bl21 star de3 plyss  (Thermo Fisher)


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    Thermo Fisher bl21 star de3 plyss
    Bl21 Star De3 Plyss, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bl21 star de3 plyss/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bl21 star de3 plyss - by Bioz Stars, 2020-02
    86/100 stars

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    Related Articles

    Clone Assay:

    Article Title: TraG Encoded by the pIP501 Type IV Secretion System Is a Two-Domain Peptidoglycan-Degrading Enzyme Essential for Conjugative Transfer
    Article Snippet: The ligation mixtures were transformed into E. coli DH5α, Top10 (Life Technologies), XL10 Gold (Promega), and BL21 Star-pLysS (Life Technologies), respectively, and incubated at 37°C. .. The cloning experiment with the traG wild-type gene cloned into pEU327 was repeated applying E. coli DH5α and One Shot Mach1-T1R chemically competent E. coli cells (Invitrogen) as host and testing distinct incubation temperatures for the selective plates, namely, room temperature, 30°C, and 37°C.

    Centrifugation:

    Article Title: G-quadruplex recognition activities of E. Coli MutS
    Article Snippet: Sequence verified pTX412 and mutant derivatives were transformed into BL21 Star (DE3) pLysS (Invitrogen) and purifications were performed from over-expressed cultures typically at the 0.5 L scale. .. Cells were collected by centrifugation, and HIS-tagged proteins were purified by Nickel chromatography (Sigma, St. Louis, MO) as described previously [ ].

    Binding Assay:

    Article Title: TraG Encoded by the pIP501 Type IV Secretion System Is a Two-Domain Peptidoglycan-Degrading Enzyme Essential for Conjugative Transfer
    Article Snippet: To amplify traG with its own ribosomal binding site (RBS), TraG_RBS_SalI-fw and TraG_Sal1-rev primers were used (see Table S1 in the supplemental material). .. The ligation mixtures were transformed into E. coli DH5α, Top10 (Life Technologies), XL10 Gold (Promega), and BL21 Star-pLysS (Life Technologies), respectively, and incubated at 37°C.

    Chromatography:

    Article Title: G-quadruplex recognition activities of E. Coli MutS
    Article Snippet: Sequence verified pTX412 and mutant derivatives were transformed into BL21 Star (DE3) pLysS (Invitrogen) and purifications were performed from over-expressed cultures typically at the 0.5 L scale. .. Cells were collected by centrifugation, and HIS-tagged proteins were purified by Nickel chromatography (Sigma, St. Louis, MO) as described previously [ ].

    Ligation:

    Article Title: TraG Encoded by the pIP501 Type IV Secretion System Is a Two-Domain Peptidoglycan-Degrading Enzyme Essential for Conjugative Transfer
    Article Snippet: .. The ligation mixtures were transformed into E. coli DH5α, Top10 (Life Technologies), XL10 Gold (Promega), and BL21 Star-pLysS (Life Technologies), respectively, and incubated at 37°C. ..

    Mutagenesis:

    Article Title: G-quadruplex recognition activities of E. Coli MutS
    Article Snippet: .. Sequence verified pTX412 and mutant derivatives were transformed into BL21 Star (DE3) pLysS (Invitrogen) and purifications were performed from over-expressed cultures typically at the 0.5 L scale. ..

    Article Title: Fine Tuning of Spatial Arrangement of Enzymes in a PCNA-Mediated Multienzyme Complex Using a Rigid Poly-L-Proline Linker
    Article Snippet: Preparation of PUPPETs PCNA1-PdR and PCNA2-PdX fusion proteins were expressed and purified as described in a previous report [ ], except that BL21 Star (DE3) pLysS (Invitrogen, Carlsbad, CA, USA) was used. .. PCNA3-P450cam fusion protein, in which PCNA3 had a N106R mutation that improved the solubility of the fusion protein but did not affect PUPPET formation (unpublished data), was expressed and purified as described in the above report, except that T7 Express I q (New England BioLabs, Ipswich, MA, USA) was transformed with pET15+P3 N106R C. An approximately equimolar ratio of PCNA1-PdR and PCNA2-PdX and a slightly excess amount of PCNA3-P450cam were mixed and incubated on ice for 1 h. The mixture was subjected to size-exclusion chromatography on a Superdex 200 10/300 GL column (1.0 × 30 cm; GE Healthcare, Little Chalfont, Buckinghamshire, UK) with 50 mM potassium phosphate buffer, pH7.4, containing 150 mM potassium chloride and 2 mM d-camphor (Wako Pure Chemical Industries, Osaka, Japan).

    Size-exclusion Chromatography:

    Article Title: Fine Tuning of Spatial Arrangement of Enzymes in a PCNA-Mediated Multienzyme Complex Using a Rigid Poly-L-Proline Linker
    Article Snippet: Preparation of PUPPETs PCNA1-PdR and PCNA2-PdX fusion proteins were expressed and purified as described in a previous report [ ], except that BL21 Star (DE3) pLysS (Invitrogen, Carlsbad, CA, USA) was used. .. PCNA3-P450cam fusion protein, in which PCNA3 had a N106R mutation that improved the solubility of the fusion protein but did not affect PUPPET formation (unpublished data), was expressed and purified as described in the above report, except that T7 Express I q (New England BioLabs, Ipswich, MA, USA) was transformed with pET15+P3 N106R C. An approximately equimolar ratio of PCNA1-PdR and PCNA2-PdX and a slightly excess amount of PCNA3-P450cam were mixed and incubated on ice for 1 h. The mixture was subjected to size-exclusion chromatography on a Superdex 200 10/300 GL column (1.0 × 30 cm; GE Healthcare, Little Chalfont, Buckinghamshire, UK) with 50 mM potassium phosphate buffer, pH7.4, containing 150 mM potassium chloride and 2 mM d-camphor (Wako Pure Chemical Industries, Osaka, Japan).

    Blocking Assay:

    Article Title: A Full-Genomic Sequence-Verified Protein-Coding Gene Collection for Francisella tularensis
    Article Snippet: .. Briefly, BL21 star (DE3) pLysS (Invitrogen) transformants harboring the recombinant plasmids were grown at 37°C as 1 ml cultures in a 96-well block (Marsh Biomedical Products) to an OD600 of ∼0.7. ..

    Purification:

    Article Title: G-quadruplex recognition activities of E. Coli MutS
    Article Snippet: Paragraph title: Protein expression and purification ... Sequence verified pTX412 and mutant derivatives were transformed into BL21 Star (DE3) pLysS (Invitrogen) and purifications were performed from over-expressed cultures typically at the 0.5 L scale.

    Article Title: Fine Tuning of Spatial Arrangement of Enzymes in a PCNA-Mediated Multienzyme Complex Using a Rigid Poly-L-Proline Linker
    Article Snippet: .. Preparation of PUPPETs PCNA1-PdR and PCNA2-PdX fusion proteins were expressed and purified as described in a previous report [ ], except that BL21 Star (DE3) pLysS (Invitrogen, Carlsbad, CA, USA) was used. .. PCNA3-P450cam fusion protein, in which PCNA3 had a N106R mutation that improved the solubility of the fusion protein but did not affect PUPPET formation (unpublished data), was expressed and purified as described in the above report, except that T7 Express I q (New England BioLabs, Ipswich, MA, USA) was transformed with pET15+P3 N106R C. An approximately equimolar ratio of PCNA1-PdR and PCNA2-PdX and a slightly excess amount of PCNA3-P450cam were mixed and incubated on ice for 1 h. The mixture was subjected to size-exclusion chromatography on a Superdex 200 10/300 GL column (1.0 × 30 cm; GE Healthcare, Little Chalfont, Buckinghamshire, UK) with 50 mM potassium phosphate buffer, pH7.4, containing 150 mM potassium chloride and 2 mM d-camphor (Wako Pure Chemical Industries, Osaka, Japan).

    Sequencing:

    Article Title: G-quadruplex recognition activities of E. Coli MutS
    Article Snippet: .. Sequence verified pTX412 and mutant derivatives were transformed into BL21 Star (DE3) pLysS (Invitrogen) and purifications were performed from over-expressed cultures typically at the 0.5 L scale. ..

    Incubation:

    Article Title: TraG Encoded by the pIP501 Type IV Secretion System Is a Two-Domain Peptidoglycan-Degrading Enzyme Essential for Conjugative Transfer
    Article Snippet: .. The ligation mixtures were transformed into E. coli DH5α, Top10 (Life Technologies), XL10 Gold (Promega), and BL21 Star-pLysS (Life Technologies), respectively, and incubated at 37°C. ..

    Article Title: Fine Tuning of Spatial Arrangement of Enzymes in a PCNA-Mediated Multienzyme Complex Using a Rigid Poly-L-Proline Linker
    Article Snippet: Preparation of PUPPETs PCNA1-PdR and PCNA2-PdX fusion proteins were expressed and purified as described in a previous report [ ], except that BL21 Star (DE3) pLysS (Invitrogen, Carlsbad, CA, USA) was used. .. PCNA3-P450cam fusion protein, in which PCNA3 had a N106R mutation that improved the solubility of the fusion protein but did not affect PUPPET formation (unpublished data), was expressed and purified as described in the above report, except that T7 Express I q (New England BioLabs, Ipswich, MA, USA) was transformed with pET15+P3 N106R C. An approximately equimolar ratio of PCNA1-PdR and PCNA2-PdX and a slightly excess amount of PCNA3-P450cam were mixed and incubated on ice for 1 h. The mixture was subjected to size-exclusion chromatography on a Superdex 200 10/300 GL column (1.0 × 30 cm; GE Healthcare, Little Chalfont, Buckinghamshire, UK) with 50 mM potassium phosphate buffer, pH7.4, containing 150 mM potassium chloride and 2 mM d-camphor (Wako Pure Chemical Industries, Osaka, Japan).

    Amplification:

    Article Title: TraG Encoded by the pIP501 Type IV Secretion System Is a Two-Domain Peptidoglycan-Degrading Enzyme Essential for Conjugative Transfer
    Article Snippet: To complement the markerless traG deletion in trans , the expression vector pEU327 ( ) was selected. traG was amplified from pIP501 with TraG_SalI-fw and TraG_SalI-rev primers. .. The ligation mixtures were transformed into E. coli DH5α, Top10 (Life Technologies), XL10 Gold (Promega), and BL21 Star-pLysS (Life Technologies), respectively, and incubated at 37°C.

    Solubility:

    Article Title: Fine Tuning of Spatial Arrangement of Enzymes in a PCNA-Mediated Multienzyme Complex Using a Rigid Poly-L-Proline Linker
    Article Snippet: Preparation of PUPPETs PCNA1-PdR and PCNA2-PdX fusion proteins were expressed and purified as described in a previous report [ ], except that BL21 Star (DE3) pLysS (Invitrogen, Carlsbad, CA, USA) was used. .. PCNA3-P450cam fusion protein, in which PCNA3 had a N106R mutation that improved the solubility of the fusion protein but did not affect PUPPET formation (unpublished data), was expressed and purified as described in the above report, except that T7 Express I q (New England BioLabs, Ipswich, MA, USA) was transformed with pET15+P3 N106R C. An approximately equimolar ratio of PCNA1-PdR and PCNA2-PdX and a slightly excess amount of PCNA3-P450cam were mixed and incubated on ice for 1 h. The mixture was subjected to size-exclusion chromatography on a Superdex 200 10/300 GL column (1.0 × 30 cm; GE Healthcare, Little Chalfont, Buckinghamshire, UK) with 50 mM potassium phosphate buffer, pH7.4, containing 150 mM potassium chloride and 2 mM d-camphor (Wako Pure Chemical Industries, Osaka, Japan).

    Expressing:

    Article Title: G-quadruplex recognition activities of E. Coli MutS
    Article Snippet: Paragraph title: Protein expression and purification ... Sequence verified pTX412 and mutant derivatives were transformed into BL21 Star (DE3) pLysS (Invitrogen) and purifications were performed from over-expressed cultures typically at the 0.5 L scale.

    Article Title: A Full-Genomic Sequence-Verified Protein-Coding Gene Collection for Francisella tularensis
    Article Snippet: Paragraph title: Protein expression ... Briefly, BL21 star (DE3) pLysS (Invitrogen) transformants harboring the recombinant plasmids were grown at 37°C as 1 ml cultures in a 96-well block (Marsh Biomedical Products) to an OD600 of ∼0.7.

    Article Title: TraG Encoded by the pIP501 Type IV Secretion System Is a Two-Domain Peptidoglycan-Degrading Enzyme Essential for Conjugative Transfer
    Article Snippet: To complement the markerless traG deletion in trans , the expression vector pEU327 ( ) was selected. traG was amplified from pIP501 with TraG_SalI-fw and TraG_SalI-rev primers. .. The ligation mixtures were transformed into E. coli DH5α, Top10 (Life Technologies), XL10 Gold (Promega), and BL21 Star-pLysS (Life Technologies), respectively, and incubated at 37°C.

    Bradford Assay:

    Article Title: G-quadruplex recognition activities of E. Coli MutS
    Article Snippet: Sequence verified pTX412 and mutant derivatives were transformed into BL21 Star (DE3) pLysS (Invitrogen) and purifications were performed from over-expressed cultures typically at the 0.5 L scale. .. Purified MutS and MutS F36A was judged to be > 95% pure, as monitored by 6% SDS-PAGE, and the protein quantified by Bradford assay (BioRad, Hercules, CA) using BSA as a standard.

    Polymerase Chain Reaction:

    Article Title: TraG Encoded by the pIP501 Type IV Secretion System Is a Two-Domain Peptidoglycan-Degrading Enzyme Essential for Conjugative Transfer
    Article Snippet: The 1,109- and 1,132-bp PCR products were cut with SalI and inserted into pEU327/SalI. .. The ligation mixtures were transformed into E. coli DH5α, Top10 (Life Technologies), XL10 Gold (Promega), and BL21 Star-pLysS (Life Technologies), respectively, and incubated at 37°C.

    Transformation Assay:

    Article Title: G-quadruplex recognition activities of E. Coli MutS
    Article Snippet: .. Sequence verified pTX412 and mutant derivatives were transformed into BL21 Star (DE3) pLysS (Invitrogen) and purifications were performed from over-expressed cultures typically at the 0.5 L scale. ..

    Article Title: TraG Encoded by the pIP501 Type IV Secretion System Is a Two-Domain Peptidoglycan-Degrading Enzyme Essential for Conjugative Transfer
    Article Snippet: .. The ligation mixtures were transformed into E. coli DH5α, Top10 (Life Technologies), XL10 Gold (Promega), and BL21 Star-pLysS (Life Technologies), respectively, and incubated at 37°C. ..

    Article Title: Fine Tuning of Spatial Arrangement of Enzymes in a PCNA-Mediated Multienzyme Complex Using a Rigid Poly-L-Proline Linker
    Article Snippet: Preparation of PUPPETs PCNA1-PdR and PCNA2-PdX fusion proteins were expressed and purified as described in a previous report [ ], except that BL21 Star (DE3) pLysS (Invitrogen, Carlsbad, CA, USA) was used. .. PCNA3-P450cam fusion protein, in which PCNA3 had a N106R mutation that improved the solubility of the fusion protein but did not affect PUPPET formation (unpublished data), was expressed and purified as described in the above report, except that T7 Express I q (New England BioLabs, Ipswich, MA, USA) was transformed with pET15+P3 N106R C. An approximately equimolar ratio of PCNA1-PdR and PCNA2-PdX and a slightly excess amount of PCNA3-P450cam were mixed and incubated on ice for 1 h. The mixture was subjected to size-exclusion chromatography on a Superdex 200 10/300 GL column (1.0 × 30 cm; GE Healthcare, Little Chalfont, Buckinghamshire, UK) with 50 mM potassium phosphate buffer, pH7.4, containing 150 mM potassium chloride and 2 mM d-camphor (Wako Pure Chemical Industries, Osaka, Japan).

    Recombinant:

    Article Title: A Full-Genomic Sequence-Verified Protein-Coding Gene Collection for Francisella tularensis
    Article Snippet: .. Briefly, BL21 star (DE3) pLysS (Invitrogen) transformants harboring the recombinant plasmids were grown at 37°C as 1 ml cultures in a 96-well block (Marsh Biomedical Products) to an OD600 of ∼0.7. ..

    Over Expression:

    Article Title: G-quadruplex recognition activities of E. Coli MutS
    Article Snippet: Protein expression and purification Over expression and purification of MutS proteins followed previously published protocols using an original MutS expression clone pTX412 created by Malcom Winkler [ ] and provided to us by Dr. Peggy Hsieh (NIDDK, Bethesda MD). .. Sequence verified pTX412 and mutant derivatives were transformed into BL21 Star (DE3) pLysS (Invitrogen) and purifications were performed from over-expressed cultures typically at the 0.5 L scale.

    SDS Page:

    Article Title: G-quadruplex recognition activities of E. Coli MutS
    Article Snippet: Sequence verified pTX412 and mutant derivatives were transformed into BL21 Star (DE3) pLysS (Invitrogen) and purifications were performed from over-expressed cultures typically at the 0.5 L scale. .. Purified MutS and MutS F36A was judged to be > 95% pure, as monitored by 6% SDS-PAGE, and the protein quantified by Bradford assay (BioRad, Hercules, CA) using BSA as a standard.

    Plasmid Preparation:

    Article Title: TraG Encoded by the pIP501 Type IV Secretion System Is a Two-Domain Peptidoglycan-Degrading Enzyme Essential for Conjugative Transfer
    Article Snippet: To complement the markerless traG deletion in trans , the expression vector pEU327 ( ) was selected. traG was amplified from pIP501 with TraG_SalI-fw and TraG_SalI-rev primers. .. The ligation mixtures were transformed into E. coli DH5α, Top10 (Life Technologies), XL10 Gold (Promega), and BL21 Star-pLysS (Life Technologies), respectively, and incubated at 37°C.

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    Thermo Fisher bl21 star de3 plyss one shot chemically competent e coli
    Bl21 Star De3 Plyss One Shot Chemically Competent E Coli, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bl21 star de3 plyss one shot chemically competent e coli/product/Thermo Fisher
    Average 90 stars, based on 4 article reviews
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    Average 90 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
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