptor knockout mice were harvested for Western blotting of P2X7 receptors. Also, P2X7 receptor knockout mice were started to be tested by Barnes maze and fear conditioning 2 weeks after the surgery under isoflurane anesthesia. Mouse hippocampus was harvested 6 h after the surgery for measuring IL-1β. A: Western blot images of P2X7 receptor. B: training sessions of Barnes maze. Results are mean ± S.E.M. (n = 8). * P < 0.05 compared with the corresponding data on day 1. C: memory phase of Barnes maze. Results are in box plot format (n = 8). •: lowest or highest score (the score will not show up if it falls in the 95th percentile); between lines: 95th percentile of the data; inside boxes: 25th to 75th percentile including the median of the data. D: Fear conditioning. Results are mean ± S.E.M. (n = 8). E: IL-1β concentrations in the hippocampus. Results are mean ± S.E.M. (n = 5).

Journal: Brain, behavior, and immunity

Article Title: Critical role of P2X7 receptors in the neuroinflammation and cognitive dysfunction after surgery

doi: 10.1016/j.bbi.2017.01.005

Figure Lengend Snippet: P2X7 receptor knockout blocked surgery-induced neuroinflammation and cognitive impairment. Cerebral cortex and hippocampus of C57BL/6J mice or P2X7 receptor knockout mice were harvested for Western blotting of P2X7 receptors. Also, P2X7 receptor knockout mice were started to be tested by Barnes maze and fear conditioning 2 weeks after the surgery under isoflurane anesthesia. Mouse hippocampus was harvested 6 h after the surgery for measuring IL-1β. A: Western blot images of P2X7 receptor. B: training sessions of Barnes maze. Results are mean ± S.E.M. (n = 8). * P < 0.05 compared with the corresponding data on day 1. C: memory phase of Barnes maze. Results are in box plot format (n = 8). •: lowest or highest score (the score will not show up if it falls in the 95th percentile); between lines: 95th percentile of the data; inside boxes: 25th to 75th percentile including the median of the data. D: Fear conditioning. Results are mean ± S.E.M. (n = 8). E: IL-1β concentrations in the hippocampus. Results are mean ± S.E.M. (n = 5).

Article Snippet: To stain P2X7 receptors, the rabbit anti-P2X7 antibody (1:200; Alomone labs) and the donkey anti-rabbit IgG antibody conjugated with NL557 (1:200; R&D Systems) were applied.

Techniques: Knock-Out, Western Blot

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