bl21 de3 trxb  (Millipore)


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  • 84

    Structured Review

    Millipore bl21 de3 trxb
    Bl21 De3 Trxb, supplied by Millipore, used in various techniques. Bioz Stars score: 84/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bl21 de3 trxb/product/Millipore
    Average 84 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    bl21 de3 trxb - by Bioz Stars, 2020-03
    84/100 stars

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    Related Articles

    Construct:

    Article Title: Distinct activation modes of the Relaxin Family Peptide Receptor 2 in response to insulin-like peptide 3 and relaxin
    Article Snippet: .. The construct was expressed in BL21 (DE3) trxB (Novagen) cells using autoinduction . .. N5052 medium was used for uniform 15 N-labelling using 15 NH4 Cl (Sigma-Aldrich) and the method described in was employed for 13 C, 15 N labelling, with cells grown in a 1 L Braun Biostat fermenter, and 15 NH4 Cl and D-[13 C] glucose being the only nitrogen and carbon sources.

    Purification:

    Article Title: Distinct activation modes of the Relaxin Family Peptide Receptor 2 in response to insulin-like peptide 3 and relaxin
    Article Snippet: Paragraph title: Expression and purification of RXFP2(1–65) ... The construct was expressed in BL21 (DE3) trxB (Novagen) cells using autoinduction .

    Article Title: The complex binding mode of the peptide hormone H2 relaxin to its receptor RXFP1
    Article Snippet: Paragraph title: Expression and purification of RXFP1(1–72) ... Briefly, protein was expressed into BL21 (DE3) trxB (Novagen) using autoinduction where protein expression via the T7 promoter is regulated by levels of lactose/glucose in the medium .

    Isotopic Labeling:

    Article Title: The complex binding mode of the peptide hormone H2 relaxin to its receptor RXFP1
    Article Snippet: Briefly, protein was expressed into BL21 (DE3) trxB (Novagen) using autoinduction where protein expression via the T7 promoter is regulated by levels of lactose/glucose in the medium . .. For uniform 15 N isotopic labelling, GB1-RXFP1(1–72) was expressed in N5052 minimal medium using 15 NH4 Cl (Sigma-Aldrich).

    Solubility:

    Article Title: Distinct activation modes of the Relaxin Family Peptide Receptor 2 in response to insulin-like peptide 3 and relaxin
    Article Snippet: Expression and purification of RXFP2(1–65) RXFP2(1–65) was designed with a GB1 solubility tag on both the N- and C-termini as well as an N-terminal His6 tag which was removable along with the N-terminal GB1 by thrombin cleavage. .. The construct was expressed in BL21 (DE3) trxB (Novagen) cells using autoinduction .

    Expressing:

    Article Title: Distinct activation modes of the Relaxin Family Peptide Receptor 2 in response to insulin-like peptide 3 and relaxin
    Article Snippet: Paragraph title: Expression and purification of RXFP2(1–65) ... The construct was expressed in BL21 (DE3) trxB (Novagen) cells using autoinduction .

    Article Title: The complex binding mode of the peptide hormone H2 relaxin to its receptor RXFP1
    Article Snippet: .. Briefly, protein was expressed into BL21 (DE3) trxB (Novagen) using autoinduction where protein expression via the T7 promoter is regulated by levels of lactose/glucose in the medium . .. For uniform 15 N isotopic labelling, GB1-RXFP1(1–72) was expressed in N5052 minimal medium using 15 NH4 Cl (Sigma-Aldrich).