bl21 star de3 plyss  (Thermo Fisher)


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    Name:
    BL21 Star DE3 pLysS One Shot Chemically Competent E coli
    Description:
    One Shot BL21 Star DE3 pLysS E coli are chemically competent cells designed for applications that require high level expression of non toxic recombinant proteins from high copy number T7 promoter based expression systems e g pRSET T7 vectors One Shot BL21 Star DE3 pLysS Chemically Competent cells have a transformation efficiency of 1 x 108 cfu µg plasmid DNA • High mRNA stability results in increased protein yield• Ideal for use with high copy number T7 promoter based plasmids• Low background expression in uninduced cellsIncreased mRNA Stability Improves Protein Yield One Shot BL21 Star DE3 pLysS E coli offers enhanced mRNA stability so that abundant mRNA is available for protein expression This enhanced stability is due to a mutation in the RNaseE gene rne131 which is involved in mRNA degradation High Expression of Non Toxic Recombinant Proteins One Shot BL21 Star DE3 pLysS cells are ideal for high level expression of non toxic but potentially growth inhibiting recombinant proteins from high copy number T7 promoter based expression systems The pLysS CamR plasmid carried by the BL21 Star DE3 pLysS strain expresses T7 lysozyme a T7 RNA polymerase inhibitor that prevents leaky expression in uninduced cells The p15a origin of pLysS makes this plasmid compatible with pUC or pBR322 derived plasmids This strain also contains the DE3 lysogen that carries the T7 RNA polymerase gene under control of the lacUV5 promoter and IPTG is required to induce expression of the T7 RNA polymerase One Shot BL21 Star DE3 pLysS cells do not contain the lon protease and are also deficient in the outer membrane protease OmpT The absence of these proteases reduces degradation of heterologous proteins Note The BL21 Star strains have higher basal expression of heterologous genes than BL21 strains due to the increased stability of mRNA Therefore these strains may not be useful for expression of toxic genes However the BL21 Star DE3 pLysS strains offer lower expression levels than the BL21 Star strains Genotype F ompT hsdSB rB mB galdcmrne131 DE3 pLysS CamR Easy to use Single Tube Format The single tube single use format allows for all steps of transformation up to plating to take place in the same tube thereby helping to save time and prevent contamination Find the Strain and Format That You Need We also offer many other different strains and formats of chemically competent cells and electrocompetent cells to help meet your specific transformation needs For expression of toxic proteins choose BL21 AI One Shot Chemically Competent E coli For expression of non toxic recombinant proteins from low copy number e g Champion pET vectors T7 promoter based expression system choose our One Shot BL21 Star DE3 chemically competent cells For Research Use Only Not intended for animal or human diagnostic or therapeutic use
    Catalog Number:
    c602003
    Price:
    None
    Applications:
    Bacterial Expression|Bioproduction|Competent Cells for Bacterial Expression|Protein Biology|Protein Expression
    Category:
    Competent Cells Strains
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    Structured Review

    Thermo Fisher bl21 star de3 plyss
    One Shot BL21 Star DE3 pLysS E coli are chemically competent cells designed for applications that require high level expression of non toxic recombinant proteins from high copy number T7 promoter based expression systems e g pRSET T7 vectors One Shot BL21 Star DE3 pLysS Chemically Competent cells have a transformation efficiency of 1 x 108 cfu µg plasmid DNA • High mRNA stability results in increased protein yield• Ideal for use with high copy number T7 promoter based plasmids• Low background expression in uninduced cellsIncreased mRNA Stability Improves Protein Yield One Shot BL21 Star DE3 pLysS E coli offers enhanced mRNA stability so that abundant mRNA is available for protein expression This enhanced stability is due to a mutation in the RNaseE gene rne131 which is involved in mRNA degradation High Expression of Non Toxic Recombinant Proteins One Shot BL21 Star DE3 pLysS cells are ideal for high level expression of non toxic but potentially growth inhibiting recombinant proteins from high copy number T7 promoter based expression systems The pLysS CamR plasmid carried by the BL21 Star DE3 pLysS strain expresses T7 lysozyme a T7 RNA polymerase inhibitor that prevents leaky expression in uninduced cells The p15a origin of pLysS makes this plasmid compatible with pUC or pBR322 derived plasmids This strain also contains the DE3 lysogen that carries the T7 RNA polymerase gene under control of the lacUV5 promoter and IPTG is required to induce expression of the T7 RNA polymerase One Shot BL21 Star DE3 pLysS cells do not contain the lon protease and are also deficient in the outer membrane protease OmpT The absence of these proteases reduces degradation of heterologous proteins Note The BL21 Star strains have higher basal expression of heterologous genes than BL21 strains due to the increased stability of mRNA Therefore these strains may not be useful for expression of toxic genes However the BL21 Star DE3 pLysS strains offer lower expression levels than the BL21 Star strains Genotype F ompT hsdSB rB mB galdcmrne131 DE3 pLysS CamR Easy to use Single Tube Format The single tube single use format allows for all steps of transformation up to plating to take place in the same tube thereby helping to save time and prevent contamination Find the Strain and Format That You Need We also offer many other different strains and formats of chemically competent cells and electrocompetent cells to help meet your specific transformation needs For expression of toxic proteins choose BL21 AI One Shot Chemically Competent E coli For expression of non toxic recombinant proteins from low copy number e g Champion pET vectors T7 promoter based expression system choose our One Shot BL21 Star DE3 chemically competent cells For Research Use Only Not intended for animal or human diagnostic or therapeutic use
    https://www.bioz.com/result/bl21 star de3 plyss/product/Thermo Fisher
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    bl21 star de3 plyss - by Bioz Stars, 2020-09
    99/100 stars

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    Related Articles

    In Vivo:

    Article Title: Towards characterization of DNA structure under physiological conditions in vivo at the single-molecule level using single-pair FRET
    Article Snippet: .. Incorporation of DNA into E. coli cells The BL21 StarTM(DE3)plysS One Shot (Invitrogen, US) strain of E. coli was used for all in vivo FRET measurements. .. The DNA constructs were introduced into E. coli cells using heat shock with an optimized protocol allowing the introduction of one to five DNA molecules per cell (for details see Supplementary Data ).

    Positron Emission Tomography:

    Article Title: Inhibition of the NKp44-PCNA Immune Checkpoint Using a mAb to PCNA
    Article Snippet: .. Recombinant human PCNA productionThe pET-28 and pMAL-c2x vectors were used to produce soluble human His-tagged and MBP-tagged PCNA respectively in Rosetta™ 2 (DE3) cells. .. Plasmids containing the cDNA or coding sequence of PCNA were transformed into Rosetta™ 2 cells via heat shock, and grown on LB agar plates with kanamycin and chloramphenicol selection.

    Mutagenesis:

    Article Title: Expression, purification and proteomic analysis of recombinant histone H4 acetylated at lysine 16
    Article Snippet: .. Switching to an RNaseE mutant BL21 strain (Invitrogen #C6020-03) substantially improved the expressed protein yield (approximately 200 ug per liter of culture) and allowed its visualization in Coomassie-stained gels ( ). ..

    Construct:

    Article Title: ALS-linked mutations affect UBQLN2 oligomerization and phase separation in a position- and amino acid-dependent manner
    Article Snippet: .. Briefly, the constructs were expressed in Escherichia coli Rosetta 2 (DE3) pLysS cells in Luria-Bertani (LB) broth at 37°C overnight. .. Briefly, NaCl was added to the cleared lysate to the final concentration of 0.5 M-1 M. UBQLN2 droplets were pelleted and then resuspended in 20 mM NaPhosphate, 0.5 mM EDTA (pH 6.8).

    Purification:

    Article Title: Design of a Human Rhinovirus-14 3C Protease-Inducible Caspase-3
    Article Snippet: .. Protein Production and Purification All proteins were produced in BL21(DE3)pLysS E. coli (Thermo Fisher Scientific, Waltham, MA, USA, # C602003). ..

    Produced:

    Article Title: A Two-Step Approach for the Design and Generation of Nanobodies
    Article Snippet: .. Strep-tag-BoNT/A-LC was produced in E. coli BL21(DE3) pLysS (Thermo Fisher Scientific, Waltham, MA, USA, # C602003). ..

    Article Title: Design of a Human Rhinovirus-14 3C Protease-Inducible Caspase-3
    Article Snippet: .. Protein Production and Purification All proteins were produced in BL21(DE3)pLysS E. coli (Thermo Fisher Scientific, Waltham, MA, USA, # C602003). ..

    Transformation Assay:

    Article Title: Allele-specific binding of RNA-binding proteins reveals functional genetic variants in the RNA
    Article Snippet: .. It was transformed into BL21 Star (DE3)-competent cells (Thermo Fisher Scientific, C602003). .. Protein induction was carried out via 1 mM isopropyl β-d -1-thiogalactopyranoside (IPTG) treatment in 50 mL cultured cells (OD = 0.8) for 16 h at 215 rpm at 28 °C.

    Recombinant:

    Article Title: Inhibition of the NKp44-PCNA Immune Checkpoint Using a mAb to PCNA
    Article Snippet: .. Recombinant human PCNA productionThe pET-28 and pMAL-c2x vectors were used to produce soluble human His-tagged and MBP-tagged PCNA respectively in Rosetta™ 2 (DE3) cells. .. Plasmids containing the cDNA or coding sequence of PCNA were transformed into Rosetta™ 2 cells via heat shock, and grown on LB agar plates with kanamycin and chloramphenicol selection.

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    Thermo Fisher bl21 star de3 plyss one shot chemically competent e coli
    Bl21 Star De3 Plyss One Shot Chemically Competent E Coli, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bl21 star de3 plyss one shot chemically competent e coli/product/Thermo Fisher
    Average 99 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    bl21 star de3 plyss one shot chemically competent e coli - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher one shot bl21 star
    One Shot Bl21 Star, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/one shot bl21 star/product/Thermo Fisher
    Average 99 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    one shot bl21 star - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

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