black 96 well immuno plates  (Thermo Fisher)


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    Name:
    Black 96 Well Immuno Plates
    Description:
    Perform quantitative and qualitative immunoassays like ELISA and binding assays with Thermo Scientific Black 96 Well Immuno Plates featuring a choice of three surface types and optimal binding These plates minimize background in fluorescence reading
    Catalog Number:
    437111
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    None
    Applications:
    Clinical|Diagnostic Development
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    Structured Review

    Thermo Fisher black 96 well immuno plates
    Perform quantitative and qualitative immunoassays like ELISA and binding assays with Thermo Scientific Black 96 Well Immuno Plates featuring a choice of three surface types and optimal binding These plates minimize background in fluorescence reading
    https://www.bioz.com/result/black 96 well immuno plates/product/Thermo Fisher
    Average 99 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    black 96 well immuno plates - by Bioz Stars, 2020-04
    99/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Selecting antagonistic antibodies that control differentiation through inducible expression in embryonic stem cells
    Article Snippet: scFv gene inserts from stem cell genomic DNA of selected clones were subcloned into the NcoI/NotI-digested expression vector pBIOCAM5 and scFv-Fc expressed by transient transfection of HEK293F suspension cells ( ). .. Black Maxisorp 96-well plates (437111; Thermo Scientific Nunc) were coated with heparin sulfate proteoglycan (3 μg/mL in PBS; Sigma) overnight at 4 °C and blocked with PBS-M [2% (wt/vol) milk powder in PBS] for 1 h at room temperature.

    Centrifugation:

    Article Title: Macrophage Polarization Modulates FcγR- and CD13-Mediated Phagocytosis and Reactive Oxygen Species Production, Independently of Receptor Membrane Expression
    Article Snippet: To maximize contact, the cells mixed with the EBS-Fab were pelleted by a brief centrifugation. .. The cells were resuspended and transferred to wells of black 96-well Immuno Plates (Thermo Scientific, Waltham, MA, USA).

    Luciferase:

    Article Title: Identification and analysis of hepatitis C virus NS3 helicase inhibitors using nucleic acid binding assays
    Article Snippet: .. The effect of compounds on HCV replication was estimated by measuring the Renilla luciferase activity using the Renilla luciferase assay system (Promega, Madison, WI) in 96-well black microplates (Thermo Scientific, catalog #9502867) read on a FLUOstar Omega multi-mode microplate reader (BMG Labtech, Inc.). .. Relative percent inhibition was calculated by normalizing values to those obtained with cells treated with DMSO only.

    Article Title: Identification and analysis of hepatitis C virus NS3 helicase inhibitors using nucleic acid binding assays
    Article Snippet: .. The plate was incubated at 23°C for 30 min and luciferase activity was measured for 1 s using a FLUOstar Omega microplate reader (BMG Labtech, Inc.) in black 96-well microplates (Thermo Scientific, catalog #9502867). ..

    Positive Control:

    Article Title: Role of Sialidase in Mycoplasma alligatoris-induced Pulmonary Fibroblast Apoptosis
    Article Snippet: Sialidase activity was assayed quantitatively in 96-well plates (FluoroNunc 437112; Nalge Nunc International, Rochester NY) using the fluorogenic substrate 2′-(4-methylumbelliferyl)-α-D- N -acetylneuraminic acid (MUAN; Sigma-Aldrich, St. Louis MO) and a spectrofluorometer (SpectraMax M5 with SoftMax Pro 5 software; Molecular Devices, Sunnyvale CA). .. The positive control was Type VI Clostridium perfringens sialidase (Sigma-Aldrich).

    Construct:

    Article Title: Identification and analysis of hepatitis C virus NS3 helicase inhibitors using nucleic acid binding assays
    Article Snippet: HCV replicon assays The ability of compounds to inhibit HCV replication was judged using an HCV Renilla luciferase (HCV RLuc) reporter construct that was a generous gift from Seng-Lai Tan ( ). .. The effect of compounds on HCV replication was estimated by measuring the Renilla luciferase activity using the Renilla luciferase assay system (Promega, Madison, WI) in 96-well black microplates (Thermo Scientific, catalog #9502867) read on a FLUOstar Omega multi-mode microplate reader (BMG Labtech, Inc.).

    Enzyme-linked Immunosorbent Assay:

    Article Title: The IgH locus 3′ cis-regulatory super-enhancer co-opts AID for allelic transvection
    Article Snippet: .. For ELISA IgEa , black 96-well Immuno plates (Thermoscientific) were coated overnight with anti-IgE (732385, Beckman coulter). .. Samples were incubated 5h at 37°C and, after washing, with biotinylated anti-mouse IgEa (BLE408804, Ozyme) overnight at 37°C.

    Incubation:

    Article Title: Macrophage Polarization Modulates FcγR- and CD13-Mediated Phagocytosis and Reactive Oxygen Species Production, Independently of Receptor Membrane Expression
    Article Snippet: After washing, the receptors FcγRI, FcγRII, and CD13 were crosslinked for 30 min at 37°C by incubation with EBS-Fab at a ratio of 1 cell:20 EBS-Fab. .. The cells were resuspended and transferred to wells of black 96-well Immuno Plates (Thermo Scientific, Waltham, MA, USA).

    Article Title: Variable in Vivo and in Vitro Biological Effects of Cerium Oxide Nanoparticle Formulations
    Article Snippet: .. Briefly, for measuring catalase-mimetic activity, 60 µM CNRx, TI, or NP CeNPs were incubated with 10 µM H2 O2 in individual wells of black 96-well plates with clear optical bottoms (ThermoFisher Scientific; Grand Island, NY) for 30 minutes. .. Amplex® Red reagent mix was added to each well and incubated for another 30 minutes.

    Article Title: Identification and analysis of hepatitis C virus NS3 helicase inhibitors using nucleic acid binding assays
    Article Snippet: To each well, 0.5 µl of compounds dissolved in DMSO were added such that the DMSO final concentration was 0.5%, and the cells were incubated for 72 h at 37°C under 5% CO2 atmosphere. .. The effect of compounds on HCV replication was estimated by measuring the Renilla luciferase activity using the Renilla luciferase assay system (Promega, Madison, WI) in 96-well black microplates (Thermo Scientific, catalog #9502867) read on a FLUOstar Omega multi-mode microplate reader (BMG Labtech, Inc.).

    Article Title: The IgH locus 3′ cis-regulatory super-enhancer co-opts AID for allelic transvection
    Article Snippet: After washing AP-conjugates goat anti mouse IgA (1040-04, Southern biotech) or IgG2a (1080-04, Southern biotech) were incubated 1h at 37°C. .. For ELISA IgEa , black 96-well Immuno plates (Thermoscientific) were coated overnight with anti-IgE (732385, Beckman coulter).

    Article Title: Targeting cancer-specific glycans by cyclic peptide lectinomimics
    Article Snippet: Fluorescence-based assay was performed in black 96-well immuno plates (Thermo Scientific™ 437111). .. After washing of wells with PBS (2 x), immobilized BSA-monosaccharides were incubated for 1h with 10 µg/mL of AAL-FITC or 1 -FAM at room temperature in the dark, with gentle mixing on a shaker platform.

    Article Title: Identification and analysis of hepatitis C virus NS3 helicase inhibitors using nucleic acid binding assays
    Article Snippet: .. The plate was incubated at 23°C for 30 min and luciferase activity was measured for 1 s using a FLUOstar Omega microplate reader (BMG Labtech, Inc.) in black 96-well microplates (Thermo Scientific, catalog #9502867). ..

    Article Title: Membrane-bound and soluble forms of an NMDA receptor extracellular domain retain epitopes targeted in auto-immune encephalitis
    Article Snippet: ATD ELISAs To analyze the timecourse of ATD mobilization by TEV, we performed a capture assay in which we coated Black 96-well immune plates (12–566-24, Thermo Fisher) with 5 μg/mL anti HIS tag antibody (ab18184, Abcam, Cambridge, MA) overnight, washed the plates 3 times with PBST, blocked with 5% inactivated fetal bovine serum and 3% Goat serum (Invitrogen) in PBST for 1 h at 37 °C, then washed 3 times. .. ATD samples cleaved at the stated timepoints were added at 5 μg/mL and supernatant from un-cleaved cells was added as negative control, followed by 1 h incubation at 37 °C.

    Article Title: Membrane-bound and soluble forms of an NMDA receptor extracellular domain retain epitopes targeted in auto-immune encephalitis
    Article Snippet: To analyze the timecourse of ATD mobilization by TEV, we performed a capture assay in which we coated Black 96-well immune plates (12–566-24, Thermo Fisher) with 5 μg/mL anti HIS tag antibody (ab18184, Abcam, Cambridge, MA) overnight, washed the plates 3 times with PBST, blocked with 5% inactivated fetal bovine serum and 3% Goat serum (Invitrogen) in PBST for 1 h at 37 °C, then washed 3 times. .. ATD samples cleaved at the stated timepoints were added at 5 μg/mL and supernatant from un-cleaved cells was added as negative control, followed by 1 h incubation at 37 °C.

    Activity Assay:

    Article Title: Variable in Vivo and in Vitro Biological Effects of Cerium Oxide Nanoparticle Formulations
    Article Snippet: .. Briefly, for measuring catalase-mimetic activity, 60 µM CNRx, TI, or NP CeNPs were incubated with 10 µM H2 O2 in individual wells of black 96-well plates with clear optical bottoms (ThermoFisher Scientific; Grand Island, NY) for 30 minutes. .. Amplex® Red reagent mix was added to each well and incubated for another 30 minutes.

    Article Title: Identification and analysis of hepatitis C virus NS3 helicase inhibitors using nucleic acid binding assays
    Article Snippet: .. The effect of compounds on HCV replication was estimated by measuring the Renilla luciferase activity using the Renilla luciferase assay system (Promega, Madison, WI) in 96-well black microplates (Thermo Scientific, catalog #9502867) read on a FLUOstar Omega multi-mode microplate reader (BMG Labtech, Inc.). .. Relative percent inhibition was calculated by normalizing values to those obtained with cells treated with DMSO only.

    Article Title: The IgH locus 3′ cis-regulatory super-enhancer co-opts AID for allelic transvection
    Article Snippet: For ELISA IgEa , black 96-well Immuno plates (Thermoscientific) were coated overnight with anti-IgE (732385, Beckman coulter). .. After washing, AP activity was assayed using 4-methylumbelliferyl phosphate (Molecular probes) and fluorescence was read at 449 nm.

    Article Title: Identification and analysis of hepatitis C virus NS3 helicase inhibitors using nucleic acid binding assays
    Article Snippet: .. The plate was incubated at 23°C for 30 min and luciferase activity was measured for 1 s using a FLUOstar Omega microplate reader (BMG Labtech, Inc.) in black 96-well microplates (Thermo Scientific, catalog #9502867). ..

    Article Title: Role of Sialidase in Mycoplasma alligatoris-induced Pulmonary Fibroblast Apoptosis
    Article Snippet: .. Sialidase activity was assayed quantitatively in 96-well plates (FluoroNunc 437112; Nalge Nunc International, Rochester NY) using the fluorogenic substrate 2′-(4-methylumbelliferyl)-α-D- N -acetylneuraminic acid (MUAN; Sigma-Aldrich, St. Louis MO) and a spectrofluorometer (SpectraMax M5 with SoftMax Pro 5 software; Molecular Devices, Sunnyvale CA). ..

    Expressing:

    Article Title: Identification and analysis of hepatitis C virus NS3 helicase inhibitors using nucleic acid binding assays
    Article Snippet: Plasmid DNA expressing the replicon was transcribed, and the subsequently purified RNA was used to prepare stably transfected Huh7.5 cells by prolonged selection with G418. .. The effect of compounds on HCV replication was estimated by measuring the Renilla luciferase activity using the Renilla luciferase assay system (Promega, Madison, WI) in 96-well black microplates (Thermo Scientific, catalog #9502867) read on a FLUOstar Omega multi-mode microplate reader (BMG Labtech, Inc.).

    Article Title: Selecting antagonistic antibodies that control differentiation through inducible expression in embryonic stem cells
    Article Snippet: scFv gene inserts from stem cell genomic DNA of selected clones were subcloned into the NcoI/NotI-digested expression vector pBIOCAM5 and scFv-Fc expressed by transient transfection of HEK293F suspension cells ( ). .. Black Maxisorp 96-well plates (437111; Thermo Scientific Nunc) were coated with heparin sulfate proteoglycan (3 μg/mL in PBS; Sigma) overnight at 4 °C and blocked with PBS-M [2% (wt/vol) milk powder in PBS] for 1 h at room temperature.

    Transfection:

    Article Title: Identification and analysis of hepatitis C virus NS3 helicase inhibitors using nucleic acid binding assays
    Article Snippet: Plasmid DNA expressing the replicon was transcribed, and the subsequently purified RNA was used to prepare stably transfected Huh7.5 cells by prolonged selection with G418. .. The effect of compounds on HCV replication was estimated by measuring the Renilla luciferase activity using the Renilla luciferase assay system (Promega, Madison, WI) in 96-well black microplates (Thermo Scientific, catalog #9502867) read on a FLUOstar Omega multi-mode microplate reader (BMG Labtech, Inc.).

    Article Title: Selecting antagonistic antibodies that control differentiation through inducible expression in embryonic stem cells
    Article Snippet: scFv gene inserts from stem cell genomic DNA of selected clones were subcloned into the NcoI/NotI-digested expression vector pBIOCAM5 and scFv-Fc expressed by transient transfection of HEK293F suspension cells ( ). .. Black Maxisorp 96-well plates (437111; Thermo Scientific Nunc) were coated with heparin sulfate proteoglycan (3 μg/mL in PBS; Sigma) overnight at 4 °C and blocked with PBS-M [2% (wt/vol) milk powder in PBS] for 1 h at room temperature.

    Stable Transfection:

    Article Title: Identification and analysis of hepatitis C virus NS3 helicase inhibitors using nucleic acid binding assays
    Article Snippet: Plasmid DNA expressing the replicon was transcribed, and the subsequently purified RNA was used to prepare stably transfected Huh7.5 cells by prolonged selection with G418. .. The effect of compounds on HCV replication was estimated by measuring the Renilla luciferase activity using the Renilla luciferase assay system (Promega, Madison, WI) in 96-well black microplates (Thermo Scientific, catalog #9502867) read on a FLUOstar Omega multi-mode microplate reader (BMG Labtech, Inc.).

    Cell Culture:

    Article Title: Role of Sialidase in Mycoplasma alligatoris-induced Pulmonary Fibroblast Apoptosis
    Article Snippet: M. alligatoris strain A21JP2T was cultured as described ( ). .. Sialidase activity was assayed quantitatively in 96-well plates (FluoroNunc 437112; Nalge Nunc International, Rochester NY) using the fluorogenic substrate 2′-(4-methylumbelliferyl)-α-D- N -acetylneuraminic acid (MUAN; Sigma-Aldrich, St. Louis MO) and a spectrofluorometer (SpectraMax M5 with SoftMax Pro 5 software; Molecular Devices, Sunnyvale CA).

    Inhibition:

    Article Title: Identification and analysis of hepatitis C virus NS3 helicase inhibitors using nucleic acid binding assays
    Article Snippet: The effect of compounds on HCV replication was estimated by measuring the Renilla luciferase activity using the Renilla luciferase assay system (Promega, Madison, WI) in 96-well black microplates (Thermo Scientific, catalog #9502867) read on a FLUOstar Omega multi-mode microplate reader (BMG Labtech, Inc.). .. Relative percent inhibition was calculated by normalizing values to those obtained with cells treated with DMSO only.

    Imaging:

    Article Title: Macrophage Polarization Modulates FcγR- and CD13-Mediated Phagocytosis and Reactive Oxygen Species Production, Independently of Receptor Membrane Expression
    Article Snippet: The cells were resuspended and transferred to wells of black 96-well Immuno Plates (Thermo Scientific, Waltham, MA, USA). .. Fluorescence from oxidized carboxy-H2DFFDA was determined immediately (initial reading) and every 15 min thereafter, in a Cytation 3 Cell Imaging Multi-Mode Reader (BioTek, Winooski, VT, USA) for 60 min. For quantification of ROS production in M2 macrophages, cells were preincubated on ice with two different Fab fragments [anti-FcγRI (32.2) + anti-FcγRII (IV.3), or anti-FcγRI (32.2) + anti-CD13 (452)], or with three Fab fragments (32.2 + IV.3 + 452), or no treatment (No Fab).

    Affinity Purification:

    Article Title: Selecting antagonistic antibodies that control differentiation through inducible expression in embryonic stem cells
    Article Snippet: Affinity purification by Ni-NTA Sepharose was carried out as described previously ( ). .. Black Maxisorp 96-well plates (437111; Thermo Scientific Nunc) were coated with heparin sulfate proteoglycan (3 μg/mL in PBS; Sigma) overnight at 4 °C and blocked with PBS-M [2% (wt/vol) milk powder in PBS] for 1 h at room temperature.

    Binding Assay:

    Article Title: Selecting antagonistic antibodies that control differentiation through inducible expression in embryonic stem cells
    Article Snippet: Paragraph title: Development and Use of in Vitro Biochemical Assay Replicating Interaction of FGFR1 or FGFR2 Binding to FGF4. ... Black Maxisorp 96-well plates (437111; Thermo Scientific Nunc) were coated with heparin sulfate proteoglycan (3 μg/mL in PBS; Sigma) overnight at 4 °C and blocked with PBS-M [2% (wt/vol) milk powder in PBS] for 1 h at room temperature.

    Article Title: Targeting cancer-specific glycans by cyclic peptide lectinomimics
    Article Snippet: Paragraph title: Fluorescence-Based Binding Assay with BSA-Monosaccharides ... Fluorescence-based assay was performed in black 96-well immuno plates (Thermo Scientific™ 437111).

    Fluorescence:

    Article Title: Macrophage Polarization Modulates FcγR- and CD13-Mediated Phagocytosis and Reactive Oxygen Species Production, Independently of Receptor Membrane Expression
    Article Snippet: The cells were resuspended and transferred to wells of black 96-well Immuno Plates (Thermo Scientific, Waltham, MA, USA). .. Fluorescence from oxidized carboxy-H2DFFDA was determined immediately (initial reading) and every 15 min thereafter, in a Cytation 3 Cell Imaging Multi-Mode Reader (BioTek, Winooski, VT, USA) for 60 min. For quantification of ROS production in M2 macrophages, cells were preincubated on ice with two different Fab fragments [anti-FcγRI (32.2) + anti-FcγRII (IV.3), or anti-FcγRI (32.2) + anti-CD13 (452)], or with three Fab fragments (32.2 + IV.3 + 452), or no treatment (No Fab).

    Article Title: Variable in Vivo and in Vitro Biological Effects of Cerium Oxide Nanoparticle Formulations
    Article Snippet: Briefly, for measuring catalase-mimetic activity, 60 µM CNRx, TI, or NP CeNPs were incubated with 10 µM H2 O2 in individual wells of black 96-well plates with clear optical bottoms (ThermoFisher Scientific; Grand Island, NY) for 30 minutes. .. Hence, the level of fluorescence at the end of the experiment was inversely related to the catalase activity present in the sample.

    Article Title: The IgH locus 3′ cis-regulatory super-enhancer co-opts AID for allelic transvection
    Article Snippet: For ELISA IgEa , black 96-well Immuno plates (Thermoscientific) were coated overnight with anti-IgE (732385, Beckman coulter). .. After washing, AP activity was assayed using 4-methylumbelliferyl phosphate (Molecular probes) and fluorescence was read at 449 nm.

    Article Title: Targeting cancer-specific glycans by cyclic peptide lectinomimics
    Article Snippet: .. Fluorescence-based assay was performed in black 96-well immuno plates (Thermo Scientific™ 437111). .. A stock concentration of 1 mg/mL of each BSA-monosaccharide was prepared in DI water, and 50 µL were added into each well in 3 replicates and left to dry overnight overnight to inhibit potential adhesion of the tested lectin and peptide to the plastic surface and to assess the nonspecific interaction with BSA portion of the immobilized BSA-monosaccharides.

    Article Title: Role of Sialidase in Mycoplasma alligatoris-induced Pulmonary Fibroblast Apoptosis
    Article Snippet: Sialidase activity was assayed quantitatively in 96-well plates (FluoroNunc 437112; Nalge Nunc International, Rochester NY) using the fluorogenic substrate 2′-(4-methylumbelliferyl)-α-D- N -acetylneuraminic acid (MUAN; Sigma-Aldrich, St. Louis MO) and a spectrofluorometer (SpectraMax M5 with SoftMax Pro 5 software; Molecular Devices, Sunnyvale CA). .. The intensity of cyan fluorescence at 450 nm, excited at 365 nm with a cutoff filter at 420 nm, was proportional to sialidase activity ( ).

    Mouse Assay:

    Article Title: The IgH locus 3′ cis-regulatory super-enhancer co-opts AID for allelic transvection
    Article Snippet: Allotype-specific ELISA ELISA for the presence of “a” allotype IgAa , IgG2a, and IgEa were performed on sera or supernatant from Δ3′RR homozygous, Δ3′RRa /wt b heterozygous, wt a/b and wt b/b mice or in vitro stimulated B-cells. .. For ELISA IgEa , black 96-well Immuno plates (Thermoscientific) were coated overnight with anti-IgE (732385, Beckman coulter).

    Purification:

    Article Title: Identification and analysis of hepatitis C virus NS3 helicase inhibitors using nucleic acid binding assays
    Article Snippet: Plasmid DNA expressing the replicon was transcribed, and the subsequently purified RNA was used to prepare stably transfected Huh7.5 cells by prolonged selection with G418. .. The effect of compounds on HCV replication was estimated by measuring the Renilla luciferase activity using the Renilla luciferase assay system (Promega, Madison, WI) in 96-well black microplates (Thermo Scientific, catalog #9502867) read on a FLUOstar Omega multi-mode microplate reader (BMG Labtech, Inc.).

    Viability Assay:

    Article Title: Identification and analysis of hepatitis C virus NS3 helicase inhibitors using nucleic acid binding assays
    Article Snippet: Paragraph title: Cell viability assay ... The plate was incubated at 23°C for 30 min and luciferase activity was measured for 1 s using a FLUOstar Omega microplate reader (BMG Labtech, Inc.) in black 96-well microplates (Thermo Scientific, catalog #9502867).

    Plasmid Preparation:

    Article Title: Identification and analysis of hepatitis C virus NS3 helicase inhibitors using nucleic acid binding assays
    Article Snippet: Plasmid DNA expressing the replicon was transcribed, and the subsequently purified RNA was used to prepare stably transfected Huh7.5 cells by prolonged selection with G418. .. The effect of compounds on HCV replication was estimated by measuring the Renilla luciferase activity using the Renilla luciferase assay system (Promega, Madison, WI) in 96-well black microplates (Thermo Scientific, catalog #9502867) read on a FLUOstar Omega multi-mode microplate reader (BMG Labtech, Inc.).

    Article Title: Selecting antagonistic antibodies that control differentiation through inducible expression in embryonic stem cells
    Article Snippet: scFv gene inserts from stem cell genomic DNA of selected clones were subcloned into the NcoI/NotI-digested expression vector pBIOCAM5 and scFv-Fc expressed by transient transfection of HEK293F suspension cells ( ). .. Black Maxisorp 96-well plates (437111; Thermo Scientific Nunc) were coated with heparin sulfate proteoglycan (3 μg/mL in PBS; Sigma) overnight at 4 °C and blocked with PBS-M [2% (wt/vol) milk powder in PBS] for 1 h at room temperature.

    Software:

    Article Title: Role of Sialidase in Mycoplasma alligatoris-induced Pulmonary Fibroblast Apoptosis
    Article Snippet: .. Sialidase activity was assayed quantitatively in 96-well plates (FluoroNunc 437112; Nalge Nunc International, Rochester NY) using the fluorogenic substrate 2′-(4-methylumbelliferyl)-α-D- N -acetylneuraminic acid (MUAN; Sigma-Aldrich, St. Louis MO) and a spectrofluorometer (SpectraMax M5 with SoftMax Pro 5 software; Molecular Devices, Sunnyvale CA). ..

    Negative Control:

    Article Title: Membrane-bound and soluble forms of an NMDA receptor extracellular domain retain epitopes targeted in auto-immune encephalitis
    Article Snippet: ATD ELISAs To analyze the timecourse of ATD mobilization by TEV, we performed a capture assay in which we coated Black 96-well immune plates (12–566-24, Thermo Fisher) with 5 μg/mL anti HIS tag antibody (ab18184, Abcam, Cambridge, MA) overnight, washed the plates 3 times with PBST, blocked with 5% inactivated fetal bovine serum and 3% Goat serum (Invitrogen) in PBST for 1 h at 37 °C, then washed 3 times. .. ATD samples cleaved at the stated timepoints were added at 5 μg/mL and supernatant from un-cleaved cells was added as negative control, followed by 1 h incubation at 37 °C.

    Article Title: Membrane-bound and soluble forms of an NMDA receptor extracellular domain retain epitopes targeted in auto-immune encephalitis
    Article Snippet: To analyze the timecourse of ATD mobilization by TEV, we performed a capture assay in which we coated Black 96-well immune plates (12–566-24, Thermo Fisher) with 5 μg/mL anti HIS tag antibody (ab18184, Abcam, Cambridge, MA) overnight, washed the plates 3 times with PBST, blocked with 5% inactivated fetal bovine serum and 3% Goat serum (Invitrogen) in PBST for 1 h at 37 °C, then washed 3 times. .. ATD samples cleaved at the stated timepoints were added at 5 μg/mL and supernatant from un-cleaved cells was added as negative control, followed by 1 h incubation at 37 °C.

    Selection:

    Article Title: Identification and analysis of hepatitis C virus NS3 helicase inhibitors using nucleic acid binding assays
    Article Snippet: Plasmid DNA expressing the replicon was transcribed, and the subsequently purified RNA was used to prepare stably transfected Huh7.5 cells by prolonged selection with G418. .. The effect of compounds on HCV replication was estimated by measuring the Renilla luciferase activity using the Renilla luciferase assay system (Promega, Madison, WI) in 96-well black microplates (Thermo Scientific, catalog #9502867) read on a FLUOstar Omega multi-mode microplate reader (BMG Labtech, Inc.).

    In Vitro:

    Article Title: Selecting antagonistic antibodies that control differentiation through inducible expression in embryonic stem cells
    Article Snippet: Paragraph title: Development and Use of in Vitro Biochemical Assay Replicating Interaction of FGFR1 or FGFR2 Binding to FGF4. ... Black Maxisorp 96-well plates (437111; Thermo Scientific Nunc) were coated with heparin sulfate proteoglycan (3 μg/mL in PBS; Sigma) overnight at 4 °C and blocked with PBS-M [2% (wt/vol) milk powder in PBS] for 1 h at room temperature.

    Article Title: The IgH locus 3′ cis-regulatory super-enhancer co-opts AID for allelic transvection
    Article Snippet: Allotype-specific ELISA ELISA for the presence of “a” allotype IgAa , IgG2a, and IgEa were performed on sera or supernatant from Δ3′RR homozygous, Δ3′RRa /wt b heterozygous, wt a/b and wt b/b mice or in vitro stimulated B-cells. .. For ELISA IgEa , black 96-well Immuno plates (Thermoscientific) were coated overnight with anti-IgE (732385, Beckman coulter).

    Concentration Assay:

    Article Title: Identification and analysis of hepatitis C virus NS3 helicase inhibitors using nucleic acid binding assays
    Article Snippet: To each well, 0.5 µl of compounds dissolved in DMSO were added such that the DMSO final concentration was 0.5%, and the cells were incubated for 72 h at 37°C under 5% CO2 atmosphere. .. The effect of compounds on HCV replication was estimated by measuring the Renilla luciferase activity using the Renilla luciferase assay system (Promega, Madison, WI) in 96-well black microplates (Thermo Scientific, catalog #9502867) read on a FLUOstar Omega multi-mode microplate reader (BMG Labtech, Inc.).

    Article Title: Targeting cancer-specific glycans by cyclic peptide lectinomimics
    Article Snippet: Fluorescence-based assay was performed in black 96-well immuno plates (Thermo Scientific™ 437111). .. A stock concentration of 1 mg/mL of each BSA-monosaccharide was prepared in DI water, and 50 µL were added into each well in 3 replicates and left to dry overnight overnight to inhibit potential adhesion of the tested lectin and peptide to the plastic surface and to assess the nonspecific interaction with BSA portion of the immobilized BSA-monosaccharides.

    Lysis:

    Article Title: Identification and analysis of hepatitis C virus NS3 helicase inhibitors using nucleic acid binding assays
    Article Snippet: Briefly, at the end of a 72 h incubation period, the medium was removed and the cells were washed with growth medium, then an equal volume of growth medium and Cell Titer-Glo reagent was added and the lysis was initiated by mixing on an orbital shaker. .. The plate was incubated at 23°C for 30 min and luciferase activity was measured for 1 s using a FLUOstar Omega microplate reader (BMG Labtech, Inc.) in black 96-well microplates (Thermo Scientific, catalog #9502867).

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  • 99
    Thermo Fisher black 96 well immuno plates
    Black 96 Well Immuno Plates, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/black 96 well immuno plates/product/Thermo Fisher
    Average 99 stars, based on 20 article reviews
    Price from $9.99 to $1999.99
    black 96 well immuno plates - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

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    Thermo Fisher immuno standard modules
    Immuno Standard Modules, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/immuno standard modules/product/Thermo Fisher
    Average 99 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    immuno standard modules - by Bioz Stars, 2020-04
    99/100 stars
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