bl21 star de3 competent cells  (Thermo Fisher)


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    Name:
    MultiShot StripWell BL21 Star DE3 Competent Cells
    Description:
    MultiShot StripWell BL21 Star DE3 chemically competent E coli cells are cloning competent cells that are packaged in a rack containing 12 strips of 8 wells to increase productivity for medium throughput bacterial transformations MultiShot StripWell BL21 Star DE3 competent E coli cells are designed for applications that require high level expression of non toxic recombinant proteins from low copy number T7 promoter based expression systems e g Champion pET vectors MultiShot StripWell BL21 Star DE3 Competent Cells are provided at a transformation efficiency of 1 x 107 cfu µg plasmid DNA Enhanced expression of nontoxic recombinant proteins MultiShot StripWell BL21 Star DE3 Competent Cells contain the DE3 lysogen that carries the gene for T7 RNA polymerase under control of the lacUV5 promoter IPTG is required to induce expression This strain also offers enhanced mRNA stability due to a mutation in the RNaseE gene rne131 that reduces levels of endogenous RNases and mRNA degradation thereby increasing the stability of mRNA transcripts and increasing protein yield Protein expression is further enhanced by the absence of the lon and outer membrane OmpT proteases which reduces degradation of heterologous proteins Key features of the BL21 Star DE3 Competent Cells include • rne131 for promotion of high mRNA stability and protein yield • OmpT and lack of lon for minimal degradation of heterologous proteins by proteases • Optimized for use with low copy number T7 promoter based plasmids It should be noted that BL21 Star strains have higher basal expression of heterologous genes than BL21 strains due to the increased stability of mRNA Therefore these strains may not be useful for expression of toxic genes MultiShot StripWell format flexible for no waste Each StripWell of eight connected tubes is designed with the same single tube single use features as our One Shot format tubes This format allows for all steps of the transformation protocol up to plating to take place in the same tube thereby saving time and to preventing contamination After addition of DNA MultiShot StripWell chemically competent cells can be transformed by heat shock at 42°C using a water bath all in the same tube The StripWell tubes can be cut to permit transformations in a single tube eliminating wasted aliquots of cells and avoiding freeze thaws that can result in reduced transformation efficiency Key features of the MultiShot StripWell format include • Increase productivity for medium throughput experimentation • Maximal flexibility transform as few as 1 tube and as many as 96 from the StripWell rack • Familiar convenience no aliquotting add DNA directly to cells heat shock and recover in the same tube • Peace of mind single use transformation minimizes contamination Genotype F ompT hsdSB rB mB gal dcm rne131 DE3 Find the strain and format that you need We offer many other E coli strains and formats of chemically competent cells and electrocompetent cells to help meet your specific transformation needs If you require other high throughput transformation options choose from our collection of MultiShot formatted comp cells or email us for custom formatting options
    Catalog Number:
    c609601
    Price:
    None
    Applications:
    Bacterial Expression|Cloning|Protein Biology|Protein Expression|Transformation
    Category:
    Competent Cells Strains
    Buy from Supplier


    Structured Review

    Thermo Fisher bl21 star de3 competent cells
    MultiShot StripWell BL21 Star DE3 chemically competent E coli cells are cloning competent cells that are packaged in a rack containing 12 strips of 8 wells to increase productivity for medium throughput bacterial transformations MultiShot StripWell BL21 Star DE3 competent E coli cells are designed for applications that require high level expression of non toxic recombinant proteins from low copy number T7 promoter based expression systems e g Champion pET vectors MultiShot StripWell BL21 Star DE3 Competent Cells are provided at a transformation efficiency of 1 x 107 cfu µg plasmid DNA Enhanced expression of nontoxic recombinant proteins MultiShot StripWell BL21 Star DE3 Competent Cells contain the DE3 lysogen that carries the gene for T7 RNA polymerase under control of the lacUV5 promoter IPTG is required to induce expression This strain also offers enhanced mRNA stability due to a mutation in the RNaseE gene rne131 that reduces levels of endogenous RNases and mRNA degradation thereby increasing the stability of mRNA transcripts and increasing protein yield Protein expression is further enhanced by the absence of the lon and outer membrane OmpT proteases which reduces degradation of heterologous proteins Key features of the BL21 Star DE3 Competent Cells include • rne131 for promotion of high mRNA stability and protein yield • OmpT and lack of lon for minimal degradation of heterologous proteins by proteases • Optimized for use with low copy number T7 promoter based plasmids It should be noted that BL21 Star strains have higher basal expression of heterologous genes than BL21 strains due to the increased stability of mRNA Therefore these strains may not be useful for expression of toxic genes MultiShot StripWell format flexible for no waste Each StripWell of eight connected tubes is designed with the same single tube single use features as our One Shot format tubes This format allows for all steps of the transformation protocol up to plating to take place in the same tube thereby saving time and to preventing contamination After addition of DNA MultiShot StripWell chemically competent cells can be transformed by heat shock at 42°C using a water bath all in the same tube The StripWell tubes can be cut to permit transformations in a single tube eliminating wasted aliquots of cells and avoiding freeze thaws that can result in reduced transformation efficiency Key features of the MultiShot StripWell format include • Increase productivity for medium throughput experimentation • Maximal flexibility transform as few as 1 tube and as many as 96 from the StripWell rack • Familiar convenience no aliquotting add DNA directly to cells heat shock and recover in the same tube • Peace of mind single use transformation minimizes contamination Genotype F ompT hsdSB rB mB gal dcm rne131 DE3 Find the strain and format that you need We offer many other E coli strains and formats of chemically competent cells and electrocompetent cells to help meet your specific transformation needs If you require other high throughput transformation options choose from our collection of MultiShot formatted comp cells or email us for custom formatting options
    https://www.bioz.com/result/bl21 star de3 competent cells/product/Thermo Fisher
    Average 99 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    bl21 star de3 competent cells - by Bioz Stars, 2020-09
    99/100 stars

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    Related Articles

    Recombinant:

    Article Title: The DNA mimic protein BCAS0292 is involved in the regulation of virulence of Burkholderia cenocepacia
    Article Snippet: .. Following the confirmation of recombinant BCAS0292 (rBCAS0292) expression in a pilot study, 1 L cultures of BL21 cells transformed with the expression plasmid, were grown in LB-ampicillin (100 μg/ ml) and induced with IPTG at a final concentration of 1 mM overnight at 25 °C. .. Recombinant BCAS0292 purification Purification of rBCAS0292 was carried out as previously described ( ).

    Construct:

    Article Title: A dynamic regulatory interface on SARS-CoV-2 RNA polymerase
    Article Snippet: .. All constructs were transformed into Escherichia coli BL21(DE3) competent cells. ..

    Article Title: Versatile, Multivalent Nanobody Cocktails Efficiently Neutralize SARS-CoV-2
    Article Snippet: .. Expression and purification of proteinsNb DNA constructs were transformed into BL21(DE3) cells and plated on Agar with 50 μg/ml ampicillin at 37 °C overnight. .. Cells were cultured in an LB broth to reach an O.D. of ~ 0.5-0.6 before IPTG (0.5 mM) induct ion at 16°C overnight.

    Purification:

    Article Title: Versatile, Multivalent Nanobody Cocktails Efficiently Neutralize SARS-CoV-2
    Article Snippet: .. Expression and purification of proteinsNb DNA constructs were transformed into BL21(DE3) cells and plated on Agar with 50 μg/ml ampicillin at 37 °C overnight. .. Cells were cultured in an LB broth to reach an O.D. of ~ 0.5-0.6 before IPTG (0.5 mM) induct ion at 16°C overnight.

    Article Title: Golgi-resident TRIO regulates membrane trafficking during neurite outgrowth
    Article Snippet: .. Purification of the His6 -RABIN8 and direct binding assay His6 -RABIN8 were expressed in BL21(DE3) cells that had been induced with 1 m m IPTG at 37 °C for 4 h. Bacteria pellets were then sonicated in PBS with 1 mg/ml lysozyme. .. Proteins were then pelleted by centrifugation at 9000 rpm for 10 min and dissolved in PBS with 8 m urea.

    Concentration Assay:

    Article Title: The DNA mimic protein BCAS0292 is involved in the regulation of virulence of Burkholderia cenocepacia
    Article Snippet: .. Following the confirmation of recombinant BCAS0292 (rBCAS0292) expression in a pilot study, 1 L cultures of BL21 cells transformed with the expression plasmid, were grown in LB-ampicillin (100 μg/ ml) and induced with IPTG at a final concentration of 1 mM overnight at 25 °C. .. Recombinant BCAS0292 purification Purification of rBCAS0292 was carried out as previously described ( ).

    other:

    Article Title: Mapping RNAPII CTD Phosphorylation Reveals That the Identity and Modification of Seventh Heptad Residues Direct Tyr1 Phosphorylation
    Article Snippet: BL21 (DE3) cells coexpressing the human ABL1 kinase domain (residues 229−511) and YopH phosphatase from Yersinia were a kind gift from the Kuriyan lab.

    Expressing:

    Article Title: CRISPR/Cas9-based genome editing in the silverleaf whitefly (Bemisia tabaci)
    Article Snippet: .. Plasmids were transformed into BL21(DE3) cells for BtKV-Cas9/mCh expression following standard protein expression protocols. ..

    Article Title: The DNA mimic protein BCAS0292 is involved in the regulation of virulence of Burkholderia cenocepacia
    Article Snippet: .. Following the confirmation of recombinant BCAS0292 (rBCAS0292) expression in a pilot study, 1 L cultures of BL21 cells transformed with the expression plasmid, were grown in LB-ampicillin (100 μg/ ml) and induced with IPTG at a final concentration of 1 mM overnight at 25 °C. .. Recombinant BCAS0292 purification Purification of rBCAS0292 was carried out as previously described ( ).

    Article Title: Versatile, Multivalent Nanobody Cocktails Efficiently Neutralize SARS-CoV-2
    Article Snippet: .. Expression and purification of proteinsNb DNA constructs were transformed into BL21(DE3) cells and plated on Agar with 50 μg/ml ampicillin at 37 °C overnight. .. Cells were cultured in an LB broth to reach an O.D. of ~ 0.5-0.6 before IPTG (0.5 mM) induct ion at 16°C overnight.

    Sequencing:

    Article Title: Improving prognosis of surrogate assay for breast cancer patients by absolute quantitation of Ki67 protein levels using Quantitative Dot Blot (QDB) method
    Article Snippet: .. The plasmid was verified by sequencing,95 and expressed in BL21 (DE3) competent cells. .. The cells were induced with IPTG, and 96 total bacterial lysate was extracted in 10ml binding buffer (20 mM sodium phosphate, 97 500 mM NaCI, 20 mM imidazole, PH 7.4) before it was loaded onto a high affinity Ni2+ 98 column pre-equilibrated with 10ml binding buffer.

    Sonication:

    Article Title: Golgi-resident TRIO regulates membrane trafficking during neurite outgrowth
    Article Snippet: .. Purification of the His6 -RABIN8 and direct binding assay His6 -RABIN8 were expressed in BL21(DE3) cells that had been induced with 1 m m IPTG at 37 °C for 4 h. Bacteria pellets were then sonicated in PBS with 1 mg/ml lysozyme. .. Proteins were then pelleted by centrifugation at 9000 rpm for 10 min and dissolved in PBS with 8 m urea.

    Transformation Assay:

    Article Title: A dynamic regulatory interface on SARS-CoV-2 RNA polymerase
    Article Snippet: .. All constructs were transformed into Escherichia coli BL21(DE3) competent cells. ..

    Article Title: CRISPR/Cas9-based genome editing in the silverleaf whitefly (Bemisia tabaci)
    Article Snippet: .. Plasmids were transformed into BL21(DE3) cells for BtKV-Cas9/mCh expression following standard protein expression protocols. ..

    Article Title: The DNA mimic protein BCAS0292 is involved in the regulation of virulence of Burkholderia cenocepacia
    Article Snippet: .. Following the confirmation of recombinant BCAS0292 (rBCAS0292) expression in a pilot study, 1 L cultures of BL21 cells transformed with the expression plasmid, were grown in LB-ampicillin (100 μg/ ml) and induced with IPTG at a final concentration of 1 mM overnight at 25 °C. .. Recombinant BCAS0292 purification Purification of rBCAS0292 was carried out as previously described ( ).

    Article Title: Versatile, Multivalent Nanobody Cocktails Efficiently Neutralize SARS-CoV-2
    Article Snippet: .. Expression and purification of proteinsNb DNA constructs were transformed into BL21(DE3) cells and plated on Agar with 50 μg/ml ampicillin at 37 °C overnight. .. Cells were cultured in an LB broth to reach an O.D. of ~ 0.5-0.6 before IPTG (0.5 mM) induct ion at 16°C overnight.

    Article Title: Glucosamine-6P and glucosamine-1P, respectively an activator and a substrate of rhodococcal ADP-glucose pyrophosphorylases, show a hint to ascertain (actino)bacterial glucosamine metabolism
    Article Snippet: .. Competent E. coli BL21 (DE3) cells were transformed with the [pET28c/RfaglgC ] construction. .. Protein expression was carried out using LB medium (10 g/l tryptone; 5 g/l yeast extract; 5 g/l NaCl) supplemented with 100 g/ml kanamycin.

    Binding Assay:

    Article Title: Golgi-resident TRIO regulates membrane trafficking during neurite outgrowth
    Article Snippet: .. Purification of the His6 -RABIN8 and direct binding assay His6 -RABIN8 were expressed in BL21(DE3) cells that had been induced with 1 m m IPTG at 37 °C for 4 h. Bacteria pellets were then sonicated in PBS with 1 mg/ml lysozyme. .. Proteins were then pelleted by centrifugation at 9000 rpm for 10 min and dissolved in PBS with 8 m urea.

    Plasmid Preparation:

    Article Title: Improving prognosis of surrogate assay for breast cancer patients by absolute quantitation of Ki67 protein levels using Quantitative Dot Blot (QDB) method
    Article Snippet: .. The plasmid was verified by sequencing,95 and expressed in BL21 (DE3) competent cells. .. The cells were induced with IPTG, and 96 total bacterial lysate was extracted in 10ml binding buffer (20 mM sodium phosphate, 97 500 mM NaCI, 20 mM imidazole, PH 7.4) before it was loaded onto a high affinity Ni2+ 98 column pre-equilibrated with 10ml binding buffer.

    Article Title: The DNA mimic protein BCAS0292 is involved in the regulation of virulence of Burkholderia cenocepacia
    Article Snippet: .. Following the confirmation of recombinant BCAS0292 (rBCAS0292) expression in a pilot study, 1 L cultures of BL21 cells transformed with the expression plasmid, were grown in LB-ampicillin (100 μg/ ml) and induced with IPTG at a final concentration of 1 mM overnight at 25 °C. .. Recombinant BCAS0292 purification Purification of rBCAS0292 was carried out as previously described ( ).

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  • 99
    Thermo Fisher competent e coli bl21 star de3 cells
    Competent E Coli Bl21 Star De3 Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bl21 De3 Star Competent E Coli Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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      Buy from Supplier

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