bl21 startm de3  (Thermo Fisher)


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    Structured Review

    Thermo Fisher bl21 startm de3
    Bl21 Startm De3, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bl21 startm de3/product/Thermo Fisher
    Average 88 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    bl21 startm de3 - by Bioz Stars, 2020-09
    88/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Insights into an evolutionary strategy leading to antibiotic resistance
    Article Snippet: .. The resulting PCR products were cloned into the pET26b(+) vector, and the constructs were then transferred into BL21 StarTM(DE3) (Invitrogen, California, USA). .. Subsequently, the transformed cells were grown on Mueller-Hinton agar plates, supplemented with 50 μg/mL kanamycin and 50–125 μg/mL cefoxitin for selection.

    In Vivo:

    Article Title: Towards characterization of DNA structure under physiological conditions in vivo at the single-molecule level using single-pair FRET
    Article Snippet: .. Incorporation of DNA into E. coli cells The BL21 StarTM(DE3)plysS One Shot (Invitrogen, US) strain of E. coli was used for all in vivo FRET measurements. .. The DNA constructs were introduced into E. coli cells using heat shock with an optimized protocol allowing the introduction of one to five DNA molecules per cell (for details see Supplementary Data ).

    Construct:

    Article Title: Insights into an evolutionary strategy leading to antibiotic resistance
    Article Snippet: .. The resulting PCR products were cloned into the pET26b(+) vector, and the constructs were then transferred into BL21 StarTM(DE3) (Invitrogen, California, USA). .. Subsequently, the transformed cells were grown on Mueller-Hinton agar plates, supplemented with 50 μg/mL kanamycin and 50–125 μg/mL cefoxitin for selection.

    Article Title: Interacting Proteins on Human Spermatozoa: Adaptive Evolution of the Binding of Semenogelin I to EPPIN
    Article Snippet: .. Recombinant proteins were expressed either in BL21 StarTM (DE3) One Shot® E. coli (Invitrogen; SEMG1 constructs) or E. coli Rosetta-Gami 2TM (DE3) (Novagen, Madison, WI; EPPIN construct) and purified under denaturing conditions using nickel-nitrilotriacetic acid agarose (Ni-NTA) beads (Qiagen, Valencia, CA) as described previously [ ]. .. Removal of N-terminal 6X His-tag from recombinant EPPIN was performed using recombinant enterokinase cleavage/capture kit (Novagen), according to the manufacturer’s instructions.

    Purification:

    Article Title: Interacting Proteins on Human Spermatozoa: Adaptive Evolution of the Binding of Semenogelin I to EPPIN
    Article Snippet: .. Recombinant proteins were expressed either in BL21 StarTM (DE3) One Shot® E. coli (Invitrogen; SEMG1 constructs) or E. coli Rosetta-Gami 2TM (DE3) (Novagen, Madison, WI; EPPIN construct) and purified under denaturing conditions using nickel-nitrilotriacetic acid agarose (Ni-NTA) beads (Qiagen, Valencia, CA) as described previously [ ]. .. Removal of N-terminal 6X His-tag from recombinant EPPIN was performed using recombinant enterokinase cleavage/capture kit (Novagen), according to the manufacturer’s instructions.

    Article Title: Kif2C Minimal Functional Domain Has Unusual Nucleotide Binding Properties That Are Adapted to Microtubule Depolymerization *
    Article Snippet: .. All the recombinant Kif2C fragments were expressed in BL21 StarTM (DE3) (Invitrogen) and purified on a HisTrap FF column followed by a Mono S column (GE Healthcare). .. More than 10 mg of pure recombinant protein was routinely obtained from a 1-liter Escherichia coli culture.

    Concentration Assay:

    Article Title: Characterization of Conformation-dependent Prion Protein Epitopes *
    Article Snippet: .. Transformed BL21 StarTM (DE3) (Invitrogen) were cultured until the absorbance value reached 1.0 at 650 nm and induced using isopropyl 1-thio-β- d -galactopyranoside (Sigma) at a final concentration of 1 m m . .. Bacterial pellets were resuspended and lysed with CelLytic solution (Sigma) and centrifuged to obtain inclusion bodies, following solubilization with CelLytic IB solution (Sigma).

    Cell Culture:

    Article Title: Characterization of Conformation-dependent Prion Protein Epitopes *
    Article Snippet: .. Transformed BL21 StarTM (DE3) (Invitrogen) were cultured until the absorbance value reached 1.0 at 650 nm and induced using isopropyl 1-thio-β- d -galactopyranoside (Sigma) at a final concentration of 1 m m . .. Bacterial pellets were resuspended and lysed with CelLytic solution (Sigma) and centrifuged to obtain inclusion bodies, following solubilization with CelLytic IB solution (Sigma).

    Expressing:

    Article Title: Identification of Three Monofunctional Diterpene Synthases with Specific Enzyme Activities Expressed during Heartwood Formation in Western Redcedar (Thuja plicata) Trees
    Article Snippet: .. For the expression of putative terpene synthase genes, plasmid carrying the gene of interest together with individual pGG, pGGeC, pGGsC, pGGnC plasmids was sequentially transformed into BL21 StarTM (DE3, ThermoFisher, Waltham, USA) chemically competent E. coli cells. .. To confirm the stereochemistry of normal-CPS activity, TpdiTPS3 was co-expressed with pGG and a class I labdane related diterpene synthase, i.e., Arabidopsis thaliana kaurene synthase (AtKS) that only reacts with ent -CPP.

    Article Title: A Versatile Strategy for Production of Membrane Proteins with Diverse Topologies: Application to Investigation of Bacterial Homologues of Human Divalent Metal Ion and Nucleoside Transporters
    Article Snippet: .. Expression trials Expression trials were typically performed in four E . coli strains in parallel: BL21-gold(DE3) (Stratagene), BL21 StarTM (DE3) (Invitrogen; [ ]), C41(DE3) and C43(DE3) (Lucigen Corporation; [ ]), all of which had been transformed with the plasmid pRARE2 (Novagen) to enhance translation efficiency of ORFs with codon usage different from endogenous E . coli genes [ ]. .. Typically, autoinduction of 1 mL cultures in modified M9 medium (M9auto ); Lysogeny broth (LBauto ) or Superbroth medium (SBauto ) containing 0.5% glycerol, 0.05% glucose and 0.2% lactose was performed at 37°C for 24 h in 24-deep-well plates as previously described [ ].

    Polymerase Chain Reaction:

    Article Title: Insights into an evolutionary strategy leading to antibiotic resistance
    Article Snippet: .. The resulting PCR products were cloned into the pET26b(+) vector, and the constructs were then transferred into BL21 StarTM(DE3) (Invitrogen, California, USA). .. Subsequently, the transformed cells were grown on Mueller-Hinton agar plates, supplemented with 50 μg/mL kanamycin and 50–125 μg/mL cefoxitin for selection.

    Transformation Assay:

    Article Title: Protein Methionine Sulfoxide Dynamics in Arabidopsis thaliana
    Article Snippet: .. BL21 StarTM One Shot chemically competent Escherichia coli cells (Invitrogen) were transformed, and GSTF9 and GSTT23 protein production was induced with 0.2 m m isopropyl β- d -1-thiogalactopyranoside in growing cultures of successfully transformed E. coli cells reaching an A 600 of 0.8. .. After incubation overnight at 16 °C, cells were centrifuged for 10 min at 2700 × g and resuspended in 20 ml of cold PBS with 1 m m DTT, 1% Triton X-100, and the appropriate amount of Complete protease inhibitor mixture.

    Article Title: Characterization of Conformation-dependent Prion Protein Epitopes *
    Article Snippet: .. Transformed BL21 StarTM (DE3) (Invitrogen) were cultured until the absorbance value reached 1.0 at 650 nm and induced using isopropyl 1-thio-β- d -galactopyranoside (Sigma) at a final concentration of 1 m m . .. Bacterial pellets were resuspended and lysed with CelLytic solution (Sigma) and centrifuged to obtain inclusion bodies, following solubilization with CelLytic IB solution (Sigma).

    Article Title: Identification of Three Monofunctional Diterpene Synthases with Specific Enzyme Activities Expressed during Heartwood Formation in Western Redcedar (Thuja plicata) Trees
    Article Snippet: .. For the expression of putative terpene synthase genes, plasmid carrying the gene of interest together with individual pGG, pGGeC, pGGsC, pGGnC plasmids was sequentially transformed into BL21 StarTM (DE3, ThermoFisher, Waltham, USA) chemically competent E. coli cells. .. To confirm the stereochemistry of normal-CPS activity, TpdiTPS3 was co-expressed with pGG and a class I labdane related diterpene synthase, i.e., Arabidopsis thaliana kaurene synthase (AtKS) that only reacts with ent -CPP.

    Article Title: A Versatile Strategy for Production of Membrane Proteins with Diverse Topologies: Application to Investigation of Bacterial Homologues of Human Divalent Metal Ion and Nucleoside Transporters
    Article Snippet: .. Expression trials Expression trials were typically performed in four E . coli strains in parallel: BL21-gold(DE3) (Stratagene), BL21 StarTM (DE3) (Invitrogen; [ ]), C41(DE3) and C43(DE3) (Lucigen Corporation; [ ]), all of which had been transformed with the plasmid pRARE2 (Novagen) to enhance translation efficiency of ORFs with codon usage different from endogenous E . coli genes [ ]. .. Typically, autoinduction of 1 mL cultures in modified M9 medium (M9auto ); Lysogeny broth (LBauto ) or Superbroth medium (SBauto ) containing 0.5% glycerol, 0.05% glucose and 0.2% lactose was performed at 37°C for 24 h in 24-deep-well plates as previously described [ ].

    Recombinant:

    Article Title: Interacting Proteins on Human Spermatozoa: Adaptive Evolution of the Binding of Semenogelin I to EPPIN
    Article Snippet: .. Recombinant proteins were expressed either in BL21 StarTM (DE3) One Shot® E. coli (Invitrogen; SEMG1 constructs) or E. coli Rosetta-Gami 2TM (DE3) (Novagen, Madison, WI; EPPIN construct) and purified under denaturing conditions using nickel-nitrilotriacetic acid agarose (Ni-NTA) beads (Qiagen, Valencia, CA) as described previously [ ]. .. Removal of N-terminal 6X His-tag from recombinant EPPIN was performed using recombinant enterokinase cleavage/capture kit (Novagen), according to the manufacturer’s instructions.

    Article Title: Kif2C Minimal Functional Domain Has Unusual Nucleotide Binding Properties That Are Adapted to Microtubule Depolymerization *
    Article Snippet: .. All the recombinant Kif2C fragments were expressed in BL21 StarTM (DE3) (Invitrogen) and purified on a HisTrap FF column followed by a Mono S column (GE Healthcare). .. More than 10 mg of pure recombinant protein was routinely obtained from a 1-liter Escherichia coli culture.

    Plasmid Preparation:

    Article Title: Insights into an evolutionary strategy leading to antibiotic resistance
    Article Snippet: .. The resulting PCR products were cloned into the pET26b(+) vector, and the constructs were then transferred into BL21 StarTM(DE3) (Invitrogen, California, USA). .. Subsequently, the transformed cells were grown on Mueller-Hinton agar plates, supplemented with 50 μg/mL kanamycin and 50–125 μg/mL cefoxitin for selection.

    Article Title: Identification of Three Monofunctional Diterpene Synthases with Specific Enzyme Activities Expressed during Heartwood Formation in Western Redcedar (Thuja plicata) Trees
    Article Snippet: .. For the expression of putative terpene synthase genes, plasmid carrying the gene of interest together with individual pGG, pGGeC, pGGsC, pGGnC plasmids was sequentially transformed into BL21 StarTM (DE3, ThermoFisher, Waltham, USA) chemically competent E. coli cells. .. To confirm the stereochemistry of normal-CPS activity, TpdiTPS3 was co-expressed with pGG and a class I labdane related diterpene synthase, i.e., Arabidopsis thaliana kaurene synthase (AtKS) that only reacts with ent -CPP.

    Article Title: A Versatile Strategy for Production of Membrane Proteins with Diverse Topologies: Application to Investigation of Bacterial Homologues of Human Divalent Metal Ion and Nucleoside Transporters
    Article Snippet: .. Expression trials Expression trials were typically performed in four E . coli strains in parallel: BL21-gold(DE3) (Stratagene), BL21 StarTM (DE3) (Invitrogen; [ ]), C41(DE3) and C43(DE3) (Lucigen Corporation; [ ]), all of which had been transformed with the plasmid pRARE2 (Novagen) to enhance translation efficiency of ORFs with codon usage different from endogenous E . coli genes [ ]. .. Typically, autoinduction of 1 mL cultures in modified M9 medium (M9auto ); Lysogeny broth (LBauto ) or Superbroth medium (SBauto ) containing 0.5% glycerol, 0.05% glucose and 0.2% lactose was performed at 37°C for 24 h in 24-deep-well plates as previously described [ ].

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  • 99
    Thermo Fisher bl21 startm de3 e coli cells
    Bl21 Startm De3 E Coli Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bl21 startm de3 e coli cells/product/Thermo Fisher
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    bl21 startm de3 e coli cells - by Bioz Stars, 2020-09
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      Buy from Supplier

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    Thermo Fisher bl21 startm
    Bl21 Startm, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bl21 startm/product/Thermo Fisher
    Average 88 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    bl21 startm - by Bioz Stars, 2020-09
    88/100 stars
      Buy from Supplier

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    Thermo Fisher e coli bl21 startm de3 f ompt hsdsb f ompt hsdsb rb mb gal dcm rne 131 λ
    E Coli Bl21 Startm De3 F Ompt Hsdsb F Ompt Hsdsb Rb Mb Gal Dcm Rne 131 λ, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e coli bl21 startm de3 f ompt hsdsb f ompt hsdsb rb mb gal dcm rne 131 λ/product/Thermo Fisher
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    e coli bl21 startm de3 f ompt hsdsb f ompt hsdsb rb mb gal dcm rne 131 λ - by Bioz Stars, 2020-09
    91/100 stars
      Buy from Supplier

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