bl21 plyss  (Millipore)


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    Millipore bl21 plyss
    Bl21 Plyss, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bl21 plyss/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bl21 plyss - by Bioz Stars, 2020-04
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    Clone Assay:

    Article Title: A simple vector system to improve performance and utilisation of recombinant antibodies
    Article Snippet: Paragraph title: Reagents, scFv clones & bacterial strains ... E. coli BL21 (DE3) and Origami 2™ (DE3) cells and the pET26b(+) plasmid were from Novagen (San Diego, CA).

    Article Title: Five Proteins of Laodelphax striatellus Are Potentially Involved in the Interactions between Rice Stripe Virus and Vector
    Article Snippet: Binding of expressed L. striatellus proteins to RSV particles in vitro ORFs of five virus-binding proteins were amplified using Pfu DNA polymerase and specific primer pairs , and cloned individually into a prokaryotic expression vector, pET-32a (Novagen). .. The recombinant plasmids, which were designated pET-RACK, pET-RPL5, pET-GAPDH3, pET-RPL7a and pET-RPL8, were sequenced to confirm their authenticity, and then transformed respectively into E. coli strain BL21 (DE3) pLys S (Novagen).

    Article Title: Genomic and molecular characterization of a novel quorum sensing molecule in Bacillus licheniformis
    Article Snippet: The expression strain [E. coli BL21 (DE3)] and E. coli TOP10 were used for cloning/transformations and were selected on LBA supplemented with ampicilin (100 µgml−1 ). .. E. coli BL21 ComX producer strain was cultivated in M9 minimal salts solution (sigma).

    Article Title: The gaf Fimbrial Gene Cluster of Escherichia coli Expresses a Full-Size and a Truncated Soluble Adhesin Protein
    Article Snippet: Plasmid pRR-5 contains the complete gaf gene cluster from E. coli strain IHE11165 on a 7-kb DNA fragment in pACYC184 , and pHUB110 contains a 6-bp in-frame deletion within the coding region of gafD , resulting in G fimbriae lacking GlcNAc-binding capacity ( ). pHUB113 ( ) contains the gafD reading frame cloned into pUC19. .. E. coli strain BL21 λDE3 ( ompT lon ) and expression vector pET-22b(+) were from Novagen Inc. (Madison, Wis.).

    Centrifugation:

    Article Title: A new hybrid bacteriocin, Ent35-MccV, displays antimicrobial activity against pathogenic Gram-positive and Gram-negative bacteria
    Article Snippet: 2.3 Gene expression and bacteriocins purification for activity determinations Ent35–MccV was expressed from pMA24 in E. coli BL21 [DE3] (pLysS) grown in LB (Sigma) at 37 °C. .. After 3 h, cells were collected by centrifugation in 10 mM HEPES, pH 7, PMSF 0.5 mM (buffer A), lysed using a French press and centrifuged at 47,000xg , 4 °C for 1 h. The supernatant was heated (10 min, 100 °C) and centrifuged (10 min, 10,000xg ).

    Article Title: Hypoallergenic Der p 1/Der p 2 combination vaccines for immunotherapy of house dust mite allergy
    Article Snippet: Expression plasmids containing the Der p 2/1 constructs were transformed into E coli strain BL21 (DE3; Novagen, Merck). .. Protein expression was performed in 250 mL of liquid culture by means of induction with 0.5 mmol/L isopropyl-β-D-1-thiogalactopyranoside at an OD600 of 0.8 for 4 hours at 37°C, and cells were harvested by means of centrifugation at 4000 g for 15 minutes at 4°C.

    Amplification:

    Article Title: Five Proteins of Laodelphax striatellus Are Potentially Involved in the Interactions between Rice Stripe Virus and Vector
    Article Snippet: Binding of expressed L. striatellus proteins to RSV particles in vitro ORFs of five virus-binding proteins were amplified using Pfu DNA polymerase and specific primer pairs , and cloned individually into a prokaryotic expression vector, pET-32a (Novagen). .. The recombinant plasmids, which were designated pET-RACK, pET-RPL5, pET-GAPDH3, pET-RPL7a and pET-RPL8, were sequenced to confirm their authenticity, and then transformed respectively into E. coli strain BL21 (DE3) pLys S (Novagen).

    Synthesized:

    Article Title: Fluorescence Resonance Energy Transfer Assay for High-Throughput Screening of ADAMTS1 Inhibitors
    Article Snippet: Materials E . coli BL21 (DE3), E. coli DH5a, pET32a(+) vector and his-bind purification kit were purchased from Novagen. .. The peptide, Abz-YPLPRNITEGEARGNVILTAK(Dnp)P-OH, was synthesized by GL Biochem Ltd. (Shanghai, China).

    Article Title: Preparation and Antitumor Activity of CS5931, A Novel Polypeptide from Sea Squirt Ciona Savignyi
    Article Snippet: .. Recombinant Expression of CS5931 from E. coli BL21 (DE3) The expression vector, pET28a/CS5931 synthesized by our lab previously [ ], was transfected into E. coli BL21 (DE3) cells (Novagen, Darmstadt, Germany). .. Cells were incubated in low-salt LB medium (containing 100 µg/mL Kanamycin) at 37 °C with shaking at 200 rpm.

    Construct:

    Article Title: Hypoallergenic Der p 1/Der p 2 combination vaccines for immunotherapy of house dust mite allergy
    Article Snippet: .. Expression plasmids containing the Der p 2/1 constructs were transformed into E coli strain BL21 (DE3; Novagen, Merck). .. Protein expression was performed in 250 mL of liquid culture by means of induction with 0.5 mmol/L isopropyl-β-D-1-thiogalactopyranoside at an OD600 of 0.8 for 4 hours at 37°C, and cells were harvested by means of centrifugation at 4000 g for 15 minutes at 4°C.

    Incubation:

    Article Title: Hypoallergenic Der p 1/Der p 2 combination vaccines for immunotherapy of house dust mite allergy
    Article Snippet: Expression plasmids containing the Der p 2/1 constructs were transformed into E coli strain BL21 (DE3; Novagen, Merck). .. Cells were lysed by using 3 freeze/thaw cycles (−70°C/+50°C), DNA was degraded by means of incubation with 1 μg of DNase I for 10 minutes at room temperature, and cell debris was removed by means of centrifugation (10,000 g for 30 minutes at 4°C).

    Article Title: Preparation and Antitumor Activity of CS5931, A Novel Polypeptide from Sea Squirt Ciona Savignyi
    Article Snippet: Recombinant Expression of CS5931 from E. coli BL21 (DE3) The expression vector, pET28a/CS5931 synthesized by our lab previously [ ], was transfected into E. coli BL21 (DE3) cells (Novagen, Darmstadt, Germany). .. Cells were incubated in low-salt LB medium (containing 100 µg/mL Kanamycin) at 37 °C with shaking at 200 rpm.

    Activity Assay:

    Article Title: A new hybrid bacteriocin, Ent35-MccV, displays antimicrobial activity against pathogenic Gram-positive and Gram-negative bacteria
    Article Snippet: .. 2.3 Gene expression and bacteriocins purification for activity determinations Ent35–MccV was expressed from pMA24 in E. coli BL21 [DE3] (pLysS) grown in LB (Sigma) at 37 °C. ..

    Expressing:

    Article Title: A new hybrid bacteriocin, Ent35-MccV, displays antimicrobial activity against pathogenic Gram-positive and Gram-negative bacteria
    Article Snippet: .. 2.3 Gene expression and bacteriocins purification for activity determinations Ent35–MccV was expressed from pMA24 in E. coli BL21 [DE3] (pLysS) grown in LB (Sigma) at 37 °C. ..

    Article Title: Hypoallergenic Der p 1/Der p 2 combination vaccines for immunotherapy of house dust mite allergy
    Article Snippet: .. Expression plasmids containing the Der p 2/1 constructs were transformed into E coli strain BL21 (DE3; Novagen, Merck). .. Protein expression was performed in 250 mL of liquid culture by means of induction with 0.5 mmol/L isopropyl-β-D-1-thiogalactopyranoside at an OD600 of 0.8 for 4 hours at 37°C, and cells were harvested by means of centrifugation at 4000 g for 15 minutes at 4°C.

    Article Title: Five Proteins of Laodelphax striatellus Are Potentially Involved in the Interactions between Rice Stripe Virus and Vector
    Article Snippet: Binding of expressed L. striatellus proteins to RSV particles in vitro ORFs of five virus-binding proteins were amplified using Pfu DNA polymerase and specific primer pairs , and cloned individually into a prokaryotic expression vector, pET-32a (Novagen). .. The recombinant plasmids, which were designated pET-RACK, pET-RPL5, pET-GAPDH3, pET-RPL7a and pET-RPL8, were sequenced to confirm their authenticity, and then transformed respectively into E. coli strain BL21 (DE3) pLys S (Novagen).

    Article Title: Chemical complexity of protein determines optimal E. coli expression host; A comparative study using Erythropoietin, Streptokinase and Tumor Necrosis Factor Receptor
    Article Snippet: The bacterial strains used to study expression were E. coli GJ1158, E. coli BL21 (DE3), E. coli BL21 (DE3) pLys S, E. coli BL21 Rosetta (DE3) pLys S. E. coli GJ1158 , derived from E. coli B strain BL21, is a salt inducible strain with a pro U promoter ( ). .. E. coli BL21 (DE3), E. coli BL21 (DE3) pLys S, E. coli BL21 Rosetta (DE3) pLys S (Novagen,) are IPTG inducible strains in which the T7 RNA Polymerase gene is under the control of a Lac UV5 promoter.

    Article Title: Genomic and molecular characterization of a novel quorum sensing molecule in Bacillus licheniformis
    Article Snippet: The expression strain [E. coli BL21 (DE3)] and E. coli TOP10 were used for cloning/transformations and were selected on LBA supplemented with ampicilin (100 µgml−1 ). .. E. coli BL21 ComX producer strain was cultivated in M9 minimal salts solution (sigma).

    Article Title: The gaf Fimbrial Gene Cluster of Escherichia coli Expresses a Full-Size and a Truncated Soluble Adhesin Protein
    Article Snippet: .. E. coli strain BL21 λDE3 ( ompT lon ) and expression vector pET-22b(+) were from Novagen Inc. (Madison, Wis.). .. The bacteria were cultivated at 37°C in Luria broth ( ) containing the appropriate antibiotics.

    Article Title: Preparation and Antitumor Activity of CS5931, A Novel Polypeptide from Sea Squirt Ciona Savignyi
    Article Snippet: .. Recombinant Expression of CS5931 from E. coli BL21 (DE3) The expression vector, pET28a/CS5931 synthesized by our lab previously [ ], was transfected into E. coli BL21 (DE3) cells (Novagen, Darmstadt, Germany). .. Cells were incubated in low-salt LB medium (containing 100 µg/mL Kanamycin) at 37 °C with shaking at 200 rpm.

    Transformation Assay:

    Article Title: Hypoallergenic Der p 1/Der p 2 combination vaccines for immunotherapy of house dust mite allergy
    Article Snippet: .. Expression plasmids containing the Der p 2/1 constructs were transformed into E coli strain BL21 (DE3; Novagen, Merck). .. Protein expression was performed in 250 mL of liquid culture by means of induction with 0.5 mmol/L isopropyl-β-D-1-thiogalactopyranoside at an OD600 of 0.8 for 4 hours at 37°C, and cells were harvested by means of centrifugation at 4000 g for 15 minutes at 4°C.

    Article Title: Five Proteins of Laodelphax striatellus Are Potentially Involved in the Interactions between Rice Stripe Virus and Vector
    Article Snippet: .. The recombinant plasmids, which were designated pET-RACK, pET-RPL5, pET-GAPDH3, pET-RPL7a and pET-RPL8, were sequenced to confirm their authenticity, and then transformed respectively into E. coli strain BL21 (DE3) pLys S (Novagen). ..

    Article Title: Chemical complexity of protein determines optimal E. coli expression host; A comparative study using Erythropoietin, Streptokinase and Tumor Necrosis Factor Receptor
    Article Snippet: 2.1 Bacterial strains and plasmids For initial transformation of the ligation mixtures, the maintenance strain E. coli DH5α was used. .. E. coli BL21 (DE3), E. coli BL21 (DE3) pLys S, E. coli BL21 Rosetta (DE3) pLys S (Novagen,) are IPTG inducible strains in which the T7 RNA Polymerase gene is under the control of a Lac UV5 promoter.

    Derivative Assay:

    Article Title: Chemical complexity of protein determines optimal E. coli expression host; A comparative study using Erythropoietin, Streptokinase and Tumor Necrosis Factor Receptor
    Article Snippet: The bacterial strains used to study expression were E. coli GJ1158, E. coli BL21 (DE3), E. coli BL21 (DE3) pLys S, E. coli BL21 Rosetta (DE3) pLys S. E. coli GJ1158 , derived from E. coli B strain BL21, is a salt inducible strain with a pro U promoter ( ). .. E. coli BL21 (DE3), E. coli BL21 (DE3) pLys S, E. coli BL21 Rosetta (DE3) pLys S (Novagen,) are IPTG inducible strains in which the T7 RNA Polymerase gene is under the control of a Lac UV5 promoter.

    Transfection:

    Article Title: Preparation and Antitumor Activity of CS5931, A Novel Polypeptide from Sea Squirt Ciona Savignyi
    Article Snippet: .. Recombinant Expression of CS5931 from E. coli BL21 (DE3) The expression vector, pET28a/CS5931 synthesized by our lab previously [ ], was transfected into E. coli BL21 (DE3) cells (Novagen, Darmstadt, Germany). .. Cells were incubated in low-salt LB medium (containing 100 µg/mL Kanamycin) at 37 °C with shaking at 200 rpm.

    Ligation:

    Article Title: Chemical complexity of protein determines optimal E. coli expression host; A comparative study using Erythropoietin, Streptokinase and Tumor Necrosis Factor Receptor
    Article Snippet: 2.1 Bacterial strains and plasmids For initial transformation of the ligation mixtures, the maintenance strain E. coli DH5α was used. .. E. coli BL21 (DE3), E. coli BL21 (DE3) pLys S, E. coli BL21 Rosetta (DE3) pLys S (Novagen,) are IPTG inducible strains in which the T7 RNA Polymerase gene is under the control of a Lac UV5 promoter.

    Cell Culture:

    Article Title: A novel HPV16 E7-affitoxin for targeted therapy of HPV16-induced human cervical cancer
    Article Snippet: SiHa (ATCC: HTB-35, HPV16 positive, contains about one to two copies of integrated HPV16 genome), CaSki (ATCC: CRL-1550, HPV16 positive, contains about 600 copies of integrated HPV16 genome), HeLa 229 (ATCC: CCL-2.1, HPV18 positive, used as HPV16 negative control cell line), and melanoma tumor A375 (ATCC: CRL-1619, used as HPV negative control cell line) were obtained from the American Type Culture Collection (ATCC, USA) and cultured as previously described . .. The pET21a(+) vector and E.coli BL21 (DE3) were purchased from Novagen and ATCC, respectively.

    Generated:

    Article Title: A simple vector system to improve performance and utilisation of recombinant antibodies
    Article Snippet: E. coli BL21 (DE3) and Origami 2™ (DE3) cells and the pET26b(+) plasmid were from Novagen (San Diego, CA). .. The other scFvs were generated in house to commercially available antigens purchased from R & D Systems (MN, USA), USBiologicals (MA, USA) and Progen (Heidelberg, Germany).

    Binding Assay:

    Article Title: Five Proteins of Laodelphax striatellus Are Potentially Involved in the Interactions between Rice Stripe Virus and Vector
    Article Snippet: Paragraph title: Binding of expressed L. striatellus proteins to RSV particles in vitro ... The recombinant plasmids, which were designated pET-RACK, pET-RPL5, pET-GAPDH3, pET-RPL7a and pET-RPL8, were sequenced to confirm their authenticity, and then transformed respectively into E. coli strain BL21 (DE3) pLys S (Novagen).

    Purification:

    Article Title: A new hybrid bacteriocin, Ent35-MccV, displays antimicrobial activity against pathogenic Gram-positive and Gram-negative bacteria
    Article Snippet: .. 2.3 Gene expression and bacteriocins purification for activity determinations Ent35–MccV was expressed from pMA24 in E. coli BL21 [DE3] (pLysS) grown in LB (Sigma) at 37 °C. ..

    Article Title: Hypoallergenic Der p 1/Der p 2 combination vaccines for immunotherapy of house dust mite allergy
    Article Snippet: Paragraph title: Expression and purification of Der p 2/1 mosaic proteins ... Expression plasmids containing the Der p 2/1 constructs were transformed into E coli strain BL21 (DE3; Novagen, Merck).

    Article Title: Fluorescence Resonance Energy Transfer Assay for High-Throughput Screening of ADAMTS1 Inhibitors
    Article Snippet: .. Materials E . coli BL21 (DE3), E. coli DH5a, pET32a(+) vector and his-bind purification kit were purchased from Novagen. .. Heparin sepharose CL-6B column was purchased from Amersham Biosciences.

    Mouse Assay:

    Article Title: A novel HPV16 E7-affitoxin for targeted therapy of HPV16-induced human cervical cancer
    Article Snippet: ICR mice, weighing 23-27 g, were purchased from the animal experimental center of Wenzhou Medical University, China. .. The pET21a(+) vector and E.coli BL21 (DE3) were purchased from Novagen and ATCC, respectively.

    Plasmid Preparation:

    Article Title: A simple vector system to improve performance and utilisation of recombinant antibodies
    Article Snippet: .. E. coli BL21 (DE3) and Origami 2™ (DE3) cells and the pET26b(+) plasmid were from Novagen (San Diego, CA). ..

    Article Title: Five Proteins of Laodelphax striatellus Are Potentially Involved in the Interactions between Rice Stripe Virus and Vector
    Article Snippet: Binding of expressed L. striatellus proteins to RSV particles in vitro ORFs of five virus-binding proteins were amplified using Pfu DNA polymerase and specific primer pairs , and cloned individually into a prokaryotic expression vector, pET-32a (Novagen). .. The recombinant plasmids, which were designated pET-RACK, pET-RPL5, pET-GAPDH3, pET-RPL7a and pET-RPL8, were sequenced to confirm their authenticity, and then transformed respectively into E. coli strain BL21 (DE3) pLys S (Novagen).

    Article Title: Chemical complexity of protein determines optimal E. coli expression host; A comparative study using Erythropoietin, Streptokinase and Tumor Necrosis Factor Receptor
    Article Snippet: E. coli BL21 (DE3), E. coli BL21 (DE3) pLys S, E. coli BL21 Rosetta (DE3) pLys S (Novagen,) are IPTG inducible strains in which the T7 RNA Polymerase gene is under the control of a Lac UV5 promoter. .. The expression vector used in this study is pRSET A obtained from Invitrogen Life Technologies, USA. pRSET is a high copy number plasmid with a pUC origin of replication.

    Article Title: A Single Codon Optimization Enhances Recombinant Human TNF-α Vaccine Expression in Escherichia coli
    Article Snippet: .. The vector pET-22b and E. coli strain BL21 (DE3) were purchased from Novagen (San Diego, CA). .. Isopropyl β -D-1-thiogalactopyranoside (IPTG) was from Sigma-Aldrich (St. Louis, MO).

    Article Title: The gaf Fimbrial Gene Cluster of Escherichia coli Expresses a Full-Size and a Truncated Soluble Adhesin Protein
    Article Snippet: .. E. coli strain BL21 λDE3 ( ompT lon ) and expression vector pET-22b(+) were from Novagen Inc. (Madison, Wis.). .. The bacteria were cultivated at 37°C in Luria broth ( ) containing the appropriate antibiotics.

    Article Title: A novel HPV16 E7-affitoxin for targeted therapy of HPV16-induced human cervical cancer
    Article Snippet: .. The pET21a(+) vector and E.coli BL21 (DE3) were purchased from Novagen and ATCC, respectively. .. Reagents The reagents used, including Cell Counting Kit-8 (CCK-8) (Dojindo, Japan), RPMI-1640 (Gibco, USA), fetal bovine serum (FBS) (Gibco, USA), penicillin (Gibco, USA), trypsin-EDTA (Gibco, USA), streptomycin (Sigma Aldrich, Saint Louis, USA), Isopropyl-D-thiogalactopyranoside (IPTG) (Sigma Aldrich, Saint Louis, USA), Ni-NTA agarose (Qiagen Inc., Valencia, CA), and DyLight-755 (Thermo Fisher Scientific, USA), were purchased from commercial sources.

    Article Title: Fluorescence Resonance Energy Transfer Assay for High-Throughput Screening of ADAMTS1 Inhibitors
    Article Snippet: .. Materials E . coli BL21 (DE3), E. coli DH5a, pET32a(+) vector and his-bind purification kit were purchased from Novagen. .. Heparin sepharose CL-6B column was purchased from Amersham Biosciences.

    Article Title: Preparation and Antitumor Activity of CS5931, A Novel Polypeptide from Sea Squirt Ciona Savignyi
    Article Snippet: .. Recombinant Expression of CS5931 from E. coli BL21 (DE3) The expression vector, pET28a/CS5931 synthesized by our lab previously [ ], was transfected into E. coli BL21 (DE3) cells (Novagen, Darmstadt, Germany). .. Cells were incubated in low-salt LB medium (containing 100 µg/mL Kanamycin) at 37 °C with shaking at 200 rpm.

    Article Title: Identification and molecular characterization of a metagenome-derived L-lysine decarboxylase gene from subtropical soil microorganisms
    Article Snippet: .. Plasmid pET-30a(+) (Novagen) and bacterial strain E . coli BL21 (DE3) pLysS (Novagen) were used as the expression vector and host, respectively. ..

    Negative Control:

    Article Title: A novel HPV16 E7-affitoxin for targeted therapy of HPV16-induced human cervical cancer
    Article Snippet: SiHa (ATCC: HTB-35, HPV16 positive, contains about one to two copies of integrated HPV16 genome), CaSki (ATCC: CRL-1550, HPV16 positive, contains about 600 copies of integrated HPV16 genome), HeLa 229 (ATCC: CCL-2.1, HPV18 positive, used as HPV16 negative control cell line), and melanoma tumor A375 (ATCC: CRL-1619, used as HPV negative control cell line) were obtained from the American Type Culture Collection (ATCC, USA) and cultured as previously described . .. The pET21a(+) vector and E.coli BL21 (DE3) were purchased from Novagen and ATCC, respectively.

    Positron Emission Tomography:

    Article Title: Five Proteins of Laodelphax striatellus Are Potentially Involved in the Interactions between Rice Stripe Virus and Vector
    Article Snippet: .. The recombinant plasmids, which were designated pET-RACK, pET-RPL5, pET-GAPDH3, pET-RPL7a and pET-RPL8, were sequenced to confirm their authenticity, and then transformed respectively into E. coli strain BL21 (DE3) pLys S (Novagen). ..

    Article Title: A Single Codon Optimization Enhances Recombinant Human TNF-α Vaccine Expression in Escherichia coli
    Article Snippet: .. The vector pET-22b and E. coli strain BL21 (DE3) were purchased from Novagen (San Diego, CA). .. Isopropyl β -D-1-thiogalactopyranoside (IPTG) was from Sigma-Aldrich (St. Louis, MO).

    Article Title: The gaf Fimbrial Gene Cluster of Escherichia coli Expresses a Full-Size and a Truncated Soluble Adhesin Protein
    Article Snippet: .. E. coli strain BL21 λDE3 ( ompT lon ) and expression vector pET-22b(+) were from Novagen Inc. (Madison, Wis.). .. The bacteria were cultivated at 37°C in Luria broth ( ) containing the appropriate antibiotics.

    Article Title: Identification and molecular characterization of a metagenome-derived L-lysine decarboxylase gene from subtropical soil microorganisms
    Article Snippet: .. Plasmid pET-30a(+) (Novagen) and bacterial strain E . coli BL21 (DE3) pLysS (Novagen) were used as the expression vector and host, respectively. ..

    In Vitro:

    Article Title: Five Proteins of Laodelphax striatellus Are Potentially Involved in the Interactions between Rice Stripe Virus and Vector
    Article Snippet: Paragraph title: Binding of expressed L. striatellus proteins to RSV particles in vitro ... The recombinant plasmids, which were designated pET-RACK, pET-RPL5, pET-GAPDH3, pET-RPL7a and pET-RPL8, were sequenced to confirm their authenticity, and then transformed respectively into E. coli strain BL21 (DE3) pLys S (Novagen).

    Concentration Assay:

    Article Title: Preparation and Antitumor Activity of CS5931, A Novel Polypeptide from Sea Squirt Ciona Savignyi
    Article Snippet: Recombinant Expression of CS5931 from E. coli BL21 (DE3) The expression vector, pET28a/CS5931 synthesized by our lab previously [ ], was transfected into E. coli BL21 (DE3) cells (Novagen, Darmstadt, Germany). .. After the value of OD600 of the culture medium reached around 0.5–0.7, IPTG was added at a final concentration of 0.75 mM and the cells were incubated overnight at 16 °C.

    Recombinant:

    Article Title: Five Proteins of Laodelphax striatellus Are Potentially Involved in the Interactions between Rice Stripe Virus and Vector
    Article Snippet: .. The recombinant plasmids, which were designated pET-RACK, pET-RPL5, pET-GAPDH3, pET-RPL7a and pET-RPL8, were sequenced to confirm their authenticity, and then transformed respectively into E. coli strain BL21 (DE3) pLys S (Novagen). ..

    Article Title: Fluorescence Resonance Energy Transfer Assay for High-Throughput Screening of ADAMTS1 Inhibitors
    Article Snippet: Materials E . coli BL21 (DE3), E. coli DH5a, pET32a(+) vector and his-bind purification kit were purchased from Novagen. .. Recombinant human ADAMTS1 was purchased from R & D Systems.

    Article Title: Preparation and Antitumor Activity of CS5931, A Novel Polypeptide from Sea Squirt Ciona Savignyi
    Article Snippet: .. Recombinant Expression of CS5931 from E. coli BL21 (DE3) The expression vector, pET28a/CS5931 synthesized by our lab previously [ ], was transfected into E. coli BL21 (DE3) cells (Novagen, Darmstadt, Germany). .. Cells were incubated in low-salt LB medium (containing 100 µg/mL Kanamycin) at 37 °C with shaking at 200 rpm.

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  • 99
    Millipore bl21
    Bl21, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 62 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bl21/product/Millipore
    Average 99 stars, based on 62 article reviews
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    Millipore rosetta bl21
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    Millipore bl21 de3 plyss rosetta
    Bl21 De3 Plyss Rosetta, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore bl21 de3 plyss cells
    Bl21 De3 Plyss Cells, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 94 stars, based on 10 article reviews
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    bl21 de3 plyss cells - by Bioz Stars, 2020-04
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