bl21 plyss  (Thermo Fisher)


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    Structured Review

    Thermo Fisher bl21 plyss
    Bl21 Plyss, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bl21 plyss/product/Thermo Fisher
    Average 90 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    bl21 plyss - by Bioz Stars, 2020-02
    90/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Biological Activity of Recombinant Accessory Cholerae Enterotoxin (Ace) on Rabbit Ileal Loops and Antibacterial Assay
    Article Snippet: .. E. coli DH5α and BL21 (PlysS) were used for cloning and expression experiments (Invitrogen and Novagen, USA). .. Plasmid pET-28a+ (Novagen) was the expression vector.

    Article Title: EndoS2 is a unique and conserved enzyme of serotype M49 group A Streptococcus that hydrolyses N-linked glycans on IgG and ?1-acid glycoprotein
    Article Snippet: .. GAS was propagated on blood agar, Escherichia coli strains Top10 (Invitrogen) and BL21 pLysS (Invitrogen) were propagated on lysogeny broth agar and used for cloning and recombinant expression. ..

    Article Title: Coupling of NAD+ Biosynthesis and Nicotinamide Ribosyl Transport: Characterization of NadR Ribonucleotide Kinase Mutants of Haemophilus influenzae
    Article Snippet: E. coli strains ER2566 (New England Biolabs, Schwalbach, Germany) and TOP10F′ (Invitrogen) were employed for cloning plasmid constructs. .. BL21/pLysS (Invitrogen) was used for protein expression.

    Centrifugation:

    Article Title: Screening for FtsZ Dimerization Inhibitors Using Fluorescence Cross-Correlation Spectroscopy and Surface Resonance Plasmon Analysis
    Article Snippet: The proteins of FtsZK175D _N-terminal-EGFP and FtsZ_C-terminal-mCherry were expressed in E . coli , BL21 pLysS (Invitrogen), and incubated at 15°C for 20 hours with 0.5 mM IPTG. .. The cell lysate was recovered by centrifugation at 4°C.

    Amplification:

    Article Title: Screening for FtsZ Dimerization Inhibitors Using Fluorescence Cross-Correlation Spectroscopy and Surface Resonance Plasmon Analysis
    Article Snippet: Purification of FtsZ protein The sequences coding N- and C-terminal S . aureus FtsZ were amplified by PCR, fused with EGFP and mCherry (red fluorescent protein), respectively, and inserted into pRSET vector (Invitrogen). .. The proteins of FtsZK175D _N-terminal-EGFP and FtsZ_C-terminal-mCherry were expressed in E . coli , BL21 pLysS (Invitrogen), and incubated at 15°C for 20 hours with 0.5 mM IPTG.

    Filtration:

    Article Title: Screening for FtsZ Dimerization Inhibitors Using Fluorescence Cross-Correlation Spectroscopy and Surface Resonance Plasmon Analysis
    Article Snippet: The proteins of FtsZK175D _N-terminal-EGFP and FtsZ_C-terminal-mCherry were expressed in E . coli , BL21 pLysS (Invitrogen), and incubated at 15°C for 20 hours with 0.5 mM IPTG. .. After filtration, the lysate was purified using AKTAprime plus (GE Healthcare) installed in HisTrap HP and HiTrap Q HP columns (GE Healthcare) for Ni affinity and anion-exchange chromatography, respectively.

    Binding Assay:

    Article Title: Cross-Phosphorylation and Interaction between Src/FAK and MAPKAP5/PRAK in Early Focal Adhesions Controls Cell Motility
    Article Snippet: Expression of GST, GST-347PRAK, GST-PRAK-2, GST-paxillin, GST-HSP27 in BL21-pLysS (Invitrogen) was induced with 1 mM IPTG for 2–4 h at 30°C. .. Bacteria were collected and lysed in GST binding buffer (50 mM Tris-Cl pH 7.4, 1 mM EDTA, 100 mM NaCl) by freeze/thaw followed by sonication.

    Chromatography:

    Article Title: Screening for FtsZ Dimerization Inhibitors Using Fluorescence Cross-Correlation Spectroscopy and Surface Resonance Plasmon Analysis
    Article Snippet: The proteins of FtsZK175D _N-terminal-EGFP and FtsZ_C-terminal-mCherry were expressed in E . coli , BL21 pLysS (Invitrogen), and incubated at 15°C for 20 hours with 0.5 mM IPTG. .. After filtration, the lysate was purified using AKTAprime plus (GE Healthcare) installed in HisTrap HP and HiTrap Q HP columns (GE Healthcare) for Ni affinity and anion-exchange chromatography, respectively.

    Mutagenesis:

    Article Title: Screening for FtsZ Dimerization Inhibitors Using Fluorescence Cross-Correlation Spectroscopy and Surface Resonance Plasmon Analysis
    Article Snippet: A mutation was constructed at K175D to prevent further bundling of FtsZ in the N-terminal fragment. .. The proteins of FtsZK175D _N-terminal-EGFP and FtsZ_C-terminal-mCherry were expressed in E . coli , BL21 pLysS (Invitrogen), and incubated at 15°C for 20 hours with 0.5 mM IPTG.

    Plasmid Preparation:

    Article Title: Screening for FtsZ Dimerization Inhibitors Using Fluorescence Cross-Correlation Spectroscopy and Surface Resonance Plasmon Analysis
    Article Snippet: Purification of FtsZ protein The sequences coding N- and C-terminal S . aureus FtsZ were amplified by PCR, fused with EGFP and mCherry (red fluorescent protein), respectively, and inserted into pRSET vector (Invitrogen). .. The proteins of FtsZK175D _N-terminal-EGFP and FtsZ_C-terminal-mCherry were expressed in E . coli , BL21 pLysS (Invitrogen), and incubated at 15°C for 20 hours with 0.5 mM IPTG.

    Article Title: Biological Activity of Recombinant Accessory Cholerae Enterotoxin (Ace) on Rabbit Ileal Loops and Antibacterial Assay
    Article Snippet: E. coli DH5α and BL21 (PlysS) were used for cloning and expression experiments (Invitrogen and Novagen, USA). .. Plasmid pET-28a+ (Novagen) was the expression vector.

    Article Title: Coupling of NAD+ Biosynthesis and Nicotinamide Ribosyl Transport: Characterization of NadR Ribonucleotide Kinase Mutants of Haemophilus influenzae
    Article Snippet: E. coli strains ER2566 (New England Biolabs, Schwalbach, Germany) and TOP10F′ (Invitrogen) were employed for cloning plasmid constructs. .. BL21/pLysS (Invitrogen) was used for protein expression.

    Construct:

    Article Title: Screening for FtsZ Dimerization Inhibitors Using Fluorescence Cross-Correlation Spectroscopy and Surface Resonance Plasmon Analysis
    Article Snippet: A mutation was constructed at K175D to prevent further bundling of FtsZ in the N-terminal fragment. .. The proteins of FtsZK175D _N-terminal-EGFP and FtsZ_C-terminal-mCherry were expressed in E . coli , BL21 pLysS (Invitrogen), and incubated at 15°C for 20 hours with 0.5 mM IPTG.

    Article Title: Coupling of NAD+ Biosynthesis and Nicotinamide Ribosyl Transport: Characterization of NadR Ribonucleotide Kinase Mutants of Haemophilus influenzae
    Article Snippet: E. coli strains ER2566 (New England Biolabs, Schwalbach, Germany) and TOP10F′ (Invitrogen) were employed for cloning plasmid constructs. .. BL21/pLysS (Invitrogen) was used for protein expression.

    Purification:

    Article Title: Screening for FtsZ Dimerization Inhibitors Using Fluorescence Cross-Correlation Spectroscopy and Surface Resonance Plasmon Analysis
    Article Snippet: Paragraph title: Purification of FtsZ protein ... The proteins of FtsZK175D _N-terminal-EGFP and FtsZ_C-terminal-mCherry were expressed in E . coli , BL21 pLysS (Invitrogen), and incubated at 15°C for 20 hours with 0.5 mM IPTG.

    Protein Purification:

    Article Title: Cross-Phosphorylation and Interaction between Src/FAK and MAPKAP5/PRAK in Early Focal Adhesions Controls Cell Motility
    Article Snippet: Paragraph title: Protein purification ... Expression of GST, GST-347PRAK, GST-PRAK-2, GST-paxillin, GST-HSP27 in BL21-pLysS (Invitrogen) was induced with 1 mM IPTG for 2–4 h at 30°C.

    Concentration Assay:

    Article Title: Coupling of NAD+ Biosynthesis and Nicotinamide Ribosyl Transport: Characterization of NadR Ribonucleotide Kinase Mutants of Haemophilus influenzae
    Article Snippet: Antibiotics were used as described by Barcak et al. , except that the concentration of chloramphenicol was 0.5 μg/ml. .. BL21/pLysS (Invitrogen) was used for protein expression.

    Incubation:

    Article Title: Screening for FtsZ Dimerization Inhibitors Using Fluorescence Cross-Correlation Spectroscopy and Surface Resonance Plasmon Analysis
    Article Snippet: .. The proteins of FtsZK175D _N-terminal-EGFP and FtsZ_C-terminal-mCherry were expressed in E . coli , BL21 pLysS (Invitrogen), and incubated at 15°C for 20 hours with 0.5 mM IPTG. .. After harvest, cells were lysed by sonication in a buffer consisting of 50 mM Tris-HCl (pH 8.0), 250 mM KCl and 20 mM imidazol supplemented with the following protease inhibitors: 50 μM bestatin (ACROS, Inc.), 800 μM AEBSF (Santa Cruz), 15 μM E-64, 20 μM pepstatinA, 7 μM phosphoramidon, and 10 μM leupeptin (Peptide Institute, INC) on ice.

    Article Title: Cross-Phosphorylation and Interaction between Src/FAK and MAPKAP5/PRAK in Early Focal Adhesions Controls Cell Motility
    Article Snippet: Expression of GST, GST-347PRAK, GST-PRAK-2, GST-paxillin, GST-HSP27 in BL21-pLysS (Invitrogen) was induced with 1 mM IPTG for 2–4 h at 30°C. .. Supernatants were incubated with GST-Bind resin (Novagen), and protein was eluted using GST Elution Buffer (50 mM Tris-Cl pH 8.0, 10 mM reduced glutathione).

    Selection:

    Article Title: EndoS2 is a unique and conserved enzyme of serotype M49 group A Streptococcus that hydrolyses N-linked glycans on IgG and ?1-acid glycoprotein
    Article Snippet: GAS was propagated on blood agar, Escherichia coli strains Top10 (Invitrogen) and BL21 pLysS (Invitrogen) were propagated on lysogeny broth agar and used for cloning and recombinant expression. .. For selection in E. coli Top10 cells, carbenicillin was used at 100 μg·ml−1 and, for E. coli BL21 pLysS, 100 μg·ml−1 carbenicillin and 34 μg·ml−1 chloramphenicol were used.

    Article Title: Coupling of NAD+ Biosynthesis and Nicotinamide Ribosyl Transport: Characterization of NadR Ribonucleotide Kinase Mutants of Haemophilus influenzae
    Article Snippet: Kanamycin (50 μg/ml) or chloramphenicol (30 μg/ml) was added for selection of transformants using kanamycin. .. BL21/pLysS (Invitrogen) was used for protein expression.

    Cell Culture:

    Article Title: Biological Activity of Recombinant Accessory Cholerae Enterotoxin (Ace) on Rabbit Ileal Loops and Antibacterial Assay
    Article Snippet: E. coli DH5α and BL21 (PlysS) were used for cloning and expression experiments (Invitrogen and Novagen, USA). .. Bacteria were cultured in LB broth or on agar (Merck, Germany) with or without 30 µg kanamycin/ml (Sigma, USA).

    Article Title: Coupling of NAD+ Biosynthesis and Nicotinamide Ribosyl Transport: Characterization of NadR Ribonucleotide Kinase Mutants of Haemophilus influenzae
    Article Snippet: E. coli K-12 strains (Table ) were cultured in Luria-Bertani (LB) broth (Merck, Darmstadt, Germany) or on LB agar plates. .. BL21/pLysS (Invitrogen) was used for protein expression.

    Expressing:

    Article Title: Biological Activity of Recombinant Accessory Cholerae Enterotoxin (Ace) on Rabbit Ileal Loops and Antibacterial Assay
    Article Snippet: .. E. coli DH5α and BL21 (PlysS) were used for cloning and expression experiments (Invitrogen and Novagen, USA). .. Plasmid pET-28a+ (Novagen) was the expression vector.

    Article Title: Cross-Phosphorylation and Interaction between Src/FAK and MAPKAP5/PRAK in Early Focal Adhesions Controls Cell Motility
    Article Snippet: .. Expression of GST, GST-347PRAK, GST-PRAK-2, GST-paxillin, GST-HSP27 in BL21-pLysS (Invitrogen) was induced with 1 mM IPTG for 2–4 h at 30°C. .. Bacteria were collected and lysed in GST binding buffer (50 mM Tris-Cl pH 7.4, 1 mM EDTA, 100 mM NaCl) by freeze/thaw followed by sonication.

    Article Title: EndoS2 is a unique and conserved enzyme of serotype M49 group A Streptococcus that hydrolyses N-linked glycans on IgG and ?1-acid glycoprotein
    Article Snippet: .. GAS was propagated on blood agar, Escherichia coli strains Top10 (Invitrogen) and BL21 pLysS (Invitrogen) were propagated on lysogeny broth agar and used for cloning and recombinant expression. ..

    Article Title: Coupling of NAD+ Biosynthesis and Nicotinamide Ribosyl Transport: Characterization of NadR Ribonucleotide Kinase Mutants of Haemophilus influenzae
    Article Snippet: .. BL21/pLysS (Invitrogen) was used for protein expression. .. Isolation and preparation of chromosomal DNA were done by using the method of Grimberg et al. ( ).

    Sonication:

    Article Title: Screening for FtsZ Dimerization Inhibitors Using Fluorescence Cross-Correlation Spectroscopy and Surface Resonance Plasmon Analysis
    Article Snippet: The proteins of FtsZK175D _N-terminal-EGFP and FtsZ_C-terminal-mCherry were expressed in E . coli , BL21 pLysS (Invitrogen), and incubated at 15°C for 20 hours with 0.5 mM IPTG. .. After harvest, cells were lysed by sonication in a buffer consisting of 50 mM Tris-HCl (pH 8.0), 250 mM KCl and 20 mM imidazol supplemented with the following protease inhibitors: 50 μM bestatin (ACROS, Inc.), 800 μM AEBSF (Santa Cruz), 15 μM E-64, 20 μM pepstatinA, 7 μM phosphoramidon, and 10 μM leupeptin (Peptide Institute, INC) on ice.

    Article Title: Cross-Phosphorylation and Interaction between Src/FAK and MAPKAP5/PRAK in Early Focal Adhesions Controls Cell Motility
    Article Snippet: Expression of GST, GST-347PRAK, GST-PRAK-2, GST-paxillin, GST-HSP27 in BL21-pLysS (Invitrogen) was induced with 1 mM IPTG for 2–4 h at 30°C. .. Bacteria were collected and lysed in GST binding buffer (50 mM Tris-Cl pH 7.4, 1 mM EDTA, 100 mM NaCl) by freeze/thaw followed by sonication.

    Polymerase Chain Reaction:

    Article Title: Screening for FtsZ Dimerization Inhibitors Using Fluorescence Cross-Correlation Spectroscopy and Surface Resonance Plasmon Analysis
    Article Snippet: Purification of FtsZ protein The sequences coding N- and C-terminal S . aureus FtsZ were amplified by PCR, fused with EGFP and mCherry (red fluorescent protein), respectively, and inserted into pRSET vector (Invitrogen). .. The proteins of FtsZK175D _N-terminal-EGFP and FtsZ_C-terminal-mCherry were expressed in E . coli , BL21 pLysS (Invitrogen), and incubated at 15°C for 20 hours with 0.5 mM IPTG.

    Recombinant:

    Article Title: EndoS2 is a unique and conserved enzyme of serotype M49 group A Streptococcus that hydrolyses N-linked glycans on IgG and ?1-acid glycoprotein
    Article Snippet: .. GAS was propagated on blood agar, Escherichia coli strains Top10 (Invitrogen) and BL21 pLysS (Invitrogen) were propagated on lysogeny broth agar and used for cloning and recombinant expression. ..

    Positron Emission Tomography:

    Article Title: Biological Activity of Recombinant Accessory Cholerae Enterotoxin (Ace) on Rabbit Ileal Loops and Antibacterial Assay
    Article Snippet: E. coli DH5α and BL21 (PlysS) were used for cloning and expression experiments (Invitrogen and Novagen, USA). .. Plasmid pET-28a+ (Novagen) was the expression vector.

    Molecular Weight:

    Article Title: Cross-Phosphorylation and Interaction between Src/FAK and MAPKAP5/PRAK in Early Focal Adhesions Controls Cell Motility
    Article Snippet: Expression of GST, GST-347PRAK, GST-PRAK-2, GST-paxillin, GST-HSP27 in BL21-pLysS (Invitrogen) was induced with 1 mM IPTG for 2–4 h at 30°C. .. Eluted protein was dialyzed 1:1000 into PBS at 4°C using Slide-a-Lyzer cartridges (Pierce) or de-salted and concentrated using Amicon ultra centrifugal filters with 10 kDa molecular weight cutoff (Millipore).

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    Thermo Fisher bl21 star de3 plyss one shot chemically competent e coli
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