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trpm7 blocking peptide  (Alomone Labs)


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    Alomone Labs trpm7 blocking peptide
    Trpm7 Blocking Peptide, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/blp-cc047/custom%40blp-cc047%4036499188?v=Alomone+Labs
    Average 90 stars, based on 1 article reviews
    trpm7 blocking peptide - by Bioz Stars, 2026-07
    90/100 stars

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    trpm7 blocking peptide  (Alomone Labs)
    90
    Alomone Labs trpm7 blocking peptide
    Trpm7 Blocking Peptide, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/blp-cc047/custom%40blp-cc047%4036499188?v=Alomone+Labs
    Average 90 stars, based on 1 article reviews
    trpm7 blocking peptide - by Bioz Stars, 2026-07
    90/100 stars
    		  Buy from Supplier
    anti trpm7 antibody  (Alomone Labs)
    90
    Alomone Labs anti trpm7 antibody
    Specificity of antibody-based detection of TRPM6 and TPRM7 in pig right atrial (RA) cardiomyocytes: ( A , B ) Immunofluorescence signals in cells incubated with anti-TRPM6 (red, when using Alexa Fluor 546) or <t>anti-TRPM7</t> (green, when using Alexa Fluor 488) antibodies used alone (upper panels). The immunofluorescence of TRPM6 was increased post-acquisition by a factor of 2 for better visualization of the red staining. Bottom panels show merged images in the same cells stained with either anti-TRPM6 or anti-TRPM7 as well as Phalloidin-CF 405 for filamentous actin (F-actin) cytoskeleton (in surrogate grey) and Hoechst 33342 for nuclei (in surrogate white); ( C , D ) immunofluorescence signals in cells incubated with anti-TRPM6 or anti-TRPM7 antibodies in the presence of antibody-blocking peptides (upper panels). Bottom panels show merged images in the same cells stained with either anti-TRPM6 or anti-TRPM7, together with the blocking peptides, as well as Phalloidin-CF 405 for F-actin cytoskeleton (in surrogate grey) and Hoechst 33342 for nuclei (in surrogate white). There was no staining with anti-TRPM6 (red) or anti-TRPM7 (green) in the presence of antibody-blocking peptides; ( E , F ) immunofluorescence signals in cells incubated with anti-TRPM6 antibodies in the presence of the TRPM7-blocking peptide or with anti-TRPM7 antibodies in the presence of the TRPM6-blocking peptide (upper panels). Bottom panels show merged images in the same cells stained with the anti-TRPM antibodies together with the off-target blocking peptides, as well as Phalloidin-CF 405 for F-actin cytoskeleton (in surrogate grey) and Hoechst 33342 for nuclei (in surrogate white). Staining was similar to that in conditions where there is no blocking peptide. Scale bars indicate 20 µm.
    Anti Trpm7 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/blp-cc047/pmc09736228-142-4-17?v=Alomone+Labs
    Average 90 stars, based on 1 article reviews
    anti trpm7 antibody - by Bioz Stars, 2026-07
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Specificity of antibody-based detection of TRPM6 and TPRM7 in pig right atrial (RA) cardiomyocytes: ( A , B ) Immunofluorescence signals in cells incubated with anti-TRPM6 (red, when using Alexa Fluor 546) or anti-TRPM7 (green, when using Alexa Fluor 488) antibodies used alone (upper panels). The immunofluorescence of TRPM6 was increased post-acquisition by a factor of 2 for better visualization of the red staining. Bottom panels show merged images in the same cells stained with either anti-TRPM6 or anti-TRPM7 as well as Phalloidin-CF 405 for filamentous actin (F-actin) cytoskeleton (in surrogate grey) and Hoechst 33342 for nuclei (in surrogate white); ( C , D ) immunofluorescence signals in cells incubated with anti-TRPM6 or anti-TRPM7 antibodies in the presence of antibody-blocking peptides (upper panels). Bottom panels show merged images in the same cells stained with either anti-TRPM6 or anti-TRPM7, together with the blocking peptides, as well as Phalloidin-CF 405 for F-actin cytoskeleton (in surrogate grey) and Hoechst 33342 for nuclei (in surrogate white). There was no staining with anti-TRPM6 (red) or anti-TRPM7 (green) in the presence of antibody-blocking peptides; ( E , F ) immunofluorescence signals in cells incubated with anti-TRPM6 antibodies in the presence of the TRPM7-blocking peptide or with anti-TRPM7 antibodies in the presence of the TRPM6-blocking peptide (upper panels). Bottom panels show merged images in the same cells stained with the anti-TRPM antibodies together with the off-target blocking peptides, as well as Phalloidin-CF 405 for F-actin cytoskeleton (in surrogate grey) and Hoechst 33342 for nuclei (in surrogate white). Staining was similar to that in conditions where there is no blocking peptide. Scale bars indicate 20 µm.

    Journal: International Journal of Molecular Sciences

    Article Title: Detection of TRPM6 and TRPM7 Proteins in Normal and Diseased Cardiac Atrial Tissue and Isolated Cardiomyocytes

    doi: 10.3390/ijms232314860

    Figure Lengend Snippet: Specificity of antibody-based detection of TRPM6 and TPRM7 in pig right atrial (RA) cardiomyocytes: ( A , B ) Immunofluorescence signals in cells incubated with anti-TRPM6 (red, when using Alexa Fluor 546) or anti-TRPM7 (green, when using Alexa Fluor 488) antibodies used alone (upper panels). The immunofluorescence of TRPM6 was increased post-acquisition by a factor of 2 for better visualization of the red staining. Bottom panels show merged images in the same cells stained with either anti-TRPM6 or anti-TRPM7 as well as Phalloidin-CF 405 for filamentous actin (F-actin) cytoskeleton (in surrogate grey) and Hoechst 33342 for nuclei (in surrogate white); ( C , D ) immunofluorescence signals in cells incubated with anti-TRPM6 or anti-TRPM7 antibodies in the presence of antibody-blocking peptides (upper panels). Bottom panels show merged images in the same cells stained with either anti-TRPM6 or anti-TRPM7, together with the blocking peptides, as well as Phalloidin-CF 405 for F-actin cytoskeleton (in surrogate grey) and Hoechst 33342 for nuclei (in surrogate white). There was no staining with anti-TRPM6 (red) or anti-TRPM7 (green) in the presence of antibody-blocking peptides; ( E , F ) immunofluorescence signals in cells incubated with anti-TRPM6 antibodies in the presence of the TRPM7-blocking peptide or with anti-TRPM7 antibodies in the presence of the TRPM6-blocking peptide (upper panels). Bottom panels show merged images in the same cells stained with the anti-TRPM antibodies together with the off-target blocking peptides, as well as Phalloidin-CF 405 for F-actin cytoskeleton (in surrogate grey) and Hoechst 33342 for nuclei (in surrogate white). Staining was similar to that in conditions where there is no blocking peptide. Scale bars indicate 20 µm.

    Article Snippet: The blocking peptide for anti-TRPM7 antibody (#BLP-CC047) and the blocking peptide for anti-TRPM6 (#BPL-CC046) was created by Alomone Labs for the antibody TRPM6 or TRPM7.

    Techniques: Immunofluorescence, Incubation, Staining, Blocking Assay

    Detection of TRPM6 and TRPM7 in the same cell and cellular localization of TRPM7: ( A , B ) Images of human RA cell illustrating the localization of TRPM6 (red) and TRPM7 (green) proteins in the same cardiomyocyte obtained using primary antibodies conjugated with Alexa Fluor 546 and Alexa Fluor 488, respectively, at different scanning layers [( A ), near the cell surface and ( B ), deeper in the cell towards the middle of its thickness]. Phalloidin-CF 405 for F-actin cytoskeleton and Hoechst 33342 for nuclei appear in surrogate grey; ( C , D ) immunofluorescence images of TRPM7 (stained in green when using Alexa Fluor 488) and F-actin cytoskeleton (stained in red when using Phalloidin-Alexa Fluor 546) were used for calculating fluorescence intensity in human ( C ) and pig ( D ) RA cardiomyocytes presented in ( E , F ). Hoechst 33342 for nuclei appears in blue. Scale bars indicate 20 µm; ( E , F ) constructed graphs of the fluorescence intensity using stacks of 12 slices for the localization of TRPM7 (green) and F-actin (red) along lines of interest on the cardiomyocytes merged images. The sampling distance in depth between scanned slices was 1 µm. The calculated correlation coefficient between F-actin and TRPM7 peaks (at a place as indicated) was 0.32 for human ( E ) and 0.11 for pig ( F ) RA cardiomyocytes.

    Journal: International Journal of Molecular Sciences

    Article Title: Detection of TRPM6 and TRPM7 Proteins in Normal and Diseased Cardiac Atrial Tissue and Isolated Cardiomyocytes

    doi: 10.3390/ijms232314860

    Figure Lengend Snippet: Detection of TRPM6 and TRPM7 in the same cell and cellular localization of TRPM7: ( A , B ) Images of human RA cell illustrating the localization of TRPM6 (red) and TRPM7 (green) proteins in the same cardiomyocyte obtained using primary antibodies conjugated with Alexa Fluor 546 and Alexa Fluor 488, respectively, at different scanning layers [( A ), near the cell surface and ( B ), deeper in the cell towards the middle of its thickness]. Phalloidin-CF 405 for F-actin cytoskeleton and Hoechst 33342 for nuclei appear in surrogate grey; ( C , D ) immunofluorescence images of TRPM7 (stained in green when using Alexa Fluor 488) and F-actin cytoskeleton (stained in red when using Phalloidin-Alexa Fluor 546) were used for calculating fluorescence intensity in human ( C ) and pig ( D ) RA cardiomyocytes presented in ( E , F ). Hoechst 33342 for nuclei appears in blue. Scale bars indicate 20 µm; ( E , F ) constructed graphs of the fluorescence intensity using stacks of 12 slices for the localization of TRPM7 (green) and F-actin (red) along lines of interest on the cardiomyocytes merged images. The sampling distance in depth between scanned slices was 1 µm. The calculated correlation coefficient between F-actin and TRPM7 peaks (at a place as indicated) was 0.32 for human ( E ) and 0.11 for pig ( F ) RA cardiomyocytes.

    Article Snippet: The blocking peptide for anti-TRPM7 antibody (#BLP-CC047) and the blocking peptide for anti-TRPM6 (#BPL-CC046) was created by Alomone Labs for the antibody TRPM6 or TRPM7.

    Techniques: Immunofluorescence, Staining, Fluorescence, Construct, Sampling

    Effect of TRP channel modulating drugs carvacrol (CAR) or 2-aminoethoxydiphenyl borate (2-APB) on detected TRPM6 and TRPM7 in pig and human RA cardiomyocytes: ( A – F ) Immunofluorescence of TRPM6 and TPRM7 in pig cardiomyocytes under control conditions and in the presence of CAR or 2-APB. Scale bars indicate 20 µm; ( G , H ) quantification of the immunodetected TRPM7 and TRPM6 proteins in pig RA cardiomyocytes in the absence (unfilled symbols) or in the presence (filled symbols) of either CAR ( G ) or 2-APB ( H ); ( I , J ) quantification of the immunodetected TRPM7 ( I ) and TRPM6 ( J ) proteins in human RA cardiomyocytes in the absence (unfilled symbols) or presence (filled symbols) of 2-APB at either 500 µM or 2 mM. Fluorescence intensity expressed in arbitrary units (a.u.). * p < 0.001 for drug vs. no drug. Notice opposite changes on TRPM7 with 2-APB at 500 µM vs. at 2 mM.

    Journal: International Journal of Molecular Sciences

    Article Title: Detection of TRPM6 and TRPM7 Proteins in Normal and Diseased Cardiac Atrial Tissue and Isolated Cardiomyocytes

    doi: 10.3390/ijms232314860

    Figure Lengend Snippet: Effect of TRP channel modulating drugs carvacrol (CAR) or 2-aminoethoxydiphenyl borate (2-APB) on detected TRPM6 and TRPM7 in pig and human RA cardiomyocytes: ( A – F ) Immunofluorescence of TRPM6 and TPRM7 in pig cardiomyocytes under control conditions and in the presence of CAR or 2-APB. Scale bars indicate 20 µm; ( G , H ) quantification of the immunodetected TRPM7 and TRPM6 proteins in pig RA cardiomyocytes in the absence (unfilled symbols) or in the presence (filled symbols) of either CAR ( G ) or 2-APB ( H ); ( I , J ) quantification of the immunodetected TRPM7 ( I ) and TRPM6 ( J ) proteins in human RA cardiomyocytes in the absence (unfilled symbols) or presence (filled symbols) of 2-APB at either 500 µM or 2 mM. Fluorescence intensity expressed in arbitrary units (a.u.). * p < 0.001 for drug vs. no drug. Notice opposite changes on TRPM7 with 2-APB at 500 µM vs. at 2 mM.

    Article Snippet: The blocking peptide for anti-TRPM7 antibody (#BLP-CC047) and the blocking peptide for anti-TRPM6 (#BPL-CC046) was created by Alomone Labs for the antibody TRPM6 or TRPM7.

    Techniques: Immunofluorescence, Fluorescence

    Tissue-level and subcellular localization of TRPM6 and TRPM7 by immunohistochemistry in the pig RA: ( A – D ) Confocal microscopy images obtained from perpendicular ( A , B ) and longitudinal ( C , D ) tissue slices. Images shown at higher magnification in the lower panels correspond to zoomed-in areas delimited by squares at a lower scale in the upper panels. TRPM6 and TRPM7 proteins were detected with AF-488-conjugated antibodies (green), the cell surface was labeled with AF-555 for wheat germ agglutinin (WGA, in red), whereas the nucleus staining (with Hoechst 33342) appears in blue. Notice that TRPM6 and TRPM7 are detected both at the cell membrane (filled arrows) and intracellularly (unfilled arrows). Scale bars indicate 20 µm.

    Journal: International Journal of Molecular Sciences

    Article Title: Detection of TRPM6 and TRPM7 Proteins in Normal and Diseased Cardiac Atrial Tissue and Isolated Cardiomyocytes

    doi: 10.3390/ijms232314860

    Figure Lengend Snippet: Tissue-level and subcellular localization of TRPM6 and TRPM7 by immunohistochemistry in the pig RA: ( A – D ) Confocal microscopy images obtained from perpendicular ( A , B ) and longitudinal ( C , D ) tissue slices. Images shown at higher magnification in the lower panels correspond to zoomed-in areas delimited by squares at a lower scale in the upper panels. TRPM6 and TRPM7 proteins were detected with AF-488-conjugated antibodies (green), the cell surface was labeled with AF-555 for wheat germ agglutinin (WGA, in red), whereas the nucleus staining (with Hoechst 33342) appears in blue. Notice that TRPM6 and TRPM7 are detected both at the cell membrane (filled arrows) and intracellularly (unfilled arrows). Scale bars indicate 20 µm.

    Article Snippet: The blocking peptide for anti-TRPM7 antibody (#BLP-CC047) and the blocking peptide for anti-TRPM6 (#BPL-CC046) was created by Alomone Labs for the antibody TRPM6 or TRPM7.

    Techniques: Immunohistochemistry, Confocal Microscopy, Labeling, Staining

    Tissue-level and subcellular localization of TRPM6 and TRPM7 by immunohistochemistry in the RA of an undiseased human heart from car victim. ( A – D ) Confocal microscopy images obtained from perpendicular ( A , B ) and longitudinal ( C , D ) tissue slices. Images shown at higher magnification in the lower panels correspond to zoomed-in areas delimited by squares at a lower scale in the upper panels. TRPM6 and TRPM7 proteins were detected with AF-488-conjugated antibodies (green), the cell surface was labeled with AF-555 for wheat germ agglutinin (WGA, in red), whereas the nucleus staining (with Hoechst 33342) appears in blue. Notice that TRPM6 and TRPM7 are detected both at the cell membrane (filled arrows) and intracellularly (unfilled arrows). Scale bars indicate 20 µm.

    Journal: International Journal of Molecular Sciences

    Article Title: Detection of TRPM6 and TRPM7 Proteins in Normal and Diseased Cardiac Atrial Tissue and Isolated Cardiomyocytes

    doi: 10.3390/ijms232314860

    Figure Lengend Snippet: Tissue-level and subcellular localization of TRPM6 and TRPM7 by immunohistochemistry in the RA of an undiseased human heart from car victim. ( A – D ) Confocal microscopy images obtained from perpendicular ( A , B ) and longitudinal ( C , D ) tissue slices. Images shown at higher magnification in the lower panels correspond to zoomed-in areas delimited by squares at a lower scale in the upper panels. TRPM6 and TRPM7 proteins were detected with AF-488-conjugated antibodies (green), the cell surface was labeled with AF-555 for wheat germ agglutinin (WGA, in red), whereas the nucleus staining (with Hoechst 33342) appears in blue. Notice that TRPM6 and TRPM7 are detected both at the cell membrane (filled arrows) and intracellularly (unfilled arrows). Scale bars indicate 20 µm.

    Article Snippet: The blocking peptide for anti-TRPM7 antibody (#BLP-CC047) and the blocking peptide for anti-TRPM6 (#BPL-CC046) was created by Alomone Labs for the antibody TRPM6 or TRPM7.

    Techniques: Immunohistochemistry, Confocal Microscopy, Labeling, Staining

    TRPM6 and TRPM7 detection by immunohistochemistry in diseased human RA tissue: ( A – D ) Representative images obtained from longitudinal slices of RA tissues from two different patients: an ischemic heart disease (IHD) patient ( A , B ) and an AF patient ( C , D ). The area labeled with the square bars corresponds to the images presented on a larger scale in the panels below them. AF-488 for the TRPM6 and TRPM7 protein appears in green. AF-555 for the cell membrane surface labeling with WGA appear in red, and Hoechst 33342 for the nucleus staining appear in blue. Arrows highlight the presence of TRP proteins. Notice, qualitatively, the amorphous distribution of TRPs and less colocalization with the cell membrane in both the IHD ( A , B ) and AF ( C , D ) hearts as compared with the control (car victim’s heart in ).

    Journal: International Journal of Molecular Sciences

    Article Title: Detection of TRPM6 and TRPM7 Proteins in Normal and Diseased Cardiac Atrial Tissue and Isolated Cardiomyocytes

    doi: 10.3390/ijms232314860

    Figure Lengend Snippet: TRPM6 and TRPM7 detection by immunohistochemistry in diseased human RA tissue: ( A – D ) Representative images obtained from longitudinal slices of RA tissues from two different patients: an ischemic heart disease (IHD) patient ( A , B ) and an AF patient ( C , D ). The area labeled with the square bars corresponds to the images presented on a larger scale in the panels below them. AF-488 for the TRPM6 and TRPM7 protein appears in green. AF-555 for the cell membrane surface labeling with WGA appear in red, and Hoechst 33342 for the nucleus staining appear in blue. Arrows highlight the presence of TRP proteins. Notice, qualitatively, the amorphous distribution of TRPs and less colocalization with the cell membrane in both the IHD ( A , B ) and AF ( C , D ) hearts as compared with the control (car victim’s heart in ).

    Article Snippet: The blocking peptide for anti-TRPM7 antibody (#BLP-CC047) and the blocking peptide for anti-TRPM6 (#BPL-CC046) was created by Alomone Labs for the antibody TRPM6 or TRPM7.

    Techniques: Immunohistochemistry, Labeling, Staining

    Effect of cardiac disease conditions on TRPM6 and TRPM7: ( A – C ) TRPM fluorescence intensity levels in RA cells ( A ) or tissues ( B , C ). Fluorescence intensity levels of TRPM6 (unfilled) and TRPM7 (filled) channels in isolated cardiomyocytes of the human heart, grouped according to the heart rhythm (SR vs. AF, ( A )) or according to the absence or presence of ischemic heart disease (non-IHD vs. IHD, ( B )). Fluorescence intensity levels of TRPM6 (unfilled) and TRPM7 (filled) channels in histological slices grouped according to the heart rhythm (SR vs. AF, ( C )); ( D ) TRPM7 and TRPM6 protein levels in human heart tissue homogenates grouped according to the heart rhythm and the presence of ischemic disease. Mean data of immunofluorescence provided in arbitrary units (a.u) for TRPM6 and TRPM7: * and ** p < 0.05 AF vs. SR, respectively, in isolated cells; # and ## p < 0.01 IHD vs. non-IHD, respectively, in tissue sections; $ and $$ p < 0.01 AF vs. SR, respectively, in tissue sections, and mean data of TRPM6 and TRPM7 protein concentrations in pg/mL, † and †† p < 0.05 AF vs. SR, ‡ and ‡‡ p < 0.05 IHD vs. SR (non-IHD), respectively.

    Journal: International Journal of Molecular Sciences

    Article Title: Detection of TRPM6 and TRPM7 Proteins in Normal and Diseased Cardiac Atrial Tissue and Isolated Cardiomyocytes

    doi: 10.3390/ijms232314860

    Figure Lengend Snippet: Effect of cardiac disease conditions on TRPM6 and TRPM7: ( A – C ) TRPM fluorescence intensity levels in RA cells ( A ) or tissues ( B , C ). Fluorescence intensity levels of TRPM6 (unfilled) and TRPM7 (filled) channels in isolated cardiomyocytes of the human heart, grouped according to the heart rhythm (SR vs. AF, ( A )) or according to the absence or presence of ischemic heart disease (non-IHD vs. IHD, ( B )). Fluorescence intensity levels of TRPM6 (unfilled) and TRPM7 (filled) channels in histological slices grouped according to the heart rhythm (SR vs. AF, ( C )); ( D ) TRPM7 and TRPM6 protein levels in human heart tissue homogenates grouped according to the heart rhythm and the presence of ischemic disease. Mean data of immunofluorescence provided in arbitrary units (a.u) for TRPM6 and TRPM7: * and ** p < 0.05 AF vs. SR, respectively, in isolated cells; # and ## p < 0.01 IHD vs. non-IHD, respectively, in tissue sections; $ and $$ p < 0.01 AF vs. SR, respectively, in tissue sections, and mean data of TRPM6 and TRPM7 protein concentrations in pg/mL, † and †† p < 0.05 AF vs. SR, ‡ and ‡‡ p < 0.05 IHD vs. SR (non-IHD), respectively.

    Article Snippet: The blocking peptide for anti-TRPM7 antibody (#BLP-CC047) and the blocking peptide for anti-TRPM6 (#BPL-CC046) was created by Alomone Labs for the antibody TRPM6 or TRPM7.

    Techniques: Fluorescence, Isolation, Immunofluorescence