bl21 gold  (Thermo Fisher)


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    Structured Review

    Thermo Fisher bl21 gold
    Bl21 Gold, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bl21 gold/product/Thermo Fisher
    Average 94 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    bl21 gold - by Bioz Stars, 2020-02
    94/100 stars

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    Related Articles

    Amplification:

    Article Title: Positive and Negative Regulation of the Cardiovascular Transcription Factor KLF5 by p300 and the Oncogenic Regulator SET through Interaction and Acetylation on the DNA-Binding Domain
    Article Snippet: Glutathione S -transferase (GST)-tagged constructs KLF5 wt (wild type), KLF5-ΔDBD, KLF5-DBD, KLF5-zinc finger 1, KLF5-zinc finger 2, and KLF-zinc finger 3 were similarly PCR amplified and inserted into pGEX vectors (Amersham Pharmacia Biotech) ( , ). .. Hexahistidine-tagged SET/TAF-Iβ construct ( ) (a kind gift of K. Nagata) was transformed into the BL21-Gold(DE3)pLysS strain, induced, and then purified with Probond resin (Invitrogen) and buffer C containing 20 mM imidazole for washing and 200 mM imidazole for elution similar that described previously ( ).

    Synthesized:

    Article Title: Positive and Negative Regulation of the Cardiovascular Transcription Factor KLF5 by p300 and the Oncogenic Regulator SET through Interaction and Acetylation on the DNA-Binding Domain
    Article Snippet: Hexahistidine-tagged SET/TAF-Iβ construct ( ) (a kind gift of K. Nagata) was transformed into the BL21-Gold(DE3)pLysS strain, induced, and then purified with Probond resin (Invitrogen) and buffer C containing 20 mM imidazole for washing and 200 mM imidazole for elution similar that described previously ( ). .. Zinc finger peptides were synthesized commercially by Hokkaido System Science Co. Ltd.

    Mutagenesis:

    Article Title: Positive and Negative Regulation of the Cardiovascular Transcription Factor KLF5 by p300 and the Oncogenic Regulator SET through Interaction and Acetylation on the DNA-Binding Domain
    Article Snippet: Hexahistidine-tagged SET/TAF-Iβ construct ( ) (a kind gift of K. Nagata) was transformed into the BL21-Gold(DE3)pLysS strain, induced, and then purified with Probond resin (Invitrogen) and buffer C containing 20 mM imidazole for washing and 200 mM imidazole for elution similar that described previously ( ). .. SET/TAF-Iβ deletion constructs were in part a generous gift from K. Nagata, otherwise they were constructed by PCR mutagenesis.

    Construct:

    Article Title: Positive and Negative Regulation of the Cardiovascular Transcription Factor KLF5 by p300 and the Oncogenic Regulator SET through Interaction and Acetylation on the DNA-Binding Domain
    Article Snippet: .. Hexahistidine-tagged SET/TAF-Iβ construct ( ) (a kind gift of K. Nagata) was transformed into the BL21-Gold(DE3)pLysS strain, induced, and then purified with Probond resin (Invitrogen) and buffer C containing 20 mM imidazole for washing and 200 mM imidazole for elution similar that described previously ( ). .. SET/TAF-Iβ deletion constructs were in part a generous gift from K. Nagata, otherwise they were constructed by PCR mutagenesis.

    Article Title: Arrestin Expression in E. coli and Purification
    Article Snippet: .. The corresponding constructs are transformed into the commercially available BL21 GOLD (Invitrogen) competent cells according to the manufacturer’s instructions. ..

    Purification:

    Article Title: Positive and Negative Regulation of the Cardiovascular Transcription Factor KLF5 by p300 and the Oncogenic Regulator SET through Interaction and Acetylation on the DNA-Binding Domain
    Article Snippet: .. Hexahistidine-tagged SET/TAF-Iβ construct ( ) (a kind gift of K. Nagata) was transformed into the BL21-Gold(DE3)pLysS strain, induced, and then purified with Probond resin (Invitrogen) and buffer C containing 20 mM imidazole for washing and 200 mM imidazole for elution similar that described previously ( ). .. SET/TAF-Iβ deletion constructs were in part a generous gift from K. Nagata, otherwise they were constructed by PCR mutagenesis.

    Protein Purification:

    Article Title: In Vitro Analysis of RNA Degradation Catalyzed by Deadenylase Enzymes
    Article Snippet: Paragraph title: 2.1. Protein Purification Components ... We use BL21 Gold (Invitrogen) or KRX (Promega) strains, unless a specialized strain is required.

    Polymerase Chain Reaction:

    Article Title: Positive and Negative Regulation of the Cardiovascular Transcription Factor KLF5 by p300 and the Oncogenic Regulator SET through Interaction and Acetylation on the DNA-Binding Domain
    Article Snippet: Glutathione S -transferase (GST)-tagged constructs KLF5 wt (wild type), KLF5-ΔDBD, KLF5-DBD, KLF5-zinc finger 1, KLF5-zinc finger 2, and KLF-zinc finger 3 were similarly PCR amplified and inserted into pGEX vectors (Amersham Pharmacia Biotech) ( , ). .. Hexahistidine-tagged SET/TAF-Iβ construct ( ) (a kind gift of K. Nagata) was transformed into the BL21-Gold(DE3)pLysS strain, induced, and then purified with Probond resin (Invitrogen) and buffer C containing 20 mM imidazole for washing and 200 mM imidazole for elution similar that described previously ( ).

    HAT Assay:

    Article Title: Positive and Negative Regulation of the Cardiovascular Transcription Factor KLF5 by p300 and the Oncogenic Regulator SET through Interaction and Acetylation on the DNA-Binding Domain
    Article Snippet: Hexahistidine-tagged SET/TAF-Iβ construct ( ) (a kind gift of K. Nagata) was transformed into the BL21-Gold(DE3)pLysS strain, induced, and then purified with Probond resin (Invitrogen) and buffer C containing 20 mM imidazole for washing and 200 mM imidazole for elution similar that described previously ( ). .. The p300 histone acetyltransferase (HAT) domain constructs have been described previously ( ) (a kind gift of Y. Nakatani).

    Expressing:

    Article Title: Positive and Negative Regulation of the Cardiovascular Transcription Factor KLF5 by p300 and the Oncogenic Regulator SET through Interaction and Acetylation on the DNA-Binding Domain
    Article Snippet: Protein expression and purification were done essentially as described previously, with modification by use of HiTrap heparin and HisTrap columns (Amersham Pharmacia Biotech) ( ). .. Hexahistidine-tagged SET/TAF-Iβ construct ( ) (a kind gift of K. Nagata) was transformed into the BL21-Gold(DE3)pLysS strain, induced, and then purified with Probond resin (Invitrogen) and buffer C containing 20 mM imidazole for washing and 200 mM imidazole for elution similar that described previously ( ).

    Article Title: Arrestin Expression in E. coli and Purification
    Article Snippet: Paragraph title: Step 1. Expression of arrestin proteins in Escherichia coli (BL-21 GOLD) ... The corresponding constructs are transformed into the commercially available BL21 GOLD (Invitrogen) competent cells according to the manufacturer’s instructions.

    Article Title: In Vitro Analysis of RNA Degradation Catalyzed by Deadenylase Enzymes
    Article Snippet: Protease deficient E. coli strain transformed with deadenylase expression vector. .. We use BL21 Gold (Invitrogen) or KRX (Promega) strains, unless a specialized strain is required.

    Modification:

    Article Title: Positive and Negative Regulation of the Cardiovascular Transcription Factor KLF5 by p300 and the Oncogenic Regulator SET through Interaction and Acetylation on the DNA-Binding Domain
    Article Snippet: Protein expression and purification were done essentially as described previously, with modification by use of HiTrap heparin and HisTrap columns (Amersham Pharmacia Biotech) ( ). .. Hexahistidine-tagged SET/TAF-Iβ construct ( ) (a kind gift of K. Nagata) was transformed into the BL21-Gold(DE3)pLysS strain, induced, and then purified with Probond resin (Invitrogen) and buffer C containing 20 mM imidazole for washing and 200 mM imidazole for elution similar that described previously ( ).

    Transformation Assay:

    Article Title: Positive and Negative Regulation of the Cardiovascular Transcription Factor KLF5 by p300 and the Oncogenic Regulator SET through Interaction and Acetylation on the DNA-Binding Domain
    Article Snippet: .. Hexahistidine-tagged SET/TAF-Iβ construct ( ) (a kind gift of K. Nagata) was transformed into the BL21-Gold(DE3)pLysS strain, induced, and then purified with Probond resin (Invitrogen) and buffer C containing 20 mM imidazole for washing and 200 mM imidazole for elution similar that described previously ( ). .. SET/TAF-Iβ deletion constructs were in part a generous gift from K. Nagata, otherwise they were constructed by PCR mutagenesis.

    Article Title: Arrestin Expression in E. coli and Purification
    Article Snippet: .. The corresponding constructs are transformed into the commercially available BL21 GOLD (Invitrogen) competent cells according to the manufacturer’s instructions. ..

    Article Title: In Vitro Analysis of RNA Degradation Catalyzed by Deadenylase Enzymes
    Article Snippet: Protease deficient E. coli strain transformed with deadenylase expression vector. .. We use BL21 Gold (Invitrogen) or KRX (Promega) strains, unless a specialized strain is required.

    Recombinant:

    Article Title: Positive and Negative Regulation of the Cardiovascular Transcription Factor KLF5 by p300 and the Oncogenic Regulator SET through Interaction and Acetylation on the DNA-Binding Domain
    Article Snippet: Paragraph title: Preparation of recombinant epitope-tagged protein. ... Hexahistidine-tagged SET/TAF-Iβ construct ( ) (a kind gift of K. Nagata) was transformed into the BL21-Gold(DE3)pLysS strain, induced, and then purified with Probond resin (Invitrogen) and buffer C containing 20 mM imidazole for washing and 200 mM imidazole for elution similar that described previously ( ).

    Plasmid Preparation:

    Article Title: Arrestin Expression in E. coli and Purification
    Article Snippet: All arrestin coding sequences can be subcloned into pTrcHisB vector (Life technologies) between Nco I and Hind III sites (this removes the His tag) as described ( ; ). .. The corresponding constructs are transformed into the commercially available BL21 GOLD (Invitrogen) competent cells according to the manufacturer’s instructions.

    Article Title: In Vitro Analysis of RNA Degradation Catalyzed by Deadenylase Enzymes
    Article Snippet: Protease deficient E. coli strain transformed with deadenylase expression vector. .. We use BL21 Gold (Invitrogen) or KRX (Promega) strains, unless a specialized strain is required.

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  • 99
    Thermo Fisher e coli bl21 gold cells
    E Coli Bl21 Gold Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e coli bl21 gold cells/product/Thermo Fisher
    Average 99 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    e coli bl21 gold cells - by Bioz Stars, 2020-02
    99/100 stars
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