bl21∗de3 cells  (Millipore)


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    Structured Review

    Millipore bl21∗de3 cells
    Bl21∗De3 Cells, supplied by Millipore, used in various techniques. Bioz Stars score: 87/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bl21∗de3 cells/product/Millipore
    Average 87 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bl21∗de3 cells - by Bioz Stars, 2020-04
    87/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Cardiomyopathy-Related Mutations in Cardiac Troponin C, L29Q and G159D, Have Divergent Effects on Rat Cardiac Myofiber Contractile Dynamics
    Article Snippet: .. Expression and Purification of Recombinant Rat Cardiac Tn Subunits Recombinant c-myc tagged rat cardiac troponin T (c-myc RcTnT), troponin I (RcTnI), and troponin C (RcTnC), were all cloned into a pSBETa vector, and were expressed in BL21∗DE3 cells (Novagen, Madison, WI) for protein synthesis. .. L29Q and G159D substitutions in RcTnC were generated using site-directed DNA mutagenesis techniques and were cloned into a pSBETa vector.

    Ion Exchange Chromatography:

    Article Title: Cardiomyopathy-Related Mutations in Cardiac Troponin C, L29Q and G159D, Have Divergent Effects on Rat Cardiac Myofiber Contractile Dynamics
    Article Snippet: Expression and Purification of Recombinant Rat Cardiac Tn Subunits Recombinant c-myc tagged rat cardiac troponin T (c-myc RcTnT), troponin I (RcTnI), and troponin C (RcTnC), were all cloned into a pSBETa vector, and were expressed in BL21∗DE3 cells (Novagen, Madison, WI) for protein synthesis. .. BL21∗DE3 cells were lysed and the proteins (c-myc RcTnT, RcTnI, and RcTnC) were purified using ion-exchange chromatography techniques, as described previously [ , , ].

    Mutagenesis:

    Article Title: Cardiomyopathy-Related Mutations in Cardiac Troponin C, L29Q and G159D, Have Divergent Effects on Rat Cardiac Myofiber Contractile Dynamics
    Article Snippet: Expression and Purification of Recombinant Rat Cardiac Tn Subunits Recombinant c-myc tagged rat cardiac troponin T (c-myc RcTnT), troponin I (RcTnI), and troponin C (RcTnC), were all cloned into a pSBETa vector, and were expressed in BL21∗DE3 cells (Novagen, Madison, WI) for protein synthesis. .. L29Q and G159D substitutions in RcTnC were generated using site-directed DNA mutagenesis techniques and were cloned into a pSBETa vector.

    Purification:

    Article Title: Cardiomyopathy-Related Mutations in Cardiac Troponin C, L29Q and G159D, Have Divergent Effects on Rat Cardiac Myofiber Contractile Dynamics
    Article Snippet: .. Expression and Purification of Recombinant Rat Cardiac Tn Subunits Recombinant c-myc tagged rat cardiac troponin T (c-myc RcTnT), troponin I (RcTnI), and troponin C (RcTnC), were all cloned into a pSBETa vector, and were expressed in BL21∗DE3 cells (Novagen, Madison, WI) for protein synthesis. .. L29Q and G159D substitutions in RcTnC were generated using site-directed DNA mutagenesis techniques and were cloned into a pSBETa vector.

    Generated:

    Article Title: Cardiomyopathy-Related Mutations in Cardiac Troponin C, L29Q and G159D, Have Divergent Effects on Rat Cardiac Myofiber Contractile Dynamics
    Article Snippet: Expression and Purification of Recombinant Rat Cardiac Tn Subunits Recombinant c-myc tagged rat cardiac troponin T (c-myc RcTnT), troponin I (RcTnI), and troponin C (RcTnC), were all cloned into a pSBETa vector, and were expressed in BL21∗DE3 cells (Novagen, Madison, WI) for protein synthesis. .. L29Q and G159D substitutions in RcTnC were generated using site-directed DNA mutagenesis techniques and were cloned into a pSBETa vector.

    Expressing:

    Article Title: Cardiomyopathy-Related Mutations in Cardiac Troponin C, L29Q and G159D, Have Divergent Effects on Rat Cardiac Myofiber Contractile Dynamics
    Article Snippet: .. Expression and Purification of Recombinant Rat Cardiac Tn Subunits Recombinant c-myc tagged rat cardiac troponin T (c-myc RcTnT), troponin I (RcTnI), and troponin C (RcTnC), were all cloned into a pSBETa vector, and were expressed in BL21∗DE3 cells (Novagen, Madison, WI) for protein synthesis. .. L29Q and G159D substitutions in RcTnC were generated using site-directed DNA mutagenesis techniques and were cloned into a pSBETa vector.

    Sonication:

    Article Title: Cardiomyopathy-Related Mutations in Cardiac Troponin C, L29Q and G159D, Have Divergent Effects on Rat Cardiac Myofiber Contractile Dynamics
    Article Snippet: Expression and Purification of Recombinant Rat Cardiac Tn Subunits Recombinant c-myc tagged rat cardiac troponin T (c-myc RcTnT), troponin I (RcTnI), and troponin C (RcTnC), were all cloned into a pSBETa vector, and were expressed in BL21∗DE3 cells (Novagen, Madison, WI) for protein synthesis. .. In brief, BL21∗DE3 cells of each protein preparation (~4 liters) were spun down and sonicated in 50 mM Tris (pH 8.0 at 4°C), 6 M urea, 5 mM EDTA, 0.2 mM PMSF, 5 mM benzamidine-HCl, 10 mM leupeptin, 1 mM pepstatin, 5 mM bestatin, 2 mM E-64, and 1 mM DTT.

    Recombinant:

    Article Title: Cardiomyopathy-Related Mutations in Cardiac Troponin C, L29Q and G159D, Have Divergent Effects on Rat Cardiac Myofiber Contractile Dynamics
    Article Snippet: .. Expression and Purification of Recombinant Rat Cardiac Tn Subunits Recombinant c-myc tagged rat cardiac troponin T (c-myc RcTnT), troponin I (RcTnI), and troponin C (RcTnC), were all cloned into a pSBETa vector, and were expressed in BL21∗DE3 cells (Novagen, Madison, WI) for protein synthesis. .. L29Q and G159D substitutions in RcTnC were generated using site-directed DNA mutagenesis techniques and were cloned into a pSBETa vector.

    Plasmid Preparation:

    Article Title: Cardiomyopathy-Related Mutations in Cardiac Troponin C, L29Q and G159D, Have Divergent Effects on Rat Cardiac Myofiber Contractile Dynamics
    Article Snippet: .. Expression and Purification of Recombinant Rat Cardiac Tn Subunits Recombinant c-myc tagged rat cardiac troponin T (c-myc RcTnT), troponin I (RcTnI), and troponin C (RcTnC), were all cloned into a pSBETa vector, and were expressed in BL21∗DE3 cells (Novagen, Madison, WI) for protein synthesis. .. L29Q and G159D substitutions in RcTnC were generated using site-directed DNA mutagenesis techniques and were cloned into a pSBETa vector.

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